A tumor organoid culture dish device

Abstract

The utility model discloses a kind of tumor organoid culture dish devices, it is related to petri dish field, including culture dish, the top outside of culture dish is docked with dish cover, the inner wall bottom end of culture dish is integrally formed with the protruding ring that protrudes upwards, the inside space of protruding ring is cell culture space, the top end of protruding ring is flush with the top end of culture dish, and the top end of protruding ring is open structure, one end of the outer wall of protruding ring is provided with liquid changing mechanism that is penetrated to the upper side of dish cover.The utility model is set by setting pipette mechanism, when replacing culture solution, dish cover does not need to be opened, just need to open the inlet of liquid changing pipe, with the advantage that small exposure area, easy to heat sterilization, to reduce the infection risk in tumor cell culture process.

Description

Technical Field

[0001] This utility model relates to the field of culture dishes, specifically a tumor organoid culture dish device. Background Technology

[0002] Culture dishes used for culturing tumor cells are generally ultra-low adsorption culture dishes, which can inhibit the adhesion of tumor cells and promote the aggregation of tumor cells to form organoids.

[0003] In existing technologies, culture medium containing consumable nutrients such as carbohydrates, amino acids, vitamins, and inorganic salts needs to be injected into the culture dish. During the culture process, the medium needs to be replaced regularly to maintain cell growth and metabolism. When replacing the existing culture dish, the lid needs to be completely removed, which exposes the cultured cells to the outside, resulting in a high risk of infection (such as bacterial or fungal infection). Utility Model Content

[0004] The purpose of this invention is to provide a tumor organoid culture dish device in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a tumor organoid culture dish device, comprising a culture dish, a dish lid being attached to the outer top of the culture dish, an upwardly protruding ring integrally formed at the bottom of the inner wall of the culture dish, the internal space of the protruding ring being a cell culture space, the top of the protruding ring being flush with the top of the culture dish, and the top of the protruding ring having an open structure, and a liquid exchange mechanism penetrating to the top of the dish lid being provided at one end of the outer wall of the protruding ring.

[0006] As a further embodiment of this utility model: the liquid changing mechanism includes a connection port at the bottom of the outer periphery of the protruding ring, the outer wall of the connection port is integrally formed with a liquid changing tube in an "L" shape, and the top plate of the dish cover is provided with a round hole for the vertical part of the liquid changing tube to pass through.

[0007] As a further embodiment of this utility model: the fluid changing mechanism further includes a threaded connecting cylinder that is threadedly connected to the upper part of the vertical tube of the fluid changing pipe, and the top of the threaded connecting cylinder is integrally formed with a handle.

[0008] As a further embodiment of this utility model: the inner wall of the threaded connecting cylinder is formed with an internal thread, and the upper part of the vertical tube of the fluid exchange pipe is provided with an external thread, and the internal thread and the external thread are engaged.

[0009] Compared with the prior art, the beneficial effects of this utility model are: By setting up a pipetting mechanism, when changing the culture medium, there is no need to open the dish lid; only the inlet of the medium-changing tube needs to be opened. This has the advantages of small exposure area and easy heat sterilization, thereby reducing the risk of infection during tumor cell culture. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the internal structure of the present invention; Figure 3 For the present utility model Figure 2 Enlarged view of a portion of point A in the middle.

[0011] In the diagram: 1. Petri dish; 2. Dish lid; 3. Threaded connector; 4. Handle; 5. Protruding ring; 6. Connecting port; 7. Liquid exchange tube. Detailed Implementation

[0012] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0013] Please see Figures 1-3 In this embodiment of the present invention, a tumor organoid culture dish device includes a culture dish 1, a dish cover 2 connected to the outer top of the culture dish 1, an upwardly protruding ring 5 integrally formed at the bottom of the inner wall of the culture dish 1, the internal space of the protruding ring 5 being a cell culture space, the top of the protruding ring 5 being flush with the top of the culture dish 1, and the top of the protruding ring 5 having an open structure, and a liquid exchange mechanism penetrating to the top of the dish cover 2 is provided at one end of the outer wall of the protruding ring 5.

[0014] In this embodiment: the inner wall of the culture dish 1 and the inner wall of the protruding ring 5 are covalently bonded to react with a special bottom-adsorbing polymer to form a uniform and firm ultrathin polymer coating, which prevents cells from adhering to the wall and is conducive to the aggregation of tumor cells to form organoids. For example, a hydrophilic polymer (polyethylene oxide) is attached to the surface of the culture dish 1 and the protruding ring 5 by means of a tool. These polymer molecular chains will form a hydration layer in water, preventing cells from adhering to the wall. During cancer cell culture, when changing the medium, the opening of the medium changing mechanism is opened, and then the culture dish 1 is tilted towards the medium changing mechanism. The culture medium is then aspirated using a pipette, and after aspiration, new culture medium is injected using a pipette. In this way, the air inside the culture dish 1 can be completely exposed to the outside. The small area of ​​exposure can be prevented from being contaminated by heating the small area of ​​exposure (generally, the exposed area is heated near the flame of an alcohol lamp).

[0015] Please refer to the diagram carefully. Figure 2 and Figure 3 The fluid changing mechanism includes a connection port 6 located at the bottom of the outer periphery of the protruding ring 5. The outer wall of the connection port 6 is integrally formed with an "L"-shaped fluid changing tube 7. The top plate of the lid 2 has a round hole for the vertical tube of the fluid changing tube 7 to pass through. The fluid changing mechanism also includes a threaded connecting cylinder 3 threadedly connected to the upper part of the vertical tube of the fluid changing tube 7. The top of the threaded connecting cylinder 3 is integrally formed with a handle 4.

[0016] In this embodiment: When performing pipetting, first turn the handle 4 to open the threaded connecting sleeve 3. By tilting the culture dish 1 towards the liquid exchange tube 7, the culture medium in the culture dish 1 enters the liquid exchange tube 7 under gravity. At this time, insert the tip of the pipette into the liquid exchange tube 7 and aspirate to remove the culture medium that needs to be replaced. After the aspiration is complete, use the pipette to inject the new culture medium into the inner wall of the protruding ring 5 through the liquid exchange tube 7. Finally, thread the threaded connecting sleeve 3 to the top of the liquid exchange tube 7. By designing this liquid exchange mechanism, the exposed area can be reduced, and it is convenient to avoid contamination problems by heating. It should be noted that a cavity is formed between the outer wall of the protruding ring 5 and the circumferential inner wall of the culture dish 1, and the liquid exchange tube 7 is located in the cavity, which can protect the liquid exchange tube 7 and prevent damage caused by collision during movement and placement.

[0017] Please refer to this carefully. Figure 3 The inner wall of the threaded connecting cylinder 3 is formed with an internal thread, and the upper part of the vertical pipe of the fluid exchange pipe 7 is provided with an external thread, and the internal thread and the external thread mesh with each other.

[0018] In this embodiment: the threaded design enables the installation and removal of the threaded connecting cylinder 3, and the threaded connecting cylinder 3 in the installed state can limit the top of the lid 2, preventing the lid 2 from falling due to instability during the handling process.

[0019] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A tumor organoid culture dish device comprising a culture dish (1), characterized in that, The top outer side of the culture dish (1) is butt jointed with a dish cover (2), the inner wall bottom end of the culture dish (1) is integrally formed with a protruding ring (5) protruding upward, the inner space of the protruding ring (5) is a cell culture space, the top end of the protruding ring (5) is flush with the top end of the culture dish (1), and the top end of the protruding ring (5) is in an open structure, and one end of the outer wall of the protruding ring (5) is provided with a liquid changing mechanism penetrating above the dish cover (2).

2. The tumor organoid culture dish device of claim 1, wherein, The liquid changing mechanism comprises a connecting port (6) opened at the outermost bottom end of the protruding ring (5), the outer wall of the connecting port (6) is integrally formed with an "L" type structure liquid changing pipe (7), and the top plate of the dish cover (2) is provided with a circular hole for the vertical pipe part of the liquid changing pipe (7) to pass through.

3. The tumor organoid culture dish device of claim 2, wherein, The liquid changing mechanism further comprises a threaded connection cylinder (3) screwing on the outer wall of the vertical pipe part of the liquid changing pipe (7), and the top end of the threaded connection cylinder (3) is integrally formed with a handle (4).

4. The tumor organoid culture dish device of claim 3, wherein, The inner wall of the threaded connection cylinder (3) is formed with an internal thread, the outer wall of the vertical pipe part of the liquid changing pipe (7) is provided with an external thread, and the internal thread and the external thread are engaged.

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