A pretreatment device for veterinary drug residue detection

Abstract

The utility model provides a kind of pretreatment device for veterinary drug residue detection, including crushing component, liquid injection component and liquid suction component, the crushing component includes stirring rod, blade and stirring paddle are sequentially provided on stirring rod, it can be used for crushing sample and also can be used for mixing sample;The liquid injection component can be required to add hydrolyzate and extract in device on time;The liquid suction component can adjust liquid suction gun head position according to the height of supernatant, accurately complete liquid suction process, can avoid error rework, can also make full use of sample to prevent waste, make liquid suction process more accurate and efficient.

Description

Technical Field

[0001] This utility model belongs to the field of veterinary drug detection technology, specifically, it relates to a pretreatment device for veterinary drug residue detection. Background Technology

[0002] Veterinary drugs are substances used to prevent, treat, or diagnose animal diseases or to purposefully regulate animal physiological functions. Some veterinary drugs can cause some harm to humans. When people eat meat containing veterinary drugs, they are more likely to develop diseases. Therefore, meat samples need to be tested. Before testing, meat samples need to undergo several pretreatments, such as crushing, to convert the samples into a test solution that can be analyzed by instruments. This requires processing by a pretreatment device for veterinary drug residue detection.

[0003] Existing pretreatment devices for veterinary drug residue detection are typically large, complex, and expensive to perform pretreatment steps such as crushing, mixing, enzymatic hydrolysis, extraction, and purification. Furthermore, these steps usually require separate devices or different chambers within a single device, which is not only time-consuming and labor-intensive but also wastes samples. For example, to detect glucocorticoid residues in pork or pig kidneys, the sample must first be crushed, then placed in a reagent tube, hydrolyzed with ammonium acetate buffer and β-glucuronidase-arylsulfatase, mixed, and allowed to stand. Then, ethyl acetate or other extraction solutions are added for extraction, followed by further mixing, standing, and separation of the supernatant. Therefore, the entire process requires at least two container changes, the use of crushers and mixers, and manual removal of the crushed sample and addition of reagents, making it cumbersome and lacking in automation. Especially during the process of settling and separating the supernatant, manual control of the pipette is required. Attention must be paid to the depth of the pipette tip into the supernatant. If it accidentally enters the lower sediment, it will be necessary to remix, settling, and remove the supernatant, which is time-consuming, laborious, and prone to errors and rework.

[0004] Therefore, there is an urgent need to find a device that can complete the crushing, stirring, enzymatic hydrolysis, extraction, and purification pretreatment processes in a single small machine, avoiding errors caused by multiple manual operations, improving sample pretreatment efficiency, and providing a favorable guarantee for subsequent detection. Utility Model Content

[0005] To address the shortcomings of existing technologies, this utility model provides a pretreatment device for veterinary drug residue detection, comprising a crushing component, a liquid injection component, and a liquid aspiration component. The crushing component includes a stirring rod with blades and a stirring paddle arranged sequentially on it, which can be used for both crushing and mixing samples. The liquid injection component can add the required hydrolysate and extractant to the device at set times. The liquid aspiration component can adjust the position of the aspiration nozzle according to the height of the supernatant to accurately complete the liquid aspiration process, avoiding errors and rework, making full use of the sample and preventing waste, thus making the liquid aspiration process more precise and efficient.

[0006] The pretreatment device for veterinary drug residue detection provided by this utility model includes an integrated structure composed of a crushing component and a liquid aspiration component. The crushing component is used to crush and stir the sample to be tested to obtain the liquid to be tested. After the liquid to be tested is allowed to stand and separate into layers, the supernatant to be tested is aspirated by the liquid aspiration component for detection.

[0007] This pretreatment device integrates crushing, mixing, hydrolysis, extraction, stratification, and liquid aspiration into a single chamber, making it particularly suitable for detecting glucocorticoid veterinary drug residues in pork or pig kidneys. The crushing component can be used for both crushing and mixing the sample. The liquid aspiration component automatically aspirates the supernatant to be analyzed and transfers it to the analytical instrument for detection.

[0008] Furthermore, the crushing component includes a stirring rod, blades, and a stirring paddle, with the blades and stirring paddle arranged sequentially at intervals on the stirring rod.

[0009] Both the blades and the agitator are connected to the stirring rod, and the blades and agitators are arranged alternately, for example, first the blades, then the agitator below the blades, then another blade below the agitator, and finally another agitator below the blades. Because both the blades and the agitator are mounted on the stirring rod, it can be used for both cutting and crushing samples and for mixing and homogenizing samples.

[0010] Furthermore, the blades are arranged horizontally or at an angle, and the stirring paddle is arranged vertically; the lateral length of the blades is longer than that of the stirring paddle.

[0011] The blades are used to cut and crush samples. A horizontal or inclined arrangement is more conducive to rapid cutting and crushing of samples, especially a horizontal arrangement, which results in higher cutting and crushing efficiency. Because the cutting process is completed during the rotation of the stirring rod, a horizontally arranged blade can make better use of the centrifugal force generated by the rotation, thus cutting the sample efficiently with greater cutting force.

[0012] The stirring paddle is used to mix and homogenize the sample. The vertically arranged stirring paddle can come into contact with more sample during the mixing process, so that more sample can enter the rotational mixing state as soon as possible, and the mixing efficiency is higher.

[0013] The longer the lateral length of the blade, the longer the cutting edge, the greater the chance of contact with the sample, and the better the cutting and crushing effect. The stirring paddle, on the other hand, is only used for stirring and mixing, and its lateral length does not need to be very long. If it is too long, it will cause too much resistance during rotation. Therefore, the lateral length of the stirring paddle can be significantly shorter than that of the blade.

[0014] Furthermore, it also includes a control motor located above the stirring rod, used to control the rotational speed of the stirring rod, thereby controlling the stirring rod to crush or stir the sample to be tested.

[0015] In some methods, when the stirring rod is used to break up the sample, the control motor can make the stirring rod rotate faster, thus breaking up the sample at high speed; when the stirring rod is used to mix the sample, the control motor can make the stirring rod rotate slower, thus mixing the sample at low speed.

[0016] Furthermore, it also includes a liquid injection component, which includes a hydrolysate injection component and an extract injection component. The hydrolysate injection component introduces hydrolysate through a first inlet at the top of the pretreatment device, and the extract injection component introduces extract through a second inlet at the top of the pretreatment device.

[0017] Furthermore, the hydrolysate injection component is equipped with a first peristaltic pump for pumping the hydrolysate into the first inlet; the extract injection component is equipped with a second peristaltic pump for pumping the extract into the second inlet.

[0018] Furthermore, the pretreatment device is provided with a liquid outlet at the upper end, and the liquid suction component draws the supernatant to be tested from the liquid outlet through a third peristaltic pump.

[0019] Furthermore, the liquid aspiration component includes a lifting rod and an outer cylinder. The first end of the lifting rod is used to fix the liquid aspiration nozzle, and the second end is located inside the outer cylinder and can move up and down inside the outer cylinder, thereby driving the nozzle to move up and down and controlling the depth of the nozzle into the supernatant to be tested.

[0020] Since the depth of the supernatant varies after processing, the height of the pipette tip of the aspiration component needs to be adjusted according to the depth. The deeper the depth, the lower the pipette tip can be set, so that more supernatant can be aspirated. During the aspiration of supernatant, the position of the pipette tip must remain fixed and cannot move up or down to avoid accidentally aspirating the precipitate, which would directly affect subsequent detection and require re-mixing, settling, and aspiration.

[0021] The pretreatment device provided by this utility model, by setting up a liquid suction component with an adjustable nozzle height as needed, makes the liquid suction process more precise and stable, which can fully absorb the supernatant and avoid accidentally aspirating the sediment.

[0022] Furthermore, the liquid suction component also includes a positioning element, which has two states: tightened and loosened. When the positioning element is in the tightened state, the lifting rod and the outer cylinder are fixed in position. When the positioning element is in the loosened state, the lifting rod can move up and down inside the outer cylinder.

[0023] In some embodiments, the positioning element includes screws and nuts. After adjusting the position of the lifting rod, tightening the screws and nuts fixes the lifting rod in a specific position within the outer cylinder, thus ensuring the nozzle at one end of the lifting rod is at a fixed height for precise aspiration of the supernatant. After aspiration is complete, simply loosening the screws and nuts allows the lifting rod to return to its free up-and-down movement state for future adjustments and use.

[0024] Furthermore, the outer wall of the outer cylinder is provided with a first track and a second track; the first end of the lifting rod extends from the first track and can move up and down along the first track; the positioning member extends from the second track and can move up and down along the second track to select a suitable position before entering the fastening state.

[0025] This utility model has the following beneficial effects:

[0026] (1) The entire pretreatment process, including crushing, stirring, enzymatic hydrolysis, extraction, and purification, can be completed in a single small device. It is particularly suitable for detecting the residual amount of glucocorticoid veterinary drugs in pork or pig kidneys.

[0027] (2) The stirring rod is equipped with both blades and a stirring paddle, which can be used to both crush and mix samples;

[0028] (3) The required hydrolysate and extract can be added to the device on time via the liquid injection component;

[0029] (4) The liquid aspiration component can adjust the position of the aspiration nozzle according to the height of the supernatant to accurately complete the liquid aspiration process. This can avoid errors and rework, make full use of the sample and prevent waste, and make the liquid aspiration process more precise and efficient.

[0030] (5) It has a simple structure and can be industrialized. Attached Figure Description

[0031] Figure 1 A schematic diagram of the overall structure of a pretreatment device for veterinary drug residue detection;

[0032] Figure 2 A three-dimensional view of the main components of a pretreatment device for veterinary drug residue detection;

[0033] Figure 3 Cross-sectional view of the main parts of the pretreatment device for veterinary drug residue detection;

[0034] Figure 4Exploded view of the main part of the pretreatment device for veterinary drug residue detection;

[0035] Figure 5 The movement of the lifting rod of the liquid suction component (left view);

[0036] Figure 6 The movement of the lifting rod of the liquid suction component (viewed on the right). Detailed Implementation

[0037] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be noted that the embodiments described below are intended to facilitate the understanding of the present invention and do not constitute any limitation thereof.

[0038] Example 1: Pretreatment device for veterinary drug residue detection

[0039] The pretreatment device 1 for veterinary drug residue detection provided by this utility model is as follows: Figures 1-4 As shown, the device comprises an integrated structure consisting of a crushing component 2 and a liquid absorption component 3. The crushing component 2 is used to crush and stir the sample to be tested to obtain the liquid to be tested. After the liquid to be tested is allowed to stand and separate into layers, the supernatant to be tested is drawn up by the liquid absorption component 3 for detection. This pretreatment device 1 integrates all the steps of crushing, stirring and mixing, hydrolysis, extraction, separation, and liquid absorption into one chamber.

[0040] like Figure 3 As shown, the crushing component 2 can be used for both crushing and mixing samples. The liquid aspiration component 3 can automatically aspirate the supernatant to be tested to the analytical instrument for analysis. The crushing component 2 includes a stirring rod 4, blades 5, and a stirring paddle 6. The blades 5 and the stirring paddle 6 are arranged alternately on the stirring rod 4. Both the blades 5 and the stirring paddle 6 are connected to the stirring rod 4, and they are arranged alternately, for example, first the blades 5, then the stirring paddle 6 below the blades 5, then another blade 5 below the stirring paddle 6, and finally another stirring paddle 6 below the blades 5. Because both the blades 5 and the stirring paddle 6 are arranged on the stirring rod 4, it can be used for both cutting and crushing samples and mixing samples.

[0041] The blades 5 are arranged horizontally or at an angle, while the stirring paddle 6 is arranged vertically; the lateral length of the blades 5 is longer than that of the stirring paddle 6. The blades 5 are used to cut and crush samples. A horizontal or angled arrangement is more conducive to rapid cutting and crushing, especially a horizontal arrangement, which results in higher cutting and crushing efficiency. Because the cutting process is completed during the rotation of the stirring rod 4, a horizontally arranged blade 5 can make better use of the centrifugal force generated during rotation, thus cutting the sample efficiently with greater cutting force. The stirring paddle 6 is used to mix and homogenize the sample. A vertically arranged stirring paddle 6 can contact more of the sample during mixing, allowing more sample to enter a rotating and homogenized state as quickly as possible, resulting in higher mixing and homogenization efficiency. The longer the lateral length of the blades 5, the longer the cutting edge 7, and the greater the chance of contact with the sample, resulting in a higher cutting and crushing effect. The stirring paddle 6 is only used for mixing and homogenization, and its lateral length does not need to be very long; an excessively long blade would lead to excessive resistance during rotation. Therefore, the lateral length of the stirring paddle 6 can be significantly shorter than that of the blades 5.

[0042] like Figures 1-4 As shown, the pretreatment device 1 is also equipped with a control motor 8, which is located above the stirring rod 4 and is used to control the rotation speed of the stirring rod 4, thereby controlling whether the stirring rod 4 is used to crush or to stir the sample to be tested. When the stirring rod 4 is used to crush the sample, the control motor 8 can make the stirring rod 4 rotate faster, thereby crushing the sample at high speed; when the stirring rod 4 is used to stir and mix the sample, the control motor 8 can make the stirring rod 4 rotate slower, thus mixing the sample at low speed.

[0043] like Figures 3-5 As shown, the pretreatment device 1 also includes a liquid injection component 9, which comprises a hydrolysate injection component 10 and an extract injection component 11. The hydrolysate injection component 10 introduces hydrolysate through a first inlet 12 at the upper end of the pretreatment device 1, and the extract injection component 11 introduces extract through a second inlet 13 at the upper end of the pretreatment device 1. The hydrolysate injection component 10 is equipped with a first peristaltic pump 14 for pumping the hydrolysate into the first inlet 12; the extract injection component 11 is equipped with a second peristaltic pump 15 for pumping the extract into the second inlet 13. The pretreatment device 1 also has an outlet 16 at its upper end, and the aspiration component 3 aspirates the supernatant to be tested from the outlet 16 via a third peristaltic pump 17.

[0044] like Figure 4 and Figure 5As shown, the liquid aspiration component 3 includes a lifting rod 18 and an outer cylinder 19. The first end 20 of the lifting rod 18 is used to fix the nozzle 28, and the second end 21 is located inside the outer cylinder 19 and can move up and down within the outer cylinder 19, thereby moving the nozzle 28 up and down and controlling the depth to which the nozzle 28 extends into the supernatant to be tested. Since the depth of the supernatant varies after different samples are processed, the nozzle 28 of the liquid aspiration component 3 needs to be adjusted according to its depth. The deeper the depth, the lower the nozzle 28 can be set, so that more supernatant can be aspirated. During the supernatant aspiration process, the position of the nozzle 28 must remain fixed and cannot move up and down to avoid accidentally aspirating the precipitate, which would directly affect subsequent detection and require remixing, settling, and aspiration. By setting up a liquid aspiration component 3 with an adjustable nozzle 28 height as needed, the liquid aspiration process is made more accurate and stable, which can fully aspirate the supernatant and avoid accidentally aspirating the precipitate.

[0045] like Figure 4 and Figure 5 As shown, the liquid aspiration component 3 also includes a positioning element 22, which has two states: tightened and loosened. When the positioning element 22 is in the tightened state, the positions of the lifting rod 18 and the outer cylinder 19 are fixed. When the positioning element 22 is in the loosened state, the lifting rod 18 can move up and down within the outer cylinder 19. In this embodiment, the positioning element 22 can be a screw 23 and a nut 24. After adjusting the position of the lifting rod 18, tightening the screw 23 and the nut 24 will fix the lifting rod 18 in a specific position within the outer cylinder 19, thereby ensuring that the nozzle 28 at one end of the lifting rod 18 is also at a fixed height for precise aspiration of the supernatant. After aspiration is complete, simply loosening the screw 23 and the nut 24 will allow the lifting rod 18 to return to its free up-and-down movement state for the next adjustment and use. The outer wall 25 of the outer cylinder 19 is provided with a first track 26 and a second track 27; the first end 20 of the lifting rod 18 extends from the first track 26 and can move up and down along the first track 26; the positioning member 22 extends from the second track 27 and can move up and down along the second track 27 to select a suitable position and then enter the fastening state.

[0046] Example 2, Detection Method

[0047] This embodiment uses the pretreatment device 1 for veterinary drug residue detection provided in Example 1 to detect the residue levels of flumethasone and triamcinolone acetonide in pork. The specific method is as follows:

[0048] (1) Take a representative 5g sample from the pork sample and place it in the pretreatment device 1. Adjust the stirring rod 4 to the high speed setting (3000r / min) by controlling the motor 8 to quickly crush the sample.

[0049] (2) Turn on the first peristaltic pump 14 and pump 24 mL of hydrolysate into the first inlet 12 of the pretreatment device 1. The hydrolysate contains 0.02 mol / L ammonium acetate buffer solution and β-glucuronidase-arylsulfatase hydrolysis (β-glucuronidase 134600 U / mL, arylsulfatase 5200 U / mL). Mix at 1000 r / min for 1 min and incubate at 37℃ for 16 h in a constant temperature incubator.

[0050] (3) Turn on the second peristaltic pump 15 and pump the extract into the second inlet 13 of the pretreatment device 1. The extract is 20 mL of ethyl acetate. Mix at 1000 r / min for 1 min.

[0051] (4) After standing for 12 hours and separating the layers, fix the positioning component 22 according to the position of the supernatant in the lower layer of the sediment, so that the lowest end of the suction nozzle is not lower than the position of the separation, turn on the third peristaltic pump 17, and draw the supernatant to be tested from the outlet 16 for analysis and detection.

[0052] While the present invention has been disclosed above, it is not limited thereto. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.

Claims

1. A pretreatment device for veterinary drug residue detection, characterized in that, The device includes an integrated structure comprising a crushing component and a liquid absorption component. The crushing component is used to crush and stir the sample to be tested to obtain the liquid to be tested. After the liquid to be tested is allowed to stand and separate into layers, the supernatant to be tested is drawn up by the liquid absorption component for detection. The device also includes a liquid injection component, which includes a hydrolysate injection component and an extract injection component. The hydrolysate injection component introduces hydrolysate from the first liquid inlet at the top of the pretreatment device, and the extract injection component introduces extract from the second liquid inlet at the top of the pretreatment device.

2. The pretreatment device for veterinary drug residue detection as described in claim 1, characterized in that, The crushing component includes a stirring rod, blades, and a stirring paddle, with the blades and stirring paddle arranged sequentially at intervals on the stirring rod.

3. The pretreatment device for veterinary drug residue detection according to claim 2, wherein The blades are arranged horizontally or at an angle, and the stirring paddle is arranged vertically; the lateral length of the blades is longer than that of the stirring paddle.

4. The pretreatment device for veterinary drug residue detection as described in claim 3, characterized in that, It also includes a control motor, which is located above the stirring rod and is used to control the rotation speed of the stirring rod, thereby controlling the stirring rod to crush or stir the sample to be tested.

5. The pretreatment device for veterinary drug residue detection according to claim 4, wherein The hydrolysate injection component is equipped with a first peristaltic pump for pumping the hydrolysate into the first inlet; the extract injection component is equipped with a second peristaltic pump for pumping the extract into the second inlet.

6. The pretreatment device for veterinary drug residue detection according to claim 1, wherein The pretreatment device is provided with a liquid outlet at the upper end, and the liquid suction component draws the supernatant to be tested from the liquid outlet through the third peristaltic pump.

7. The pretreatment device for veterinary drug residue detection as described in claim 6, characterized in that, The liquid aspiration component includes a lifting rod and an outer cylinder. The first end of the lifting rod is fixed to the nozzle, and the second end is located inside the outer cylinder. It can move up and down inside the outer cylinder, thereby moving the nozzle up and down and controlling the depth to which the nozzle is inserted into the supernatant to be tested.

8. The pretreatment device for veterinary drug residue detection as described in claim 7, characterized in that, The liquid suction component also includes a positioning element, which has two states: tightened and loosened. When the positioning element is in the tightened state, the lifting rod and the outer cylinder are fixed in position. When the positioning element is in the loosened state, the lifting rod can move up and down inside the outer cylinder.

9. The pretreatment device for veterinary drug residue detection according to claim 8, wherein The outer wall of the outer cylinder is provided with a first track and a second track; the first end of the lifting rod extends from the first track and can move up and down along the first track; the positioning member extends from the second track and can move up and down along the second track to select a suitable position and then enter the fastening state.

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