Protein gel strip sample pretreatment apparatus

Abstract

The utility model discloses a protein glue strip sample pretreatment equipment relates to biological sample pretreatment technical field, and protein glue strip sample pretreatment equipment includes: first equipment body and second equipment body, and first equipment body places reagent bottle and waste liquid collection bottle, and second equipment body places reagent reaction tube, reagent extraction pump, reagent extraction pipe, waste liquid collection pump and waste liquid collection pipe, and reagent extraction pipe is connected with first needle, and reagent extraction pump is connected with reagent extraction pipe and is used for driving reagent extraction pipe to draw and send the reagent in reagent bottle to first needle and from first needle into reagent reaction tube, and waste liquid collection pipe is connected with second needle, and waste liquid collection pump is connected with waste liquid collection pipe and is used for driving waste liquid collection pipe to draw and send the waste liquid in reagent reaction tube to waste liquid collection bottle through second needle, the protein glue strip sample pretreatment equipment of the utility model embodiment can avoid reagent and waste liquid cross -contamination, promote the accuracy of experimental result, and can improve the convenience of operation.

Description

Technical Field

[0001] This utility model relates to the field of biological sample pretreatment technology, and in particular to a protein strip sample pretreatment device. Background Technology

[0002] In proteomics research, pretreatment of protein strips (such as gel bands separated by SDS-PAGE) is a crucial pretreatment step for mass spectrometry analysis. This mainly includes decolorization and dehydration, reduction and alkylation, washing and dehydration, and enzymatic digestion, involving multiple reagent additions and waste liquid recovery. Traditional pretreatment primarily relies on single-pump, single-channel equipment. In such equipment, a single pump and single needle must simultaneously perform both reagent addition and waste liquid recovery. To avoid cross-contamination, the tubing must be emptied before each operation, which is time-consuming and inefficient. Furthermore, using a single pump and single needle for both reagent addition and waste liquid recovery can lead to residual reagents contaminating subsequent samples and affecting the accuracy of experimental results, indicating room for improvement. Utility Model Content

[0003] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention proposes a protein strip sample pretreatment device that can separate the reagent dispensing and waste liquid recovery paths, effectively avoiding cross-contamination between reagents and waste liquid, improving the accuracy of experimental results, reducing the need for cleaning the tubing before each reagent dispensing, improving operational convenience and efficiency, and automating both reagent dispensing and waste liquid recovery, further enhancing operational convenience and efficiency.

[0004] The protein strip sample pretreatment device according to an embodiment of the present invention includes: a first device body and a second device body, wherein the first device body holds a reagent bottle and a waste liquid collection bottle, and the second device body holds a reagent reaction tube; a reagent extraction pump, a reagent extraction tube, a waste liquid collection pump, and a waste liquid collection tube; the reagent extraction tube is connected to a first needle, the reagent extraction pump is connected to the reagent extraction tube and is used to drive the reagent extraction tube to draw the reagent in the reagent bottle to the first needle and then into the reagent reaction tube from the first needle; the waste liquid collection tube is connected to a second needle, and the waste liquid collection pump is connected to the waste liquid collection tube and is used to drive the waste liquid collection tube to draw the waste liquid in the reagent reaction tube to the waste liquid collection bottle through the second needle.

[0005] According to the protein strip sample pretreatment device of this utility model embodiment, the reagent injection and waste liquid recovery can be realized separately by setting a reagent extraction tube and a waste liquid collection tube. The reagent injection and waste liquid recovery paths can be separated, effectively avoiding cross-contamination between reagents and waste liquid, improving the accuracy of experimental results, and reducing the need to clean the pipeline before each reagent injection, thus improving the convenience and efficiency of operation. Moreover, by setting a reagent extraction pump and a waste liquid collection pump, the reagent injection and waste liquid recovery can be automated, further improving the convenience and efficiency of operation.

[0006] According to some embodiments of the protein strip sample pretreatment device of the present invention, the reagent extraction pump and the waste liquid collection pump are both installed in the first device body, and the reagent extraction pump and the waste liquid collection pump are distributed separately in the first device body.

[0007] According to some embodiments of the protein strip sample pretreatment device of the present invention, the first device body includes a first mounting surface, a second mounting surface, and a connecting side surface. The first mounting surface and the second mounting surface are located on opposite sides of the first device body. The reagent extraction pump is mounted on the first mounting surface, and the waste liquid collection pump is mounted on the second mounting surface. The connecting side surface is connected between the first mounting surface and the second mounting surface. The connecting side surface is provided with a receiving groove, and the reagent bottle and the waste liquid collection bottle are both placed in the receiving groove.

[0008] According to some embodiments of the protein strip sample pretreatment device of the present invention, the reagent extraction pump is connected to a reagent extraction valve, the reagent extraction valve is provided with at least one first inlet and at least one first outlet; wherein, the reagent extraction tube includes a first inlet tube body and a first outlet tube body, one end of the first inlet tube body is connected to the first inlet and the other end is adapted to extend into the reagent bottle, one end of the first outlet tube body is connected to the first outlet and the other end is provided with the first needle.

[0009] According to some embodiments of the protein strip sample pretreatment device of the present invention, the waste liquid collection pump is connected to a waste liquid collection valve, the waste liquid collection valve is provided with at least one second inlet and at least one second outlet; wherein, the waste liquid collection tube includes a second inlet tube body and a second outlet tube body, one end of the second inlet tube body is connected to the second needle and the other end is connected to the second inlet, one end of the second outlet tube body is connected to the second outlet and the other end is adapted to extend into the waste liquid collection bottle.

[0010] The protein strip sample pretreatment device according to some embodiments of the present invention further includes a pump control module. The reagent extraction pump and the waste liquid collection pump are both electrically connected to the pump control module, and the pump control module is used to independently control the reagent extraction pump and the waste liquid collection pump to operate selectively.

[0011] According to some embodiments of the protein strip sample pretreatment device of the present invention, the second device body is equipped with a mounting frame, and both the first needle and the second needle can be movably mounted on the mounting frame, and both can selectively move in three degrees of freedom in the X, Y and Z directions above the reagent reaction tube.

[0012] According to some embodiments of the protein strip sample pretreatment device of the present invention, the mounting frame is provided with a first mounting seat, the position of the first mounting seat relative to the mounting frame is adjustable, and the first needle is detachably mounted on the first mounting seat; and / or, the mounting frame is provided with a second mounting seat, the position of the second mounting seat relative to the mounting frame is adjustable, and the second needle is detachably mounted on the second mounting seat.

[0013] According to some embodiments of the protein strip sample pretreatment device of the present invention, the first mounting base is provided with a first snap-fit ​​part and / or a first magnetic element, the first needle is snap-fitted with the first snap-fit ​​part, and the first needle is adapted to be magnetically fixed to the first magnetic element; and / or, the second mounting base is provided with a second snap-fit ​​part and / or a second magnetic element, the second needle is snap-fitted with the second snap-fit ​​part, and the second needle is adapted to be magnetically fixed to the second magnetic element.

[0014] According to some embodiments of the protein strip sample pretreatment device of the present invention, the first device body and the second device body are distributed separately, and the reagent extraction tube and the waste liquid collection tube are at least partially constructed as flexible tubes.

[0015] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0016] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which: Figure 1 This is a schematic diagram of the protein gel strip sample pretreatment device according to an embodiment of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the protein gel strip sample pretreatment device according to an embodiment of the present invention. Figure 2 ; Figure 3 This is a schematic diagram of the structure of the first device body according to an embodiment of the present utility model. Figure 1 ; Figure 4 This is a schematic diagram of the structure of the first device body according to an embodiment of the present utility model. Figure 2 ; Figure 5 This is a schematic diagram of the structure of the first device body according to an embodiment of the present utility model. Figure 3 ; Figure 6 This is a schematic diagram of the structure of the first device body according to an embodiment of the present utility model. Figure 4 ; Figure 7 This is a schematic diagram of the structure of the second device body according to an embodiment of the present utility model. Figure 1 ; Figure 8 This is a schematic diagram of the structure of the second device body according to an embodiment of the present utility model. Figure 2 ; Figure 9 This is a schematic diagram of the structure of the second device body according to an embodiment of the present utility model. Figure 3 ; Figure 10 This is a partial schematic diagram of the second device body according to an embodiment of the present utility model.

[0017] Figure label: Protein strip sample pretreatment equipment 100 First device body 1, reagent bottle 11, waste liquid collection bottle 12, first mounting surface 13, second mounting surface 14, connecting side 15, receiving tank 151. The second device body 2, reagent reaction tube 21, mounting bracket 22, first mounting base 221, first snap-fit ​​part 2211, first magnetic component 2212, first slider 2213, second sliding guide groove 2214, first sliding assembly 2215, second mounting base 222, second snap-fit ​​part 2221, second magnetic component 2222, third sliding guide groove 2223, second sliding assembly 2224, first sliding guide groove 223, slide rail 23, and insertion slot 24. Reagent extraction pump 3, reagent extraction valve 31, first inlet 311, Reagent extraction tube 4, first inlet tube 41, first outlet tube 42, first needle 421, first mating part 4211. Waste liquid collection pump 5, waste liquid collection valve 51, second outlet 512, Waste liquid collection tube 6, second inlet tube 61, second needle 611, second outlet tube 62. Detailed Implementation

[0018] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0019] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," etc., indicating the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, features defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more. In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0020] The following is for reference. Figures 1-10 The protein strip sample pretreatment device 100 according to an embodiment of the present invention can separate the reagent dispensing and waste liquid recycling paths, effectively avoiding cross-contamination between reagents and waste liquid, improving the accuracy of experimental results, and reducing the need to clean the pipeline before each reagent dispensing, thus improving the convenience and efficiency of operation. Moreover, it can automate the reagent dispensing and waste liquid recycling processes separately, further improving the convenience and efficiency of operation.

[0021] like Figures 1-10 As shown, a protein strip sample pretreatment device 100 according to an embodiment of the present invention includes: a first device body 1, a second device body 2, a reagent extraction pump 3, a reagent extraction tube 4, a waste liquid collection pump 5, and a waste liquid collection tube 6.

[0022] The first device body 1 contains a reagent bottle 11 and a waste liquid collection bottle 12. The second device body 2 contains a reagent reaction tube 21. A reagent extraction tube 4 is connected to a first needle 421. A reagent extraction pump 3 is connected to the reagent extraction tube 4 and is used to drive the reagent extraction tube 4 to draw the reagent in the reagent bottle 11 to the first needle 421 and then into the reagent reaction tube 21 through the first needle 421. A waste liquid collection tube 6 is connected to a second needle 611. A waste liquid collection pump 5 is connected to the waste liquid collection tube 6 and is used to drive the waste liquid collection tube 6 to draw the waste liquid in the reagent reaction tube 21 to the waste liquid collection bottle 12 through the second needle 611.

[0023] Specifically, the protein strip sample pretreatment device 100 is used to perform pretreatment steps such as decolorization and dehydration, reduction and alkylation, and washing and dehydration on protein strip samples to ensure the accuracy of subsequent mass spectrometry analysis. The protein strip sample pretreatment device 100 includes a first device body 1 and a second device body 2. The first device body 1 is used to place a reagent bottle 11 and a waste liquid collection bottle 12. The second device body 2 is used to place a reagent reaction tube 21. The reagent bottle 11 is used to store reagents for the pretreatment steps of the protein strip sample. The reagent reaction tube 21 is used to provide a reaction space for the protein strip sample and reagents to perform the pretreatment steps on the protein strip sample inside it. The waste liquid collection bottle 12 is used to store the waste liquid of the protein strip sample during the pretreatment steps. The first device body 1 and the second device body 2 are two separate devices, that is, the reagent bottle 11 and the waste liquid collection bottle 12 are set separately from the reagent reaction tube 21 to avoid interference and ensure the reliable performance of the pretreatment steps.

[0024] When the reagent bottle 11 and the waste liquid collection bottle 12 are placed on the first device body 1, they can be fixed to prevent them from tipping over. When the reagent reaction tube 21 is placed on the second device body 2, it can be fixed to prevent it from tipping over, ensuring the reliable execution of the pretreatment step. Both the first device body 1 and the second device body 2 can be constructed as a frame structure, which facilitates the placement or removal of the reagent bottle 11, the waste liquid collection bottle 12 and the reagent reaction tube 21.

[0025] Furthermore, the protein strip sample pretreatment device 100 also includes a reagent extraction pump 3, a reagent extraction tube 4, a waste liquid collection pump 5, and a waste liquid collection tube 6. The reagent extraction tube 4 is connected to a first needle 421, and the reagent extraction pump 3 is connected to the reagent extraction tube 4 and is used to drive the reagent extraction tube 4 to draw the reagent from the reagent bottle 11 to the first needle 421 and then into the reagent reaction tube 21. One end of the reagent extraction tube 4 can be connected to the first needle 421, allowing the first needle 421 to extend into the reagent... Inside the reaction tube 21, the other end of the reagent extraction tube 4 is extended to the reagent bottle 11, thus connecting the reagent bottle 11 and the reagent reaction tube 21 through the reagent extraction tube 4. When the first needle 421 is extended into the reagent reaction tube 21, the reagent in the reagent bottle 11 can flow along the reagent extraction tube 4 and enter the reagent reaction tube 21 through the first needle 421, thereby realizing the addition of reagents. This allows the reagents to react with the protein strip sample in the reagent reaction tube 21, which is a pretreatment step for the protein strip sample.

[0026] Furthermore, the reagent extraction tube 4 is connected to the reagent extraction pump 3, which can be set at any position on the reagent extraction tube 4, so that the reagent extraction pump 3 can provide power to the reagent extraction tube 4, ensuring the reliability of the reagent extraction tube 4 in pumping the reagent from the reagent bottle 11 to the reagent reaction tube 21. Moreover, by setting the reagent extraction pump 3, the reagent dispensing can be automated, which can improve the convenience and efficiency of operation.

[0027] Meanwhile, the waste liquid collection tube 6 is connected to a second needle 611, and the waste liquid collection pump 5 is connected to the waste liquid collection tube 6 and is used to drive the waste liquid collection tube 6 to pump the waste liquid in the reagent reaction tube 21 to the waste liquid collection bottle 12 through the second needle 611. The second needle 611 can be connected to one end of the waste liquid collection tube 6 and can be extended into the reagent reaction tube 21. The other end of the waste liquid collection tube 6 can be extended into the waste liquid collection bottle 12, so that the reagent reaction tube 21 and the waste liquid collection bottle 12 can be connected through the waste liquid collection tube 6. Thus, when the second needle 611 is extended into the reagent reaction tube 21, the waste liquid of the protein strip sample during the pretreatment step in the reagent reaction tube 21 can pass through the second needle 611 and flow along the waste liquid collection tube 6 to enter the waste liquid collection bottle 12, thereby realizing the recovery of waste liquid.

[0028] Furthermore, the waste liquid collection pipe 6 is connected to a waste liquid collection pump 5, which can be positioned at any point on the waste liquid collection pipe 6. This allows the waste liquid collection pump 5 to provide power to the waste liquid collection pipe 6, ensuring the reliability of the waste liquid collection pipe 6 in pumping waste liquid from the reagent reaction tube 21 to the waste liquid collection bottle 12. By installing the waste liquid collection pump 5, waste liquid recycling can be automated, improving operational convenience and efficiency. Both the reagent extraction pump 3 and the waste liquid collection pump 5 can be syringe pumps.

[0029] According to the protein strip sample pretreatment device 100 of this utility model embodiment, the reagent injection tube 4 and waste liquid collection tube 6 can be set to realize the reagent injection and waste liquid recovery respectively, and the reagent injection and waste liquid recovery paths can be separated to effectively avoid cross-contamination between reagents and waste liquid, improve the accuracy of experimental results, and reduce the need to clean the pipeline before each reagent injection, thereby improving the convenience and efficiency of operation. Moreover, by setting the reagent injection pump 3 and waste liquid collection pump 5, the reagent injection and waste liquid recovery can be automated respectively, further improving the convenience and efficiency of operation.

[0030] In some embodiments, both the reagent extraction pump 3 and the waste liquid collection pump 5 are installed in the first device body 1, and the reagent extraction pump 3 and the waste liquid collection pump 5 are spaced apart and distributed in the first device body 1.

[0031] Specifically, the reagent extraction pump 3 is used to automate reagent dispensing, and the waste liquid collection pump 5 is used to automate waste liquid recycling. Both the reagent extraction pump 3 and the waste liquid collection pump 5 are installed on the first equipment body 1, meaning that the first equipment body 1 can simultaneously provide installation space for both the reagent extraction pump 3 and the waste liquid collection pump 5, enabling reliable installation and fixation of the reagent extraction pump 3 and the waste liquid collection pump 5, ensuring their reliable operation. Furthermore, the reagent extraction pump 3 and the waste liquid collection pump 5 are spaced apart on the first equipment body 1, which facilitates their connection to the reagent extraction pipe 4 and the waste liquid collection pipe 6 respectively, and avoids interference between them, thereby improving the operational reliability of each reagent extraction pump 3 and the waste liquid collection pump 5.

[0032] In some embodiments, the first device body 1 includes a first mounting surface 13, a second mounting surface 14, and a connecting side surface 15. The first mounting surface 13 and the second mounting surface 14 are located on opposite sides of the first device body 1. The reagent extraction pump 3 is mounted on the first mounting surface 13, and the waste liquid collection pump 5 is mounted on the second mounting surface 14. The connecting side surface 15 is connected between the first mounting surface 13 and the second mounting surface 14. The connecting side surface 15 is provided with a receiving groove 151, in which the reagent bottle 11 and the waste liquid collection bottle 12 are placed.

[0033] Specifically, the reagent extraction pump 3 and the waste liquid collection pump 5 are spaced apart and disposed on the first device body 1. The first device body 1 includes a first mounting surface 13 and a second mounting surface 14. The reagent extraction pump 3 is installed on the first mounting surface 13, that is, the first mounting surface 13 can provide an installation position for the reagent extraction pump 3. The waste liquid collection pump 5 is installed on the second mounting surface 14, that is, the second mounting surface 14 can provide an installation position for the waste liquid collection pump 5. The first mounting surface 13 and the second mounting surface 14 are located on opposite sides of the first device body 1. For example, the first mounting surface 13 and the second mounting surface 14 can be located on the front and rear sides or the left and right sides of the first device body 1, so that the first mounting surface 13 and the second mounting surface 14 can be spaced apart on the first device body 1. This allows the reagent extraction pump 3 and the waste liquid collection pump 5 to be spaced apart on the first device body 1, thereby improving the working reliability of each of the reagent extraction pump 3 and the waste liquid collection pump 5.

[0034] The first device body 1 also includes a connecting side 15, which has a receiving groove 151 for accommodating reagent bottles 11 and waste liquid collection bottles 12. The receiving groove 151 has a large space, providing sufficient room for both reagent bottles 11 and waste liquid collection bottles 12 to be placed simultaneously. Furthermore, the reagent bottles 11 and waste liquid collection bottles 12 can be spaced apart within the receiving groove 151 to avoid collisions or interference that could cause tipping. The connecting side 15 is connected to the first... The placement of the receiving tank 151 between the first mounting surface 13 and the second mounting surface 14 allows for a closer distance between both surfaces. This facilitates reducing the distance between the reagent bottle 11 and the reagent extraction pump 3, shortening the length of the reagent extraction tube 4 between the reagent bottle 11 and the reagent extraction pump 3, and reducing installation costs. Furthermore, it facilitates reducing the distance between the waste liquid collection bottle 12 and the waste liquid collection pump 5, shortening the length of the waste liquid collection tube 6 between the waste liquid collection bottle 12 and the waste liquid collection pump 5, and further reducing installation costs.

[0035] In such Figures 1-6 In the embodiment shown, the first mounting surface 13 is the left side of the first device body 1, and the second mounting surface 14 is the right side of the first device body 1. This allows the reagent extraction pump 3 and the waste liquid collection pump 5 to be set apart to ensure the reliability of their respective operations. The connecting side 15 is connected between the first mounting surface 13 and the second mounting surface 14 and is the bottom surface of the first device body 1, which facilitates the setting of the reagent bottle 11 and the waste liquid collection bottle 12.

[0036] It should be noted that, as Figure 1 , Figure 7 and Figure 10As shown, the reagent reaction tube 21 is placed on the second device body 2. An insertion slot 24 is provided at the bottom of the second device body 2. The insertion slot 24 is used to set the reagent reaction tube 21. There are multiple insertion slots 24, and multiple reagent reaction tubes 21 can be selectively set in multiple insertion slots 24 to fix the reagent reaction tube 21 and prevent the reagent reaction tube 21 from tipping over.

[0037] In some embodiments, the reagent extraction pump 3 is connected to a reagent extraction valve 31, which is provided with at least one first inlet 311 and at least one first outlet; wherein, the reagent extraction tube 4 includes a first inlet tube body 41 and a first outlet tube body 42, one end of the first inlet tube body 41 is connected to the first inlet 311 and the other end is adapted to extend into the reagent bottle 11, and one end of the first outlet tube body 42 is connected to the first outlet and the other end is provided with a first needle 421.

[0038] Specifically, the reagent extraction pump 3 is connected to the reagent extraction tube 4 via the reagent extraction valve 31. The reagent extraction valve 31 is provided with a first inlet 311 and a first outlet. The first inlet 311 is connected to the inlet end of the reagent extraction valve 31, allowing the reagent to enter the reagent extraction valve 31. The first outlet is connected to the outlet end of the reagent extraction valve 31, allowing the reagent to flow out of the reagent extraction valve 31. The first inlet 311 is set to at least one, that is, there can be one, two, three or more first inlets 311. At least one first inlet 311 can be connected to the reagent extraction valve 31, allowing the reagent to enter the reagent extraction valve 31 from any one of the first inlets 311. At the same time, the first outlet is set to at least one, that is, there can be one, two, three or more first outlets. At least one first outlet can be connected to the reagent extraction valve 31, allowing the reagent to flow out of the reagent extraction valve 31 from any one of the first outlets.

[0039] Meanwhile, the reagent extraction tube 4 includes a first inlet tube 41 and a first outlet tube 42. One end of the first inlet tube 41 is connected to the first inlet port 311 and the other end extends into the reagent bottle 11, thus connecting the first inlet tube 41 between the reagent bottle 11 and the first inlet port 311. This allows the reagent in the reagent bottle 11 to flow from the reagent bottle 11 along the first inlet tube 41 and through the first inlet port 311 into the reagent extraction valve 31. One end of the first outlet tube 42 is connected to the first outlet port and the other end is equipped with a first needle 421, thus connecting the first outlet tube 42 between the first outlet port and the first needle 421. When the first needle 421 is extended into the reagent reaction tube 21, the reagent in the reagent extraction valve 31 can flow from the first outlet along the first outlet tube 42 and through the first needle 421 into the reagent reaction bottle, thereby realizing the addition of reagents.

[0040] The first liquid inlet 311 and the first liquid outlet can be one. The first liquid inlet tube 41 is connected between the reagent bottle 11 and the first liquid inlet 311, and the first liquid outlet tube 42 is connected between the first liquid outlet and the reagent reaction tube 21. When the first needle 421 is inserted into the reagent reaction tube 21, the reagent bottle 11 can be connected to the reagent reaction tube 21, and the reagent can be added from the reagent bottle 11 to the reagent reaction tube 21.

[0041] Alternatively, multiple first inlet ports 311 and one first outlet port can be used, and the multiple first inlet ports 311 and the first outlet port can be selectively connected. Correspondingly, multiple first inlet tubes 41 and one first outlet tube 42 can be used, and the multiple first inlet tubes 41 can be connected between multiple reagent bottles 11 and multiple first inlet ports 311 respectively. The first outlet tube 42 can be connected between the first outlet port and the first needle 421. When the first needle 421 is inserted into the reagent reaction tube 21, the multiple reagent bottles 11 and the reagent reaction tube 21 can be selectively connected, and the reagent in one of the reagent bottles 11 can be selectively added to the reagent reaction tube 21.

[0042] Alternatively, there can be multiple first inlet ports 311 and multiple first outlet ports, with each of the multiple first inlet ports 311 connected to a corresponding number of first outlet ports. Correspondingly, there can be multiple first inlet tubes 41 and multiple first outlet tubes 42, with each of the multiple first inlet tubes 41 connected between a multiple reagent bottle 11 and a multiple first inlet port 311. The multiple first outlet ports are selectively connected to the first needle 421 through the multiple first outlet tubes 42. When the first needle 421 is inserted into the reagent reaction tube 21, the multiple reagent bottles 11 can be selectively connected to the reagent reaction tube 21, thereby selectively adding the reagent from one of the reagent bottles 11 to the reagent reaction tube 21.

[0043] In some embodiments, the waste liquid collection pump 5 is connected to a waste liquid collection valve 51, which is provided with at least one second inlet and at least one second outlet 512; wherein, the waste liquid collection pipe 6 includes a second inlet pipe body 61 and a second outlet pipe body 62, one end of the second inlet pipe body 61 is connected to a second needle 611 and the other end is connected to the second inlet, and one end of the second outlet pipe body 62 is connected to the second outlet 512 and the other end is adapted to extend into the waste liquid collection bottle 12.

[0044] Specifically, the waste liquid collection pump 5 is connected to the waste liquid collection pipe 6 via the waste liquid collection valve 51. The waste liquid collection valve 51 is provided with a second inlet and a second outlet 512. The second inlet is connected to the inlet end of the waste liquid collection valve 51, allowing waste liquid to enter the waste liquid collection valve 51. The second outlet 512 is connected to the outlet end of the waste liquid collection valve 51, allowing waste liquid to flow out of the waste liquid collection valve 51. The second inlet is set to at least one, that is, there can be one, two, three or more second inlets. At least one second inlet can be connected to the waste liquid collection valve 51, allowing waste liquid to enter the waste liquid collection valve 51 from any one of the second inlets. At the same time, the second outlet 512 is set to at least one, that is, there can be one, two, three or more second outlets. At least one second outlet 512 can be connected to the reagent extraction valve 31, allowing waste liquid to flow out of the waste liquid collection valve 51 from any one of the second outlets 512.

[0045] Meanwhile, the waste liquid collection tube 6 includes a second inlet tube 61 and a second outlet tube 62. One end of the second inlet tube 61 is connected to a second needle 611 and the other end is connected to a second inlet port, thus connecting the second inlet tube 61 to the second inlet port and the second needle 611. When the second needle 611 is extended into the reagent reaction tube 21, the waste liquid in the reagent reaction tube 21 can pass through the second needle 611 and flow along the second inlet tube 61 to enter the waste liquid collection valve 51. One end of the second outlet tube 62 is connected to the second outlet 512 and the other end extends into the waste liquid collection bottle 12, thus connecting the second outlet 62 to the second outlet 512 and the waste liquid collection bottle 12. This allows the waste liquid in the waste liquid collection valve 51 to flow from the second outlet 512 along the second outlet tube 62 and into the waste liquid collection bottle 12, thereby achieving waste liquid recycling.

[0046] The second liquid inlet and the second liquid outlet 512 can each be a single unit. The second liquid inlet tube 61 is connected between the reagent reaction tube 21 and the second liquid inlet, and the second liquid outlet tube 62 is connected between the second liquid outlet 512 and the waste liquid collection bottle 12. When the second needle 611 is inserted into the reagent reaction tube 21, the reagent reaction tube 21 can be connected to the waste liquid collection bottle 12, and the waste liquid can be drawn from the reagent reaction tube 21 to the waste liquid collection bottle 12.

[0047] Alternatively, there can be one second inlet and multiple second outlets 512. The multiple second outlets 512 can be selectively connected to the second inlet. Correspondingly, there can be one second inlet tube 61 and multiple second outlet tubes 62. The second inlet tube 61 is connected between the second needle 611 and the second inlet, and the multiple second outlet tubes 62 are respectively connected between the multiple second outlets 512 and the multiple waste liquid collection bottles 12. When the second needle 611 is inserted into the reagent reaction tube 21, the multiple waste liquid collection bottles 12 can be selectively connected to the reagent reaction tube 21, and the waste liquid in the reagent reaction tube 21 can be selectively pumped to one of the waste liquid collection bottles 12.

[0048] Alternatively, there can be multiple second inlets and multiple second outlets 512, with each inlet and outlet connected to the other. Correspondingly, there can be multiple second inlet tubes 61 and multiple outlet tubes 62. Multiple inlet tubes 61 can selectively connect the multiple inlets to the second needle 611, and multiple outlet tubes 62 can connect the multiple outlets 512 to the multiple waste collection bottles 12. When the second needle 611 is inserted into the reagent reaction tube 21, multiple waste collection bottles 12 can be selectively connected to the reagent reaction tube 21, thereby selectively pumping the waste liquid in the reagent reaction tube 21 to one of the waste collection bottles 12.

[0049] In some embodiments, the protein strip sample pretreatment device 100 further includes a pump control module, wherein the reagent extraction pump 3 and the waste liquid collection pump 5 are both electrically connected to the pump control module, and the pump control module is used to independently control the reagent extraction pump 3 and the waste liquid collection pump 5 to operate selectively.

[0050] Specifically, the pump control module is used to control the reagent extraction pump 3 and the waste liquid collection pump 5. Both the reagent extraction pump 3 and the waste liquid collection pump 5 are electrically connected to the pump control module, so that the pump control module can be used to control the start and stop of the reagent extraction pump 3 and the waste liquid collection pump 5, thereby realizing the automation of reagent dispensing and waste liquid recovery. The pump control module is also used to independently control the reagent extraction pump 3 and the waste liquid collection pump 5 to operate selectively. That is, the pump control module can control the start or stop of one of the reagent extraction pump 3 and the waste liquid collection pump 5 separately, so that reagent dispensing and waste liquid recovery can be carried out separately to avoid cross-contamination. Alternatively, the pump control module can control the reagent extraction pump 3 and the waste liquid collection pump 5 to start and stop simultaneously. In this case, the first needle 421 and the second needle 611 can be inserted into different reagent reaction tubes 21 respectively, so that reagent dispensing and waste liquid recovery can also be carried out separately to effectively avoid cross-contamination.

[0051] The pump control module can send individual commands to the reagent extraction pump 3 and the waste liquid collection pump 5, and can independently set the start speed, maximum speed, stop speed and slope of each pump. It can also perform motion control and status monitoring on the reagent extraction pump 3 and the waste liquid collection pump 5. At the same time, the reagent extraction pump 3 and the waste liquid collection pump 5 can achieve differentiated operation through independent communication channels, effectively avoiding cross-contamination and improving process efficiency.

[0052] In some embodiments, the second device body 2 is equipped with a mounting frame 22, and both the first needle 421 and the second needle 611 are movably mounted on the mounting frame 22, and both can selectively move in three degrees of freedom in the X, Y and Z directions above the reagent reaction tube 21.

[0053] Specifically, the reagent reaction tube 21 is placed in the second device body 2, the reagent extraction tube 4 is connected to the first needle 421, the first needle 421 is used to add reagent to the reagent reaction tube 21, and the waste liquid collection tube 6 is connected to the second needle 611, the second needle 611 is used to extract the waste liquid in the reagent reaction tube 21. Thus, both the first needle 421 and the second needle 611 can be installed in the second device body 2 to achieve reliable operation of the first needle 421 and the second needle 611.

[0054] The second device body 2 is equipped with a mounting frame 22, which is fixed to the second device body 2 and provides mounting positions for the first needle 421 and the second needle 611. The first needle 421 and the second needle 611 can be movably mounted on the mounting frame 22. Under the premise of mounting the first needle 421 and the second needle 611, both the first needle 421 and the second needle 611 can be movable relative to the mounting frame 22. This allows the first needle 421 and the second needle 611 to selectively move in three degrees of freedom in the X, Y, and Z directions above the reagent reaction tube 21. When the first needle 421 moves above the reagent reaction tube 21, reagent can be added to the reagent reaction tube 21. When the second needle 611 moves above the reagent reaction tube 21, waste liquid in the reagent reaction tube 21 can be extracted.

[0055] In such Figure 1 In the embodiment shown, the X direction can be left and right, the Y direction can be front and back, and the Z direction can be up and down. The reagent is added through the first needle 421, which can accurately control the amount of reagent added. The waste liquid is extracted through the second needle 611, which can completely extract the waste liquid.

[0056] In some embodiments, the mounting bracket 22 is provided with a first mounting seat 221, the position of the first mounting seat 221 relative to the mounting bracket 22 is adjustable, and the first needle 421 is detachably mounted on the first mounting seat 221; and / or, the mounting bracket 22 is provided with a second mounting seat 222, the position of the second mounting seat 222 relative to the mounting bracket 22 is adjustable, and the second needle 611 is detachably mounted on the second mounting seat 222.

[0057] Specifically, the first needle 421 is movably mounted on the mounting frame 22. The mounting frame 22 has a first mounting base 221 for mounting the first needle 421. The first mounting base 221 is configured to be adjustable in position relative to the mounting frame 22, allowing the position of the first needle 421 to be adjusted by changing the position of the first mounting base 221 on the mounting frame 22. This allows the first needle 421 to be selectively moved above the reagent reaction tube 21. The first needle 421 is detachably mounted on the first mounting base 221, facilitating connection or disconnection of the first needle 421. This allows for easy installation or removal of the first needle 421, enabling it to be replaced with a pipette tip, microsyringe, multichannel dispensing head, etc., to adapt to different pretreatment steps and volume requirements. Furthermore, it facilitates replacement of the first needle 421 when it is damaged or fails, reducing subsequent maintenance costs.

[0058] The position of the first mounting base 221 relative to the mounting bracket 22 is adjustable, such as... Figure 1 , Figure 2 , Figure 7 , Figure 8 and Figure 10 As shown, a first sliding guide groove 223 can be formed on the mounting bracket 22. The first sliding guide groove 223 is constructed to extend in the left and right direction, and the first mounting base 221 can form a first slider 2213. The first slider 2213 is slidably installed in the first sliding guide groove 223 to achieve sliding engagement. In this way, the position of the first mounting base 221 on the mounting bracket 22 can be changed by sliding, so that the first needle 421 can move in the X direction above the reagent reaction tube 21. The operation is simple and reliable.

[0059] Meanwhile, a second sliding guide groove 2214 is formed on the first mounting base 221. The second sliding guide groove 2214 is constructed to extend in the vertical direction. The first mounting base 221 includes a first sliding component 2215. The first sliding component 2215 is slidably mounted on the second sliding guide groove 2214 to achieve sliding engagement. The first needle 421 is relatively fixed to the first sliding component 2215. Thus, the position of the first sliding component 2215 on the first mounting base 221 can be changed by sliding, so that the first needle 421 can move in the Z direction above the reagent reaction tube 21.

[0060] Furthermore, the second needle 611 is movably mounted on the mounting bracket 22, which has a second mounting seat 222 for mounting the second needle 611. The second mounting seat 222 is configured to be adjustable relative to the mounting bracket 22, allowing the position of the second needle 611 to be adjusted by changing its position on the bracket 22. This enables selective movement of the second needle 611 above the reagent reaction tube 21. The second needle 611 is detachably mounted on the second mounting seat 222, facilitating connection and disconnection. This allows for easy installation and removal of the second needle 611, enabling its replacement with pipette tips, microsyringes, multi-channel dispensing heads, etc., to adapt to different pretreatment steps and their required volume ranges. Additionally, it facilitates replacement of the second needle 611 in case of damage or failure, reducing subsequent maintenance costs.

[0061] The second mounting base 222 is adjustable in position relative to the mounting bracket 22, such as... Figure 1 , Figure 2 , Figure 7 , Figure 8 and Figure 10 A first sliding guide groove 223 can be formed on the mounting bracket 22. The first sliding guide groove 223 is constructed to extend in the left and right direction, and the second mounting base 222 can form a second slider. The second slider is slidably installed in the first sliding guide groove 223 to achieve sliding engagement. In this way, the position of the second mounting base 222 on the mounting bracket 22 can be changed by sliding, so that the second needle 611 can move in the X direction above the reagent reaction tube 21. The operation is simple and reliable.

[0062] Meanwhile, a third sliding guide groove 2223 is formed on the second mounting base 222. The third sliding guide groove 2223 is constructed to extend in the vertical direction. The second mounting base 222 includes a second sliding component 2224. The second sliding component 2224 is slidably mounted on the third sliding guide groove 2223 to achieve sliding engagement. The second needle 611 is relatively fixed to the second sliding component 2224. Thus, the position of the second sliding component 2224 on the second mounting base 222 can be changed by sliding, so that the second needle 611 can move in the Z direction above the reagent reaction tube 21.

[0063] It should be noted that, as Figure 1 , Figure 7 and Figure 10As shown, two spaced-apart slide rails 23 are provided at the bottom of the second device body 2. Both slide rails 23 extend in the front-to-back direction and are parallel in the left-to-right direction. The mounting bracket 22 is slidably mounted on the slide rails 23, allowing the mounting bracket 22 to move relative to the second device body 2 in the front-to-back direction. This allows the first mounting base 221 and the second mounting base 222 to move relative to the second device body 2 in the front-to-back direction. Both the first mounting base 221 and the second mounting base 222 are also configured to move relative to the mounting bracket 22 in the left-to-right direction. The moving component 2215 is movable in the vertical direction relative to the first mounting base 221, and the second sliding component 2224 is movable in the vertical direction relative to the second mounting base 222. This allows the first needle 421 and the second needle 611 to selectively move in three degrees of freedom in the X, Y, and Z directions above the reagent reaction tube 21. This enables precise adjustment of the positions of the first needle 421 and the second needle 611, ensuring the reliability of selectively moving the first needle 421 and the second needle 611 above the corresponding reagent reaction tube 21.

[0064] In some embodiments, the first mounting base 221 is provided with a first snap-fit ​​portion 2211 and / or a first magnetic element 2212, and the first needle 421 is snap-fitted with the first snap-fit ​​portion 2211, and the first needle 421 is adapted to be magnetically fixed to the first magnetic element 2212; and / or, the second mounting base 222 is provided with a second snap-fit ​​portion 2221 and / or a second magnetic element 2222, and the second needle 611 is snap-fitted with the second snap-fit ​​portion 2221, and the second needle 611 is adapted to be magnetically fixed to the second magnetic element 2222.

[0065] Specifically, the first needle 421 is detachably mounted on the first mounting base 221. The first mounting base 221 is provided with a first snap-fit ​​part 2211, which is used to snap-fit ​​the first needle 421. For example, the first snap-fit ​​part 2211 can be constructed as a hook and a slot can be formed on the first needle 421, or the first snap-fit ​​part 2211 can be constructed as a slot and a hook can be formed on the first needle 421. The snap-fit ​​engagement between the first needle 421 and the first mounting base 221 can be achieved through the hook and the slot. The snap-fit ​​engagement method can realize the detachable connection between the first needle 421 and the first mounting base 221, thereby facilitating the installation or removal of the first needle 421.

[0066] In such Figure 1 , Figure 2 , Figure 7 , Figure 8 and Figure 10In the embodiment shown, the first latching part 2211 is constructed as two opposing latches, which are spaced apart by a distance and have a receiving space between them. The first needle 421 can be inserted into the receiving space. At the same time, two mutually opposing slots are formed on the first needle 421. The two latches and the two slots are respectively arranged in a one-to-one correspondence. Thus, when the first needle 421 is inserted into the receiving space, the two latches can engage with the two slots respectively, thereby achieving reliable fixation of the first needle 421.

[0067] Furthermore, a first magnetic element 2212 can be provided on the first mounting base 221. The first magnetic element 2212 is used to magnetically fix the first needle 421. A first mating element 4211 can be provided on the first needle 421. For example, the first magnetic element 2212 can be constructed as a magnet, and the first mating element 4211 can be made of magnetic metal. Alternatively, the first magnetic element 2212 can be made of magnetic metal, and the first mating element 4211 can be constructed as a magnet. Through the attraction of the magnet to the magnetic metal, the first mounting base 221 can magnetically fix the first needle 421. The magnetic fixation method can achieve the detachable connection between the first needle 421 and the first mounting base 221, thereby facilitating the installation or removal of the first needle 421.

[0068] In such Figures 9-10 In the embodiment shown, the first magnetic component 2212 is constructed as three magnets arranged sequentially along the extension direction of the first mounting base 221. Meanwhile, the first mating component 4211 consists of three corresponding magnetic metal sheets. When the first needle 421 is brought close to the first mounting base 221, the magnets can attract the magnetic metal sheets, thereby fixing the first needle 421. Furthermore, by increasing the number of magnets, the magnets' attraction to the first needle 421 can be increased, ensuring reliable fixing of the first needle 421.

[0069] And such as Figure 1 , Figure 2 , Figures 7-10 As shown, the first mounting base 221 is provided with a first snap-fit ​​part 2211 and a first magnetic element 2212, so that the first needle 421 can be installed on the first mounting base 221 by snap-fit ​​and magnetic attraction at the same time, which can ensure the reliable fixation of the first needle 421, and thus ensure the reliable operation of the first needle 421. The first sliding component 2215 includes the first snap-fit ​​part 2211 and the first magnetic element 2212.

[0070] Meanwhile, the second needle 611 is detachably mounted on the second mounting base 222. The second mounting base 222 is provided with a second snap-fit ​​part 2221, which is used to snap-fit ​​the second needle 611. For example, the second snap-fit ​​part 2221 can be constructed as a hook and a slot can be formed on the second needle 611, or the second snap-fit ​​part 2221 can be constructed as a slot and a hook can be formed on the second needle 611. The snap-fit ​​engagement between the second needle 611 and the second mounting base 222 can be achieved through the hook and the slot. The snap-fit ​​engagement method can realize the detachable connection between the second needle 611 and the second mounting base 222, thereby facilitating the installation or removal of the second needle 611.

[0071] In such Figure 1 , Figure 2 , Figure 7 , Figure 8 and Figure 10 In the embodiment shown, the second latching part 2221 is constructed as two opposing latches, which are spaced apart and have a receiving space between them. The second needle 611 can be inserted into the receiving space. At the same time, two opposing slots are formed on the second needle 611. The two latches and the two slots are respectively arranged in a one-to-one correspondence. Thus, when the second needle 611 is inserted into the receiving space, the two latches can engage with the two slots respectively, thereby achieving reliable fixation of the second needle 611.

[0072] Furthermore, a second magnetic element 2222 can be provided on the second mounting base 222. The second magnetic element 2222 is used to magnetically fix the second needle 611. A second mating part can be provided on the second needle 611. For example, the second magnetic element 2222 can be constructed as a magnet, and the second mating part can be made of magnetic metal. Alternatively, the second magnetic element 2222 can be made of magnetic metal, and the second mating part can be constructed as a magnet. The magnetic fixation of the second mounting base 222 to the second needle 611 can be achieved through the attraction of the magnet to the magnetic metal. The magnetic fixation method can achieve the detachable connection between the second needle 611 and the second mounting base 222, thereby facilitating the installation or removal of the second needle 611.

[0073] In such Figures 9-10 In the embodiment shown, the second magnetic component 2222 is constructed as three magnets arranged sequentially along the extension direction of the second mounting base 222. At the same time, the second mating component is three corresponding magnetic metal sheets. When the second needle 611 is brought close to the second mounting base 222, the magnets can attract the magnetic metal sheets, thereby fixing the second needle 611. Furthermore, by increasing the number of magnets, the attraction capacity of the magnets for the second needle 611 can be increased, ensuring reliable fixing of the second needle 611.

[0074] And such as Figure 1 , Figure 2 , Figures 7-10 As shown, the second mounting base 222 is provided with a second snap-fit ​​part 2221 and a second magnetic element 2222, so that the second needle 611 can be installed on the second mounting base 222 by snap-fit ​​and magnetic attraction at the same time, which can ensure reliable fixation of the second needle 611, thereby ensuring the reliable operation of the second needle 611. The second sliding component 2224 includes the second snap-fit ​​part 2221 and the second magnetic element 2222.

[0075] Furthermore, it should be noted that in practice, both the first needle 421 and the second needle 611 can be constructed as stainless steel needles, and the first needle 421 and the second needle 611 can be rigidly connected to the outlet of the reagent extraction tube 4 and the inlet of the waste liquid collection tube 6 respectively through Luer connectors, etc., so that the outlet of the reagent extraction tube 4 and the inlet of the waste liquid collection tube 6 can be compatible with standard syringe interfaces to ensure sealing and pressure resistance.

[0076] In some embodiments, the first device body 1 and the second device body 2 are spaced apart, and both the reagent extraction tube 4 and the waste liquid collection tube 6 are at least partially constructed as flexible tubes.

[0077] Specifically, the first device body 1 is used to place the reagent bottle 11 and the waste liquid collection bottle 12, and the second device body 2 is used to place the reagent reaction tube 21. The first device body 1 and the second device body 2 are distributed separately, so that the first device body 1 and the second device body 2 are separated by a distance. This makes it easier to place the reagent bottle 11 and the waste liquid collection bottle 12 in the first device body 1 and the reagent reaction tube 21 in the second device body 2, avoiding confusion. Furthermore, both the reagent extraction tube 4 and the waste liquid collection tube 6 are at least partially constructed as flexible tubes. The flexible tubes can produce a certain elastic deformation under stress and are not easy to break. They can adapt to a certain degree of bending. The flexible tubes facilitate reagent filling and waste liquid recycling and help extend the service life of the reagent extraction tube 4 and the waste liquid collection tube 6.

[0078] It should be noted that when using the protein strip sample pretreatment device 100 to pretreatment protein strip samples, if decolorization can be performed first, the cut, untreated protein strip sample can be placed into the reagent reaction tube 21. Then, the reagent extraction pump 3 is started through the pump control module, and the reagent extraction tube 4 extracts a quantitative amount of decolorizing reagent from the reagent bottle 11 and injects it into the corresponding reagent reaction tube 21 through the first needle 421. After the protein strip sample reacts with the decolorizing reagent, i.e., after the decolorization is completed, the pump... The pump control module activates the waste liquid collection pump 5, extracting the waste liquid from the reagent reaction tube 21 through the second needle 611 and transferring it to the waste liquid collection bottle 12 through the waste liquid collection tube 6. Then, decolorization and drying can be performed. The pump control module activates the reagent extraction pump 3, extracting sufficient acetonitrile from the reagent bottle 11 and injecting it into the reagent reaction tube 21 through the first needle 421. After decolorization and drying are completed, the pump control module activates the waste liquid collection pump 5, transferring the waste liquid from the reagent reaction tube 21 to the waste liquid collection bottle 12 through the second needle 611. All other pretreatment steps follow the same operating procedure.

[0079] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0080] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A protein gel strip sample pretreatment device, characterized in that, include: The first device body (1) and the second device body (2) are provided with a reagent bottle (11) and a waste liquid collection bottle (12) in the first device body (1) and a reagent reaction tube (21) in the second device body (2). The reagent extraction pump (3), reagent extraction tube (4), waste liquid collection pump (5) and waste liquid collection tube (6) are provided. The reagent extraction tube (4) is connected to a first needle (421). The reagent extraction pump (3) is connected to the reagent extraction tube (4) and is used to drive the reagent extraction tube (4) to draw the reagent in the reagent bottle (11) to the first needle (421) and from the first needle (421) into the reagent reaction tube (21). The waste liquid collection tube (6) is connected to a second needle (611). The waste liquid collection pump (5) is connected to the waste liquid collection tube (6) and is used to drive the waste liquid collection tube (6) to draw the waste liquid in the reagent reaction tube (21) to the waste liquid collection bottle (12) through the second needle (611).

2. The protein glue strip sample pretreatment device according to claim 1, characterized in that, The reagent extraction pump (3) and the waste liquid collection pump (5) are both installed on the first equipment body (1), and the reagent extraction pump (3) and the waste liquid collection pump (5) are spaced apart and distributed on the first equipment body (1).

3. The protein gel strip sample pretreatment device according to claim 2, characterized in that, The first device body (1) includes a first mounting surface (13), a second mounting surface (14) and a connecting side surface (15). The first mounting surface (13) and the second mounting surface (14) are located on opposite sides of the first device body (1). The reagent extraction pump (3) is installed on the first mounting surface (13), and the waste liquid collection pump (5) is installed on the second mounting surface (14). The connecting side (15) is connected between the first mounting surface (13) and the second mounting surface (14). The connecting side (15) is provided with a receiving groove, and the reagent bottle (11) and the waste liquid collection bottle (12) are both placed in the receiving groove.

4. The protein gel strip sample pretreatment device according to claim 1, characterized in that, The reagent extraction pump (3) is connected to a reagent extraction valve (31), and the reagent extraction valve (31) is provided with at least one first inlet (311) and at least one first outlet. The reagent extraction tube (4) includes a first inlet tube (41) and a first outlet tube (42). One end of the first inlet tube (41) is connected to the first inlet port (311) and the other end is adapted to extend into the reagent bottle (11). One end of the first outlet tube (42) is connected to the first outlet port and the other end is provided with the first needle (421).

5. The protein gel strip sample pretreatment device according to claim 1, characterized in that, The waste liquid collection pump (5) is connected to a waste liquid collection valve (51), and the waste liquid collection valve (51) is provided with at least one second inlet and at least one second outlet (512). The waste liquid collection tube (6) includes a second inlet tube (61) and a second outlet tube (62). One end of the second inlet tube (61) is connected to the second needle (611) and the other end is connected to the second inlet. One end of the second outlet tube (62) is connected to the second outlet (512) and the other end is adapted to extend into the waste liquid collection bottle (12).

6. The protein gel strip sample pretreatment device according to claim 1, characterized in that, It also includes a pump control module, wherein the reagent extraction pump (3) and the waste liquid collection pump (5) are both electrically connected to the pump control module, and the pump control module is used to independently control the reagent extraction pump (3) and the waste liquid collection pump (5) to operate selectively.

7. The protein glue strip sample preparation device according to any one of claims 1-6, wherein, The second device body (2) is equipped with a mounting frame (22). Both the first needle (421) and the second needle (611) can be movably mounted on the mounting frame (22) and can selectively move in three degrees of freedom in the X, Y and Z directions above the reagent reaction tube (21).

8. The protein gel strip sample pretreatment device according to claim 7, characterized in that, The mounting bracket (22) is provided with a first mounting seat (221), the position of the first mounting seat (221) relative to the mounting bracket (22) is adjustable, and the first needle (421) is detachably mounted on the first mounting seat (221). And / or, the mounting bracket (22) is provided with a second mounting seat (222), the second mounting seat (222) is positionally adjustable relative to the mounting bracket (22), and the second needle (611) is detachably mounted on the second mounting seat (222).

9. The protein gel strip sample pretreatment device according to claim 8, characterized in that, The first mounting base (221) is provided with a first snap-fit ​​part (2211) and / or a first magnetic element (2212). The first needle (421) is snap-fitted with the first snap-fit ​​part (2211) and the first needle (421) is adapted to be magnetically fixed to the first magnetic element (2212). And / or, the second mounting base (222) is provided with a second snap-fit ​​portion (2221) and / or a second magnetic element (2222), the second needle (611) is snap-fitted with the second snap-fit ​​portion (2221), and the second needle (611) is adapted to be magnetically fixed to the second magnetic element (2222).

10. The protein glue strip sample preparation device according to any one of claims 1-6, wherein, The first device body (1) and the second device body (2) are spaced apart, and the reagent extraction tube (4) and the waste liquid collection tube (6) are at least partially constructed as flexible tubes.

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