Plant adventitious root growth culture device

Abstract

The utility model relates to a kind of plant adventitious root growth culture devices, belong to biological culture technical field, including: culture jar and gas even block, the top of culture jar is equipped with feed hole, discharge hole and gas outlet, its bottom is equipped with air inlet;Gas even block is sealingly inserted in air inlet and its bottom extends air inlet, the sidewall of gas even block that extends air inlet is equipped with gas supply hole and its top that extends into culture jar through air inlet is equipped with multiple interval distribution gas distribution hole, the inside of gas even block is equipped with with the gas supply channel that is communicated with gas supply hole and multiple gas distribution hole.The utility model inserts gas even block sealingly in air inlet, can utilize external gas supply device, through gas supply hole, gas supply channel and multiple gas distribution hole, provide evenly distributed oxygen to culture jar, increase the oxygen solubility of culture medium in culture jar, improve the growth efficiency of biological adventitious root.

Description

Technical Field

[0001] This utility model relates to the field of biological culture technology, and in particular to a plant adventitious root growth culture device. Background Technology

[0002] Adventitious roots are roots that develop on the stems or leaves of plants. In most cases, the occurrence of adventitious roots is due to injury to plant organs or stimulation by external factors such as hormones and pathogens. It is a regenerative response of the plant. The occurrence of adventitious roots expands the root system of the plant, giving the plant and cells the ability to regenerate. They are widely used in plant organ cuttings and tissue culture.

[0003] Currently, existing adventitious root culture devices often use air or oxygen supply pipes for air or oxygen transmission. However, these pipes only deliver air to a specific area, resulting in uneven oxygen distribution to the adventitious roots or low dissolved oxygen levels in the container, leading to poor culture results. Furthermore, another problem with adventitious root culture is microbial contamination, a significant factor limiting industrialization. No current culture device can effectively control microbial contamination, often relying on the addition of large amounts of antibiotics, resulting in severe antibiotic residues in the product and greatly impacting its quality and application.

[0004] Therefore, how to design a biological adventitious root growth culture device and culture method with uniform oxygen distribution and control of microbial contamination is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0005] This invention provides a plant adventitious root growth and culture device, which solves the technical problems of uneven oxygen distribution and microbial contamination in existing biological adventitious root culture devices.

[0006] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: a plant adventitious root growth and cultivation device, comprising: a cultivation tank and a gas distribution block.

[0007] The culture tank contains a culture medium and has an inlet, outlet, and vent at its top and an vent at its bottom. A gas distribution block is sealed and inserted into the vent, with its bottom extending out of the vent. The side wall of the gas distribution block extending out of the vent has a gas supply hole, and its top, which passes through the vent and enters the culture tank, has multiple spaced-apart gas distribution holes. The interior of the gas distribution block has a gas supply channel communicating with the gas supply hole and the multiple gas distribution holes, so as to provide uniformly distributed oxygen to the culture tank after an external gas supply device communicates with the gas supply hole.

[0008] The beneficial effects of this invention are: it improves the structure of traditional adventitious root growth and culture devices by sealing the gas distribution block inside the air inlet. Since the side wall of the gas distribution block extending out of the air inlet is provided with an air supply hole and the top of the block extending through the air inlet into the culture tank is provided with multiple air distribution holes, and since the interior of the gas distribution block is provided with an air supply channel that communicates with the air supply hole and multiple air distribution holes, an external air supply device (such as an air pump) can be used to provide uniformly distributed oxygen to the culture tank through the air supply hole, air supply channel and multiple air distribution holes, thereby increasing the dissolved oxygen content of the culture medium in the culture tank and improving the growth efficiency of adventitious roots.

[0009] Based on the above technical solution, the present invention can be further improved as follows.

[0010] Furthermore, the specific preparation method of the gas-distributed block is as follows: ① Add 5 wt.% diatomaceous earth (d50 = 11.3 μm) as a sintering aid, 4-6 wt.% carbon powder, polyethylene paraffin, and lignocellulose as a composite pore-forming agent, 1-2 wt.% carboxymethyl cellulose as a binder, and a specific amount of Ag-NPs as an additive to alumina powder (d50 = 150.0 μm); ② Mix the above powders evenly using a V-type mixer, then add 5 wt.% water to the mixed powder, and pressurize at 20 MPa. Under pressure, the mixed powder is dry-pressed into a disc with a diameter of 100 mm and a height of 20 mm; ③ After aging in air overnight, the green body is placed in a muffle furnace and heated to 630°C at a rate of 1°C / min, and held for 2 hours to remove organic additives; ⑤ Continue heating at a rate of 5°C / min to the predetermined sintering temperature (1410~1580°C) and hold for 6 hours, and the cooling method is furnace cooling; ⑥ The gas distribution block contains Ag-NPs, which can kill some Gram-negative and Gram-positive bacteria, further ensuring the purity of the gas in the culture tank.

[0011] Furthermore, it also includes an air outlet plug, an air outlet pipe, an air outlet filter valve, an air inlet pipe, and an air inlet filter valve. The air outlet plug is inserted into the air outlet hole and has a vent hole thereon. One end of the air outlet pipe is inserted into and connected to the vent hole. The air outlet filter valve is installed in the air outlet pipe. One end of the air inlet pipe is inserted into and connected to the air supply hole and its other end is connected to the external air supply device. The air inlet filter valve is installed in the air inlet pipe.

[0012] The further beneficial effects of adopting the above are: by installing an inlet filter valve and an outlet filter valve in the inlet pipe and outlet pipe respectively, it is possible to effectively prevent the backflow of polluted gas, avoid microbial contamination, improve the smoothness of exhaust of the culture tank, and prevent explosion caused by excessive pressure.

[0013] Furthermore, it also includes a feed cover and a discharge cover, wherein the feed cover is detachably installed at the feed hole; and the discharge cover is detachably installed at the discharge hole.

[0014] The further beneficial effect of adopting the above is that by using the inlet cover and outlet cover to be detachably installed at the inlet and outlet ports respectively, external dust or impurities can be effectively prevented from entering the culture tank.

[0015] Furthermore, it also includes a support ring and multiple support legs, wherein the support ring is detachably fitted onto the lower periphery of the culture tank; the top ends of the multiple support legs are fixed to the support ring at intervals.

[0016] The further beneficial effect of adopting the above is that: firstly, the support ring is detachably sleeved on the lower periphery of the culture vessel; then, multiple support legs are fixed to the support ring at intervals, which can ensure the stability of the culture vessel.

[0017] Furthermore, the diameter of the air distribution pore is 1–2 μm.

[0018] The further beneficial effect of adopting the above is that by designing the pore size of the stomata to be in the micrometer range, micrometer-sized bubbles can be generated in the stomata, increasing the dissolved oxygen content of the culture medium in the culture tank and improving the growth efficiency of adventitious roots.

[0019] In addition, a method for cultivating adventitious roots is provided, including the aforementioned adventitious root cultivation device, the specific steps of which are as follows:

[0020] S1. Add culture medium into the culture tank through the feed hole;

[0021] S2, the feed port, discharge port and air outlet of the sealed culture tank, and the air supply port of the uniformly distributed atmosphere block are also sealed.

[0022] S3. Place the culture tank and gas into the sterilizer in equal blocks for sterilization.

[0023] S4. After sterilization, remove the incubator and gas and divide them into equal pieces;

[0024] S5. After the culture medium in the incubator has cooled to room temperature, sterile adventitious roots are introduced through the feed inlet.

[0025] S6. Oxygen is evenly distributed into the culture tank through the air supply hole, air supply channel and multiple air distribution holes. At the same time, the air in the culture tank and the waste gas produced by the biological adventitious roots are discharged through the air outlet.

[0026] S7. Place the culture jar in the dark and culture the adventitious roots of the organism at 20-30℃. Attached Figure Description

[0027] Figure 1 This is a front view of the internal structure of a plant adventitious root growth and cultivation device according to the present invention;

[0028] Figure 2This is a top view of the internal structure of a plant adventitious root growth and cultivation device according to the present invention.

[0029] Figure 3 This invention relates to the gas distribution inside a culture tank in a plant adventitious root growth culture device.

[0030] The attached diagram lists the components represented by each number as follows:

[0031] 1. Culture tank; 11. Feed port; 12. Discharge port; 13. Air vent; 14. Air inlet; 2. Gas distribution block; 3. Air vent plug; 4. Air vent pipe; 5. Air vent filter valve; 6. Air inlet pipe; 7. Air inlet filter valve; 8. Feed cover; 9. Discharge cover; 10. Support ring; 15. Support leg. Detailed Implementation

[0032] The principles and features of this utility model are described below with reference to the accompanying drawings. The examples given are only for explaining this utility model and are not intended to limit the scope of this utility model.

[0033] like Figure 1 As shown, a plant adventitious root growth culture device includes: a culture tank 1 and a gas distribution block 2.

[0034] The culture tank 1 contains culture medium and has a feed hole 11, a discharge hole 12 and an air outlet 13 at its top and an air inlet 14 at its bottom. The gas distribution block 2 is sealed and inserted into the air inlet 14 and its bottom end extends out of the air inlet 14. The side wall of the gas distribution block 2 extending out of the air inlet 14 has an air supply hole and its top end, which extends through the air inlet 14 into the culture tank 1, has multiple spaced air distribution holes. The gas distribution block 2 has an air supply channel inside that communicates with the air supply hole and the multiple air distribution holes, so that after the external air supply device is connected to the air supply hole, it can provide uniformly distributed oxygen into the culture tank 1.

[0035] like Figure 1 and Figure 2 As shown, in some specific embodiments, it may also include an air outlet plug 3, an air outlet pipe 4, an air outlet filter valve 5, an air inlet pipe 6, and an air inlet filter valve 7. The air outlet plug 3 is inserted into the air outlet hole 13 and has a vent hole thereon; one end of the air outlet pipe 4 is inserted into and connected to the vent hole; the air outlet filter valve 5 is installed in the air outlet pipe 4; one end of the air inlet pipe 6 is inserted into and connected to the air supply hole and its other end is connected to an external air supply device; the air inlet filter valve 7 is installed in the air inlet pipe 6.

[0036] like Figure 1 As shown, in some specific embodiments, a feed cover 8 and a discharge cover 9 may also be included. The feed cover 8 is detachably installed at the feed hole 11; the discharge cover 9 is detachably installed at the discharge hole 12.

[0037] like Figure 1 As shown, in some specific embodiments, it may also include a support ring 10 and a plurality of support legs 15. The support ring 10 is detachably sleeved on the lower periphery of the culture tank 1; the top ends of the plurality of support legs 15 are fixed at intervals on the support ring 10.

[0038] Specifically, the diameter of the air distribution pores can be 1 to 2 μm.

[0039] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A plant adventitious root growth cultivation device, characterized in that, include: Culture tank (1), the culture tank (1) is filled with culture medium and has a feed hole (11), a discharge hole (12) and an air outlet (13) at its top and an air inlet (14) at its bottom. A gas equalization block (2) is sealed and inserted into the air inlet (14) with its bottom end extending out of the air inlet (14). The side wall of the gas equalization block (2) extending out of the air inlet (14) is provided with an air supply hole, and its top end, which extends through the air inlet (14) into the culture tank (1), is provided with multiple spaced air distribution holes. The interior of the gas equalization block (2) is provided with an air supply channel that communicates with the air supply hole and the multiple air distribution holes, so as to provide uniformly distributed oxygen to the culture tank (1) after the external air supply device communicates with the air supply hole.

2. The plant adventitious root growth culture apparatus according to claim 1, wherein It also includes an air outlet plug (3), an air outlet pipe (4), an air outlet filter valve (5), an air inlet pipe (6), and an air inlet filter valve (7). The air outlet plug (3) is inserted into the air outlet hole (13) and has a vent hole thereon. One end of the air outlet pipe (4) is inserted into and connected to the vent hole. The air outlet filter valve (5) is installed in the air outlet pipe (4). One end of the air inlet pipe (6) is inserted into and connected to the air supply hole and its other end is connected to the external air supply device. The air inlet filter valve (7) is installed in the air inlet pipe (6).

3. The plant adventitious root growth culture device according to claim 1, characterized in that, It also includes a feed cover (8) and a discharge cover (9), wherein the feed cover (8) is detachably installed at the feed hole (11); and the discharge cover (9) is detachably installed at the discharge hole (12).

4. The plant adventitious root growth culture device according to claim 1, characterized in that, It also includes a support ring (10) and a plurality of support legs (15), wherein the support ring (10) is detachably fitted on the lower periphery of the culture tank (1); the top ends of the plurality of support legs (15) are fixed at intervals on the support ring (10).

5. The apparatus according to claim 1, wherein The diameter of the air distribution pore is 1 to 2 μm.

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