A micro area reflection interference spectrum detection system

Abstract

The utility model discloses a kind of micro area reflection interference spectroscopy detection systems, comprising: halogen lamp light source, for generating incident light;First prism, second prism, for controlling incident and reflected light path;Objective, aperture is configured to control condensing range, realize micro area detection;Mirror, for reflecting the transmission light of second prism;Eyepiece, for receiving the light reflected by mirror;Spectrometer, for collecting and analyzing optical signal;Displacement stage, along XYZ three directions movement, for accurate positioning and dynamic measurement;Flow cell, for carrying interference film sample;The utility model realizes micro area positioning detection by micrometer level displacement stage, can carry out selected area dynamic detection to sample.

Description

Technical Field

[0001] This invention relates to the field of molecular detection technology, and in particular to a micro-area reflection interferometry detection system. Background Technology

[0002] Biosensors, as an important carrier of modern detection technology, have been applied in many fields such as disease diagnosis, environmental monitoring, and drug screening. Traditional labeling analysis techniques have problems such as complex labeling processes, potential interference with biomolecular activity, and the need for additional labeling reagents. Label-free biosensors achieve analysis and detection by directly detecting changes in physical parameters (such as mass and refractive index) caused by biomolecular interactions, and have the following significant advantages: (1) no labeling step is required, reducing interference with biological activity; (2) easy to miniaturize the device; (3) supports real-time dynamic monitoring; and (4) has higher system stability.

[0003] Advances in thin-film technology have provided an ideal platform for biosensing. Thin-film sensing technology based on the principle of optical interference can achieve high-precision and sensitive real-time detection by detecting changes in the optical properties of the thin-film interface caused by the binding of biomolecules. Existing technologies all use ordered porous nanofilms as the interference sensing medium and achieve biomolecule detection based on the principle of reflection interference spectral changes. However, using conventional fiber optic spectrometers to directly acquire the average spectral signal of the macroscopic region cannot achieve micro-area localization detection, and the intrusion of stray light during signal acquisition leads to low data accuracy. Utility Model Content

[0004] Purpose of the utility model: The purpose of this utility model is to provide a micro-area reflection interferometric spectroscopy detection system to solve the problems of low accuracy of acquired data due to the inability to achieve micro-area positioning detection and the mixing of stray light during signal acquisition.

[0005] Technical Solution: The present invention discloses a micro-area reflection interferometric spectroscopy detection system, comprising: a halogen lamp light source for generating incident light; a first prism and a second prism for controlling the optical path and splitting the reflected light into two beams; an objective lens equipped with an aperture to control the focusing range and achieve micro-area detection; a reflector for reflecting the transmitted light from the second prism; an eyepiece for receiving the light reflected by the reflector; a spectrometer for acquiring and analyzing optical signals; a displacement stage that moves along the X, Y, and Z directions for precise positioning and dynamic measurement; and a flow cell for holding the interferometric film sample.

[0006] Furthermore, the system also includes a computer interface for connecting the spectrometer and camera to enable automatic data acquisition, storage, and analysis.

[0007] Furthermore, the system is divided into a signal light path and an indicator light path; the indicator light source of the indicator light path comes from the spectrometer and is output to the prism through an optical fiber; the indicator light path is used to assist in signal acquisition.

[0008] Furthermore, the second prism is used to split the reflected light from the flow cell into two parts: one part is reflected to the fiber optic input of the spectrometer, and the other part is transmitted through a mirror to the eyepiece or a connected camera.

[0009] Furthermore, the spectrometer includes a CCD detector for receiving optical signals transmitted through optical fibers and outputting the data to a computer for real-time processing and analysis.

[0010] Furthermore, the objective lens aperture can be adjusted to focus light down to the micrometer scale, thereby optimizing spatial resolution.

[0011] Furthermore, the XYZ three-dimensional movement accuracy of the displacement stage is at the micrometer level, which, combined with the controllable position of the flow cell, enables real-time dynamic measurement of biomolecular interactions.

[0012] Furthermore, the optical path design is based on an inverted microscope architecture, including a halogen lamp light source that is reflected to a flow cell by a first prism, and the reflected light returning from the flow cell is split by a second prism to optimize the optical signal path.

[0013] Furthermore, the flow cell is configured to be detachable to support ordered porous nanostructured thin films as interference sensing media, supporting the detection of label-free biomolecules.

[0014] Furthermore, the positions of the first prism, the second prism, and the reflector are adjustable to optimize the incident angle and the reflection angle.

[0015] Beneficial effects: Compared with the prior art, the present invention has the following advantages: Based on the inverted microscopic optical path structure and the adjustable aperture objective lens, the present invention can focus to the micrometer scale region, reduce the influence of stray light, and significantly improve the signal-to-noise ratio and resolution; at the same time, the micrometer-level displacement stage realizes micro-area positioning, which can perform selected area dynamic detection of the sample. Attached Figure Description

[0016] Figure 1 This is a structural diagram of the present utility model;

[0017] Figure 2 This is a schematic diagram of the signal light path and the indicator light path of this utility model. Detailed Implementation

[0018] The technical solution of this utility model will be further described below with reference to the accompanying drawings.

[0019] like Figure 1As shown, this embodiment of the invention provides a micro-area reflection interferometric spectroscopy detection system, comprising: a halogen lamp light source 1 for generating incident light; a first prism 2 and a second prism 3 for controlling the incident and reflected light paths; an objective lens 4 equipped with an aperture to control the focusing range for micro-area detection; a reflector 5 for reflecting the transmitted light from the second prism 3; an eyepiece 6 for receiving the light reflected by the reflector 5; a spectrometer 7 for acquiring and analyzing optical signals; a displacement stage 8 that moves along the X, Y, and Z directions for precise positioning and dynamic measurement; and a flow cell 9 for holding the interferometric film sample. A computer interface is also included for connecting the spectrometer 7 and a camera to achieve automatic data acquisition, storage, and analysis.

[0020] The second prism 3 splits the reflected light from the flow cell 9 into two parts: one part is reflected to the fiber optic input of the spectrometer 7, and the other part, through the lens, is reflected by the mirror 5 to the eyepiece 6 or a connected camera. The spectrometer 7 includes a CCD detector to receive the optical signal transmitted through the fiber optic cable and output the data to a computer for real-time processing and analysis. The aperture of the objective lens 4 is adjustable to focus light down to the micrometer scale, optimizing spatial resolution. The XYZ triaxial movement accuracy of the displacement stage 8 is at the micrometer level, which, combined with the controllable position of the flow cell 9, enables real-time dynamic measurement of biomolecular interactions. The flow cell 9 is detachable and is used to support an ordered porous nanostructured thin film as an interference sensing medium, supporting the detection of label-free biomolecules. The positions of the first prism 2, the second prism 3, and the mirror 5 are adjustable to optimize the incident and reflection angles.

[0021] like Figure 2 As shown, the optical path design is based on an inverted microscope architecture. The thick line represents the signal light path, and the thin line represents the indicator light path.

[0022] Signal light path: The light emitted by the halogen lamp 1 is reflected after encountering the first prism 2. The reflected light travels upward to the flow cell 9 on the stage 8 and illuminates the interference film. The reflected light (interference light) returning from the interference film is split into two beams by the second prism 3. One beam is transmitted, encounters the reflecting mirror 5, and exits through the eyepiece 6, which is observed by the experimenter or captured by a camera. The camera is connected to a computer and can display the image through calculation. The other part of the reflected beam is received by an optical fiber and input to a CCD. The CCD is connected to a computer, which outputs the received data to the computer for user processing and analysis.

[0023] Signal light path: The light source of the indicator light path comes from the spectrometer. The spectrometer light source is directly connected to the optical fiber output. The optical fiber projects the light onto the second prism 3, where it is reflected. The emitted light shines upward along the internal optical path of the device onto the sample to be sampled. The reflected light from the sample returns along the original path and is transmitted through the second prism 3 to the reflector 5, where it is reflected into the eyepiece or camera. The camera is connected to the computer to display the light image to the experimenter.

Claims

1. A micro-area reflectance interferometric spectroscopy detection system, characterized in that, include: A halogen lamp light source (1) is used to generate incident light; a first prism (2) and a second prism (3) are used to control the incident and reflected light paths; an objective lens (4) is equipped with an aperture to control the focusing range and realize micro-area detection; a reflector (5) is used to reflect the transmitted light from the second prism (3); an eyepiece (6) is used to receive the light reflected by the reflector (5); a spectrometer (7) is used to collect and analyze light signals; a displacement stage (8) moves along the XYZ directions for precise positioning and dynamic measurement; and a flow cell (9) is used to hold the interference film sample.

2. The micro-area reflection interferometric spectroscopy detection system according to claim 1, characterized in that, The system also includes a computer interface for connecting the spectrometer (7) and the camera to enable automatic data acquisition, storage and analysis.

3. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The system is divided into a signal light path and an indicator light path; the indicator light source of the indicator light path comes from the spectrometer (7) and is output to the second prism (3) through the optical fiber; the indicator light path is used to assist in signal acquisition.

4. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The second prism (3) is used to split the reflected light from the flow cell (9) into two parts: one part of the transmitted light is reflected by the mirror (5) to the eyepiece (6) or the connected camera, and the other part of the reflected light is sent to the fiber optic input of the spectrometer (7).

5. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The spectrometer (7) includes a CCD detector for receiving optical signals transmitted through optical fibers and outputting the data to a computer for real-time processing and analysis.

6. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The aperture of the objective lens (4) can be adjusted to focus light up to the micrometer scale, thereby optimizing the spatial resolution.

7. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The XYZ three-dimensional movement accuracy of the displacement stage (8) is at the micrometer level. Combined with the controllable position of the flow cell (9), it enables real-time dynamic measurement of biomolecular interactions.

8. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The optical path design is based on the inverted microscope architecture, including a halogen lamp light source (1) which is reflected to the flow cell (9) through the first prism (2), and the reflected light returning from the interference film is split by the second prism (3), with one beam entering the spectrometer (7) and the other entering the eyepiece (6).

9. The micro-area reflectance interferometric spectroscopy detection system according to claim 1, characterized in that, The flow cell (9) is configured to be detachable and is used to carry ordered porous nanostructured films as interference sensing media to support the detection of label-free biomolecules.

10. A micro-area reflection interferometric spectroscopy detection system according to claim 1, characterized in that, The positions of the first prism (2), the second prism (3), and the reflector (5) are adjustable to optimize the incident angle and the reflection angle.

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