A biological culture device suitable for electromagnetic radiation experiments

Abstract

This utility model relates to a biological culture device suitable for electromagnetic radiation experiments, including a controller, a carbon dioxide regulating component, a temperature and humidity regulating component, and a sensor component. The controller is driven and connected to the carbon dioxide regulating component and the temperature and humidity regulating component respectively. The signal output terminal of the sensor component is electrically connected to the signal input terminal of the controller. It also includes a main chamber made of acrylic material. The carbon dioxide regulating component and the temperature and humidity regulating component are connected to the main chamber. The sensor component is installed inside the main chamber, and multiple culture dishes are placed inside. The advantages of this utility model are: using acrylic sheet as the material of the main chamber allows electromagnetic radiation to pass through, enabling external placement of the electromagnetic radiation device, improving frequency flexibility, and meeting the requirements of accommodating cell culture containers in the high-frequency range (>10GHz). The light transmittance of the main chamber can reach 92-93%, allowing direct observation of cell morphology through the main chamber, satisfying real-time optical observation of cell culture.

Description

Technical Field

[0001] This invention relates to the field of cell electromagnetic radiation experimental technology, and in particular to a biological culture device suitable for electromagnetic radiation experiments. Background Technology

[0002] Testing cells for electromagnetic radiation effects and analyzing their cellular response mechanisms is of great guiding significance for the development of electromagnetic diagnostic technologies and electromagnetic diagnostic and therapeutic equipment. However, cells have extremely demanding physiological environmental requirements for survival. If they are directly exposed to an electromagnetic environment for testing electromagnetic radiation effects, cell death may occur due to substandard physiological environmental parameters, making it impossible to accurately assess the true response of cells to electromagnetic radiation.

[0003] In the biomedical field, carbon dioxide incubators are core equipment for in vitro cell culture, precisely controlling temperature, humidity, and carbon dioxide concentration to simulate the physiological environment required for cell survival. However, traditional carbon dioxide incubators have inherent limitations in cell electromagnetic radiation experiments, making them difficult to directly apply for testing the electromagnetic radiation effects on cells. 1. Traditional carbon dioxide incubators generally use metal chambers. This type of material has good shielding properties for electromagnetic signals, which prevents external electromagnetic radiation from effectively penetrating the chamber and entering the internal culture space. 2. Existing electromagnetic radiation systems are typically large in size and cannot be directly integrated into traditional incubators for radiation implementation; 3. The sealed metal cavity hindered real-time optical observation of cell status during the experiment.

[0004] For example, patent application number 201110184584.9 discloses an electromagnetic radiation experimental system for cells. Its coaxial cavity uses a metal shell and uses the coaxial cavity as a carrier of electromagnetic waves. The electromagnetic field generated can reproduce the real electromagnetic radiation scene. However, due to the strong electromagnetic shielding of the metal shell, the electromagnetic waves generated by the signal source unit cannot directly penetrate the coaxial cavity. The signal source unit can only be inside the box. Moreover, the operating frequency is strongly bound to the cavity size, resulting in poor frequency flexibility. In the high-frequency range (>10GHz), the size of the coaxial cavity can no longer accommodate the cell culture container. In addition, the material is opaque, making real-time observation inconvenient. Utility Model Content

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a biological culture device suitable for electromagnetic radiation experiments. It uses acrylic sheet as the material of the main chamber, which allows electromagnetic radiation to pass through, thus realizing the external placement of the electromagnetic radiation device and improving frequency flexibility. Under the premise of meeting the requirements of the high frequency range (>10GHz), the size of the main chamber can also accommodate cell culture containers. At the same time, the light transmittance of the acrylic sheet can reach 92-93%, allowing direct observation of cell morphology through the main chamber, meeting the requirements of real-time optical observation of cell culture, and avoiding environmental fluctuations caused by opening and closing the door.

[0006] The objective of this invention is achieved through the following technical solution: A biological culture device suitable for electromagnetic radiation experiments, comprising a controller, a carbon dioxide regulating component, a temperature and humidity regulating component, and a sensor component. The controller is driven and connected to the carbon dioxide regulating component and the temperature and humidity regulating component, respectively. The signal output terminal of the sensor component is electrically connected to the signal input terminal of the controller. The device also includes a main chamber made of acrylic material. The carbon dioxide regulating component and the temperature and humidity regulating component are connected to the main chamber, and the sensor component is installed inside the main chamber. Multiple culture dishes are placed inside the main chamber. The acrylic material has a dielectric constant of 3.5 and a loss tangent of 0.02, exhibiting excellent wave transmittance performance. This ensures minimal attenuation of external electromagnetic radiation penetrating the chamber, meeting the requirements for external electromagnetic radiation devices and thus improving frequency flexibility. Simultaneously, the acrylic sheet also possesses high optical transparency, with a light transmittance of 92%-93%, approaching that of quartz glass. Cell morphology can be directly observed through the chamber wall, satisfying real-time optical observation of cell culture while avoiding environmental fluctuations caused by opening and closing the door. Acrylic materials exhibit strong chemical stability, producing no leachable substances that contaminate cells. Meeting the biocompatibility standard of GB / T16886.1 (cytotoxicity level ≤ 1), it avoids material contamination that could lead to decreased cell viability. Under long-term use in cell culture environments (37℃, high humidity, 5% carbon dioxide concentration), it will not deform, crack, or discolor. Acrylic sheets are also strong, tough, and not easily broken, with a low density of only 1.19 g / cm³, facilitating equipment movement in experimental settings (such as adjusting the distance from electromagnetic radiation sources).

[0007] Furthermore, the carbon dioxide regulating assembly includes a gas cylinder and a solenoid valve. The outlet of the gas cylinder is connected to the inlet of the solenoid valve, the outlet of the solenoid valve is connected to the interior of the main housing, and the control terminal of the solenoid valve is electrically connected to a controller. The gas cylinder provides a carbon dioxide source to the interior of the main housing, resulting in a carbon dioxide concentration of approximately 5% inside the main housing.

[0008] Furthermore, the temperature and humidity regulating component includes a water tank, a water pump, a first air pump, and a heating element. The heating element is installed inside the water tank. The water inlet of the water pump is connected to the lower part of the water tank. A heat exchange tube is installed on the inner wall of the main tank. The water outlet of the water pump is connected to the water inlet of the heat exchange tube, and the water outlet of the heat exchange tube is connected to the upper part of the water tank. The air inlet of the first air pump is connected to the upper part of the water tank, and the air outlet of the first air pump is connected to the upper part of the main tank. The control terminals of the water pump and the first air pump are electrically connected to a controller. Hot water and steam are provided by heating the water tank. The hot water circulates to the interior of the main tank to raise the internal temperature to 37°C. The steam is pumped into the main tank by the first air pump to increase the humidity inside the main tank, thus providing both heat and humidity in one water tank.

[0009] Furthermore, the sensor assembly includes a carbon dioxide sensor, a temperature sensor, and a humidity sensor. These sensors are all mounted on the inner wall of the main chamber, enabling real-time monitoring of the carbon dioxide concentration, temperature, and humidity inside the main chamber, thus ensuring a suitable cell culture environment.

[0010] Furthermore, it also includes a second air pump and a filter. The air inlet of the second air pump is connected to the interior of the main chamber, and the air outlet is connected to the inlet of the filter. The outlet of the filter is connected to the interior of the main chamber. By using the second air pump to extract the gas from the main chamber, passing it through the filter, and then circulating it back into the main chamber, the sterile environment for cell culture inside the main chamber can be further ensured.

[0011] Furthermore, the controller is an STM32 microcontroller.

[0012] Furthermore, an exhaust pipe is connected to the lower part of the main housing.

[0013] This utility model has the following advantages: 1. Acrylic sheet is used as the material of the main chamber, which allows electromagnetic radiation to pass through, enabling the electromagnetic radiation device to be externally mounted, improving frequency flexibility, and meeting the requirements of the high-frequency range (>10GHz), so that the size of the main chamber can accommodate cell culture containers.

[0014] 2. The light transmittance of the acrylic sheet can reach 92-93%, allowing direct observation of cell morphology through the main chamber, meeting the needs of real-time optical observation of cell culture, while avoiding environmental fluctuations caused by opening and closing the door.

[0015] 3. Acrylic sheets have strong chemical stability and no leaching contaminates cells. They meet the biocompatibility standard of GB / T 16886.1 with a cytotoxicity level of ≤1, which can avoid material contamination that leads to a decrease in cell activity. They will not deform, crack, or discolor during long-term use in a cell culture environment (37℃, high humidity, 5% carbon dioxide concentration).

[0016] 4. Acrylic sheets have high strength, good toughness, are not easily broken, and have a low density of only 1.19 g / cm³, which facilitates the movement of equipment in experimental settings (such as adjusting the distance from electromagnetic radiation sources). Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model; In the diagram: 1-Controller, 2-Main housing, 3-Water tank, 4-Gas cylinder, 5-Cultural dish, 6-Solenoid valve, 7-Water pump, 8-First air pump, 9-Second air pump, 10-Filter, 11-Heating element, 12-Sensor assembly, 13-Heat exchange tube, 14-Exhaust pipe. Detailed Implementation

[0018] The present invention will be further described below with reference to the accompanying drawings, but the scope of protection of the present invention is not limited to the following description.

[0019] like Figure 1As shown, a biological culture device suitable for electromagnetic radiation experiments includes a controller 1, a carbon dioxide regulating component, a temperature and humidity regulating component, and a sensor component 12. In this embodiment, the controller 1 uses an STM32 microcontroller, whose main function is to receive the carbon dioxide concentration signal, temperature signal, and humidity signal collected by the sensor component 12, and to drive the carbon dioxide regulating component and the temperature and humidity regulating component. The controller 1 is connected to the carbon dioxide regulating component and the temperature and humidity regulating component respectively. The signal output terminal of the sensor component 12 is electrically connected to the signal input terminal of the controller 1. This biological culture device also includes a main chamber 2, which is made of acrylic material. The carbon dioxide regulating component and the temperature and humidity regulating component are connected to the main chamber 2 respectively. The sensor component 12 is installed inside the main chamber 2. Multiple culture dishes 5 are placed inside the main chamber 2. An exhaust pipe 14 is connected to the lower part of the main chamber 2. The exhaust pipe 14 leads to a tail gas treatment tank through a flexible tube, which can absorb water vapor and carbon dioxide to prevent pollution of the outside air. Acrylic material has a dielectric constant of 3.5 and a loss tangent of 0.02, exhibiting excellent wave transmittance. This ensures minimal attenuation of external electromagnetic radiation penetrating the main enclosure 2, meeting the requirements for external electromagnetic radiation devices and thus improving frequency flexibility. Simultaneously, the acrylic sheet possesses high optical transparency, with a transmittance of 92%-93%, approaching that of quartz glass. This allows direct observation of cell morphology through the main enclosure 2, satisfying real-time optical observation for cell culture while avoiding environmental fluctuations caused by opening and closing the door. Acrylic material exhibits strong chemical stability, with no leaching contaminating cells. It meets the biocompatibility standard of GB / T 16886.1 (cytotoxicity level ≤ 1), preventing material contamination that could reduce cell viability. Even under long-term use in a cell culture environment (37℃, high humidity, 5% carbon dioxide concentration), it will not deform, crack, or discolor. The acrylic sheet also boasts high strength, good toughness, and is not easily broken. Its low density of only 1.19 g / cm³ facilitates equipment movement within experimental settings (such as adjusting the distance from electromagnetic radiation sources).

[0020] In this embodiment, the sensor assembly 12 includes a carbon dioxide sensor, a temperature sensor, and a humidity sensor. All three sensors are mounted on the inner wall of the main chamber, enabling real-time monitoring of the carbon dioxide concentration, temperature, and humidity inside the main chamber, thus ensuring a suitable cell culture environment.

[0021] Furthermore, the carbon dioxide regulating component includes a gas cylinder 4 and a solenoid valve 6. The outlet of the gas cylinder 4 is connected to the inlet of the solenoid valve 6, and the outlet of the solenoid valve 6 is connected to the interior of the main housing 2. The control terminal of the solenoid valve 6 is electrically connected to the controller 1. The gas cylinder provides a carbon dioxide source to the interior of the main housing, maintaining a carbon dioxide concentration of approximately 5%. Specifically, a carbon dioxide sensor collects the carbon dioxide concentration inside the main housing 2 and transmits it to the controller 1. The controller 1 controls the opening and closing of the solenoid valve 6 based on the carbon dioxide concentration, thereby enabling real-time adjustment of the carbon dioxide concentration inside the main housing 2.

[0022] Furthermore, the temperature and humidity control assembly includes a water tank 3, a water pump 7, a first air pump 8, and a heating element 11. The heating element 11 is installed inside the water tank 3, and the heating element 11 is preferably an electric heating rod with a fast temperature rise. The water inlet of the water pump 7 is connected to the lower part of the water tank 3. A heat exchange tube 13 is installed on the inner wall of the main body 2. The water outlet of the water pump 7 is connected to the water inlet of the heat exchange tube 13, and the water outlet of the heat exchange tube 13 is connected to the upper part of the water tank 3. The air inlet of the first air pump 8 is connected to the upper part of the water tank 3, and the air outlet of the first air pump 8 is connected to the upper part of the main body 2. The control terminals of the water pump 7 and the first air pump 8 are electrically connected to the controller 1, respectively. The water tank 3 provides hot water and steam to the main chamber 2 via heating element 11. The hot water circulates to the heat exchange pipe 13 inside the main chamber 2, where it exchanges heat with the "cold air" inside the main chamber 2, thereby raising the temperature inside the main chamber 2 to 37°C. Meanwhile, the steam is pumped into the main chamber 2 by the first air pump 8, increasing the humidity inside the main chamber 2. Thus, the water tank 3 provides both heat and humidity.

[0023] Furthermore, to provide a better sterile environment, this bioculture device also includes a second air pump 9 and a filter 10. The air inlet of the second air pump 9 is connected to the interior of the main chamber 2, and the air outlet is connected to the inlet of the filter 10. The outlet of the filter 10 is connected to the interior of the main chamber 2. By drawing gas out of the main chamber 2 through the second air pump 9, passing it through the filter 10, and then recirculating it back into the main chamber 2, the sterile environment for cell culture inside the main chamber 2 can be further guaranteed. Preferably, the filter 10 adopts a HEPA high-efficiency air filtration module.

[0024] The working process of this utility model is as follows: The water in the water tank 3 is preheated to maintain it at about 65°C. The sensor component periodically sends the collected carbon dioxide concentration signal, temperature signal, and humidity signal to the controller 1. The controller 1 drives the carbon dioxide regulation component and the temperature and humidity regulation component according to the signals, so that the carbon dioxide concentration, temperature, and humidity in the main chamber 2 meet the requirements of cell culture (carbon dioxide concentration controlled at 5%±0.1%, temperature controlled at 37°C±0.3°C, and humidity at 95%±2%). During the culture process, cell morphology can be observed and recorded in real time through a transparent acrylic plate.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A biological culture device suitable for electromagnetic radiation experiments, comprising a controller (1), a carbon dioxide regulating component, a temperature and humidity regulating component, and a sensor component (12), wherein the controller (1) is driven and connected to the carbon dioxide regulating component and the temperature and humidity regulating component respectively, and the signal output terminal of the sensor component (12) is electrically connected to the signal input terminal of the controller (1), characterized in that: It also includes a main chamber (2), which is made of acrylic material. The carbon dioxide regulating component and the temperature and humidity regulating component are respectively connected to the main chamber (2). The sensor component (12) is installed inside the main chamber (2). Multiple petri dishes (5) are placed inside the main chamber (2).

2. The biological culture device suitable for electromagnetic radiation experiment according to claim 1, characterized in that: The carbon dioxide regulating component includes a gas cylinder (4) and a solenoid valve (6). The outlet of the gas cylinder (4) is connected to the inlet of the solenoid valve (6). The outlet of the solenoid valve (6) is connected to the interior of the main housing (2). The control end of the solenoid valve (6) is electrically connected to the controller (1).

3. A biological culture device suitable for electromagnetic radiation experiments according to claim 2, characterized in that: The temperature and humidity control assembly includes a water tank (3), a water pump (7), a first air pump (8), and a heating element (11). The heating element (11) is installed inside the water tank (3). The water inlet of the water pump (7) is connected to the lower part of the water tank (3). A heat exchange tube (13) is installed on the inner wall of the main body (2). The water outlet of the water pump (7) is connected to the water inlet of the heat exchange tube (13). The water outlet of the heat exchange tube (13) is connected to the upper part of the water tank (3). The air inlet of the first air pump (8) is connected to the upper part of the water tank (3). The air outlet of the first air pump (8) is connected to the upper part of the main body (2). The control terminals of the water pump (7) and the first air pump (8) are electrically connected to the controller (1).

4. A biological culture device suitable for electromagnetic radiation experiments according to claim 3, characterized in that: The sensor assembly (12) includes a carbon dioxide sensor, a temperature sensor, and a humidity sensor.

5. A biological culture device suitable for electromagnetic radiation experiments according to any one of claims 1-4, characterized in that: It also includes a second air pump (9) and a filter (10). The air inlet of the second air pump (9) is connected to the interior of the main housing (2), and the air outlet is connected to the inlet of the filter (10). The outlet of the filter (10) is connected to the interior of the main housing (2).

6. The biological culture device for electromagnetic radiation experiment according to claim 1, characterized in that: The controller (1) is an STM32 microcontroller.

7. A biological culture device suitable for electromagnetic radiation experiments according to claim 1, characterized in that: The lower part of the main housing (2) is connected to an exhaust pipe (14).

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