A nucleic acid amplification tube

Abstract

The utility model relates to medical equipment technical field discloses a nucleic acid amplification tube, including lower pipe body, upper pipe body, sealing membrane and puncture piece, the upper end of lower pipe body is open and forms injection port, the upper end of upper pipe body has liquid outlet, the lower end of upper pipe body is open and forms the communicating port, the lower end of upper pipe body is worn and is inserted in lower pipe body in injection port, upper pipe body and lower pipe body detachable connection and seal injection port, sealing membrane covers the communicating port, and puncture piece is located in lower pipe body, wherein, upper pipe body can move towards the side of lower pipe body, and puncture piece is used for puncturing sealing membrane, so that lower pipe body and upper pipe body are communicated, the nucleic acid amplification tube of the application does not need to open the cover and extract nucleic acid amplification solution after amplification like traditional amplification tube, can reduce aerosol pollution.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology, and more specifically, relates to a nucleic acid amplification tube. Background Technology

[0002] In nucleic acid amplification detection technology, the amplification tube is the core carrier for realizing the nucleic acid amplification reaction. Traditional amplification tubes consist of a tube body and a cap, with the cap connected to the tube body to form a closed containment cavity. In practical applications, nucleic acid amplification is carried out in the containment cavity. After the nucleic acid amplification is completed, the cap needs to be opened to extract the product from the tube body for subsequent detection. With this type of amplification tube that requires opening the cap to extract the product, the aerosols generated during the nucleic acid amplification process are very likely to spill out of the tube body into the environment, thus causing aerosol contamination. Utility Model Content

[0003] The main objective of this invention is to provide a nucleic acid amplification tube that can reduce aerosol contamination.

[0004] According to a first aspect of this utility model, a nucleic acid amplification tube is provided, comprising a lower tube body, an upper tube body, a sealing film, and a puncture device. The upper end of the lower tube body is open to form an injection port, the upper end of the upper tube body has an outlet nozzle, and the lower end of the upper tube body is open to form a communication port. The lower end of the upper tube body passes through the injection port and is inserted into the lower tube body. The upper tube body and the lower tube body are detachably connected and the injection port is sealed. The sealing film covers the communication port. The puncture device is disposed in the lower tube body. The upper tube body is movable toward the side where the lower tube body is located. The puncture device is used to puncture the sealing film to make the lower tube body and the upper tube body communicate.

[0005] In a specific embodiment of this utility model, the upper tube and the lower tube are detachably connected by an insertion method, and the outer peripheral surface of the upper tube is in contact with the inner peripheral surface of the lower tube.

[0006] In a specific embodiment of this utility model, the outer circumferential surface of the lower end of the upper tube body is provided with an external thread, and the inner circumferential surface of the upper end of the lower tube body is provided with an internal thread. The external thread is connected to the internal thread so that the upper tube body and the lower tube body can be detachably connected.

[0007] In a specific embodiment of this utility model, the nucleic acid amplification tube further includes a positioning rod, which includes a first rod body and a second rod body. The length direction of the first rod body extends radially along the upper tube body, and the first rod body is fixedly connected to the outer peripheral surface of the upper tube body. The length direction of the second rod body extends axially along the upper tube body. One end of the second rod body along its length direction is fixedly connected to the first rod body, and the other end contacts the outer edge of the upper end of the lower tube body. The second rod body and the upper tube body are spaced apart.

[0008] In a specific embodiment of this utility model, the second rod has a guide slope at one end away from the first rod along its length direction, and the guide slope contacts the outer edge of the upper end of the lower tube.

[0009] In a specific embodiment of this utility model, the lower tube body includes a conical section and a cylindrical section, the cylindrical section is disposed above the conical section, and the cylindrical section is connected to the larger end of the conical section, and the cylindrical section is detachably connected to the upper tube body.

[0010] In a specific embodiment of this utility model, the puncture component includes a fixing ring, a mounting plate, a support rod, a connecting column, and a puncture head. The fixing ring is positioned at the junction of the cylindrical segment and the conical segment. The mounting plate is located inside the fixing ring. The support rod connects the inner circumferential surface of the fixing ring and the outer surface of the mounting plate. There are multiple support rods, which are spaced apart around the center of the fixing ring. The length direction of the connecting column extends along the axial direction of the cylindrical segment. One end of the connecting column along its length direction is fixedly connected to the side of the mounting plate facing the sealing film, and the other end is fixedly connected to the puncture head. The puncture head is conical, and the smaller end of the puncture head faces the sealing film.

[0011] The diameter of the connecting post is smaller than the bottom diameter of the puncture head.

[0012] In a specific embodiment of this utility model, the puncture member further includes a limiting rod, which is fixedly connected to the side of the mounting plate facing the upper tube body. Along the radial direction of the cylindrical segment, the limiting rod is at least partially located outside the puncture head.

[0013] In a specific embodiment of this utility model, there are multiple limiting rods, which are spaced apart around the connecting column. Along the radial direction of the cylindrical segment, the end of the limiting rod near the connecting column is fixedly connected to the connecting column, and along the axial direction of the cylindrical segment, the limiting rod is spaced apart from the puncture head.

[0014] In a specific embodiment of this utility model, the outer peripheral surface of the upper tube body is also provided with anti-slip texture.

[0015] One of the above-described technical solutions of this utility model has at least one of the following advantages or beneficial effects:

[0016] This novel nucleic acid amplification tube, when amplifying nucleic acid, first separates the lower and upper tube bodies. Then, the nucleic acid sample, activator, and diluent are added to the lower tube body through the injection port. The lower tube body is then connected to the upper tube body to seal the injection port. Afterward, the nucleic acid amplification tube is placed in a metal bath for amplification. After amplification, the upper tube body is moved towards the side where the lower tube body is located to allow the puncture device to puncture the sealing membrane. At this point, the lower and upper tube bodies are connected. Then, the nucleic acid amplification tube is inverted and the outlet is inserted into the injection slot of the nucleic acid detection cartridge. The nucleic acid amplification solution flows into the nucleic acid detection cartridge for detection due to gravity. In this process, there is no need to open the cap and extract the nucleic acid amplification solution after amplification, as is the case with traditional amplification tubes, which can reduce aerosol contamination. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0018] Figure 1 This is a three-dimensional view of the nucleic acid amplification tube according to an embodiment of this utility model;

[0019] Figure 2 This is a cross-sectional view of the nucleic acid amplification tube according to an embodiment of this utility model;

[0020] Figure 3 This is an embodiment of the present utility model. Figure 2 A magnified view of A in the middle;

[0021] Figure 4 This is a perspective view of the lower tube body according to an embodiment of the present invention;

[0022] Figure 5 This is a perspective view of the puncture component according to an embodiment of the present invention;

[0023] Figure 6 This is an exploded view of a nucleic acid amplification tube according to another embodiment of this utility model.

[0024] In the diagram, 1. Lower tube body; 101. Injection port; 11. Conical section; 12. Cylindrical section; 2. Upper tube body; 201. Dispensing nozzle; 202. Connecting port; 3. Sealing film; 4. Puncture piece; 41. Fixing ring; 42. Mounting plate; 43. Support rod; 44. Connecting column; 45. Puncture head; 46. Limiting rod; 5. Positioning rod; 51. First rod body; 52. Second rod body; 5201. Guide slope; 100. Anti-slip texture. Detailed Implementation

[0025] The embodiments of this utility model are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0026] Reference Figures 1 to 5 As shown, a preferred embodiment of the present application provides a nucleic acid amplification tube, comprising a lower tube body 1, an upper tube body 2, a sealing film 3, and a puncture member 4. The upper end of the lower tube body 1 is open to form an injection port 101. The upper end of the upper tube body 2 has an outlet nozzle 201, and the lower end of the upper tube body 2 is open to form a connecting port 202. The lower end of the upper tube body 2 passes through the injection port 101 and is inserted into the lower tube body 1. The upper tube body 2 and the lower tube body 1 are detachably connected and the injection port 101 is sealed. The sealing film 3 covers the connecting port 202. The puncture member 4 is disposed inside the lower tube body 1. The upper tube body 2 is movable toward the side where the lower tube body 1 is located. The puncture member 4 is used to puncture the sealing film 3 to connect the lower tube body 1 and the upper tube body 2.

[0027] In practical applications, when amplifying nucleic acids, the lower tube 1 and upper tube 2 are first disassembled and separated. Then, the nucleic acid sample, activator, and diluent are added to the lower tube 1 through the injection port 101. The lower tube 1 is then connected to the upper tube 2 to seal the injection port 101. After that, the nucleic acid amplification tube is placed in a metal bath for amplification. After amplification, the upper tube 2 is moved toward the side where the lower tube 1 is located so that the puncture device 4 punctures the sealing membrane 3. At this time, the lower tube 1 and the upper tube 2 are connected. Then, the nucleic acid amplification tube is inverted and the outlet 201 is inserted into the injection slot of the nucleic acid detection card. The nucleic acid amplification solution flows into the nucleic acid detection card for detection due to gravity. In this process, there is no need to open the cap and extract the nucleic acid amplification solution after amplification, as is the case with traditional amplification tubes, which can reduce aerosol contamination.

[0028] In this embodiment, the outer circumferential surface of the lower end of the upper tube body 2 is provided with an external thread, and the inner circumferential surface of the upper end of the lower tube body 1 is provided with an internal thread. The external thread and the internal thread are connected to make the upper tube body 2 and the lower tube body 1 detachably connected. The structure is simple and can achieve the sealing of the injection port 101. On this basis, the upper tube body 2 can be moved toward the side where the lower tube body 1 is located by rotating the upper tube body 2, which is convenient to operate.

[0029] Furthermore, the outer circumferential surface of the upper tube body 2 is also provided with anti-slip texture 100. The anti-slip texture 100 is designed to facilitate the operator's operation of rotating the upper tube body 2 and prevent slippage.

[0030] In another embodiment, such as Figure 6As shown, the upper tube 2 and the lower tube 1 are detachably connected by a plug-in connection. The outer circumferential surface of the upper tube 2 contacts the inner circumferential surface of the lower tube 1. This structure enables the detachable connection between the upper tube 2 and the lower tube 1. The structure is simple and can achieve the sealing of the injection port 101. On this basis, the upper tube 2 can be moved towards the side where the lower tube 1 is located by pushing the upper tube 2, which is convenient to operate. Furthermore, the outer circumferential surface of the upper tube 2 can also be provided with anti-slip texture 100. The anti-slip texture 100 increases the contact area between the operator's hand and the upper tube 2, which can also prevent slippage.

[0031] In this embodiment, the nucleic acid amplification tube also includes a positioning rod 5, which includes a first rod body 51 and a second rod body 52. ​​The length direction of the first rod body 51 extends radially along the upper tube body 2 and is fixedly connected to the outer peripheral surface of the upper tube body 2. The length direction of the second rod body 52 extends axially along the upper tube body 2. One end of the second rod body 52 along its length direction is fixedly connected to the first rod body 51, and the other end contacts the outer edge of the upper end of the lower tube body 1. The second rod body 52 and the upper tube body 2 are spaced apart. Specifically, the function of the positioning rod 5 is to initially define the position of the upper tube body 2. In actual application, when the operator connects the upper tube body 2 and the lower tube body 1, the operator will feel resistance when the positioning rod 5 contacts the outer edge of the lower tube body 1. At this time, it indicates that the upper tube body 2 and the lower tube body 1 are installed in place, avoiding the operator's misoperation that causes the upper tube body 2 to be inserted into the lower tube body 1 to an excessive depth before nucleic acid amplification, thereby avoiding premature puncture of the sealing film 3.

[0032] When it is necessary to pierce the sealing film 3, the distance between the second rod 52 and the upper tube 2 can be manually widened so that the lower tube 1 can be inserted between the second rod 52 and the upper tube 2, ensuring that the upper tube 2 can move towards the side where the lower tube 1 is located, thereby enabling the piercing element 4 to pierce the sealing film 3. As a preferred embodiment of this application, the end of the second rod 52 away from the first rod 51 along its length direction has a guide slope 5201, which contacts the outer edge of the upper end of the lower tube 1. At this time, there is no need to manually widen the distance between the second rod 52 and the upper tube 2. The operator can directly drive the upper tube 2 towards the side where the lower tube 1 is located. The edge of the lower tube 1 will move relative to the upper tube 2 along the guide slope 5201 and then enter between the second rod 52 and the upper tube 2, thereby ensuring that the upper tube 2 can move smoothly and the operation is convenient.

[0033] In this embodiment, the lower tube 1 includes a conical segment 11 and a cylindrical segment 12. The cylindrical segment 12 is located above the conical segment 11 and is connected to the larger end of the conical segment 11. The cylindrical segment 12 is detachably connected to the upper tube 2. The cylindrical segment 12 has the aforementioned liquid injection port 101. With this structure, the conical segment 11 of the lower tube 1 can achieve a liquid aggregation effect, which is beneficial for nucleic acid amplification.

[0034] In this embodiment, the puncture component 4 includes a fixing ring 41, a mounting plate 42, a support rod 43, a connecting post 44, and a puncture head 45. The fixing ring 41 is positioned at the junction of the cylindrical segment 12 and the conical segment 11, meaning the outer diameter of the fixing ring 41 matches the inner diameter of the cylindrical segment 12. The mounting plate 42 is located inside the fixing ring 41. The support rod 43 connects the inner circumferential surface of the fixing ring 41 and the outer surface of the mounting plate 42. There are multiple support rods 43, spaced apart around the center of the fixing ring 41. For example, there are three support rods 43. The length direction of the connecting post 44 extends axially along the cylindrical segment 12. One end of the connecting post 44 is fixedly connected to the side of the mounting plate 42 facing the sealing film 3, and the other end is fixedly connected to the puncture head 45. The puncture head 45 is conical, with the smaller end of the puncture head 45 facing the sealing film 3. The diameter of the connecting post 44 is smaller than the bottom diameter of the puncture head 45, where the bottom diameter refers to the maximum diameter of the larger end of the puncture head 45. Specifically, this type of puncture component 4 has a simple structure and can be easily installed inside the lower tube 1 by simply placing it in place. In practical applications, when the upper tube 2 moves toward the side where the lower tube 1 is located, the puncture head 45 will puncture the sealing film 3, causing the sealing film 3 to form a connecting hole, thereby connecting the upper tube 2 and the lower tube 1. Since the diameter of the connecting post 44 is smaller than the bottom diameter of the puncture head 45, the diameter of the connecting hole formed by the sealing film 3 will also be larger than the diameter of the connecting post 44. Based on this, a liquid flow channel will be formed between the connecting post 44 and the inner wall of the connecting hole. The liquid flow channel allows the nucleic acid amplification solution to flow from the lower tube 1 into the upper tube 2, and then out from the outlet 201 of the upper tube 2.

[0035] In this embodiment, the sealing film 3 is an aluminum-plastic film, which is connected to the upper tube 2 by heat sealing. The sealing film 3 is circular, and the puncture head 45 is coaxially arranged with the sealing film 3. Based on this, when the puncture head 45 punctures the sealing film 3, the sealing film 3 will deform towards the side where the liquid outlet 201 is located under the force of the puncture head 45. Thus, the side of the sealing film 3 facing away from the liquid outlet 201 will form a flow guide slope, which is conducive to the nucleic acid amplification solution entering the upper tube 2 from the connecting hole.

[0036] In practical applications, since the nucleic acid amplification tube is inverted when discharging the nucleic acid amplification solution, and the puncture component 4 is placed inside the lower tube 1, in order to prevent the puncture component 4 from sliding down after the nucleic acid amplification tube is inverted, thereby blocking the connecting plate from closing the communication hole, the puncture component 4 further includes a limiting rod 46. The limiting rod 46 is fixedly connected to the side of the mounting plate 42 facing the upper tube 2. Along the radial direction of the cylindrical segment 12, the limiting rod 46 is at least partially located outside the puncture head 45. Along the axial direction of the cylindrical segment 12, the limiting rod 46 is spaced apart from the puncture head 45. Thus, even if the puncture component 4 slides down, the limiting rod 46 will first contact the sealing film 3, thereby preventing the puncture component 4 from sliding down further, avoiding the connecting plate from blocking the communication hole, and ensuring that the nucleic acid amplification solution can smoothly enter the upper tube 2.

[0037] Furthermore, there are multiple limiting rods 46, which are spaced apart around the connecting column 44. Along the radial direction of the cylindrical segment 12, one end of the limiting rod 46 near the connecting column 44 is fixedly connected to the connecting column 44. Thus, the limiting rod 46 can enhance the structural strength of the connecting column 44. For example, there are three limiting rods 46.

[0038] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A nucleic acid amplification tube, characterized in that, The device includes a lower tube (1), an upper tube (2), a sealing film (3), and a puncture device (4). The upper end of the lower tube (1) is open to form an injection port (101). The upper end of the upper tube (2) has an outlet (201). The lower end of the upper tube (2) is open to form a connecting port (202). The lower end of the upper tube (2) passes through the injection port (101) and is inserted into the lower tube (1). The upper tube (2) is detachably connected to the lower tube (1) and seals the injection port (101). The sealing film (3) covers the connecting port (202). The puncture device (4) is located inside the lower tube (1). The upper tube (2) can move toward the side where the lower tube (1) is located. The puncture device (4) is used to puncture the sealing film (3) so that the lower tube (1) and the upper tube (2) are connected.

2. The nucleic acid amplification tube according to claim 1, characterized in that, The upper tube (2) and the lower tube (1) are detachably connected by a plug-in connection, and the outer circumferential surface of the upper tube (2) is in contact with the inner circumferential surface of the lower tube (1).

3. The nucleic acid amplification tube according to claim 1, characterized in that, The outer circumferential surface of the lower end of the upper tube (2) is provided with an external thread, and the inner circumferential surface of the upper end of the lower tube (1) is provided with an internal thread. The external thread is connected to the internal thread so that the upper tube (2) and the lower tube (1) can be detachably connected.

4. The nucleic acid amplification tube according to any one of claims 2-3, characterized in that, The nucleic acid amplification tube also includes a positioning rod (5), which includes a first rod body (51) and a second rod body (52). The length direction of the first rod body (51) extends radially along the upper tube body (2). The first rod body (51) is fixedly connected to the outer peripheral surface of the upper tube body (2). The length direction of the second rod body (52) extends axially along the upper tube body (2). One end of the second rod body (52) along its length direction is fixedly connected to the first rod body (51), and the other end contacts the outer edge of the upper end of the lower tube body (1). The second rod body (52) and the upper tube body (2) are spaced apart.

5. The nucleic acid amplification tube according to claim 4, characterized in that, The second rod (52) has a guide slope (5201) at one end away from the first rod (51) along its length direction, and the guide slope (5201) contacts the outer edge of the upper end of the lower tube (1).

6. The nucleic acid amplification tube according to claim 4, characterized in that, The lower tube (1) includes a conical section (11) and a cylindrical section (12). The cylindrical section (12) is located above the conical section (11) and is connected to the larger end of the conical section (11). The cylindrical section (12) is detachably connected to the upper tube (2).

7. The nucleic acid amplification tube according to claim 6, characterized in that, The puncture component (4) includes a fixing ring (41), a mounting plate (42), a support rod (43), a connecting column (44), and a puncture head (45). The fixing ring (41) is positioned at the junction of the cylindrical section (12) and the conical section (11). The mounting plate (42) is located inside the fixing ring (41). The support rod (43) connects the inner circumferential surface of the fixing ring (41) and the outer surface of the mounting plate (42). The number of support rods (43) is multiple. One or more of the support rods (43) are spaced apart around the center of the fixing ring (41). The length direction of the connecting column (44) extends along the axial direction of the cylindrical segment (12). One end of the connecting column (44) along its length direction is fixedly connected to the side of the mounting plate (42) facing the sealing film (3), and the other end is fixedly connected to the puncture head (45). The puncture head (45) is conical, and the smaller end of the puncture head (45) faces the sealing film (3). The diameter of the connecting post (44) is smaller than the bottom diameter of the puncture head (45).

8. The nucleic acid amplification tube according to claim 7, characterized in that, The puncture component (4) also includes a limiting rod (46), which is fixedly connected to the mounting plate (42) on the side facing the upper tube (2). Along the radial direction of the cylindrical section (12), the limiting rod (46) is at least partially located outside the puncture head (45). Along the axial direction of the cylindrical section (12), the limiting rod (46) is spaced apart from the puncture head (45).

9. The nucleic acid amplification tube according to claim 8, characterized in that, There are multiple limiting rods (46), and the multiple limiting rods (46) are arranged at intervals around the connecting column (44). Along the radial direction of the cylindrical segment (12), the end of the limiting rod (46) near the connecting column (44) is fixedly connected to the connecting column (44).

10. The nucleic acid amplification tube according to claim 4, characterized in that, The outer circumferential surface of the upper tube (2) is also provided with anti-slip texture (100).

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