A purification structure of a recombinant protein

Abstract

The utility model discloses a kind of purification structures of recombinant protein, including protein purifier, the lower end outer surface of protein purifier is fixedly installed with pedestal, the lower part of the outer surface of the front end of pedestal is equipped with embedded slot, one side of the pedestal is provided with liquid leakage collection structure, the liquid leakage collection structure includes collecting groove, handle, observation window, splash-proof plate and iron block, and one side of the collecting groove is inserted into embedded slot, the other side of the collecting groove is extended to the outside of embedded slot, the left and right sides of the rear end of embedded slot are fixedly installed with magnet. The utility model said a kind of purification structures of recombinant protein, one side of liquid leakage collection structure is inserted into embedded slot, collection effect is better, by fixedly installed with splash-proof plate in collecting groove, splash-proof plate is zigzag line board, when liquid drops in collecting groove, by the setting of splash-proof plate, play the role of splash-proof.

Description

Technical Field

[0001] This utility model relates to the technical field of recombinant protein processing equipment, specifically to a purification structure of a recombinant protein. Background Technology

[0002] Purification of recombinant proteins is a crucial step in the field of biotechnology, and protein purification instruments, as core equipment, are widely used in laboratories and industrial production. During the purification process, when the recombinant protein solution passes through a chromatography column or other separation media, leakage may occur due to improper operation or inadequate equipment sealing.

[0003] In existing technologies, a leakage tank is typically placed at the bottom of the front end of a protein purification instrument to collect leaked liquid, but this method has the following significant drawbacks: 1. Liquid splashing problem: When liquid drips from a height into the drain tank, it is easy to splash, causing the liquid to splash outside the tank, contaminating the experimental environment or equipment surface, increasing the difficulty of cleaning and the risk of cross-contamination; 2. Limited collection effect: Traditional leakage tanks are mostly simple open designs, lacking effective anti-splash structures, and the liquid flow path is single, which cannot effectively suppress splashing.

[0004] This has brought certain adverse effects to people's use. Therefore, we propose a purification structure for recombinant proteins. Utility Model Content

[0005] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a purification structure for recombinant proteins, which features a leak-proof and splash-proof structure and is easy to assemble, effectively solving the problems in the background technology.

[0006] (II) Technical Solution To achieve the above objectives, the technical solution adopted by this utility model is as follows: a recombinant protein purification structure, including a protein purification instrument, a base is fixedly installed on the lower outer surface of the protein purification instrument, an embedding groove is opened at the lower part of the front outer surface of the base, a leakage collection structure is provided on one side of the base, the leakage collection structure includes a collection groove, a handle, an observation window, a splash guard and an iron block, and one side of the collection groove is inserted into the embedding groove, the other side of the collection groove extends out of the embedding groove, and magnets are fixedly installed on the left and right sides of the rear end of the embedding groove.

[0007] Preferably, the iron block is fixedly installed on the outer surface of the rear end of the collection tank, and the magnet and the iron block are magnetically connected.

[0008] Preferably, the handle is fixedly installed on the left and right ends of the front outer surface of the collection tank, and the observation window is located in the middle of the front outer surface of the collection tank.

[0009] Preferably, the number of anti-splash plates is multiple sets, and the multiple sets of anti-splash plates are fixedly installed at equal intervals inside the collection tank, and a liquid collection space is left between the bottom of the anti-splash plates and the bottom of the collection tank.

[0010] Preferably, adjacent anti-splash plates are staggered to form a non-linear liquid flow path.

[0011] Preferably, the surface of the anti-splash plate is covered with a hydrophobic coating.

[0012] Preferably, the hydrophobic coating is polytetrafluoroethylene (PTFE), and the thickness of the hydrophobic coating is 10~50μm.

[0013] (III) Beneficial Effects Compared with the prior art, this invention provides a purified structure of a recombinant protein, which has the following beneficial effects: 1. The purification structure of this recombinant protein, by setting multiple sets of serrated anti-splash plates in the collection tank and arranging them in a staggered manner to form a non-linear liquid flow path, can effectively buffer the impact force when the liquid drips and reduce liquid splashing. The surface of the anti-splash plate is covered with a hydrophobic coating of polytetrafluoroethylene (PTFE) (thickness 10~50μm), which can reduce liquid adhesion, further prevent splashing and promote the rapid collection of liquid into the collection space.

[0014] 2. The purification structure of this recombinant protein has a collection tank that is combined with the base through an embedded groove and fixed by magnetic attraction between a magnet and an iron block. This not only ensures a firm installation but also prevents displacement caused by equipment vibration, while facilitating quick disassembly and cleaning.

[0015] 3. The purification structure of this recombinant protein has an observation window at the front end of the collection tank, which can monitor the liquid accumulation in real time without frequently removing the tank, thus improving the operation efficiency. The bottom of the anti-splash plate and the collection tank retain a connected liquid collection space to ensure orderly liquid accumulation, avoid secondary splashing or retention, and improve collection efficiency. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the purified recombinant protein according to the present invention.

[0017] Figure 2 This is a partial structural diagram of the purified structure of a recombinant protein according to the present invention.

[0018] Figure 3 This is a schematic diagram of the leakage collection structure in the purification structure of a recombinant protein according to this invention.

[0019] Figure 4This is a side cross-sectional view of the leakage collection structure in the purification structure of a recombinant protein according to this invention.

[0020] In the diagram: 1. Protein purification instrument; 2. Base; 3. Leakage collection structure; 4. Embedding groove; 5. Magnet; 6. Collection groove; 7. Handle; 8. Observation window; 9. Anti-splash plate; 10. Iron block. Detailed Implementation

[0021] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0022] This example illustrates the purified structure of a recombinant protein.

[0023] like Figure 1-4 As shown, the apparatus includes a protein purification instrument 1. A base 2 is fixedly installed on the lower outer surface of the protein purification instrument 1. An embedding groove 4 is opened at the lower part of the front outer surface of the base 2. A leakage collection structure 3 is provided on one side of the base 2. The leakage collection structure 3 includes a collection groove 6, a handle 7, an observation window 8, a splash guard 9, and an iron block 10. One side of the collection groove 6 is inserted into the embedding groove 4, and the other side of the collection groove 6 extends out of the embedding groove 4. Magnets 5 are fixedly installed on the left and right sides of the rear end of the embedding groove 4.

[0024] Iron block 10 is fixedly installed on the outer rear end of collection tank 6, and magnet 5 is magnetically connected to iron block 10; handle 7 is fixedly installed on the left and right ends of the outer front end of collection tank 6, and observation window 8 is set in the middle of the outer front end of collection tank 6; there are multiple sets of anti-splash plates 9, which are fixedly installed at equal intervals inside collection tank 6, and a liquid collection space is left between the bottom of anti-splash plate 9 and the bottom of collection tank 6; adjacent anti-splash plates 9 are staggered to form a non-linear liquid flow path; the surface of anti-splash plate 9 is covered with a hydrophobic coating; the hydrophobic coating is polytetrafluoroethylene (PTFE), and the thickness of the hydrophobic coating is 10~50μm.

[0025] It should be noted that this utility model is a purification structure for recombinant proteins. The protein purification instrument 1 described in this article belongs to the prior art and can be effectively known to those skilled in the art. Specific details will not be elaborated further. The leakage collection structure 3, during installation, involves inserting one side of the collection tank 6 into the embedding groove 4, so that the iron block 10 and the magnet 5 are magnetically connected, improving the stability of the collection tank 6 after installation. One side of the collection tank 6 is inserted into the embedding groove 4, while the other side extends outside the embedding groove 4, facilitating better collection of dripping liquid. Anti-splash plates 9 are fixedly installed at equal intervals inside the collection tank 6. The anti-splash plates 9 are serrated lines; when liquid drips into the collection tank 6, the anti-splash plates 9 prevent splashing.

[0026] It should be noted that, in this document, relational terms such as first and second (number one, number two), etc., are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0027] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A purification structure of a recombinant protein, comprising a protein purification instrument (1), characterized in that: The lower outer surface of the protein purification instrument (1) is fixedly mounted with a base (2). The lower part of the front outer surface of the base (2) is provided with an embedding groove (4). A leakage collection structure (3) is provided on one side of the base (2). The leakage collection structure (3) includes a collection groove (6), a handle (7), an observation window (8), a splash guard (9), and an iron block (10). One side of the collection groove (6) is inserted into the embedding groove (4), and the other side of the collection groove (6) extends to the outside of the embedding groove (4). Magnets (5) are fixedly installed on the left and right sides of the rear end of the embedding groove (4).

2. The purification structure of a recombinant protein according to claim 1, characterized in that: The iron block (10) is fixedly installed on the outer surface of the rear end of the collection tank (6), and the magnet (5) is magnetically connected to the iron block (10).

3. The purification structure of a recombinant protein according to claim 1, characterized in that: The handle (7) is fixedly installed on the left and right ends of the front outer surface of the collection tank (6), and the observation window (8) is located in the middle of the front outer surface of the collection tank (6).

4. The purified structure of a recombinant protein according to claim 1, characterized in that: The number of anti-splash plates (9) is multiple sets, and the multiple sets of anti-splash plates (9) are fixedly installed at equal intervals inside the collection tank (6), and there is a liquid collection space between the bottom of the anti-splash plates (9) and the bottom of the collection tank (6).

5. The purified structure of a recombinant protein according to claim 1, characterized in that: The adjacent anti-splash plates (9) are staggered to form a non-linear liquid flow path.

6. The purification structure of a recombinant protein according to claim 5, wherein: The surface of the anti-splash plate (9) is covered with a hydrophobic coating.

7. The purified structure of a recombinant protein according to claim 6, characterized in that: The hydrophobic coating is made of polytetrafluoroethylene, and the thickness of the hydrophobic coating is 10~50μm.

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