Magnetic bead method biological sample extraction apparatus

Abstract

The disclosure provides a magnetic bead method biological sample extraction device, and belongs to the technical field of biological equipment. The magnetic bead method biological sample extraction device comprises a base, a reagent box support, an extraction mechanism and a concentration mechanism; the reagent box support is connected with the base, and the reagent box support is used for accommodating a reagent box; the extraction mechanism is in transmission connection with the base, the extraction mechanism is used for extracting a biological sample, and the biological sample is mixed with a solvent in the reagent box; the concentration mechanism is in transmission connection with the extraction mechanism, and the concentration mechanism is used for conveying inert gas into the reagent box to evaporate the solvent. By using the disclosure, the pollution risk of the biological sample can be reduced.

Description

Technical Field

[0001] This disclosure belongs to the field of biological equipment technology, and specifically relates to a magnetic bead-based biological sample extraction device. Background Technology

[0002] With the development of modern biological technology, biological detection technologies based on biological samples such as nucleic acids are playing an increasingly important role in many fields.

[0003] Currently, biological samples typically undergo two steps before testing: extraction and concentration. Extraction is usually performed using extraction equipment, while concentration is usually performed using nitrogen blowing equipment.

[0004] However, in the above process, after the extraction of biological samples is completed using the extraction equipment, the relevant biological samples need to be manually transferred to the nitrogen blowing concentration equipment, which makes the relevant biological samples have a high risk of contamination. Utility Model Content

[0005] This disclosure provides a magnetic bead-based biological sample extraction device, which includes a base, a reagent kit holder, an extraction mechanism, and a concentration mechanism.

[0006] The reagent kit holder is connected to the base, and the reagent kit holder is used to hold the reagent kit;

[0007] The extraction mechanism is connected to the base via a transmission. The extraction mechanism is used to extract biological samples and mix the biological samples with the solvent in the kit.

[0008] The concentration mechanism is connected to the extraction mechanism by a drive, and the concentration mechanism is used to deliver gas into the reagent kit to evaporate the solvent.

[0009] In one possible implementation, the extraction mechanism includes a first drive mechanism, a frame, and an extraction mechanism body;

[0010] The first drive mechanism is connected to the base and is also connected to the frame in a transmission manner. The first drive mechanism is used to drive the frame to move in the horizontal direction.

[0011] The extraction mechanism body is connected to the frame.

[0012] In one possible implementation, the first drive mechanism includes a first drive motor, a first lead screw, and a first transmission component;

[0013] The first lead screw extends horizontally and is connected to the output shaft of the first drive motor;

[0014] The first transmission component is circumferentially limited by the base, and the first transmission component is sleeved outside the first lead screw and connected to the frame.

[0015] In one possible implementation, the extraction mechanism body includes a second driving mechanism, a magnetic rod sleeve assembly, a third driving mechanism, and a magnetic rod assembly;

[0016] The second drive mechanism is connected to the frame and is connected to the magnetic rod sleeve assembly in a transmission manner. The second drive mechanism is used to drive the magnetic rod sleeve assembly to move in the vertical direction.

[0017] The third driving mechanism is connected to the second driving mechanism and is connected to the magnetic rod assembly in a transmission manner. The third driving mechanism is used to drive the magnetic rod assembly to move in the vertical direction.

[0018] In one possible implementation, the second drive mechanism includes a second drive motor, a second lead screw, and a second transmission component. The second lead screw extends vertically and is connected to the output shaft of the second drive motor. The second transmission component is circumferentially limited by the frame. The second transmission component is sleeved outside the second lead screw and connected to the magnetic rod sleeve assembly.

[0019] The third drive mechanism includes a connector, a third drive motor, a third lead screw, and a third transmission component. The connector is located on the side of the second transmission component away from the frame and is connected to the second transmission component. The third drive motor is connected to the connector. The third lead screw extends vertically and is connected to the output shaft of the third drive motor. The third transmission component is circumferentially limited by the connector. The third transmission component is sleeved on the outside of the third lead screw and is connected to the magnetic rod assembly.

[0020] In one possible implementation, the concentration mechanism includes a fourth drive mechanism and an air blowing assembly;

[0021] The fourth drive mechanism is connected to the frame and is driven by the air blowing assembly. The fourth drive mechanism is used to drive the air blowing assembly to move in the vertical direction.

[0022] In one possible implementation, the fourth drive mechanism includes a fourth drive motor, a fourth lead screw, and a fourth transmission component;

[0023] The fourth lead screw extends vertically and is connected to the output shaft of the fourth drive motor;

[0024] The fourth transmission component is circumferentially limited by the frame, and the fourth transmission component is sleeved and installed outside the fourth lead screw;

[0025] The blowing assembly includes a blowing bracket and an air needle. The blowing bracket is connected to the fourth transmission component, and the air inlet of the blowing bracket is connected to an external air source through an air pipe.

[0026] One end of the air needle is connected to the air outlet of the air blowing bracket, and the other end of the air needle is used to extend into the reagent kit.

[0027] In one possible implementation, the concentration mechanism further includes a plurality of ventilation fans for ventilating the interior of the magnetic bead-based biological sample extraction device.

[0028] In one possible implementation, the magnetic bead-based biological sample extraction device further includes a temperature control component, which includes a heating element, a temperature control fan, and a control unit.

[0029] The heating element is arranged opposite to the reagent kit holder;

[0030] The temperature-controlled fan is connected to the base and is arranged opposite to the heating element;

[0031] The control unit is electrically connected to the heating element and the temperature-controlled fan, respectively.

[0032] In one possible implementation, the magnetic bead-based biological sample extraction device includes at least one cable chain for housing a cable for a drive motor.

[0033] The technical solution provided in this disclosure includes at least the following beneficial effects:

[0034] This disclosure provides a magnetic bead-based biological sample extraction device that integrates an extraction mechanism and a concentration mechanism. The magnetic bead-based biological sample extraction device can complete the extraction and concentration steps separately. Compared with related technologies, the concentration step can be completed without manual transfer after the extraction step, reducing the risk of contamination.

[0035] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit this disclosure. Attached Figure Description

[0036] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this disclosure and, together with the description, serve to explain the principles of this disclosure. In the drawings:

[0037] Figure 1 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0038] Figure 2This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0039] Figure 3 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0040] Figure 4 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0041] Figure 5 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0042] Figure 6 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure;

[0043] Figure 7 This is a schematic diagram of the structure of a magnetic bead-based biological sample extraction device provided in an embodiment of this disclosure.

[0044] Legend

[0045] 100. Reagent kit;

[0046] 1. Base;

[0047] 11. Base plate; 12. Pallet;

[0048] 111. Guide rail structure; 112. Receiving groove; 113. Fan mounting hole; 121. Handle;

[0049] 2. Reagent kit holder;

[0050] 3. Extraction facility;

[0051] 31. First drive mechanism; 32. Frame; 33. Extraction mechanism body;

[0052] 311. First drive motor; 312. First lead screw; 313. First transmission component; 314. First lead screw support; 315. First slide rail assembly; 321. First plate; 322. Second plate; 323. Third plate;

[0053] 3151, First slide rail; 3152, First slider;

[0054] 331. Second drive mechanism; 332. Magnetic rod sleeve assembly; 333. Third drive mechanism; 334. Magnetic rod assembly;

[0055] 33151, Second slide rail; 33152, Second slider;

[0056] 3311. Second drive motor; 3312. Second lead screw; 3313. Second transmission component; 3314. Second lead screw support; 3315. Second slide rail assembly;

[0057] 3331. Connecting component; 3332. Third drive motor; 3333. Third lead screw; 3334. Third transmission component; 3335. Third slide rail assembly;

[0058] 33351, Third slide rail; 33352, Third slider;

[0059] 4. Concentration mechanism;

[0060] 41. Fourth drive mechanism; 42. Air blowing assembly; 43. Ventilation fan;

[0061] 411. Fourth drive motor; 412. Fourth lead screw; 413. Fourth transmission component; 414. Fourth lead screw support; 415. Fourth slider;

[0062] 421. Inflation support; 422. Air needle;

[0063] 5. Temperature control components;

[0064] 51. Heating element; 52. Temperature-controlled fan;

[0065] 511. Heating block;

[0066] 6. Cable chain;

[0067] 7. Cover;

[0068] 71. Ventilated areas;

[0069] 8. Mounting bracket.

[0070] The accompanying drawings have illustrated specific embodiments of this disclosure, which will be described in more detail below. These drawings and descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concepts of this disclosure to those skilled in the art through reference to particular embodiments. Detailed Implementation

[0071] To make the objectives, technical solutions, and advantages of this disclosure clearer, the embodiments of this disclosure will be further described in detail below with reference to the accompanying drawings.

[0072] With the development of modern biological technology, bioassay technologies based on biological samples such as nucleic acids are playing an increasingly important role in many fields. Currently, the detection of biological samples typically involves two steps: extraction and concentration. Extraction is usually performed using extraction equipment, while concentration is typically performed using nitrogen blowing equipment. However, after the extraction step, the biological sample needs to be manually transferred to the nitrogen blowing concentration equipment, which increases the risk of contamination.

[0073] To address the aforementioned problems, this disclosure provides a magnetic bead-based biological sample extraction device. Figure 1 This is a schematic diagram of a magnetic bead-based biological sample extraction device. (See attached diagram) Figure 1 The magnetic bead-based biological sample extraction device has a housing 7, which houses functional components. Technicians can open the door structure on the housing 7 and place the reagent kit 100 on the reagent kit holder 2 to complete the subsequent extraction and concentration of biological samples. The specific process will be described below.

[0074] Figure 2 See the schematic diagram of the functional components of the magnetic bead-based biological sample extraction device. Figure 2 The magnetic bead-based biological sample extraction device includes a base 1, a reagent kit holder 2, an extraction mechanism 3, and a concentration mechanism 4.

[0075] The reagent kit holder 2 is connected to the base 1 and is used to hold the reagent kit 100. The extraction mechanism 3 is drivenly connected to the base 1 and is used to extract biological samples. The concentration mechanism 4 is drivenly connected to the extraction mechanism 3 and is used to deliver gas into the reagent kit 100 to evaporate the solvent. The gas delivered into the reagent kit 100 by the concentration mechanism 4 can be an inert gas or air, and this disclosure does not limit it.

[0076] Using the technical solution provided in this embodiment, the magnetic bead biological sample extraction device integrates an extraction mechanism 3 and a concentration mechanism 4. The magnetic bead biological sample extraction device can complete the extraction step and the concentration step respectively. Compared with related technologies, the concentration step can be completed without manual transfer after the extraction step, which reduces the risk of contamination.

[0077] The following is a detailed description of the structure of the magnetic bead-based biological sample extraction device:

[0078] I. Base 1 and Reagent Kit Holder 2

[0079] The base 1 is a component used to install other components in the magnetic bead-based biological sample extraction device, and the reagent kit holder 2 is a component used to install the reagent kit 100 in the magnetic bead-based biological sample extraction device.

[0080] In some examples, the base 1 includes a base plate 11 and a tray 12, with the tray 12 slidably connected to the base plate 11.

[0081] like Figure 3 As shown, the upper wall of the base plate 11 is provided with two guide rail structures 111. These two guide rail structures 111 are spaced apart and parallel to each other. See [reference needed] Figure 3 And refer to Figure 2 The tray 12 is slidably connected to the two guide rail structures 111 respectively. See also Figure 2 The tray 12 has a handle 121 at one end, and the reagent kit holder 2 is disposed on the upper wall of the tray 12. In implementation, refer to... Figure 1 Technicians can pull the handle 121 to slide the tray 12 relative to the base plate 11 to the outside of the cover 7. Then, the technicians can place the reagent kit 100 in the reagent kit holder 2, and push the handle 121 to slide the tray 12 relative to the base plate 11 to the inside of the cover 7. Finally, the technicians can operate the internal functional components of the magnetic bead biological sample extraction device through the program to complete the extraction and concentration of biological samples.

[0082] like Figure 3 As shown, the base plate 11 has a rectangular plate-like structure and a receiving groove 112 extending through the upper and lower walls. The receiving groove 112 is a strip-shaped groove and extends horizontally. In some examples, the extending direction of the receiving groove 112 is parallel to the extending direction of the guide rail structure 111.

[0083] like Figure 2 As shown, there can be multiple reagent kit holders 2. For example, there can be two reagent kit holders 2, which are distributed at intervals along the extension direction of the above-mentioned receiving groove 112.

[0084] Specifically, see Figure 2 Each reagent kit holder 2 may have multiple reagent kit receiving slots, which are strip-shaped slots extending in the same direction as the receiving slots 112 on the base plate 11. Each reagent kit receiving slot is used to receive one reagent kit. Exemplarily, the multiple reagent kit receiving slots are spaced apart along the extension direction perpendicular to the receiving slots 112.

[0085] In some possible embodiments, the magnetic bead-based biological sample extraction device also includes a temperature control component 5, which includes a heating element 51 and a temperature control fan 52.

[0086] like Figure 3 As shown, the base 1 has a fan mounting hole 113 that extends through the upper and lower walls.

[0087] Specifically, see Figure 2 and Figure 3The magnetic bead-based biological sample extraction device includes two reagent kit holders 2. Correspondingly, the base 1 has two fan mounting holes 113, each fan mounting hole 113 being arranged opposite to one reagent kit holder 2. Each fan mounting hole 113 is used to install a temperature-controlled fan 52, so that each temperature-controlled fan 52 is arranged opposite to one reagent kit holder 2. Further, a heating element 51 is provided between each reagent kit holder 2 and the base 1. That is, the magnetic bead-based biological sample extraction device includes two reagent kit holders 2, two heating elements 51, and two temperature-controlled fans 52, with each reagent kit holder 2 and each temperature-controlled fan 52 distributed on both sides of a heating element 51 in the vertical direction.

[0088] In some examples, each heating element 51 includes a plurality of heating blocks 511, which are spaced apart in a horizontal direction. For example, each heating element 51 may include two heating blocks 511.

[0089] In implementation, the heating element 51 and the temperature-controlled fan 52 are electrically connected to a control unit (not shown in the figure), which controls the operating status of the heating element 51 and the temperature-controlled fan 52 respectively. When the reagent kit 100 needs to be heated, the control unit can control the heating element 51 to be in the working state and control the temperature-controlled fan 52 to be in the non-working state, so that the heating element 51 heats the internal solution of the reagent kit 100, thereby raising the temperature of the internal solution of the reagent kit 100. Correspondingly, when the reagent kit 100 needs to be cooled (or does not need to be heated), the control unit can control the heating element 51 to be in the non-working state and control the temperature-controlled fan 52 to be in the working state, so that the heating element 51 stops heating and the heat from the surrounding environment of the heating element 51 is discharged through the temperature-controlled fan 52 and the ventilation fan 43, thereby lowering the temperature of the internal solution of the reagent kit 100 (or preventing it from rising further).

[0090] II. Extraction Agency 3

[0091] Extraction mechanism 3 is a component in the magnetic bead-based biological sample extraction device used to perform the extraction step.

[0092] like Figure 2 As shown, the extraction mechanism 3 includes a first drive mechanism 31, a frame 32, and an extraction mechanism body 33.

[0093] The first drive mechanism 31 is connected to the base 1 and is also connected to the frame 32 in a transmission manner. The first drive mechanism 31 is used to drive the frame 32 to move in the extension direction of the receiving groove of the base 1. The extraction mechanism body 33 is connected to the frame 32.

[0094] In implementation, see Figure 2The reagent kit 100 has multiple independent cavities, which are spaced apart along the extension direction of the receiving groove of the base 1. The first drive mechanism 31 controls the frame 32 to move along the extension direction of the receiving groove, thereby driving the extraction mechanism body 33 to move along the extension direction of the receiving groove, so that the extraction mechanism body 33 can contact each of the multiple cavities to complete the extraction step. The specific process of the extraction step is not the focus of this disclosure; readers can find relevant information for further details, and it will not be described in detail here.

[0095] First drive mechanism 31

[0096] Specifically, see Figure 3 The first drive mechanism 31 includes a first drive motor 311, a first lead screw 312, and a first transmission member 313. The first drive motor 311 is located at the first end of the receiving groove and is connected to the base 1. The output shaft of the first drive motor 311 points to the second end of the receiving groove. The first lead screw 312 is located inside the receiving groove and extends in the same direction as the receiving groove. The first end of the first lead screw 312 is fixedly connected to the output shaft of the first drive motor 311, and the second end of the first lead screw 312 is rotatably connected to the base 1. The first transmission member 313 is located inside the receiving groove and is fitted outside the first lead screw 312. The first transmission member 313 is circumferentially limited by the base 1, and the first transmission member 313 has an internal thread that is compatible with the first lead screw 312.

[0097] In implementation, the rotation of the output shaft of the first drive motor 311 drives the first lead screw 312 to rotate, thereby creating a tendency to drive the first transmission component 313 to rotate circumferentially. Since the first transmission component 313 is circumferentially limited by the base 1, this rotational tendency is converted into a tendency to move along the axial direction of the first lead screw 312. It is easy to understand that when the output shaft of the first drive motor 311 switches between forward and reverse rotation, the corresponding direction of movement of the first transmission component 313 switches in the opposite direction.

[0098] In some examples, the first drive mechanism 31 also includes a first lead screw support 314. For example... Figure 3 As shown, the first lead screw support 314 is located at the second end of the receiving groove, and the first lead screw support 314 is fixedly connected to the base 1. The second end of the first lead screw 312 extends into the interior of the first lead screw support 314 and is rotatably connected to the first lead screw support 314. In this way, the machining difficulty of the base 1 can be reduced.

[0099] In some examples, the first drive mechanism 31 also includes a first slide rail assembly 315. For example... Figure 3 As shown, the first slide rail assembly 315 includes a first slide rail 3151 and a first slider 3152. (See also...) Figure 3The first slide rail 3151 is fixed to the upper wall of the base 1 and extends in the same direction as the receiving groove. The first slider 3152 is slidably connected to the first slide rail 3151 and is fixedly connected to the frame 32.

[0100] For example, the first slide rail assembly includes two first slide rails 3151 and two first sliders 3152. The two first slide rails 3151 are arranged in parallel and distributed on both sides in the width direction of the receiving groove. Each first slider 3152 is slidably connected to one of the first slide rails 3151.

[0101] In this way, by setting the first slide rail assembly 315, the horizontal movement stability of the frame 32 and the extraction mechanism body 33 can be improved.

[0102] Frame 32

[0103] In some examples, the frame 32 includes a first plate 321, a second plate 322, and a third plate 323.

[0104] like Figure 3 As shown, the first plate 321 is arranged horizontally, and its lower wall is connected to the first slider 3152 and the first transmission member 313. The second plate 322 and the third plate 323 are arranged vertically, perpendicular to each other and connected to the first plate 321.

[0105] For example, the third plate 323 is connected to the second plate 322 at one side edge, so that a large clearance area can be formed between the third plate 323 and the second plate 322 for mounting the second drive mechanism.

[0106] Optionally, the frame 32 also includes a reinforcing plate, which is connected to the other edge of the second plate 322, thereby improving the overall strength of the frame 32.

[0107] Extraction mechanism body 33

[0108] like Figure 4 As shown, the extraction mechanism body 33 includes a second driving mechanism 331, a magnetic rod sleeve assembly 332, a third driving mechanism 333, and a magnetic rod assembly 334.

[0109] The second drive mechanism 331 is connected to the frame 32 and is drive-connected to the magnetic rod sleeve assembly 332. The second drive mechanism 331 is used to drive the magnetic rod sleeve assembly 332 to move vertically. The third drive mechanism 333 is connected to the second drive mechanism 331 and is drive-connected to the magnetic rod assembly 334. The third drive mechanism 333 is used to drive the magnetic rod assembly 334 to move vertically. The magnetic rod sleeve assembly 332 includes a connected magnetic rod sleeve support and a magnetic rod sleeve. The magnetic rod assembly 334 includes a connected magnetic rod support and a magnetic rod. The magnetic rod sleeve and the magnetic rod are arranged coaxially.

[0110] In practice, the magnetic rod holder is positioned above the magnetic rod sleeve holder. The second driving mechanism 331 drives the magnetic rod sleeve holder and the magnetic rod holder together to move vertically. During this process, the magnetic rod sleeve holder moves into or out of the reagent kit 100 under the drive of the second driving mechanism 331. The third driving mechanism 333 drives the magnetic rod holder to move vertically, causing the magnetic rod holder to move closer to or further away from the magnetic rod sleeve holder, thereby causing the magnetic rod to move into or out of the magnetic rod sleeve. When the magnetic rod sleeve is outside the reagent kit 100 or the magnetic rod is moved out of the magnetic rod sleeve, the magnetic rod cannot attract the magnetic beads in the reagent kit 100. When the magnetic rod sleeve is inside the reagent kit 100 and the magnetic rod moves into the magnetic rod sleeve, the magnetic rod can attract the magnetic beads in the reagent kit 100, causing the magnetic beads to accumulate on the surface of the magnetic rod sleeve for the extraction of biological samples.

[0111] By adopting the technical solution provided in this disclosure, when it is necessary to move the magnetic rod sleeve assembly 332 and the magnetic rod assembly 334 together, it is only necessary to control the second drive mechanism 331 to work. The third drive mechanism 333 only works when it is necessary to move the magnetic rod assembly 334 alone. Compared with the related technology, which requires controlling two sets of drive mechanisms separately to achieve the simultaneous movement of the magnetic rod sleeve assembly 332 and the magnetic rod assembly 334, the frequency of use of the third drive mechanism 333 can be reduced, thereby improving its service life.

[0112] Specifically, see [link to relevant documentation] Figure 4 The second drive mechanism 331 includes a second drive motor 3311, a second lead screw 3312, and a second transmission component 3313. (Reference) Figure 3The second drive motor 3311 is connected to the second plate 322. The output shaft of the second drive motor 3311 points vertically downward. The second lead screw 3312 extends vertically, with its first end connected to the output shaft of the second drive motor 3311 and its second end rotatably connected to the first plate 321. The second transmission component 3313 is fitted around the second lead screw 3312 and is connected to the magnetic rod sleeve bracket in the magnetic rod sleeve assembly 332. The second transmission component 3313 is circumferentially limited by the second plate 322 in the frame 32, and has an internal thread that mates with the second lead screw 3312.

[0113] In implementation, the rotation of the output shaft of the second drive motor 3311 drives the second lead screw 3312 to rotate, thereby creating a tendency to drive the second transmission component 3313 to rotate circumferentially. Since the second transmission component 3313 is circumferentially limited by the second plate 322, this rotational tendency is converted into a tendency to move along the axial direction of the second lead screw 3312. It is easy to understand that when the output shaft of the second drive motor 3311 switches between forward and reverse rotation, the corresponding direction of movement of the second transmission component 3313 switches in the opposite direction, that is, the second transmission component 3313 moves vertically upward or vertically downward, thereby driving the magnetic rod sleeve bracket and other components mounted on the magnetic rod sleeve bracket to move vertically upward or vertically downward together.

[0114] See in some examples Figure 4 The second drive mechanism 331 also includes a second lead screw support 3314, which is fixed to the upper wall of the second plate 322. The second end of the second lead screw 3312 is rotatably connected to the second lead screw support 3314. This reduces the machining difficulty of the second plate 322.

[0115] In some examples, the second drive mechanism 331 also includes a second slide rail assembly 3315. For example... Figure 7 As shown, the second slide rail assembly 3315 includes a second slide rail 33151 and a second slider 33152. See also... Figure 7 And refer to Figure 3 The second slide rail 33151 is fixed on the frame 32 and extends vertically. The second slider 33152 is slidably connected to the second slide rail 33151 and connected to the magnetic rod sleeve bracket in the magnetic rod sleeve assembly 332.

[0116] This improves the vertical stability of the magnetic rod sleeve assembly 332.

[0117] For example, the second slide rail 33151 can be arranged on the third plate 323, and the second slide rail 33151 is located on the side of the third plate 323 near the second lead screw 3312.

[0118] Further, see also Figure 4 The third drive mechanism 333 includes a connector 3331, a third drive motor 3332, a third lead screw 3333, and a third transmission component 3334. (See also...) Figure 4 And refer to Figure 3 The connecting member 3331 is located on the side of the second transmission member 3313 away from the second plate 322 and is connected to the second transmission member 3313. The connecting member 3331 includes a connecting plate and a support base. The connecting plate can be set perpendicular to the base 1 and is connected to the second transmission member 3313. The support base is located at the lower edge of the connecting plate and on the side of the connecting plate away from the second transmission member 3313, and is connected to the connecting plate. The third drive motor 3332 is located at the upper edge of the connecting plate and is connected to the connecting plate. The output shaft of the third drive motor 3332 points vertically downward. The third lead screw 3333 extends vertically. The first end of the third lead screw 3333 is connected to the output shaft of the third drive motor 3332, and the second end of the third lead screw 3333 is rotatably connected to the support base. The third transmission component 3334 is circumferentially limited by the connecting plate of the connecting component 3331, and the third transmission component 3334 has an internal thread that mates with the third lead screw 3333. The third transmission component 3334 is fitted outside the third lead screw 3333 and is connected to the magnetic rod support in the magnetic rod assembly 334.

[0119] In implementation, the rotation of the output shaft of the third drive motor 3332 drives the third lead screw 3333 to rotate, thereby creating a tendency to drive the third transmission component 3334 to rotate circumferentially. Since the third transmission component 3334 is circumferentially limited by the connecting member 3331, this rotational tendency is converted into a tendency to move along the axial direction of the third lead screw 3333. It is easy to understand that when the output shaft of the third drive motor 3332 switches between forward and reverse rotation, the corresponding direction of movement of the third transmission component 3334 switches in the opposite direction, that is, the third transmission component 3334 moves in a vertically upward or vertically downward direction, thereby driving the magnetic rod support to move vertically upward or vertically downward.

[0120] In some examples, the third drive mechanism 333 also includes a third slide rail assembly 3335. For example... Figure 7 As shown, the third slide rail assembly 3335 includes a third slide rail 33351 and a third slider 33352. See also... Figure 7 And refer to Figure 3The third slide rail 33351 is fixed on the frame 32 and extends vertically. The third slider 33352 is slidably connected to the third slide rail 33351 and connected to the magnetic rod support in the magnetic rod assembly 334.

[0121] This improves the vertical stability of the magnetic rod assembly 334.

[0122] For example, the third slide rail 33351 can be arranged on the third plate 323, and the third slide rail 33351 is located on the side of the third plate 323 away from the second lead screw 3312.

[0123] This prevents interference between the third slider 33352 and the second slider 33152.

[0124] By employing the technical solution provided in this embodiment, for the third drive mechanism 333, by providing a connecting member 3331 to the second transmission member 3313, the entire movement distance of the third transmission member 3334 on the third lead screw 3333 can be converted into the relative movement distance between the magnetic rod assembly 334 and the magnetic rod sleeve assembly 332. Therefore, the required transmission distance of the third lead screw 3333 is relatively short. Based on this, the pitch on the outer wall of the third lead screw 3333 can be set smaller. Correspondingly, the unit movement distance of the third transmission member 3334 driven by the third drive motor 3332 is relatively shorter. This improves the accuracy of the third drive motor 3332 driving the third transmission member 3334, thereby enabling more precise control of the movement distance of the magnetic rod relative to the magnetic rod sleeve, ultimately improving the extraction efficiency of biological samples.

[0125] III. Concentration Mechanism 4

[0126] Concentration unit 4 is a component in the magnetic bead-based biological sample extraction device used to perform the concentration step.

[0127] In some possible embodiments, such as Figure 5 As shown, the concentration mechanism 4 includes a fourth drive mechanism 41 and an air blowing assembly 42.

[0128] The fourth drive mechanism 41 is connected to the frame 32 and is connected to the air blowing assembly 42 in a transmission manner. The fourth drive mechanism 41 is used to drive the air blowing assembly 42 to move in the vertical direction.

[0129] During implementation, when a concentration step is required, the fourth drive mechanism 41 drives the blowing assembly 42 to move vertically downwards, extending the blowing assembly 42 into the interior of the reagent kit 100. This increases the gas flow rate inside the reagent kit 100, thereby increasing the solvent evaporation rate. Conversely, when a concentration step is not required, the fourth drive mechanism 41 drives the blowing assembly 42 to move vertically upwards, moving the blowing assembly 42 out of the reagent kit 100.

[0130] See in some examples Figure 5 The fourth drive mechanism 41 includes a fourth drive motor 411, a fourth lead screw 412, and a fourth transmission component 413.

[0131] Specifically, see Figure 5 And refer to Figure 4 The fourth drive motor 411 is located on the side of the third plate 323 away from the second drive mechanism 331, and is connected to the third plate 323. The output shaft of the fourth drive motor 411 points vertically downward. The fourth lead screw 412 extends vertically, and its first end is connected to the output shaft of the fourth drive motor 411. The fourth transmission member 413 is circumferentially limited by the frame 32 and the third plate 323. The fourth transmission member 413 is fitted outside the fourth lead screw 412, and has an internal thread that mates with the fourth lead screw 412. Further, see... Figure 5 The fourth drive mechanism 41 also includes a fourth lead screw support 414, which is located below the fourth drive motor 411 and connected to the third plate 323. The fourth lead screw support 414 is rotatably connected to the second end of the fourth lead screw 412.

[0132] In implementation, the rotation of the output shaft of the fourth drive motor 411 drives the fourth lead screw 412 to rotate, thereby creating a tendency to drive the fourth transmission component 413 to rotate circumferentially. Since the fourth transmission component 413 is circumferentially limited by the third plate 323, this rotational tendency is converted into a tendency to move along the axial direction of the fourth lead screw 412. It is easy to understand that when the output shaft of the fourth drive motor 411 switches between forward and reverse rotation, the corresponding direction of movement of the fourth transmission component 413 switches in the opposite direction.

[0133] Furthermore, such as Figure 5 As shown, the air blowing assembly 42 includes an air blowing bracket 421 and an air needle 422. The air blowing bracket 421 is connected to the fourth transmission member 413. The air inlet of the air blowing bracket 421 is connected to an external air source (not shown in the figure) through an air tube. One end of the air needle 422 is connected to the air outlet of the air blowing bracket 421, and the other end of the air needle 422 is used to extend into the reagent kit 100. Exemplarily, the external air source can be a gas cylinder or an air pump.

[0134] In some examples, the fourth drive mechanism 41 also includes a fourth slider 415.

[0135] See Figure 5 And refer to Figure 7 The fourth slider 415 is located above the third slider 33352. The fourth slider 415 is slidably connected to the third slide rail 33351, and the fourth slider 415 is connected to the air blowing bracket 421 and the fourth transmission component 413 respectively.

[0136] This improves the vertical stability of the air blowing assembly 42.

[0137] In some possible embodiments, see Figure 4 and Figure 5 The magnetic rod sleeve assembly 332 includes multiple magnetic rod sleeves, the magnetic rod assembly 334 includes multiple magnetic rods, and the air blowing assembly 42 includes multiple air needles 422. The number of magnetic rod sleeves, magnetic rods, and air needles 422 are the same in the magnetic bead biological sample extraction device.

[0138] In this way, each magnetic rod sleeve, each magnetic rod, and each air needle 422 can perform extraction and concentration steps on a biological sample in a kit 100, respectively, thereby improving efficiency.

[0139] For example, see Figure 4 and Figure 5 The magnetic rod sleeve assembly 332 includes twelve magnetic rod sleeves, the magnetic rod assembly 334 includes twelve magnetic rods, and the air blowing assembly 42 includes twelve air needles 422.

[0140] For example, see Figure 4 and Figure 5 The aforementioned magnetic rod sleeve, magnetic rod, and air needle 422 can all be arranged in a 2×6 matrix. See also Figure 2 The magnetic bead-based biological sample extraction device includes two reagent kit holders 2, each with six reagent kit compartments.

[0141] In some possible embodiments, such as Figure 6 As shown, the concentration mechanism 4 also includes multiple ventilation fans 43, for reference. Figure 1 The cover 7 has a ventilation area 71, which is composed of a plurality of spaced through holes, and each ventilation fan 43 is arranged opposite to a ventilation area 71.

[0142] In practice, multiple ventilation fans 43 are used for internal ventilation of the magnetic bead-based biological sample extraction device. In some examples, the concentration unit 4 includes two ventilation fans 43 located on either side of the reagent kit holder 2, forming a gas flow channel through the reagent kit holder 2.

[0143] For example, see Figure 6 The magnetic bead-based biological sample extraction device also includes a mounting frame 8 connected to the base 1. The mounting frame 8 has opposing first and second walls. The concentration mechanism 4 includes two ventilation fans 43, one fan 43 disposed on the first wall and the other fan 43 disposed on the second wall. A gas flow channel formed between the two fans 43 passes through the reagent kit. See also Figure 1 The cover 7 is provided with a ventilation hole at the position corresponding to the ventilation fan 43.

[0144] In this way, by setting up a ventilation fan 43, the evaporated solvent gas can be quickly discharged to the outside of the cover 7, and ventilation can be achieved inside the magnetic bead biological sample extraction device.

[0145] In some possible embodiments, see Figure 2 The magnetic bead-based biological sample extraction device also includes at least one cable chain 6, which is used to house the cable of the drive motor.

[0146] For example, the magnetic bead biological sample extraction device also includes three cable carriers 6, which respectively accommodate the cables of the second drive motor 3311, the third drive motor 3332 and the fourth drive motor 411.

[0147] The technical solutions provided in this disclosure have at least the following beneficial effects:

[0148] This disclosure provides a magnetic bead-based biological sample extraction device that integrates an extraction mechanism 3 and a concentration mechanism 4. The magnetic bead-based biological sample extraction device can complete the extraction and concentration steps separately. Compared with related technologies, the concentration step can be completed without manual transfer after the extraction step, reducing the risk of contamination.

[0149] The terminology used in the embodiments of this disclosure is for illustrative purposes only and is not intended to limit the disclosure. Unless otherwise defined, the technical or scientific terms used herein should be understood in their ordinary sense by one of ordinary skill in the art to which this disclosure pertains. The terms “first,” “second,” “third,” and similar terms used in this patent application specification and claims do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Similarly, the terms “a” or “one,” and similar terms do not indicate a quantity limitation, but rather indicate the presence of at least one. The terms “comprising,” “including,” and similar terms mean that the elements or objects preceding “comprising” or “including” encompass the elements or objects listed following “comprising” or “including” and their equivalents, and do not exclude other elements or objects. The terms “connected,” “linked,” and similar terms are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. The terms “upper,” “lower,” “left,” “right,” etc., are used only to indicate relative positional relationships, and these relative positional relationships may change accordingly when the absolute position of the described object changes.

[0150] The above description is merely an optional embodiment of this disclosure and is not intended to limit this disclosure. Any modifications, equivalent substitutions, improvements, etc., made within the principles of this disclosure should be included within the protection scope of this disclosure.

Claims

1. A magnetic bead method biological sample extraction apparatus characterized by comprising: The magnetic bead-based biological sample extraction device includes a base (1), a reagent kit holder (2), an extraction mechanism (3), and a concentration mechanism (4); The reagent kit holder (2) is connected to the base (1), and the reagent kit holder (2) is used to accommodate the reagent kit (100); The extraction mechanism (3) is connected to the base (1) by a transmission. The extraction mechanism (3) is used to extract biological samples and mix the biological samples with the solvent in the reagent kit (100). The concentration mechanism (4) is connected to the extraction mechanism (3) by transmission. The concentration mechanism (4) is used to deliver gas into the reagent kit (100) to evaporate the solvent.

2. The magnetic bead-based biological sample extraction apparatus according to claim 1, wherein The extraction mechanism (3) includes a first drive mechanism (31), a frame (32), and an extraction mechanism body (33); The first drive mechanism (31) is connected to the base (1) and is connected to the frame (32) in a transmission manner. The first drive mechanism (31) is used to drive the frame (32) to move in the horizontal direction. The extraction mechanism body (33) is connected to the frame (32).

3. The magnetic bead-based biological sample extraction device according to claim 2, characterized in that, The first drive mechanism (31) includes a first drive motor (311), a first lead screw (312), and a first transmission component (313); The first lead screw (312) extends horizontally and is connected to the output shaft of the first drive motor (311); The first transmission component (313) is circumferentially limited by the base (1), the first transmission component (313) is fitted outside the first lead screw (312) and connected to the frame (32).

4. The magnetic bead-based biological sample extraction device according to claim 2, characterized in that, The extraction mechanism body (33) includes a second drive mechanism (331), a magnetic rod sleeve assembly (332), a third drive mechanism (333), and a magnetic rod assembly (334); The second drive mechanism (331) is connected to the frame (32) and is connected to the magnetic rod sleeve assembly (332) in a transmission manner. The second drive mechanism (331) is used to drive the magnetic rod sleeve assembly (332) to move in the vertical direction. The third driving mechanism (333) is connected to the second driving mechanism (331) and is connected to the magnetic rod assembly (334) in a transmission manner. The third driving mechanism (333) is used to drive the magnetic rod assembly (334) to move in the vertical direction.

5. The magnetic bead-based biological sample extraction device according to claim 4, characterized in that, The second drive mechanism (331) includes a second drive motor (3311), a second lead screw (3312), and a second transmission member (3313). The second lead screw (3312) extends vertically and is connected to the output shaft of the second drive motor (3311). The second transmission member (3313) is circumferentially limited by the frame (32). The second transmission member (3313) is fitted outside the second lead screw (3312) and is connected to the magnetic rod sleeve assembly (332). The third drive mechanism (333) includes a connector (3331), a third drive motor (3332), a third lead screw (3333), and a third transmission component (3334). The connector (3331) is located on the side of the second transmission component (3313) away from the frame (32) and is connected to the second transmission component (3313). The third drive motor (3332) is connected to the connector (3331). The third lead screw (3333) extends vertically and is connected to the output shaft of the third drive motor (3332). The third transmission component (3334) is circumferentially limited by the connector (3331). The third transmission component (3334) is fitted outside the third lead screw (3333) and is connected to the magnetic rod assembly (334).

6. The magnetic bead-based biological sample extraction device according to claim 2, characterized in that, The concentration mechanism (4) includes a fourth drive mechanism (41) and an air blowing assembly (42); The fourth drive mechanism (41) is connected to the frame (32) and is connected to the air blowing assembly (42) in a transmission manner. The fourth drive mechanism (41) is used to drive the air blowing assembly (42) to move in the vertical direction.

7. The magnetic bead-based biological sample extraction device according to claim 6, characterized in that, The fourth drive mechanism (41) includes a fourth drive motor (411), a fourth lead screw (412), and a fourth transmission component (413); The fourth lead screw (412) extends vertically and is connected to the output shaft of the fourth drive motor (411); The fourth transmission component (413) is circumferentially limited by the frame (32), and the fourth transmission component (413) is fitted outside the fourth lead screw (412); The blowing assembly (42) includes a blowing bracket (421) and an air needle (422). The blowing bracket (421) is connected to the fourth transmission component (413). The air inlet of the blowing bracket (421) is connected to an external air source through an air pipe. One end of the air needle (422) is connected to the air outlet of the air blowing bracket (421), and the other end of the air needle (422) is used to extend into the reagent kit (100).

8. The magnetic bead-based biological sample extraction apparatus according to claim 1, wherein The concentration mechanism (4) also includes a plurality of ventilation fans (43) for ventilation of the interior of the magnetic bead biological sample extraction device.

9. The magnetic bead-based biological sample extraction device according to claim 1, characterized in that, The magnetic bead biological sample extraction device also includes a temperature control component (5), which includes a heating element (51), a temperature control fan (52), and a control unit; The heating element (51) is arranged opposite to the reagent kit holder (2); The temperature-controlled fan (52) is connected to the base (1) and is arranged opposite to the heating element (51); The control unit is electrically connected to the heating element (51) and the temperature control fan (52).

10. The magnetic bead-based biological sample extraction device according to any one of claims 1 to 9, characterized in that, The magnetic bead-based biological sample extraction device includes at least one cable chain (6) for accommodating the cable of a drive motor.

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