A nucleic acid extraction and amplification integrated device
Patent Information
- Application Number
- CN202522239995.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-23
- Publication Date
- 2026-09-15
- Estimated Expiration
- 2035-10-23
AI Technical Summary
核酸提取的目的是获得高纯度、完整性良好的核酸，以便后续分析；扩增则是在样本中起始核酸量极低时，通过放大核酸的数量，使其达到可检测、可分析的水平，目前核酸在提取以及扩增环节中，通常将其提取到核酸样本，放置在扩增前安放的位置，在进行扩增前期，需要将放置后的核酸样本，逐一放置到扩增装置内进行扩增处理，由于样本提取放置与扩增之间操作过程中，涉及到相关技术人员，一方面工艺流程不顺畅，间接性操作，相对效率低下，另一方面涉及到的相关技术人员，也增加了涉及人员的工作量以及工作负荷程度，进而降低了核酸提取扩增一体化装置的使用效果，也间接影响到核酸提取扩增一体化装置的效率
本装置通过辅助组件中的电机 B 驱动转盘 B 转动，配合电动伸缩杆的精准伸缩与夹持气缸的稳定夹持，能够自动完成提取样本试瓶从提取样本放置架到扩增腔内部扩增放置架的转移过程，无需人工手动介入，有效实现了核酸提取环节与扩增环节的无缝衔接，大幅减少了两个环节之间的操作间隔时间，显著提升了整体核酸检测的效率，同时也减少了人工操作的步骤，降低了相关技术人员的工作强度与工作负荷，使工作人员可以将更多精力投入到样本质量把控、设备运行状态监测等关键工作环节，进而提升整体核酸检测工作的质量；
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Figure CN224754415U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of nucleic acid detection technology, specifically to an integrated device for nucleic acid extraction and amplification. Background Technology
[0002] Nucleic acid extraction and amplification refers to the process of isolating and purifying nucleic acids (DNA or RNA) from biological samples, and then exponentially amplifying them using specific methods for detection, analysis, and application. The purpose of nucleic acid extraction is to obtain high-purity, intact nucleic acids for subsequent analysis; amplification, on the other hand, involves increasing the quantity of nucleic acids in a sample from an initial low level to a detectable and analyzable level. Currently, in both extraction and amplification, nucleic acid samples are typically extracted and placed in a pre-amplification position. Before amplification, these samples need to be individually placed into the amplification device. Because the process between sample extraction / placement and amplification involves technical personnel, the workflow is inefficient due to indirect operations. Furthermore, the increased workload of these personnel reduces the effectiveness of the integrated nucleic acid extraction and amplification device and indirectly affects its efficiency.
[0003] Therefore, it is necessary to develop an integrated nucleic acid extraction and amplification device. Utility Model Content
[0004] The purpose of this invention is to provide an integrated nucleic acid extraction and amplification device to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, this utility model provides the following technical solution: an integrated nucleic acid extraction and amplification device, comprising an operating frame, a support mounted on one side of the bottom of the operating frame, and an amplification chamber mounted on one side of the top of the operating frame; An auxiliary component is installed above the operating frame. The auxiliary component includes a mounting frame, and a turntable B is rotatably connected to the top center of the mounting frame.
[0006] Preferably, a motor A is mounted at the bottom of the bracket, and a rotating shaft A is mounted at the output end of the motor A.
[0007] Preferably, a synchronous wheel A is provided on the outer wall of the rotating shaft A, and a turntable A is installed at the top of the rotating shaft A.
[0008] Preferably, a sample extraction rack is installed on the top of the turntable A, and sample extraction bottles are slidably connected inside the sample extraction rack.
[0009] Preferably, an amplification placement rack is rotatably connected inside the amplification chamber, a rotating shaft B is installed at the bottom center of the amplification placement rack, a synchronous wheel B is provided on the outer wall of the rotating shaft B, a sealing cover is installed on the top of the amplification chamber, and a baffle is rotatably connected to one side of the top of the sealing cover.
[0010] Preferably, screws are installed on both outer walls of the amplification chamber, and fixing seats are provided on both outer walls of the sealing cover. The interior of the fixing seat is slidably connected to the outer wall of the screw, and a knob is threadedly connected to the upper part of the outer wall of the screw.
[0011] Preferably, a motor B is installed above the turntable B, and the output end of the motor B is fixedly connected to the top center of the turntable B.
[0012] Preferably, an electric telescopic rod is installed on one side of the top of the turntable B, and a clamping cylinder is installed at the output end of the electric telescopic rod.
[0013] Compared with the prior art, the beneficial effects of this utility model are: This device drives the turntable B to rotate via motor B in the auxiliary components. Combined with the precise extension and retraction of the electric telescopic rod and the stable clamping of the clamping cylinder, it can automatically complete the transfer process of the extracted sample vial from the sample placement rack to the amplification placement rack inside the amplification chamber without manual intervention. This effectively achieves seamless connection between the nucleic acid extraction and amplification processes, significantly reducing the operation interval between the two processes and significantly improving the overall efficiency of nucleic acid testing. At the same time, it reduces the number of manual operation steps, lowers the workload and intensity of related technical personnel, and allows staff to devote more energy to key tasks such as sample quality control and equipment operation status monitoring, thereby improving the overall quality of nucleic acid testing. The clamping cylinder of this device can stably hold the sample vials, preventing them from shaking or tilting during sample transfer and effectively preventing sample spillage. The precise extension and retraction of the electric telescopic rod and the stable rotation of turntable B ensure a fixed and controllable sample transfer path, reducing the contact time between the sample and the external environment during transfer, lowering the risk of sample contamination, ensuring sample quality, and thus improving the accuracy of nucleic acid test results. Meanwhile, the sealing cap at the top of the amplification chamber is slidably connected to the screws on the outer wall of the amplification chamber via fixing seats on both sides, and can be quickly fixed and disassembled using a knob above the screws. This convenient operation and good sealing effect ensure stable environmental parameters such as temperature and pressure inside the amplification chamber, providing suitable conditions for nucleic acid amplification reaction and improving amplification efficiency. Both the sample extraction rack and the amplification rack can accommodate multiple sample vials simultaneously, enabling simultaneous processing of multiple samples to meet the needs of batch nucleic acid testing and further enhancing the device's sample processing capacity. Attached Figure Description
[0014] Figure 1 This is a top view of the overall structure of the present invention; Figure 2 This is a bottom view of the overall structure of the present invention; Figure 3 Exploded view of a selected portion of the structure provided for this utility model; Figure 4 This is an enlarged schematic diagram of a selected portion of the structure provided by this utility model.
[0015] In the diagram: 1. Operating frame; 2. Support; 201. Motor A; 202. Rotating shaft A; 203. Synchronous pulley A; 204. Turntable A; 205. Sample extraction rack; 206. Sample extraction vial; 3. Amplification chamber; 301. Amplification rack; 302. Rotating shaft B; 303. Synchronous pulley B; 304. Sealing cap; 305. Baffle; 306. Screw; 307. Fixing base; 308. Knob; 4. Mounting frame; 401. Turntable B; 402. Motor B; 403. Electric telescopic rod; 404. Clamping cylinder. Detailed Implementation
[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0017] This utility model provides the following technical solution: an integrated nucleic acid extraction and amplification device, please refer to [link / reference]. Figures 1-4The system includes an operating frame 1, a support 2 mounted on one side of the bottom of the operating frame 1, a motor A201 mounted on the bottom of the support 2, a rotating shaft A202 mounted on the output end of the motor A201, a synchronous wheel A203 mounted on the outer wall of the rotating shaft A202, a turntable A204 mounted on the top of the rotating shaft A202, a sample extraction placement rack 205 mounted on the top of the turntable A204, sample extraction bottles 206 slidably connected inside the sample extraction placement rack 205, and an amplification chamber 3 mounted on one side of the top of the operating frame 1. The amplification chamber 3 is rotated inside... An amplification placement rack 301 is connected to the amplification placement rack 301. A rotating shaft B302 is installed at the bottom center of the amplification placement rack 301. A synchronous wheel B303 is provided on the outer wall of the rotating shaft B302. A sealing cover 304 is installed on the top of the amplification chamber 3. A baffle 305 is rotatably connected to one side of the top of the sealing cover 304. Screws 306 are installed on both outer walls of the amplification chamber 3. Fixing seats 307 are provided on both outer walls of the sealing cover 304. The interior of the fixing seat 307 is slidably connected to the outer wall of the screw 306. A knob 308 is threadedly connected to the upper part of the outer wall of the screw 306. An auxiliary component is installed above the operating frame 1. The auxiliary component includes a mounting frame 4. A turntable B401 is rotatably connected to the top center of the mounting frame 4. A motor B402 is installed above the turntable B401. The output end of the motor B402 is fixedly connected to the top center of the turntable B401. An electric telescopic rod 403 is installed on one side of the top of the turntable B401. A clamping cylinder 404 is installed at the output end of the electric telescopic rod 403.
[0018] Working Principle: When using this utility model, in the nucleic acid extraction and amplification integrated device for nucleic acid detection, firstly, the extracted samples are placed into multiple sample extraction vials 206. Then, these sample extraction vials 206 are slid one by one into the sample extraction rack 205, ensuring that each sample extraction vial 206 is stably placed within the rack 205 without tilting or shaking. Next, the motor A201 installed at the bottom of the support 2 is started. The output of motor A201 drives the rotating shaft A202 fixedly connected to it to start rotating. The top of shaft A202 is fixedly connected to turntable A204, and a sample placement rack 205 is installed on the top of turntable A204. Therefore, when shaft A202 rotates, it will synchronously drive turntable A204 and the sample placement rack 205 on top of turntable A204 to rotate together. According to the initial position of clamping cylinder 404 in the auxiliary component, by precisely controlling the speed and rotation angle of motor A201, one of the sample bottles 206 to be transferred on the sample placement rack 205 is rotated to the position corresponding to clamping cylinder 404, preparing for the subsequent sample transfer work; then the auxiliary component is started. The motor B402, mounted above the mounting bracket 4, drives the turntable B401, which is fixedly connected to it, to rotate. The rotation of the turntable B401 causes the electric telescopic rod 403 mounted on its top side and the clamping cylinder 404 mounted on its output end to rotate together until the clamping cylinder 404 rotates to a position directly above the previously positioned sample extraction vial 206. Then, the electric telescopic rod 403 extends, causing the clamping cylinder 404 to move downwards until its grippers are firmly against the outer wall of the sample extraction vial 206. When the sample bottle 206 is firmly clamped, the clamping cylinder 404 starts to work, and the grippers of the clamping cylinder 404 close, thus stably clamping the sample extraction bottle 206. After the clamping cylinder 404 confirms that the sample extraction bottle 206 is clamped, the electric telescopic rod 403 starts to retract. The retraction of the electric telescopic rod 403 will remove the sample extraction bottle 206 from the inside of the sample extraction placement rack 205. After the sample extraction bottle 206 is removed, the motor B402 is started again. The motor B402 drives the turntable B401 to continue to rotate, and the clamping cylinder 404 holding the sample extraction bottle 206 is transferred to the position directly above the amplification chamber 3. Before transferring the sample vial 206 to the amplification chamber 3, loosen the knobs 308 at the top of the screws 306 on both sides of the outer wall of the amplification chamber 3. Then slide the sealing cap 304 upwards. Since the fixing seats 307 on both sides of the sealing cap 304 are slidably connected to the screws 306, the sealing cap 304 will move upwards along the screws 306 and open. Next, rotate the baffle 305 rotatably connected to the top side of the sealing cap 304 to fully expose the amplification placement rack 301 installed inside the amplification chamber 3. When the sample vial 206 is transferred to the amplification chamber 3, loosen the knobs 308 at the top of the screws 306 on both sides of the amplification chamber 3. After being transported to the top of the amplification chamber 3, the electric telescopic rod 403 is extended again. The extension of the electric telescopic rod 403 will drive the clamping cylinder 404 holding the sample extraction bottle 206 to move downward, accurately placing the sample extraction bottle 206 into the corresponding placement position inside the amplification placement rack 301. After the sample extraction bottle 206 is placed in place, the clamping jaws of the clamping cylinder 404 are opened to release the clamping of the sample extraction bottle 206. Then, the electric telescopic rod 403 is retracted, driving the clamping cylinder 404 to return to its original position. Following the same steps described above, transfer all sample vials 206 from the sample placement rack 205 to the amplification placement rack 301 in sequence. After all sample vials have been transferred, first rotate the baffle 305 at the top of the sealing cap 304 back to its original position, then slide the sealing cap 304 downwards so that the fixing seats 307 on both sides of the sealing cap 304 are once again in contact with the screw 306. Next, tighten the knob 308 at the top of the screw 306. Through the squeezing action of the knob 308 on the fixing seats 307, the sealing cap 304 is firmly fixed to the top of the amplification chamber 3, ensuring that the amplification chamber 3 is sealed. Then, according to the specific requirements of the nucleic acid amplification reaction, set the parameters such as temperature, reaction time, and pressure inside the amplification chamber 3 on the relevant control equipment. After setting, start the amplification-related equipment and begin the nucleic acid amplification reaction. During the nucleic acid amplification reaction, the synchronous pulleys B303 on the outer wall of shaft B302 and A202 on shaft A202 are connected by a synchronous belt for synchronous transmission. This allows shaft A202 to rotate clockwise or counterclockwise, synchronously driving shaft B302 to rotate clockwise or counterclockwise. The rotation of shaft B302, in turn, causes the amplification placement rack 301 fixedly connected to its top to rotate as well. During the rotation of the amplification placement rack 301, each sample vial 206 inside the rack is evenly exposed to the environment inside the amplification chamber 3, ensuring that the amplification conditions for each sample are consistent, thereby improving the amplification efficiency. To improve the uniformity and stability of the nucleic acid amplification reaction; after the nucleic acid amplification reaction is completely finished, first loosen the knob 308 at the top of the screw 306, slide the sealing cover 304 upwards to open it, then rotate the baffle 305, and then, following the reverse steps of the previous sample transfer, use the auxiliary components to hold the amplified sample bottle by the clamping cylinder 404, and use the electric telescopic rod 403 to retract and the turntable B401 to rotate, so that the sample bottle is taken out from the amplification placement rack 301 and transferred to the designated sample collection area. Finally, the staff will perform subsequent detection and analysis operations on these amplified samples.
[0019] Although the present invention has been described above with reference to embodiments, various modifications can be made and components can be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the features in the embodiments disclosed in this invention can be combined with each other in any way. The lack of an exhaustive description of these combinations in this specification is merely for the sake of brevity and resource conservation. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.
Claims
1. An integrated nucleic acid extraction and amplification device, comprising an operating frame (1), wherein a support (2) is mounted on one side of the bottom of the operating frame (1), characterized in that: An amplification chamber (3) is installed on one side of the top of the operating frame (1); An auxiliary component is installed above the operating frame (1). The auxiliary component includes a mounting frame (4), and a turntable B (401) is rotatably connected to the top center of the mounting frame (4).
2. The integrated nucleic acid extraction and amplification device according to claim 1, characterized in that: The bottom of the bracket (2) is equipped with a motor A (201), and a rotating shaft A (202) is installed at the output end of the motor A (201).
3. The integrated nucleic acid extraction and amplification device according to claim 2, characterized in that: The outer wall of the rotating shaft A (202) is provided with a synchronous wheel A (203), and the top of the rotating shaft A (202) is equipped with a turntable A (204).
4. The integrated nucleic acid extraction and amplification device according to claim 3, characterized in that: The top of the turntable A (204) is equipped with a sample extraction rack (205), and the sample extraction rack (205) is slidably connected to the sample extraction bottles (206).
5. The integrated nucleic acid extraction and amplification device according to claim 1, characterized in that: An amplification placement rack (301) is rotatably connected inside the amplification chamber (3). A rotating shaft B (302) is installed at the bottom center of the amplification placement rack (301). A synchronous wheel B (303) is provided on the outer wall of the rotating shaft B (302). A sealing cover (304) is installed on the top of the amplification chamber (3). A baffle (305) is rotatably connected to one side of the top of the sealing cover (304).
6. The integrated nucleic acid extraction and amplification device according to claim 5, characterized in that: Screws (306) are installed on both sides of the amplification chamber (3), and fixing seats (307) are provided on both sides of the sealing cover (304). The interior of the fixing seat (307) is slidably connected to the outer wall of the screw (306), and a knob (308) is threadedly connected to the upper part of the outer wall of the screw (306).
7. The integrated nucleic acid extraction and amplification device according to claim 1, characterized in that: A motor B (402) is installed above the turntable B (401), and the output end of the motor B (402) is fixedly connected to the top center of the turntable B (401).
8. The integrated nucleic acid extraction and amplification device according to claim 7, characterized in that: An electric telescopic rod (403) is installed on one side of the top of the turntable B (401), and a clamping cylinder (404) is installed at the output end of the electric telescopic rod (403).