A glutamate dehydrogenase immobilized enzyme catalytic reaction device

Abstract

The utility model relates to the technical field of immobilized enzyme reaction, specifically disclose a kind of glutamate dehydrogenase immobilized enzyme catalytic reaction device, including shell, the inside fixed mounting of shell has bearing plate, the top of shell is fixedly installed with top plate, the bottom of top plate is fixedly connected with spoiler bar, the top of top plate is fixedly connected with feeding pipe and guide tube respectively, the inside rotation of shell is installed with reaction cylinder, the bottom of reaction cylinder is fixedly installed with fixed sleeve, the bottom of reaction cylinder is fixedly connected with discharge pipe, reaction liquid is put from connecting pipe, separation hole can filter the impurity in reaction liquid, spiral conveying blade rotation can push the impurity filtered to open and discharge, the impurity filtered is cleaned in time, second motor drives reaction cylinder to rotate slowly, spoiler bar is in fixed state, so that material is disturbed by relative thrust, avoid immobilized enzyme to be damaged by shear force.

Description

Technical Field

[0001] This utility model relates to the field of immobilized enzyme reaction technology, specifically to a glutamate dehydrogenase immobilized enzyme catalytic reaction device. Background Technology

[0002] Glutamate dehydrogenase is widely distributed in animal tissues such as liver, brain, kidney, and muscle, mainly located in the inner mitochondrial membrane. It is one of the key enzymes in the urea cycle. Immobilized enzyme technology fixes water-soluble enzymes onto an insoluble carrier, making them water-insoluble enzyme derivatives that can be used repeatedly and continuously. This technology solves the problems existing in the engineering application of enzymes and greatly improves the application value of enzymes.

[0003] In the prior art, such as the immobilized enzyme reaction device for continuous production disclosed in Chinese Patent Publication No. CN220376700U, there is a reaction vessel. A motor is installed in the middle of the top of the reaction vessel. The output end of the motor passes through the middle of the top of the reaction vessel and is fixedly connected to a rotating rod. Several stirring rods are fixedly connected to the surface of the rotating rod from top to bottom.

[0004] The aforementioned patent application filters the added materials by setting up a feeding component to prevent impurities from entering the reaction tank and affecting the quality. However, during long-term feeding, excessive impurities will clog the filter screen, requiring manual cleaning of the filter screen at regular intervals. Repeated operation can easily lead to worker fatigue and further reduce efficiency. In addition, the stirring rod in the aforementioned application is in direct contact with the immobilized enzyme particles when rotating. The shearing force during its rotation may damage the structure of the immobilized enzyme particles, thereby affecting the reaction quality of the immobilized enzyme. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a glutamate dehydrogenase immobilized enzyme catalytic reaction device, which solves the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a glutamate dehydrogenase immobilized enzyme catalytic reaction device, comprising a shell, a support plate fixedly installed inside the shell, a bearing fixedly installed at the center of the support plate, a ball bearing rotatably installed on the top of the bearing, a top plate fixedly installed on the top of the shell, a baffle rod fixedly connected to the bottom of the top plate, a feeding pipe and a guide cylinder fixedly connected to the top of the top plate respectively, a connecting pipe fixedly installed on the top of the guide cylinder, a first motor installed on one side of the guide cylinder, an installation shaft rotatably installed inside the guide cylinder, a reaction cylinder rotatably installed inside the shell, a fixing sleeve fixedly installed at the bottom of the reaction cylinder, a discharge pipe fixedly connected to the bottom of the reaction cylinder, a gear fixedly installed on the surface of the fixing sleeve, and a second motor fixedly installed at the bottom of the shell.

[0007] Preferably, the turbulence rod extends downward and is disposed inside the reaction cylinder, the bottom of the guide cylinder has an opening, and the top of the guide cylinder is inclined.

[0008] Preferably, the side of the mounting shaft is fixedly connected to the output end of the first motor, a spiral conveying blade is fixedly mounted on the surface of the mounting shaft, the surface of the spiral conveying blade is in contact with the inner wall of the guide cylinder, and a separation hole is provided on the side of the spiral conveying blade.

[0009] Preferably, the reaction cylinder is disposed on the top of the support plate and fits against the top of the ball, and the outer surface of the fixed sleeve is rotatably connected to the inner wall of the bearing.

[0010] Preferably, the bottom end of the discharge pipe extends to the bottom of the outer casing, and one end of the surface of the discharge pipe penetrates the interior of the fixing sleeve.

[0011] Preferably, a rotating rod is fixedly installed at the output end of the second motor, the top of the rotating rod is rotatably connected to the bottom of the bearing plate, and a gear is fixedly installed on the surface of the rotating rod.

[0012] Preferably, the first gear is disposed at the bottom of the support plate, and the side of the first gear meshes with the side of the second gear.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] 1. In this utility model, by tilting the feed cylinder, the reaction liquid is fed from the connecting pipe. The reaction liquid slides inside the feed cylinder due to its own gravity. The separation holes on the side of the spiral conveyor can filter impurities in the reaction liquid. The first motor controls the rotation of the mounting shaft and the spiral conveyor to push the filtered impurities to the opening for discharge. The filtered impurities can be cleaned in time to prevent them from clogging the feed cylinder. Furthermore, there is no need for manual cleaning of impurities, thereby improving production efficiency.

[0015] 2. In this utility model, through the meshing between gear one and gear two, when the second motor drives gear one and gear two to rotate, the fixed sleeve can drive the reaction cylinder to rotate slowly. At the same time, the reaction liquid and materials inside the reaction cylinder follow the rotation. The turbulence rod is fixed inside the reaction cylinder, so that the materials are disturbed by relative thrust. While stirring the materials, the immobilized enzyme is prevented from being damaged by shear force, thus extending the service life of the immobilized enzyme. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main three-dimensional structure of a glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of the outer shell of a glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model;

[0018] Figure 3 This is a three-dimensional structural diagram of the support plate of the glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model.

[0019] Figure 4 This is a schematic diagram of the bottom mounting structure of the reaction cylinder of the glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model;

[0020] Figure 5 This is a schematic diagram of the internal structure of the feed cylinder of a glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model;

[0021] Figure 6 This is an enlarged schematic diagram of section A of the glutamate dehydrogenase immobilized enzyme catalytic reaction device proposed in this utility model.

[0022] In the diagram: 1. Outer shell; 11. Support plate; 12. Bearing; 13. Ball bearing; 2. Top plate; 21. Baffle rod; 22. Feeding pipe; 23. Guide cylinder; 231. Opening; 24. Connecting pipe; 25. First motor; 26. Mounting shaft; 27. Screw conveyor blade; 271. Separation hole; 3. Reaction cylinder; 31. Fixed sleeve; 32. Discharge pipe; 33. Gear one; 4. Second motor; 41. Rotating rod; 42. Gear two. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Example 1

[0025] like Figures 1-6As shown, this utility model provides a technical solution: a glutamate dehydrogenase immobilized enzyme catalytic reaction device, including a shell 1, a support plate 11 fixedly installed inside the shell 1, a bearing 12 fixedly installed at the center of the support plate 11, a ball bearing 13 rotatably installed on the top of the bearing 12, a top plate 2 fixedly installed on the top of the shell 1, a baffle rod 21 fixedly connected to the bottom of the top plate 2, a feeding pipe 22 and a guide cylinder 23 fixedly connected to the top of the top plate 2, a connecting pipe 24 fixedly installed on the top of the guide cylinder 23, a first motor 25 installed on one side of the guide cylinder 23, an installation shaft 26 rotatably installed inside the guide cylinder 23, and a connecting pipe 24 fixedly installed inside the shell 1. There is a reaction cylinder 3, a fixed sleeve 31 is fixedly installed at the bottom of the reaction cylinder 3, a discharge pipe 32 is fixedly connected to the bottom of the reaction cylinder 3, a gear 33 is fixedly installed on the surface of the fixed sleeve 31, a second motor 4 is fixedly installed at the bottom of the outer shell 1, a baffle rod 21 extends downward and is set inside the reaction cylinder 3, an opening 231 is opened at the bottom of the guide cylinder 23, the top of the guide cylinder 23 is inclined, the side of the mounting shaft 26 is fixedly connected to the output end of the first motor 25, a spiral conveying blade 27 is fixedly installed on the surface of the mounting shaft 26, the surface of the spiral conveying blade 27 is in contact with the inner wall of the guide cylinder 23, and a separation hole 271 is opened on the side of the spiral conveying blade 27.

[0026] In this embodiment, the feeding pipe 22 and the connecting pipe 24 are used to feed and add solidified enzyme material and reaction solution, respectively. The bottom outlet of the feeding pipe 22 is located at the top of the reaction cylinder 3, allowing the material to flow directly into the reaction cylinder 3 for storage and reaction. The bottom end of the connecting pipe 24 is connected to the inside of the guide cylinder 23, allowing the fed reaction solution to flow inside the guide cylinder 23. Due to the inclined setting of the guide cylinder 23, the reaction solution slides down into the reaction cylinder 3 under its own gravity. The spiral conveyor blade 27 can block the reaction solution, preventing the reaction solution and impurities from flowing directly into the reaction cylinder 3. The separation hole 271 allows the reaction solution to slide downward. By installing a filter membrane inside the separation hole 271, impurities in the reaction solution can be filtered and separated. The first motor 25 controls the rotation of the mounting shaft 26. The rotation of the mounting shaft 26 and the spiral conveyor blade 27 can push the filtered impurities to the top of the guide cylinder 23. The opening 231 can discharge the impurities, preventing the filtered impurities from accumulating inside the guide cylinder 23 and causing blockage.

[0027] Example 2

[0028] like Figures 1-6As shown, the reaction cylinder 3 is set on the top of the support plate 11 and fits against the top of the ball bearing 13. The outer surface of the fixed sleeve 31 is rotatably connected to the inner wall of the bearing 12. The bottom end of the discharge pipe 32 extends to the bottom of the outer shell 1. One end of the surface of the discharge pipe 32 penetrates the interior of the fixed sleeve 31. The output end of the second motor 4 is fixedly installed with a rotating rod 41. The top of the rotating rod 41 is rotatably connected to the bottom of the support plate 11. The surface of the rotating rod 41 is fixedly installed with a gear 42. The gear 33 is set at the bottom of the support plate 11. The side of the gear 33 meshes with the side of the gear 42.

[0029] In this embodiment, the gear 33 mounted on the surface of the fixed sleeve 31 meshes with the gear 42, so that the reaction cylinder 3 can rotate when the second motor 4 drives the rotating rod 41 to rotate. The fixed sleeve 31 is installed inside the bearing 12 to reduce the wear of the fixed sleeve 31 when it rotates. The ball bearing 13 set on the top of the bearing plate 11 can reduce the contact area with the reaction cylinder 3 and reduce the friction, so that the reaction cylinder 3 can rotate with less effort. The material inside the reaction cylinder 3 is subjected to the centrifugal force of the reaction cylinder 3 and flows with the rotation. The turbulence rod 21 is fixed inside the reaction cylinder 3, so that the material is disturbed by the relative thrust, so that the reaction liquid and the material can carry out a catalytic reaction. At the same time, it can avoid the immobilized enzyme being damaged by excessive shear force when stirring the material. By installing a valve inside the discharge pipe 32 to control the opening and closing of the discharge pipe 32, the immobilized enzyme after the reaction can be discharged and collected.

[0030] Working principle: Solidified enzyme material and reaction solution are respectively fed into the feeding pipe 22 and the connecting pipe 24. The material flows directly into the reaction cylinder 3 for storage and reaction, while the reaction solution flows inside the guide cylinder 23. The reaction solution slides down into the reaction cylinder 3 through the separation hole 271. The filter membrane inside the separation hole 271 can filter and separate impurities in the reaction solution. The first motor 25 is started by an external power source to drive the rotation of the mounting shaft 26. The spiral conveying blades 27 on the surface of the mounting shaft 26 rotate and push the filtered impurities to the opening 231 for discharge, avoiding the accumulation of filtered impurities in the guide cylinder 23 and causing blockage. When the reaction solution and material are added into the reaction cylinder 3 together, the second motor 4 is started to drive the rotating rod 41 to rotate. The second gear 42 drives the first gear 33 to make the reaction cylinder 3 rotate. The material inside the reaction cylinder 3 is subjected to centrifugal force and flows with the rotation. The material is disturbed by the relative thrust of the turbulence rod 21, so that the reaction solution and material can carry out catalytic reaction. At the same time, it can avoid the immobilized enzyme being damaged by excessive shear force when stirring the material.

[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for immobilized glutamate dehydrogenase catalytic reaction, characterized in that: The system includes an outer shell (1), inside which a support plate (11) is fixedly installed. A bearing (12) is fixedly installed at the center of the support plate (11). A ball bearing (13) is rotatably installed on the top of the bearing (12). A top plate (2) is fixedly installed on the top of the outer shell (1). A baffle rod (21) is fixedly connected to the bottom of the top plate (2). A feeding pipe (22) and a guide cylinder (23) are fixedly connected to the top of the top plate (2). A connecting rod is fixedly installed on the top of the guide cylinder (23). The tube (24), a first motor (25) is installed on one side of the guide cylinder (23), an installation shaft (26) is rotatably installed inside the guide cylinder (23), a reaction cylinder (3) is rotatably installed inside the outer shell (1), a fixing sleeve (31) is fixedly installed at the bottom of the reaction cylinder (3), a discharge pipe (32) is fixedly connected to the bottom of the reaction cylinder (3), a gear (33) is fixedly installed on the surface of the fixing sleeve (31), and a second motor (4) is fixedly installed at the bottom of the outer shell (1).

2. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The turbulence rod (21) extends downward and is disposed inside the reaction cylinder (3). The bottom of the guide cylinder (23) has an opening (231), and the top of the guide cylinder (23) is inclined.

3. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The side of the mounting shaft (26) is fixedly connected to the output end of the first motor (25). A spiral conveying blade (27) is fixedly mounted on the surface of the mounting shaft (26). The surface of the spiral conveying blade (27) is in contact with the inner wall of the guide cylinder (23). A separation hole (271) is opened on the side of the spiral conveying blade (27).

4. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The reaction cylinder (3) is set on the top of the support plate (11) and fits against the top of the ball (13). The outer surface of the fixed sleeve (31) is rotatably connected to the inner wall of the bearing (12).

5. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The bottom end of the discharge pipe (32) extends to the bottom of the outer shell (1), and one end of the surface of the discharge pipe (32) penetrates the interior of the fixing sleeve (31).

6. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The output end of the second motor (4) is fixedly mounted with a rotating rod (41), the top of the rotating rod (41) is rotatably connected to the bottom of the bearing plate (11), and a gear (42) is fixedly mounted on the surface of the rotating rod (41).

7. The glutamate dehydrogenase immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The first gear (33) is disposed at the bottom of the support plate (11), and the side of the first gear (33) meshes with the side of the second gear (42).

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