A multifunctional tissue washing device
Patent Information
- Application Number
- CN202522182008.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-15
- Publication Date
- 2026-09-15
- Estimated Expiration
- 2035-10-15
AI Technical Summary
[0003]在免疫组织化实验过程中，组织的洗涤是其中的关键步骤，其一般以浸泡法为主，冲洗法为辅的方式，进行组织的洗涤处理，具体为在一抗和二抗孵育后，先用洗瓶快速冲洗掉玻片表面的大部分液体，再放入装有新鲜PBST的缸中进行浸泡洗涤，在这过程中往往需要使用多种装置，与玻片的反复拆卸安装，且各种装置的操作往往不同，降低了组织物的洗涤速率
该改进型多功能组织洗涤装置使用时，通过流动的洗涤液完成玻片上组织物的冲洗操作，并完成外箱内洗涤液的添加，在洗涤液漫过玻片后，可直接进行组织物的浸泡洗涤操作，同时驱动组织物外周侧的洗涤液持续流动，以加速残留试剂扩散，直至组织物的洗涤完成，通过一个装置即可完成组织物的冲洗和浸泡洗涤操作，装置自动化程度高，且不需要玻片反复拆装操作，提升了组织物的洗涤速率。
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Figure CN224758193U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of tissue washing devices, and more particularly to a multifunctional tissue washing device. Background Technology
[0002] Immunohistochemistry is an experimental technique that utilizes the principle of specific binding between antigens and antibodies. Through a chemical reaction, a chromogenic agent labeled with antibodies is made to develop color, thereby locating, identifying, and relatively quantifying specific antigens, usually proteins or peptides, within tissues or cells.
[0003] In immunohistochemistry experiments, tissue washing is a crucial step. It is generally carried out by immersion as the main method and rinsing as a supplementary method. Specifically, after incubation with primary and secondary antibodies, most of the liquid on the surface of the slide is quickly rinsed off with a wash bottle, and then the slide is placed in a container with fresh PBST for immersion and washing. This process often requires the use of multiple devices and repeated disassembly and assembly of the slides. Moreover, the operation of each device is often different, which reduces the washing rate of the tissue. Utility Model Content
[0004] Therefore, the technical problem to be solved by this utility model is to provide a multifunctional tissue washing device that can complete the rinsing and soaking washing of tissues with one device. The device has a high degree of automation and does not require repeated disassembly and assembly of glass slides, thereby improving the washing rate of tissues.
[0005] To solve the above problems, this utility model provides a multifunctional tissue washing device, including: an outer box, a partition fixedly installed at the bottom inside the outer box, and a circular hole opened at the center of the partition; a constraint ring fixedly installed in the top opening of the outer box; and a water pump fixedly installed on one side of the outer box. The first opening of the electrically controlled three-way valve is fixedly inserted into the outer casing, and the second opening of the electrically controlled three-way valve is connected to the bottom opening of the water pump through a connecting pipe. The drain pipe is fixedly installed inside the constraint ring, and the inlet of the drain pipe is fixedly installed through the constraint ring and the outer box wall and connected to the top opening of the water pump. Several outlets of the drain pipe are vertically downward. The sealing mechanism is located inside the side opening of the outer casing and is used to seal the gap between the glass slide and the side opening of the outer casing.
[0006] Preferably, the inner bottom wall of the outer casing is sloped, and an electrically controlled valve is provided below the electrically controlled three-way valve. The end of the electrically controlled valve near the outer casing extends to the bottommost side of the inner bottom wall of the outer casing and is fixedly connected to the outer casing.
[0007] Preferably, the partition is an arc-shaped plate that curves outward from the center, and a one-way valve is fixedly installed inside the circular hole.
[0008] Preferably, a pressure limiting valve is fixedly installed in several outlets of the drain pipe, and a mesh plate is provided above the partition, with the outer peripheral wall of the mesh plate fixedly connected to the inner wall of the outer casing.
[0009] Preferably, the bottom side of the constraint ring is fixedly connected to the mesh plate, and a filter plate is fixedly installed in the top opening of the constraint ring by a snap fastener.
[0010] Preferably, the sealing mechanism includes a soft pad with a U-shaped cross-section, which is fixedly installed on the inner wall of the side opening of the outer box. A constraint groove is provided in the box wall on the other side of the outer box. A push plate is slidably installed in the constraint groove, and the top side of the push plate is fixedly connected to the inside of the constraint groove by a number of springs. A soft plate is fixedly installed on the bottom side of the push plate, and the bottom side of the soft plate is inserted into the soft pad.
[0011] Preferably, a plurality of guide grooves are provided at equal intervals on the inner wall of the constraint groove, and a guide rod is slidably installed in the guide groove, with the bottom end of the guide rod being fixedly connected to the push plate.
[0012] This utility model has the following beneficial effects: This improved multifunctional tissue washing device uses a flowing washing solution to rinse tissues on glass slides and adds washing solution to the outer chamber. After the washing solution overflows the glass slide, the tissues can be directly immersed and washed. At the same time, the washing solution on the outer periphery of the tissues is continuously driven to accelerate the diffusion of residual reagents until the tissue washing is complete. The rinsing and immersion washing operations of tissues can be completed with a single device. The device has a high degree of automation and does not require repeated disassembly and reassembly of glass slides, thus improving the tissue washing rate. Attached Figure Description
[0013] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0014] Figure 1 This is a perspective view of the overall structure of this utility model; Figure 2 This utility model Figure 1 A 3D view of the inner and outer boxes after rotating 90 degrees counterclockwise; Figure 3 This is a front view of the internal structure of the outer casing of this utility model; Figure 4 This utility model Figure 3 Enlarged view of the structure at point A in the middle; Figure 5 This is a perspective view of the soft pad and soft board of this utility model.
[0015] In the diagram: 1. Outer casing; 2. Partition plate; 3. Circular hole; 4. Constraint ring; 5. Water pump; 6. Electrically controlled three-way valve; 7. Drain pipe; 8. Water outlet; 9. Sealing mechanism; 91. Soft pad; 92. Constraint groove; 93. Push plate; 94. Spring; 95. Soft plate; 96. Guide groove; 97. Guide rod; 10. Pressure limiting valve; 11. Electrically controlled valve; 12. Check valve; 13. Mesh plate; 14. Filter plate. Detailed Implementation
[0016] To make the technical solution and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0017] Example: Refer to Figure 1 , Figure 2 and Figure 3 The present invention discloses a multifunctional tissue washing device, comprising: an outer box 1, a partition 2 fixedly installed at the bottom inside the outer box 1, and a circular hole 3 opened at the center of the partition 2; a constraint ring 4 fixedly installed in the top opening of the outer box 1; and a water pump 5 fixedly installed on one side of the outer box 1. The first opening of the electrically controlled three-way valve 6 is fixedly inserted into the outer casing 1, and the second opening of the electrically controlled three-way valve 6 is connected to the bottom opening of the water pump 5 through a connecting pipe. The drain pipe 7 is fixedly installed inside the constraint ring 4, and the inlet of the drain pipe 7 is fixedly installed through the constraint ring 4 and the box wall of the outer box 1 and connected to the top opening of the water pump 5. Several outlets of the drain pipe 7 are vertically downward. The sealing mechanism 9 is located inside the side opening of the outer casing 1 and is used to seal the gap between the glass slide and the side opening of the outer casing 1.
[0018] In this embodiment, when the improved multifunctional tissue washing device is used, the washing liquid in the external storage tank is drawn out or the washing liquid in the outer box 1 is circulated through the cooperation of the water pump 5, the electrically controlled three-way valve 6 and the drain pipe 7. The washing liquid is used to complete the rinsing operation of the tissue on the slide through the flowing washing liquid. The waste liquid is blocked by the partition 2. After the washing liquid overflows the slide, the tissue can be directly soaked and washed. At the same time, the washing liquid on the outer periphery of the tissue is continuously driven to accelerate the diffusion of residual reagents until the tissue washing is completed. The rinsing and soaking washing operations of the tissue can be completed by one device. The device has a high degree of automation and does not require repeated disassembly and assembly of the slide, thus improving the tissue washing rate. (Please refer to) Figure 1The front side of the outer box 1 is fixedly equipped with a control panel and a control host for overall control of the device. When installing the glass slide, the sealing mechanism 9 is used to release the sealing state of the side opening of the outer box 1, and then the glass slide is directly inserted into the outer box 1 through the side opening of the outer box 1. Subsequently, the sealing mechanism 9 is used to close the gap between the glass slide and the inner wall of the side opening of the outer box 1. After the glass slide is installed, water pump 5 starts, continuously drawing washing liquid from the external storage tank via the electrically controlled three-way valve 6. (Please refer to...) Figure 2 As shown, the bottom three openings of the electrically controlled three-way valve 6 are connected to the storage tank through the water pump 5. The washing liquid drawn by the water pump 5 is directly injected into the drain pipe 7 and discharged from several outlets 8 of the drain pipe 7 (i.e., multiple locations on the top side of the tissue). The washing liquid discharged from the drain pipe 7 impacts the tissue to perform the tissue rinsing operation. Subsequently, due to the constraint and limitation of the constraint ring 4, the washing liquid slides to both sides of the slide and drips onto the partition 2 and passes through the round hole 3 to the bottom of the partition 2 located inside the outer box 1 for storage. Once the amount of washing solution stored in the outer box 1 reaches the threshold, the electrically controlled three-way valve 6 is activated, connecting the bottom opening of the air pump to the inside of the outer box 1. At this time, the water pump 5 continuously injects the washing solution from the bottom of the slide back into the outer box 1 to drive the washing solution around the tissue to flow continuously, accelerating the diffusion of residual reagents until the tissue soaking and washing operation is completed.
[0019] In a further preferred embodiment of this utility model, such as Figure 2 and Figure 3 As shown, the inner bottom wall of the outer box 1 is sloped, and an electric control valve 11 is provided below the electric control three-way valve 6. The end of the electric control valve 11 near the outer box 1 extends to the bottom of the inner bottom wall of the outer box 1 and is fixedly connected to the outer box 1. In this embodiment (please refer to...), Figure 2 As shown, the other end of the electrically controlled valve 11 is connected to a drain pipe. After the glass slide washing operation is completed, the electrically controlled valve 11 is opened, and the washing liquid in the outer box 1 flows out of the outer box 1 through the electrically controlled valve 11. The waste washing liquid is then guided to the corresponding location for discharge through the drain pipe (the corresponding location is the waste washing liquid discharge location such as water treatment equipment or wastewater storage equipment).
[0020] In a further preferred embodiment of this utility model, such as Figure 3 As shown, the partition 2 is an arc-shaped plate that curves outward from the center, and a one-way valve 12 is fixedly installed in the round hole 3. In this embodiment, the arc-shaped plate of the partition 2 allows the washing liquid falling on the partition 2 to flow towards the round hole 3, and pass through the one-way valve 12 to flow over the partition 2 and into the bottom storage inside the outer box 1. The one-way valve 12 can prevent the backflow of dirty washing liquid below the partition 2.
[0021] In a further preferred embodiment of this utility model, such as Figure 2 and Figure 3 As shown, pressure relief valves 10 are fixedly installed in several outlets of the drain pipe 7, and a mesh plate 13 is provided above the partition plate 2, with the outer peripheral wall of the mesh plate 13 fixedly connected to the inner wall of the outer box 1. In this embodiment, please refer to Figure 2 and Figure 3 As shown, the screen plate 13 is designed to support the glass slide from the bottom side without affecting the washing liquid on the glass slide from falling onto the partition plate 2. After the washing liquid is injected into the drain pipe 7, the drain pipe 7 will first be filled with washing liquid due to the obstruction of the pressure limiting valve 10. Then, due to the pressure difference between the two ends of the pressure limiting valve 10, the washing liquid in the drain pipe 7 will pass through the pressure limiting valve 10 and be discharged from the drain pipe 7, so as to avoid excessive washing liquid discharge from a single outlet 8, which would affect the rinsing of tissues.
[0022] In a further preferred embodiment of this utility model, such as Figure 1 As shown, the bottom side of the constraint ring 4 is fixedly connected to the mesh plate 13, and the filter plate 14 is fixedly installed in the top opening of the constraint ring 4 by a snap fastener. In this embodiment, the filter plate 14 can block dust in the outside air from entering the outer box 1, so as to prevent a large amount of dust from being brought into the outer box 1 when the outside gas flows into the outer box 1 as the washing liquid inside the outer box 1 decreases.
[0023] In a further preferred embodiment of this utility model, such as Figure 1 , Figure 3 and Figure 4 As shown, the sealing mechanism 9 includes a soft pad 91 with a U-shaped cross-section, which is fixedly installed on the inner wall of the side opening of the outer box 1. A constraint groove 92 is opened in the box wall on the other side of the outer box 1. A push plate 93 is slidably installed in the constraint groove 92, and the top side of the push plate 93 is fixedly connected to the inside of the constraint groove 92 by several springs 94. A soft plate 95 is fixedly installed on the bottom side of the push plate 93, and the bottom side of the soft plate 95 is inserted into the soft pad 91. In this embodiment, when installing the glass slide, the operator pushes the push plate 93 upward to separate the soft plate 95 from the soft pad 91, and then moves the glass slide so that it passes through the gap between the soft plate 95 and the soft pad 91. After the glass slide is inserted into the outer box 1, it slides on the mesh plate 13 until the glass slide abuts against the inner wall of the outer box 1. Then the push plate 93 is released. Due to the push of the spring 94 on the push plate 93, the soft plate 95 moves down and presses against the glass slide. At this time, due to the mutual abutment between the soft plate 95 and the soft pad 91 and the glass slide, and the mutual abutment between the soft plates 95, the side opening of the outer box 1 is closed, and the washing liquid inside the outer box 1 will not flow out of the outer box 1 from the side opening of the outer box 1.
[0024] In a further preferred embodiment of this utility model, such as Figure 3 and Figure 4 As shown, a number of guide grooves 96 are equally spaced on the inner wall of the constraint groove 92. A guide rod 97 is slidably installed in the guide groove 96, and the bottom end of the guide rod 97 is fixedly connected to the push plate 93. In this embodiment, the guide rod 97 and the guide groove 96 are designed to constrain the movement trajectory of the push plate 93 by the mutual contact between the guide rod 97 and the guide groove 96, so that the push plate 93 slides in the guide groove 96 along a linear up-down trajectory, thereby avoiding bending deformation of the top of the soft plate 95 and affecting the sealing effect of the opening on the side of the outer box 1.
[0025] Working principle: When using this improved multifunctional tissue washing device, the operator pushes the push plate 93 upward, and then inserts the glass slide into the outer box 1 through the side opening of the outer box 1. Then, the push plate 93 is released, and the soft pad 91, soft plate 95 and glass slide cooperate to seal the side opening of the outer box 1, and the installation of the glass slide is completed. The device is started via the control panel. The water pump 5 first draws the washing liquid from the storage tank and completes the rinsing of the tissue through the drain pipe 7. After the amount of washing liquid stored in the outer box 1 reaches the threshold, the water pump 5 continues to draw the washing liquid from the bottom side of the mesh plate 13. Then, the washing liquid is continuously driven to circulate from the outside to the inside through the drain pipe 7, so as to drive the washing liquid on the outer periphery to flow continuously until the soaking and washing operation of the tissue is completed. (The rinsing intensity of the washing liquid or the flow rate of the washing liquid on the outer periphery of the tissue can be adjusted by adjusting the power of the water pump 5.)
[0026] The embodiments described herein are preferred embodiments of this utility model and are not intended to limit the scope of protection of this utility model. Therefore, all equivalent changes made to the structure, shape, and principle of this utility model should be included within the scope of protection of this utility model.
Claims
1. A multifunctional tissue washing device, characterized in that, include: The outer box (1) has a partition (2) fixedly installed at the bottom inside, and a round hole (3) is opened at the center of the partition (2). A constraint ring (4) is fixedly installed in the top opening of the outer box (1), and a water pump (5) is fixedly installed on one side of the outer box (1). The first opening of the electrically controlled three-way valve (6) is fixedly inserted into the outer casing (1), and the second opening of the electrically controlled three-way valve (6) is connected to the bottom opening of the water pump (5) through a connecting pipe. The drain pipe (7) is fixedly installed inside the constraint ring (4), and the inlet of the drain pipe (7) is fixedly installed through the constraint ring (4) and the box wall of the outer box (1) and connected to the top opening of the water pump (5). Several outlets of the drain pipe (7) are vertically downward. The sealing mechanism (9) is located inside the side opening of the outer box (1) and is used to seal the gap between the glass slide and the side opening of the outer box (1).
2. The multifunctional tissue washing device according to claim 1, characterized in that: The inner bottom wall of the outer box (1) is sloped, and an electric control valve (11) is provided below the electric control three-way valve (6). The end of the electric control valve (11) near the outer box (1) extends to the bottom of the inner bottom wall of the outer box (1) and is fixedly connected to the outer box (1).
3. The multifunctional tissue washing device according to claim 2, characterized in that: The partition (2) is an arc-shaped plate that curves outward from the center, and a one-way valve (12) is fixedly installed in the round hole (3).
4. The multifunctional tissue washing device according to claim 3, characterized in that: Pressure limiting valves (10) are fixedly installed in several outlets of the drain pipe (7), and a mesh plate (13) is provided above the partition (2), and the outer peripheral wall of the mesh plate (13) is fixedly connected to the inner wall of the outer box (1).
5. A multifunctional tissue washing device according to claim 4, characterized in that: The bottom side of the constraint ring (4) is fixedly connected to the mesh plate (13), and the filter plate (14) is fixedly installed in the top opening of the constraint ring (4) by a snap fastener.
6. A multifunctional tissue washing device according to claim 5, characterized in that: The sealing mechanism (9) includes a soft pad (91) with a U-shaped cross-section, which is fixedly installed on the inner wall of the side opening of the outer box (1). A constraint groove (92) is provided in the box wall on the other side of the outer box (1). A push plate (93) is slidably installed in the constraint groove (92), and the top side of the push plate (93) is fixedly connected to the inner side of the constraint groove (92) by several springs (94). A soft plate (95) is fixedly installed on the bottom side of the push plate (93), and the bottom side of the soft plate (95) is inserted into the soft pad (91).
7. A multifunctional tissue washing device according to claim 6, characterized in that: The inner wall of the constraint groove (92) is provided with a number of guide grooves (96) at equal intervals. A guide rod (97) is slidably installed in the guide groove (96), and the bottom end of the guide rod (97) is fixedly connected to the push plate (93).