Section aid for experimental animal joints

Abstract

The utility model provides a kind of for experimental animal joint's section auxiliary device, including clamping unit, connecting assembly and adjusting assembly, the quantity of clamping unit is at least two, at least two clamping units are stacked settings, and it is detachably fixed connection by connecting assembly, for from two sides to adhere to the clamping joint;At least two clamping units include juxtaposition setting first clamping piece and second clamping piece, first clamping piece is connected with second clamping piece by adjusting assembly, adjusting assembly is configured as: the adjusting state and locking state of clamping unit are realized, when adjusting state, first clamping piece is connected with second clamping piece by adjusting assembly, and can rotate relative to second clamping piece;When locking state, first clamping piece is fixed relative to second clamping piece by adjusting assembly. The fixing of different sizes of joints can be adapted by connecting assembly, different parts of joint can be adapted by adjusting assembly, ensure section consistency, and, improve device versatility.

Description

Technical Field

[0001] This utility model relates to the field of biological slicer technology, and in particular to a slicing auxiliary device for experimental animal joints. Background Technology

[0002] To study the pathogenesis of joint diseases, histopathological analysis of joints from laboratory animals such as rats, rabbits, dogs, and monkeys is commonly performed to explore the development mechanisms of joint diseases and evaluate drug efficacy and side effects. Before histopathological analysis, joint sections need to be prepared to achieve accurate pathological diagnosis and quantitative analysis. The knee joint, as a weight-bearing joint and a common site for joint diseases, is a key target organ in pharmacodynamic and toxicity studies.

[0003] Before sectioning, the joint is soaked in fixative to maintain the morphology and structure of the tissue. Due to the tight structure of the knee joint, the fixative is difficult to fully penetrate all parts, resulting in uneven fixation and affecting the quality of subsequent sections. In addition, during the sectioning process, because the knee joint is relatively hard, it is difficult to ensure the consistency of the cut surface (i.e., the section cross-section) of the joint tissue during the sample trimming process, resulting in problems such as skewed cut surfaces or inconsistent cut surface positions. Utility Model Content

[0004] The technical problem to be solved by this utility model is to overcome the shortcomings of the prior art in ensuring the consistency of the cut surface, and the existence of cut surface skew or inconsistent cut surface position, and to provide a slicing auxiliary device for experimental animal joints.

[0005] The present invention solves the above-mentioned technical problems through the following technical solution:

[0006] A slicing aid for laboratory animal joints includes a clamping unit, a connecting assembly, and an adjusting assembly. The clamping units are at least two in number, stacked and detachably connected via the connecting assembly, for clamping the joint from both sides. Each clamping unit includes a first clamping member and a second clamping member arranged side-by-side. The first clamping member is connected to the second clamping member via the adjusting assembly. The adjusting assembly is configured to achieve an adjusting state and a locking state for the clamping units. In the adjusting state, the first clamping member is connected to the second clamping member via the adjusting assembly and can rotate relative to the second clamping member. In the locking state, the first clamping member is fixed relative to the second clamping member via the adjusting assembly.

[0007] In this solution, by adjusting the spacing between at least two clamping units through the connecting components, it can accommodate the fixing of joints of different sizes, improving the versatility of the device. When the clamping units are in the adjustment state, the angle between the first and second clamping members can be adjusted through the adjustment components to accommodate different parts of the joint; when the clamping units are in the locking state, the first and second clamping members are locked through the adjustment components, limiting the angle between the first and second clamping members, improving clamping stability and cross-sectional consistency, and also exhibiting good repeatability.

[0008] Optionally, the adjustment assembly includes an adjustment post. The sides of the first clamping member and the second clamping member are respectively provided with corresponding first connecting ears and second connecting ears. The adjustment post passes through the first connecting ears and the second connecting ears, and the first connecting ears and the second connecting ears are rotatable relative to the adjustment post. The adjustment assembly also includes an adjustment nut. The first connecting ears and the second connecting ears are respectively provided with a first connecting hole and a second connecting hole for the adjustment post to pass through. The adjustment post passes through the first connecting hole and the second connecting hole in sequence and is fixedly connected to the adjustment nut, thereby limiting the angle between the first clamping member and the second clamping member.

[0009] In this design, the adjustment component is located at the edge of the first clamping member and avoids the joint, and has a compact structure that facilitates adjustment and fastening.

[0010] Optionally, the clamping surface of the first clamping member is recessed inward in the area near the first connecting ear to form a first rotating groove, and the clamping surface of the second clamping member is recessed inward in the area corresponding to the first rotating groove to form a second rotating groove, and the portions of the first clamping member and the second clamping member that are connected to each other are respectively embedded in the second rotating groove and the first rotating groove.

[0011] In this solution, the interconnected parts of the first clamping member and the second clamping member are respectively embedded in the second rotating groove and the first rotating groove to form a mating structure, which improves the stability of the clamping unit, reduces shaking during the slicing process, and improves the consistency of the cut surface.

[0012] Optionally, at least two of the clamping units are spaced apart to form a clearance groove for avoiding the cutter. Optionally, at least one of the clamping units has a clearance groove on its clamping surface for avoiding the cutter.

[0013] In this design, the clearance groove is used for the cutter to cut the joint, ensuring the integrity of the cut surface.

[0014] Optionally, at least one of the clamping units has a recessed groove on its clamping surface to accommodate the contour surface of the joint.

[0015] In this solution, the clamping surface of the clamping unit is made to fit the outer contour of the knee joint more closely by means of the receiving groove, preventing the knee joint from sliding relative to the clamping unit, thereby further improving the stability and fit of the clamping, and improving the consistency and repeatability of the cross-section.

[0016] Optionally, at least one of the clamping units has an anti-slip pad on its clamping surface.

[0017] In this design, the anti-slip pad is used to increase the friction between the knee joint and the clamping unit, preventing the knee joint from sliding relative to the clamping unit during the slicing process, thereby improving the stability and positioning accuracy of the clamping.

[0018] Optionally, the connecting assembly includes a fixing bolt and a fixing nut. The fixing bolt passes through the edges of at least two of the clamping units and is fixedly connected to the fixing nut, thereby defining the relative position between the at least two clamping units. Optionally, the clamping unit has a fixing hole for the fixing bolt to pass through, and one of the fixing bolt and the fixing nut is pre-fixed to the area corresponding to the fixing hole of the clamping unit.

[0019] In this solution, at least two clamping units are fixedly connected by fixing bolts and fixing nuts, which improves the reliability of the connection and facilitates installation and disassembly.

[0020] The positive and progressive effects of this utility model are as follows:

[0021] By adjusting the spacing between at least two clamping units using the connecting components, the device can accommodate the fixing of joints of different sizes, improving its versatility. When the clamping units are in the adjustment state, the angle between the first and second clamping members can be adjusted using the adjustment components to accommodate different parts of the joint. When the clamping units are in the locking state, the first and second clamping members are locked using the adjustment components, limiting the angle between them, improving clamping stability and cross-sectional consistency, and offering good repeatability. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of a slicing aid device for experimental animal joints according to an embodiment of the present invention.

[0023] Figure 2 This is a schematic diagram showing the usage state of a slicing aid device for experimental animal joints according to an embodiment of the present invention.

[0024] Figure 3 This is a schematic diagram of the slicing aid device for experimental animal joints according to an embodiment of the present invention, from another perspective.

[0025] Explanation of reference numerals in the attached figures:

[0026] Clamping unit 1

[0027] First clamping component 11

[0028] Second clamping component 12

[0029] First connecting ear 111

[0030] First rotating groove 112

[0031] Second connecting ear 121

[0032] Second rotating groove 122

[0033] 13 clearance slot

[0034] Reception tank 14

[0035] Connection component 2

[0036] Fixing bolt 21

[0037] Fixing nut 22

[0038] Adjustment component 3

[0039] Adjusting column 31

[0040] Adjusting nut 32

[0041] Knee joint 4

[0042] Femur 41

[0043] Tibia 42 Detailed Implementation

[0044] The present invention will be further illustrated below by way of embodiments, but these embodiments are not intended to limit the scope of the present invention. Based on the description of the present invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the present invention.

[0045] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connection," "setting," "installation," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can mean that two components are internally connected. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0046] The terms “upper,” “lower,” “left,” “right,” “front,” “back,” “center,” “top,” “bottom,” “inner,” “outer,” “vertical,” “horizontal,” “axial,” “radial,” and “circumferential” indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of description and simplification, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0047] The terms “first”, “second”, etc., are used merely to distinguish elements with similar properties, not to indicate or imply relative importance or a specific order.

[0048] The terms “including,” “comprising,” or any other variations thereof are intended to cover non-exclusive inclusion, which includes not only the elements listed but also other elements not expressly listed.

[0049] This utility model provides a slicing auxiliary device for experimental animal joints, including a clamping unit 1, a connecting component 2, and an adjusting component 3. The number of clamping units 1 is at least two, and the at least two clamping units 1 are stacked and detachably fixedly connected by the connecting component 2 to clamp the joint from opposite sides. The distance between the at least two clamping units 1 can be adjusted by the connecting component 2 to adapt to the fixation of joints of different sizes, thereby improving the versatility of the device.

[0050] Furthermore, at least two clamping units 1 each include a first clamping member 11 and a second clamping member 12 arranged side by side. The first clamping member 11 is connected to the second clamping member 12 via an adjusting component 3. The adjusting component 3 is configured to realize an adjusting state and a locking state of the clamping unit 1. In the adjusting state, the first clamping member 11 is connected to the second clamping member 12 via the adjusting component 3 and can rotate relative to the second clamping member 12. In the locking state, the first clamping member 11 is fixed relative to the second clamping member 12 via the adjusting component 3. The angle between the first clamping member 11 and the second clamping member 12 can be adjusted by the adjusting component 3, which can adapt to different parts of the joint. The first clamping member 11 and the second clamping member 12 can be locked by the adjusting component 3, which limits the angle between the first clamping member 11 and the second clamping member 12, thereby improving clamping stability and cross-sectional consistency.

[0051] See Figure 1-3In some embodiments, two clamping units 1 are used, stacked together and detachably connected by a connecting component 2, for clamping the knee joint 4 of experimental animals such as rabbits, dogs, and monkeys. Specifically, the first clamping member 11 of the two clamping units 1 clamps the femur 41 of the knee joint 4, and the second clamping member 12 clamps the tibia 42 of the knee joint 4. The angle between the first clamping member 11 and the second clamping member 12 can be adjusted by the adjusting component 3 to accommodate the angle between the femur 41 and tibia 42 of the knee joint 4 of different experimental animals, resulting in a more stable clamping of the knee joint 4, improving the fixation and positioning effect, ensuring that the knee joint 4 can be sliced ​​along the specified anatomical location, improving the consistency of the slice, and facilitating installation and disassembly with good repeatability.

[0052] In one embodiment, at least one clamping unit 1 has an anti-slip pad (not shown) on its inner surface. The anti-slip pad is used to increase the friction between the knee joint 4 and the clamping unit 1, and to prevent the knee joint 4 from sliding relative to the clamping unit 1 during the slicing process, thereby improving the stability and positioning accuracy of the clamping.

[0053] Furthermore, the two clamping units 1 have a gap to form a clearance groove 13 for avoiding the cutter, or at least one of the two clamping units 1 has a clearance groove 13 for avoiding the cutter, and the extension length of the clearance groove 13 exceeds the extension length of the joint to ensure the integrity of the cut surface.

[0054] See Figure 1 The adjustment assembly 3 includes an adjustment post 31. The sides of the first clamping member 11 and the second clamping member 12 are respectively provided with corresponding first connecting ears 111 and second connecting ears 121. The adjustment post 31 passes through the first connecting ears 111 and the second connecting ears 121. The first connecting ears 111 and the second connecting ears 121 are rotatable relative to the adjustment post 31, thereby adjusting the angle between the first clamping member 11 and the second clamping member 12. In one embodiment, the first clamping member 11 protrudes from its side to form the first connecting ear 111, and the second clamping member 12 protrudes from its side to form the second connecting ear 121 corresponding to the first connecting ear 111, thus allowing the adjustment assembly 3 to avoid joints and resulting in a compact structure.

[0055] In one embodiment, the first clamping member 11 has a first rotating groove 112 in the region near the first connecting ear 111, and the second clamping member 12 has a second rotating groove 122 in the region near the second connecting ear 121, and the first rotating groove 112 and the second rotating groove 122 correspond to each other. Specifically, the clamping surface of the first clamping member 11 (i.e., the surface in contact with the joint) near the first connecting ear 111 is recessed inward to form the first rotating groove 112, and the clamping surface of the second clamping member 12 corresponding to the first rotating groove 112 is recessed inward to form the second rotating groove 122.

[0056] The depth of the first rotating groove 112 is adapted to the thickness of the portion of the second clamping member 12 embedded in the first rotating groove 112. Correspondingly, the depth of the second rotating groove 122 is adapted to the thickness of the portion of the first clamping member 11 embedded in the second rotating groove 122. The interlocking portion of the first clamping member 11 and the second clamping member 12 forms a fitted structure, improving the stability of the clamping unit 1, reducing wobbling during slicing, and improving the consistency of the cut surface. Furthermore, when the clamping unit 1 is in the locked state, the aforementioned fitted structure ensures that the clamping surfaces of the first clamping member 11 and the second clamping member 12 are on the same horizontal plane, thereby achieving more stable and uniform clamping of the joint.

[0057] In one embodiment, the clamping surface of the clamping unit 1 is provided with a receiving groove 14 for adapting to the local shape of the knee joint 4, so that the clamping surface of the clamping unit 1 fits the outer contour of the knee joint 4 more closely, preventing the knee joint 4 from sliding relative to the clamping unit 1, further improving the stability and fit of the clamping, and improving the consistency and repeatability of the cross-section.

[0058] In one embodiment, the adjusting assembly 3 further includes an adjusting nut 32. In this embodiment, the adjusting post 31 is a bolt. The first connecting lug 111 and the second connecting lug 121 are respectively provided with a first connecting hole and a second connecting hole for the bolt to pass through. The bolt passes through the first connecting hole and the second connecting hole in sequence and is fixedly connected to the adjusting nut 32. The first clamping member 11 and the second clamping member 12 are fixedly clamped between the head of the bolt and the adjusting nut 32 to limit the angle between the first clamping member 11 and the second clamping member 12.

[0059] In one embodiment, the connecting component 2 includes a fixing bolt 21 and a fixing nut 22. The edges of the two clamping units 1 are respectively provided with corresponding fixing holes. The fixing bolt 21 passes through the fixing holes of both in sequence and is threadedly connected to the fixing nut 22 to limit the relative position between the two clamping units 1, improve the connection reliability, and facilitate installation and disassembly.

[0060] It should be noted that this utility model does not limit the connection method between the connecting component 2 and the clamping unit 1. In another embodiment, the fixing nut 22 is pre-fixed to the area of ​​the corresponding fixing hole of any clamping unit 1 by welding, riveting, or other methods. The fixing bolt 21 passes through the fixing holes of the two clamping units 1 in sequence and is threadedly connected to the fixing nut 22, which facilitates installation and disassembly. In yet another embodiment, one of the two clamping units 1 is fixedly connected to the fixing bolt 21 by welding, riveting, or other methods, and the other has a fixing hole corresponding to the fixing bolt 21. The fixing bolt 21 passes through the fixing hole and is threadedly connected to the fixing nut 22. Optionally, the circumferential outer surface of the fixing nut 22 is provided with a knob (not shown in the figure), for example, a wing nut as in the prior art, which facilitates manual tightening or disassembly, improving the convenience of operation and installation efficiency.

[0061] The present invention discloses a method for preparing knee joint sections using an auxiliary tool for joint slicing in laboratory animals, comprising the following steps:

[0062] S1. Material Acquisition: Obtain a complete knee joint 4, preserving a certain length of femur 41 and tibia 42;

[0063] S2. Tissue fixation: The obtained knee joint 4 was placed in neutral formalin fixation solution for fixation, rinsed with running water for 10 minutes, and a large amount of skin and muscle tissue covering the knee joint 4 was removed and trimmed to expose the complete knee joint 4.

[0064] S3. Decalcification: The knee joint 4, from which excess tissue has been removed, is placed in a decalcification solution for 72 hours;

[0065] S4. Refinement: Adjust the angle between the first clamping member 11 and the second clamping member 12 to match the angle between the femur 41 and tibia 42 of the knee joint 4, place the decalcified knee joint 4 between the two clamping units 1, and clamp the knee joint 4 through the two clamping units 1; use a cutter to cut the knee joint 4 into two halves along the clearance groove 13 of the slicing auxiliary device.

[0066] S5. Dehydration: Remove the two halves of the knee joint 4 from the slicing aid after cutting and place them in the embedding cassette. Place ethanol solutions of varying concentrations into the embedding cassette for dehydration treatment to remove water from the knee joint 4.

[0067] S6. Transparency treatment: After dehydration, place knee joint 4 in a transparent agent to make knee joint 4 transparent, which facilitates the subsequent penetration of paraffin.

[0068] S7. Wax impregnation and embedding: The transparent knee joint 4 is immersed in molten paraffin wax for wax impregnation treatment, so that the paraffin wax is fully immersed in the interior of the knee joint 4; then, the wax-impregnated knee joint 4 is placed in an embedding mold, molten paraffin wax is poured in for embedding, and after the paraffin wax cools and solidifies, an embedding block is formed.

[0069] S8. Sectioning: Mount the paraffin-embedded block on a horizontal microtome. First, perform a coarse cut with a thickness of 10 micrometers, and then section it with a thickness of 3 micrometers.

[0070] While specific embodiments of this utility model have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of this utility model is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of this utility model, but all such changes and modifications fall within the scope of protection of this utility model.

Claims

1. A section assisting device for joints of experimental animals, characterized by, It includes a clamping unit, a connecting component, and an adjusting component. The number of clamping units is at least two, and the at least two clamping units are stacked and detachably fixedly connected by the connecting component, for clamping the joint from both sides. At least two of the clamping units each include a first clamping member and a second clamping member arranged side by side. The first clamping member is connected to the second clamping member through the adjusting component. The adjusting component is configured to realize an adjusting state and a locking state of the clamping unit. In the adjusting state, the first clamping member is connected to the second clamping member through the adjusting component and can rotate relative to the second clamping member. In the locking state, the first clamping member is fixed relative to the second clamping member through the adjusting component.

2. The section assisting device for joints of experimental animals according to claim 1, wherein The adjustment assembly includes an adjustment post. The sides of the first clamping member and the second clamping member are respectively provided with corresponding first connecting ears and second connecting ears. The adjustment post passes through the first connecting ears and the second connecting ears, and the first connecting ears and the second connecting ears are rotatable relative to the adjustment post.

3. The section assisting device for joints of experimental animals according to claim 2, wherein The adjustment assembly further includes an adjustment nut. The first connecting lug and the second connecting lug are respectively provided with a first connecting hole and a second connecting hole for the adjustment post to pass through. The adjustment post passes through the first connecting hole and the second connecting hole in sequence and is fixedly connected to the adjustment nut to limit the angle between the first clamping member and the second clamping member.

4. The section assisting device for joints of experimental animals according to claim 2 or 3, characterized in that, The clamping surface of the first clamping member is recessed inward in the area near the first connecting ear to form a first rotating groove. The clamping surface of the second clamping member is recessed inward in the area corresponding to the first rotating groove to form a second rotating groove. The parts of the first clamping member and the second clamping member that are connected to each other are respectively embedded in the second rotating groove and the first rotating groove.

5. The section assisting device for joints of experimental animals as claimed in claim 1, wherein At least two of the clamping units are spaced apart to form a clearance groove for avoiding the cutter.

6. The section assisting device for joints of experimental animals as claimed in claim 1, wherein At least one of the clamping units has a clearance groove on its clamping surface for avoiding the cutter.

7. The section assisting device for joints of experimental animals according to claim 5 or 6, wherein At least one of the clamping units has a recessed groove formed from its clamping surface to accommodate the contour surface of the joint.

8. The section assisting device for joints of experimental animals as claimed in claim 1, wherein At least one of the clamping units has an anti-slip pad on its clamping surface.

9. The section assisting device for joints of experimental animals as claimed in claim 2, wherein The connecting assembly includes a fixing bolt and a fixing nut. The fixing bolt passes through the edges of at least two of the clamping units and is fixedly connected to the fixing nut to define the relative position between at least two of the clamping units.

10. The sectioning aid for joints of experimental animals as claimed in claim 9, wherein The clamping unit has a fixing hole for a fixing bolt to pass through, and one of the fixing bolt and the fixing nut is pre-fixed to the area of ​​the clamping unit corresponding to the fixing hole.

Images