A culture bag with an inserted optical fiber optical sensing patch electrode detection structure

Abstract

The utility model provides a kind of culture bag with insertion type optical fiber light feeling patch electrode detection structure, including culture bag body, and optical sensor, optical sensor includes optical fiber sensor, and the fixed structure for fixing optical fiber sensor is set on culture bag body, fixed structure includes the double-end connecting disc that is set on culture bag body, inner pipeline and outer pipeline, one end of inner pipeline is sleeved with electrode mounting connector, electrode mounting connector is made of light-transmitting material, outer pipeline, double-end connecting disc and inner pipeline are connected in series to form optical fiber channel for passing through optical fiber, one end of optical fiber is inserted into optical fiber channel and is connected with electrode mounting connector one end.The culture bag with insertion type optical fiber light feeling patch electrode detection structure in the utility model, by setting fixed structure, optical sensor is introduced to the inside of culture bag body, internal liquid environment is directly detected, to improve the accuracy of detection result.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical technology, and in particular to a culture bag with an insertable fiber optic photosensitive patch electrode detection structure. Background Technology

[0002] In biopharmaceuticals and cell therapy, wave-type bioreactors provide a mild, low-shear cell culture environment through non-invasive wave-like mixing and disposable sterile cell culture bags, reducing the risk of contamination and simplifying operational procedures. During cell culture, as cell density increases, a large amount of lactic acid is produced and oxygen is consumed. Therefore, it is necessary to monitor and control the pH and dissolved oxygen in the culture environment in real time to ensure optimal conditions for cell growth. Wave-type bioreactors typically use disposable culture bags, which are pre-sterilized, avoiding the cleaning and sterilization problems of traditional reactors. However, this also means that pH and dissolved oxygen sensors need to be pre-installed inside the bags to achieve online monitoring of the culture process. Optical pH and dissolved oxygen sensors are widely used in wave-type bioreactors due to their non-invasiveness, rapid response, and high accuracy. These sensors transmit light signals to transmitters via optical fibers, thereby enabling real-time monitoring of pH and dissolved oxygen in the culture environment. To ensure that the sensors can fully contact the culture medium and monitor accurately, existing designs typically place the optical sensors at the bottom of the culture bag.

[0003] The placement of the optical sensor at the bottom of the culture bag causes light loss when it receives the light source. This is because, in order to ensure the culture temperature within the bag and reduce the impact of the fiber optic design on the heating space at the bottom of the bag, the detection light path in the fiber optic needs to be refracted to the optical electrode at the bottom of the bag for optical reaction. This results in light loss when the light source enters the optical sensor, thus affecting the actual calculation and reading of pH and dissolved oxygen. Furthermore, the concave design of the optical sensor relative to the bottom plane of the bag often leads to two situations during the wave-shaped bioreactor culture process that can affect the actual reading of pH and dissolved oxygen. Utility Model Content

[0004] To address the shortcomings of existing technologies, the purpose of this invention is to provide a culture bag with an insertable fiber optic photosensitive patch electrode detection structure, thereby solving the technical problems described in the background section.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution:

[0006] A culture bag with an insertable fiber optic sensor patch electrode detection structure is used to place on a wave-type bioreactor. It includes a culture bag body and an optical sensor disposed on the culture bag body. The optical sensor includes a fiber optic sensor for detecting pH or dissolved oxygen in the culture environment inside the culture bag, and a fixing structure disposed on the culture bag body for fixing the fiber optic sensor. The fixing structure includes a double-ended connecting plate disposed on the culture bag body. The double-ended connecting plate includes a first connector located inside the culture bag body and a second connector located outside the culture bag body. The first connector and the second connector are connected in communication. The first connector is sealed to an inner tube, and the second connector is connected to an outer tube. The end of the inner tube away from the first connector is sealed to an electrode mounting connector, which is made of a light-transmitting material. The outer tube, the double-ended connecting plate, and the inner tube, which are connected in series, form a fiber optic channel for the passage of optical fibers. One end of the optical fiber extends into the fiber optic channel and connects to one end of the electrode mounting connector.

[0007] Compared with the prior art, the beneficial effects of this utility model are as follows: By setting a fixing structure on the culture bag body, wherein the fixing structure includes a double-headed connecting plate that is sealed to the culture bag body, the double-headed connecting plate connects the inner pipeline and the outer pipeline through a first connector set inside the culture bag body and a second connector set outside the culture bag body, forming an optical fiber channel inside, thereby separating the culture space inside the culture bag body from the outside while introducing optical fiber into the culture bag, realizing indirect detection of pH or dissolved oxygen in the internal environment, avoiding the light loss caused by light source refraction due to the optical sensor being set at the bottom of the culture bag, and the problem of poor detection accuracy caused by the bottom of the culture bag being concave due to the setting at the bottom of the culture bag.

[0008] According to one aspect of the above technical solution, the culture bag body includes an upper membrane portion and a lower membrane portion, and the double-headed connecting disc is disposed on the upper membrane portion.

[0009] According to one aspect of the above technical solution, the lower membrane portion is provided with a fixing diaphragm for fixing the inner pipeline.

[0010] According to one aspect of the above technical solution, the fixed diaphragm includes an integrally formed connecting part and a fixing part. One side of the connecting part is connected to the lower diaphragm part, and the fixing part is provided with a through hole, through which the inner pipeline passes.

[0011] According to one aspect of the above technical solution, the fixing part is located near the middle of the inner pipeline.

[0012] According to one aspect of the above technical solution, the integrally formed electrode mounting connector includes an open end and a light-transmitting end disposed opposite to each other. The open end is located at the end of the electrode mounting connector closer to the inner pipeline, and the light-transmitting end is located at the end of the electrode mounting connector away from the inner pipeline.

[0013] According to one aspect of the above technical solution, a fixing clip for fixing the optical fiber to the outer conduit is provided on the outer conduit.

[0014] According to one aspect of the above technical solution, the culture bag body is provided with two optical sensors, the two optical sensors respectively including a pH sensor and a dissolved oxygen sensor for detecting the pH and dissolved oxygen of the culture environment inside the culture bag. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of a culture bag with an insertable fiber optic photosensitive patch electrode detection structure according to one embodiment of the present invention;

[0016] Figure 2 This is a schematic diagram of the fixing structure in one embodiment of the present invention;

[0017] Figure 3 This is a schematic diagram of the electrode mounting connector in one embodiment of the present invention;

[0018] Explanation of main component symbols: 1-Cultivation bag body, 11-Lower membrane part, 2-Fixing structure, 21-External tubing, 22-Double-head connecting plate, 23-Internal tubing, 24-Electrode mounting connector, 241-Open end, 242-Light-transmitting end, 3-Fixing membrane, 31-Fixing part, 311-Through hole, 32-Connecting part, 4-Fixing clip, 5-Fiber optic cable;

[0019] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this utility model. Detailed Implementation

[0020] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. Several embodiments of this utility model are shown in the drawings. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of this utility model will be more thorough and complete.

[0021] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or there may be an intervening component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there may be an intervening component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] See Figures 1 to 3 An embodiment of this utility model provides a culture bag with an insertable fiber optic photosensitive patch electrode detection structure, which is placed on a wave-type bioreactor for cell culture. It includes a culture bag body 1 and two fixing structures 2 disposed on the culture bag body 1. Optical sensors are inserted in the fixing structures 2 for fixing a pH sensor and a dissolved oxygen sensor inside the culture bag to detect the pH and dissolved oxygen values ​​of the liquid environment.

[0024] The fixed structure 2 includes an outer pipe 21, a double-ended connecting plate 22, an inner pipe 23, and an electrode mounting connector 24 connected in series. The double-ended connecting plate 22 is fixedly connected to the culture bag body 1. The double-ended connecting plate 22 is an integrally formed structure, including a first connector and a second connector arranged opposite to each other. The first connector is located inside the culture bag body 1 and is sealed to one end of the inner pipe 23, while the second connector is located outside the culture bag and connects to the outer pipe 21. The double-ended connecting plate 22 is hollow, thereby connecting the outer pipe 21 and the inner pipe 23. The electrode mounting connector 24 is connected to the other end of the inner pipe 23. The electrode mounting connector 24 is integrally formed and has an open end, including an open end 241 and a light-transmitting end 242. The open end 241 is inserted into one end of the inner pipe 23 and connected to the inner pipe. The inner tube 23 is sealed, and the light-transmitting end 242 is located outside one end of the inner tube 23, sealing off one end opening of the inner tube 23 to isolate it from the internal environment of the culture bag body 1. It can be understood that the series-connected outer tube 21, double-ended connecting plate 22, inner tube 23, and electrode mounting connector 24 form an optical fiber channel isolated from the internal environment of the culture bag body 1. The optical fiber of the optical sensor extends into the middle of the internal environment of the culture bag body 1 through the optical fiber channel, with one end connected to the electrode mounting connector 24, enabling direct optical detection of the pH or dissolved oxygen of the culture environment inside the culture bag body 1. It can be understood that by setting the fixing structure 2, the light source detection part of the optical sensor is introduced into the interior of the culture bag body 1 for direct detection, thereby improving the detection accuracy. Furthermore, it should be noted that, to improve detection accuracy, the electrode mounting connector 24 is made of a high-transmittance material.

[0025] Furthermore, in the embodiment, the culture bag body 1 is formed by high-temperature fusion of the upper membrane part and the lower membrane part 11 at the four edges. When the culture bag body 1 is used in conjunction with the wave-type bioreactor, the inside of the culture bag body 1 is filled with air, so that the culture bag body 1 has a pillow-shaped structure. In order to improve the stability of the culture bag body 1 and the operability of the optical sensor, the double-headed connecting plate 22 is set on the upper membrane part for easy control.

[0026] During cell culture, the liquid inside the culture bag body 1 oscillates within the body due to the movement of the wave-like bioreactor, causing the inner tubing 23 to shake and thus affecting the detection results. Therefore, a fixing membrane 3 is provided on the side of the lower membrane section 11 near the inside of the culture bag body 1 to fix the inner tubing 23 to one side of the lower membrane section 11, reducing the shaking of the inner tubing 23. In this embodiment, the fixing membrane 3 includes an integrally formed connecting part 32 and a fixing part 31. One side of the connecting part 32 is connected to the lower membrane section 11, and the fixing part 31 has a through hole 311 through which the inner tubing 23 passes. The fixing part 31 is located near the inside of the culture bag body 1. The inner tube 23 is located in the middle. Of course, the fixing diaphragm 3 is not limited to the structure described in the embodiment. It can also be that two connecting parts 32 are provided at both ends of the fixing part 31, and the inner tube 23 is fixed by the two connecting parts 32 to improve the stability of the inner tube 23. Those skilled in the art can design according to the actual situation. By setting the double-headed connecting plate 22 in the upper membrane part and cooperating with the fixing diaphragm 3 set in the lower membrane part 3, the inner tube 23 reduces the contact area with the internal liquid while ensuring that the light-transmitting end 242 of the electrode mounting connector 24 is in contact with the lower membrane part 11, so that the optical sensor can also have better contact with the liquid in the bag when the small volume is cultured.

[0027] A fixing clip 4 is fitted onto the outer conduit 21. When fixing, simply press the fixing clip 4 to press the optical fiber 5 into the outer conduit 21, thereby preventing the optical fiber 5 from moving inside the optical fiber channel and ensuring that one end of the optical fiber 5 is stably connected to the electrode mounting connector 24.

[0028] In summary, the culture bag with an insertable fiber optic sensor patch electrode detection structure in the above embodiments of this utility model improves the detection accuracy by introducing the detection end of the optical sensor into the interior of the culture bag body through a fixed structure.

[0029] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0030] The above-described embodiments are merely illustrative of several implementations of this utility model, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of this utility model. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the appended claims.

Claims

1. A culture bag having an inserted optical fiber optical sensing patch electrode detection structure for placement on a wave bioreactor, comprising a culture bag body, and an optical sensor disposed on the culture bag body, characterized in that, The optical sensor includes an optical fiber sensor for detecting pH or dissolved oxygen in the culture environment inside the culture bag, and a fixing structure disposed on the culture bag body for fixing the optical fiber sensor. The fixing structure includes a double-ended connecting plate disposed on the culture bag body. The double-ended connecting plate includes a first connector portion located inside the culture bag body and a second connector portion located outside the culture bag body. The first connector portion and the second connector portion are connected in communication. The first connector portion is sealed to an inner tube, and the second connector portion is connected to an outer tube. The end of the inner tube away from the first connector portion is sealed to an electrode mounting connector. The electrode mounting connector is made of a light-transmitting material. The outer tube, the double-ended connecting plate, and the inner tube, which are connected in series, form an optical fiber channel for passing optical fiber. One end of the optical fiber extends into the optical fiber channel and is connected to one end of the electrode mounting connector.

2. The culture bag having an inserted fiber-optic light-sensing patch electrode detection structure according to claim 1, characterized in that, The culture bag body includes an upper membrane section and a lower membrane section, and the double-headed connecting plate is disposed on the upper membrane section.

3. The culture bag having an inserted fiber-optic light-sensing patch electrode detection structure according to claim 2, characterized in that, The lower membrane section is provided with a fixing diaphragm for fixing the inner pipeline.

4. The culture bag having an inserted fiber-optic light-sensing patch electrode detection structure according to claim 3, characterized in that, The fixed diaphragm includes an integrally formed connecting part and a fixing part. One side of the connecting part is connected to the lower diaphragm part, and the fixing part is provided with a through hole, through which the inner pipeline passes.

5. The culture bag having an inserted fiber-optic light-sensing patch electrode detection structure according to claim 4, wherein, The fixing part is located near the front middle part of the inner pipeline.

6. The culture bag with the inserted fiber-optic light-sensing patch electrode detection structure according to any one of claims 1-5, characterized in that, The integrally formed electrode mounting connector includes an open end and a light-transmitting end that are disposed opposite to each other. The open end is located at the end of the electrode mounting connector that is closer to the inner conduit, and the light-transmitting end is located at the end of the electrode mounting connector that is farther away from the inner conduit.

7. The culture bag with the inserted fiber-optic light-sensing patch electrode detection structure according to any one of claims 1-5, characterized in that, The outer conduit is fitted with a fixing clip for fixing the optical fiber to the outer conduit.

8. The culture bag having an insert-type fiber-optic light-sensing patch electrode detection structure according to claim 1, characterized by, The culture bag is equipped with two optical sensors, which include a pH sensor and a dissolved oxygen sensor for detecting the pH and dissolved oxygen of the culture environment inside the culture bag.

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