Adapting a multi-detection platform for ao / pi cell viability assay kit

Abstract

This utility model discloses an AO / PI cell viability assay kit adapted to multiple detection platforms, relating to the field of cell viability assay technology. The AO / PI cell viability assay kit includes a shell, a sealing door rotatably connected to the top of the shell, a sealing ring fixedly installed on the sealing door, a power box fixedly installed on the top of the shell, a servo motor fixedly installed at the bottom of the power box, a drive sprocket fixedly installed on the output shaft of the servo motor, a rotating shaft rotatably installed inside the power box, a driven sprocket fixedly installed at the upper end of the rotating shaft, and a chain drive connecting the drive sprocket and the driven sprocket. The servo motor inside the power box drives the rotating shaft to rotate smoothly through the drive sprocket, chain, and driven sprocket, thereby causing the test tube rack and mixing tube to rotate synchronously to achieve centrifugal mixing. This ensures that the cell sample in the mixing tube is fully and evenly contacted with the AO solution, PI solution, and buffer solution, avoiding detection errors caused by uneven mixing and improving detection accuracy.

Description

Technical Field

[0001] This utility model relates to the field of cell viability detection technology, and in particular to an AO / PI cell viability detection kit adapted to multiple detection platforms. Background Technology

[0002] In the current field of cell viability testing, existing testing solutions generally have many technical defects, making it difficult to meet the needs of efficient, accurate, and stable testing. First, existing solutions lack effective environmental protection design, and a closed testing space cannot be formed during the testing process. Dust and impurities in the external environment can easily come into direct contact with samples and reagents, leading to sample contamination. At the same time, AO reagents, PI reagents, and buffer solutions are easily affected by external factors during storage and use, causing the reagent concentration to deviate from the standard range. This not only damages the stability of the reagents themselves but also directly affects the effect of subsequent staining reactions, resulting in deviations in test results. The cleanliness of the testing environment and the stability of the reagents cannot be guaranteed. Secondly, in the sample and reagent mixing process, existing methods mostly rely on manual shaking or simple oscillation to achieve mixing. This mixing method is difficult to ensure that the sample is fully contacted and uniformly mixed with AO reagent, PI reagent, and buffer solution. It is easy to have local uneven mixing. Uneven mixing will result in insufficient cell staining in some areas and over-staining in other areas, making it impossible to accurately distinguish between live cells, dead cells, and apoptotic cells during subsequent observation. This seriously affects the accuracy of the test results and fails to meet the core requirement of uniformity of the mixture in the test. Furthermore, the existing methods lack precision in controlling sample addition and reagent release. It is difficult to accurately align the sample with the reaction container when adding it, which can easily lead to sample spillage and waste of precious samples. Moreover, the release of AO reagents, PI reagents and buffer solutions is mostly controlled manually, which makes it impossible to accurately control the timing and dosage of reagent release. This can easily lead to excessive consumption of reagents and may also cause premature contact and reaction between different reagents, resulting in reagent failure or the generation of contaminants, which can further interfere with the detection process and increase the risk of detection failure. Finally, the existing solution has extremely low functional integration. Sampling, sample addition, and mixing operations all require separate equipment, making the detection process cumbersome. Operators need to frequently manually transfer samples and reagents, which not only increases operational complexity and prolongs the overall detection time, but also reduces detection efficiency. Furthermore, due to differences in the operating standards and adaptation requirements of different equipment, it is difficult to quickly and smoothly switch to different detection platforms such as fluorescence microscopy and flow cytometers, thus failing to meet the demand for efficient and convenient detection in diverse detection scenarios. Utility Model Content

[0003] The purpose of this invention is to at least solve one of the technical problems existing in the prior art, and to provide an AO / PI cell viability assay kit that is compatible with multiple detection platforms. This kit can solve the problem of insufficient mixing of samples and reagents, avoid uneven mixing caused by manual shaking or simple oscillation, ensure that cell samples are in full contact with AO reagents, PI reagents and buffer solutions and are mixed evenly, reduce detection errors caused by mixing defects, improve detection accuracy, and solve the problem of low precision in sample addition and reagent release.

[0004] To achieve the above objectives, this utility model provides the following technical solution: An AO / PI cell viability assay kit compatible with multiple detection platforms includes a housing, a sealed door rotatably connected to the top of the housing, a sealing ring fixedly installed on the sealed door, a power box fixedly installed on the top of the housing, a servo motor fixedly installed at the bottom of the power box, a drive sprocket fixedly installed on the output shaft of the servo motor, a rotating shaft rotatably installed inside the power box, a driven sprocket fixedly installed at the upper end of the rotating shaft, the drive sprocket and the driven sprocket being connected by chain drive, the lower end of the rotating shaft penetrating the top of the housing and rotatably installed inside the housing, and a bearing is provided at the penetration point between the rotating shaft and the housing; An inner liner is fixedly installed inside the outer shell. The inner liner is annular and surrounds the outside of the test tube rack. A positioning sensor is built into the inner liner. A gap is left between the inner wall of the inner liner and the outside of the test tube rack. The test tube rack is fixedly installed on the part of the rotating shaft located inside the inner liner. Multiple positioning slots are provided on the test tube rack, and a mixing tube is placed in the positioning slot. A sampling needle is fixedly installed on the top of the outer shell. The lower end of the sampling needle penetrates the top of the outer shell and extends into the interior of the outer shell. The lower end of the sampling needle is positioned corresponding to the opening of the mixing tube below. A multi-chamber storage chamber is fixedly installed on the top of the outer shell. The storage chamber has an AO solution outlet, a PI solution outlet, and a buffer solution outlet. A solenoid valve is fixedly installed at each of the AO solution outlet, PI solution outlet, and buffer solution outlet. The outlet end of the solenoid valve is connected to a pipe. The lower end of the pipe penetrates the top of the outer shell and extends into the interior of the outer shell. The end of the pipe is positioned corresponding to the opening of the mixing tube below.

[0005] Preferably, a shock absorber is fitted on the outside of the servo motor, and multiple shock absorber springs are fixedly installed between the inner wall of the shock absorber and the outer wall of the servo motor. The bottom of the shock absorber is fixedly installed at the bottom of the power box.

[0006] Preferably, the positioning sensor built into the inner liner is an infrared positioning sensor, and the number of infrared positioning sensors is the same as the number of positioning slots on the test tube rack, with each infrared positioning sensor corresponding to one positioning slot.

[0007] Preferably, an anti-slip silicone pad is fixedly installed on the inner wall of the positioning groove on the test tube rack, and the surface of the anti-slip silicone pad has multiple anti-slip patterns.

[0008] Preferably, each chamber of the storage compartment has graduation lines on its outer side, and the storage compartment is made of transparent polycarbonate material.

[0009] Preferably, the pipe of the solenoid valve is a flexible tube made of polytetrafluoroethylene, and a guide nozzle is fixedly installed at the end of the pipe, and the outlet end of the guide nozzle is set at an angle of °.

[0010] Preferably, a shock-absorbing pad is fixedly installed at the bottom inside the outer shell. The shock-absorbing pad is made of nitrile rubber and is positioned above the bottom of the inner liner.

[0011] Preferably, a protective sleeve is provided on the outside of the sampling needle, the protective sleeve is fixedly connected to the top of the outer shell, the protective sleeve is made of stainless steel, and a gap is left between the inner wall of the protective sleeve and the outer wall of the sampling needle.

[0012] Compared with the prior art, the beneficial effects of this utility model are: (1) The AO / PI cell viability assay kit, which is compatible with multiple detection platforms, has an outer shell that provides stable support and protection for the device. When the sealed door is closed, it forms a closed cavity with the sealing ring, which can prevent external impurities from entering and contaminating the sample and reagents, and also avoid the evaporation of reagents such as AO solution and PI solution from affecting the concentration, thus ensuring the cleanliness of the detection environment and the stability of the reagents. The servo motor in the power box drives the rotating shaft to rotate smoothly through the active sprocket, chain, and driven sprocket, thereby enabling the test tube rack and mixing tube to rotate synchronously to achieve centrifugal mixing. This allows the cell sample in the mixing tube to fully and evenly contact the AO solution, PI solution, and buffer solution, avoiding detection errors caused by uneven mixing and improving detection accuracy.

[0013] (2) The AO / PI cell viability assay kit, which is compatible with multiple detection platforms, has a built-in positioning sensor in the inner liner to accurately monitor the position of the test tube rack. This allows the mixing tube to be accurately aligned with the sampling needle for sample addition and to be accurately aligned with the corresponding pipes of the AO solution outlet, PI solution outlet, and buffer solution outlet in the storage chamber. Combined with the solenoid valve, it can accurately control the release of each reagent, avoiding reagent waste and preventing contamination caused by premature mixing of different reagents. The positioning groove of the test tube rack can fix the mixing tube and prevent it from shaking and tipping over during centrifugation. The multi-chamber design of the storage chamber allows for independent storage of the three reagents, further reducing the risk of contamination. The whole device integrates sampling, sample addition, and mixing functions, making it easy to operate. After mixing, the mixing tube can be easily removed to adapt to multiple detection platforms, effectively simplifying the detection process and improving detection efficiency and reliability. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments: Figure 1 This is a schematic diagram of the AO / PI cell viability assay kit adapted to multiple detection platforms according to this utility model; Figure 2 This is a schematic diagram of the AO / PI cell viability assay kit adapted to multiple detection platforms according to this utility model; Figure 3 This is a cross-sectional schematic diagram of the AO / PI cell viability assay kit adapted to multiple detection platforms according to this utility model; Figure 4 This is a cross-sectional schematic diagram of the AO / PI cell viability assay kit adapted to multiple detection platforms according to this utility model.

[0015] Reference numerals: 1. Outer shell; 2. Sealing door; 3. Sealing ring; 4. Sampling needle; 5. Rotating shaft; 6. Power box; 7. Servo motor; 8. Storage chamber; 9. AO solution outlet; 10. Solenoid valve; 11. PI solution outlet; 12. Buffer solution outlet; 13. Mixing tube; 14. Test tube rack; 15. Inner liner; 16. Driven sprocket; 17. Driven sprocket; 18. Chain. Detailed Implementation

[0016] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.

[0017] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0018] In the description of this utility model, terms such as greater than, less than, and exceeding are understood to exclude the stated number, while terms such as above, below, and within are understood to include the stated number. The use of terms like "first" and "second" is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the quantity or sequence of the indicated technical features.

[0019] In the description of this utility model, unless otherwise explicitly defined, terms such as "setting," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this utility model in conjunction with the specific content of the technical solution.

[0020] Please see Figure 1-4 This utility model provides a technical solution: an AO / PI cell viability test kit adapted to multiple detection platforms, including a shell 1, a sealing door 2 rotatably connected to the top of the shell 1, a sealing ring 3 fixedly installed on the sealing door 2, a power box 6 fixedly installed on the top of the shell 1, a servo motor 7 fixedly installed at the bottom of the power box 6, a drive sprocket 17 fixedly installed on the output shaft of the servo motor 7, a rotating shaft 5 rotatably installed inside the power box 6, a driven sprocket 16 fixedly installed at the upper end of the rotating shaft 5, the drive sprocket 17 and the driven sprocket 16 are connected by a chain 18, the lower end of the rotating shaft 5 passes through the top of the shell 1 and is rotatably installed inside the shell 1, and a bearing is provided at the penetration point between the rotating shaft 5 and the shell 1; The outer shell 1 serves as the core support structure of the reagent kit, providing an installation base and protective space for all internal functional components, thus preventing interference from the external environment to the detection process. The sealing door 2 is connected to the outer shell 1 by a rotating connection. Before detection, the sealing door 2 can be opened to take out or put in the mixing tube 13 or to debug the equipment. During the detection process, the sealing door 2 is closed. At this time, the sealing ring 3 on the sealing door 2 will fit tightly with the top edge of the outer shell 1 to form a closed cavity, preventing the internal reagents from evaporating or external impurities from entering, and ensuring a clean detection environment. The power box 6 is fixed above the outer shell 1. The servo motor 7 inside is the core power source for power output and centrifugal mixing. When it is necessary to mix reagents and samples or adjust the position of the test tube rack 14, the servo motor 7 is started. Its output shaft drives the active sprocket 17 to rotate synchronously. The active sprocket 17 transmits power to the driven sprocket 16 through the chain 18. Since the driven sprocket 16 is fixedly connected to the upper end of the rotating shaft 5, it drives the rotating shaft 5 to rotate. The lower end of the rotating shaft 5 passes through the top of the outer shell 1 and is installed inside the outer shell 1 through a bearing. The bearing reduces the frictional resistance between the rotating shaft 5 and the outer shell 1 when rotating, ensuring that the rotating shaft 5 rotates smoothly. At the same time, the rotation of the rotating shaft 5 will drive the test tube rack 14 and the mixing tube 13 connected below to rotate synchronously. The centrifugal force generated by the rotation is used to achieve centrifugal mixing of the liquid in the mixing tube 13, so that the sample and reagent are fully contacted and uniformly mixed, meeting the requirements of uniformity of the mixture for subsequent detection. An inner liner 15 is fixedly installed inside the outer shell 1. The inner liner 15 is annular and surrounds the outside of the test tube rack 14 and has a positioning sensor built in it. There is a gap between the inner wall of the inner liner 15 and the outside of the test tube rack 14. The test tube rack 14 is fixedly installed on the part of the rotating shaft 5 located inside the inner liner 15. Multiple positioning slots are provided on the test tube rack 14, and a mixing tube 13 is placed in the positioning slot. The inner liner 15, fixed inside the outer shell 1, has a ring-shaped structure and surrounds the outside of the test tube rack 14. On the one hand, it can provide inner protection for the test tube rack 14 and the mixing tube 13, preventing impurities that may exist inside the outer shell 1 from directly contacting the mixing tube 13 and ensuring the cleanliness of the samples and reagents. On the other hand, the positioning sensor built into the inner liner 15 can monitor the position of the positioning slot on the test tube rack 14 in real time, providing signal support for the accurate positioning of the test tube rack 14. There is a gap between the inner wall of the inner liner 15 and the outer side of the test tube rack 14. This gap can prevent friction interference between the test tube rack 14 and the inner liner 15 during the rotation with the rotating shaft 5, ensuring that the test tube rack 14 rotates smoothly and does not affect the centrifugation mixing effect. The test tube rack 14 is fixed to the part of the rotating shaft 5 located inside the inner liner 15 and rotates synchronously with the rotating shaft 5. Multiple positioning slots on the test tube rack 14 are used to place the mixing tube 13. Through the structural limiting effect of the positioning slots, the mixing tube 13 can be prevented from shaking or tipping during centrifugal rotation or testing, ensuring that the mixing tube 13 is stably in the position required for centrifugal mixing. When the positioning sensor detects that a certain positioning slot on the test tube rack 14 has rotated to the target position, it will send a signal to the control terminal to stop or adjust the speed of the servo motor 7, so that the mixing tube 13 is accurately stopped in the corresponding position, providing accurate positioning for subsequent sampling and sample addition operations, while ensuring that the mixing tube 13 is always on a stable centrifugal trajectory during centrifugal mixing. A sampling needle 4 is fixedly installed on the top of the outer shell 1. The lower end of the sampling needle 4 penetrates the top of the outer shell 1 and extends into the interior of the outer shell 1. The lower end of the sampling needle 4 is set to correspond to the opening of the mixing tube 13 below. A multi-chamber storage chamber 8 is fixedly installed on the top of the outer shell 1. The storage chamber 8 is respectively provided with an AO solution outlet 9, a PI solution outlet 11 and a buffer solution outlet 12. A solenoid valve 10 is fixedly installed at each of the three outlets. The outlet end of the solenoid valve 10 is connected to a pipe. The lower end of the pipe penetrates the top of the outer shell 1 and extends into the interior of the outer shell 1. The end of the pipe is set to correspond to the opening of the mixing tube 13 below. The sampling needle 4 fixed on the top of the outer shell 1 is the cell sample delivery channel. When the test tube rack 14, under the action of the positioning sensor, drives a certain mixing tube 13 to stop precisely below the sampling needle 4, the lower end of the sampling needle 4 is aligned with the opening of the mixing tube 13, and the cell sample to be tested is delivered into the mixing tube 13, completing the initial addition of the sample. The multi-chamber storage chamber 8 on the upper part of the outer shell 1 is divided into three independent chambers, which are used to store AO solution, PI solution and buffer solution respectively, so as to achieve independent storage of the three reagents and avoid mixing and contamination during storage. The AO solution outlet 9, PI solution outlet 11 and buffer solution outlet 12 on the storage chamber 8 correspond to the release channels of the three reagents respectively. The solenoid valve 10 at each outlet can be controlled by a control signal to achieve precise opening and closing of the valve. When the mixing tube 13 stops below the pipe corresponding to a certain reagent, the solenoid valve 10 at the outlet of the corresponding reagent opens, and the reagent enters the connected pipe through the solenoid valve 10. The lower end of the pipe extends into the inner part of the outer shell 1 and the end is aligned with the opening of the mixing tube 13, so as to inject the reagent into the mixing tube 13 in a directional manner. After the cell sample, AO solution, PI solution, and buffer solution are injected into the mixing tube 13 in sequence, the servo motor 7 starts again, driving the rotating shaft 5, test tube rack 14, and mixing tube 13 to rotate. Centrifugal force is used to fully mix the sample and reagents in the mixing tube 13 to form a uniform detection mixture. After mixing is completed, the servo motor 7 stops, and the test tube rack 14 stops at the designated position under the action of the positioning sensor, which facilitates the subsequent removal of the mixing tube 13. It is compatible with multiple detection platforms such as fluorescence microscopes and flow cytometers for cell viability detection.

[0021] Working principle: When in use, open the sealing door 2, place the mixing tube 13 into the positioning slot of the test tube rack 14, close the sealing door 2 so that the sealing ring 3 fits with the outer shell 1 to form a closed cavity, the inner liner 15 has a built-in positioning sensor to stop the test tube rack 14 below the sampling needle 4, the sampling needle 4 injects cell samples into the mixing tube 13, and then the positioning makes the mixing tube 13 align with the corresponding pipes of the storage chamber 8: AO solution outlet 9, PI solution outlet 11, and buffer solution outlet 12. The solenoid valve 10 controls the injection of the three reagents into the mixing tube 13 respectively. The servo motor 7 starts, and drives the rotating shaft 5 through the active sprocket 17, chain 18, and driven sprocket 16 to rotate the test tube rack 14 and the mixing tube 13 to achieve centrifugal mixing. After mixing is completed, the positioning sensor makes the test tube rack 14 stop, open the sealing door 2 to take out the mixing tube 13, and adapt to multiple detection platforms for cell viability detection; The outer shell 1 provides stable support and protection for the device. When the sealing door 2 is closed, it works with the sealing ring 3 to form a closed cavity, which can prevent external impurities from entering and contaminating the sample and reagents, and also prevent the evaporation of reagents such as AO solution and PI solution from affecting the concentration, thus ensuring the cleanliness of the detection environment and the stability of the reagents. The servo motor 7 in the power box 6 drives the rotating shaft 5 to rotate smoothly through the active sprocket 17, chain 18, and driven sprocket 16, thereby making the test tube rack 14 and the mixing tube 13 rotate synchronously to achieve centrifugal mixing. This allows the cell sample in the mixing tube 13 to fully and evenly contact the AO solution, PI solution, and buffer solution, avoiding detection errors caused by uneven mixing and improving detection accuracy. The inner liner 15 has a built-in positioning sensor that can accurately monitor the position of the test tube rack 14, enabling the mixing tube 13 to be accurately aligned with the sampling needle 4 for sample addition. It can also be accurately aligned with the corresponding pipes of the AO solution outlet 9, PI solution outlet 11, and buffer solution outlet 12 in the storage chamber 8. With the help of the solenoid valve 10, it can accurately control the release of each reagent, avoiding reagent waste and preventing contamination caused by premature mixing of different reagents. The positioning groove of the test tube rack 14 can fix the mixing tube 13 to prevent it from shaking and tipping over during centrifugation. The multi-chamber design of the storage chamber 8 allows for independent storage of the three reagents, further reducing the risk of contamination. The whole device integrates sampling, sample addition, and mixing functions, making it easy to operate. After mixing, the mixing tube 13 can be easily removed to adapt to multiple detection platforms, effectively simplifying the detection process and improving detection efficiency and reliability.

[0022] Structural Description: Outer shell 1: The core support structure of the reagent kit. It is a closed box-shaped structure that provides a mounting base and protective space for all internal functional components. It is located on the outermost layer of the reagent kit to avoid interference from the external environment (such as impurities and temperature fluctuations) on the detection process and ensure the stability of the detection environment. Sealing door 2: A door structure that can rotate around the outer shell 1, located above the outer shell 1, and is connected to the edge of the outer shell 1 by a rotating connection. It can be rotated outward to open before testing, which is convenient for taking out and putting in the mixing tube 13 or for internal equipment debugging. During testing, it can be rotated inward to close, forming a closed structure with the top of the outer shell 1, providing a basis for subsequent sealing and protection. Sealing ring 3: An elastic annular sealing component, fixedly installed on the inner edge of the sealing door 2. When the sealing door 2 is closed, the sealing ring 3 will fit tightly against the top edge of the outer shell 1, filling the gap between the sealing door 2 and the outer shell 1 to form a closed cavity, preventing the internal reagent from evaporating and causing concentration changes, while blocking external impurities from entering and ensuring a clean testing environment. Sampling needle 4: A hollow tubular sample delivery channel, fixedly installed above the outer shell 1. Its lower end vertically penetrates the top of the outer shell 1 and extends into the interior of the outer shell 1. The opening at the lower end precisely corresponds to the opening of the mixing tube 13 on the test tube rack 14 below. When the mixing tube 13 stops directly below the sampling needle 4 under the action of the positioning sensor, the sampling needle 4 can directionally deliver the cell sample to be tested into the mixing tube 13, completing the initial addition of the sample. Rotating shaft 5: A cylindrical drive shaft, located inside the power box 6 and rotatably mounted via bearings. Its lower end vertically penetrates the top of the outer shell 1 and extends into the interior of the outer shell 1, with a bearing installed at the penetration point with the outer shell 1. Its upper end is fixedly connected to the driven sprocket 16, and its lower end is fixedly connected to the test tube rack 14. It can receive the power transmitted by the driven sprocket 16 and drive the test tube rack 14 to rotate synchronously. The bearings reduce the frictional resistance between the rotating shaft 5 and the outer shell 1 when rotating, ensuring that the rotating shaft 5 rotates smoothly and avoiding the impact of shaking on the mixing effect or positioning accuracy. Power box 6: Rectangular box structure, fixedly installed on the upper surface of the outer shell 1, forming an enclosed space inside to accommodate servo motor 7, drive sprocket 17, driven sprocket 16 and chain 18. It can protect the internal power transmission components, prevent external dust and moisture from affecting the operation of the components, and prevent the components from being exposed during power transmission, thus preventing safety hazards. 7. Servo motor 7: Power output device, fixedly installed at the bottom of the power box 6, with the output shaft extending upward and fixedly connected to the drive sprocket 17. It is the core power source for the reagent kit to realize power output and centrifugal mixing. When it is necessary to adjust the position of the test tube rack 14 or to perform centrifugal mixing, the servo motor 7 starts and drives the drive sprocket 17 to rotate through the output shaft, providing power support for subsequent power transmission and centrifugation. Storage compartment 8: A multi-chamber transparent storage structure, fixedly installed on the upper surface of the outer shell 1. The interior is divided into three independent sealed chambers by partitions, which are used to store AO solution, PI solution and buffer solution respectively. The transparent material makes it easy to observe the remaining amount of reagents inside. The independent chamber design can achieve isolated storage of the three reagents and avoid contamination or failure due to reagent mixing during storage. AO solution outlet 9: A circular channel opening located at the bottom of the AO solution chamber corresponding to the storage compartment 8. It is a dedicated release channel for the AO solution. A solenoid valve 10 is fixedly installed at the outlet. The release and cut-off of the AO solution can be controlled by opening and closing the solenoid valve 10 to ensure that the AO solution flows out precisely only when the mixing tube 13 is in place, avoiding reagent waste or accidental release. Solenoid valve 10: Solenoid-controlled valve, three in total, are fixedly installed at AO solution outlet 9, PI solution outlet 11 and buffer solution outlet 12 respectively. Each outlet is connected to a pipe. The valve can be opened and closed precisely by external control signal. When the mixing tube 13 stops below the corresponding reagent pipe, the corresponding solenoid valve 10 opens to allow the reagent to flow into the pipe, and closes otherwise, so as to realize independent control and precise regulation of the release of the three reagents. PI solution outlet 11: A circular channel opening located at the bottom of the PI solution chamber corresponding to the storage compartment 8. It is a dedicated release channel for the PI solution. A solenoid valve 10 is fixedly installed at the outlet, with the same function as the AO solution outlet 9. The opening and closing of the solenoid valve 10 controls the timing and dosage of the PI solution release, ensuring that the PI solution flows into the mixing tube 13 as needed. Buffer outlet 12: A circular channel opening located at the bottom of the buffer chamber corresponding to the storage compartment 8. It is a dedicated release channel for the buffer. A solenoid valve 10 is fixedly installed at the outlet. The release of the buffer is controlled by opening and closing the solenoid valve 10, so that the buffer flows into the mixing tube 13 after or simultaneously with the sample and AO and PI solutions are added, thereby adjusting the mixing system and ensuring the stable progress of the staining reaction. Mixing tube 13: A tubular container made of transparent and centrifugally resistant material. It is placed in the positioning slot of the test tube rack 14 to hold the cell sample to be tested, AO solution, PI solution, and buffer solution. It can rotate synchronously with the test tube rack 14 to achieve thorough mixing of the internal liquids under centrifugal force, forming a uniform detection mixture. At the same time, the transparent material makes it easy to remove and adapt to various detection platforms for observation and detection. Test tube rack 14: A circular rack structure, fixedly installed on the part of the rotating shaft 5 located inside the inner liner 15, radially distributed around the rotating shaft 5. Multiple evenly arranged positioning slots are opened on the rack, which are used to place the mixing tube 13. The slot structure limits the position of the mixing tube 13 to prevent it from shaking or tipping over during centrifugal rotation or testing. At the same time, it rotates synchronously with the rotating shaft 5, driving the mixing tube 13 to achieve centrifugal mixing. With the positioning sensor of the inner liner 15, it can achieve precise positioning and provide stable support for sampling and sample addition. Inner liner 15: A ring-shaped protective structure, fixedly installed inside the outer shell 1, surrounding the outer side of the test tube rack 14. A certain gap is left between the inner wall and the outer side of the test tube rack 14, and a positioning sensor is built in. On the one hand, it can form an inner protection for the test tube rack 14 and the mixing tube 13, preventing impurities inside the outer shell 1 from directly contacting the mixing tube 13, ensuring the cleanliness of the sample and reagent. On the other hand, the built-in positioning sensor can monitor the position of the positioning groove on the test tube rack 14 in real time, providing signal support for the accurate positioning of the test tube rack 14. The reserved gap can prevent friction interference between the test tube rack 14 and the inner liner 15 when the test tube rack 14 rotates, ensuring smooth rotation of the test tube rack 14. Driven sprocket 16: A circular sprocket component, fixedly installed on the upper end of the rotating shaft 5, located inside the power box 6, at the same horizontal height as the drive sprocket 17. Its outer side is connected to the drive sprocket 17 through the chain 18. It can receive the power transmitted by the drive sprocket 17 through the chain 18, and drive the rotating shaft 5 to rotate synchronously, realizing the transmission of power from the servo motor 7 to the rotating shaft 5. It is a key component of the power transmission chain. Drive sprocket 17: A circular sprocket component, fixedly mounted on the output shaft of the servo motor 7, located inside the power box 6, and connected to the driven sprocket 16 on the outside via a chain 18. It rotates synchronously with the output shaft of the servo motor 7, and transmits the power of the servo motor 7 to the driven sprocket 16 via the chain 18, thereby driving the rotating shaft 5 to rotate. It is the starting transmission component for power transmission. Chain 18: A ring-shaped transmission component located inside the power box 6, sleeved on the outside of the drive sprocket 17 and the driven sprocket 16. It transmits power by meshing with the teeth of the two sprockets, and can stably transmit the rotational power of the drive sprocket 17 to the driven sprocket 16, ensuring the continuity and stability of power transmission, so that the rotating shaft 5 can rotate or stop synchronously with the start and stop of the servo motor 7.

[0023] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. An AO / PI cell viability assay kit adapted to multiple detection platforms, comprising a shell (1), characterized in that: A sealing door (2) is rotatably connected above the outer shell (1), a sealing ring (3) is fixedly installed on the sealing door (2), and a power box (6) is fixedly installed above the outer shell (1). A servo motor (7) is fixedly installed at the bottom of the power box (6), and an active sprocket (17) is fixedly installed on the output shaft of the servo motor (7). A rotating shaft (5) is rotatably installed inside the power box (6), and a driven sprocket (16) is fixedly installed at the upper end of the rotating shaft (5). The driving sprocket (17) and the driven sprocket (16) are connected by a chain (18). The lower end of the rotating shaft (5) passes through the top of the outer shell (1) and is rotatably installed inside the outer shell (1). A bearing is provided at the point where the rotating shaft (5) passes through the outer shell (1). The outer shell (1) has an inner liner (15) fixedly installed inside. The inner liner (15) is annular and surrounds the outside of the test tube rack (14). The inner liner (15) has a positioning sensor built in it. There is a gap between the inner wall of the inner liner (15) and the outside of the test tube rack (14). The test tube rack (14) is fixedly installed on the part of the rotating shaft (5) located inside the inner liner (15). The test tube rack (14) is provided with multiple positioning slots. A mixing tube (13) is placed in the positioning slot. A sampling needle (4) is fixedly installed on the top of the outer shell (1). The lower end of the sampling needle (4) penetrates the top of the outer shell (1) and extends into the interior of the outer shell (1). The lower end of the sampling needle (4) is set to correspond to the opening of the mixing tube (13) below. A multi-chamber storage chamber (8) is fixedly installed on the top of the outer shell (1). An AO solution outlet (9), a PI solution outlet (11), and a buffer solution outlet (12) are respectively opened on the storage chamber (8). A solenoid valve (10) is fixedly installed at each of the AO solution outlet (9), the PI solution outlet (11), and the buffer solution outlet (12). A pipe is connected to the outlet end of the solenoid valve (10). The lower end of the pipe penetrates the top of the outer shell (1) and extends into the interior of the outer shell (1). The end of the pipe is set to correspond to the opening of the mixing tube (13) below.

2. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 1, characterized in that: The servo motor (7) is fitted with a shock absorber cover on its outer side. Multiple shock absorber springs are fixedly installed between the inner wall of the shock absorber cover and the outer wall of the servo motor (7). The bottom of the shock absorber cover is fixedly installed at the bottom of the power box (6).

3. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 2, characterized in that: The positioning sensor built into the inner liner (15) is an infrared positioning sensor, and the number of infrared positioning sensors is the same as the number of positioning slots on the test tube rack (14), with each infrared positioning sensor corresponding to one positioning slot.

4. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 3, characterized in that: An anti-slip silicone pad is fixedly installed on the inner wall of the positioning groove on the test tube rack (14), and the surface of the anti-slip silicone pad is provided with anti-slip texture.

5. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 4, characterized in that: Each chamber of the storage compartment (8) has a scale line on its outer side, and the storage compartment (8) is made of transparent polycarbonate material.

6. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 5, characterized in that: The solenoid valve (10) has a flexible tube made of polytetrafluoroethylene, and a guide nozzle is fixedly installed at the end of the tube, with the outlet end of the guide nozzle set at an angle of 45°.

7. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 6, characterized in that: A shock-absorbing pad is fixedly installed at the bottom inside the outer shell (1). The shock-absorbing pad is made of nitrile rubber and is positioned above the bottom of the inner liner (15).

8. The AO / PI cell viability assay kit adapted to multiple detection platforms according to claim 7, characterized in that: The sampling needle (4) is fitted with a protective sleeve on the outside. The protective sleeve is fixedly connected to the top of the outer shell (1). The protective sleeve is made of stainless steel and there is a gap between the inner wall of the protective sleeve and the outer wall of the sampling needle (4).

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