A plant tissue culture apparatus

Abstract

The utility model discloses a plant tissue culture device relates to plant tissue culture technical field. The device includes incubator, and the inside lower extreme fixed connection bottom plate of incubator, and the fixed frame is fixedly connected to the middle position of bottom plate upper end, and the rotatory lever is equipped in fixed frame, and the rotatory lever is connected with motor through bevel gear set, and the rotatory lever upper end fixed culture frame, and culture frame is set up with the placement groove of rubber convex ball and the collecting groove, the water storage frame is equipped on the upper end of incubator, and the water storage frame is connected with the spray head through the water pipe, and the adjustable height's lighting lamp is equipped in the inside upper end, and the front surface hinged closed door with transparent observation window. The device can realize plant tissue even light receiving, accurate water replenishing, is convenient for observation and maintenance, improves culture effect and efficiency, is applicable to the scale culture of various plant tissues.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, specifically a plant tissue culture device. Background Technology

[0002] Plant tissue culture is an important technique in the field of biotechnology. It achieves rapid propagation and variety improvement by culturing plant tissues or cells in a sterile environment. However, existing plant tissue culture devices have several shortcomings: First, uneven light exposure within the culture container leads to significant differences in growth rates among different tissues, affecting culture quality. Second, watering methods are mostly manual spraying or general irrigation, making it difficult to precisely control the amount and area of ​​water, easily resulting in localized waterlogging or insufficient watering. Furthermore, excess water is difficult to drain, increasing the risk of bacterial growth. Third, the fixed height of the lighting equipment makes it impossible to adjust according to the different growth stages of the plant tissues, resulting in poor applicability. Fourth, observing the internal conditions during culture requires opening the device, which can disrupt the sterile environment and increase the risk of contamination. Therefore, there is an urgent need for a plant tissue culture device that can solve these problems to improve the efficiency and quality of plant tissue culture. Utility Model Content

[0003] The purpose of this invention is to provide a plant tissue culture device to solve the problems of uneven light exposure, inaccurate hydration, non-adjustable light intensity, and inconvenient observation in existing technologies. To achieve the above objective, this invention provides the following technical solution: A plant tissue culture device includes a culture chamber. A base plate is fixedly connected to the lower end of the culture chamber, providing stable support for the internal components. A fixed frame is fixedly connected to the upper middle position of the base plate, defining the installation position of a rotating rod to ensure its rotational stability. A rotating rod is positioned in the middle of the fixed frame. A bevel gear set is sleeved on the outer side of the rotating rod, transmitting motor power in a specific direction. The right side of the bevel gear set is connected to the rotating shaft of the motor, which provides power for the rotation of the rotating rod. A protective cover is provided on the outer side of the motor, effectively protecting it from external impact damage and ensuring heat dissipation during operation through ventilation holes.

[0004] A culture frame is fixedly connected to the upper end of the rotating rod. The culture frame provides a space for plant tissue culture, and evenly spaced placement slots are provided on the culture frame. The placement slots are used to place plant tissue culture containers individually. Rubber convex balls fixedly connected to the inner wall of the placement slots can clamp and fix the culture containers to prevent shaking during rotation. The lower end of the placement slots has evenly spaced openings to facilitate the flow of excess water into the collection slot. The lower inner end of the culture frame has a collection slot to collect excess water seeping out of the placement slots. The lower inner end of the collection slot has a sloping structure to facilitate the centralized drainage of water. A rubber sealing plug is inserted into the lower right side of the culture frame to facilitate the periodic cleaning of water accumulation in the collection slot.

[0005] A control box is fixedly installed on the lower left side of the incubator. The control box is used to control the start and stop of the motor, the speed, and the switching and brightness of the lighting.

[0006] A water storage frame is fixedly connected to the upper end of the incubator. The water storage frame is used to store the water or nutrient solution required for cultivation. A water inlet is opened on the upper left side of the water storage frame to facilitate the replenishment of water or nutrient solution into the water storage frame. The rubber sleeve on the upper outer side can enhance the sealing and prevent impurities from entering. The lower inner end of the water storage frame is designed with an inverted V-shaped structure to facilitate the complete introduction of internal water into the water pipe. Water pipes are fixedly connected to both the left and right ends of the water storage frame. The water pipes are used to transport water or nutrient solution, and the other end of the water pipes is fixedly connected to a nozzle. The nozzle is fixedly installed in the middle of the left and right sides inside the incubator. The upper end of the water pipe is designed with an inclined structure, and the lower end is designed with an opposing inclined structure. With the inclined spray nozzle, water or nutrient solution can be evenly sprayed onto the plant tissue in the incubator to achieve precise water replenishment.

[0007] The upper part of the incubator is equipped with a light source to provide the necessary illumination for plant tissue growth. Connecting blocks are fixedly connected to both sides of the light source, and connecting rods are fixedly connected to the other end of each connecting block. The connecting rods are inserted into the left and right ends of the incubator. The connecting blocks are T-shaped and movably engage with the incubator. T-shaped grooves are provided on both sides of the incubator to ensure the stability of the light source during vertical movement. The connecting rods are U-shaped, and grooves are provided on the incubator to mate with them. A locking bolt is inserted into the connecting rod on the right side, and locking slots are evenly distributed on the incubator to mate with the locking bolts. By adjusting the position of the connecting rods and securing them with the locking bolts, the height of the light source can be flexibly adjusted.

[0008] The front surface of the incubator is hinged with closed doors on both the left and right sides. The front surface of the closed doors is provided with transparent observation windows, which facilitates the observation of the growth of the plant tissue inside without opening the device. The bottom plate is evenly provided with openings. The lower end of the incubator is designed with a sloping structure. A rubber plug is inserted in the middle of the lower right end of the incubator to facilitate the drainage of water that may accidentally drip inside the device and keep the inside dry and clean.

[0009] Compared with the prior art, the beneficial effects of this utility model are: This invention features a rotating rod, a bevel gear set, and a motor, which drive the culture frame to rotate at a uniform speed. The culture frame then drives the plant tissues in the placement tank to rotate synchronously, ensuring that the plant tissues in each culture container receive uniform illumination from the lighting lamp. This avoids growth differences caused by uneven light exposure and significantly improves the culture quality of the plant tissues. At the same time, the motor speed can be adjusted through a control box to suit the light uniformity requirements of different plant tissues.

[0010] This utility model is equipped with a lighting lamp, a connecting block, and a connecting rod. The lighting lamp can be moved up and down by the cooperation of the connecting block and the connecting rod. This allows for precise adjustment of the lighting lamp's height according to the height and light requirements of different growth stages of plant tissue, enhancing the applicability of the device. Furthermore, the design of the locking bolt and locking groove ensures that the lighting lamp remains stable after adjustment, preventing shaking from affecting the lighting effect.

[0011] This invention achieves precise spraying of water and nutrient solution through the combination of a water storage frame, water pipe, and nozzle. The inverted V-shaped water storage frame ensures full utilization of water, while the angled design of the water pipe and the angled nozzle makes the spraying range more uniform, avoiding local water accumulation or insufficient water. At the same time, the drainage structure at the bottom of the collection tank and incubator facilitates the timely removal of excess water, reduces bacterial growth, and ensures the cleanliness of the cultivation environment. Attached Figure Description To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a cross-sectional structural diagram of the present invention; Figure 3 This is an exploded structural diagram of the components of the lighting lamp of this utility model; Figure 4 This is a cross-sectional view of the fixing frame of this utility model; Figure 5 This is a cross-sectional view of the culture frame of this utility model.

[0013] In the diagram: 1. Incubator; 2. Base plate; 3. Fixing frame; 4. Rotating rod; 5. Bevel gear set; 6. Motor; 7. Protective cover; 8. Culture frame; 9. Placement tank; 10. Collection tank; 11. Control box; 12. Water storage frame; 13. Water inlet; 14. Water pipe; 15. Nozzle; 16. Lighting lamp; 17. Connecting block; 18. Connecting rod; 19. Sealing door. Detailed Implementation The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0014] Please see Figure 1-5 This utility model provides a technical solution: a plant tissue culture device, including a culture box 1. A base plate 2 is fixedly connected to the lower end of the culture box 1, and a fixing frame 3 is fixedly connected to the middle of the upper end of the base plate 2. A rotating rod 4 is arranged in the middle of the interior of the fixing frame 3. A bevel gear set 5 is sleeved on the outer side of the rotating rod 4, and the right side of the bevel gear set 5 is connected to the rotating shaft of a motor 6. A protective cover 7 is provided on the outer side of the motor 6. A culture frame 8 is fixedly connected to the upper end of the rotating rod 4, and placement grooves 9 are evenly distributed on the culture frame 8. A collection groove 10 is formed at the lower end of the interior of the culture frame 8. A control device is fixedly installed on the lower left side of the culture box 1. The upper end of the incubator 11 is fixedly connected to a water storage frame 12, and a water inlet 13 is opened on the upper left side of the water storage frame 12. Water pipes 14 are fixedly connected to both ends of the water storage frame 12, and the other end of the water pipes 14 is fixedly connected to a nozzle 15. The nozzle 15 is fixedly installed in the middle of the left and right sides inside the incubator 1. A light 16 is provided at the upper end of the inside of the incubator 1, and connecting blocks 17 are fixedly connected to both sides of the light 16. A connecting rod 18 is fixedly connected to the other end of the connecting block 17, and the connecting rod 18 is inserted into the left and right ends of the incubator 1. The left and right sides of the front surface of the incubator 1 are hinged to a closed door 19.

[0015] The bottom plate 2 has evenly spaced openings. The lower end of the incubator 1 has a sloping structure. A rubber sealing plug is inserted in the middle of the lower right side of the incubator 1. The incubator 1 has a matching slot. The front surface of the closed door 19 has a transparent observation window. The transparent observation window is made of high-transparency sterile glass, which is convenient for observation and can ensure airtightness.

[0016] The lower end of the rotating rod 4 is rotatably connected to the inner wall of the fixed frame 3. A bevel gear set 5 is provided on the rotating rod 4. The bevel gear set 5 consists of two sets of bevel gears. One set of bevel gears is sleeved on the rotating rod 4, and the other set of bevel gears is sleeved on the rotating shaft of the motor 6. The two sets of bevel gears mesh perpendicularly with each other to ensure the stability and accuracy of power transmission. The lower end of the protective cover 7 is fixedly installed on the fixed frame 3. Heat dissipation holes are provided on both the front and rear sides of the protective cover 7 to ensure heat dissipation while preventing dust from entering.

[0017] The culture frame 8 has evenly spaced placement grooves 9 on its upper ring. Rubber protrusions are fixedly connected to the inner wall of the placement grooves 9. The rubber protrusions are made of medical-grade rubber with good elasticity, which can clamp the culture container without damaging it. The lower end of the placement grooves 9 has evenly spaced openings with a diameter of 2-3mm, which can allow excess water to flow smoothly into the collection tank 10.

[0018] The lower end of the collection tank 10 is designed with a sloping structure with an inclination angle of 15-20° to facilitate the collection of water towards the drain outlet. A rubber sealing plug is inserted into the lower right side of the culture frame 8. A matching slot is provided on the culture frame 8, and the slot is connected to the collection tank 10. The rubber sealing plug fits tightly with the slot to ensure the sealing of the collection tank 10.

[0019] A rubber sleeve is fitted on the outer side of the upper end of the water inlet 13. The rubber sleeve is made of food-grade waterproof rubber to enhance the sealing of the water inlet 13. The lower end of the interior of the water storage frame 12 is set with an inverted V-shaped structure with an included angle of 60-90° to ensure that water flows completely into the water pipe 14. The upper end of the water pipe 14 is set with an inclined structure, and the lower end of the water pipe 14 is set with an opposing inclined structure. The spray nozzle 15 is set with an inclined structure with an angle of 30-45°, which can make the spray range cover the entire culture frame 8.

[0020] The connecting block 17 is configured in a T-shape and is movably engaged with the incubator 1. T-shaped grooves are provided on both the left and right sides of the incubator 1 to cooperate with it. The cooperation between the T-shape structure and the grooves ensures that the connecting block 17 does not shift when it moves. The connecting rod 18 is configured in a transverse U-shape. The incubator 1 has a groove that cooperates with the connecting rod 18. A locking bolt is inserted into the connecting rod 18 on the right side. The locking bolt is made of stainless steel, which is strong and not easy to rust. Locking slots that cooperate with the locking bolts are evenly provided on the incubator 1. The spacing between the locking slots is 5cm, which can realize the fine adjustment of the height of the lighting lamp 16.

[0021] Working principle When using this plant tissue culture device, first open the sealing door 19 and place the culture container containing the plant tissue into the placement slot 9 on the culture frame 8. The rubber convex balls on the inner wall of the placement slot 9 will clamp and fix the culture container to prevent shaking during subsequent rotation. Close the sealing door 19 and inject an appropriate amount of water or nutrient solution into the water storage frame 12 through the water inlet 13. The inverted V-shaped structure inside the water storage frame 12 will guide the water to the water pipes 14 at both ends, and then spray it evenly onto the plant tissue in the culture container through the nozzle 15 to achieve precise water replenishment. Excess water will flow into the collection tank 10 for storage through the opening at the bottom of the placement slot 9.

[0022] Adjust the height of the lighting lamp 16 according to the growth stage of the plant tissue: Pull out the locking bolt on the right connecting rod 18, push the connecting rod 18 to move the connecting block 17 up and down along the T-shaped sliding grooves on both sides of the culture box 1, thereby adjusting the height of the lighting lamp 16. When the height is appropriate, insert the locking bolt into the corresponding locking groove to fix the height of the lighting lamp 16. Turn on the lighting lamp 16 through the control box 11 to provide light for the growth of the plant tissue. At the same time, turn on the motor 6. The rotating shaft of the motor 6 drives the rotating rod 4 to rotate through the bevel gear set 5. The rotating rod 4 then drives the culture frame 8 to rotate at a uniform speed, so that the plant tissue in each placement groove 9 can receive light evenly and avoid uneven light exposure from affecting growth.

[0023] During the cultivation process, the growth of plant tissues can be observed at any time through the transparent observation window on the sealed door 19 without opening the device, ensuring a sterile environment inside the incubator 1. If there is excessive water accumulation in the collection tank 10, the rubber seal at the lower right end of the cultivation frame 8 can be pulled out to drain the water and then reinserted. If water accidentally drips from the bottom of the incubator 1, it can be drained through the rubber seal at the lower right end. The heat generated by the motor 6 during operation is dissipated through the heat dissipation holes on the front and rear sides of the protective cover 7, preventing the motor 6 from being damaged by overheating. The speed of the motor 6 can be adjusted through the control box 11 to adapt to the light uniformity requirements of different plant tissues, and the brightness of the lighting lamp 16 can also be adjusted to meet the light intensity requirements of different growth stages of plant tissues. Throughout the cultivation process, the device achieves functions such as uniform light reception, precise water replenishment, adjustable height, and convenient observation, effectively improving the efficiency and quality of plant tissue culture.

[0024] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended features rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the features be included within this invention. The scope of this invention is defined by its equivalents.

Claims

1. A plant tissue culture device, comprising an incubator (1), characterized in that: The lower end of the incubator (1) is fixedly connected to a base plate (2), and a fixed frame (3) is fixedly connected to the middle of the upper end of the base plate (2). A rotating rod (4) is set in the middle of the inside of the fixed frame (3). A bevel gear set (5) is sleeved on the outside of the rotating rod (4), and the right side of the bevel gear set (5) is connected to the rotating shaft of the motor (6). A protective cover (7) is set on the outside of the motor (6). A culture frame (8) is fixedly connected to the upper end of the rotating rod (4), and a placement groove (9) is evenly opened on the culture frame (8). A collection groove (10) is opened at the lower end of the inside of the culture frame (8). A control box (11) is fixedly installed at the lower left side of the incubator (1). The upper end of the incubator (1) is fixedly connected to the base plate (2). A water storage frame (12) is fixedly connected, and a water inlet (13) is opened on the upper left side of the water storage frame (12). Water pipes (14) are fixedly connected to both the left and right ends of the water storage frame (12), and the other end of the water pipes (14) is fixedly connected to the nozzle (15). The nozzle (15) is fixedly installed in the middle of the left and right sides inside the incubator (1). A lighting lamp (16) is provided at the upper end of the incubator (1), and connecting blocks (17) are fixedly connected to both the left and right sides of the lighting lamp (16). A connecting rod (18) is fixedly connected to the other end of the connecting block (17), and the connecting rod (18) is inserted into the left and right ends of the incubator (1). A closed door (19) is hinged to both the left and right sides of the front surface of the incubator (1).

2. The plant tissue culture device according to claim 1, characterized in that: The bottom plate (2) has openings evenly distributed. The lower end of the incubator (1) is provided with a sloping structure. A rubber sealing plug is inserted in the middle of the lower right side of the incubator (1). The incubator (1) has a matching slot. The front surface of the closed door (19) is provided with a transparent observation window.

3. The plant tissue culture device according to claim 1, characterized in that: The lower end of the rotating rod (4) is rotatably connected to the inner wall of the fixed frame (3). A bevel gear set (5) is provided on the rotating rod (4). The bevel gear set (5) consists of two sets of bevel gears. One set of bevel gears is sleeved on the rotating rod (4), and the other set of bevel gears is sleeved on the rotating shaft of the motor (6). The two sets of bevel gears mesh vertically with each other. The lower end of the protective cover (7) is fixedly installed on the fixed frame (3). Heat dissipation holes are provided on both the front and rear sides of the protective cover (7).

4. The plant tissue culture device according to claim 1, characterized in that: The culture frame (8) is evenly provided with placement grooves (9) on the ring. Rubber convex balls are fixedly connected to the inner wall of the placement grooves (9). The lower end of the placement grooves (9) is evenly provided with openings.

5. The plant tissue culture device according to claim 1, characterized in that: The lower end of the collection tank (10) is provided with a sloping structure. A rubber sealing plug is inserted into the lower right side of the culture frame (8). A slot is provided on the culture frame (8) to match it, and the slot is connected to the collection tank (10).

6. The plant tissue culture device according to claim 1, characterized in that: The upper outer side of the inlet (13) is fitted with a rubber sleeve, the lower inner end of the water storage frame (12) is provided with an inverted V-shaped structure, the upper end of the water pipe (14) is provided with an oblique structure, the lower end of the water pipe (14) is provided with an opposing oblique structure, and the spray nozzle (15) is provided with an oblique structure.

7. The plant tissue culture device according to claim 1, characterized in that: The connecting block (17) is configured in a T-shape and is movably engaged with the incubator (1). The incubator (1) has T-shaped grooves on both the left and right sides that cooperate with it. The connecting rod (18) is configured in a transverse U-shape. The incubator (1) has grooves that cooperate with the connecting rod (18). A locking bolt is inserted into the connecting rod (18) on the right side. The incubator (1) has evenly spaced locking grooves that cooperate with the locking bolts.

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