Microfluidic chip for genetic detection

Abstract

This utility model relates to the field of gene detection technology, specifically disclosing a microfluidic chip for gene detection, including a substrate for support. A liquid inlet component is provided at one edge of the substrate for adding the liquid to be detected. An installation area is formed on the inner side of the substrate, and a microcirculation component is provided on the inner side of the installation area. A heating component is provided on the outer side of the microcirculation component to form a pre-set heating environment for heating the liquid. The winding tube in the liquid inlet component of this utility model, combined with the outer screw groove of the conical stud, enables the liquid to be detected to flow at a stable rate and mix evenly when entering the chip. This feature avoids detection errors caused by unstable liquid flow rate or uneven mixing, providing a stable and uniform sample for subsequent gene detection steps, and improving the accuracy and reliability of the detection results.

Description

Technical Field

[0001] This utility model relates to the field of gene detection technology, specifically a microfluidic chip for gene detection. Background Technology

[0002] Microfluidic chips for gene detection are a new type of detection tool that integrates multiple disciplines such as microelectromechanical processing, biology, chemistry, and medicine. They play an important role in the field of gene detection by introducing biological samples containing genetic information (such as blood, saliva, etc.) into the microchannel network of the microfluidic chip through the sample inlet. Samples are processed in microreactors integrated on the chip, such as through cell lysis, nucleic acid extraction and purification, to separate genetic material from complex biological samples. Microheaters and temperature sensors on the microfluidic chip are used to perform gene amplification processes such as polymerase chain reaction (PCR), enabling large-scale replication of the target gene. Simultaneously, fluorescence detection and electrochemical detection technologies are used to monitor and analyze the amplified gene in real time.

[0003] Typically, the heater and temperature sensor on a microcontroller chip create a preheated environment to ensure the biological sample meets the usage requirements. However, heating is usually done in close contact with one side of the circulation tube, resulting in uneven heating. Furthermore, the dripping rate of the biological sample is unstable, potentially causing turbulence within the chip and affecting the normal flow and distribution of the liquid. Too slow a dripping rate may cause the liquid to dry out at the inlet, affecting the dripping effect. Therefore, this application provides a microfluidic chip for gene detection. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a microfluidic chip for gene detection, which solves the problems in existing technologies where the heater is usually only attached to one side of the circulation tube for heating, resulting in uneven heating, unstable dripping speed when adding biological samples, and turbulence inside the chip caused by excessive speed, affecting the normal flow and distribution of the liquid and thus the sample addition effect.

[0005] The present invention discloses a microfluidic chip for gene detection, comprising a substrate for support, a liquid inlet assembly disposed at one edge of the substrate for dripping the liquid to be detected, an installation area disposed on the inner side of the substrate, a microcirculation assembly disposed on the inner side of the installation area, and a heating assembly disposed on the outer side of the microcirculation assembly for forming a pre-set heating environment to heat the liquid. The liquid inlet assembly includes a fixing block, the top of which has a cavity, a conical stud is provided inside the cavity, a screw groove is provided outside the conical stud, a winding tube is wound around the outside of the screw groove, one end of the winding tube is located at the top of the conical stud, and the other end is provided with a liquid guide plate for discharging the added liquid. The microcirculation component includes a circulation tube, one end of which is adapted to the liquid inlet component, and the other end is arranged in a bend at a preset angle to increase the circulation time of the liquid. The other end of the circulation tube is connected to an amplification microfluidic tube, the outer side of which is adapted to the heating component to preheat the environment through which the liquid flows.

[0006] As a further improvement of this utility model, two slots are provided on the inner wall of the cavity near the bottom. A guide plate is provided on the inner side of the slots, and a connection port is provided on the top of the guide plate. The top of the connection port is connected to one end of the winding tube.

[0007] As a further improvement of this utility model, a liquid outlet is provided on one side of the guide plate, and the liquid outlet is connected to the circulation pipe.

[0008] As a further improvement of this utility model, a mixing microfluidic tube is provided at the bottom of the amplification microfluidic tube, a one-way valve is provided at one end of the mixing microfluidic tube, and an electrochemical biosensor is provided at one end of the one-way valve for testing different items.

[0009] As a further improvement of this utility model, the heating assembly includes a clamping plate component, which includes a base plate and a cover plate. The top of the base plate is provided with a heating cover layer on the outside of the amplification microfluidic tube, and the outside of the heating cover layer is in contact with the outside of the amplification microfluidic tube.

[0010] As a further improvement of this utility model, a sealing groove is provided at the top edge of the base plate, and a sealing protrusion is provided at the bottom of the cover plate, the sealing protrusion being adapted to the sealing groove.

[0011] As a further improvement of this utility model, one or more through holes are opened at one end of the circulation pipe and a branch pipe is installed thereon. A valve body is provided in the middle of the branch pipe for regulating the liquid transmission speed.

[0012] As a further improvement of this utility model, the top of the fixing block is provided with a liquid inlet, which is connected to one end of the winding tube. The inner side of the fixing block is provided with an inner cavity, and one or more locking blocks are provided inside the inner cavity. The locking blocks are engaged with the outer side of the conical stud.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: The spiral tube in the liquid inlet assembly of this invention, in conjunction with the outer spiral groove of the conical stud, enables the liquid to be tested to flow at a stable rate and mix evenly when entering the chip. This feature avoids detection errors caused by unstable liquid flow rate or uneven mixing, providing a stable and uniform sample for subsequent gene detection steps and improving the accuracy and reliability of the detection results. The guide plate is fixed by the slot on the inner wall of the cavity. The guide plate is connected to the winding tube through the connection port, and then connected to the circulation tube through the liquid outlet. This realizes the smooth transition of liquid from the liquid inlet component to the micro-circulation component, ensures the orderly flow of liquid in the chip, and reduces interference and disorder in the liquid flow process. The heating element's heating cover layer is tightly fitted to the outside of the amplification microfluidic tube. It is made of a highly thermally conductive material, which can uniformly and efficiently heat the liquid inside the tube, reducing heat transfer loss and enabling the liquid to quickly reach the temperature required for gene amplification. At the same time, it avoids local temperature differences, ensuring that the gene amplification reaction takes place in a uniform temperature environment, thereby improving amplification efficiency and consistency. Attached Figure Description

[0014] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a schematic diagram of the substrate, microcirculation component, liquid inlet component, heating component, mixing microfluidic tube, and electrochemical biosensor of this utility model. Figure 2 This is a schematic diagram of the combined structure of the heating component and the amplification circulation tube of this utility model; Figure 3 This is a front view structural diagram of the liquid inlet assembly of this utility model; Figure 4 This is a top view of the guide plate structure in the liquid inlet assembly of this utility model.

[0015] In the diagram: 1. Substrate; 2. Microcirculation assembly; 3. Liquid inlet assembly; 4. Heating assembly; 5. Mixing microfluidic tube; 6. Electrochemical biosensor; 7. One-way valve; 8. Amplification microfluidic tube; 21. Circulation pipe 1; 22. Branch pipe; 23. Valve body; 31. Fixing block; 32. Liquid inlet; 33. Inner cavity; 34. Locking block; 35. Conical stud; 36. Spiral wound tube; 37. Threaded groove; 38. Locking groove; 39. Guide plate; 310. Liquid outlet; 311. Connection port; 41. Sealing groove; 42. Clamping plate component; 43. Heating cover layer. Detailed Implementation

[0016] The following illustrations will reveal several embodiments of the present invention. For clarity, many physical details will be described in the following description. However, it should be understood that these physical details should not be used to limit the present invention. That is, in some embodiments of the present invention, these physical details are not essential. Furthermore, for the sake of simplicity, some conventional structures and components will be shown in a simple schematic manner in the illustrations.

[0017] Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0018] Please see Figure 1 , Figure 2 Although the heater and temperature sensor on the microcontroller chip can preheat to create a suitable environment, the heater usually only heats the side of the circulation tube 21, resulting in uneven heating.

[0019] When adding biological samples, the adding speed is unstable. If the speed is too fast, turbulence will be formed inside the chip, affecting the normal flow and distribution of the liquid; if the speed is too slow, the liquid will dry up at the inlet, affecting the sample addition effect. Based on this, this application provides a microfluidic chip for gene detection, including a substrate 1 for support. A liquid inlet component 3 is provided at one edge of the substrate 1 for adding the liquid to be detected. An installation area is opened on the inner side of the substrate 1. A microcirculation component 2 is provided on the inner side of the installation area. A heating component 4 is provided on the outer side of the microcirculation component 2 to form a pre-set heating environment for heating the liquid. The liquid inlet assembly 3 includes a fixing block 31, the top of the fixing block 31 is provided with a cavity, a conical stud 35 is provided on the inner side of the cavity, a screw groove 37 is provided on the outer side of the conical stud 35, and a winding tube 36 is wound around the outer side of the screw groove 37. One end of the winding tube 36 is located at the top of the conical stud 35, and the other end is provided with a liquid guide plate for discharging the added liquid. The microcirculation component 2 includes a circulation tube 21, one end of which is adapted to the liquid inlet component 3, and the other end is arranged in a preset angle to increase the circulation time of the liquid. The other end of the circulation tube is connected to an amplification microfluidic tube 8, the outer side of which is adapted to the heating component 4 to preheat the environment through which the liquid flows.

[0020] A fixing block 31 is disposed on one side edge of the substrate 1, serving to support and fix other components. A cavity is formed at the top of the fixing block 31, with a tapered stud 35 located inside the cavity and a threaded groove 37 formed on its outer side. The fixing block 31 is wound around the outside of the threaded groove 37, with one end of its top located at the top of the tapered stud 35 and the other end provided with a liquid guide plate. The liquid to be tested enters from the top of the winding tube 36 and flows along the winding tube 36 within the threaded groove 37. Due to the spiral structure of the winding tube 36, the liquid experiences a certain amount of resistance during flow, thereby slowing down the flow rate and preventing excessively rapid dripping. Meanwhile, the spiral structure also helps to mix the liquid more evenly and improve its stability. When the liquid flows to the other end of the spiral tube 36, it is discharged through the liquid guide plate and stably dripped into the subsequent microcirculation component 2.

[0021] The microcirculation component 2 is used to realize the circulation and treatment of liquids, specifically including: One end of the circulation tube 21 is adapted to the liquid inlet assembly 3, allowing the liquid to be tested, which is discharged from the liquid inlet assembly 3, to smoothly enter the circulation tube 21. The other end of the circulation tube 21 is arranged in a pre-set angle with a bend. This bend design increases the flow path of the liquid within the circulation tube, thereby increasing the circulation time. During the liquid circulation process, better mixing and reaction can occur, preparing the liquid for subsequent operations such as gene amplification.

[0022] The other end of the circulation tube 21 is connected to the amplification microfluidic tube 8. The outer side of the amplification microfluidic tube 8 is adapted to the heating component 4. When the liquid enters the amplification microfluidic tube 8 from the circulation tube 21, the heating component 4 will preheat the environment through which the liquid flows in the circulation tube 21. The amplification microfluidic tube 8 usually has a small diameter, which enables the liquid to form a microfluidic state in it, which is beneficial to improve the efficiency and accuracy of gene amplification.

[0023] Heating component 4 is used to heat the liquid, forming a pre-set heating environment. Heating component 4 is adapted to the outside of the amplification microfluidic tube 8 and can heat the liquid inside the amplification microfluidic tube 8. To overcome the problem of uneven heating in the prior art, heating component 4 can adopt a surround or multi-point heating method. For example, multiple heating units can be arranged around the outside of the amplification microfluidic tube 8, or multiple heating points can be set at different locations, so that heat can be transferred to the liquid more evenly, ensuring that the liquid is in a suitable and uniform temperature environment during the amplification process.

[0024] During gene detection, the biological sample to be tested is first added dropwise through the liquid inlet assembly 3. After the sample enters the cavity at the top of the fixing block 31, it flows along the winding tube 36 inside the spiral groove 37 on the outside of the conical stud 35. During the flow, the flow rate is stabilized and the sample is mixed evenly. Then, it is dripped into the circulation tube 21 through the liquid guide plate. The liquid passes through a bend at a preset angle in the circulation tube 21 to increase the circulation time and further mix and react. Next, the liquid enters the amplification microfluidic tube 8. At this time, the heating assembly 4 starts working to evenly heat the liquid in the amplification microfluidic tube 8, providing a suitable temperature environment for gene amplification. During this process, gene amplification and other reactions take place in the amplification microfluidic tube 8, ultimately realizing the detection and analysis of genes.

[0025] Please see Figure 1 , Figure 2 , Figure 3 as well as Figure 4 The inner wall of the cavity is provided with two slots 38 near the bottom. A guide plate 39 is provided on the inner side of the slots 38. A connection port 311 is provided on the top of the guide plate 39. The top of the connection port 311 is connected to one end of the winding tube 36.

[0026] The guide plate 39 has a liquid outlet 310 on one side, and the liquid outlet 310 is connected to the circulation pipe 21.

[0027] The bottom of the amplification microfluidic tube 8 is provided with a mixing microfluidic tube 5, one end of the mixing microfluidic tube 5 is provided with a one-way valve 7, and one end of the one-way valve 7 is provided with an electrochemical biosensor 6 for testing different items.

[0028] In the microfluidic chip used for gene detection, the liquid inlet assembly 3 is responsible for stably dripping the liquid to be tested. The cavity at the top of the fixing block 31 serves to contain and guide the liquid. Near the bottom of the inner wall of the cavity, there are two slots 38. The function of these two slots 38 is to fix the guide plate 39 and ensure that the guide plate 39 is in a stable position in the cavity and will not shake or shift due to factors such as liquid flow, thereby ensuring that the liquid can flow along the predetermined path.

[0029] The top of the guide plate 39 is provided with a connection port 311, which is connected to one end of the winding tube 36. When the liquid to be tested enters from the top of the winding tube 36 and flows along the winding tube 36 to the bottom, it will enter the guide plate 39 through this connection port 311. The guide plate 39 plays a role in further guiding the flow of liquid, and it can guide the liquid flowing out of the winding tube 36 in an orderly manner to a specific direction.

[0030] A liquid outlet 310 is provided on one side of the guide plate 39. This liquid outlet 310 is connected to the circulation pipe 21. The liquid guided by the guide plate 39 will enter the circulation pipe 21 through the liquid outlet 310. In this way, the guide plate 39 completes the task of smoothly transitioning the liquid from the liquid inlet component 3 to the micro-circulation component 2, so that the liquid can smoothly enter the subsequent circulation and processing process.

[0031] The amplification microfluidic tube 8 is a key component for gene amplification in the microfluidic chip. At the bottom of the amplification microfluidic tube 8 is a mixing microfluidic tube 5. After the liquid completes gene amplification and other reactions within the amplification microfluidic tube 8, it flows into the mixing microfluidic tube 5. One end of the mixing microfluidic tube 5 is equipped with a one-way valve 7. The function of the one-way valve 7 is to control the flow direction of the liquid, allowing the liquid to flow in only one specific direction and preventing backflow. This is crucial for ensuring the orderly flow of liquid within the microfluidic chip, because if backflow occurs, it may cause liquids from different stages to mix, affecting the accuracy of the detection results and potentially causing blockages within the chip.

[0032] An electrochemical biosensor 6 is installed at one end of the one-way valve 7. The electrochemical biosensor 6 can perform various tests on the liquid after amplification and mixing. It utilizes electrochemical principles to detect information such as the concentration and activity of specific substances in the liquid. In gene detection, the electrochemical biosensor 6 can detect relevant information of the amplified gene fragments. By analyzing this information, accurate detection and analysis of genes can be achieved, providing important basis for subsequent diagnosis and research.

[0033] In the entire gene detection process, the biological sample to be tested first enters the chip through the liquid inlet component 3. After the sample is stabilized and uniformly mixed in the winding tube 36, it is guided into the circulation tube 21 by the guide plate 39. In the circulation tube 21, the liquid increases the circulation time and further mixes and reacts. Then it enters the amplification microfluidic tube 8 for gene amplification. The amplified liquid flows into the mixing microfluidic tube 5 and, under the control of the one-way valve 7, finally reaches the electrochemical biosensor 6 for testing different items, thus completing the entire gene detection process. The close cooperation between the components ensures the accuracy and reliability of gene detection.

[0034] Please see Figure 1 , Figure 2 The heating assembly 4 includes a clamping plate component 42, which includes a base plate and a cover plate. The top of the base plate is provided with a heating cover layer 43 on the outside of the amplification microfluidic tube 8, and the outside of the heating cover layer 43 is attached to the outside of the amplification microfluidic tube 8.

[0035] A sealing groove 41 is provided at the top edge of the base plate, and a sealing protrusion is provided at the bottom of the cover plate, the sealing protrusion being adapted to the sealing groove 41.

[0036] One end of the circulation pipe 21 is provided with one or more through holes and a branch pipe 22 is installed thereon. A valve body 23 is provided in the middle of the branch pipe 22 for regulating the liquid transmission speed.

[0037] The top of the fixing block 31 is provided with a liquid inlet 32, which is connected to one end of the winding tube 36. The inner side of the fixing block 31 is provided with an inner cavity 33, and one or more locking blocks 34 are provided on the inner side of the inner cavity 33. The locking blocks 34 are engaged with the outer side of the tapered stud 35.

[0038] In the microfluidic chip for gene detection, the heating component 4 is used to provide a suitable and uniform temperature environment for the liquid in the amplification microfluidic tube 8 to ensure the smooth progress of the gene amplification process. The heating component 4 includes a clamping plate component 42, which consists of a base plate and a cover plate.

[0039] A heating cover layer 43 is provided on the top of the base plate outside the amplification microfluidic tube 8. The outer side of the heating cover layer 43 is closely attached to the outer side of the amplification microfluidic tube 8. The heating cover layer 43 can generate heat and transfer it to the amplification microfluidic tube 8, thereby heating the liquid inside the tube. This attached design can reduce heat loss during the heat transfer process and improve heating efficiency. Moreover, since the heating cover layer 43 surrounds the outer side of the amplification microfluidic tube 8, compared with the traditional one-sided heating method, the heat can be more evenly distributed on the amplification microfluidic tube 8, avoiding the problem of poor gene amplification effect caused by uneven heating.

[0040] The clamping component 42 of the heating assembly 4 consists of a base plate and a cover plate. A heating cover layer 43 is provided on the top outside the amplification microfluidic tube 8. The heating cover layer 43 is usually made of a material with good thermal conductivity, such as graphene or metal alloy, which can quickly and effectively convert electrical energy into heat energy and transfer the heat to the amplification microfluidic tube 8.

[0041] The heating cover layer 43 is tightly attached to the outside of the amplification microfluidic tube 8. On the one hand, it can minimize the thermal resistance during heat transfer and improve heating efficiency, so that the liquid in the amplification microfluidic tube 8 can quickly reach the required temperature. On the other hand, the tight attachment can ensure that the heat is evenly distributed on the entire circumference of the amplification microfluidic tube 8, avoiding local overheating or underheating, thereby ensuring that the gene amplification reaction is carried out in a uniform temperature environment and improving the consistency and reliability of amplification.

[0042] A sealing groove 41 is provided at the top edge of the base plate, while a sealing protrusion is provided at the bottom of the cover plate. The sealing protrusion is adapted to the sealing groove 41. When the cover plate is closed on the base plate, the sealing protrusion is embedded in the sealing groove 41, forming a good sealing effect, which can prevent heat loss and ensure the temperature stability inside the heating component 4. At the same time, it can also prevent external factors from interfering with the heating process, such as dust and moisture entering the heating area and affecting the heating effect and normal operation of the chip.

[0043] One or more through holes are provided at one end of the circulation tube 21, and a branch tube 22 is installed thereon. These through holes provide channels for liquid to enter the branch tube 22. A valve body 23 is provided in the middle of the branch tube 22. The valve body 23 can regulate the liquid transmission speed. During the gene detection process, different detection items may require different liquid flow rates. By adjusting the valve body 23, the flow rate of liquid in the branch tube 22 can be precisely controlled to meet different detection requirements. For example, for some detection items that require a long reaction time, the liquid flow rate can be appropriately slowed down to allow the liquid enough time to react in the chip; while for some detection items with high time requirements, the liquid flow rate can be increased to improve detection efficiency.

[0044] The top of the fixing block 31 is provided with a liquid inlet 32, which is connected to one end of the winding tube 36. The biological sample to be tested enters the winding tube 36 through the liquid inlet 32 ​​and begins to flow and be processed within the chip. The design of the liquid inlet 32 ​​facilitates the addition of samples, and the connection with the winding tube 36 ensures that the samples can smoothly enter the subsequent processing steps.

[0045] The fixing block 31 has an inner cavity 33 on its inner side, and one or more locking blocks 34 are provided on the inner side of the inner cavity 33. The locking blocks 34 engage with the outer side of the conical stud 35. The function of the locking blocks 34 is to fix the conical stud 35 and keep it in a stable position in the inner cavity 33 of the fixing block 31. When the liquid enters the cavity of the fixing block 31, it will flow along the winding tube 36 in the outer screw groove 37 of the conical stud 35. The stable position of the conical stud 35 can ensure the stability of the liquid flow path, thereby ensuring the stable flow rate and uniform mixing of the sample in the liquid inlet assembly 3, laying a good foundation for the subsequent gene detection steps.

[0046] During gene detection, the biological sample to be tested is first added to the chip through the inlet 32 ​​at the top of the fixing block 31. After the sample enters the winding tube 36, it is guided by the conical stud 35 to stabilize the flow rate and mix evenly. Then, it enters the circulation tube 21 through the guide plate 39. In the circulation tube 21, the liquid transfer speed is controlled by adjusting the valve body 23 in the middle of the branch tube 22 according to the detection requirements. After the liquid circulates for a longer time and undergoes further mixing and reaction in the circulation tube 21, it enters the amplification microfluidic tube 8. At this time, the heating component 4 starts to work. The heating cover layer 43 on the bottom plate is tightly attached to the outside of the amplification microfluidic tube 8, uniformly heating the liquid in the tube and providing a suitable temperature environment for gene amplification. At the same time, the cover plate and the bottom plate are sealed by the cooperation of the sealing protrusion and the sealing groove 41 to ensure the stability of the heating process. Finally, the liquid after amplification and other processing enters the subsequent detection stage, such as testing different items through the electrochemical biosensor 6, to complete the entire gene detection process.

[0047] The above description is merely an embodiment of this utility model and is not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of this utility model should be included within the scope of the claims of this utility model.

Claims

1. A microfluidic chip for gene detection, comprising a substrate (1) for support, wherein a liquid inlet assembly (3) is provided at one edge of the substrate (1) for adding liquid to be detected, an installation area is provided on the inner side of the substrate (1), a microcirculation assembly (2) is provided on the inner side of the installation area, and a heating assembly (4) is provided on the outer side of the microcirculation assembly (2) for forming a pre-set heating environment to heat the liquid; Its features are: The liquid inlet assembly (3) includes a fixing block (31), the top of the fixing block (31) is provided with a cavity, a conical stud (35) is provided inside the cavity, a screw groove (37) is provided outside the conical stud (35), a winding tube (36) is wound around the outside of the screw groove (37), one end of the winding tube (36) is located at the top of the conical stud (35), and the other end is provided with a liquid guide plate for discharging the added liquid; The microcirculation component (2) includes a circulation tube (21), one end of which is adapted to the liquid inlet component (3), and the other end is arranged in a preset angle to increase the circulation time of the liquid. The other end of the circulation tube is connected to an amplification microfluidic tube (8), the outer side of which is adapted to the heating component (4) for preheating the environment through which the liquid flows.

2. The microfluidic chip for gene detection according to claim 1, characterized in that: Two slots (38) are provided on the inner wall of the cavity near the bottom. A guide plate (39) is provided on the inner side of the slot (38). A connection port (311) is provided on the top of the guide plate (39). The top of the connection port (311) is connected to one end of the winding tube (36).

3. A microfluidic chip for gene detection according to claim 2, characterized in that: The guide plate (39) has a liquid outlet (310) on one side, and the liquid outlet (310) is connected to the circulation pipe (21).

4. A microfluidic chip for gene detection according to claim 1, characterized in that: The bottom of the amplification microfluidic tube (8) is provided with a mixing microfluidic tube (5), one end of the mixing microfluidic tube (5) is provided with a one-way valve (7), and one end of the one-way valve (7) is provided with an electrochemical biosensor (6) for testing different items.

5. The microfluidic chip for gene detection according to claim 1, wherein: The heating assembly (4) includes a clamping plate component (42), which includes a base plate and a cover plate. The top of the base plate is provided with a heating cover layer (43) on the outside of the amplification microfluidic tube (8), and the outside of the heating cover layer (43) is attached to the outside of the amplification microfluidic tube (8).

6. The microfluidic chip for genetic detection according to claim 5, wherein: A sealing groove (41) is provided at the top edge of the base plate, and a sealing protrusion is provided at the bottom of the cover plate. The sealing protrusion is adapted to the sealing groove (41).

7. The microfluidic chip for gene detection according to claim 1, wherein: One end of the circulation pipe (21) is provided with one or more through holes and a branch pipe (22) is installed thereon. A valve body (23) is provided in the middle of the branch pipe (22) for regulating the liquid transmission speed.

8. The microfluidic chip for gene detection according to claim 1, wherein: The top of the fixing block (31) is provided with a liquid inlet (32), which is connected to one end of the winding tube (36). The inner side of the fixing block (31) is provided with an inner cavity (33), and one or more locking blocks (34) are provided on the inner side of the inner cavity (33). The locking blocks (34) are engaged with the outer side of the tapered stud (35).

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