Cytokine quantitative adding device for umbilical cord mesenchymal stem cell culture

Abstract

The utility model discloses a kind of cytokine quantitative adding devices for umbilical cord mesenchymal stem cell culture, more specifically in the field of biomedical technology, including bottom plate, and the adding device body is installed in bottom plate top;Sterile bin is installed in the inner wall of limiting frame, and sterile bin is slid in the groove in the top of adding device body reservation;Sterile bin inner wall is provided with hydrophobic filter membrane;Adding device body top is hinged with top cover;With microliter peristaltic pump instead of manual pipettor, high-precision quantitative delivery is realized according to preset parameter, accurately match cell actual demand, avoid dosage deviation, rely on feed pipe, material conveying pipe constitutes closed conveying path, match sterile bin, circular tube impurity scraping function, prevent pollution introduced by operation exposure;While card block limiting groove is stably positioned to sterile bin, further guarantee conveying path accurate docking.Three synergies, both solve the precision problem of manual addition and pollution risk, also avoid the problem of cell activity decline, batch quality difference is big.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, and more specifically, to a device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture. Background Technology

[0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells originating from the mesoderm, characterized by strong proliferative capacity, high differentiation potential, low immunogenicity, and non-tumorigenicity. Based on these superior properties, MSCs are widely used in regenerative medicine and the broader health industry. MSCs exert their effects through two direct and indirect mechanisms: 1. Direct mechanisms: including homing, directed and multi-lineage differentiation, and paracrine effects; 2. Indirect mechanisms: including bidirectional regulation of immune and inflammatory responses, anti-oxidative stress, anti-fibrosis, anti-apoptosis, hematopoietic support, participation in or promotion of angiogenesis, activation of endogenous stem cell proliferation and differentiation, dedifferentiation of differentiated normal cells in diseased tissues and organs, and anti-aging and anti-scarring effects. These mechanisms have been confirmed by extensive basic research and clinical trials.

[0003] Existing publication number CN211170664U discloses a combined separation and extraction device for umbilical cord mesenchymal stem cells, including a base, a support frame, a filter, a peristaltic pump, a negative pressure pump, a guide tube, a control valve, a sampling tube, a drainage tube, and a control circuit. The support frame is a frame structure with its axis perpendicular to the horizontal plane, and the filter is embedded in the support frame and coaxially distributed with the support frame. The upper end of the filter is connected to the sampling tube through the peristaltic pump, and the lower end of the filter is connected to at least one drainage tube through the negative pressure pump. The peristaltic pump and the sampling tube, and the negative pressure pump and the drainage tube are all interconnected through the control valve. The control circuit is located on the outer side of the support frame and is electrically connected to the peristaltic pump, the negative pressure pump, and the control valve. On the one hand, it can efficiently, conveniently, and accurately meet the needs of umbilical cord blood sample collection, filtration, and separation; on the other hand, the separation and filtration operation has high efficiency, the separated stem cells have good biological activity, and it can effectively prevent contamination from external sources. In the process of realizing this utility model, the inventors discovered the following problems with the existing technology: Existing cytokine quantitative addition devices for umbilical cord mesenchymal stem cell culture rely heavily on manual pipetting in traditional culture. This not only makes it difficult to achieve high-precision control but also increases the risk of contamination due to operational exposure, leading to a mismatch between the added amount and the actual needs of the cells. This can result in problems such as decreased cell viability and large batch-to-batch quality differences. Therefore, a device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture is proposed to address the above problems. Utility Model Content

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides a device for quantitative addition of cytokines for umbilical cord mesenchymal stem cell culture, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture, comprising a base plate, with an adding device body mounted on the top of the base plate; a limiting frame is fixedly connected to the top of the adding device body, and the limiting frame wraps around a pre-reserved groove on the top of the adding device body; a sterile chamber is installed on the inner side wall of the limiting frame, and the sterile chamber slides within the pre-reserved groove on the top of the adding device body; a hydrophobic filter membrane is provided on the inner side wall of the sterile chamber; limiting components are provided on both sides of the sterile chamber; a top cover is hinged to the top of the adding device body, and the top cover fits against the surface of the limiting frame when closed.

[0006] Preferably, the limiting component includes a locking block; the top of the limiting frame has a pair of limiting grooves corresponding to the position of the locking block; the locking block slides in the middle of the limiting groove.

[0007] Preferably, a micro-lift peristaltic pump is installed on the top of the top cover; the micro-lift peristaltic pump is provided with a feed pipe and a delivery pipe at both ends; the delivery pipe passes through the reserved hole at the top of the top cover and reaches the middle position of the hydrophobic filter membrane.

[0008] Preferably, a one-way anti-backflow valve core is provided near the end of the feed pipe; the one-way anti-backflow valve core is located in the middle of the hydrophobic filter membrane.

[0009] Preferably, the sterile chamber is equipped with a sealing cover on top; the sealing cover is provided with a circular tube on top, and the feed pipe passes through the middle of the circular tube to the middle of the hydrophobic filter membrane.

[0010] Preferably, a plug is fixedly connected to one side of the top cover; a slot is fixedly connected to the surface of the limiting frame, and the plug slides into the middle of the slot when the top cover is closed.

[0011] Preferably, a plurality of first magnetic blocks are fixedly attached to the surface of the insert block; a plurality of second magnetic blocks are fixedly attached to the inner sidewall of the first magnetic blocks, and the first magnetic blocks and the second magnetic blocks attract each other.

[0012] The technical effects and advantages of this utility model are as follows: Compared to existing technologies, this device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture replaces manual pipettes with a micro-pump, achieving high-precision quantitative delivery according to preset parameters. This precisely matches the actual needs of the cells, avoiding dosage deviations. A closed delivery path is formed by the feed and delivery tubes, coupled with a sterile chamber and a circular tube for impurity scraping, eliminating contamination introduced during operation. Simultaneously, the locking block and limiting groove ensure stable positioning of the sterile chamber, further guaranteeing precise alignment of the delivery path. These three features work synergistically to solve the accuracy problems and contamination risks of manual addition, while also avoiding issues such as decreased cell viability and significant batch-to-batch quality variations.

[0013] Compared with existing technologies, this device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture features a top cover, insert blocks, and a first magnetic block. During operation, the top cover works in conjunction with the insert blocks and the first magnetic block to achieve sealing and fixation. When the top cover is closed, the insert block fixed to one side slides into the slot on the surface of the limiting frame. At the same time, the first magnetic block on the surface of the insert block and the second magnetic block on the inner wall of the slot attract each other due to their opposite magnetic poles. This design, through the dual action of mechanical fitting and magnetic attraction, ensures that the top cover fits tightly against the limiting frame, sealing the sterile chamber within the preset space. The top cover can be quickly and securely closed without tools, improving operational efficiency. The dual fixation ensures that the top cover is not easily displaced, preventing contamination caused by seal failure of the sterile chamber. Attached Figure Description

[0014] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0015] Figure 2 This is a schematic diagram of the micro-lift peristaltic pump of this utility model.

[0016] Figure 3 This is a schematic diagram of the sealing cap of this utility model.

[0017] Figure 4 This is a schematic diagram of the insert block of this utility model.

[0018] The attached figures are labeled as follows: 1. Base plate; 11. Adding device body; 12. Limiting frame; 13. Sterile chamber; 14. Hydrophobic filter membrane; 15. Limiting component; 16. Top cover; 2. Locking block; 21. Limiting groove; 3. Micro-lift peristaltic pump; 31. Feed pipe; 32. Conveying pipe; 4. One-way anti-backflow valve core; 5. Sealing cover; 51. Circular tube; 6. Insert block; 61. Slot; 7. First magnetic block; 71. Second magnetic block. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model. Example 1

[0020] As attached Figures 1 to 4 The device shown is a quantitative cytokine addition device for umbilical cord mesenchymal stem cell culture, comprising a base plate 1, an addition device body 11 mounted on the top of the base plate 1; a limiting frame 12 fixedly connected to the top of the addition device body 11, and the limiting frame 12 covering a pre-reserved groove on the top of the addition device body 11; a sterile chamber 13 installed on the inner side wall of the limiting frame 12, and the sterile chamber 13 sliding within the pre-reserved groove on the top of the addition device body 11; a hydrophobic filter membrane 14 provided on the inner side wall of the sterile chamber 13; limiting components 15 provided on both sides of the sterile chamber 13; and a top cover 16 hinged to the top of the addition device body 11, and the top cover 16 fitting against the surface of the limiting frame 12 when closed.

[0021] In operation, the base plate 1 provides stable support for the main body 11 of the addition device, forming the foundation of the overall structure of the device. The main body 11 of the addition device serves as the main support for the limiting frame 12, which provides precise installation positioning for the sterile chamber 13 through its own reserved groove. At the same time, the limiting component 15 cooperates with the limiting frame 12 to limit and fix the sterile chamber 13 after installation, preventing the sterile chamber 13 from shifting during operation. After the sterile chamber 13 is fixed, the top cover 16 is closed to fit against the surface of the limiting frame 12, thus sealing the sterile chamber 13 in a clean space. The hydrophobic filter membrane 14 plays a role in this closed space, both blocking external microorganisms from entering to maintain sterility and achieving a balanced environment for internal and external gas exchange. Example 2

[0022] Based on Example 1, the solution in Example 1 will be further described in detail below with reference to the specific working method, such as... Figures 1 to 4 As shown below, see details: In a preferred embodiment, the limiting component 15 includes a locking block 2; after the aseptic chamber 13 is installed in the pre-reserved groove at the top of the base plate 1, the locking block 2 can slide along the groove of the limiting groove 21; a pair of limiting grooves 21 are opened at the top of the limiting frame 12, and the positions of the locking block 2 are corresponding; by adjusting the position of the locking block 2 in the limiting groove 21, the edge of the aseptic chamber 13 is pressed and limited; the locking block 2 slides in the middle of the limiting groove 21; this setting can prevent the aseptic chamber 13 from shifting or shaking during the subsequent docking of the feed tube 32 and the addition of cytokines, ensuring that the aseptic chamber 13 is always in the preset accurate position.

[0023] In a preferred embodiment, a micro-lift peristaltic pump 3 is installed on the top of the top cover 16; after the micro-lift peristaltic pump 3 is started, its internal precision power component drives the formation of negative pressure; the micro-lift peristaltic pump 3 is provided with a feed pipe 31 and a delivery pipe 32 at both ends; the solution is drawn from the cytokine storage source through the feed pipe 31; the delivery pipe 32 passes through the reserved hole at the top of the top cover 16 and reaches the middle position of the hydrophobic filter membrane 14; wherein the micro-lift peristaltic pump 3 adjusts the delivery accuracy and flow rate according to preset parameters, and delivers the solution directionally to the direction of the sterile chamber 13 through the delivery pipe 32, and the delivery pipe 32 precisely connects with the circular tube 51 and the hydrophobic filter membrane 14 during delivery to ensure the continuity of the liquid path.

[0024] In a preferred embodiment, a one-way anti-backflow valve core 4 is provided near the end of the feed pipe 32. The one-way anti-backflow valve core 4 is installed near the end of the feed pipe 32 to form one-way flow control: the one-way anti-backflow valve core 4 is located in the middle of the hydrophobic filter membrane 14. When the micro-lift peristaltic pump 3 drives the cytokines to be transported along the feed pipe 32 towards the sterile chamber 13, the liquid pressure will push the valve core inside the one-way anti-backflow valve core 4 to open, allowing the cytokines to pass smoothly. If the micro-lift peristaltic pump 3 stops transporting or negative pressure occurs in the feed pipe 32, the valve core will automatically reset and close by its own elasticity or gravity, blocking the reverse flow path of the liquid.

[0025] In a preferred embodiment, a sealing cap 5 is installed on the top of the sterile chamber 13; when the feed tube 32 extends towards the sterile chamber 13, it needs to pass through the inside of the circular tube 51. The inner wall of the circular tube 51 will tightly fit the outer surface of the feed tube 32, and scrape off the impurities attached to its surface during the insertion of the feed tube 32; the sealing cap 5 is provided with a circular tube 51 on top, and the feed tube 32 passes through the middle of the circular tube 51 and reaches the middle position of the hydrophobic filter membrane 14; at the same time, the sealing cap 5 is sealed with the edge of the sterile chamber 13, further reinforcing the sterile space of the sterile chamber 13.

[0026] In a preferred embodiment, a plug 6 is fixedly connected to one side of the top cover 16; a slot 61 is fixedly connected to the surface of the limiting frame 12, and when the top cover 16 is closed, the plug 6 slides into the middle of the slot 61; when the top cover 16 is closed so that it fits against the limiting frame 12, the plug 6 slides into the slot along the groove of the slot 61 to form a mechanical fit.

[0027] In a preferred embodiment, a plurality of first magnetic blocks 7 are fixedly attached to the surface of the insert 6; when the insert 6 slides into the slot 61, the first magnetic blocks 7 and the second magnetic blocks 71 are automatically attracted by magnetic attraction; a plurality of second magnetic blocks 71 are fixedly attached to the inner sidewall of the first magnetic blocks 7, and the first magnetic blocks 7 and the second magnetic blocks 71 attract each other; this arrangement can form a double fixation with the mechanical fitting of the insert 6 and the slot 61, firmly locking the top cover 16 in the position of fitting the limiting frame 12, and preventing the top cover 16 from shifting due to vibration or operation.

[0028] In this embodiment, the micro-peristaltic pump 3, hydrophobic filter membrane 14, etc. are all commercially available devices known to those skilled in the art. They can be customized or selected according to actual needs. Here, we are only using them and have not made any structural or functional improvements. We will not go into details here.

[0029] The working process of this utility model is as follows: First, before adding cytokines, the sterile chamber 13 is installed in the reserved slot at the top of the base plate 1. At this time, the locking block 2 in the limiting component 15 cooperates with the limiting groove 21 on the limiting frame 12. The locking block 2 can slide in the limiting groove 21, thereby limiting and fixing the position of the sterile chamber 13, ensuring that the sterile chamber 13 remains stable during the subsequent cytokines addition process, and providing a basis for sterile operation.

[0030] Then, the top cover 16 is closed, and the insert 6 on one side of the top cover 16 slides into the slot 61 on the surface of the limiting frame 12. The first magnetic block 7 on the surface of the insert 6 and the second magnetic block 71 on the inner side wall of the slot 61 attract each other, further reinforcing the connection between the top cover 16 and the limiting frame 12. Then, the micro-lift peristaltic pump 3 is started, and the cytokines enter the micro-lift peristaltic pump 3 through the feed pipe 31. The micro-lift peristaltic pump 3 precisely controls the delivery amount of cytokines according to preset parameters. Then, the cytokines are delivered through the delivery pipe 32. The one-way anti-backflow valve core 4 on the delivery pipe 32 can prevent the cytokines from flowing back. The device uses a reflux mechanism to ensure accurate quantitative delivery. When the feed tube 32 enters the middle of the hydrophobic filter membrane 14, the circular tube 51 adheres to the surface of the feed tube 32, removing impurities from its surface. Subsequently, the cytokines enter the umbilical cord mesenchymal stem cell culture system inside the sterile chamber 13 through the circular tube 51 at the top of the sealing cap 5. At the same time, the hydrophobic filter membrane 14 on the inner wall of the sterile chamber 13 can achieve gas exchange while maintaining sterility, ensuring the stability of the stem cell culture process. The above describes the working principle of this cytokine quantitative addition device for umbilical cord mesenchymal stem cell culture.

Claims

1. A device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture, comprising a base plate (1), characterized in that: The bottom plate (1) is equipped with an addition device body (11) on top; a limiting frame (12) is fixedly connected to the top of the addition device body (11), and the limiting frame (12) is wrapped around the pre-reserved groove on the top of the addition device body (11); a sterile chamber (13) is installed on the inner side wall of the limiting frame (12), and the sterile chamber (13) slides in the pre-reserved groove on the top of the addition device body (11); a hydrophobic filter membrane (14) is provided on the inner side wall of the sterile chamber (13); limiting components (15) are provided on both sides of the sterile chamber (13); a top cover (16) is hinged to the top of the addition device body (11), and the top cover (16) is in contact with the surface of the limiting frame (12) when closed.

2. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 1, characterized in that: The limiting component (15) includes a locking block (2); the top of the limiting frame (12) is provided with a pair of limiting grooves (21) and they correspond to the position of the locking block (2); the locking block (2) slides in the middle of the limiting groove (21).

3. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 2, characterized in that: A micro-lift peristaltic pump (3) is installed on the top of the top cover (16); the micro-lift peristaltic pump (3) is provided with a feed pipe (31) and a delivery pipe (32) at both ends; the delivery pipe (32) passes through the reserved hole at the top of the top cover (16) and reaches the middle position of the hydrophobic filter membrane (14).

4. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 3, characterized in that: The feed pipe (32) is provided with a one-way anti-backflow valve core (4) near its end; the one-way anti-backflow valve core (4) is located in the middle of the hydrophobic filter membrane (14).

5. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 1, characterized in that: The sterile chamber (13) is equipped with a sealing cover (5) on top; a circular tube (51) is provided on the top of the sealing cover (5), and the feed pipe (32) passes through the middle of the circular tube (51) and reaches the middle of the hydrophobic filter membrane (14).

6. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 5, characterized in that: A plug (6) is fixedly connected to one side of the top cover (16); a slot (61) is fixedly connected to the surface of the limiting frame (12), and the plug (6) slides into the middle of the slot (61) when the top cover (16) is closed.

7. The device for quantitatively adding cytokines for umbilical cord mesenchymal stem cell culture according to claim 6, characterized in that: The insert (6) has a plurality of first magnetic blocks (7) fixedly attached to its surface; the first magnetic blocks (7) have a plurality of second magnetic blocks (71) fixedly attached to their inner sidewalls, and the first magnetic blocks (7) and the second magnetic blocks (71) attract each other.

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