Three-dimensional cell culture and microenvironment simulation device

Abstract

The utility model relates to cell culture device technical field, concretely to cell three -dimensional culture and microenvironment simulation device, including environment simulation box, be equipped with lifting assembly in environment simulation box, and lifting assembly includes the circular fixed vertical tube of fixed connection in the bottom of environment simulation box inner wall, is equipped with electric jar in circular fixed vertical tube, and the movable rod end portion of electric jar is equipped with circular support sleeve, and the top of circular support sleeve outer wall is equipped with a plurality of rotation parts with annular array arrangement, and the rotation part includes the support plate of fixed connection in circular support sleeve outer wall, and the bottom of support plate is equipped with motor, and the output shaft of motor passes through the bottom of support plate and coaxially connected with rotating plate, and the top of rotating plate places the culture dish, and the three -dimensional cell culture support is placed in culture dish. This cell three -dimensional culture and microenvironment simulation device, through the rotation part and support plate design of annular array arrangement, can accommodate multiple culture dishes simultaneously, and the parallel culture of experimental group and control group is convenient.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, specifically a three-dimensional cell culture and microenvironment simulation device. Background Technology

[0002] Cell culture is an experimental technique widely used in cell biology, drug development, tissue engineering, and regenerative medicine. It provides suitable growth conditions for isolated cells by simulating the in vivo environment to study cell growth, differentiation, proliferation, and responses to external stimuli. While traditional two-dimensional cell culture techniques are simple and easy to operate, the adherent growth pattern of cells cannot fully simulate the three-dimensional microenvironment of cells in vivo, leading to significant discrepancies between experimental results and actual physiological conditions. Three-dimensional cell culture technology provides a three-dimensional structure that closely resembles the in vivo tissue environment, allowing cells to grow and interact in three-dimensional space, significantly improving the realism and reliability of experiments. However, existing three-dimensional cell culture devices still have limitations in simulating the microenvironment.

[0003] Utility model patent CN214115542U discloses a cell microenvironment simulation device, including an inner box. An outer heating shell is fixedly connected to the outer side of the inner box, and an outer heat insulation shell is fixedly connected to the outer side of the outer heating shell. Five heating tubes are fixedly connected to the inner wall of the outer heating shell. A hydraulic push rod is fixedly connected to the right side of the outer heat insulation shell, and a cover plate is fixedly connected to the top of the hydraulic push rod. Two guide rods are fixedly connected to the inner bottom wall of the inner box, and guide cylinders are slidably connected to the outer sides of the guide rods. A base is fixedly connected between the tops of the two guide cylinders, and a culture dish is inserted into the top of the base. A drive motor is fixedly connected to the inner bottom wall of the inner box, and a threaded rod is fixedly connected to the output shaft of the drive motor. This cell microenvironment simulation device facilitates the handling of culture dishes, provides good temperature retention, and allows for easy temperature control and oxygen content adjustment.

[0004] The existing technology simulates the cellular microenvironment within an inner chamber and uses a drive motor to rotate a threaded rod, which in turn raises a lifting cylinder, pushing the culture dish to near the top of the inner chamber for easy retrieval. However, this device has the following limitations: its structural design can only accommodate a single culture dish, making it unsuitable for comparative studies of experimental and control groups; furthermore, the device lacks rotation functionality, failing to ensure sufficient dispersion and interaction between the culture medium and the three-dimensional cell culture scaffold. Therefore, we propose a three-dimensional cell culture and microenvironment simulation device to overcome the shortcomings of existing technologies and solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a three-dimensional cell culture and microenvironment simulation device to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: A three-dimensional cell culture and microenvironment simulation device includes an environment simulation chamber. The environment simulation chamber provides a stable microenvironment required for cell culture and integrates temperature, humidity, and gas concentration control functions. Its specific working principle is based on existing technology, such as the detailed working principle of a cell microenvironment simulation device disclosed in utility model patent CN214115542U, which will not be repeated here. The environment simulation chamber is equipped with a lifting component to facilitate the handling of culture dishes. The lifting component includes a circular fixed vertical tube fixedly connected to the bottom of the inner wall of the environment simulation chamber. An electric cylinder is installed in the circular fixed vertical tube. The electric cylinder drives the circular support sleeve to rise and fall through the extension and retraction of a movable rod. The end of the movable rod of the electric cylinder is equipped with a circular support sleeve. The top of the outer wall of the circular support sleeve is equipped with multiple rotating parts arranged in a ring array. The rotating parts promote the uniform interaction between the culture medium and the three-dimensional cell culture scaffold through rotation. The rotating component includes a support plate fixedly connected to the outer wall of a circular support sleeve. The support plate provides an installation platform for the motor and the rotating plate. A motor is located at the bottom of the support plate, which drives the rotating plate to rotate to improve cell distribution. The output shaft of the motor passes through the bottom of the support plate and is coaxially connected to the rotating plate. The rotating plate drives the culture dish to move by rotating. A culture dish is placed on the top of the rotating plate. The culture dish is used to contain culture medium and a three-dimensional cell culture scaffold. The three-dimensional cell culture scaffold is placed inside the culture dish. The three-dimensional cell culture scaffold provides a three-dimensional growth space to promote cell adhesion and proliferation.

[0007] Preferably, an electric lifting rod is vertically installed on one side of the environmental simulation chamber. The electric lifting rod controls the opening and closing of the cover plate to ensure convenient operation. The movable end of the electric lifting rod is provided with a cover plate, which seals the top of the environmental simulation chamber to reduce external interference.

[0008] Preferably, the circular support sleeve is located on the outside of the circular fixed vertical tube, and the inner wall of the circular support sleeve is in contact with the outer wall of the circular fixed vertical tube. The cooperative design between the circular support sleeve and the circular fixed vertical tube ensures the stability of the lifting process.

[0009] Preferably, the bottom of the support plate is provided with a reinforcing diagonal brace, the bottom end of which is fixedly connected to the outer wall of the circular support sleeve, thereby enhancing the connection strength between the support plate and the circular support sleeve.

[0010] Preferably, the bottom of the rotating plate has a plurality of rectangular grooves arranged in a circular array. The rectangular grooves accommodate the rollers and reduce rotational friction. The rollers are rotatably connected in the rectangular grooves and roll on the top of the support plate. The rolling of the rollers reduces the contact resistance between the rotating plate and the support plate.

[0011] Preferably, the top of the rotating plate has four positioning slots arranged in a matrix. The depth of the positioning slots is half the thickness of the rotating plate. The positioning slots cooperate with the positioning blocks to fix the culture dish and prevent rotational displacement.

[0012] Preferably, the bottom of the culture dish is provided with four positioning blocks, which are respectively inserted into four positioning slots to ensure that the position of the culture dish is fixed.

[0013] Compared with the prior art, the beneficial effects of this utility model are: 1. This three-dimensional cell culture and microenvironment simulation device, through the design of rotating components and support plates arranged in a ring array, can simultaneously accommodate multiple culture dishes, facilitating parallel culture of experimental and control groups; combined with the rotating plate function driven by a motor, it realizes the periodic rotation of the culture dishes, promotes full interaction between the culture medium and the three-dimensional cell culture scaffold, and improves the uniformity of cell distribution.

[0014] 2. The three-dimensional cell culture and microenvironment simulation device features a roller design at the bottom of the rotating plate, which effectively reduces friction during rotation; the reinforced diagonal brace enhances the connection strength between the support plate and the circular support sleeve, preventing mechanical vibration caused by rotation. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a partial structural schematic diagram of the present invention; Figure 3 This is a schematic diagram of the assembly structure of the lifting component and the rotating component in this utility model; Figure 4 This is an exploded structural diagram of the rotating component in this utility model; Figure 5 This is a partial structural diagram of the rotating component in this utility model; Figure 6 This is a schematic diagram of the petri dish structure in this utility model; In the diagram: 100, Environmental simulation chamber; 200, Electric lifting rod; 300, Cover plate; 400, Lifting assembly; 410, Circular fixed vertical tube; 420, Electric cylinder; 430, Circular support sleeve; 500, Rotating component; 510, Support plate; 520, Motor; 530, Rotating plate; 531, Positioning slot; 532, Rectangular groove; 540, Roller; 550, Reinforcing diagonal bar; 600, Culture dish; 610, Positioning insert; 700, Three-dimensional cell culture scaffold. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0018] Please see Figures 1-6 This utility model provides a technical solution: A three-dimensional cell culture and microenvironment simulation device includes an environment simulation chamber 100. The environment simulation chamber 100 is used to provide a stable microenvironment required for cell culture and integrates temperature, humidity and gas concentration control functions. Its specific working principle is existing technology, such as the detailed working principle of a cell microenvironment simulation device disclosed in utility model patent with announcement number CN214115542U, which will not be repeated here. The environment simulation chamber 100 is provided with a lifting component 400 to facilitate the placement and removal of culture dishes 600. The lifting component 400 includes a circular fixed vertical tube 410 fixedly connected to the bottom of the inner wall of the environment simulation chamber 100. An electric cylinder 420 is provided in the circular fixed vertical tube 410. The electric cylinder 420 drives the circular support sleeve 430 to rise and fall through the extension and retraction of the movable rod. The end of the movable rod of the electric cylinder 420 is provided with a circular support sleeve 430. The top of the outer wall of the circular support sleeve 430 is provided with multiple rotating parts 500 arranged in a ring array. The rotating parts 500 promote the uniform interaction between the culture medium and the three-dimensional cell culture scaffold 700 through the rotation function. The rotating component 500 includes a support plate 510 fixedly connected to the outer wall of the circular support sleeve 430. The support plate 510 provides a mounting platform for the motor 520 and the rotating plate 530. The motor 520 is located at the bottom of the support plate 510. The motor 520 drives the rotating plate 530 to rotate to improve cell distribution. The output shaft of the motor 520 passes through the bottom of the support plate 510 and is coaxially connected to the rotating plate 530. The rotating plate 530 drives the culture dish 600 to move by rotating. The culture dish 600 is placed on the top of the rotating plate 530. The culture dish 600 is used to contain culture medium and a three-dimensional cell culture scaffold 700. The three-dimensional cell culture scaffold 700 is placed inside the culture dish 600. The three-dimensional cell culture scaffold 700 provides a three-dimensional growth space to promote cell adhesion and proliferation.

[0019] In this embodiment, an electric lifting rod 200 is vertically installed on one side of the environmental simulation box 100. The electric lifting rod 200 is opened and closed by a lifting control cover plate 300 to ensure convenient operation. The movable rod end of the electric lifting rod 200 is provided with a cover plate 300, which seals the top of the environmental simulation box 100 to reduce external interference.

[0020] Specifically, the circular support sleeve 430 is located on the outside of the circular fixed vertical tube 410, and the inner wall of the circular support sleeve 430 fits against the outer wall of the circular fixed vertical tube 410. The cooperative design of the circular support sleeve 430 and the circular fixed vertical tube 410 ensures the stability of the lifting process.

[0021] Furthermore, the bottom of the support plate 510 is provided with a reinforcing diagonal brace 550, the bottom end of which is fixedly connected to the outer wall of the circular support sleeve 430. The reinforcing diagonal brace 550 enhances the connection strength between the support plate 510 and the circular support sleeve 430.

[0022] Furthermore, the bottom of the rotating plate 530 is provided with a plurality of rectangular grooves 532 arranged in a circular array. The rectangular grooves 532 accommodate the rollers 540, reducing rotational friction. The rollers 540 are rotatably connected in the rectangular grooves 532. The rollers 540 roll on the top of the support plate 510. The rolling of the rollers 540 reduces the contact resistance between the rotating plate 530 and the support plate 510.

[0023] Furthermore, the top of the rotating plate 530 is provided with four positioning slots 531 arranged in a matrix. The depth of the positioning slots 531 is half the thickness of the rotating plate 530. The positioning slots 531 cooperate with the positioning blocks 610 to fix the culture dish 600 and prevent rotational displacement.

[0024] Furthermore, the bottom of the petri dish 600 is provided with four positioning blocks 610, which are respectively inserted into four positioning slots 531. The positioning blocks 610 are inserted into the positioning slots 531 to ensure that the position of the petri dish 600 is fixed.

[0025] It should be noted that the electric lifting rod 200, electric cylinder 420 and motor 520 in this utility model are all controlled by the control system of the environmental simulation box 100. The environmental simulation box 100 is connected to an external power source. All components are general standard parts or parts known to those skilled in the art. Their structure and principle can be learned by those skilled in the art through technical manuals or conventional experimental methods. The specific connection method should refer to the working sequence of each electrical component in the above working principle to complete the electrical connection. The detailed connection method is a well-known technology in the field. The above mainly introduces the working principle and process, and the electrical control will not be described again.

[0026] In this embodiment, the three-dimensional cell culture and microenvironment simulation device is used as follows: First, the cover 300 is opened by controlling the electric lifting rod 200, revealing the interior of the environment simulation chamber 100. The three-dimensional cell culture scaffold 700 is placed in the culture dish 600 and culture medium is added. The electric cylinder 420 is activated to drive the circular support sleeve 430 to rise vertically along the circular fixed vertical tube 410. Then, the positioning insert 610 at the bottom of the culture dish 600 is aligned with the positioning slot 531 on the rotating plate 530 and inserted to fix the culture dish 600. After that, the electric cylinder 420 is reset, moving the culture dish 600 down into the environment simulation chamber 100. After closing the cover 300, the temperature and humidity are set in the environment simulation chamber 100. The gas concentration parameters are used to simulate the cell growth microenvironment. The rotating plate 530 driven by the motor 520 drives the culture dish 600 to rotate. At this time, the roller 540 rolls on the top of the support plate 510, reducing the frictional resistance between the rotating plate 530 and the support plate 510. During the rotation, the culture medium and the three-dimensional cell culture scaffold 700 interact fully, promoting the uniform distribution of cells in three-dimensional space. After the experiment, the electric lifting rod 200 raises the cover plate 300, the electric cylinder 420 lifts the circular support sleeve 430, and the positioning plug 610 and the positioning slot 531 are separated to remove the culture dish 600, which can then be used for subsequent testing or sample replacement. Throughout the process, the environmental simulation chamber 100 maintains the stability of the microenvironment in real time.

[0027] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A three-dimensional cell culture and microenvironment simulation device, comprising an environmental simulation chamber (100), characterized in that: The environmental simulation chamber (100) is equipped with a lifting assembly (400). The lifting assembly (400) includes a circular fixed vertical tube (410) fixedly connected to the bottom of the inner wall of the environmental simulation chamber (100). An electric cylinder (420) is provided inside the circular fixed vertical tube (410). A circular support sleeve (430) is provided at the end of the movable rod of the electric cylinder (420). A plurality of rotating parts (500) arranged in a ring array are provided on the top of the outer wall of the circular support sleeve (430). The rotating part (500) includes a support plate (510) fixedly connected to the outer wall of the circular support sleeve (430). A motor (520) is provided at the bottom of the support plate (510). The output shaft of the motor (520) passes through the bottom of the support plate (510) and is coaxially connected to a rotating plate (530). A culture dish (600) is placed on the top of the rotating plate (530). A three-dimensional cell culture scaffold (700) is placed inside the culture dish (600).

2. The three-dimensional cell culture and microenvironment simulation device according to claim 1, characterized in that: An electric lifting rod (200) is vertically installed on one side of the environmental simulation box (100), and a cover plate (300) is provided at the end of the movable rod of the electric lifting rod (200).

3. The three-dimensional cell culture and microenvironment simulation device according to claim 1, characterized in that: The circular support sleeve (430) is located on the outside of the circular fixed vertical tube (410), and the inner wall of the circular support sleeve (430) is in contact with the outer wall of the circular fixed vertical tube (410).

4. The three-dimensional cell culture and microenvironment simulation device according to claim 1, characterized in that: The bottom of the support plate (510) is provided with a reinforcing diagonal bar (550), and the bottom end of the reinforcing diagonal bar (550) is fixedly connected to the outer wall of the circular support sleeve (430).

5. The three-dimensional cell culture and microenvironment simulation device according to claim 1, characterized in that: The bottom of the rotating plate (530) is provided with a plurality of rectangular grooves (532) arranged in a circular array. Rollers (540) are rotatably connected in the rectangular grooves (532) and the rollers (540) roll on the top of the support plate (510).

6. The three-dimensional cell culture and microenvironment simulation device according to claim 1, characterized in that: The top of the rotating plate (530) has four positioning slots (531) arranged in a matrix, and the depth of the positioning slots (531) is half the thickness of the rotating plate (530).

7. The three-dimensional cell culture and microenvironment simulation device according to claim 6, characterized in that: The bottom of the culture dish (600) is provided with four positioning blocks (610), which are respectively inserted into four positioning slots (531).

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