Droplet microfluidic cell sorter

Abstract

The utility model relates to cell sorting technical field discloses a kind of droplet microfluidic cell sorters, comprising: detection component, including imaging piece and light source piece, imaging area is formed between the imaging piece and the light source piece;Bracket, for carrying microfluidic chip, the bracket is set in the imaging area;Alignment component, set in the bracket below, the alignment component adjusts the position of the microfluidic chip in the imaging area;And pumping component, for even pumping cell suspension to the microfluidic chip place.The utility model plays the technical effect of improving the precision of target cell sorting.

Description

Technical Field

[0001] This invention belongs to the field of cell sorting technology, specifically relating to a droplet microfluidic cell sorting instrument. Background Technology

[0002] Droplet microfluidics is a technology that uses "microscale droplets" as independent operating units to precisely control the behavior of fluids within microchannels, enabling the generation, manipulation, detection, and sorting of droplets. The microscale efficiency, high throughput, monodispersity, closed-loop nature, and independence of droplet microfluidics have led to its widespread application in the biomedical field, particularly in the precise sorting of single cells, such as in the capture of circulating tumor cells, stem cell screening, preparation of single-cell sequencing samples, and drug sensitivity testing.

[0003] A droplet microfluidic cell sorter is a cell separation device based on droplet microfluidic technology, typically comprising a droplet generation unit, a detection unit, and a sorting unit. The droplet generation unit includes a microfluidic chip. During droplet formation, an aqueous phase containing cells is mixed with an immiscible oil phase within the microchannels of the microfluidic chip, generating a water-in-oil droplet. The detection unit includes an imaging component for observing the microfluidic chip. This component identifies and records the dynamic behavior and characteristics of cells, screening for target cells by recognizing specific signals within the droplets. Droplets containing target cells are then separated from the main channel by the sorting unit, thus achieving cell sorting.

[0004] However, during the observation and identification of cells, the imaging components may experience optical path alignment deviations, affecting the sorting accuracy of target cells. Utility Model Content

[0005] To address the shortcomings of the prior art, this invention provides a droplet microfluidic cell sorting instrument that ensures the optical path alignment between the detection component and the microfluidic chip through an alignment component, thereby improving the accuracy of target cell sorting.

[0006] The technical effects to be achieved by this utility model are realized through the following technical aspects: This invention provides a droplet microfluidic cell sorting device, comprising: a detection component including an imaging element and a light source, wherein an imaging area is formed between the imaging element and the light source; a bracket for supporting a microfluidic chip, wherein the bracket is disposed within the imaging area; an alignment component disposed under the bracket, wherein the alignment component adjusts the position of the microfluidic chip within the imaging area; and a pumping component disposed on one side of the detection component for pumping a cell suspension to the microfluidic chip.

[0007] In some implementations, the alignment component includes: a stage on which the bracket is disposed; a first adjustment structure including a first base plate and a first driving member, the first base plate being slidably connected to the stage and the first driving member being drively connected to the stage to drive the stage to slide relative to the first base plate along the Y-axis; a second adjustment structure including a second base plate and a second driving member, the second base plate being slidably connected to the first base plate and the second driving member being drively connected to the first base plate to drive the first base plate to slide relative to the second base plate along the X-axis; and a third adjustment structure being drively connected to the second base plate, the third adjustment structure driving the second base plate to move and adjust along the Z-axis.

[0008] In some implementations, a first guide rail is provided on the first base plate, and the stage is slidably connected to the first guide rail to move relative to the first base plate; a second guide rail is provided on the second base plate, and the first base plate is slidably connected to the second guide rail to move relative to the second base plate.

[0009] In some implementations, a first inductive sensor for positioning the stage is provided at one end of the first guide rail in the Y-axis direction, and the first inductive sensor is electrically connected to the first driving component; a second inductive sensor for positioning the first base plate is provided at one end of the second guide rail in the X-axis direction, and the second inductive sensor is electrically connected to the second driving component.

[0010] In some implementations, the first guide rail and / or the second guide rail are crossed roller guide rails.

[0011] In some implementations, the first adjustment structure includes: a first connecting block connected to the stage; and a first lead screw passing through the first connecting block and threadedly connected to the first connecting block, the first lead screw being throttle-connected to the first driving member, the first driving member driving the first lead screw to rotate; the first driving member includes a first rotary transformer.

[0012] In some implementations, a liquid storage component for conveying the liquid phase and the oil phase is provided on one side of the alignment component; The liquid storage assembly includes a liquid storage tube for storing a liquid phase or an oil phase. Multiple liquid storage tubes are provided. Each liquid storage tube has an output hole and a vent hole. The liquid storage tube delivers the liquid phase or oil phase through the output hole. A pneumatic connector is provided at the vent hole of the liquid storage tube, and the pneumatic connector is connected to the liquid storage tube.

[0013] In some implementations, the pumping assembly includes: a container for holding a cell suspension, the container having an outlet for discharging the cell suspension; and a mixing element, drivenly connected to the container, the mixing element shaking the container.

[0014] In some implementations, the bracket has an observation hole, the imaging element is located at the bottom of the bracket, and the microfluidic chip is imaged through the observation hole. The light source is located above the bracket.

[0015] In some implementations, a high-pressure component for sorting cells at the microfluidic chip is provided on one side of the alignment component.

[0016] In summary, this utility model has at least the following advantages: The droplet microfluidic cell sorting instrument provided by this invention features a microfluidic chip fixedly placed on a holder before cell sorting, ensuring the chip is within the imaging area. The holder is adjusted by a positioning component, aligning the microfluidic chip to a suitable position within the imaging area, ensuring the imaging element, light source, and microfluidic chip are aligned on the same optical path. During cell sorting, a pumping component uniformly pumps cell suspension to the microfluidic chip, the light source provides illumination, the imaging element accurately captures images of the microfluidic chip, and the detection component accurately identifies and records cell dynamics and characteristics, thus achieving cell sorting and improving the accuracy of target cell sorting. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a droplet microfluidic cell sorter according to a specific embodiment of the present invention.

[0018] Figure 2 This is a schematic diagram of the detection component and pumping component in a specific embodiment of the present invention.

[0019] Figure 3 This is an exploded view of the alignment component according to a specific embodiment of the present invention.

[0020] Figure 4 This is a schematic diagram of the liquid storage component according to a specific embodiment of the present invention.

[0021] Figure 5 This is a schematic diagram of the bracket structure in a specific embodiment of the present utility model.

[0022] Figure 6 This is a schematic diagram of the high-voltage component according to a specific embodiment of the present invention.

[0023] Marked in the image: 1. Detection component; 11. Imaging component; 111. Inverted microscope; 12. Light source component; 13. Imaging area; 2. Bracket; 21. Observation hole; 3. Alignment assembly; 31. Stage; 32. First adjustment structure; 321. First base plate; 322. First driving component; 323. First guide rail; 324. First fixed base; 325. First connecting block; 326. First lead screw; 327. First inductive sensor; 33. Second adjustment structure; 331. Second base plate; 332. Second driving component; 333. Second guide rail; 334. Second fixed base; 335. Second connecting block; 336. Second lead screw; 337. Second inductive sensor; 34. Third adjustment structure; 341. Base; 342. Third driving component; 4. Pumping assembly; 41. Container; 42. Mixing component; 5. Liquid storage assembly; 51. Liquid storage pipe; 511. Output port; 512. Vent port; 513. Pneumatic connector; 6. High-voltage components; 61. PCB power control module; 7. Microfluidic chip; 8. Rack. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. The described embodiments are some, but not all, of the embodiments of this utility model.

[0025] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0026] Example 1: Please see Figure 1 The droplet microfluidic cell sorting instrument of this invention can achieve single-cell sorting through droplet microfluidic technology and improve the accuracy of cell sorting.

[0027] The present invention relates to a droplet microfluidic cell sorting instrument, which includes a detection component 1 for observing the dynamics of droplets and cells in a microfluidic chip 7. The detection component 1 includes an imaging element 11 and a light source element 12. An imaging area 13 is formed between the imaging element 11 and the light source element 12. The light source element 12 provides a light source. The imaging element 11 accurately captures images of the microfluidic chip 7 in the imaging area 13 to obtain cell images.

[0028] The detection component 1 is provided with a bracket 2 for carrying the microfluidic chip 7 in the imaging area 13. The bracket 2 is located in the imaging area 13. In some specific embodiments, the bracket 2 is provided with elastic clips. The microfluidic chip 7 can be clamped and fixed on the bracket 2 by the elastic clips to ensure the stability of the microfluidic chip 7 during cell sorting operation.

[0029] An alignment component 3 is provided under the bracket 2. The alignment component 3 adjusts the position of the microfluidic chip 7 in the imaging area 13 through the bracket 2, so that the imaging element 11, the light source 12 and the microfluidic chip 7 are in the same optical path. The imaging element 11 clearly captures the droplets and cells at the microfluidic chip 7, which is beneficial to improving the accuracy of cell sorting.

[0030] A pumping component 4 is provided on one side of the detection component 1. The pumping component 4 uniformly pumps the cell suspension to the microfluidic chip 7, where the cell suspension forms droplets and is sorted.

[0031] The microfluidic chip 7 is placed on the bracket 2 and fixed in the imaging area 13 by elastic clips. The alignment component 3 adjusts the position of the bracket 2 to keep the microfluidic chip 7 aligned with the imaging element 11 and the light source 12 in the imaging area 13, achieving optical path alignment. This helps to reduce the impact of optical path alignment deviation on the target cell sorting accuracy. After the microfluidic chip 7 is aligned with the imaging element 11 and the light source 12 by the alignment component 3, the pumping component 4 uniformly pumps the cell suspension into the microfluidic chip 7. The cell suspension disperses into single cells in the microfluidic chip, and the target cells are observed and identified by the imaging component. The target cells are then sorted at the microfluidic chip 7.

[0032] Example 2: The difference between this embodiment and Embodiment 1 is that this embodiment further optimizes the structure of the alignment component 3 of this utility model. Please refer to [link / reference]. Figure 2 and Figure 3 .

[0033] The alignment component 3 in this embodiment includes a stage 31, a first adjustment structure 32, a second adjustment structure 33, and a third adjustment structure 34. Specifically, the microfluidic chip 7 can be flexibly adjusted along the Y-axis, X-axis, and Z-axis respectively through the alignment component 3, and the three-axis adjustment is beneficial to meet the requirements of high-precision positioning.

[0034] The bracket 2 is mounted on the platform 31. Specifically, the bracket 2 and the platform 31 are detachably connected, which is beneficial for the maintenance of the alignment component 3.

[0035] The first adjustment structure 32 is disposed on the stage 31 and includes a first base plate 321 and a first driving member 322. The first base plate 321 is disposed at the bottom of the stage 31. The stage 31 can slide and adjust relative to the first base plate 321 along the Y-axis direction. The first driving member 322 is connected to the stage 31 in a transmission manner to drive the stage 31 to slide and adjust relative to the first base plate 321 along the Y-axis direction.

[0036] In some specific embodiments, the first adjustment structure 32 further includes a first fixed seat 324. The first fixed seat 324 is disposed on one side of the first base plate 321 along the Y-axis direction and is detachably connected to the first base plate 321. A first connecting block 325 is slidably disposed on the first fixed seat 324. The first connecting block 325 is connected to the platform 31. When the first connecting block 325 slides relative to the first fixed seat 324, it can drive the platform 31 to slide relative to the first base plate 321.

[0037] A first lead screw 326 is rotatably mounted on the first fixed base 324. The first lead screw 326 passes through the first connecting block 325 and is threadedly connected to the first connecting block 325. Specifically, the first lead screw 326 is preferably, but not limited to, a ball screw, which can ensure the accuracy and stability of adjustment and achieve precise fine-tuning of the stage 31. A first driving member 322 is connected to the first lead screw 326. The first driving member 322 includes a first motor and a first rotary transformer. The first motor is electrically connected to the first rotary transformer, and the output end of the first motor is connected to the first lead screw 326 to drive the first lead screw 326 to rotate. The first connecting block 325 moves along the first lead screw 326 and drives the stage 31 to adjust its position relative to the first base plate 321 along the Y-axis.

[0038] Furthermore, a mounting groove is provided on the first base plate 321, and a first guide rail 323 for guiding the sliding of the platform 31 is provided in the mounting groove. Multiple first guide rails 323 can be provided, and multiple first guide rails 323 are arranged on both sides of the first base plate 321 along the Y-axis direction. The first adjustment structure 32 has a compact layout, which can reduce the volume occupied.

[0039] The second adjustment structure 33 is disposed on one side of the first base plate 321. The second adjustment structure 33 includes a second base plate 331 and a second driving member 332. Specifically, the first base plate 321 is slidably disposed on the second base plate 331, and the first base plate 321 can slide and adjust relative to the second base plate 331 along the X-axis direction. The second driving member 332 is drively connected to the first base plate 321 to drive the first base plate 321 to slide relative to the second base plate 331 along the X-axis direction.

[0040] In some specific embodiments, the second adjustment structure 33 further includes a second fixed seat 334. The second fixed seat 334 is disposed on one side of the second base plate 331 along the X-axis direction and is detachably connected to the second base plate 331. A second connecting block 335 is slidably disposed on the second fixed seat 334. The second connecting block 335 is connected to the first base plate 321. When the second connecting block 335 slides relative to the second fixed seat 334, it can drive the first base plate 321 to slide relative to the second base plate 331.

[0041] A second lead screw 336 is rotatably mounted on the second fixed base 334. The second lead screw 336 passes through the second connecting block 335 and is threadedly connected to the second connecting block 335. Specifically, the second lead screw 336 is preferably, but not limited to, a ball screw, which can ensure the accuracy and stability of adjustment and achieve precise fine-tuning of the first base plate 321 in the X-axis direction. The second driving member 332 is connected to the second lead screw 336. The second driving member 332 includes a second motor and a second rotary transformer. The second motor is electrically connected to the second rotary transformer, and the output end of the second motor is connected to the second lead screw 336 to drive the second lead screw 336 to rotate. The second connecting block 335 moves along the second lead screw 336 and drives the first base plate 321 to adjust its position relative to the second base plate 331 in the X-axis direction.

[0042] Furthermore, the first base plate 321 is provided with a second guide rail 333 for guiding the sliding of the first base plate 321. Multiple second guide rails 333 can be provided, and multiple second guide rails 333 are provided on both sides of the second base plate 331 along the X-axis direction. Specifically, the first guide rail 323 and / or the second guide rail 333 are cross roller guide rails. Cross roller guide rails have line contact, small deformation and low coefficient of friction when subjected to force, which is beneficial to improving the stability and positioning accuracy of the movement of the stage 31 and the first base plate 321, and can meet the high-precision adjustment requirements of the microfluidic chip 7. The cross roller guide rail has a compact structure, which is beneficial to reducing the space occupied by the alignment component.

[0043] In some specific embodiments, a second inductive sensor 337 for positioning the first base plate 321 is provided at one end of the second guide rail 333 in the length direction. The second inductive sensor 337 is the limiting origin for the linear movement of the first base plate 321. The second inductive sensor 337 is electrically connected to the second rotary transformer. By converting the position signal into an electrical signal, the second rotary transformer can determine when the second motor switches the current direction and intensity, thereby controlling the rotation speed and torque, achieving high-precision positioning and reset after movement.

[0044] Similarly, a first inductive sensor 327 for positioning the stage 31 is provided at one end of the first guide rail 323 in the length direction. The first inductive sensor 327 is the limit origin for the linear movement of the stage 31 and is electrically connected to the first rotary transformer.

[0045] A base 341 is provided at the bottom of the second base plate 331, and a third adjustment structure 34 is disposed on the base 341 and located between the second base plate 331 and the base 341. The third adjustment structure 34 is drive-connected to the second base plate 331. In some specific embodiments, the third adjustment structure 34 includes a push block, which includes an inclined surface. A top block is provided on the inclined surface of the push block. The top block is connected to the second base plate 331, and a guide rail is provided between the top block and the base 341 to guide the top block to move along the Z-axis. The push block engages with the top block on the inclined surface, and a third drive member 342 is drive-connected to the push block to move horizontally along the X-axis. The third drive member 342 is preferably, but not limited to, a stepper motor and a ball screw.

[0046] The third driving component 342 drives the push block to move along the X-axis direction, and the push block pushes the top block to move along the Z-axis direction. At this time, the top block can drive the second base plate 331 to move synchronously along the Z-axis direction. The third adjustment structure 34 realizes the position adjustment of the second base plate 331 along the Z-axis direction.

[0047] In some specific embodiments, the bracket 2, the stage 31, the Y-axis base plate and the X-axis base plate are all provided with round holes, through which the alignment between the bracket 2, the stage 31, the Y-axis base plate and the X-axis base plate can be observed.

[0048] The microfluidic chip 7 achieves three-axis position adjustment through the first adjustment structure 32, the second adjustment structure 33 and the third adjustment structure 34, which can precisely control the movement position.

[0049] Example 3: The difference between this embodiment and the above embodiments is that, please refer to [link / reference needed]. Figure 4The alignment component 3 of this invention has a liquid storage component 5 for transporting liquid and oil phases on one side. In some specific embodiments, the liquid storage component 5 includes a liquid storage tube 51 for storing the liquid or oil phase, and multiple liquid storage tubes 51 are provided. Specifically, the liquid storage tube 51 includes a tube body and a sealing cap, which are threaded together and sealed by a sealing ring. The liquid storage tube 51 has an output hole 511 and a vent hole 512 on the sealing cap. The liquid storage tube 51 transports the liquid or oil phase through the output hole 511. Specifically, the liquid storage tube 51 has a delivery tube at the output hole 511, through which the liquid or oil phase is transported to the channel of the microfluidic chip 7. The sealing cap has a pneumatic connector 513 at the vent hole 512, which is connected to the liquid storage tube 51. The pneumatic connector 513 introduces gas into the liquid storage tube 51 to realize the pumping of the liquid or oil phase.

[0050] Please see Figure 2 In a preferred embodiment, the pumping assembly 4 includes a container 41 for holding the cell suspension. The container 41 has an outlet hole for discharging the cell suspension. The cell suspension is output from the outlet hole and flows to the microfluidic chip 7, where it forms water-in-oil droplets. Specifically, the container 41 can be externally connected to an air compression device such as a micro peristaltic pump and an EPC proportional valve to pump the cell suspension from the outlet hole into the microfluidic chip 7.

[0051] The container 41 is connected to a mixing element 42, which is preferably, but not limited to, a motor. The mixing element 42 shakes the container 41 to disperse the agglomerated or precipitated cells and deliver the cells evenly to the microfluidic chip 7, which is beneficial for cell sorting.

[0052] Please see Figure 5 In some specific embodiments, the bracket 2 has an observation hole 21, the microfluidic chip 7 is placed in the observation hole 21, the imaging element 11 is located at the bottom of the bracket 2, and the light source 12 is located at the top of the bracket 2. Specifically, the imaging element 11 includes an inverted microscope 111. The inverted microscope 111 reduces interference with the light source illumination. Because cells tend to sink, the inverted microscope 111 is beneficial for observing cultured live cells and tissues. The inverted microscope 111 is equipped with a phase contrast objective lens, which can clearly distinguish samples with high transparency and indistinct structural contrast, and allows the experimenter to observe the sample from below, providing more operating space for experimental operations. The inverted microscope 111 is equipped with a microscope camera, which is used to image the microdroplet chip.

[0053] Please see Figure 6In some specific embodiments, a high-voltage component 6 for sorting cells at the microfluidic chip 7 is disposed at the bottom of the alignment component 3. The high-voltage component 6 applies a high-voltage electric field, causing droplets to deflect rapidly within the microchannels of the microfluidic chip 7 to achieve cell sorting. The method of sorting cells using a high-voltage electric field is known to those skilled in the art and is achievable, and will not be described in detail in this embodiment. Furthermore, a PCB power control module 61 is disposed on one side of the alignment component 3. The PCB power control module 61 is electrically connected to the high-voltage component 6 and can control the high-voltage component 6 to perform cell sorting.

[0054] In some other specific embodiments, the droplet microfluidic cell sorter of this utility model also includes a frame 8, and the detection component 1, alignment component 3, pumping component 4, liquid storage component 5 and pumping component 4 are all disposed on the frame 8. The frame 8 is provided with a flip cover, which is hinged to the frame 8 to play a protective role.

[0055] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0056] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first," "second," and "third," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0057] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0058] In this invention, unless otherwise expressly specified and limited, "above or below" the first feature may include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on" the first feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the first feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0059] Although the description of this utility model has been given in conjunction with the specific embodiments described above, it is obvious to those skilled in the art that many substitutions, modifications, and variations can be made based on the above description. Therefore, all such substitutions, modifications, and variations are included within the spirit and scope of the appended claims.

Claims

1. A droplet microfluidic cell sorting instrument, characterized in that, include: The detection component (1) includes an imaging element (11) and a light source element (12), wherein an imaging area (13) is formed between the imaging element (11) and the light source element (12); A bracket (2) is used to support the microfluidic chip (7), and the bracket (2) is disposed within the imaging area (13); Alignment component (3) is disposed under the bracket (2), and the alignment component (3) adjusts the position of the microfluidic chip (7) in the imaging area (13); as well as The pumping component (4) is used to uniformly pump the cell suspension to the microfluidic chip (7).

2. The droplet microfluidic cell sorting instrument according to claim 1, characterized in that, The alignment component (3) includes: A platform (31) is provided on which the bracket (2) is mounted; The first adjustment structure (32) includes a first base plate (321) and a first driving member (322). The first base plate (321) is slidably connected to the stage (31), and the first driving member (322) is drively connected to the stage (31) to drive the stage (31) to slide relative to the first base plate (321) along the Y-axis direction. The second adjustment structure (33) includes a second base plate (331) and a second driving member (332). The second base plate (331) is slidably connected to the first base plate (321), and the second driving member (332) is driveably connected to the first base plate (321) to drive the first base plate (321) to slide relative to the second base plate (331) along the X-axis direction; and The third adjustment structure (34) is connected to the second base plate (331) in a transmission manner, and the third adjustment structure (34) drives the second base plate (331) to move and adjust along the Z-axis direction.

3. The droplet microfluidic cell sorting instrument according to claim 2, characterized in that, The first base plate (321) is provided with a first guide rail (323), and the platform (31) is slidably connected to the first guide rail (323) so as to move relative to the first base plate (321); The second base plate (331) is provided with a second guide rail (333), and the first base plate (321) is slidably connected to the second guide rail (333) so as to move relative to the second base plate (331).

4. The droplet microfluidic cell sorting device according to claim 3, characterized in that, The first guide rail (323) is provided with a first inductive sensor (327) at one end in the Y-axis direction for positioning the stage (31), and the first inductive sensor (327) is electrically connected to the first drive member (322); The second guide rail (333) is provided with a second inductive sensor (337) at one end in the X-axis direction for positioning the position of the first base plate (321), and the second inductive sensor (337) is electrically connected to the second drive member (332).

5. The droplet microfluidic cell sorting device according to claim 3 or 4, characterized in that, The first guide rail (323) and / or the second guide rail (333) are cross roller guide rails.

6. The droplet microfluidic cell sorting device according to claim 3, characterized in that, The first adjustment structure (32) includes: The first connecting block (325) is connected to the stage (31); and The first lead screw (326) is threaded through the first connecting block (325) and threadedly connected to the first connecting block (325). The first lead screw (326) is connected to the first driving member (322) in a transmission manner. The first driving member (322) drives the first lead screw (326) to rotate. The first drive unit (322) includes a first rotary transformer.

7. The droplet microfluidic cell sorting instrument according to claim 1, characterized in that, A liquid storage component (5) for conveying liquid and oil phases is provided on one side of the alignment component (3); The liquid storage assembly (5) includes a liquid storage pipe (51) for storing liquid or oil phase. Multiple liquid storage pipes (51) are provided. Each liquid storage pipe (51) has an output hole (511) and a vent hole (512). The liquid storage pipe (51) delivers liquid or oil phase through the output hole (511). A pneumatic connector (513) is provided at the vent hole (512) of the liquid storage pipe (51), and the pneumatic connector (513) is connected to the liquid storage pipe (51).

8. The droplet microfluidic cell sorting device according to claim 1, characterized in that, The pumping assembly (4) includes: A container (41) for holding a cell suspension, wherein the container (41) has an outlet hole for discharging the cell suspension; and A mixing component (42) is connected to the container (41) in a transmission manner, and the mixing component (42) shakes the container (41).

9. The droplet microfluidic cell sorting device according to claim 1, characterized in that, The bracket (2) is provided with an observation hole (21), the imaging element (11) is located at the bottom of the bracket (2), and the microfluidic chip (7) is photographed through the observation hole (21). The light source element (12) is located above the bracket (2).

10. The droplet microfluidic cell sorting device according to claim 1, characterized in that, A high-pressure component (6) for sorting cells at the microfluidic chip (7) is provided on one side of the alignment component (3).

Images