A device for dehydration of cell-crawling sheets

Abstract

The utility model relates to cell culture technical field, concretely relates to a device for cell climbing piece dehydration, including dehydration box and dehydration frame in dehydration box, dehydration frame includes square bottom frame, and the bottom frame four corners are equipped with the support leg upwards respectively, and four support legs top are equipped with square top frame, still include two first baffle, two first baffle parallelly equipped in the corresponding support leg of dehydration frame's left and right sides, still include the partition unit that sets up along the front -rear direction evenly in dehydration frame, and each group partition unit includes two in the dehydration frame left and right opposite partition, and the dehydration interlayer that forms and cell climbing piece adaptation between two adjacent pairs of partition unit, still include dehydration pouch net, dehydration pouch net set up in the overall below that array partition unit and dehydration interlayer form, the utility model discloses can carry out dehydration treatment to multiple cell climbing pieces simultaneously, and the processing is efficient and convenient, and each cell climbing piece is independently soaked, guarantees each cell climbing piece and dehydrating agent reaction fully, and dehydrates fully efficient.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a device for dehydrating cells on a cell slide. Background Technology

[0002] Cell climbing slides, also known as cell climbing slides, refer to the process of immersing a glass slide in cell culture medium and allowing cells to grow on the slide. They are mainly used in histology, immunohistochemistry, frozen sections, cell smears, in situ hybridization, etc.

[0003] In cell culture and related experiments, dehydration of cell slides (such as slides or glass slides) is often necessary, especially for microscopic observation or cell staining. The main reasons for dehydration include: 1. Fixation and staining preparation: Many cell fixation and staining processes require a relatively dry environment to ensure that dyes or reagents can effectively penetrate the cells and intracellular structures. If too much water remains on the cell surface, it may affect dye penetration and binding with cellular components, thus affecting the uniformity and clarity of staining. Dehydration ensures clearer and more effective staining. 2. Enhanced observation: In microscopic observation, some water on glass slides or slides can interfere with transmitted light, thus reducing the observation effect. Dehydration improves the visualization of cells and their structures, helping scientists to make more accurate observations and analyses. 3. Reduced cell movement: Cells may move in liquid culture media, affecting cell positioning and fixation. In experiments requiring the observation of specific cell characteristics, dehydration reduces cell movement, thus maintaining the relative position of cells and enhancing experimental consistency. 4. Promoted adhesion: In some experimental designs, the effectiveness of cell adhesion to slides may be affected by water content. Pre-dehydration promotes cell adhesion to surfaces, helping to ensure cells do not detach during experiments. 5. Gradient treatment in the dehydration step: Typically, the concentration of ethanol or other solvents is gradually increased during dehydration to reduce damage caused by cell dehydration. This stepwise treatment helps improve the preservation quality of cell structures.

[0004] Existing cell slide dehydration methods require soaking the cell slides in a dehydrating agent. During dehydration, it is necessary to ensure that each slide is relatively independent so that it can be fully soaked and react fully with the dehydrating agent. This invention provides a device for dehydrating cell slides that can simultaneously dehydrate cells from multiple slides while ensuring that each slide is relatively independent and that dehydration is thorough. Utility Model Content

[0005] This invention provides a device for dehydrating cells on multiple cell slides simultaneously, while ensuring that each slide is relatively independent and that dehydration is thorough.

[0006] To achieve the above objectives, the technical solution of this utility model is as follows: A device for dehydrating cell slides is provided, comprising a dehydration box and a dehydration rack placed inside the dehydration box. The dehydration rack includes a square base frame, with legs extending upwards at the four corners of the base frame, and a square top frame at the top of the four legs. It also includes two first baffles, which are parallel to each other on the corresponding legs on the left and right sides of the dehydration rack. Furthermore, it includes a series of partition units evenly arranged along the front-back direction within the dehydration rack. Each partition unit includes two opposing partition strips within the dehydration rack, with the top of the partition strips connected to the inner side of the top frame and the bottom connected to the inner side of the first baffles. Adjacent pairs of partition units form a dehydration interlayer adapted to the cell slide. It also includes a dehydration net, which is positioned below the overall structure formed by the series of partition units and the dehydration interlayer. The top of the dehydration box is provided with a lid.

[0007] Furthermore, two side ears are symmetrically provided in the middle of the front and rear sides of the top frame, and each side ear is provided with a directional mark.

[0008] Furthermore, the two directional markings on the two side ears are both arrows pointing in the same direction.

[0009] Furthermore, a partition is connected between the two partition strips of each partition unit.

[0010] Furthermore, the aforementioned partitions have a mesh structure.

[0011] Furthermore, the connection method of the partition and the partition strip is as follows: two opposite partition strips are provided with connecting grooves with a horizontal cross section of T, and two connecting pieces with a horizontal cross section of T are provided on both sides of the partition corresponding to the two connecting grooves. The partition is sleeved in the connecting groove through the connecting pieces.

[0012] Furthermore, the distance between the two inner sides of the top frame is 100mm, the height of the spacer is 100mm, and the width of the dehydration interlayer is 3-4mm.

[0013] Furthermore, it also includes a handle, which is in the shape of an inverted U, with its two lower ends connected to the top center of the left and right sides of the top frame, respectively.

[0014] Furthermore, it also includes two second baffles, which are respectively disposed between the two first baffles and the top frame.

[0015] Furthermore, the dehydration box has a downward-facing slot on the inner top of the box, and the bottom of the box lid has a downward-facing locking block corresponding to the slot. When the box lid is placed on the dehydration box, the locking block is locked in the slot.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] This invention discloses a device for dehydrating cell slides, comprising a dehydration box and a dehydration rack. The dehydration rack contains dehydration chambers formed by partition units, and a dehydration net is provided at the bottom of the partition units and the dehydration chambers to support the cell slides. During dehydration, multiple cell slides are placed in the dehydration chambers, and the dehydration rack is placed in the dehydration box containing a dehydrating agent for soaking. This device can simultaneously dehydrate multiple cell slides, offering efficient and convenient processing. Furthermore, the independent soaking of each cell slide ensures thorough reaction with the dehydrating agent, resulting in complete and efficient dehydration. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure of a device for dehydrating cell slides according to the present invention.

[0020] Figure 2 This is a schematic diagram of the dehydration rack of this utility model (without the dehydration net and partition).

[0021] Figure 3 This is a schematic diagram of the dehydration rack of this utility model (without partitions).

[0022] Figure 4 This is a schematic diagram of the overall structure of the dehydration rack of this utility model.

[0023] Figure 5 This is a schematic diagram of the structure for placing cell spreaders between the dehydration interlayers formed by the spacers and segments in this invention.

[0024] Figure 6 This is a schematic diagram of the connection between the partition and the spacer in Embodiment 4 of this utility model.

[0025] Figure 7 In this utility model Figure 6 Enlarged view of point A in the image.

[0026] Figure 8 This is a cross-sectional view of the connection between the lid and the dehydration box in Embodiment 8 of this utility model.

[0027] The components are as follows: 1. Dehydration box; 2. Dehydration rack; 21. Base frame; 22. Support leg; 23. Top frame; 24. First baffle; 25. Spacer; 26. Dehydration interlayer; 27. Dehydration net; 3. Box lid; 4. Side ear; 5. Orientation marker; 6. Divider; 7. Connecting groove; 8. Connector; 9. Handle; 10. Second baffle; 11. Bayonet; 12. Locking block; 13. Cell climbing slide. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] The directional terms "front", "rear", "left", "right" and other directional terms used in this utility model are only used to describe the accompanying drawings and are not intended to limit the actual use of the device. In actual use, the orientation of the device shall be determined by the actual usage.

[0030] The device for dehydrating cell spreaders of this invention is mainly used for dehydrating cells from circular cell spreaders.

[0031] Example 1

[0032] This embodiment provides a device for dehydrating cell smears, such as... Figure 1 As shown, the device includes a dehydration box 1 and a dehydration rack 2 placed inside the dehydration box 1. The dehydration rack 2 includes a square base frame 21, with support legs 22 at the four corners of the base frame 21, and a square top frame 23 on the top of the four support legs 22. It also includes two first baffles 24, which are parallel to each other on the corresponding support legs 22 on the left and right sides of the dehydration rack 2. Furthermore, it includes a series of partition units evenly arranged along the front-to-back direction inside the dehydration rack 2, such as... Figure 2 As shown, each partition unit includes two opposing partition strips 25 within the dehydration rack 2. The top of the partition strips 25 is connected to the inner side of the top frame 23, and the bottom is connected to the inner side of the first baffle 24. A dehydration interlayer 26 adapted to the cell crawling sheet 13 is formed between adjacent pairs of partition units; it also includes a dehydration net 27, such as... Figure 3 As shown, the dehydration net 27 is located below the overall structure formed by the array partition unit and the dehydration interlayer 26; the top of the dehydration box 1 is provided with a box cover 3.

[0033] This embodiment includes a dehydration box 1 and a dehydration rack 2. The dehydration rack 2 has a dehydration interlayer 26 formed by various partition units. A dehydration net 27 is provided at the bottom of the partition units and the dehydration interlayer 26 to support the cell spreaders 13. During dehydration, multiple cell spreaders 13 are placed in the dehydration interlayer 26, and the dehydration rack 2 is placed in the dehydration box 1 containing a dehydrating agent for soaking. This allows for the simultaneous dehydration of multiple cell spreaders 13, making the process efficient and convenient. Furthermore, the independent soaking of each cell spreader 13 ensures that each cell spreader 13 fully reacts with the dehydrating agent, resulting in thorough and efficient dehydration.

[0034] Example 2

[0035] In this embodiment, two side ears 4 are symmetrically provided in the middle of the front and rear sides of the top frame 23, and each side ear 4 is provided with a directional mark 5.

[0036] Preferably, the two directional markers 5 on the two side ears 4 are both arrows pointing in the same direction.

[0037] The rest is the same as in Example 1.

[0038] In this embodiment, the side ear 4 allows for convenient and stable movement of the dehydration rack 2, while the orientation marker 5 indicates the orientation, facilitating the identification of cell slides 13 placed in different positions and aiding in subsequent observation of the cell slides 13. In actual use, when placing the cell slides 13, the direction indicated by the arrow can be defined as front or back to determine which end of the dehydration rack 2 is the front and which is the back, using the orientation marker 5 to indicate the position of the cell slides 13.

[0039] Example 3

[0040] In this embodiment, a partition 6 connects the two partition strips 25 of each partition unit.

[0041] like Figure 4 and 5 As shown, preferably, the segment 6 has a mesh structure.

[0042] The rest is the same as in Example 1.

[0043] In this embodiment, the partition 6 can support the cell climbing sheet 13 when a smaller diameter cell climbing sheet 13 is placed. Since the partition strip 25 is mainly used to support the cell climbing sheet 13 with a larger diameter, when the diameter of the placed cell climbing sheet 13 is small, its two ends cannot reach the position of the partition strip 25 on both sides, so it needs to be supported by the partition 6. The partition 6 in this embodiment makes the dehydration rack 2 of this utility model adaptable to the dehydration of cell climbing sheets 13 with different diameters, which is highly flexible, efficient and convenient to use.

[0044] Example 4

[0045] The connection method of the partition 6 and the partition strip 25 in this embodiment is as follows: Figure 6 and 7 As shown, two opposite partition bars 25 are provided with T-shaped horizontal connecting grooves 7, and two T-shaped horizontal connecting pieces 8 are provided on both sides of the partition 6 corresponding to the two connecting grooves 7. The partition 6 is sleeved in the connecting grooves 7 through the connecting pieces 8.

[0046] The rest is the same as in Example 3.

[0047] In this embodiment, the connection method between the partition 6 and the corresponding partition strip 25 makes the disassembly and installation of the partition 6 convenient. Corresponding to Embodiment 3, this invention enables the dehydration rack 2 to adapt to the dehydration of cell climbing sheets 13 with different diameters, and has high flexibility of use.

[0048] Example 5

[0049] In this embodiment, the distance between the left and right inner sides of the top frame 23 is 100mm, the height of the spacer 25 is 100mm, and the width of the dehydration interlayer 26 is 3-4mm.

[0050] The rest is the same as in Example 1.

[0051] Example 6

[0052] This embodiment also includes a handle 9, which is in the shape of an inverted U, and its two lower ends are respectively connected to the top middle of the left and right sides of the top frame 23.

[0053] The rest is the same as in Example 1.

[0054] The handle 9 in this embodiment makes it easy to move the dehydration rack 2 by hand.

[0055] Example 7

[0056] This embodiment also includes two second baffles 10, such as Figure 4 and 5 As shown, the two second baffles 10 are respectively located between the two first baffles 24 and the top frame 23.

[0057] The rest is the same as in Example 1.

[0058] In this embodiment, the second baffle 10 is preferably located at the lower middle part of the distance between the first baffle 24 and the top frame 23, which can provide further support for the cell climbing sheet 13 from the side.

[0059] Example 8

[0060] In this embodiment, the dehydration box 1 has a downward-facing latch 11 on the inner top side, such as... Figure 8 As shown, the bottom of the lid 3 is provided with a locking block 12 corresponding to the slot 11. When the lid 3 is placed on the dehydration box 1, the locking block 12 is locked in the slot 11.

[0061] The rest is the same as in Example 1.

[0062] The bayonet 11 and the locking block 12 are set to limit the lid 3 when the lid 3 is put on the dehydration box 1, so as to ensure the connection stability of the lid 3 and the dehydration box 1 in the horizontal direction.

[0063] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the concept and scope of the present utility model. Without departing from the design concept of the present utility model, all modifications and improvements made by those skilled in the art to the technical solutions of the present utility model should fall within the protection scope of the present utility model. The technical content for which protection is sought in the present utility model has been fully recorded in the technical requirements.

Claims

1. A device for dehydrating cell smears, characterized in that: The device includes a dehydration box and a dehydration rack placed inside the dehydration box. The dehydration rack includes a square base frame with four upward-facing legs at the four corners of the base frame and a square top frame on top of the four legs. It also includes two first baffles, which are parallel to each other on the corresponding legs on the left and right sides of the dehydration rack. Furthermore, it includes a series of partition units evenly arranged along the front-to-back direction inside the dehydration rack. Each partition unit includes two opposing partition strips inside the dehydration rack. The top of the partition strips connects to the inner side of the top frame, and the bottom connects to the inner side of the first baffles. Adjacent pairs of partition units form a dehydration interlayer adapted to cell spread. It also includes a dehydration net, which is located below the overall structure formed by the partition units and the dehydration interlayer. The top of the dehydration box has a lid.

2. The device for dehydrating cell smears according to claim 1, characterized in that: The top frame has two symmetrical side ears in the middle of the front and rear sides, and each side ear has a directional mark.

3. The device for dehydrating cell smears according to claim 2, characterized in that: Both directional markers on the two side ears are arrows pointing in the same direction.

4. The device for dehydrating cell smears according to claim 1, characterized in that: Each partition unit has a partition section connecting the two partition strips.

5. The apparatus for dehydrating cell smears according to claim 4, characterized in that: The aforementioned partition has a mesh structure.

6. The apparatus for dehydrating cell smears according to claim 4, characterized in that: The connection method of the partition and the partition strip is as follows: two opposite partition strips are provided with connecting grooves with a horizontal cross section of T, and two connecting pieces with a horizontal cross section of T are provided on both sides of the partition corresponding to the two connecting grooves. The partition is sleeved in the connecting groove through the connecting pieces.

7. The apparatus for dehydrating cell smears according to claim 1, characterized in that: The distance between the two inner sides of the top frame is 100mm, the height of the spacer is 100mm, and the width of the dehydration interlayer is 3-4mm.

8. The apparatus for dehydrating cell smears according to claim 1, characterized in that: It also includes a handle, which is in the shape of an inverted U and has its two lower ends connected to the top center of the left and right sides of the top frame, respectively.

9. The apparatus for dehydrating cell smears according to claim 1, characterized in that: It also includes two second baffles, which are respectively disposed between the two first baffles and the top frame.

10. The apparatus for dehydrating cell spreaders according to claim 1, characterized in that: The dehydration box has a downward-facing slot on the top inner side, and the box cover has a downward-facing locking block at the bottom corresponding to the slot. When the box cover is placed on the dehydration box, the locking block is locked in the slot.

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