Electric sliding rail type intelligent portable fluorescence detection device

Abstract

The utility model relates to an electric slide rail type intelligent portable fluorescence detection device belongs to medical detection equipment field, and this device contains power transmission module and fluorescence detection module, and power transmission module includes step motor, connecting rod, gear, arc slide rail, and fluorescence detection module includes light source, spectroscope, photoelectric detector. In power transmission module, step motor drives gear to move on arc slide rail through connecting rod, in fluorescence detection module, light source generates excitation wavelength, and the reflection of spectroscope is irradiated to the measured sample, and the emission wavelength of measured sample enters photoelectric detector through the refraction of spectroscope, and photoelectric detector converts fluorescence signal into electric signal. The utility model provides fluorescence detection device, has the outstanding advantage that the detection signal is stable, lightweight, intelligent, and can be used in biological sample detection, environmental monitoring, engineering quality control, food safety and other fields.

Description

Technical Field

[0001] This invention relates to the field of detection equipment technology, specifically to an electric sliding rail type intelligent portable fluorescence detection device. Background Technology

[0002] Fluorescence detection technology, due to its significant advantages such as high sensitivity, high selectivity, and non-destructive nature, is widely used in many fields, including life sciences (such as DNA sequencing, protein analysis, and cell imaging), medical diagnostics (such as pathogen detection and tumor marker analysis), environmental monitoring (such as pollutant detection), food safety (such as pesticide residue and toxin detection), and materials science. Traditional fluorescence detection mainly relies on large, complex laboratory equipment, such as fluorescence spectrophotometers, fluorescence microscopes, or fluorescence microplate readers. These devices are typically bulky, expensive, and require specialized operators and stable laboratory environments, greatly limiting the application of fluorescence detection in environments requiring rapid on-site screening, immediate diagnosis, or limited resources (such as primary healthcare institutions, field sites, and emergency response sites).

[0003] To meet the needs of on-site testing, some portable fluorescence detection devices have emerged on the market. However, most existing portable devices still have significant shortcomings in terms of performance, flexibility, and intelligence. First, in terms of detection range and flexibility, existing devices are limited by fixed or limited manually adjustable optical path designs, and their excitation light illumination and fluorescence signal reception angles are usually fixed and narrow. This makes it difficult for them to comprehensively capture fluorescence signals from irregular sample surfaces or different depths of large-volume samples. Especially for applications requiring wide-angle scanning to obtain representative data (such as heterogeneous solutions or tissue surface imaging), the representativeness and accuracy of their detection results are easily affected. Second, existing devices are usually single-function and have weak data processing capabilities. They can usually only display raw fluorescence signals or simple ratios and cannot perform complex algorithm analysis or automatic comparison with standard curves.

[0004] Therefore, the market urgently needs a solution that achieves a better balance between portability, high performance, and intelligence. An ideal portable fluorescence detection device not only needs to maintain a lightweight and compact design to ensure true portability and ease of on-site operation, but also needs to achieve breakthroughs in core detection capabilities: it should possess active, wide-area scanning capabilities to achieve efficient coverage and acquisition of fluorescence signals across wide angles and multiple regions, significantly improving the comprehensiveness of detection and its adaptability to complex samples; simultaneously, it needs to integrate a high-performance photoelectric signal conversion module, supplemented by optimized optical design, to ensure high-sensitivity, low-noise, and reliable electrical signal output even with a lightweight structure. Developing such intelligent fluorescence detection devices that integrate wide-angle automated scanning, high-performance photoelectric conversion, and true portability is of great significance for promoting accurate and efficient on-site fluorescence detection. Utility Model Content

[0005] (a) Technical problems to be solved

[0006] To address the aforementioned background, this utility model provides an electric sliding rail type intelligent portable fluorescence detection device, comprising a power transmission module and a fluorescence detection module. The power transmission module includes a stepper motor, connecting rod, gears, and an arc-shaped sliding rail; the fluorescence detection module includes a light source, a beam splitter, and a photodetector. This fluorescence detection device can achieve wide-angle, large-range, and automated fluorescence detection, solving the problems of limited detection range, insufficient detection flexibility, and difficult signal reading in existing fluorescence detection devices.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] In a first aspect, this utility model provides a design for a power transmission module of a fluorescence detection device: the power transmission module includes a stepper motor, a connecting rod, a gear, and an arc-shaped slide rail. In the power transmission module, the stepper motor drives the gear to move on the arc-shaped slide rail through the connecting rod; the arc-shaped slide rail is used to guide the movement of the detection module to achieve multi-angle and wide-range detection.

[0010] Preferably, the stepper motor has 2, 3, or 5 phases;

[0011] Preferably, the stepper motor has a step angle of 0.36-1.8°;

[0012] Preferably, the stepper motor has a torque greater than 0.5 Nm;

[0013] Preferably, the connecting rod is a cylinder;

[0014] Preferably, the diameter of the connecting rod is 2-20 mm;

[0015] Preferably, the module of the gear is 1-4;

[0016] Preferably, the number of teeth of the gear is 10-50;

[0017] Preferably, the module of the arc-shaped slide rail is 1-4;

[0018] Preferably, the arc-shaped slide rail has 100-500 teeth;

[0019] Preferably, the arc of the arc-shaped slide rail is 90-270°;

[0020] Preferably, the diameter of the arc-shaped slide rail is 5-50 cm.

[0021] In another aspect, this utility model provides the design of a detection module for a fluorescence detection device; the fluorescence detection module includes a light source, a beam splitter, and a photodetector; the light source generates excitation light, which is reflected by the beam splitter and irradiated onto the sample to be tested; the emission wavelength generated by the sample to be tested is refracted by the beam splitter and enters the photodetector; the photodetector converts the fluorescence signal into an electrical signal.

[0022] Preferably, the light source is an LED lamp, a halogen lamp, or a xenon lamp;

[0023] Preferably, the wavelength generated by the light source is 250-650 nm;

[0024] Preferably, the reflection wavelength range of the beam splitter is 250-650 nm;

[0025] Preferably, the refractive wavelength range of the beam splitter is 300-750 nm;

[0026] Preferably, the length of the beam splitter is 2-8 cm;

[0027] Preferably, the width of the beam splitter is 1-4 cm;

[0028] Preferably, the thickness of the beam splitter is 1-4 mm;

[0029] Preferably, the photodetector is a photodiode or a photomultiplier tube;

[0030] In another aspect, this utility model provides a method for preparing a fluorescence detection device, comprising the following steps:

[0031] S1: Draw engineering drawings of each component using 3D modeling software, including connecting rods, gears, slide rails, housings, etc., and determine the angles and distances between each component;

[0032] S2: Use machining methods to produce physical prototypes of each component;

[0033] S3: Assemble the components to obtain the fluorescence detection device.

[0034] Preferably, the arrangement angle between the light source and the photodetector is 90°;

[0035] Preferably, the angle between the beam splitter, the light source, and the photodetector is 45°, so that the excitation light and the emission light are at 90°, minimizing interference.

[0036] Preferably, the distance between the light source and the beam splitter is 4-12 cm;

[0037] Preferably, the distance between the beam splitter and the photodetector is 4-12 cm;

[0038] Preferably, the length of the outer shell is 8-24 cm;

[0039] Preferably, the width of the outer shell is 4-12 cm;

[0040] Preferably, the height of the outer shell is 4-12 cm;

[0041] The machining method is photocrosslinking 3D printing or thermal crosslinking;

[0042] Preferably, the processing material is photosensitive resin, polylactic acid, acrylonitrile butadiene styrene, or thermoplastic polyurethane;

[0043] Preferably, the thickness of the 3D printed slice layer is 0.035 mm;

[0044] Preferably, the exposure time for the photocrosslinking 3D printing is 2-10 s;

[0045] Preferably, the temperature for thermal crosslinking 3D printing is 190-220℃.

[0046] (III) Beneficial Effects

[0047] The beneficial effects of this invention are:

[0048] (1) The fluorescence detection device prepared by this utility model has a large detection angle and a wide detection range. The fluorescence detection device designed by this utility model includes a power transmission module. The power transmission module contains an innovatively designed arc-shaped slide rail structure. Through a high-precision stepper motor, the driving gear meshes and transmits on the slide rail, so that the fluorescence detection module moves along the slide rail and completes automatic scanning, realizing dynamic coordinated adjustment of the excitation light incident angle and the fluorescence receiving angle. This design enables the device to actively adapt to different sample morphologies: for side-flow chromatography test strips, vertical incident light can be used to avoid background interference; for deep-well plate samples, it can be switched to a large-angle oblique incident light to enhance bottom signal acquisition; for tissue surface imaging, a three-dimensional fluorescence distribution map is synthesized through multi-angle scanning. Combined with a broadband light source and replaceable filter wheels, the device is compatible with multiple fluorescent dyes from ultraviolet to near-infrared (such as FITC, Cy5, quantum dots), meeting the needs of complex scenarios such as multi-index joint detection of environmental pollutants and multiple nucleic acid detection of pathogens, greatly expanding the application boundaries of portable devices.

[0049] (2) The fluorescence detection device prepared by this invention is automated in operation and more intelligent in detection. The fluorescence detection device designed by this invention uses a high-precision stepper motor to drive the fluorescence detection module to move on the slide rail. The entire process is controlled by a single-chip microcomputer, which can complete specific movement angles and distances based on preset logic. At the same time, in the fluorescence detection module, the light source passes through a filter that matches the excitation wavelength, is reflected by a beam splitter, and reaches the object being tested. The emission wavelength generated by the object being tested is refracted by the beam splitter and reaches the photoelectric detection module, finally forming an electrical signal, and the fluorescence signal value is automatically read. The entire fluorescence detection process is automated without human operation or intervention, significantly reducing the operation threshold and human error.

[0050] (3) The fluorescence detection device prepared by this invention has accurate detection and digital display readings. In the fluorescence detection device designed by this invention, the beam splitter reflects the excitation wavelength and refracts the emission wavelength; the beam splitter is at a 45° angle to the light source and the photoelectric detection module, so that the excitation wave and the emission wave are separated by 90°, minimizing the detection interference of the excitation wave on the emission wave. The fluorescence detection device designed by this invention converts the original fluorescence signal into an electrical signal through a photoelectric converter, and finally displays the reading digitally.

[0051] (4) The fluorescence detection device prepared by this invention is lightweight and portable. The fluorescence detection device designed by this invention achieves a balance between performance and lightweight through innovation in materials science and structural engineering. The fluorescence detection device designed by this invention uses a small precision stepper motor to control the meshing transmission of gears on a slide rail, uses a beam splitter to separate the excitation wave and the emission wave, and uses a photoelectric detection module to convert the fluorescence signal into an electrical signal, thus achieving high-precision, lightweight, and portable fluorescence detection. It shows good application prospects in fields such as biological sample detection, environmental monitoring, engineering quality control, and food safety. Attached Figure Description

[0052] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly described below.

[0053] Figure 1 : A schematic diagram of the structure of this utility model, wherein: 1-power transmission module, 2-fluorescence detection module, 3-stepper motor, 4-connecting rod, 5-gear, 6-arc slide rail, 7-light source, 8-beam splitter, 9-photodetector, 10-shell.

[0054] Figure 2 3D modeling design of the power transmission module. A: 3D model of the gear; B: 3D model of the slide rail.

[0055] Figure 3Assembly of the fluorescence detection device (front view)

[0056] Figure 4 : Sensing and reading of photodetectors. Detailed Implementation

[0057] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0058] This invention designs an electric sliding rail type intelligent portable fluorescence detection device. Compared with other existing fluorescence detection devices, this device has significant advantages such as wide detection angle, large detection range, accurate detection signal, automated operation, and portability and lightweight design. Figure 1 As shown, the fluorescence detection device includes a power transmission module 1 and a fluorescence detection module 2; the power transmission module 1 includes a stepper motor 3, a connecting rod 4, a gear 5, and an arc-shaped slide rail 6; the fluorescence detection module 2 includes a light source 7, a beam splitter 8, and a photodetector 9; the stepper motor 3, the connecting rod 4, the light source 7, the beam splitter 8, and the photodetector 9 are all encapsulated in a housing.

[0059] The preparation method of this utility model of an electric sliding rail type intelligent portable fluorescence detection device includes the following steps:

[0060] (1) Design of the power transmission module: The power transmission module includes a stepper motor, connecting rod, gear, and arc-shaped slide rail. Among them, the stepper motor provides the power source for the movement of the detection module in the guide rail, and the power is transmitted through the connecting rod to drive the gear to move on the arc-shaped slide rail.

[0061] In some implementation examples, the stepper motor has 2, 3, or 5 phases;

[0062] In some implementation examples, the stepper motor has a step angle of 0.36-1.8°;

[0063] In some implementation examples, the torque of the stepper motor is greater than 0.5 Nm;

[0064] In some implementation examples, the connecting rod is a cylinder;

[0065] In some implementation examples, the diameter of the connecting rod is 2-20 mm;

[0066] In some implementation examples, the module of the gear is 1-4;

[0067] In some implementation examples, the number of teeth of the gear is 10-50;

[0068] In some implementation examples, the module of the arc-shaped slide rail is 1-4;

[0069] In some implementation examples, the number of teeth on the arc-shaped slide rail is 100-500;

[0070] In some implementation examples, the arc of the curved slide rail is 90-270°;

[0071] In some implementation examples, the diameter of the arc-shaped slide rail is 5-50 cm.

[0072] (2) Design of fluorescence detection module: The fluorescence detection module includes a light source, a beam splitter, and a photodetector; the light source generates excitation light, which is reflected by the beam splitter and irradiated onto the sample to be tested; the emission wavelength generated by the sample to be tested enters the photodetector through the refraction of the beam splitter; the photodetector converts the fluorescence signal into an electrical signal.

[0073] In some implementation examples, the light source is an LED lamp, or a halogen lamp, or a xenon lamp;

[0074] In some implementation examples, the wavelength produced by the light source is 250-650 nm;

[0075] In some implementation examples, the reflection wavelength range of the beam splitter is 250-650 nm;

[0076] In some implementation examples, the refractive wavelength range of the beam splitter is 300-750 nm;

[0077] In some implementation examples, the length of the beam splitter is 2-8 cm;

[0078] In some implementation examples, the width of the beam splitter is 1-4 cm;

[0079] In some implementation examples, the thickness of the beam splitter is 1-4 mm;

[0080] In some implementation examples, the photodetector is a photodiode or a photomultiplier tube;

[0081] (3) Physical fabrication and assembly of the fluorescence detection device: including the following steps:

[0082] S1: Based on the engineering drawings of each component drawn by 3D modeling software, including connecting rods, gears, slide rails, and housings, use machining methods to produce physical objects of each component;

[0083] S2: Assemble the components to obtain the fluorescence detection device.

[0084] In some implementation examples, the arrangement angle between the light source and the photodetector is 90°;

[0085] In some implementation examples, the angle between the beam splitter, the light source, and the photodetector is 45°, so that the excitation light and the emission light are at 90°, minimizing interference.

[0086] In some implementation examples, the distance between the light source and the beam splitter is 4-12 cm;

[0087] In some implementation examples, the distance between the beam splitter and the photodetector is 4-12 cm;

[0088] In some implementation examples, the length of the outer shell is 8-24 cm;

[0089] In some implementation examples, the width of the outer shell is 4-12 cm;

[0090] In some implementation examples, the height of the outer casing is 4-12 cm;

[0091] In some implementation examples, the machining method is photocrosslinking 3D printing or thermal crosslinking;

[0092] In some implementation examples, the processing material is photosensitive resin, polylactic acid, acrylonitrile butadiene styrene, or thermoplastic polyurethane;

[0093] In some implementation examples, the thickness of the 3D printed slice layer is 0.035 mm;

[0094] In some implementation examples, the exposure time for the photocrosslinking 3D printing is 2-10 s;

[0095] In some implementation examples, the temperature of the thermal crosslinking 3D printing is 190-220°C.

[0096] The following are specific embodiments.

[0097] Example 1: Design of the power transmission module

[0098] like Figure 2 As shown, the power transmission module is designed and modeled in 3D software. The gear module is selected as 1, the number of teeth as 10, and the gear type as a spur gear, resulting in a 3D model of the gear, as shown below. Figure 2 A.

[0099] The gear is designed and modeled in 3D software. The gear module is selected as 1, the number of teeth as 10, and the gear type as a spur gear. Using the top reference plane and the origin as the center, a primitive element with a diameter of 10*cos(pi / 9) mm is drawn. The spline is driven by an equation:

[0100] xt=1*10*cos(pi / 9) / 2*sin(t)-1*10*cos(pi / 9) / 2*t*cos(t)

[0101] yt=1*10*cos(pi / 9) / 2*cos(t)+1*10*cos(pi / 9) / 2*t*sin(t)

[0102] Obtain the spline. Using the origin as the center, draw a 12 mm diameter addendum circle; draw a 7.5 mm diameter addendum circle; draw a 10 mm diameter pitch circle. Draw a spline from the intersection of the base circle and the involute to the dedendum circle, ensuring the spline is tangent to the involute. Draw a centerline through the center and the intersection of the pitch circle and the involute. Draw symmetry axes 1 and 2 through the center; the angle between symmetry axis 1 and the centerline is 9°, and the angle between symmetry axis 2 and the centerline is 27°. Mirror the involute and spline with symmetry axis 1 as the first axis of symmetry, and then mirror them a second time with symmetry axis 2 as the second axis of symmetry. Array the above contours circumferentially along the addendum circle, with an array size of 10. Based on the above sketch, extrude the boss to a thickness of 5 mm to obtain the 3D design model of the gear.

[0103] The curved slide rail is designed and modeled in 3D software. The module of the curved slide rail is selected as 1, the number of teeth as 200, and the gear type as a spur gear. Taking the top reference plane, with the origin as the center, a primitive element with a diameter of 200*cos(pi / 9) mm is drawn. The spline is driven by an equation, using the following equation:

[0104] xt=1*200*cos(pi / 9) / 2*sin(t)-1*200*cos(pi / 9) / 2*t*cos(t)

[0105] yt=1*200*cos(pi / 9) / 2*cos(t)+1*200*cos(pi / 9) / 2*t*sin(t)

[0106] Obtain the spline. Using the origin as the center, draw a tooth tip circle with a diameter of 202 mm; draw a tooth tip circle with a diameter of 197.5 mm; draw a pitch circle with a diameter of 200 mm. Draw a centerline through the center and the intersection of the pitch circle and the involute. Draw symmetry axis 1 and symmetry axis 2 through the center; the angle between symmetry axis 1 and the centerline is 0.45°, and the angle between symmetry axis 2 and the centerline is 1.35°. Mirror the involute firstly with symmetry axis 1 as the symmetry axis, and then mirror it a second time with symmetry axis 2 as the symmetry axis. Array the above contours along the tooth tip circle in a circle with a number of arrays of 200. Extrude the boss based on the above sketch, with an extrusion thickness of 5 mm. Using the origin as the center, draw a circle with a diameter of 190 mm and extrude it. Using the origin as the center, draw an arc with a radius of 101 mm and an arc degree of 180 degrees and extrude it, finally obtaining the 3D model of the curved slide rail, see [link to model]. Figure 2 B.

[0107] Example 2: Design of Fluorescence Detection Module

[0108] The fluorescence detection module comprises a light source, a beam splitter, and a photodetector. The light source generates excitation light, which is reflected by the beam splitter and irradiates the sample under test. The emission wavelength emitted by the sample is refracted by the beam splitter and enters the photodetector. The photodetector converts the fluorescence signal into an electrical signal. The selected light source is an LED lamp, producing an emission wavelength range of 485±10 nm. The selected beam splitter has a reflection wavelength of 485±10 nm and a refraction wavelength of 485±10 nm. The selected beam splitter is 2 cm long, 2 cm wide, and 2 mm thick.

[0109] Example 3: Preparation and assembly of a fluorescence detection device

[0110] Based on the 3D model, polylactic acid (PLA) was used as the printing resin, and the image was printed for 20 seconds using a photocrosslinking 3D printer; subsequent layers were exposed for 5 seconds each, resulting in physical models of the gears and curved guide rails. Figure 3 As shown, assemble the above components. The angle between the light source and the photodetector is 90°, and the angle between the beam splitter and both the light source and the photodetector is 45°. The distance between the light source and the beam splitter is 9 cm; the distance between the beam splitter and the photodetector is 5 cm.

[0111] Example 4: Sensing and Reading of a Photodetector

[0112] Photodetectors are made of semiconductor materials such as cadmium sulfide and cadmium selenide, and possess a unique photoelectric effect: light excites valence band electrons to transition to the conduction band, generating additional electron-hole pairs as charge carriers, thus significantly increasing the material's conductivity. Its resistance is inversely proportional to the incident light intensity. By connecting it to a voltage divider circuit, changes in light intensity can be converted into significant voltage changes, thereby achieving light intensity detection. Figure 4 As shown, as the light intensity increases, the detector resistance decreases, and the analog voltage reading from the detection circuit gradually increases, which is directly proportional to the light intensity.

[0113] In summary, this invention provides an electrically driven, sliding-rail type intelligent portable fluorescence detection device, comprising a power transmission module and a fluorescence detection module. The power transmission module includes a stepper motor, connecting rods, gears, and an arc-shaped slide rail; the fluorescence detection module includes a light source, a beam splitter, and a photodetector. This fluorescence detection device can achieve wide-angle, large-range, and automated fluorescence detection, solving the problems of limited detection range, insufficient detection flexibility, and difficult signal reading in existing fluorescence detection devices.

[0114] Finally, it should be noted that the above embodiments are for illustrative purposes only and are not intended to limit the scope of protection of the present invention. Furthermore, after reading the technical content of this invention, those skilled in the art can make various modifications, alterations, or variations to the invention, and all such equivalent forms also fall within the scope of protection claimed in this application.

Claims

1. An electrically powered sliding rail based intelligent portable fluorescence detection device, characterized in that, The system includes a power transmission module (1) and a fluorescence detection module (2). The power transmission module (1) includes a stepper motor (3), a connecting rod (4), a gear (5), and an arc-shaped slide rail (6). The stepper motor (3) drives the gear (5) through the connecting rod (4). The gear (5) meshes with the arc-shaped slide rail (6). The fluorescence detection module (2) includes a light source (7), a beam splitter (8), and a photodetector (9). The arrangement angle between the light source (7) and the photodetector (9) is 90°. The arrangement angle between the beam splitter (8) and the light source (7) and the photodetector (9) is 45°. The stepper motor (3), the connecting rod (4), the light source (7), the beam splitter (8), and the photodetector (9) are all encapsulated in a housing (10).

2. The electrically powered smart portable fluorescent detection device of claim 1, wherein, The torque of the stepper motor (3) is greater than 0.5 Nm.

3. The electrically powered smart portable fluorescent detection device of claim 1, wherein, The gear (5) has a module of 1-4 and a number of teeth of 10-50.

4. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The arc-shaped slide rail (6) has a module of 1-4 and a number of teeth of 100-500.

5. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The arc of the arc-shaped slide rail (6) is 90-270°.

6. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The reflection wavelength range of the beam splitter (8) is 250-650 nm, and the refraction wavelength range of the beam splitter (8) is 300-750 nm.

7. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The beam splitter (8) has a length of 2-8 cm, a width of 1-4 cm, and a thickness of 1-4 mm.

8. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The distance between the light source (7) and the beam splitter (8) is 4-12 cm, and the distance between the beam splitter (8) and the photodetector (9) is 4-12 cm.

9. The electric sliding rail type intelligent portable fluorescence detection device as described in claim 1, characterized in that, The outer shell (10) is 8-24 cm long, 4-12 cm wide, and 4-12 cm high.

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