A device for breaking up a biological cell aggregate

Abstract

The application discloses a biological cell cluster breaking device, and relates to the field of biological cell processing. The device comprises a driving mechanism, a hose and a container tank. The driving mechanism is used for driving liquid transmission of the hose. The hose is arranged on the driving mechanism. One end of the hose is communicated with the container tank, and the other end of the hose is communicated with a fluid. The end of the hose in the container tank is connected with a breaking mechanism. The breaking mechanism is provided with a plurality of directional output ports towards the inner wall of the container tank. The driving mechanism is provided with a pressing part used for controlling the pressing degree of the hose. The application has the effects of effectively breaking the biological cell cluster, controllable liquid transmission, convenient component dismounting and maintenance.

Description

Technical Field

[0001] This application relates to the field of biological cell processing technology, and in particular to a device for breaking up biological cell agglomerations. Background Technology

[0002] With the continuous development of biotechnology, the manipulation and processing of biological cells have become increasingly sophisticated and complex. Cell aggregation is a common phenomenon in the processes of cell culture and isolation, and aggregated cells can affect cell growth, metabolism, and subsequent experimental analysis. Therefore, effectively breaking up cell aggregates has become a key research direction in the field of cell biology. This not only relates to the accuracy and reliability of cell experiments but also has a significant impact on practical applications such as biopharmaceuticals and cell therapy.

[0003] In the past, various methods have been conventionally used to address the problem of cell aggregation. One method involves mechanical agitation, using a rotating impeller in the cell culture medium to generate water flow that attempts to break up the aggregated cells. This method is relatively simple to operate and is widely used in large-scale cell cultures. Another method is sonication, which utilizes the cavitation effect of ultrasound to generate tiny bubbles in the cell culture medium that rapidly burst, generating impact force to break up cell aggregates. A simple liquid impact method involves spraying cell culture medium through a pipe at a certain flow rate, using the impact force of the liquid to break up cell aggregates.

[0004] However, these existing conventional methods have significant drawbacks. Mechanical agitation can easily damage cells because the high-speed rotation of the agitator can generate significant shear forces, disrupting cell structure and function. While sonication can generate strong impact, the ultrasonic energy is difficult to control precisely, potentially causing excessive damage to cells and affecting their viability. Simple liquid impact methods are ineffective at breaking up tightly clustered cells and cannot provide targeted treatment based on the characteristics of different cells. Utility Model Content

[0005] To address the issues of conventional methods causing cell damage and affecting cell observation, the poor effectiveness of simple liquid impact, and the inability to target different cells, this application provides a device for breaking up biological cell agglomerations.

[0006] The biological cell aggregation and dispersing device provided in this application adopts the following technical solution: A device for dispersing aggregated biological cells includes a drive mechanism, a hose, and a container. The drive mechanism is used to drive the liquid transported through the hose. The hose is arranged on the drive mechanism, with one end of the hose connected to the container and the other end connected to the fluid. A dispersing mechanism is connected to the end of the hose located inside the container. The dispersing mechanism has multiple outlets facing the inner wall of the container. A clamping element is arranged on the drive mechanism to control the degree of compression of the hose.

[0007] By adopting the above technical solution, the driving mechanism drives the liquid transport connected by the hose, allowing the fluid to enter the hose and be transported to the container. The multiple output ports of the dispersing mechanism can flush the fluid from multiple directions to the inner wall of the container, which helps to break up the clustered biological cells. The clamping component can control the degree of clamping of the hose, and different clamping distances can be selected according to different cells to disperse the cells.

[0008] Optionally, the drive mechanism includes a control motor, a clamping wheel detachably mounted on the output end of the control motor, a fixing plate mounted on the control motor, a cover mounted on the fixing plate, and a fixing seat mounted on the cover. The clamping member slides on the cover, the side of the clamping member contacts the clamping wheel, and the side of the clamping member away from the clamping wheel contacts the hose.

[0009] By adopting the above technical solution, the drive mechanism can drive the liquid transport connected by the hose. The control motor drives the pressure wheel to rotate. The pressure wheel is a cam, which makes the pressure member slide on the cover and contact the hose, thereby controlling the degree of pressure of the hose and realizing the control of liquid transport. Then, different pressure distances can be selected according to different cells to break up the cells.

[0010] Optionally, the fixed base has a groove, and the cover has a guide groove on the side facing the fixed base. The guide groove and the groove are connected. The clamping member includes a pressure plate that slides in the guide groove. The pressure plate passes through the fixed base and contacts the hose. The side of the pressure plate contacts the clamping wheel. The side of the pressure plate away from the clamping wheel contacts the hose. A spring is provided on the side of the pressure plate. The end of the spring away from the pressure plate is connected to the fixed base.

[0011] By adopting the above technical solution, fluid is transported into the container through a hose. The outlet of the flushing mechanism facing multiple directions toward the inner wall of the container can flush away the aggregated cells. The pressure plate in the clamping component can slide in the guide groove. Under the action of the spring, the degree of clamping on the hose can be adjusted according to the rotation of the clamping wheel to ensure the clamping position and press the hose into a microporous structure. Different clamping distances are selected according to different cells to disperse the cells.

[0012] Optionally, the fixing seat has a convex edge and a recessed part, and the hose is snapped between the pressure plate and the recessed part; under the action of the pressure wheel rotation, the pressure plate slides along the guide groove to the groove, and the gap formed by the pressure plate and the convex edge is larger than the diameter of the hose.

[0013] By adopting the above technical solution, the hose is clamped between the pressure plate and the recess, so that the hose is fixed and stable in the device; under the action of the pressure wheel rotation, the pressure plate slides along the guide groove to the recess, and the gap formed by the pressure plate and the convex edge is larger than the diameter of the hose, which can realize the control of the degree of hose compression, and then select different compression distances according to different cells.

[0014] Optionally, the flushing mechanism includes a detachable filter ball, the hose is connected to the inlet of the filter ball, and the outlet of the filter ball is connected to branch pipes in different directions.

[0015] By adopting the above technical solution, the detachable filter ball is easy to clean and maintain, and the branch pipes in different directions allow the fluid to be output from multiple directions, which can better break up the agglomerated biological cells in the container.

[0016] Optionally, the filter ball is divided into an upper ball and a lower ball, which are connected by a locking fastener. The hose is threadedly fitted to the upper ball, and the lower ball has multiple filter ports. The branch pipe passes through the filter ports, and the branch pipe and multiple hoses are connected by a multi-port connecting pipe.

[0017] By adopting the above technical solution, the filter ball is divided into an upper ball and a lower ball, which are connected by a locking fastener, making it convenient to disassemble and assemble the filter ball, and easy to maintain and clean. The hose and the upper ball are threaded together, making the connection between the hose and the filter ball stable and easy to install and disassemble. The lower ball has multiple filter ports, and the branch pipes pass through the filter ports, which can realize output in different directions. The branch pipes and multiple hoses are connected by a multi-port connecting pipe, which can distribute the fluid among multiple branch pipes. Combined with the multiple output ports of the dispersing mechanism, it can better break up the agglomerated biological cells.

[0018] Optionally, the multi-port connecting pipe is a tee pipe, a four-way pipe, or a multi-port pipe.

[0019] By adopting the above technical solution, using a three-way pipe, four-way pipe or multi-way pipe as a multi-port connecting pipe, it is possible to connect the branch pipe with multiple hoses, so that the dispersing mechanism can output liquid from multiple directions, and better disperse the aggregated biological cells.

[0020] Optionally, the cross-section of the branch pipe is wavy or straight, the hose is made of rubber, and the branch pipe is a rigid pipe.

[0021] By adopting the above technical solutions, the branch pipe with a wavy or straight cross section can change the fluid flow state, which is conducive to cell dispersal; the rubber hose has good flexibility, which makes it easy for the drive mechanism to control its compression degree to adjust the liquid transmission; the branch pipe with rigid pipe material can ensure structural stability, maintain the fluid output direction and effect, and effectively realize the dispersal of biological cell agglomeration.

[0022] In summary, this application includes at least one of the following beneficial technical effects: 1. By driving the liquid transport through the hose via the drive mechanism, clumped cells can be broken up, preventing cell clumping from affecting cell growth, metabolism, and subsequent experimental analysis; 2. The clamping components on the drive mechanism can control the degree of clamping of the hose. Different clamping distances can be selected according to different cells to achieve targeted cell dispersal. 3. The dispersing mechanism has multiple outlets facing the inner wall of the container, which can disperse the cells from multiple directions and improve the cell dispersing effect. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure shown in this application.

[0025] Figure 2 This is a schematic diagram of the drive mechanism shown in this application.

[0026] Figure 3 This is a cross-sectional view showing the overall structure of this application.

[0027] Reference numerals: 1. Drive mechanism; 2. Hose; 3. Container; 4. Flushing mechanism; 5. Clamping component; 11. Control motor; 12. Clamping wheel; 13. Fixing plate; 14. Cover; 15. Fixing base; 6. Groove; 7. Guide groove; 51. Pressure plate; 52. Spring; 151. Protruding edge; 152. Recess; 41. Filter ball; 42. Branch pipe; 411. Upper ball; 412. Lower ball; 413. Multi-port connecting pipe. Detailed Implementation

[0028] The following is in conjunction with the appendix Figure 1-3 This application will be described in further detail.

[0029] This application discloses a device for breaking up biological cell agglomerations.

[0030] Reference Figure 1 The system includes a drive mechanism 1, a hose 2, and a container 3. The drive mechanism 1 is used to drive the liquid transport connected by the hose 2. The hose 2 is arranged on the drive mechanism 1, with one end connected to the container 3 and the other end connected to the fluid. The end of the hose 2 located inside the container 3 is connected to a dispersing mechanism 4. The dispersing mechanism 4 has multiple outlets facing the inner wall of the container 3. The drive mechanism 1 is equipped with a clamping element 5 for controlling the clamping degree of the hose 2, which achieves the effect of targeted dispersing of cell clusters according to different cell characteristics and reducing cell damage. The drive mechanism 1 can control the liquid transport, the clamping element 5 can adjust the clamping degree of the hose 2 to adapt to different cells, and the multi-directional outlets of the dispersing mechanism 4 can more effectively disperse cell clusters.

[0031] See Figure 2 As shown, the drive mechanism 1 includes a control motor 11, a clamping roller 12 detachably mounted on the output end of the control motor 11, a fixing plate 13 mounted on the control motor 11, a cover 14 mounted on the fixing plate 13, and a fixing seat 15 mounted on the cover 14. The control motor 11 is typically a stepper motor, capable of precisely controlling the rotation angle and stroke. The clamping roller 12 is generally elliptical, made of rubber to increase friction with the clamping component 5, ensuring transmission efficiency, and is wear-resistant and elastic. Different clamping distances can be selected for different cells, allowing for the selection of clamping rollers 12 of varying sizes to achieve different degrees of clamping on the hose 2. The fixing plate 13 has good strength and corrosion resistance, and is fixedly connected to the control motor 11 by bolts. The cover 14 is connected to the fixing plate 13 by clips or bolts, serving to protect internal components. The fixing seat 15 has good stability and is connected to the cover 14 by welding or bolts. The clamping component 5 slides on the cover 14. The side of the clamping component 5 is in contact with the clamping wheel 12, and the side of the clamping component 5 away from the clamping wheel 12 is in contact with the hose 2. The clamping component 5 is generally a plate-shaped structure with good wear resistance and self-lubrication. The clamping component 5 can slide on the cover 14 by the cooperation of a guide rail and a slider. The guide rail is installed on the cover 14, and the slider is installed on the clamping component 5 to ensure the stability and accuracy of the sliding of the clamping component 5.

[0032] See Figure 3As shown, a groove 6 is provided on the fixed base 15, and a guide groove 7 is provided on the side of the cover 14 facing the fixed base 15. The guide groove 7 and the groove 6 are connected. The pressure plate 51 passes through the fixed base 15 and contacts the hose 2. The pressure plate 51 is also a plate structure. The side of the pressure plate 51 is in contact with the pressure roller 12, and the side of the pressure plate 51 away from the pressure roller 12 is in contact with the hose 2. A spring 52 is provided on the side of the pressure plate 51. The end of the spring 52 away from the pressure plate 51 is connected to the fixed seat 15. The spring 52 is generally a helical spring 52. The function of the spring 52 is to return the pressure plate 51 to its initial position when the pressure roller 12 does not apply pressure, so as to ensure that the hose 2 returns to its normal state. The fixed seat 15 has a convex edge 151 and a recessed part 152. The hose 2 is clamped between the pressure plate 51 and the recessed part 152. Under the action of the rotation of the pressure roller 12, the pressure plate 51 slides along the guide groove 7 to the groove 6. The gap formed by the pressure plate 51 and the convex edge 151 is larger than the diameter of the hose 2, so that when the pressure roller 12 rotates, the pressure plate 51 can slide in the guide groove 7 and the groove 6, changing the degree of compression on the hose 2, thereby controlling the flow of liquid in the hose 2.

[0033] See Figure 3 As shown, the flushing mechanism 4 includes a detachable filter ball 41, a hose 2 connected to the inlet of the filter ball 41, and branch pipes 42 in different directions connected to the outlet of the filter ball 41. The detachable filter ball 41 is generally composed of upper and lower parts, and the material can be transparent plastic, such as plexiglass, for easy observation of the internal condition. The branch pipes 42 are usually rigid pipes, such as glass or stainless steel pipes, to ensure the stability of liquid transmission. The cross-section of the branch pipes 42 is wavy or straight. The wavy cross-section of the branch pipe 42 can increase the disturbance during liquid flow and improve the effect of breaking up cell clusters; the straight cross-section of the branch pipe 42 allows the liquid to flow out more smoothly. The filter ball 41 is divided into an upper ball 411 and a lower ball 412, which are connected by a locking device, which can be a snap-fit ​​or a threaded connection, for easy disassembly and cleaning of the filter ball 41. The hose 2 and the upper ball 411 are threaded together to ensure the sealing of the connection. The lower sphere 412 has multiple filter ports, and branch pipes 42 pass through the filter ports. The branch pipes 42 and multiple flexible hoses 2 are connected by a multi-port connecting pipe 413. The multi-port connecting pipe 413 can be a three-way pipe, a four-way pipe, or a multi-way pipe. Different connecting pipes can be selected according to actual needs to achieve liquid output in different directions.

[0034] The implementation principle of the biological cell agglomeration dispersing device in this application embodiment is as follows: the control motor 11 and pressure roller 12 of the drive mechanism 1 can precisely control the degree of compression of the hose 2. According to the characteristics of different cells, the degree to which the hose 2 is compressed into a microporous structure can be adjusted, thereby achieving targeted cell agglomeration dispersal. This avoids excessive damage to cells caused by mechanical stirring, ultrasonic treatment and other methods in the prior art, and improves cell activity. The detachable filter ball 41 and multi-directional branch pipe 42 of the dispersing mechanism 4 can make the cell fluid impact the inner wall of the container 3 from multiple directions, more effectively dispersing cell agglomerates. The detachable structure is convenient for cleaning and maintenance. The overall device has a simple structure and is easy to operate. It has effectively improved the prior art, can meet the needs of the biological cell field for cell agglomeration dispersal, and improves the accuracy and reliability of cell experiments.

[0035] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms "first," "second," "third," and similar terms used in this application specification and claims do not indicate any order, quantity, or importance, but are merely used to distinguish different components. The terms "an" or "a" and similar terms do not indicate a quantity limitation, but rather indicate the presence of at least one. The terms "comprising" or "including" and similar terms mean that the elements or objects preceding "comprising" or "including" encompass the elements or objects listed following "comprising" or "including" and their equivalents, and do not exclude other elements or objects. "Above," "below," "left," "right," etc., are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0036] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A device for breaking up biological cell agglomerations, characterized in that: The device includes a drive mechanism (1), a hose (2), and a container (3). The drive mechanism (1) is used to drive the liquid transport connected by the hose (2). The hose (2) is arranged on the drive mechanism (1). One end of the hose (2) is connected to the container (3), and the other end of the hose (2) is connected to the fluid. The end of the hose (2) located inside the container (3) is connected to a dispersing mechanism (4). The dispersing mechanism (4) has multiple outlets facing the inner wall of the container (3). The drive mechanism (1) is provided with a clamping member (5) for controlling the degree of compression of the hose (2).

2. The biological cell agglomeration dispersing device according to claim 1, characterized in that: The drive mechanism (1) includes a control motor (11), a clamping wheel (12) detachably mounted on the output end of the control motor (11), a fixing plate (13) mounted on the control motor (11), a cover (14) mounted on the fixing plate (13), and a fixing seat (15) mounted on the cover (14). The clamping member (5) slides on the cover (14), the side of the clamping member (5) is in contact with the clamping wheel (12), and the side of the clamping member (5) away from the clamping wheel (12) is in contact with the hose (2).

3. The biological cell agglomeration dispersing device according to claim 2, characterized in that: The fixed base (15) has a groove (6) and the cover (14) has a guide groove (7) on the side facing the fixed base (15). The guide groove (7) and the groove (6) are connected. The clamping member (5) includes a pressure plate (51) that slides in the guide groove (7). The pressure plate (51) passes through the fixed base (15) and contacts the hose (2). The side of the pressure plate (51) contacts the clamping wheel (12). The side of the pressure plate (51) away from the clamping wheel (12) contacts the hose (2). A spring (52) is provided on the side of the pressure plate (51). The end of the spring (52) away from the pressure plate (51) is connected to the fixed base (15).

4. The biological cell agglomeration dispersing device according to claim 3, characterized in that: The fixing seat (15) has a convex edge (151) and a recess (152). The hose (2) is snapped between the pressure plate (51) and the recess (152). Under the action of the pressure wheel (12) rotating, the pressure plate (51) slides along the guide groove (7) to the groove (6). The gap formed by the pressure plate (51) and the convex edge (151) is larger than the diameter of the hose (2).

5. The biological cell agglomeration dispersing device according to claim 1, characterized in that: The purging mechanism (4) includes a detachable filter ball (41), the hose (2) is connected to the inlet of the filter ball (41), and the outlet of the filter ball (41) is connected to branch pipes (42) in different directions.

6. The biological cell agglomeration dispersing device according to claim 5, characterized in that: The filter ball (41) is divided into an upper ball (411) and a lower ball (412). The upper ball (411) and the lower ball (412) are connected by a locking fastener. The hose (2) is threadedly fitted to the upper ball (411). The lower ball (412) has multiple filter ports. The branch pipe (42) passes through the filter ports. The branch pipe (42) and the multiple hoses (2) are connected by a multi-port connecting pipe (413).

7. The biological cell agglomeration dispersing device according to claim 6, characterized in that: The multi-port connecting pipe (413) is a tee pipe, a four-way pipe, or a multi-port pipe.

8. The biological cell agglomeration dispersing device according to claim 5, characterized in that: The cross-section of the branch pipe (42) is wavy or straight, the hose (2) is made of rubber, and the branch pipe (42) is a rigid pipe.

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