Sample addition detection device

Abstract

The utility model discloses a kind of sample adding detection devices, belong to the technical field of sample adding, inspection, wherein, including box, auxiliary sample adding element, box has accommodating groove inside, the front and rear ends of box are all with notches, two notches are formed sample inlet, sample outlet respectively, sample inlet, accommodating groove, sample outlet are sequentially communicated;Auxiliary sample adding element includes frame, at least two push plates, frame is installed on box;Push plate is slidably arranged in frame, and push plate and frame or two push plates form sample adding through opening between two push plates;One push plate has first moving position, second moving position, on first moving position, two push plates form sample adding through opening between two push plates;On second moving position, one push plate and frame form sample adding through opening between two push plates.The end of one push plate to sample outlet moves, mark sample adding hole on one side of sample adding through opening, sample adding hole on the other side of sample adding through opening and below is to be added, solve the problem of sample adding leakage.

Description

Technical Field

[0001] This utility model relates to the technical field of sample addition and testing, and in particular to a sample addition and testing device. Background Technology

[0002] Microplates are small in size, and their visibility can be easily obstructed when used in a biosafety cabinet. Personnel must manually memorize the sample placement positions, and due to visual distractions or inattention, the probability of missing wells is high. Missing wells can lead to incomplete biochemical reactions (e.g., some wells lacking samples, affecting identification conclusions) or missing drug sensitivity test data (e.g., wells without samples of a certain drug concentration leading to incorrect MIC value determination), directly impacting the accuracy and reliability of the test results. Utility Model Content

[0003] The purpose of this invention is to improve the problem of existing sample addition and testing devices that are prone to missing samples, thus affecting the accuracy of test results, and to provide a sample addition and testing device.

[0004] The technical solutions for achieving the above objectives include the following:

[0005] A sample addition and detection device includes: a housing and an auxiliary sample addition component. The housing has a receiving groove, and both the front and rear ends of the housing have notches, which respectively form a sample inlet and a sample outlet. The sample inlet, the receiving groove, and the sample outlet are connected in sequence. The auxiliary sample addition component includes a frame and at least two push plates, and the frame is mounted on the housing.

[0006] The pusher plate is slidably disposed within the frame, and a sample dispensing port is formed between the pusher plate and the frame or between two pusher plates; wherein one of the pusher plates has a first moving position and a second moving position, wherein in the first moving position, the sample dispensing port is formed between the two pusher plates; and in the second moving position, the sample dispensing port is formed between one of the pusher plates and the frame.

[0007] In one embodiment, the sample addition and detection device further includes a light strip installed in a receiving groove, the bottom wall of which has a reflective film.

[0008] The frame is rotatably connected to the housing. When the frame rotates to the upper end of the housing, the push plate is positioned above the light strip.

[0009] In one embodiment, the sample addition and detection device also has multiple control knobs and multiple control circuits. The number of control knobs, control circuits, and light strips corresponds to each other. Multiple control knobs are all located on the side wall of the housing, and each control knob is electrically connected to the light strip through the control circuit.

[0010] In one embodiment, the length direction of the light strip intersects with the length direction of the push plate.

[0011] In one embodiment, the push plate has multiple pieces, both ends of which are slidably engaged with the frame, and the multiple push plates are arranged in parallel.

[0012] In one embodiment, the frame has a guide block that extends along the moving direction of the push plate, and the push plate has a guide groove that slides with the guide block.

[0013] In one embodiment, the frame further includes a barrier, the guide block is mounted on the barrier, a limiting groove is formed between the guide block and one side of the barrier, and the push plate is at least partially located within the limiting groove.

[0014] In one embodiment, the sample addition and detection device further includes a hinge and a cover, the cover being rotatably connected to a frame or box via a hinge, and the frame being rotatably connected to the box or cover via a hinge.

[0015] In one embodiment, the sample addition detection device further includes a multi-well plate, one end of which passes through the sample inlet and is located on the receiving groove. The two ends of the multi-well plate are located at the two notches of the box body. The multi-well plate has multiple sample addition holes arranged in an array.

[0016] In one embodiment, both the perforated plate and the pusher plate are made of transparent material.

[0017] The technical solution provided by this utility model has the following advantages and effects:

[0018] On one hand, when it is necessary to add samples to the multi-well plate, the two ends of the multi-well plate are placed at the inlet and outlet, respectively, with the multi-well plate located in the receiving tank and the frame positioned above it. When it is necessary to add samples to the first row of wells, one of the push plates is moved to a second moving position, creating a sample dispensing opening between the first push plate and the frame. The pipette passes through the dispensing opening to add samples to the first row of wells. After the first row of wells has been filled, one of the push plates is moved to the first moving position, creating a sample dispensing opening between the two push plates. The pipette passes through the dispensing opening to add samples to the second row of wells; and so on, thus completing the sample addition operation of the multi-well plate.

[0019] On the other hand, in actual use, the sample addition detection device can use one of the push plates to move towards one end of the sample outlet to mark the sample addition holes on one side of the sample addition port as those that have been sampled, the sample addition holes on the other side of the sample addition port as those that have not been sampled, and the sample addition holes below the sample addition port as those to be sampled, thus solving the problem of missed sample addition and affecting the accuracy of the test results. Attached Figure Description

[0020] The accompanying drawings illustrate specific examples of the technical solutions described in this utility model, and together with the detailed embodiments, form part of the specification, serving to explain the technical solutions, principles, and effects of this utility model.

[0021] Unless otherwise specified or defined, the same reference numerals in different figures represent the same or similar technical features, and different reference numerals may be used to represent the same or similar technical features.

[0022] Figure 1 This is a schematic diagram of the closed state of the sample addition and detection device in one embodiment of this utility model;

[0023] Figure 2 This is a schematic diagram of the open state of the sample addition and detection device in one embodiment of this utility model. Figure 1 ;

[0024] Figure 3 This is a schematic diagram of the open state of the sample addition and detection device in one embodiment of this utility model. Figure 2 ;

[0025] Figure 4 This is a cross-sectional view of the push plate and frame in one embodiment of the present invention;

[0026] Figure 5 This is a schematic diagram of a perforated plate in one embodiment of the present invention;

[0027] Explanation of reference numerals in the attached figures:

[0028] 100. Sample addition and testing device; 1. Box body; 11. Sample inlet; 12. Sample outlet; 13. LED strip; 14. Receiving tank; 15. Reflective film; 101. Switch knob; 102. Control knob; 2. Cover; 3. Auxiliary sample addition component; 31. Push plate; 311. Guide groove; 32. Frame; 321. Enclosure; 322. Limiting groove; 33. Sample addition port; 34. Guide block; 4. Hinge; 5. Perforated plate; 51. Sample addition hole. Detailed Implementation

[0029] To facilitate understanding of this utility model, the specific embodiments of this utility model will be described in more detail below with reference to the accompanying drawings.

[0030] Unless otherwise specified or defined, the terms "first," "second," etc., used in this document are for distinguishing names only and do not represent a specific number or order.

[0031] Unless otherwise stated or defined, the term “and / or” as used herein includes any and all combinations of one or more of the associated listed items.

[0032] It should be noted that when a component is considered "fixed" to another component, it can be directly fixed to the other component or there can be an intervening component; when a component is considered "connected" to another component, it can be directly connected to the other component or there can be an intervening component; when a component is considered "mounted" on another component, it can be directly mounted on the other component or there can be an intervening component; when a component is considered "placed" on another component, it can be directly placed on the other component or there can be an intervening component.

[0033] This utility model proposes a sample addition and detection device 100, such as... Figures 1 to 5 As shown, the device includes a housing 1 and an auxiliary sample feeding component 3. The housing 1 has a receiving groove 14 inside. Both the front and rear ends of the housing 1 have notches, which respectively form a sample inlet 11 and a sample outlet 12. The sample inlet 11, the receiving groove 14, and the sample outlet 12 are connected in sequence. The auxiliary sample feeding component 3 includes a frame 32 and at least two push plates 31. The frame 32 is installed on the housing 1. The push plates 31 are slidably disposed in the frame 32, and a sample feeding passage 33 is formed between the push plates 31 and the frame 32 or between the two push plates 31. One of the push plates 31 has a first moving position and a second moving position. In the first moving position, a sample feeding passage 33 is formed between the two push plates 31. In the second moving position, a sample feeding passage 33 is formed between one of the push plates 31 and the frame 32.

[0034] Specifically, when it is necessary to add samples to the multi-well plate 5, the two ends of the multi-well plate 5 are placed at the inlet 11 and the outlet 12, with the multi-well plate 5 located inside the receiving tank 14, and the frame 32 positioned above the multi-well plate 5. When it is necessary to add samples to the first row of the multi-well plate 5, one of the push plates 31 is moved to a second moving position, forming a sample dispensing port 33 between the first push plate 31 and the frame 32. The pipette passes through the sample dispensing port 33 to add samples to the sample wells 51 of the first row. After the sample wells 51 of the first row are filled, one of the push plates 31 is moved to the first moving position, forming a sample dispensing port 33 between the two push plates 31. The pipette passes through the sample dispensing port 33 to add samples to the sample wells 51 of the second row; and so on, to achieve the sample dispensing operation of the multi-well plate 5.

[0035] Furthermore, in actual use, the sample addition and detection device 100 can use one of the push plates 31 to move towards one end of the sample outlet 12, thereby marking the sample addition holes 51 that have been sampled on one side of the sample addition port 33, the sample addition holes 51 that have not been sampled on the other side of the sample addition port 33, and the sample addition holes 51 below the sample addition port 33 that are to be sampled, thus solving the problem of missed sample addition and affecting the accuracy of the test results.

[0036] In some embodiments, the sample addition and detection device 100 also includes a light strip 13, which is installed in a receiving groove 14. The bottom wall of the receiving groove 14 has a reflective film 15. The frame 32 is rotatably connected to the housing 1. When the frame 32 is rotated to the upper end of the housing 1, the push plate 31 is positioned above the light strip 13. When it is necessary to observe the biochemical reaction results, the push plate 31 can be removed above the light strip 13. Therefore, this rotatable arrangement of the frame 32 and the housing 1 can prevent the multi-well plate 5 from being missed and also facilitate the observation of the sample incubation status of the multi-well plate 5.

[0037] Specifically, after sample addition, it is necessary to visually determine the positive and negative results of the wells (biochemical reaction results) and the growth status of the drug-sensitive broth culture (for calculating MIC values). Manual interpretation is susceptible to visual fatigue and color recognition errors (e.g., different personnel may have different criteria for judging "weak positive"), and it is impossible to standardize and quantify the well status, resulting in poor consistency of results. Especially in large-scale testing, the accumulation of errors can significantly reduce the quality of testing.

[0038] After all samples have been added to the multi-well plate 5, open the auxiliary sample loading device 3 to remove the multi-well plate 5, seal it with sealing tape, and place it in the corresponding environment for incubation. After incubation (data reading), place the multi-well plate 5 back into the sample loading and detection device 100. By activating the light strip 13, the multi-well plate 5 is illuminated by light and the reflective film 15, making it easy to identify and record the values ​​of biochemical reactions and drug concentration growth, reducing manual operation steps, shortening the detection cycle, and improving work efficiency. Therefore, this sample loading and detection device 100 has the functions of avoiding missed additions and detecting biochemical reactions.

[0039] Preferably, the sample addition and detection device 100 also has multiple control knobs 102 and multiple control circuits. The number of control knobs 102, control circuits, and light strips 13 corresponds to each other. Multiple control knobs 102 are all located on the side wall of the housing 1, and each control knob 102 is electrically connected to the light strip 13 through a control circuit. Specifically, the control knobs 102 control the starting or stopping of the light strip 13 through the control circuit, and the brightness of the light strip 13 can be adjusted when the control knobs 102 are rotated. Different samples show significant changes in biochemical reactions and drug concentration growth values ​​under different brightness levels, which can further improve the flexibility of its detection.

[0040] Preferably, the length direction of the light strip 13 intersects the length direction of the push plate 31. Specifically, one light strip 13 can observe the biochemical reactions and drug concentration growth values ​​of the samples on a row of sample wells 51. Each control knob 102 controls the corresponding light strip 13 through a corresponding control circuit. When interpreting the results, the switch knob 101 is turned on, and the brightness of the light source is adjusted. When it is necessary to read the results in a single row, the control knob 102 of a single light strip 13 can be controlled, so that the values ​​of biochemical reactions and drug concentration growth wells in the entire well plate 5 can be easily interpreted and identified under the illumination and background of the reflective film 15.

[0041] Preferably, the pusher plate 31 has multiple pieces, both ends of which are slidably engaged with the frame 32, and the multiple pusher plates 31 are arranged in parallel. Specifically, the number of pusher plates 31 corresponds to the number of rows of the multi-hole plate 5. To be precise, the number of pusher plates 31 corresponds to the number of rows of the multi-hole plate 5. After each row of sample holes 51 is filled, one pusher plate 31 is moved to allow multiple rows of sample holes 51 to be filled sequentially.

[0042] Preferably, the frame 32 has a guide block 34 that extends along the moving direction of the push plate 31, and the push plate 31 has a guide groove 311 that slides with the guide block 34. Specifically, the push plate 31 slides with the guide block 34 on the frame 32 through the guide groove 311, which limits the moving direction of the push plate 31 and improves the stability of the push plate 31 moving on the frame 32.

[0043] Preferably, the frame 32 also has a barrier 321, and a guide block 34 is mounted on the barrier 321. A limiting groove 322 is formed between the guide block 34 and one side of the barrier 321, and the push plate 31 is at least partially located within the limiting groove 322. Specifically, this limiting groove 322 is equivalent to another guide groove 311, allowing the end of the push plate 31 to slide in the limiting groove 322, further restricting the movement direction of the push plate 31 and improving the movement stability of the push plate 31.

[0044] Preferably, the sample addition and detection device 100 further includes a hinge 4 and a cover 2. The cover 2 is rotatably connected to the frame 32 or the box 1 via the hinge 4, and the frame 32 is rotatably connected to the box 1 or the cover 2 via the hinge 4. In this embodiment, the hinge 4 is an existing hardware component that can be purchased from the market. The hinge 4 has three rotating plates, one of which is fixed to the side wall of the box 1, another is fixed to the frame 32, and the remaining one is fixed to the cover 2. The three rotating plates are rotatably connected via a rotating shaft to achieve a rotatable connection between the cover 2, the frame 32, and the box 1.

[0045] Preferably, the sample loading and detection device 100 further includes a multi-well plate 5, one end of which passes through the sample inlet 11. The multi-well plate 5 is located on the receiving tank 14, and both ends of the multi-well plate 5 are located at the two notches of the housing 1. The multi-well plate 5 has multiple sample loading holes 51, which are arranged in an array. Specifically, the multi-well plate 5 is placed at the notch of the housing 1, and the sample loading holes 51 on the multi-well plate 5 are arranged in an array, so that the multiple sample loading holes 51 are arranged in multiple rows and columns. Samples or reagents are added into the sample loading holes 51 through a pipette. The multi-well plate 5 is easy to remove or install from the housing 1, further facilitating sample loading and incubation.

[0046] Preferably, both the multi-well plate 5 and the pusher plate 31 are made of transparent material. Specifically, after the light strip 13 is turned on, the biochemical reaction results of the sample in the sample well 51 can be observed because the multi-well plate 5 is transparent. Since the pusher plate 31 is also made of transparent material, it is not necessary to move the pusher plate 31 when observing the sample.

[0047] When referencing drawings, new features are explained. To avoid redundant references to drawings that would make the description less concise, features already described will not be referenced again on the drawings if the description is clear.

[0048] The purpose of the above embodiments is to reproduce and derive the technical solution of this utility model by way of example, and to fully describe the technical solution, purpose and effect of this utility model. The purpose is to enable the public to have a more thorough and comprehensive understanding of the disclosed content of this utility model, and it is not intended to limit the protection scope of this utility model.

[0049] The above embodiments are not an exhaustive list based on the present invention, and there may be other embodiments not listed. Any substitutions and improvements made without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A sample loading detection device, characterized by, include: The box body and the auxiliary sample feeding component are provided. The box body has a receiving groove, and there are notches at both the front and rear ends of the box body. The two notches form a sample inlet and a sample outlet, respectively. The sample inlet, the receiving groove, and the sample outlet are connected in sequence. The auxiliary sample feeding component includes a frame and at least two push plates. The frame is installed on the box body. The pusher plate is slidably disposed within the frame, and a sample dispensing port is formed between the pusher plate and the frame or between two pusher plates; wherein one of the pusher plates has a first moving position and a second moving position, wherein in the first moving position, the sample dispensing port is formed between the two pusher plates; and in the second moving position, the sample dispensing port is formed between one of the pusher plates and the frame.

2. The sample application detection device of claim 1, wherein The sample addition and detection device also has a light strip, which is installed in a receiving groove, and the bottom wall of the receiving groove has a reflective film; The frame is rotatably connected to the housing. When the frame rotates to the upper end of the housing, the push plate is positioned above the light strip.

3. The sample application detection device of claim 2, wherein, The sample addition and detection device also has multiple control knobs and multiple control circuits. The number of control knobs, control circuits and light strips corresponds to each other. Multiple control knobs are all set on the side wall of the box, and each control knob is electrically connected to the light strip through the control circuit.

4. The sample application detection device of claim 3, wherein The length direction of the light strip intersects with the length direction of the push plate.

5. The sample application detection device of claim 1, wherein, The push plate consists of multiple pieces, both ends of which are slidably engaged with the frame, and the multiple push plates are arranged in parallel with each other.

6. The sample addition and detection device as described in claim 5, characterized in that, The frame has a guide block that extends along the moving direction of the push plate, and the push plate has a guide groove that slides with the guide block.

7. The sample addition and detection device as described in claim 6, characterized in that, The frame also has a enclosure, the guide block is mounted on the enclosure, a limiting groove is formed between the guide block and one side of the enclosure, and the push plate is at least partially located in the limiting groove.

8. The sample addition and detection device according to any one of claims 1 to 7, characterized in that, The sample addition and detection device also has a hinge and a cover. The cover is rotatably connected to the frame or box via the hinge, and the frame is rotatably connected to the box or cover via the hinge.

9. The sample addition and detection device according to any one of claims 1 to 7, characterized in that, The sample loading and detection device also includes a multi-well plate, one end of which passes through the sample inlet and is located on the receiving groove. The two ends of the multi-well plate are located at the two notches of the box body. The multi-well plate has multiple sample loading holes, which are arranged in an array.

10. The sample addition and detection device as described in claim 9, characterized in that, Both the perforated plate and the pusher plate are made of transparent material.

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