Multiple fluorescence immunoassay test paper for mycotoxin in agricultural products

Abstract

The utility model discloses a multiple fluorescence immunoassay test paper of mycotoxin in agricultural product, including the outer sheet, the inside array of outer sheet is provided with test paper, the right -hand side of test paper central place all is equipped with the flow guide groove, the right -hand side of flow guide groove is connected with water absorption pad each other, test paper and outer sheet are fixed each other between, the inside parallel of test paper in outer sheet, the surface of outer sheet is equipped with the observation groove, the surface of outer plate is penetrated by the observation groove, be provided with transparent film between observation groove and test paper, the right -hand side fixed setting of outer sheet is equipped with the tail plate, the position of the left side of tail plate is equipped with the buckle close to outer sheet, through being provided with multiple test paper, can detect multiple detection project in the device inside single time with same sample, when sample passes through water absorption pad and contacts test paper, sample will flow through flow guide groove and guide the left side of test paper, not only can make sample flow along the preset direction, avoid cross -contamination, can also optimize the utilization of sample.

Description

Technical Field

[0001] This utility model belongs to the field of rubber additives and technology, specifically relating to a multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products. Background Technology

[0002] Mycotoxin contamination in agricultural products is one of the major challenges to food safety. Mycotoxins are toxic secondary metabolites produced by toxin-producing fungi such as Aspergillus, Penicillium, and Fusarium during crop growth, harvesting, storage, or processing. They mainly include aflatoxin, zearalenone, deoxynivalenol, and ochratoxin A. These toxins are highly toxic, carcinogenic, teratogenic, and immunosuppressive. Even extremely low concentrations can cause serious harm to human health, such as liver damage, kidney dysfunction, immune system disorders, and even cancer.

[0003] However, the detection methods for fungal toxins mainly include laboratory techniques such as high performance liquid chromatography and liquid chromatography-mass spectrometry. Although they are highly accurate, they rely on expensive equipment, professional operation, and complex pretreatment, making it difficult to meet the needs of rapid screening at the grassroots level or on-site. Furthermore, they are not convenient for simultaneous detection of multiple test items. This phenomenon has become a problem that urgently needs to be solved by researchers in this field. Utility Model Content

[0004] The purpose of this invention is to provide a multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products, in order to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a multiplex fluorescent immunoassay test strip for mycotoxins in agricultural products, comprising an outer plate, wherein test strips are arranged in an array inside the outer plate, and a flow guide groove is provided at the center of the right end of each test strip, wherein the right end of the flow guide groove is connected to an absorbent pad.

[0006] This utility model further illustrates that: the test paper and the outer plate are fixed to each other, and the test paper is parallel to each other inside the outer plate.

[0007] The present invention further describes that: an observation groove is provided on the surface of the outer plate, the observation groove penetrates the surface of the outer plate, and a transparent film is provided between the observation groove and the test paper.

[0008] The present invention further describes that: a tail plate is fixedly provided at the right end of the outer plate, a buckle is provided on the left side of the tail plate near the outer plate, the buckles are symmetrically arranged on the front and rear sides of the tail plate, and a matching shell is provided on the outside of the tail plate.

[0009] The present invention further describes that: the tail plate is provided with a liquid inlet, the position of the liquid inlet and the test paper correspond to each other, the position of the absorbent pad and the liquid inlet correspond to each other, and a sealing block is provided between the absorbent pad and the liquid inlet, and the angle between the sealing block and the liquid inlet is °.

[0010] Compared with the prior art, the beneficial effects achieved by this utility model are: This utility model,

[0011] (1) By setting multiple sets of test strips, multiple test items can be detected in one go using the same sample inside the device. When the sample comes into contact with the test strip through the absorbent pad, the sample will flow to the left side of the test strip through the guide groove. This not only allows the sample to flow in the preset direction to avoid cross-contamination, but also optimizes the utilization rate of the sample.

[0012] (2) By setting the sample to first contact the sealing block and then the absorbent pad during the sample entry process, the absorbent pad has strong water absorption and will suck the sample in. Then, it will flow to the left through the guide channel and be detected. The sealing block has relatively weak water absorption. After a period of time, the sealing block absorbs water and expands, sealing the angle between the sealing block and the liquid inlet. This will block the gap between the liquid inlet and the absorbent pad and prevent external impurities from entering the test paper and causing sample contamination. At the same time, it can also prevent the sample that has been dripped in from flowing back out of the liquid inlet. Attached Figure Description

[0013] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is a schematic diagram of the cross-sectional structure of the casing of this utility model;

[0016] Figure 3 This is a schematic diagram of the cross-sectional structure of the outer panel of this utility model;

[0017] Figure 4 This is a cross-sectional view of the liquid inlet structure of this utility model;

[0018] Figure 5 This is a schematic diagram of the sealing block installation structure of this utility model;

[0019] In the diagram: 1. Outer plate; 2. Shell; 3. Observation slot; 4. Tail plate; 5. Buckle; 6. Test paper; 7. Flow guide channel; 8. Liquid inlet; 9. Sealing block; 10. Water-absorbing pad. Detailed Implementation

[0020] The following detailed, non-limiting description of the present invention, in conjunction with preferred embodiments and accompanying drawings, is provided. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0021] Example 1

[0022] A multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products, as shown in the figure, includes an outer plate 1. Test strips 6 are arranged in an array inside the outer plate 1. Each test strip 6 has a flow channel 7 at its right center, and the right end of the flow channel 7 is connected to an absorbent pad 10. Multiple sets of test strips 6 are arranged inside the outer plate 1 to detect various mycotoxins in agricultural products. Multiple test items can be detected simultaneously using the same sample. When the sample comes into contact with the test strip 6 through the absorbent pad 10, the sample is guided to the left side of the test strip 6 through the flow channel 7. This not only allows the sample to flow in a preset direction, avoiding cross-contamination, but also optimizes sample utilization.

[0023] The test strip 6 is fixed to the outer plate 1. The test strip 6 is parallel to each other inside the outer plate 1. The test strip 6 is fixed inside the outer plate 1, which can prevent the test strip 6 from falling or curling. The outer plate 1 can also protect the test strip 6 and improve the stability of the test strip 6 during use. The parallel arrangement of the test strip 6 inside the outer plate 1 can also prevent the samples from coming into contact and contaminating each other.

[0024] Example 2

[0025] Similar to Example 1, except that the outer plate 1 has an observation groove 3 on its surface, which penetrates the surface of the outer plate 1. A transparent film is provided between the observation groove 3 and the test paper 6. When in use, the observation groove 3 on the surface of the outer plate 1 can be used to observe the test results of the test paper 6 on the sample. At the same time, the transparent film between the outer plate 1 and the observation groove 3 will not cause the external environment to contaminate the sample without affecting the observation of the results.

[0026] Example 3

[0027] Similar to Example 1, except that a tail plate 4 is fixedly installed on the right end of the outer plate 1, and a buckle 5 is installed on the left side of the tail plate 4 near the outer plate 1. The buckles 5 are symmetrically arranged on the front and rear sides of the tail plate 4. A sleeve 2 of the same size is installed on the outside of the tail plate 4. The sleeve 2 can be installed on the outside of the tail plate 4 through the buckles 5, thereby protecting the tail plate 4 and preventing impurities in the external environment from entering the interior of the liquid inlet 8 and affecting the detection effect of the internal test strip 6.

[0028] Example 4

[0029] Similar to Example 1, the difference is that the tail plate 4 has an inlet 8 inside, and the inlet 8 is positioned opposite to the test strip 6. The absorbent pad 10 is also positioned opposite to the inlet 8. A sealing block 9 is positioned between the absorbent pad 10 and the inlet 8, and the angle between the sealing block 9 and the inlet 8 is 30°. In use, the sample is dripped into the device from the inlet 8. During the process, the sample will first come into contact with the sealing block 9 and then with the absorbent pad 10. The absorbent pad 10 has strong water absorption and will suck in the sample. The sample will then flow to the left through the guide channel 7 for testing. The sealing block 9 has relatively weak water absorption. After a period of time, the sealing block 9 absorbs water and expands, sealing the angle between the sealing block 9 and the inlet 8. This blocks the gap between the inlet 8 and the absorbent pad 10, preventing external impurities from entering the test strip 6 and causing sample contamination. It also prevents the already dripped sample from flowing back out from the inlet 8.

[0030] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products, comprising an outer plate (1), characterized in that: The outer plate (1) is internally arrayed with test strips (6), and each test strip (6) has a flow guide groove (7) at the center of its right end. The right end of the flow guide groove (7) is connected to the absorbent pad (10).

2. The multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products according to claim 1, characterized in that: The test paper (6) is fixed to the outer plate (1), and the test paper (6) is parallel to each other inside the outer plate (1).

3. The multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products according to claim 1, characterized in that: The outer plate (1) has an observation groove (3) on its surface. The observation groove (3) penetrates the surface of the outer plate (1). A transparent film is provided between the observation groove (3) and the test paper (6).

4. The multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products according to claim 1, characterized in that: A tail plate (4) is fixedly provided on the right end of the outer plate (1). A buckle (5) is provided on the left side of the tail plate (4) near the outer plate (1). The buckles (5) are symmetrically arranged on the front and rear sides of the tail plate (4). A matching shell (2) is provided on the outside of the tail plate (4).

5. The multiplex fluorescent immunoassay strip for detecting mycotoxins in agricultural products according to claim 4, characterized in that: The tail plate (4) is provided with an inlet (8) inside. The position of the inlet (8) corresponds to that of the test paper (6). The position of the absorbent pad (10) corresponds to that of the inlet (8). A sealing block (9) is provided between the absorbent pad (10) and the inlet (8). The angle between the sealing block (9) and the inlet (8) is 30°.

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