Embryo culture device

Abstract

The utility model discloses an embryo culture device, including culture storehouse, illumination device, and culture storehouse includes shell and storehouse cover, and the red light illumination device is fixed at the culture storehouse outside, and the storehouse cover and / or shell are equipped with the illumination window that supplies light to pass through, and illumination device includes illumination light source, illumination even lens, and illumination even lens is used to make light even and gather it into a uniform light spot, and the shape of light spot is adapted to the shape of culture dish, and the area of light spot is not less than the cross -sectional area of culture area of culture dish. The utility model discloses an embryo culture device utilizes the illumination device to pass through the illumination window and carries out the illumination to the embryo located in the culture storehouse, has realized the demand of carrying out the light illumination to the embryo under the state of keeping the environment of embryo stable, utilizes the illumination device to make light form the uniform light spot to the uniform illumination to the embryo after, to guarantee that each part of embryo can get the development of equality.

Description

Technical Field

[0001] This utility model relates to the field of embryo culture, and in particular to an embryo culture device. Background Technology

[0002] In recent years, studies have found that red light irradiation can enhance cellular metabolic activity, increase intracellular energy levels, provide more energy support for oocyte maturation, reduce oxidative stress damage during embryonic development, stimulate embryonic cell proliferation and differentiation, and accelerate embryonic development. Therefore, red light irradiation technology has gradually gained attention in embryonic development research. However, in specific application studies, it has been found that red light irradiation in the field of embryonic development faces challenges such as the inability to precisely control light intensity according to the embryonic development process, the need to irradiate embryos outside the culture environment, and the inability to monitor embryonic development in real time after red light irradiation. These issues hinder the large-scale clinical application of red light irradiation in embryonic development. Therefore, solving these technical bottlenecks to enable large-scale clinical application of red light irradiation in embryonic development is a problem that urgently needs to be addressed in this field. Utility Model Content

[0003] To address the problems existing in the prior art, this utility model discloses an embryo culture device.

[0004] An embryo culture device includes a culture chamber and an irradiation device. The culture chamber includes an outer shell and a cover. The irradiation device is fixed outside the culture chamber. The cover and / or the outer shell are provided with irradiation windows for light to pass through.

[0005] The irradiation device includes an irradiation light source and an irradiation uniform lens. The irradiation uniform lens is used to make the light uniform and converge it into a uniform light spot. The shape of the light spot is adapted to the shape of the culture dish, and the area of ​​the light spot is not less than the cross-sectional area of ​​the culture area of ​​the culture dish.

[0006] Specifically, the irradiation device is fixed outside the culture chamber, and the position between the irradiation device and the culture chamber is relatively fixed. The light emitted by the irradiation light source is formed into a uniform light spot through the uniform irradiation lens. The light spot passes through the irradiation window and shines into the culture chamber. By positioning the culture dish in a position that matches the light spot, the embryo can be irradiated with light. With this structure, the embryo is always in a stable environment in the culture chamber and does not need to be removed from the culture environment, thus achieving the dual requirements of light irradiation and environmental control for the embryo.

[0007] In addition, the structure with a light spot area not less than the cross-sectional area of ​​the culture dish and the uniform light spot obtained by using a uniform irradiation lens can ensure that all parts of the culture dish are uniformly irradiated.

[0008] More preferably, the light emitted by the irradiation light source is red light or other light that affects embryonic development.

[0009] Preferably, the irradiation device is tilted, and the plane containing the irradiation light source and the uniform irradiation lens forms an angle of less than 90° with the plane containing the irradiation window.

[0010] Specifically, by tilting the irradiation device so that the plane containing the irradiation light source, the uniform irradiation lens, and the irradiation window forms an angle of less than 90°, the distance between the irradiation light source, the uniform irradiation lens, and the irradiation window can be effectively increased, thereby obtaining a larger light spot. This makes the embryo culture device more compact while ensuring that the cross-sectional area of ​​the light spot is not less than the cross-sectional area of ​​the culture dish.

[0011] Preferably, the culture chamber further includes a turntable with multiple compartments for placing culture dishes. By rotating the turntable, each compartment can be aligned with the position of the irradiation window.

[0012] Specifically, the structure of the turntable allows multiple culture dishes to be placed in one culture chamber. The position of the culture dishes can be switched by rotating the turntable, and they can be rotated to the irradiation window for light irradiation according to the specific needs of each culture dish.

[0013] Preferably, it also includes a photographic imaging device, which is located outside the culture chamber. The culture chamber is provided with a viewing window for the photographic imaging device to take pictures of the embryos inside the culture chamber. The position of the viewing window does not coincide with the position of the irradiation window, and the viewing window is sealed to the culture chamber.

[0014] Specifically, during the embryo culture process, it is necessary to take pictures to observe its developmental status. Taking pictures of the embryos before and after light exposure allows us to determine its developmental status and understand its response to light exposure, thus enabling us to adjust the exposure parameters accordingly.

[0015] Preferably, the photographing and imaging device includes a camera for taking pictures, a moving mechanism for moving the camera position, a focusing mechanism for focusing, and an imaging optical path.

[0016] Preferably, the system also includes a petri dish label recognition device, which is used to identify petri dish labels and send the label information to the control module.

[0017] Specifically, this structure enables information-based management of petri dish data, effectively reducing the probability of data errors and mismatches.

[0018] Preferably, the control module calculates the light irradiation parameters of the corresponding culture dish based on the label information of each culture dish, and controls the irradiation device to irradiate each culture dish with light according to the set parameter values.

[0019] Specifically, the control module sets targeted light irradiation parameters for each culture dish based on the label information, thereby achieving high-precision control of the light irradiation dose for the embryos in each culture dish.

[0020] Preferably, it also includes a temperature control system for controlling the temperature inside the culture chamber.

[0021] Specifically, the embryo requires precise temperature control during its culture and development, so a structure with a temperature control system is appropriate.

[0022] Preferably, it also includes a gas concentration control system, which is used to control the concentration of each gas in the culture chamber.

[0023] Specifically, embryos are very sensitive to changes in their gaseous environment during the culture and development process, so it is appropriate to set up a gas concentration control system.

[0024] Preferably, the outer shell is sealed to the bin cover, and the irradiation window is sealed to the outer shell or bin cover.

[0025] Specifically, this structure can effectively ensure the airtightness of the culture chamber environment, thereby enabling precise control over the environment in which the embryos are located.

[0026] Compared with the prior art, the advantages of this utility model are:

[0027] An irradiation device is used to irradiate embryos located in the culture chamber through an irradiation window, achieving the requirement of irradiating embryos while maintaining a stable environment. The irradiation device forms a uniform light spot to irradiate the embryos evenly, ensuring that all parts of the embryo develop equally. An imaging device is used to photograph the embryos to confirm their developmental status. A culture dish label identification device is used to achieve data management of the culture dish parameters. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the embryo culture device disclosed in this utility model;

[0029] Figure 2 This is a cross-sectional view of the culture chamber structure of the embryo culture device disclosed in this utility model;

[0030] Figure 3 This is a schematic diagram of the internal structure of the embryo culture device disclosed in this utility model. Detailed Implementation

[0031] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0032] like Figure 1-3 As shown, the embryo culture device includes a culture chamber and an irradiation device 30. The culture chamber includes an outer shell 10 and a chamber cover 11. The irradiation device 30 is fixed outside the culture chamber. The chamber cover 11 and / or the outer shell 10 are provided with an irradiation window 20 for light to pass through.

[0033] The irradiation device 30 includes an irradiation light source 31 and an irradiation uniform lens 32. The irradiation uniform lens 32 is used to make the light uniform and converge it into a uniform light spot. The shape of the light spot is adapted to the shape of the culture dish, and the area of ​​the light spot is not less than the cross-sectional area of ​​the culture area of ​​the culture dish.

[0034] The irradiation device 30 is fixed outside the culture chamber, and the position between the irradiation device 30 and the culture chamber is relatively fixed. The light emitted by the irradiation light source 31 forms a uniform light spot through the uniform irradiation lens 32. The light spot passes through the irradiation window 20 and shines into the culture chamber. The embryo can be irradiated by placing the culture dish in a position that matches the light spot. With this structure, the embryo is always in a stable environment in the culture chamber and does not need to be removed from the culture environment, thus achieving the dual requirements of light irradiation and environmental control for the embryo.

[0035] In addition, the structure with a light spot area not less than the cross-sectional area of ​​the culture dish and the uniform light spot obtained by using the uniform irradiation lens 32 can ensure that all parts of the culture dish are uniformly irradiated.

[0036] The light emitted by the illumination source 31 is red light or other light that affects embryonic development.

[0037] The irradiation device 30 is tilted, and the plane containing the irradiation light source 31 and the uniform irradiation lens 32 forms an angle of less than 90° with the plane containing the irradiation window 20.

[0038] By tilting the irradiation device 30 so that the plane containing the irradiation light source 31, the uniform irradiation lens 32, and the irradiation window 20 forms an angle of less than 90°, the distance between the irradiation light source 31, the uniform irradiation lens 32, and the irradiation window 20 can be effectively increased, thereby obtaining a larger light spot. This makes the embryo culture device structure more compact while ensuring that the cross-sectional area of ​​the light spot is not less than the cross-sectional area of ​​the culture dish.

[0039] The culture chamber also includes a turntable 12, which has multiple compartments for placing culture dishes. By rotating the turntable 12, each compartment can be aligned with the position of the irradiation window 20.

[0040] The structure of the turntable 12 allows multiple culture dishes to be placed in a culture chamber. The position of the culture dishes can be switched by rotating the turntable 12. The culture dishes can be rotated to the irradiation window 20 for light irradiation according to the specific needs of each culture dish.

[0041] It also includes a photographic imaging device 40, which is located outside the culture chamber. The culture chamber has a viewing window 41 for the photographic imaging device 40 to take pictures of the embryos inside the culture chamber. The position of the viewing window 41 does not coincide with the position of the irradiation window 20, and the viewing window 41 is sealed to the culture chamber.

[0042] During the embryo culture process, it is necessary to take pictures to observe its developmental status. Taking pictures of the embryos before and after light exposure allows us to determine its developmental status and understand its response to light exposure, which in turn allows us to adjust the exposure parameters.

[0043] The photographic imaging device 40 includes a camera for taking pictures, a moving mechanism for moving the camera position, a focusing mechanism for focusing, and an imaging optical path.

[0044] It also includes a petri dish label recognition device 50, which is used to recognize petri dish labels and send the label information to the control module.

[0045] This structure enables information-based management of petri dish data, effectively reducing data errors and mismatches.

[0046] The control module calculates the light irradiation parameters for each culture dish based on the label information of each culture dish, and controls the irradiation device 30 to irradiate each culture dish with light according to the set parameter values.

[0047] The control module sets specific light irradiation parameters for each culture dish based on the label information, thereby achieving high-precision control of the light irradiation dose for the embryos in each culture dish.

[0048] It also includes a temperature control system, which is used to control the temperature inside the culture chamber.

[0049] Precise temperature control is required during embryo culture and development, so a structure with a temperature control system is appropriate.

[0050] It also includes a gas concentration control system, which is used to control the concentration of various gases in the culture chamber.

[0051] Embryos are highly sensitive to changes in their gaseous environment during culture and development, so a structure with a gas concentration control system is appropriate.

[0052] The outer casing 10 is sealed to the cover 11, and the irradiation window 20 is sealed to the outer casing 10 or the cover 11.

[0053] This structure can effectively ensure the airtightness of the culture chamber environment, thereby allowing for precise control of the environment in which the embryos are located.

Claims

1. An embryo culture device comprising a culture chamber, an irradiation device, characterized by, The culture chamber includes an outer shell and a cover, the irradiation device is fixed outside the culture chamber, and the cover and / or the outer shell are provided with irradiation windows for light to pass through; The irradiation device includes an irradiation light source and an irradiation uniform lens. The irradiation uniform lens is used to make the light uniform and converge it into a uniform light spot. The shape of the light spot is adapted to the shape of the culture dish, and the area of ​​the light spot is not less than the cross-sectional area of ​​the culture area of ​​the culture dish.

2. The embryo culture apparatus according to claim 1, characterized in that, The irradiation device is tilted, and the plane containing the irradiation light source and the uniform irradiation lens forms an angle of less than 90° with the plane containing the irradiation window.

3. The embryo culture apparatus according to claim 1, characterized in that, The culture chamber also includes a turntable with multiple compartments for placing culture dishes. By rotating the turntable, each compartment can be aligned with the position of the irradiation window.

4. The embryo culture apparatus according to claim 1, characterized in that, It also includes a photographic imaging device, which is located outside the culture chamber. The culture chamber has a viewing window for the photographic imaging device to take pictures of the embryos inside the culture chamber. The position of the viewing window does not coincide with the position of the irradiation window, and the viewing window is sealed to the culture chamber.

5. The embryo culture apparatus according to claim 4, characterized in that, The photographic imaging device includes a camera for taking pictures, a moving mechanism for moving the camera position, a focusing mechanism for focusing, and an imaging optical path.

6. The embryo culture apparatus according to claim 1, characterized in that, It also includes a petri dish label recognition device, which is used to identify petri dish labels and send the label information to the control module.

7. The embryo culture apparatus according to claim 6, characterized in that, The control module calculates the light irradiation parameters for each culture dish based on the label information of each culture dish, and controls the irradiation device to irradiate each culture dish with light according to the set parameter values.

8. The embryo culture apparatus according to claim 1, characterized in that, It also includes a temperature control system for controlling the temperature inside the culture chamber.

9. The embryo culture apparatus according to claim 1, characterized in that, It also includes a gas concentration control system, which is used to control the concentration of each gas in the culture chamber.

10. The embryo culture apparatus according to claim 1, characterized in that, The outer shell is sealed to the bin cover, and the irradiation window is sealed to the outer shell or bin cover.

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