A reagent plate for detecting furazolidone metabolites

Abstract

The utility model discloses a reagent plate of detecting furanazole metabolite product, including reagent plate body and anti -jam mechanism, the surface of reagent plate body is established respectively with sample adding hole and reaction area, the surface fixed setting of reagent plate body has the shelter seat, and the surface of shelter seat is provided with shelter blind frame, the bottom fixed connection of shelter blind frame has the base, and the upper side inlay of shelter blind frame fixed has the clamping seat, the inside fixed of clamping seat is inserted with rubber seat. This reagent plate of detecting furanazole metabolite product, sample solution of dropper bottom enters to the inside of sample adding hole, and the sample adding hole, reaction area outside of reagent plate body is wrapped shelter blind frame, and shelter blind frame can prevent dust interference to nitrocellulose membrane of sample adding hole, reaction area bottom, can also insulate the interference of indoor light to the detection result, improve the accuracy of reagent plate body detection result.

Description

Technical Field

[0001] This utility model relates to the field of biological detection, specifically a reagent plate for detecting furazolidone metabolites. Background Technology

[0002] Furazolidone is a nitrofuran antibiotic that was once used to treat intestinal infections in animals. However, due to serious safety risks, it has been banned for use in food animal farming by many countries (including China). The purpose of testing is to ensure food safety and human health. The core principle of the reagent plate for detecting furazolidone metabolites is colloidal gold immunochromatography (GICA), based on the specific binding reaction of antigen and antibody. The reagent plate is pre-coated with specific antibodies against furazolidone metabolites (mainly AOZ, i.e., aminourea) and artificially synthesized AOZ antigen. AOZ in the sample to be tested (such as animal tissue or urine) binds to the colloidal gold-labeled antibody, forming an "antibody-AOZ" complex. When the complex moves to the test line with the chromatography solvent, if the AOZ content in the sample is too high, it will competitively inhibit the binding of the "colloidal gold antibody" to the AOZ antigen at the test line, resulting in no color development (positive). If the AOZ content is low or absent, the "colloidal gold antibody" binds to the antigen at the test line, and the test line develops color (negative), thus determining whether furazolidone metabolites remain in the sample. In the detection of furazolidone metabolites, the reagent plate contains a nitrocellulose membrane. The nitrocellulose membrane is essentially a three-dimensional network structure composed of countless interwoven micropores. These micropores generate strong capillary action, allowing the sample solution to flow continuously, stably, and unidirectionally from the sample application point (S end) to the other end (the direction of the absorbent paper), much like an oil wick absorbing oil. Therefore, the quality of the nitrocellulose membrane micropores (pore size, uniformity, and protein binding capacity) directly determines the sensitivity, accuracy, chromatography speed, and batch-to-batch consistency of the reagent plate, and is crucial to the success of immunochromatography. In actual testing, the reaction zone and sample wells on the reagent plate are directly exposed to the air. Dust particles in the air may clog the micropores of the nitrocellulose membrane on the reagent plate, hindering chromatographic flow and leading to chromatography failure (the solution cannot flow) or the formation of nonspecific bands (false positives). Utility Model Content

[0003] The purpose of this invention is to provide a reagent plate for detecting furazolidone metabolites, so as to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, a reagent plate for detecting furazolidone metabolites is provided, comprising a reagent plate body and an anti-interference mechanism. The surface of the reagent plate body is provided with a sample application hole and a reaction zone, respectively. A shielding seat is fixedly disposed on the surface of the reagent plate body, and a shielding blind frame is disposed on the surface of the shielding seat. A base is fixedly connected to the bottom of the shielding blind frame, and a card holder is embedded and fixedly disposed on the upper side of the shielding blind frame. A rubber seat is fixedly inserted inside the card holder.

[0005] Furthermore, the anti-interference mechanism also includes a positioning groove, a positioning block, and a viewing window, and the viewing window is a rectangular structure made of acrylic material, while the viewing window is fixed on the side wall of the blind frame.

[0006] Furthermore, the shielding base is rectangular, and four sets of positioning grooves are evenly provided on the shielding base. Four sets of positioning blocks are evenly installed on the bottom of the base, and both the positioning blocks and the positioning grooves are arc-shaped.

[0007] Furthermore, the positioning block is inserted into the inside of the positioning groove, and the depth of the positioning groove is equal to the height of the positioning block. The blind frame covers the reagent plate body and is positioned and installed through four sets of positioning grooves and positioning blocks.

[0008] Furthermore, both the card holder and its internal rubber seat are cylindrical, and a through hole is provided through the middle of the rubber seat, with the through hole being positioned opposite to the sample application hole.

[0009] Furthermore, the shielding blind frame has a cuboid structure and covers the outer surface of the sample application hole and the reaction zone. The shielding blind frame is a detachable structure on the reagent plate body.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows: the rubber seat is expanded, and the sample solution at the bottom of the dropper enters the interior of the sample application hole. The sample application hole and the outer side of the reaction zone on the reagent plate body are wrapped with a shielding blind frame. The shielding blind frame can prevent dust interference on the nitrocellulose membrane at the bottom of the sample application hole and the reaction zone, and can also isolate the interference of indoor light on the detection results, thereby improving the accuracy of the detection results of the reagent plate body. By blocking indoor light, the shielding blind frame provides a light-proof dark room environment for the entire immunochromatographic reaction, which maximizes the protection of the stability of these biological reagents, ensures that the reaction is carried out under optimal conditions, and improves the reliability of the detection results. Attached Figure Description

[0011] Figure 1 This is a front view schematic diagram of the structure of this utility model; Figure 2 This is a bottom view of the structure of this utility model; Figure 3 This is an external view of the structure of this utility model; Figure 4This is a schematic diagram of the anti-interference mechanism of this utility model. Figure 5 This is a cross-sectional view of the structure of this utility model.

[0012] The following numbers are labeled in the diagram: 100, reagent plate body; 11, sample dispensing hole; 12, reaction zone; 200, anti-interference mechanism; 21, shielding seat; 22, positioning groove; 23, shielding blind frame; 24, base; 25, positioning block; 26, viewing window; 27, card holder; 271, rubber seat; 272, perforation. Detailed Implementation

[0013] Please see Figure 1-5 This utility model provides a reagent plate for detecting furazolidone metabolites, including a reagent plate body 100 and an anti-interference mechanism 200. The surface of the reagent plate body 100 is provided with a sample application hole 11 and a reaction zone 12. A shielding seat 21 is fixedly provided on the surface of the reagent plate body 100, and a shielding blind frame 23 is provided on the surface of the shielding seat 21. A base 24 is fixedly connected to the bottom of the shielding blind frame 23, and a card seat 27 is embedded and fixed on the upper side of the shielding blind frame 23. A rubber seat 271 is fixedly inserted inside the card seat 27.

[0014] The anti-interference mechanism 200 also includes a positioning groove 22, a positioning block 25 and a viewing window 26, and the viewing window 26 is a rectangular structure made of acrylic material, and the viewing window 26 is fixed on the side wall of the blind frame 23.

[0015] The shielding base 21 is rectangular, and four sets of positioning grooves 22 are evenly provided on the shielding base 21. Four sets of positioning blocks 25 are evenly installed on the bottom of the base 24, and both the positioning blocks 25 and the positioning grooves 22 are arc-shaped.

[0016] The positioning block 25 is inserted into the inside of the positioning groove 22, and the depth of the positioning groove 22 is equal to the height of the positioning block 25. The blind frame 23 covers the reagent plate body 100 and is positioned and installed through four sets of positioning grooves 22 and positioning blocks 25.

[0017] Both the card holder 27 and its internal rubber seat 271 are cylindrical, and a through hole 272 is provided through the middle of the rubber seat 271. The through hole 272 is positioned opposite to the sample feeding hole 11.

[0018] In a preferred embodiment, the shielding blind frame 23 has a cuboid structure and covers the outer surface of the sample application hole 11 and the reaction zone 12. The shielding blind frame 23 is a detachable structure on the reagent plate body 100.

[0019] like Figure 1-4As shown: The elastic design of the rubber seat 271 allows the dropper to be easily inserted and centered, forming a natural guide channel; ensuring that even inexperienced operators can accurately introduce the sample solution into the sample well 11, greatly reducing the risk of solution splashing or chromatography failure due to sample deviation; when the rubber seat 271 is opened, its inner wall fits tightly against the dropper, forming a temporary sealing structure; the seal effectively prevents accidental leakage, backflow, or evaporation of the solution during sample addition due to shaking or surface tension, ensuring the consistency and accuracy of the added sample volume, which is the basis for obtaining reliable test results; it creates a dust-free reaction environment and eliminates particulate interference; the blind frame 23 completely encloses the sample well 11 and the reaction zone 12, effectively preventing suspended particles such as dust, hair, and fibers in the air from falling into the sample well 11 or directly adhering to the nitrocellulose membrane at the bottom of the reaction zone 12; contaminants may clog the micropores of the membrane, leading to abnormal chromatography speed and flow interruption, avoiding false positives or invalid results; Proteins, antibodies, antigens, and colloidal gold markers on nitrocellulose membranes are sensitive to light, especially ultraviolet light. Prolonged exposure to strong light may cause their biological activity to decrease, thereby reducing detection sensitivity and creating a risk of false negatives. The blind box 23 provides a dark environment for the entire immunochromatographic reaction by blocking indoor light, which maximizes the protection of the stability of these biological reagents and ensures that the reaction is carried out under optimal conditions. Working principle: When detecting furazolidone metabolites in tissue samples, a dropper is used to draw the sample solution and insert it into the rubber seat 271 inside the rubber seat 271. The rubber seat 271 is opened, and the sample solution at the bottom of the dropper enters the sample application hole 11. The sample application hole 11 and the reaction area 12 on the reagent plate body 100 are covered by a shielding blind frame 23. The shielding blind frame 23 can prevent dust interference on the nitrocellulose membrane at the bottom of the sample application hole 11 and the reaction area 12, and can also isolate the interference of indoor light on the detection results, thereby improving the accuracy of the detection results of the reagent plate body 100. After standing, the shielding blind frame 23 is removed, and the four sets of positioning blocks 25 detach from the four sets of positioning grooves 22. By observing the information inside the reaction area 12, it can be determined whether furazolidone metabolites are present in the sample solution.

Claims

1. A reagent plate for detecting furazolidone metabolites, comprising a reagent plate body (100) and an anti-interference mechanism (200), wherein the surface of the reagent plate body (100) is respectively provided with a sample application hole (11) and a reaction zone (12); characterized in that: A shielding seat (21) is fixedly provided on the surface of the reagent plate body (100), and a shielding blind frame (23) is provided on the surface of the shielding seat (21); a base (24) is fixedly connected to the bottom of the shielding blind frame (23), and a card seat (27) is fixedly embedded on the upper side of the shielding blind frame (23), and a rubber seat (271) is fixedly inserted inside the card seat (27).

2. The reagent plate for detecting furazolidone metabolite according to claim 1, wherein: The anti-interference mechanism (200) also includes a positioning groove (22), a positioning block (25) and a window (26), and the window (26) is a rectangular structure made of acrylic material, and the window (26) is fixed on the side wall of the blind frame (23).

3. The reagent plate for detecting furazolidone metabolite according to claim 2, wherein: The shielding seat (21) is rectangular, and four sets of positioning grooves (22) are evenly provided on the shielding seat (21). Four sets of positioning blocks (25) are evenly installed on the bottom of the base (24), and the positioning blocks (25) and positioning grooves (22) are both arc-shaped.

4. The reagent plate for detecting furazolidone metabolite according to claim 3, characterized by: The positioning block (25) is inserted into the inside of the positioning groove (22), and the depth of the positioning groove (22) is equal to the height of the positioning block (25). The blind frame (23) covers the reagent plate body (100) and is positioned and installed through four sets of positioning grooves (22) and positioning blocks (25).

5. The reagent plate for detecting furazolidone metabolites according to claim 1, characterized in that: The card holder (27) and its internal rubber seat (271) are both cylindrical, and a through hole (272) is provided through the middle of the rubber seat (271), and the through hole (272) is arranged opposite to the sample feeding hole (11).

6. The reagent plate for detecting furazolidone metabolite according to claim 4, wherein: The shielding blind frame (23) has a cuboid structure and covers the outer surface of the sample application hole (11) and the reaction zone (12). The shielding blind frame (23) is a detachable structure on the reagent plate body (100).

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