A microfluidic chip for blood testing

Abstract

The utility model provides a micro -fluidic chip for blood detection. The micro -fluidic chip for blood detection is disc -shaped and comprises a chip body (101), an elastic film (102) and a programmable module (103) from top to bottom; the chip body (101) is provided with a sample part, a reagent part, a reaction part, a washing liquid part, a waste liquid part and an annular channel (8); the annular channel (8) takes the center of the micro -fluidic chip for blood detection as the center; the sample part, the reagent part and the washing liquid part are located on the inner side of the annular channel, the reaction part is located on the outer side of the annular channel (8), and the waste liquid part is located on the outer side of the reaction part; the programmable module (103) can rotate and is provided with a valve control part on the upper surface to control the corresponding valves of the sample part, the reagent part, the reaction part, the washing liquid part and the waste liquid part. The micro -fluidic chip for blood detection only needs 0.3ml of whole blood to correspond to 60 detections, and has a lower sample requirement.

Description

Technical Field

[0001] This utility model relates to a microfluidic chip for blood testing, belonging to the field of blood sample testing technology. Background Technology

[0002] Currently, common allergens in clinical laboratory samples are detected using the immunoblotting method. The immunoblotting method typically detects no more than 42 allergen IgE antibodies at a time. This method involves coating allergens, control lines, and threshold lines parallel to each other on a nitrocellulose membrane. With one sample of serum or plasma and one test reaction, IgE antibodies against 42 allergens in human serum can be detected.

[0003] Furthermore, the detection chips currently used in testing are relatively simple and cannot simultaneously separate and detect blood samples within the chip. For example, CN216024952U discloses a microfluidic chip for blood detection. This microfluidic chip has a threaded rod vertically fixed at the top center of its base. A mounting plate is located at the top of the threaded rod, and several rotating plates are hinged to the outer circumference of the mounting plate. Chips are fixedly mounted on the outer side of the rotating plates. An adjusting block, a movable block, and a spring are sequentially fitted onto the outer circumference of the threaded rod from bottom to top. Several connecting rods are hinged to the outer circumference of the movable block, with the end of each connecting rod furthest from the movable block rotatably connected to the bottom of the rotating plate. By using the adjusting block, the movable block can be raised and lowered by rotating the adjusting block, which in turn causes the rotating plates to swing via the connecting rods, thereby adjusting the working angle of multiple chips and ensuring rapid detection of blood with high lipid levels. However, this microfluidic chip is only suitable for detecting the flow rate of blood with high lipid levels and cannot separate and detect cells in blood samples.

[0004] Therefore, developing a multifunctional microfluidic chip for blood testing is a technical problem that urgently needs to be solved in this field. Utility Model Content

[0005] To address the aforementioned technical problems, the purpose of this utility model is to provide a microfluidic chip that can integrate and programmatically control sample components, reagent components, and washing solution components.

[0006] To achieve the above objectives, this utility model provides a microfluidic chip for blood detection, wherein the microfluidic chip for blood detection is disc-shaped and includes, from top to bottom, a chip body, an elastic membrane, and a programmable module;

[0007] The chip body is provided with a sample section, a reagent section, a reaction section, a washing solution section, a waste solution section, and an annular channel; the annular channel is centered on the center of the microfluidic chip used for blood detection; the sample section, reagent section, and washing solution section are located inside the annular channel, the reaction section is located outside the annular channel, and the waste solution section is located outside the reaction section;

[0008] The sample section includes at least one combination of sample pools and sample channels, wherein the inlet of the sample channel is connected to the sample pool via an injection valve and the outlet is connected to the annular channel;

[0009] The reagent section includes at least one combination of reagent pool and reagent channel, wherein the inlet and outlet of the reagent channel are respectively connected to the reagent pool and the annular channel;

[0010] The reaction section includes 20-80 combinations of reaction channels, reaction tanks, and waste liquid channels, and the waste liquid section includes 20-80 waste liquid tanks; the inlet and outlet of the reaction channel are respectively connected to the annular channel and the reaction tank; the inlet and outlet of the waste liquid channel are respectively connected to the reaction tank and the waste liquid tank.

[0011] The washing solution section includes at least one combination of a washing solution tank and a washing solution channel; the inlet and outlet of the washing solution channel are respectively connected to the washing solution tank and the annular channel.

[0012] The sample channel is equipped with valves that control the corresponding channels, such as the sample injection valve between the sample channel and the annular channel, the reagent channel, the reaction channel, the waste liquid channel, and the washing liquid channel.

[0013] The programmable module is rotatable and has a valve control section on its upper surface that controls each valve.

[0014] According to a specific embodiment of the present invention, preferably, the chip body is a ring-shaped (i.e., a disk with a through hole in the center), and its upper and lower surfaces are respectively formed with corresponding structures, such as various pools and channels.

[0015] According to a specific embodiment of this utility model, preferably, the elastic film is a transparent elastic film. Using a transparent elastic film facilitates fluorescence detection. The elastic film is disposed on the lower surface of the chip body and is used to seal the pools, channels, etc., on the lower surface of the chip body. This elastic film can be made of commonly used elastic resin film, elastic plastic film, etc.

[0016] According to a specific embodiment of this utility model, preferably, the sample section, reagent section, and washing solution section are located inside the annular channel (i.e., the side closer to the center), the reaction section is located outside the annular channel (i.e., the side farther from the center), and the waste liquid section is located further outside the reaction section; the outlets of the sample section, reagent section, and washing solution section are respectively connected to the annular channel, the annular channel is connected to the inlet of the reaction section, and the outlet of the reaction section is connected to the inlet of the waste liquid section. This arrangement facilitates the sequential addition of samples, reagents, and washing solutions to the reaction section during centrifugation, and after the reaction, the waste liquid is discharged into the waste liquid section via centrifugation.

[0017] According to a specific embodiment of the present invention, preferably, the bottom surfaces of the annular channel, the reagent pool, the reaction pool, the washing liquid pool, the sample channel, the reagent channel, the reaction channel, the waste liquid channel, and the washing liquid channel are respectively located on the lower surface of the chip body and are sealed by the elastic membrane.

[0018] According to a specific embodiment of the present invention, preferably, the programmable module is a disc-shaped part with a hole in the center, including a central part and an outer peripheral part. The two parts are not independent of each other, but are two parts of the programmable module divided by the fluorescence observation hole.

[0019] According to a specific embodiment of this utility model, preferably, the microfluidic chip for blood detection further includes a chip base; the chip base is located at the bottom of the center position of the microfluidic chip for blood detection. The chip base can be connected by means of threads, adhesive bonding, or bonding. Preferably, the edge of the chip base has external threads and the inner wall of the central hole of the programmable module has internal threads, and the two are mutually adapted.

[0020] According to a specific embodiment of this utility model, preferably, the sample pool includes a top sample pool compartment and a bottom sample pool compartment that are interconnected. The top sample pool compartment is a through hole located at the center of the chip body, and the bottom sample pool compartment is located inside the chip base. Preferably, the bottom sample pool compartment has a protrusion at its center, which is a frustum-shaped cone with an isosceles trapezoidal longitudinal section.

[0021] According to a specific embodiment of this utility model, preferably, the horizontal position of the entrance of the sample channel is located at the horizontal dividing line between the top compartment and the bottom compartment of the sample pool.

[0022] According to a specific embodiment of this utility model, preferably, the volume of the bottom compartment of the sample pool accounts for more than 55% of the total volume of the top compartment and the bottom compartment of the sample pool, more preferably more than 60%. Generally, the volume of red blood cells in human whole blood is less than 50%. By controlling the proportion of the bottom compartment's volume within the above range, it can be ensured that after blood sample deposition, the horizontal position of the sample channel entrance is higher than that of red blood cells, ensuring that only serum flows into the sample channel.

[0023] According to a specific embodiment of this utility model, preferably, the injection valve is a water-soluble valve located at the inlet of the sample channel. The water-soluble valve can be made of a soluble adhesive, such as modified polypropylene resin or a hydrolyzable UV adhesive. The modified polypropylene resin is an inert material and will not affect sample detection after dissolution. The hydrolyzable UV adhesive can be a commonly used adhesive in the art; water or other liquids can dissolve and desorb the hydrolyzable UV adhesive. In use, the following steps can be taken: apply the hydrolyzable UV adhesive to the desired location (i.e., the inlet of the sample channel), and then perform UV curing to form the water-soluble valve; after adding a liquid sample to the chip, the cured adhesive will dissolve and desorb, thereby opening the valve. The dissolution time of the water-soluble valve (i.e., the time from contact with water to complete dissolution) can be controlled by the amount of water-soluble adhesive and the UV curing time. That is, the larger the amount of water-soluble adhesive and the longer the UV curing time, the longer the dissolution time of the water-soluble adhesive. Specifically, it can be selected and controlled according to the required dissolution time. Under normal circumstances, the dissolution time can be controlled so that the water-soluble valve is completely dissolved after the whole blood sample is centrifuged.

[0024] During centrifugation, blood cells in the blood sample in the sample pool will be deposited in the bottom chamber of the sample pool under the action of centrifugal force, while plasma can remain on the upper layer of blood cells. After the injection valve is opened (e.g., to dissolve), plasma can enter the sample channel under the action of centrifugal force, while blood cells will remain in the bottom chamber of the sample pool, thus achieving the separation of plasma and blood cells.

[0025] According to a specific embodiment of the present invention, preferably, the valve includes an upper and lower corresponding valve ball and a groove, wherein the valve ball can be pushed up into the groove to close the corresponding channel;

[0026] The valve control unit is located on the upper surface of the programmable module, corresponding to the position of the corresponding valve. The valve control unit includes several points, each point being either a protrusion or a space. The protrusion is a raised structure on the surface of the programmable module, and the space is a structure flush with the surface of the programmable module; wherein:

[0027] When the protrusion is located below the corresponding valve, the protrusion lifts the corresponding valve ball, and the corresponding valve ball enters the corresponding groove, thereby closing the corresponding channel.

[0028] When the empty space is located below the corresponding valve, the empty space does not lift the corresponding valve ball, and the corresponding valve ball does not enter the groove, thereby keeping the corresponding channel in the open state.

[0029] According to a specific embodiment of this utility model, preferably, in the valve control section, the arrangement of protrusions and vacancy has a certain curvature to adapt to the overall shape of the chip and better correspond to the position of the valve ball.

[0030] According to a specific embodiment of this utility model, in the initial state, the first position of the valve control unit is located below the valve ball of the corresponding valve; during use, according to the usage program, the programmable module is rotated once for each step, and the valve control unit advances one position, thereby controlling the opening or closing of each valve.

[0031] According to a specific embodiment of the present invention, preferably, the protrusion is a cylindrical protrusion or other suitable shape.

[0032] According to a specific embodiment of the present invention, preferably, in the valve control section, when there are multiple protrusions in succession, these protrusions can be connected together to form a whole convex ridge or convex strip.

[0033] According to a specific embodiment of the present invention, preferably, the empty space being a structure flush with the surface of the programmable module means that there is no protruding structure at the corresponding position.

[0034] According to a specific embodiment of this utility model, preferably, the valve includes a sample valve, a reagent valve, a reaction valve, a discharge valve, and a washing liquid valve, which are respectively used to control the sample channel, the reagent channel, the reaction channel, the waste liquid channel, and the washing liquid channel.

[0035] According to a specific embodiment of the present invention, preferably, the sample valve includes a sample valve ball and a sample valve groove that are positioned vertically. The sample valve ball is located in a slot at the bottom of the sample channel, and the sample valve groove is located at the top of the sample channel. It is an upward-facing groove of the chip body at the corresponding position. The size of the sample valve ball is larger than the size of the sample channel. When the sample valve ball is pushed up, it enters the sample valve groove, thereby closing the sample channel.

[0036] The valve control unit includes a sample valve control unit corresponding to the sample valve. The sample valve control unit is used to control the opening and closing of the sample valve. The sample valve control unit includes 8 points, which are, in sequence, a raised position, an empty position, a raised position, a raised position, a raised position, a raised position, a raised position, a raised position, and a raised position.

[0037] According to a specific embodiment of the present invention, preferably, the reagent valve includes a reagent valve ball and a reagent valve groove that are positioned vertically. The reagent valve ball is located in a slot at the bottom of the reagent channel, and the reagent valve groove is located at the top of the reagent channel. It is an upward-facing groove of the chip body at the corresponding position. The size of the reagent valve ball is larger than the size of the reagent channel. When the reagent valve ball is pushed up, it enters the reagent valve groove, thereby closing the reagent channel.

[0038] The valve control unit includes a reagent valve control unit corresponding to the reagent valve. The reagent valve control unit is used to control the opening and closing of the reagent valve. The reagent valve control unit includes 8 points, which are, in sequence, protrusion, protrusion, protrusion, empty, protrusion, protrusion, and protrusion.

[0039] According to a specific embodiment of the present invention, preferably, the reaction valve includes a reaction valve ball and a reaction valve groove that are positioned vertically. The reaction valve ball is located in a slot at the bottom of the reaction channel, and the reaction valve groove is located at the top of the reaction channel. It is an upward-facing groove of the chip body at the corresponding position. The size of the reaction valve ball is larger than the size of the reaction channel. When the reaction valve ball is lifted, it enters the reaction valve groove, thereby closing the reaction channel.

[0040] The valve control unit includes a reaction valve control unit corresponding to the reaction valve. The reaction valve control unit is used to control the opening and closing of the reaction valve. The reaction valve control unit includes 8 points, which are, in order, protrusion, empty, protrusion, empty, empty, protrusion, empty, and protrusion.

[0041] According to a specific embodiment of this utility model, preferably, the discharge valve includes a discharge valve ball and a discharge valve groove that are positioned vertically. The discharge valve ball is located in a slot at the bottom of the waste liquid channel, and the discharge valve groove is located at the top of the waste liquid channel. It is an upward-facing groove of the chip body at the corresponding position. The size of the discharge valve ball is larger than the size of the waste liquid channel. When the discharge valve ball is lifted, it enters the discharge valve groove, thereby closing the waste liquid channel.

[0042] The valve control unit includes a discharge valve control unit corresponding to the discharge valve. The discharge valve control unit is used to control the opening and closing of the discharge valve. The discharge valve control unit includes 8 points, which are, in order, protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, and protrusion.

[0043] According to a specific embodiment of the present invention, preferably, the washing valve includes a washing valve ball and a washing valve groove that are positioned vertically. The washing valve ball is located in a slot at the bottom of the washing channel, and the washing valve groove is located at the top of the washing channel. It is an upward-facing groove of the chip body at the corresponding position. The size of the washing valve ball is larger than the size of the washing channel. When the washing valve ball is pushed up, it enters the washing valve groove, thereby closing the washing channel.

[0044] The valve control unit includes a washing liquid valve control unit corresponding to the washing liquid valve. The washing liquid valve control unit is used to control the opening and closing of the washing liquid valve. The washing liquid valve control unit includes 8 points, which are, in order, protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, and protrusion.

[0045] According to a specific embodiment of the present invention, preferably, the sample channel, the reagent channel, the reaction channel, the waste liquid channel, and the washing liquid channel are arranged along the radial direction of the microfluidic chip used for blood detection.

[0046] According to a specific embodiment of this utility model, preferably, the combination of 20-80 sets of reaction channels, reaction pools, and waste liquid channels, as well as the 20-80 waste liquid pools, are evenly distributed along the circumference of the microfluidic chip used for blood detection. More preferably, the number of reaction channels is 50-70 sets, the number of waste liquid pools is 50-70, and the number of reaction channels is the same as the number of waste liquid pools.

[0047] According to a specific embodiment of the present invention, preferably, adjacent waste liquid tanks are separated by a partition.

[0048] According to a specific embodiment of this utility model, preferably, the reaction tank is provided with magnetic microspheres. More preferably, the magnetic microspheres are magnetic microspheres labeled with different allergens. The number of magnetic microspheres can be selected as needed.

[0049] According to a specific embodiment of the present invention, preferably, the reagent pool contains a pre-set antibody, such as a fluorescently labeled mouse anti-human IgE antibody.

[0050] According to a specific embodiment of the present invention, preferably, the washing solution tank is pre-filled with washing solution, such as the washing solution commonly used in blood sample testing.

[0051] According to a specific embodiment of the present invention, preferably, the programmable module is provided with a fluorescence observation hole at the position corresponding to the reaction cell.

[0052] According to a specific embodiment of the present invention, preferably, the microfluidic chip for blood detection further includes a positioning track, which is located between the elastic membrane and the programmable module;

[0053] The positioning track has five tracks, corresponding to the sample valve control unit, reagent valve control unit, washing liquid valve control unit, reaction valve control unit, and discharge valve control unit, so that it can rotate along the track.

[0054] According to a specific embodiment of the present invention, preferably, the microfluidic chip for blood detection has a five-layer structure, wherein the chip body is the first layer, the elastic membrane is the second layer, the positioning track is the third layer, the programmable module is the fourth layer, and the chip base is the fifth layer.

[0055] According to a specific embodiment of this utility model, preferably, the microfluidic chip for blood detection is provided with positioning buckles and rotating buckles on its outer periphery, and the rotating buckles are connected to the programmable module. The positioning buckles are used to connect with buckles on the chip holder of an external fluorescence detection device to fix the microfluidic chip for fluorescence detection; the rotating buckles are used to connect with the valve control motor of an external centrifuge device so that, driven by the valve control motor, the programmable module can be rotated according to the detection program, thereby controlling the programmable module to rotate by a certain amplitude, thus controlling the opening and closing of each valve. The two positioning buckles and two rotating buckles can be arranged around the outer periphery of the microfluidic chip for blood detection. The specific positions of the positioning buckles and rotating buckles on the outer circumference can be selected as needed, as long as they do not overlap or affect their respective functions.

[0056] The microfluidic chip for blood testing provided by this invention can be made of materials commonly used in the field, such as polymethacrylate, polystyrene, cyclic olefin copolymers, polycarbonate, etc., and can be prepared by conventional methods such as injection molding and cutting.

[0057] The microfluidic chip for blood testing provided by this invention has the following advantages:

[0058] 1. Small sample requirement: Existing blood testing methods generally require 2 mL of serum (about 4-5 mL of whole blood) for testing, while the microfluidic chip for blood testing of this invention generally only requires 0.3 mL of whole blood (corresponding to 60 tests).

[0059] 2. Automatic operation: The microfluidic chip for blood testing of this invention is pre-loaded with various reagents and washing solutions, enabling automatic operation.

[0060] 3. Short testing time: Existing blood testing methods generally require 2.5-3 hours, while the microfluidic chip for blood testing of this invention generally only requires 20-30 minutes for testing.

[0061] 4. Multiple detection items: Existing blood testing methods can detect a maximum of 48 items per sample addition, while the microfluidic chip for blood testing of this invention can preferably achieve more than 60 detection items, and the number of detection items can be flexibly adjusted according to needs.

[0062] 5. High sensitivity: Existing blood testing methods generally rely on visual inspection or scanners for interpretation, which can only perform qualitative or semi-quantitative detection and has relatively low sensitivity. The microfluidic chip for blood testing of this invention can be interpreted with the aid of a fluorescence reader, enabling quantitative detection and achieving high sensitivity. Attached Figure Description

[0063] Figure 1 This is a top view of the microfluidic chip for blood detection provided in Example 1.

[0064] Figure 2 for Figure 1 A magnified view of a local area.

[0065] Figure 3 for Figure 1 A cross-sectional view along direction AA.

[0066] Figure 4 for Figure 3 A magnified view of a local area.

[0067] Figure 5 for Figure 1 The BB-direction cross-sectional view.

[0068] Figure 6 for Figure 5 A magnified view of a local area.

[0069] Figure 7 for Figure 1 A cross-sectional view along the CC direction.

[0070] Figure 8 for Figure 7 A magnified view of a local area.

[0071] Figure 9 for Figure 1 The DD-direction cross-sectional view.

[0072] Figure 10 for Figure 9 A magnified view of a local area.

[0073] Figure 11This is a top view of the programmable module.

[0074] Figure 12 This is a schematic diagram showing the location of the valve control unit in a programmable module.

[0075] Figure 13 for Figure 12 A magnified view of a local area.

[0076] Figure 14 for Figure 13 A magnified view of a local area.

[0077] Figure 15 This is a schematic diagram of the chip base and sample valve.

[0078] Explanation of main icon numbers:

[0079] Chip body 101, elastic membrane 102, programmable module 103, chip base 104, positioning track 105, center part 106, outer peripheral part 107, fluorescence observation hole 108, sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and sample discharge valve control unit 1035;

[0080] Sample pool 201, sample injection valve 202, sample channel 203, sample valve 204, sample pool wall 205, sample pool top compartment 206, sample pool bottom compartment 207, sample valve ball 208, sample valve groove 209;

[0081] Reagent pool 301, reagent channel 302, reagent valve 303, reagent valve ball 304, reagent valve groove 305, reagent pool inlet 306;

[0082] Reaction channel 401, reaction valve 402, reaction tank 403, waste liquid channel 404, discharge valve 405, reaction valve ball 406, reaction valve groove 407, discharge valve ball 408, discharge valve groove 409;

[0083] 501 Washing solution tank, 502 Washing solution channel, 503 Washing solution valve, 504 Washing solution valve ball, 505 Washing solution valve groove, 506 Washing solution tank inlet;

[0084] Waste liquid tank 601, baffle 602;

[0085] Positioning buckle 701, rotating buckle 702;

[0086] Circular channel 8. Detailed Implementation

[0087] In order to provide a clearer understanding of the technical features, objectives and beneficial effects of this utility model, the technical solution of this utility model is described in detail below, but it should not be construed as limiting the scope of implementation of this utility model.

[0088] Terminology Explanation:

[0089] Proximal end and distal end: In this utility model, the proximal end refers to the end that is relatively close to the center of the microfluidic chip, and the distal end refers to the end that is relatively far away from the center of the microfluidic chip.

[0090] Valve (valve) open state and closed state: In this utility model, the valve open state refers to the state in which the channel controlled by the valve is kept open, and the valve closed state refers to the state in which the channel controlled by the valve is kept closed. The specific control and switching can be realized by a programmable module.

[0091] Example 1

[0092] This embodiment provides a microfluidic chip for blood detection, the structure of which is as follows: Figures 1-10 As shown.

[0093] The structure of the microfluidic chip in this embodiment will be described in detail below with reference to the accompanying drawings:

[0094] like Figure 1 and Figure 3 As shown, the microfluidic chip is generally disk-shaped and has a five-layer structure, including a chip body 101, an elastic membrane 102, a positioning track 105, a programmable module 103, and a chip base 104. The chip body 101 is the first layer, the elastic membrane 102 is the second layer, the positioning track 105 is the third layer, the programmable module 103 is the fourth layer, and the chip base 104 is the fifth layer.

[0095] The microfluidic chip also has two positioning latches 701 and two rotating latches 702. The two positioning latches 701 and the two rotating latches 702 are arranged around the outer perimeter of the microfluidic chip. The two positioning latches 701 are arranged opposite each other, and the two rotating latches 702 are arranged opposite each other (the positions of the positioning latches 701 and the rotating latches 702 are not limited to this, as long as they do not overlap and do not affect their respective functions).

[0096] The positioning buckle 701 is used to connect with the buckle on the chip holder of the external fluorescence detection device to fix the microfluidic chip for centrifugation.

[0097] The rotating buckle 702 is used to connect to the valve control motor of the external centrifugal device so that the rotation control of the programmable module 103 is realized according to the detection program under the drive of the valve control motor, thereby controlling the programmable module to rotate at a certain amplitude, thereby controlling the opening and closing of each valve;

[0098] in:

[0099] The chip body 101 is generally in the shape of a ring, and its upper and lower surfaces have corresponding structures, such as various cells and channels.

[0100] The elastic film 102 is a transparent elastic film, located on the lower surface of the chip body 101, specifically between the chip body 101 and the positioning track 105 and the programmable module 103. Figure 3 As shown; the elastic membrane 102 is used to seal the pools, channels, etc. on the lower surface of the chip body 101;

[0101] Programmable module 103 is generally a disc with a hole in the center (e.g.) Figure 11 As shown), the programmable module 103 includes a central portion 106 and an outer peripheral portion 107. These two portions are not independent but rather represent two regions divided by the fluorescence observation hole 108. The programmable module 103 is located below the chip body 101, and its edge is connected to a rotating latch 702. By rotating the rotating latch 702, the programmable module 103 can be rotated around the chip body 101, thereby controlling the corresponding valves. The programmable module 103 has corresponding valve control sections (such as...) at the positions of each valve. Figure 12 (as shown)

[0102] The chip base 104 has a circular cross-section and is located at the bottom of the center of the microfluidic chip. The edge of the chip base 104 is provided with external threads and the inner wall of the central hole of the programmable module 103 is provided with internal threads, and the two are compatible with each other.

[0103] The chip body 101 is provided with a sample section, a reagent section, a reaction section, a washing solution section, a waste solution section, and an annular channel 8. The annular channel 8 is a circular channel located on the lower surface of the chip body 101, with a square cross-section centered on the center of the chip body 101. The sample section, reagent section, and washing solution section are located inside the annular channel 8 (i.e., the side closer to the center), the reaction section is located outside the annular channel 8 (i.e., the side farther from the center), and the waste solution section is located even further outside the reaction section. The outlets of the sample section, reagent section, and washing solution section are respectively connected to the annular channel 8, the annular channel 8 is connected to the inlet of the reaction section, and the outlet of the reaction section is connected to the inlet of the waste solution section.

[0104] like Figures 1-4As shown, the sample unit includes a sample pool 201, a sample injection valve 202, a sample channel 203, and a sample valve 204;

[0105] Sample pool 201 includes a top sample pool compartment 206 and a bottom sample pool compartment 207 that are interconnected. The top sample pool compartment 206 is located at the center of the chip body 101 and is a circular through-hole formed by the chip body 101, with its sidewalls forming the sample pool walls 205. The bottom sample pool compartment 207 is located inside the chip base 104 (e.g., ...). Figure 15 As shown); the center of the bottom compartment 207 of the sample pool has a protrusion, which is a frustum-shaped cone with an isosceles trapezoidal longitudinal section and a base angle of 45°.

[0106] The sample channel 203 is disposed on the bottom surface of the chip body 101 along the radial direction of the chip body 101, and its cross-section is square; furthermore, the proximal end (inlet) of the sample channel 203 is connected to the sample cell 201, and the distal end (outlet) is connected to the annular channel 8; a sample injection valve 202 is provided at the inlet of the sample channel 203 that connects to the sample cell 201 (e.g., Figure 15 As shown), the injection valve 202 is a water-soluble valve. Before use, the injection valve 202 is in the closed state, that is, the sample channel 203 is closed.

[0107] A sample valve 204 is provided near the outlet of the sample channel 203 that connects to the annular channel 8. The sample valve 204 includes a sample valve ball 208 and a sample valve groove 209 that are positioned vertically. The sample valve ball 208 is located in the groove at the bottom of the sample channel 203, specifically on the upper surface of the center 106 of the programmable module 103. The sample valve ball 208 is connected to the elastic membrane 102, that is, the upper and lower hemispheres of the sample valve ball 208 are located above and below the elastic membrane 102, respectively. Other valve balls are in a similar positional relationship with the elastic membrane 102. The sample valve groove 209 is located at the top of the sample channel 203. It is an upward groove of the chip body 101 at the corresponding position. The size of the sample valve ball 208 is larger than the size of the sample channel 203. When the sample valve ball 208 is lifted, it can (partially) enter the sample valve groove 209 to close the sample channel 203. Figure 4 The display shows the situation when the sample valve ball 208 is not pushed up, at which time the sample channel 203 is open; Figure 8 The display shows the situation where the discharge valve ball 408 is pushed up and enters the discharge valve groove 409 to close the waste liquid channel 404. The situation is the same when the sample valve ball 208 is pushed up.

[0108] A sample valve control unit 1031 is provided at a corresponding position in the center 106 of the programmable module 103 (e.g., Figures 11-12As shown), the sample valve control unit 1031 can control the opening and closing of the sample valve 204. It includes eight positions, which are, in sequence, protrusion, empty, protrusion, protrusion, protrusion, protrusion, and protrusion (as shown). Figure 13 , Figure 14 As shown, the solid black dots represent protrusions, i.e., the protruding structures on the surface of the programmable module 103; the hollow dashed dots represent empty spaces (meaning there are no protruding structures at that location, and the space is flush with the surface of the programmable module 103). When the programmable module 103 is rotated, the corresponding point of the sample valve control unit 1031 can be rotated to below the sample valve 204. Specifically: when the protrusion is rotated to below the sample valve 204, the protrusion lifts the sample valve ball 208, causing it to enter the sample valve groove 209, thereby closing the sample channel 203. When the empty space is rotated to below the sample valve 204, the empty space does not lift the sample valve ball 208, and the sample valve ball 208 does not enter the sample valve groove 209 (if the previous point is a protrusion, then the sample valve ball 208 falls from the sample valve groove 209), thereby opening the sample channel 203. Thus, the open and closed states of the sample channel 203 can be controlled according to the program.

[0109] like Figures 1-2 , Figures 5-6 As shown, the reagent section includes a reagent pool 301, a reagent channel 302, and a reagent valve 303;

[0110] The reagent pool 301 is located on the upper part of the chip body 101 (below the surface layer, and will not be exposed after assembly; an opening can be left at one end), and is fan-shaped. The near end of the reagent pool 301 is provided with a reagent pool inlet 306 for adding reagents. Its specific position and shape can be selected as needed. The reagent pool inlet 306 is provided with a corresponding sealing cap. The reagent pool 301 is pre-filled with fluorescently labeled mouse anti-human IgE antibody.

[0111] The reagent channel 302 is disposed on the bottom surface of the chip body 101 along the radial direction of the chip body 101, and its cross-section is square;

[0112] The reagent pool 301 is connected to the proximal end of the reagent channel 302, and the connection point between the two is located at the center of the arc at the distal end of the reagent pool 301; the distal end of the reagent channel 302 is connected to the annular channel 8.

[0113] A reagent valve 303 is provided on the reagent channel 302. The reagent valve 303 includes a reagent valve ball 304 and a reagent valve groove 305, which are located at corresponding positions. The reagent valve ball 304 is located in the groove at the bottom of the reagent channel 302, specifically on the upper surface of the center part 106 of the programmable module 103. The reagent valve groove 305 is located at the top of the reagent channel 302, which is the upward groove of the chip body 101 at the corresponding position. The size of the reagent valve ball 304 is larger than the size of the reagent channel 302. When the reagent valve ball 304 is raised, it can enter the reagent valve groove 305 to close the reagent channel 302.

[0114] A reagent valve control unit 1032 is provided at a corresponding position in the center 106 of the programmable module 103 (e.g., ...). Figure 12 , Figure 13 As shown), the reagent valve control unit 1032 can control the opening and closing of the reagent valve 303. It includes 8 points, in sequence: protrusion, protrusion, protrusion, protrusion, empty, protrusion, protrusion, protrusion (as shown). Figure 13 As shown, the reagent valve control unit 1032 operates in the same way as the sample valve control unit 1031, and will not be described again here;

[0115] like Figures 1-2 , Figures 7-8 As shown, the reaction section includes 60 reaction channels 401, 60 reaction valves 402, 60 reaction pools 403, 60 waste liquid channels 404, and 60 discharge valves 405; the waste liquid section includes 60 waste liquid pools 601, with adjacent waste liquid pools 601 separated by partitions 602; one reaction channel 401, one reaction valve 402, one reaction pool 403, one waste liquid channel, one discharge valve, and one waste liquid pool constitute a combination, and 60 such combinations are radiating outwards and evenly distributed along the outer edge (circumference of the microfluidic chip) of the chip body 101; magnetic microspheres are pre-installed in the reaction pools 403, and the magnetic microspheres in different reaction pools 403 are labeled with different allergens;

[0116] The reaction channel 401 and the waste liquid channel 404 are arranged on the bottom surface of the chip body 101 along the radial direction of the chip body 101, and their cross-sections are square.

[0117] The proximal end (inlet) of reaction channel 401 is connected to the annular channel 8, and the distal end (outlet) is connected to the inlet of reaction tank 403;

[0118] A reaction valve 402 is provided on the reaction channel 401. The reaction valve 402 includes a reaction valve ball 406 and a reaction valve groove 407 that are positioned vertically. The reaction valve ball 406 is located in the groove at the bottom of the reaction channel 401, specifically on the upper surface of the center 106 of the programmable module 103. The reaction valve groove 407 is located at the top of the reaction channel 401, which is an upward groove of the chip body 101 at the corresponding position. The size of the reaction valve ball 406 is larger than the size of the reaction channel 401. When the reaction valve ball 406 is raised, it can enter the reaction valve groove 407 to close the reaction channel 401.

[0119] Sixty reaction valve control units 1034 (e.g., ...) are provided at corresponding positions in the central part 106 of the programmable module 103. Figure 12 , Figure 13 As shown), each reaction valve control unit 1034 controls the opening and closing of a reaction valve 402, which includes 8 points, in the following order: protrusion, empty, protrusion, empty, empty, protrusion, empty, protrusion (as shown). Figure 14 As shown, the operation of the reaction valve control unit 1034 is the same as that of the sample valve control unit 1031, and will not be described again here;

[0120] The reaction cell 403 is disc-shaped and located on the bottom surface of the chip body 101, with its bottom sealed by an elastic membrane 102. The outlet of the reaction cell 403 is connected to the proximal inlet of the waste liquid channel 404, and the distal outlet of the waste liquid channel 404 is connected to the waste liquid pool 601 (e.g., Figure 8 (as shown)

[0121] The waste liquid channel 404 is provided with a discharge valve 405. The discharge valve 405 has a discharge valve ball 408 and a discharge valve groove 409. The discharge valve ball 408 is located in the groove at the bottom of the waste liquid channel 404, specifically on the upper surface of the outer periphery 107 of the programmable module 103. The discharge valve groove 409 is located at the top of the waste liquid channel 404, which is the upward groove of the chip body 101 at the corresponding position. The size of the discharge valve ball 408 is larger than the size of the waste liquid channel 404. When the discharge valve ball 408 is raised, it can enter the discharge valve groove 409 to close the waste liquid channel 404.

[0122] Sixty discharge valve control units 1035 (e.g., ...) are provided at corresponding positions on the outer periphery 107 of the programmable module 103. Figure 12 , Figure 13 As shown), each discharge valve control unit 1035 controls the opening and closing of a discharge valve 405, which includes 8 points, in the following order: protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, protrusion (as shown). Figure 14 As shown, the operation of the discharge valve control unit 1035 is the same as that of the sample valve control unit 1031, and will not be described again here;

[0123] like Figures 1-2 , Figures 9-10 As shown, the washing solution section includes one washing solution tank 501, four washing solution channels 502, and four washing solution valves 503; wherein, the washing solution tank 501 can be pre-filled with cleaning solution;

[0124] The washing solution pool 501 is located on the upper part of the chip body 101 (below the surface layer) and is fan-shaped. The washing solution pool 501 has a washing solution pool inlet 506 at its near end. The specific location and shape can be selected as needed. The washing solution pool inlet 506 is equipped with a corresponding sealing cover.

[0125] Four washing liquid channels 502 are arranged along the radial direction of the chip body 101 on the bottom surface of the chip body 101, and their cross-section is square.

[0126] The washing liquid tank 501 is connected to the proximal end (inlet) of each washing liquid channel 502, and the connection point is located at the arc at the far end of the washing liquid tank 501. The four washing liquid channels 502 are evenly distributed along the far edge of the washing liquid tank 501. The far ends (outlets) of the four washing liquid channels 502 are connected to the annular channel 8 respectively.

[0127] Each washing liquid channel 502 is provided with a washing liquid valve 503. The washing liquid valve 503 includes a washing liquid valve ball 504 and a washing liquid valve groove 505, which are located at corresponding positions. The washing liquid valve ball 504 is located in the groove at the bottom of the washing liquid channel 502, specifically on the upper surface of the center part 106 of the programmable module 103. The washing liquid valve groove 505 is located at the top of the washing liquid channel 502, which is the upward groove of the chip body 101 at the corresponding position. The size of the washing liquid valve ball 504 is larger than the size of the washing liquid channel 502. When the washing liquid valve ball 504 is raised, it can enter the washing liquid valve groove 505 to close the washing liquid channel 502.

[0128] Four washing liquid valve control units 1033 (e.g., in the corresponding positions of the central part 106 of the programmable module 103) are provided. Figure 12 , Figure 13 As shown), each washing liquid valve control unit 1033 controls the opening and closing of a washing liquid valve 503, which includes 8 points, in the following order: protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, protrusion (as shown). Figure 14 As shown in the figure, the function of the washing liquid valve control unit 1033 is the same as that of the sample valve control unit 1031, and will not be described again here;

[0129] The positioning track 105 has five tracks, which are respectively used to accommodate the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and sample discharge valve control unit 1035, so that they can rotate along the tracks (e.g., Figure 4 , Figure 6 , Figure 8 , Figure 10 (as shown)

[0130] The specific location of the elastic membrane 102 corresponds to the areas of the reagent channel 302, reaction channel 401, reaction tank 403, waste liquid channel 404, etc., and is used for sealing. The elastic membrane 102 can be circular in shape.

[0131] When using the microfluidic chip of Example 1 to test blood samples, the following steps can be followed:

[0132] Step 1: Place the blood sample to be tested in the sample pool 201. The liquid level of the blood sample should be higher than the height of the injection valve 202, and 60% of the total volume of the blood sample should be located in the bottom compartment 207 of the sample pool. At this time, the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 are located at the first position, and the sample valve 204, reagent valve 303, washing solution valve 503, reaction valve 402, and discharge valve 405 are all in the closed state.

[0133] The microfluidic chip is centrifuged to separate blood cells into the bottom chamber 207 of the sample pool 201, while plasma remains in the top chamber 206 above the blood cells.

[0134] Step 2: Rotate the programmable module 103 to make the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 enter the second position respectively, so that the sample valve 204 and reaction valve 402 are in the open state, and the reagent valve 303, discharge valve 405, and washing solution valve 503 are in the closed state;

[0135] After the sample valve 202 is dissolved by soaking in plasma, the plasma enters each reaction cell 403 through sample channel 203, annular channel 8 and reaction channel 401 under centrifugation. Only plasma enters sample channel 203 here, and blood cells are deposited in the bottom chamber 207 of the sample cell and will not enter sample channel 203, so they will not interfere with the detection.

[0136] Step 3: After the plasma enters each reaction tank 403, stop centrifugation; rotate the programmable module 103 so that the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 respectively enter the third position, so that the sample valve 204, reagent valve 303, reaction valve 402, discharge valve 405, and washing solution valve 503 are all in the closed state;

[0137] The slgE antibody in the plasma reacts with the allergen-labeled magnetic microspheres in the reaction chamber 403 to obtain the magnetic microspheres that have been bound to the antibody. During the reaction, a high-frequency changing magnetic field can be applied to the reaction chamber 403 to accelerate the movement of the magnetic microspheres and accelerate the reaction.

[0138] Step 4: After the reaction is complete, rotate the programmable module 103 to move the sample valve control unit 1031, reagent valve control unit 1032, washing valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 to their fourth positions respectively. This opens the washing valve 503, reaction valve 402, and discharge valve 405, while closing the sample valve 204 and reagent valve 303. Centrifugation is then performed, allowing the washing solution in the washing tank 501 to flow through the reaction tank 403 and separate the unbound slgE antibody. In this step, the magnetic microspheres are controlled to adhere to the upper side of the reaction tank 403 to prevent them from being discharged into the waste liquid channel 404 with the reagents. The same applies to subsequent centrifugation / washing steps.

[0139] Step 5: After separation is completed, rotate the programmable module 103 to move the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 to the fifth position respectively, so that the sample valve 204, discharge valve 405, and washing solution valve 503 are closed, and the reagent valve 303 and reaction valve 402 are open, and centrifugation is performed to allow the fluorescently labeled mouse anti-human IgE antibody in reagent pool 301 to enter each reaction pool 403;

[0140] Step 6: After all the fluorescently labeled mouse anti-human IgE antibodies have entered each reaction cell 403, stop centrifugation and rotate the programmable module 103 to move the sample valve control unit 1031, reagent valve control unit 1032, washing valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 to the sixth position respectively, so that the sample valve 204, reagent valve 303, reaction valve 402, discharge valve 405, and washing valve 503 are closed, so that the fluorescently labeled mouse anti-human IgE antibodies react with the magnetic microspheres that have bound the antibodies;

[0141] Step 7: After the reaction is complete, rotate the programmable module 103 to move the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 to the seventh position respectively, so that the reaction valve 402, discharge valve 405, and washing solution valve 503 are in the open state, and the sample valve 204 and reagent valve 303 are in the closed state. Centrifugation is performed so that the washing solution in the washing solution tank 501 flows through the reaction tank 403 to separate the unbound fluorescently labeled mouse anti-human IgE antibody.

[0142] Step 8: After separation is complete, stop centrifugation and rotate the programmable module 103 to move the sample valve control unit 1031, reagent valve control unit 1032, washing solution valve control unit 1033, reaction valve control unit 1034, and discharge valve control unit 1035 to their eighth positions respectively, so that the sample valve 204, reagent valve 303, reaction valve 402, discharge valve 405, and washing solution valve 503 are in the closed state, that is, all valves are closed, and the microfluidic chip is sent into the fluorescence detection device for detection.

[0143] Compared with existing conventional detection chips, the microfluidic chip of Example 1 can bring the following advantages:

[0144] 1. Reduced sample requirements: 0.3 mL of whole blood can be used to react simultaneously to 60 allergens, with only about 2 μL of serum required for each allergen;

[0145] 2. It can achieve automatic operation. All reagents, washing solutions, etc. can be preset inside the microfluidic chip. Through the programmable module to control each valve and centrifugation operation, automatic addition and separation can be achieved.

[0146] 3. Short reaction time: By using high-concentration samples and accelerating the magnetic microspheres in the reaction cell with the help of a magnetic field, the reaction can be accelerated, and the reaction time generally only needs 20-30 minutes.

[0147] 4. High sensitivity: This microfluidic chip can use fluorescently labeled antibodies, which have higher sensitivity than commonly used color development methods such as enzyme-labeled antibodies and precipitation substrates.

Claims

1. A microfluidic chip for blood testing, characterized in that, The microfluidic chip for blood detection is disc-shaped and includes, from top to bottom, a chip body (101), an elastic membrane (102), and a programmable module (103). The chip body (101) is provided with a sample section, a reagent section, a reaction section, a washing solution section, a waste solution section, and an annular channel (8); the annular channel (8) is centered on the center of the microfluidic chip used for blood detection; the sample section, reagent section, and washing solution section are located inside the annular channel (8), the reaction section is located outside the annular channel (8), and the waste solution section is located outside the reaction section; The sample section includes at least one combination of sample pool (201) and sample channel (203), wherein the inlet of the sample channel (203) is connected to the sample pool (201) through a sample injection valve (202), and the outlet is connected to the annular channel (8); The reagent section includes at least one combination of reagent pool (301) and reagent channel (302), wherein the inlet and outlet of the reagent channel (302) are respectively connected to the reagent pool (301) and the annular channel (8); The reaction section includes a combination of 20-80 sets of reaction channels (401), reaction tanks (403), and waste liquid channels (404). The waste liquid section includes 20-80 waste liquid pools (601). The inlet and outlet of the reaction channel (401) are respectively connected to the annular channel (8) and the reaction tank (403). The inlet and outlet of the waste liquid channel (404) are respectively connected to the reaction tank (403) and the waste liquid pool (601). The washing liquid section includes at least one combination of washing liquid tank (501) and washing liquid channel (502); the inlet and outlet of the washing liquid channel (502) are respectively connected to the washing liquid tank (501) and the annular channel (8); The sample channel (203) is provided with a valve for controlling the corresponding channel between the sample injection valve (202) and the annular channel (8), the reagent channel (302), the reaction channel (401), the waste liquid channel (404), and the washing liquid channel (502); The programmable module (103) is rotatable and has a valve control section on its upper surface for controlling each valve. 2.The microfluidic chip for blood testing according to claim 1, wherein, The bottom surfaces of the annular channel (8), the reagent pool (301), the reaction pool (403), the washing solution pool (501), the sample channel (203), the reagent channel (302), the reaction channel (401), the waste liquid channel (404), and the washing solution channel (502) are respectively located on the lower surface of the chip body (101) and are sealed by the elastic membrane (102). 3.The microfluidic chip for blood testing according to claim 1, wherein, The microfluidic chip for blood detection also includes a chip base (104); the chip base (104) is located at the bottom of the center of the microfluidic chip for blood detection; The sample pool (201) includes a sample pool top compartment (206) and a sample pool bottom compartment (207) that are interconnected. The sample pool top compartment (206) is a through hole located at the center of the chip body (101), and the sample pool bottom compartment (207) is located inside the chip base (104). The horizontal position of the entrance of the sample channel (203) is located at the horizontal dividing line between the top compartment (206) and the bottom compartment (207) of the sample pool. 4.The microfluidic chip for blood testing according to claim 1, wherein, The injection valve (202) is a water-soluble valve and is located at the entrance of the sample channel (203).

5. The microfluidic chip for blood testing according to claim 1, wherein, The valve includes a valve ball and a groove corresponding to the upper and lower parts. The valve ball can be pushed up into the groove to close the corresponding channel. The valve control unit is located on the upper surface of the programmable module (103) at the position corresponding to the corresponding valve. The valve control unit includes several points, each point being either a protrusion or a space. The protrusion is a raised structure on the surface of the programmable module (103), and the space is a structure flush with the surface of the programmable module (103). When the protrusion is located below the corresponding valve, the protrusion lifts the corresponding valve ball, and the corresponding valve ball enters the corresponding groove, thereby closing the corresponding channel. When the empty space is located below the corresponding valve, the empty space does not lift the corresponding valve ball, and the corresponding valve ball does not enter the groove, thereby keeping the corresponding channel in the open state. 6.The microfluidic chip for blood detection according to claim 1 or 5, characterized in that, The valves include a sample valve (204), a reagent valve (303), a reaction valve (402), a discharge valve (405), and a washing solution valve (503), which are used to control the sample channel (203), the reagent channel (302), the reaction channel (401), the waste liquid channel (404), and the washing solution channel (502), respectively. The sample valve (204) includes a sample valve ball (208) and a sample valve groove (209) positioned vertically. The sample valve ball (208) is located in a slot at the bottom of the sample channel (203), and the sample valve groove (209) is located at the top of the sample channel (203). It is an upward groove on the chip body (101) at the corresponding position. The size of the sample valve ball (208) is larger than the size of the sample channel (203). When the sample valve ball (208) is lifted, it enters the sample valve groove (209) to close the sample channel (203). The valve control unit includes a sample valve control unit (1031) corresponding to the sample valve (204). The sample valve control unit (1031) is used to control the opening and closing of the sample valve (204). The sample valve control unit (1031) includes 8 points, which are, in order, a protrusion, an empty position, a protrusion, a protrusion, a protrusion, a protrusion, a protrusion, a protrusion, a protrusion. The reagent valve (303) includes a reagent valve ball (304) and a reagent valve groove (305) positioned vertically. The reagent valve ball (304) is located in a slot at the bottom of the reagent channel (302), and the reagent valve groove (305) is located at the top of the reagent channel (302). It is an upward groove on the chip body (101) at the corresponding position. The size of the reagent valve ball (304) is larger than the size of the reagent channel (302). When the reagent valve ball (304) is lifted, it enters the reagent valve groove (305) to close the reagent channel (302). The valve control unit includes a reagent valve control unit (1032) corresponding to the reagent valve (303). The reagent valve control unit (1032) is used to control the opening and closing of the reagent valve (303). The reagent valve control unit (1032) includes 8 points, which are, in order, protrusion, protrusion, protrusion, empty, protrusion, protrusion, and protrusion. The reaction valve (402) includes a reaction valve ball (406) and a reaction valve groove (407) positioned vertically. The reaction valve ball (406) is located in a slot at the bottom of the reaction channel (401), and the reaction valve groove (407) is located at the top of the reaction channel (401). It is an upward groove on the chip body (101) at the corresponding position. The size of the reaction valve ball (406) is larger than the size of the reaction channel (401). When the reaction valve ball (406) is lifted, it enters the reaction valve groove (407) to close the reaction channel (401). The valve control unit includes a reaction valve control unit (1034) corresponding to the reaction valve (402). The reaction valve control unit (1034) is used to control the opening and closing of the reaction valve (402). The reaction valve control unit (1034) includes 8 points, which are, in order, protrusion, empty, protrusion, empty, empty, protrusion, empty, and protrusion. The discharge valve (405) includes a discharge valve ball (408) and a discharge valve groove (409) positioned vertically. The discharge valve ball (408) is located in a slot at the bottom of the waste liquid channel (404), and the discharge valve groove (409) is located at the top of the waste liquid channel (404). It is an upward groove on the chip body (101) at the corresponding position. The size of the discharge valve ball (408) is larger than the size of the waste liquid channel (404). When the discharge valve ball (408) is lifted, it enters the discharge valve groove (409) to close the waste liquid channel (404). The valve control unit includes a discharge valve control unit (1035) corresponding to the discharge valve (405). The discharge valve control unit (1035) is used to control the opening and closing of the discharge valve (405). The discharge valve control unit (1035) includes 8 points, which are, in order, protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, and protrusion. The washing valve (503) includes a washing valve ball (504) and a washing valve groove (505) positioned vertically. The washing valve ball (504) is located in a slot at the bottom of the washing channel (502), and the washing valve groove (505) is located at the top of the washing channel (502). It is an upward groove on the chip body (101) at the corresponding position. The size of the washing valve ball (504) is larger than the size of the washing channel (502). When the washing valve ball (504) is lifted, it enters the washing valve groove (505) to close the washing channel (502). The valve control unit includes a washing liquid valve control unit (1033) corresponding to the washing liquid valve (503). The washing liquid valve control unit (1033) is used to control the opening and closing of the washing liquid valve (503). The washing liquid valve control unit (1033) includes 8 points, which are, in order, protrusion, protrusion, protrusion, empty, protrusion, protrusion, empty, and protrusion.

7. The microfluidic chip for blood testing according to claim 1, wherein, The sample channel (203), the reagent channel (302), the reaction channel (401), the waste liquid channel (404), and the washing liquid channel (502) are arranged along the radial direction of the microfluidic chip used for blood detection; The combination of 20-80 sets of reaction channels (401), reaction pools (403) and waste liquid channels (404) and 20-80 waste liquid pools (601) are evenly distributed along the circumference of the microfluidic chip used for blood detection. 8.The microfluidic chip for blood testing of claim 1, wherein, The reaction tank (403) is equipped with magnetic microspheres; The programmable module (103) is provided with a fluorescence observation hole (108) at the position corresponding to the reaction cell (403).

9. The microfluidic chip for blood testing according to claim 6, wherein, The microfluidic chip for blood detection also includes a positioning track (105), which is located between the elastic membrane (102) and the programmable module (103); The positioning track (105) has five tracks, which correspond to the sample valve control unit (1031), reagent valve control unit (1032), washing liquid valve control unit (1033), reaction valve control unit (1034), and discharge valve control unit (1035), respectively, so that it can rotate along the track.

10. The microfluidic chip for blood testing according to claim 1, wherein, The microfluidic chip for blood detection is provided with a positioning buckle (701) and a rotating buckle (702) on its outer periphery, and the rotating buckle (702) is connected to the programmable module (103).

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