A multi-channel constant temperature amplification detection device

Abstract

This utility model relates to the field of isothermal amplification detection technology, and discloses a multi-channel isothermal amplification detection device, including a protective shell. A display screen is fixedly connected to the outside of the protective shell. An adjustment mechanism is provided inside the protective shell. An opening and closing component for opening and closing is rotatably connected to the outside of the protective shell. A sterilization mechanism is provided inside the protective shell. The adjustment mechanism includes a cylinder. A sliding plate is fixedly connected to the drive end of the cylinder. An automatic telescopic column is fixedly connected inside the sliding plate. A sample placement component is fixedly connected to the top of the automatic telescopic column. In this utility model, the cylinder and the automatic telescopic column move flexibly in conjunction with the fluorescence detection head to detect fluorescence changes during the amplification process. Because fluorescence signal changes occur during nucleic acid amplification, the height can be adjusted as needed to ensure that the sample is placed stably and does not shake and affect the detection.

Description

Technical Field

[0001] This utility model relates to the field of isothermal amplification and detection technology, and in particular to a multi-channel isothermal amplification and detection device. Background Technology

[0002] Against the backdrop of numerous challenges and opportunities facing global public health today, accurate and rapid medical testing technologies have become a crucial line of defense for safeguarding human health. With an aging population, frequent outbreaks of infectious diseases, and increasing public awareness of their health, the demand for high-efficiency testing equipment in the medical diagnostics field has exploded. Multi-channel isothermal amplification testing equipment has emerged to address the urgent need for rapid screening of large numbers of samples in various complex scenarios. Whether it's the busy laboratories of large hospitals, the emergency testing in disease control centers responding to sudden outbreaks, or the preliminary diagnosis of common diseases at grassroots medical sites in remote areas, the high accuracy and high throughput capabilities provided by this type of equipment are indispensable.

[0003] Nucleic acid detection technologies are mostly based on the principle of polymerase chain reaction with thermal cycling. By repeatedly raising and lowering the temperature, the denaturation, annealing and extension processes of nucleic acids are achieved, thereby amplifying the target nucleic acid sequence. This also puts forward requirements on the equipment hardware.

[0004] In existing technologies, some devices have only one detection site and can only detect a single sample. To address this issue, a multi-channel isothermal amplification detection device is proposed. Utility Model Content

[0005] To overcome the above shortcomings, this utility model provides a multi-channel isothermal amplification detection device, which aims to improve the problem of single detection in some existing devices.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a multi-channel isothermal amplification detection device, comprising a protective shell, a display screen fixedly connected to the outside of the protective shell, an adjustment mechanism provided inside the protective shell, an opening and closing assembly rotatably connected to the outside of the protective shell, a sterilization mechanism provided inside the protective shell, the adjustment mechanism comprising a cylinder, the cylinder fixedly connected to the outside of the protective shell, a sliding plate fixedly connected to the drive end of the cylinder, an automatic telescopic column fixedly connected inside the sliding plate, and a sample placement assembly fixedly connected to the top of the automatic telescopic column;

[0007] As a further description of the above technical solution: the disinfection mechanism includes a disinfection head, and the outside of the disinfection head is fixedly connected to the inside of the protective shell;

[0008] As a further description of the above technical solution: the placement mechanism includes four connecting plates, the connecting plates are fixedly connected to the outside of the automatic telescopic column, and six detection plates are fixedly connected to the outside of the connecting plates;

[0009] As a further description of the above technical solution: a supporting base plate is fixedly connected to the bottom of the protective shell, and a plurality of sliding strips are fixedly connected to the top of the supporting base plate;

[0010] As a further description of the above technical solution: the outer side of the sliding bar is slidably connected to the inside of the sliding plate, and the outer side of the sliding plate is slidably connected to the outside of the supporting base plate;

[0011] As a further description of the above technical solution: the opening and closing component includes a magnetic suction piece, the magnetic suction piece is fixedly connected to the outside of the protective shell, and the protective shell is rotatably connected to an opening cover.

[0012] As a further description of the above technical solution: a suction plate is fixedly connected to the outside of the opening, and two magnetic suction plates are fixedly connected to the outside of the suction plate;

[0013] As a further description of the above technical solution: a constant temperature controller is fixedly connected inside the protective shell, and a fluorescent detection head is fixedly connected to the top side inside the protective shell.

[0014] This utility model has the following beneficial effects:

[0015] 1. In this utility model, a cylinder pushes a sliding plate to slide onto a sliding bar. The sliding bar is fixedly supported on the base plate and can also slide inside the sliding plate. At the same time, an automatic telescopic column is connected to a sample placement component. Six detection plates are connected to the connecting plate. The cylinder and the automatic telescopic column move flexibly in coordination, and work with the fluorescence detection head to detect fluorescence changes during the amplification process. Because fluorescence signal changes are generated during nucleic acid amplification, the height can be adjusted as needed to ensure that the sample is placed stably and will not shake and affect the detection.

[0016] 2. In this utility model, the disinfection head automatically starts after each batch of testing is completed, spraying disinfectant to thoroughly clean the internal contaminants such as nucleic acid and bacteria, creating a clean and pollution-free environment for the next batch of testing, effectively preventing cross-contamination of samples, ensuring the consistency and reliability of test results for multiple consecutive batches, and reducing the probability of false positive or false negative results, regardless of the intensive testing scenario in the scientific research laboratory. Attached Figure Description

[0017] Figure 1 This is a three-dimensional schematic diagram of a multi-channel isothermal amplification detection device proposed in this utility model;

[0018] Figure 2 This is a schematic diagram of the connecting plate of a multi-channel isothermal amplification detection device proposed in this utility model;

[0019] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0020] Figure 4 This is a schematic diagram of the sterilization head of a multi-channel isothermal amplification detection device proposed in this utility model.

[0021] Legend:

[0022] 1. Protective shell; 2. Display screen; 3. Magnetic suction plate one; 4. Magnetic suction plate two; 5. Opening cover; 6. Suction plate; 7. Detection plate; 8. Sliding plate; 9. Connecting plate; 10. Support base plate; 11. Sliding bar; 12. Fluorescent detection head; 13. Automatic telescopic column; 14. Thermostat; 15. Cylinder; 16. Sterilization head. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Reference Figures 1 to 3 The present invention provides an embodiment of a multi-channel isothermal amplification detection device, including a protective shell 1. The protective shell 1 is used to isolate external dust, moisture and other adverse factors to ensure stable operation of the device. A display screen 2 is fixedly connected to the outside of the protective shell 1. The display screen 2 intuitively displays the device's operating status, detection parameters and final results and other key information, allowing operators to monitor the detection dynamics at any time. An adjustment mechanism is provided inside the protective shell 1. An opening and closing component for opening and closing is rotatably connected to the outside of the protective shell 1. A disinfection mechanism is provided inside the protective shell 1.

[0025] The adjustment mechanism includes a cylinder 15, which is externally and fixedly connected to the inside of the protective shell 1. A sliding plate 8 is fixedly connected to the drive end of the cylinder 15. The cylinder 15 is used to push the sliding plate 8 to slide, allowing for flexible adjustment of the sample placement position. The sliding plate 8 moves smoothly under the drive of the cylinder 15 and the guidance of the sliding strip 11. An automatic telescopic column 13 is fixedly connected inside the sliding plate 8. The automatic telescopic column 13 can be flexibly extended and retracted as needed to easily adapt to different height requirements, ensuring stable sample placement and effectively avoiding the impact of improper placement on the detection process. A sample placement component is fixedly connected to the top of the automatic telescopic column 13. The placement mechanism includes four connecting plates 9, which transmit and distribute power, ensuring that the six detection plates 7 work together to realize multi-channel sample... The connecting plate 9 is externally fixedly connected to the outside of the automatic telescopic column 13. There are a total of twenty-four detection plates 7, which are evenly distributed on the connecting plate 9 to provide a dedicated placement position for the sample to be tested. Six detection plates 7 are fixedly connected to the outside of the connecting plate 9. The bottom of the protective shell 1 is fixedly connected to the support base plate 10. The support base plate 10 is used to support the entire internal mechanical structure. Multiple sliding strips 11 are fixedly connected to the top of the support base plate 10. The sliding strips 11 are fixed to the top of the support base plate 10, which not only have a tight sliding connection with the inside of the sliding plate 8, but also assist the sliding plate 8 to slide smoothly outside the support base plate 10, ensuring that the movement trajectory of the sliding plate 8 is correct. The outside of the sliding strips 11 is slidably connected to the inside of the sliding plate 8, and the outside of the sliding plate 8 is slidably connected to the outside of the support base plate 10.

[0026] Reference Figure 1 , Figure 2 and Figure 4 The disinfection mechanism includes a disinfection head 16. After each testing task, the disinfection head 16 promptly sprays disinfectant to disinfect any residual nucleic acid, bacteria, and other contaminants, preventing cross-contamination. The disinfection head 16 is externally and fixedly connected to the inside of the protective shell 1. The opening and closing assembly includes a magnetic suction plate 3, which works in tandem with a magnetic suction plate 4 on the cover 5 to securely lock the cover 5 in place using magnetic force. The cover 5 provides a convenient passage for operators. The magnetic suction plate 3 is externally and fixedly connected to the outside of the protective shell 1. The cover 5 is rotatably connected to the outside of the protective shell 1, and a suction plate 6 is fixedly connected to the outside of the cover 5. Plate 6 supports magnetic suction pads 4, providing a stable support structure for the magnetic closure mechanism. Two magnetic suction pads 4 are fixedly connected to the outside of the suction plate 6. A thermostat 14 is fixedly connected inside the protective shell 1. The thermostat 14 controls the internal temperature of the equipment throughout the process, maintaining a constant and suitable environment for nucleic acid amplification and ensuring the accuracy of the detection results. A fluorescence detection head 12 is fixedly connected to the top inside the protective shell 1. The fluorescence detection head 12 is located on the top inside the protective shell 1 and focuses intently on capturing changes in fluorescence signals during nucleic acid amplification. It uses this to determine the presence or absence of the target nucleic acid in the sample, providing a key basis for the determination of the detection results.

[0027] Working principle: The operator places the sample and flips the cover 5. The cover 5 rotates around the protective shell 1. A magnetic suction piece 3 is fixed on the outside of the protective shell 1. A suction plate 6 is connected to the outside of the cover 5. There are two magnetic suction pieces 4 on the suction plate 6. Through the attraction of magnetic suction pieces 3 and magnetic suction pieces 4, the cover 5 is tightly closed.

[0028] After opening the cover 5, the sample is placed in and then pushed by the cylinder 15 to slide the sliding plate 8 onto the sliding strip 11. The sliding strip 11 is fixed on the support base plate 10 and can also slide inside the sliding plate 8. At the same time, the automatic telescopic column 13, which is connected to the sample placement component, and the connecting plate 9, are connected to six detection plates 7. The cylinder 15 and the automatic telescopic column 13 work together to move flexibly and cooperate with the fluorescence detection head 12 to detect the fluorescence changes during the amplification process. Because the nucleic acid amplification will produce fluorescence signal changes, the temperature is kept constant by the thermostat 14. The fluorescence detection head 12 captures these changes and can identify the target nucleic acid of the sample. The data is transmitted to the display screen 2 and the height can be adjusted as needed to ensure that the sample is placed stably and will not shake and affect the detection.

[0029] After the testing is completed, the samples are taken out and passed through the disinfection head 16. After one batch of samples is tested, the disinfection head 16 is activated, spraying disinfectant to clean up any residual nucleic acid, bacteria, etc. in the equipment, so that the next batch of tests will not be disturbed.

[0030] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-channel isothermal amplification detection device, comprising a protective shell (1), characterized in that: The protective shell (1) is fixedly connected to the outside of a display screen (2), the protective shell (1) is provided with an adjustment mechanism inside, the protective shell (1) is rotatably connected to an opening and closing component for opening and closing, and the protective shell (1) is provided with a disinfection mechanism inside. The adjustment mechanism includes a cylinder (15), the outside of which is fixedly connected to the inside of the protective shell (1). A sliding plate (8) is fixedly connected to the drive end of the cylinder (15). An automatic telescopic column (13) is fixedly connected inside the sliding plate (8). A placement component for placing samples is fixedly connected to the top of the automatic telescopic column (13).

2. The multi-channel isothermal amplification detection device according to claim 1, characterized in that: The disinfection mechanism includes a disinfection head (16), which is externally fixedly connected to the inside of the protective shell (1).

3. The multi-channel isothermal amplification detection device according to claim 1, characterized in that: The placement assembly includes four connecting plates (9), which are externally fixedly connected to the outside of the automatic telescopic column (13), and six detection plates (7) are externally fixedly connected to the outside of the connecting plates (9).

4. The multi-channel isothermal amplification detection device according to claim 3, characterized in that: The bottom of the protective shell (1) is fixedly connected to a support base plate (10), and the top of the support base plate (10) is fixedly connected to a plurality of sliding strips (11).

5. The multi-channel isothermal amplification detection device according to claim 4, characterized in that: The sliding bar (11) is externally slidably connected to the inside of the sliding plate (8), and the sliding plate (8) is externally slidably connected to the outside of the supporting base plate (10).

6. The multi-channel isothermal amplification detection device according to claim 1, characterized in that: The opening and closing assembly includes a magnetic suction piece (3), which is fixedly connected to the outside of the protective shell (1), and the protective shell (1) is rotatably connected to an opening cover (5).

7. The multi-channel isothermal amplification detection device according to claim 6, characterized in that: The opening cover (5) is fixedly connected to the outside of a suction plate (6), and the suction plate (6) is fixedly connected to two magnetic suction pieces (4).

8. The multi-channel isothermal amplification detection device according to claim 1, characterized in that: A thermostat (14) is fixedly connected inside the protective shell (1), and a fluorescent detection head (12) is fixedly connected to the top side inside the protective shell (1).

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