A device for detecting nitrogen content of soil microorganisms

Abstract

The utility model discloses a kind of soil microbial biological nitrogen content detection devices, including rack, the rack bottom end is provided with moving wheel, the chamber is provided in the rack inside, a kind of soil microbial biological nitrogen content detection device, by polytetrafluoroethylene filter membrane and granular activated carbon, double purification to extract liquid, granular activated carbon can effectively adsorb chloroform residue in extract liquid, organic impurities, polytetrafluoroethylene filter membrane can filter soil residue, microbial debris and other suspended particles, completely remove interfering substance that can affect detection result, ensure that the purity of extract liquid into detector meets the standard, after purification, extract liquid is directly conveyed to detector by sealed pipeline, without manual transfer extract liquid, avoid liquid loss in transfer process, container pollution or foreign impurities mix, realize whole-process contactless detection, avoid artificial loss and pollution, further improve the accuracy of detection result.

Description

Technical Field

[0001] This utility model relates to the field of soil microbial technology, specifically a soil microbial biological nitrogen content detection device. Background Technology

[0002] In the fields of agricultural production, ecological environment monitoring, and soil science research, soil microbial biological nitrogen content is a key indicator for assessing soil fertility, microbial activity, and ecosystem health. The accuracy of its detection results, the efficiency of the detection process, and the ease of operation directly affect the reliability of subsequent production decisions and scientific research conclusions. In existing detection technologies, the purity of the extract is difficult to guarantee, and there are too many manual operation steps, which makes the test results prone to deviation and cannot accurately reflect the true level of soil microbial nitrogen content. On the one hand, after soil samples are fumigated and extracted, chloroform and organic impurities are easily left in the extract, and soil residues, microbial remains and other suspended particles may also be mixed in. On the other hand, after purification, the extract needs to be manually transferred to the detection instrument. During the transfer process, liquid loss, container contamination or external impurities may occur. Moreover, the digestion, distillation and titration steps of the detection instrument need to be manually operated step by step. The results depend on manual reading and calculation, which is prone to random errors due to human mistakes. This can cause the interfering substances in the extract to affect the response accuracy of the detection instrument, and the manual operation can amplify the deviation, thus reducing the reliability of the test results.

[0003] Therefore, this utility model provides a soil microbial biological nitrogen content detection device to solve the above problems. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a soil microbial biological nitrogen content detection device, which solves the aforementioned problems.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a soil microbial biological nitrogen content detection device, including a frame, with casters at the bottom of the frame, a chamber inside the frame, two partitions fixedly connected inside the frame, the internal chamber of the frame being divided into a first chamber, a second chamber and a third chamber by the partitions, a processing mechanism inside the third chamber, and a detector on the frame; The processing mechanism includes a processing box and an upper part of a processing tank. The processing box is a closed shell within the third chamber, providing a stable working environment for the processing tank and fixing a connecting frame and a distributor. The connecting frame is fixedly connected inside the processing box. The upper part of the processing tank is the fixed part of the processing tank and can be bolted to the tank cover for sealing. The outer barrier ring enhances the sealing between the tank cover and the tank body, and the outer limit rod prevents the lower part of the processing tank from shifting when rotating. The lower part of the processing tank is rotatably connected to the lower side of the upper part of the processing tank. The lower part of the processing tank is the rotating part of the processing tank and can hold soil samples. Rotation is achieved through gear transmission. With the help of a stirring rod, the soil can fully contact chloroform vapor, solving the problem of uneven fumigation. A connector is fixedly connected to the outer side of the lower part of the processing tank. The connector can engage with the first gear to transmit gear power to drive the lower part of the processing tank to rotate. A barrier ring is fixedly connected to the outer side of the upper part of the processing tank, and a limit rod is fixedly connected to the outer side of the upper part of the processing tank. Preferably, a processing tank cover is bolted to the upper end of the processing tank. The processing tank cover covers the upper end of the processing tank and seals the tank opening with bolts. The integrated gas valve, water pipe, and gas pipe interface on the treatment tank cover are the core channels for reagent injection, gas discharge, and extract extraction. A second handle is fixedly connected to the processing tank cover for easy opening and closing of the tank cover and for easy sample loading and unloading. A second connecting water pipe and a gas connecting pipe are provided on the processing tank cover. A first connecting water pipe is connected to the processing tank cover. A third distributor is fixedly connected to the inside of the processing tank. A gas injection port is provided on the processing tank cover. A flexible hose is connected to the gas injection port. The end of the flexible hose away from the gas injection port is connected to a third vacuum pump. The third vacuum pump is connected to an external waste gas treatment device through a pipe. A vacuum valve is provided on the flexible hose. Preferably, a first gear is rotatably connected to the bottom of the processing tank, a drive motor is fixedly connected to the connecting frame, a second gear is fixedly connected to the output end of the drive motor via a shaft, the outer side of the second gear meshes with the first gear, the first gear is fitted with a connecting piece, a first distributor is fixedly connected to the connecting frame, and a chloroform concentration sensor is provided inside the lower end of the processing tank. Preferably, a stirring rod is fixedly connected to the lower side of the treatment tank cover. When the stirring rod rotates at the lower end of the treatment tank, it can form relative motion with the soil, turning the soil and ensuring that chloroform vapor and extractant are in full contact with the soil. A reinforcing ring is fixedly connected to the stirring rod, which can enhance the structural strength of the stirring rod and prevent the rod from deforming due to soil resistance during stirring. A water-absorbing steel pipe is fixedly connected to the treatment tank cover. The drain hole in the wall of the water-absorbing steel pipe is used to draw in the extractant and serves as a channel carrier for transporting the extractant from the treatment tank to the detector. The water-absorbing steel pipe has a drain hole. Preferably, a protective component is threadedly connected to the lower side of the treatment tank cover. The protective component can fix the filter membrane and activated carbon to prevent the filter assembly from shifting. The protective component is set on the outside of the water absorption steel pipe, and the inner wall of the protective component is attached to the outer wall of the water absorption steel pipe, covering the drainage hole at the lower end of the water absorption steel pipe. A polytetrafluoroethylene (PTFE) filter membrane is provided inside the protective component. The PTFE filter membrane can filter soil particles in the extract to prevent particles from clogging subsequent pipelines or damaging the detector. Granular activated carbon is fixedly connected inside the PTFE filter membrane. The granular activated carbon can adsorb residual chloroform in the extract to prevent chloroform from interfering with the nitrogen concentration detection results. The first connecting water pipe is internally connected to the water absorption steel pipe. Preferably, the first chamber is provided with a first container, a chloroform storage tank and a second container. The second chamber is fixedly connected with a first vacuum pump, a second vacuum pump and a first air pump. The first container is connected to the first vacuum pump through a pipe. The end of the first vacuum pump away from the first container is connected to a third distributor through a pipe. The third distributor is connected to a second connecting water pipe. Preferably, the chloroform storage tank is connected to a first air pump via a gas pipe, the first air pump is connected to a second distributor via a gas pipe, the second distributor is connected to a gas connection pipe, the end of the first connecting water pipe away from the treatment tank cover is connected to a second vacuum pump, the second vacuum pump is connected to a detector via a pipe, and the detector is connected to a second container. Preferably, a second distributor is fixedly connected to the connecting frame, the second distributor is connected to the first air pump through an air pipe, a first handle is fixedly connected to the rear side of the frame, a heating jacket is provided on the connecting frame, the detector is a Kjeldahl nitrogen analyzer, and all connections are sealed.

[0006] Beneficial effects This invention provides a device for detecting nitrogen content in soil microorganisms. Compared with the prior art, it has the following advantages: (1) A soil microbial biological nitrogen content detection device, which uses polytetrafluoroethylene filter membrane and granular activated carbon to purify the extract. The granular activated carbon can effectively adsorb chloroform residue and organic impurities in the extract, while the polytetrafluoroethylene filter membrane can filter suspended particles such as soil residue and microbial remains, thoroughly removing interfering substances that may affect the detection results, ensuring that the purity of the extract entering the detector meets the standard. The purified extract is directly transported to the detector through a closed pipeline, eliminating the need for manual transfer of the extract, avoiding liquid loss, container contamination or external impurities during the transfer process, realizing contactless detection throughout the process, avoiding human loss and contamination, and further improving the accuracy of the detection results.

[0007] (2) A soil microbial biological nitrogen content detection device, which divides the first chamber, second chamber and third chamber by partition, integrates the reagents, power components and treatment tank required for detection into the same device, thereby eliminating the cross-device transfer link, greatly shortening the operation path, reducing the physical consumption of manual handling and the cumbersome operation.

[0008] (3) A soil microbial biological nitrogen content detection device, by setting up multiple processing tanks to work simultaneously, can put the sample to be tested and the blank control group sample into independent processing tanks respectively, and process multiple samples to be tested at the same time. It can complete the detection of two or more samples in the same time, greatly shorten the total cycle of batch detection, increase the detection volume per unit time, and the entire detection process is automated by means of intelligent linkage of valves, pumps and sensors, completely eliminating the time gap of waiting for a certain step to end, further improving detection efficiency, ensuring the smoothness of continuous detection of multiple batches of samples, and eliminating the gap of waiting for manual processing. Attached Figure Description

[0009] Figure 1 This is a side view of the overall device structure of this utility model; Figure 2 This is a structural diagram of the overall device of this utility model; Figure 3 This is a front view of the overall device structure of this utility model; Figure 4 This is a side view of the processing box structure of this utility model; Figure 5 This is a side view of the processing tank structure of this utility model; Figure 6 This is a side view of the processing mechanism of this utility model; Figure 7 This is a side view of the internal unfolded structure of the processing mechanism of this utility model; Figure 8 This is the utility model Figure 4 Enlarged view of partial structure A; Figure 9 This is the utility model Figure 7 Enlarged view of partial B structure; Figure 10 This is the utility model Figure 7 Enlarged view of a local C-structure.

[0010] In the diagram: 1. Frame; 2. Casters; 3. First handle; 4. Partition; 5. First container; 6. Chloroform storage tank; 7. Second container; 8. First vacuum pump; 9. Second vacuum pump; 10. First air pump; Processing Mechanism: 111. Processing Box; 112. Upper End of Processing Tank; 113. Lower End of Processing Tank; 114. Barrier Ring; 115. Connector; 116. Processing Tank Cover; 117. Stirring Rod; 118. Reinforcing Ring; 119. Water Absorption Steel Pipe; 1191. Protective Component; 1192. Polytetrafluoroethylene Filter Membrane; 1193. Granular Activated Carbon; 1194. First Connecting Water Pipe; 1195. Second Connecting Water Pipe; 1196. Gas Connecting Pipe; 1197. Gas Injection Port; 1198. Second Handle; 1199. First Gear; 11991. Second Gear; 11992. Connecting Frame; 11993. Drive Motor; 11994. Limiting Rod; 12. First distributor; 13. Second distributor; 14. Third distributor; 15. Detector. Detailed Implementation

[0011] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0012] Example 1: Please see Figure 1-10 A soil microbial biological nitrogen content detection device includes a frame 1, with casters 2 at the bottom of the frame 1, a chamber inside the frame 1, two partitions 4 fixedly connected inside the frame 1, the internal chamber of the frame 1 being divided into a first chamber, a second chamber and a third chamber by the partitions 4, a processing mechanism inside the third chamber, and a detector 15 on the frame 1. The processing mechanism includes a processing box 111 and a processing tank upper end 112. A connecting frame 11992 is fixedly connected inside the processing box 111. A processing tank lower end 113 is rotatably connected to the lower side of the processing tank upper end 112. A connecting piece 115 is fixedly connected to the outside of the processing tank lower end 113. A barrier ring 114 is fixedly connected to the outside of the processing tank upper end 112. A limit rod 11994 is fixedly connected to the outside of the processing tank upper end 112.

[0013] A treatment tank cover 116 is bolted to the upper end 112 of the treatment tank. A second handle 1198 is fixedly connected to the treatment tank cover 116. A second connecting water pipe 1195 and a gas connecting pipe 1196 are provided on the treatment tank cover 116. A first connecting water pipe 1194 is connected to the treatment tank cover 116. A third distributor 14 is fixedly connected to the inside of the treatment box 111. An air injection port 1197 is provided on the treatment tank cover 116. A hose is connected to the air injection port 1197. A third vacuum pump is connected to the end of the hose away from the air injection port 1197. The third vacuum pump is connected to an external waste gas treatment device through a pipe. A vacuum valve is provided on the hose.

[0014] The bottom of the processing box 111 is rotatably connected to a first gear 1199. A drive motor 11993 is fixedly connected to the connecting frame 11992. The conveying end of the drive motor 11993 is fixedly connected to a second gear 11991 via a shaft. The outer side of the second gear 11991 meshes with the first gear 1199. The first gear 1199 is fitted with the connecting piece 115. A first distributor 12 is fixedly connected to the connecting frame 11992. A chloroform concentration sensor is installed inside 113.

[0015] A stirring rod 117 is fixedly connected to the lower side of the treatment tank cover 116, a reinforcing ring 118 is fixedly connected to the stirring rod 117, and a water suction pipe 119 is fixedly connected to the treatment tank cover 116, with a drain hole provided on the water suction pipe 119.

[0016] A protective component 1191 is threadedly connected to the lower side of the treatment tank cover 116. The protective component 1191 is installed on the water suction steel pipe 119. A polytetrafluoroethylene filter membrane 1192 is installed inside the protective component 1191. Granular activated carbon 1193 is fixedly connected to the inside of the polytetrafluoroethylene filter membrane 1192. The first connecting water pipe 1194 is connected to the water suction steel pipe 119.

[0017] The first chamber is equipped with a first container 5, a chloroform storage tank 6, and a second container 7. The second chamber is fixedly connected with a first vacuum pump 8, a second vacuum pump 9, and a first air pump 10. The first container 5 is connected to the first vacuum pump 8 through a pipe. The end of the first vacuum pump 8 away from the first container 5 is connected to the third distributor 14 through a pipe. The third distributor 14 is connected to the second connecting water pipe 1195.

[0018] The chloroform storage tank 6 is connected to the first air pump 10 via an air pipe. The first air pump 10 is connected to the second distributor 13 via an air pipe. The second distributor 13 is connected to the gas connection pipe 1196. The end of the first connecting water pipe 1194 away from the treatment tank cover 116 is connected to the second vacuum pump 9. The second vacuum pump 9 is connected to the detector 15 via a pipe. The detector 15 is connected to the second container 7.

[0019] A second distributor 13 is fixedly connected to the connecting frame 11992. The second distributor 13 is connected to the first air pump 10 through an air pipe. A first handle 3 is fixedly connected to the rear side of the frame 1. A heating sleeve is provided on the connecting frame 11992.

[0020] Work process: Take a fresh soil sample, filter it to remove stones, roots and other impurities, and place it in the lower part 113 of the treatment tank. Place the blank control group soil sample in the lower part 113 of another treatment tank. Close the tank lid and tighten the screw. After tightening the screw, start the third vacuum pump to draw the pressure inside the tank to the test value. Close the vacuum valve and the third vacuum pump, and maintain the pressure for five minutes. If the pressure is greater than or equal to the standard value, the seal is qualified. If the pressure is less than the standard value, the alignment of the tank lid needs to be checked again until the seal meets the standard. The verification is then completed. By activating the vacuum extraction valve and starting the third vacuum pump, the pressure inside the lower end 113 of the processing tank is reduced. Then, the vacuum pump and vacuum extraction valve are closed, the first valve is opened, and the first air pump 10 and the first flow sensor are activated. When the first flow sensor detects that the cumulative delivery amount has reached the target value, the first air pump 10 and the first valve at the outlet of the chloroform storage tank 6 are automatically closed to complete the quantitative injection of chloroform. The temperature inside the tank is monitored in real time by the temperature sensor inside the processing box 111 through the heating jacket set on the connecting frame 11992. When the temperature is lower than the set temperature, the heating jacket is automatically activated, and when it is higher than the set temperature, it is stopped to maintain a stable chloroform vapor concentration.

[0021] The upper part 112 of the heating tank is then heated, and the drive motor 11993 is started to drive the second gear 11991 to move. The second gear 11991 drives the first gear 1199 to rotate, thereby driving the lower part 113 of the heating tank to rotate. The rotation of the lower part 113 of the heating tank causes the soil inside the lower part 113 to be continuously stirred with the stirring rod 117, thereby promoting full contact between the steam and the sample. The fumigation lasts for 24 hours. Temperature and pressure are monitored in real time using sensors. Chloroform is then discharged through a third vacuum pump. The first valve is then opened, allowing nitrogen to be evenly distributed through the second distributor 13 and introduced through the gas connection pipe 1196. After chloroform concentration is detected and the nitrogen is completely discharged, the first valve and the first gas pump 10 are closed, and the first vacuum pump 8 is started. The extractant passes sequentially through the first container 5, the first vacuum pump 8, the third distributor 14, the second valve, and the second connecting water pipe 1195. After further stirring, the second vacuum pump 9 and the third valve are started, allowing the extract to be filtered through granular activated carbon 1193 and a polytetrafluoroethylene filter membrane 1192. The extract is then transported to the detector 15 through the first connecting water pipe 1194 for analysis. The detector 15 is a fully automatic Kjeldahl nitrogen analyzer that integrates digestion, distillation, and titration functions. After the extract is transported to the detector through the second vacuum pump 9, the instrument automatically completes digestion, distillation, and titration, calculating the total nitrogen concentration in the extract based on the amount of hydrochloric acid consumed.

[0022] The blank sample processing procedure is exactly the same as that for fumigated samples. Fresh soil of the same batch and quality that has not been fumigated is taken, placed in an independent polytetrafluoroethylene sample cup, and placed in the blank sample isolation slot at the bottom 113 of the processing tank. The subsequent extraction, filtration, and detection steps are carried out simultaneously with the fumigated samples. The detector 15 automatically calculates the difference in nitrogen concentration between the two, which is the final soil microbial biological nitrogen content.

[0023] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0024] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A soil microbial nitrogen content detection device, characterized in that, Includes a frame (1), with casters (2) at the bottom of the frame (1), a chamber inside the frame (1), two partitions (4) fixedly connected inside the frame (1), the chamber inside the frame (1) is divided into a first chamber, a second chamber and a third chamber by the partitions (4), a processing mechanism is provided inside the third chamber, and a detector (15) is provided on the frame (1). The processing mechanism includes a processing box (111) and an upper end (112) of a processing tank. A connecting frame (11992) is fixedly connected inside the processing box (111). A lower end (113) of the processing tank is rotatably connected to the lower end (112). A connecting piece (115) is fixedly connected to the outside of the lower end (113). A barrier ring (114) is fixedly connected to the outside of the upper end (112). A limit rod (11994) is fixedly connected to the outside of the upper end (112).

2. The device for detecting the nitrogen content of soil microorganisms according to claim 1, characterized in that: A treatment tank cover (116) is bolted to the upper end (112) of the treatment tank. A second handle (1198) is fixedly connected to the treatment tank cover (116). A second connecting water pipe (1195) and a gas connecting pipe (1196) are provided on the treatment tank cover (116). A first connecting water pipe (1194) is connected to the treatment tank cover (116). A third distributor (14) is fixedly connected to the inside of the treatment box (111). An air injection port (1197) is provided on the treatment tank cover (116). A hose is connected to the air injection port (1197). A third vacuum pump is connected to the end of the hose away from the air injection port (1197). The third vacuum pump is connected to an external waste gas treatment device through a pipe. A vacuum valve is provided on the hose.

3. The device for detecting the nitrogen content of soil microorganisms according to claim 2, characterized in that: The bottom of the processing tank (111) is rotatably connected to a first gear (1199), and a drive motor (11993) is fixedly connected to the connecting frame (11992). The output end of the drive motor (11993) is fixedly connected to a second gear (11991) via a shaft. The outer side of the second gear (11991) meshes with the first gear (1199). The first gear (1199) is fitted with a connector (115). A first distributor (12) is fixedly connected to the connecting frame (11992). A chloroform concentration sensor is installed inside the lower end (113) of the processing tank.

4. The soil microbial biological nitrogen content detection device according to claim 3, characterized in that: A stirring rod (117) is fixedly connected to the lower side of the treatment tank cover (116), a reinforcing ring (118) is fixedly connected to the stirring rod (117), a water-absorbing steel pipe (119) is fixedly connected to the treatment tank cover (116), and a drain hole is provided on the water-absorbing steel pipe (119).

5. The device for detecting the nitrogen content of soil microorganisms according to claim 4, characterized in that: The lower side of the treatment tank cover (116) is threaded with a protective component (1191). The protective component (1191) is located on the outside of the water-absorbing steel pipe (119), and the inner wall of the protective component (1191) is in contact with the outer wall of the water-absorbing steel pipe (119), covering the drain hole at the lower end of the water-absorbing steel pipe (119). A polytetrafluoroethylene filter membrane (1192) is provided on the inner side of the protective component (1191). Granular activated carbon (1193) is fixedly connected to the inner side of the polytetrafluoroethylene filter membrane (1192). The first connecting water pipe (1194) is connected to the inside of the water-absorbing steel pipe (119).

6. The device for detecting the nitrogen content of soil microorganisms according to claim 2, characterized in that: The first chamber is provided with a first container (5), a chloroform storage tank (6) and a second container (7). The second chamber is fixedly connected with a first vacuum pump (8), a second vacuum pump (9) and a first air pump (10). The first container (5) is connected to the first vacuum pump (8) through a pipe. The end of the first vacuum pump (8) away from the first container (5) is connected to a third distributor (14) through a pipe. The third distributor (14) is connected to a second connecting water pipe (1195).

7. The device for detecting the nitrogen content of soil microorganisms according to claim 6, characterized in that: The chloroform storage tank (6) is connected to the first air pump (10) via an air pipe. The first air pump (10) is connected to the second distributor (13) via an air pipe. The second distributor (13) is connected to the gas connection pipe (1196). The end of the first connecting water pipe (1194) away from the treatment tank cover (116) is connected to the second vacuum pump (9). The second vacuum pump (9) is connected to the detector (15) via a pipe. The detector (15) is connected to the second container (7).

8. The soil microbial biological nitrogen content detection device according to claim 7, characterized in that: A second distributor (13) is fixedly connected to the connecting frame (11992). The second distributor (13) is connected to the first air pump (10) through an air pipe. A first handle (3) is fixedly connected to the rear side of the frame (1). A heating sleeve is provided on the connecting frame (11992).

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