An intermittent immersion method cultivation device

Abstract

The application relates to the technical field of tissue culture, in particular to an intermittent immersion culture device, which comprises a coupling assembly, one side of the coupling assembly is threadedly sleeved with a first culture dish, and the other side of the coupling assembly is threadedly sleeved with a second culture dish; compared with a traditional large device for connecting various culture bottles by means of a pump, the coupling assembly is adopted to threadedly connect the first culture dish and the second culture dish, so that the structure is more compact, and the overall size is significantly reduced. Meanwhile, the simplified connection mode greatly simplifies the operation process, the complicated pipeline connection is not needed, when sterilization treatment is carried out, the complicated pipeline system does not need to be sterilized by means of a high-pressure sterilization pot like the traditional device, the operation difficulty and time cost are reduced, the requirement for an experimental site is no longer harsh, and the device is suitable for small laboratory and even household tissue culture scenes.

Description

Technical Field

[0001] This application relates to the field of tissue culture technology, and in particular to an intermittent immersion culture apparatus. Background Technology

[0002] In the field of tissue culture, traditional culture techniques face numerous challenges. Currently, some tissue culture devices use pumps to deliver sterile liquid tissue culture medium, allowing the entire plant to participate in nutrient absorption by immersing it in the medium. Compared to traditional solid culture media, this significantly accelerates plant growth. However, maintaining a sterile environment throughout the entire tissue culture process, from inoculation to culture, requires extremely high aseptic techniques, making the procedure extremely complex. Traditional devices that rely on pumps to connect individual culture flasks are not only bulky but also cumbersome to operate. Sterilization requires an autoclave to sterilize the complex piping and culture flask system, a complex and time-consuming process. Such devices also have stringent requirements for experimental facilities; tissue culture work is virtually impossible in small laboratories or environments lacking cleanrooms. In recent years, the application of intermittent immersion culture in tissue culture has gradually attracted attention, but existing related culture devices have significant shortcomings in structural design. For example, some devices use snap-fit ​​or complex connection structures instead of threaded connections to connect culture dishes, resulting in cumbersome installation and disassembly processes that are not only time-consuming and labor-intensive but also prone to damaging culture dishes or connecting components. Simultaneously, existing devices lack partition structures with mesh and auxiliary through-holes, making it difficult to achieve precise material exchange and effective separation between culture dishes, failing to create the specific environment suitable for intermittent immersion culture, and thus affecting culture results. Furthermore, traditional devices struggle to meet lightweight requirements, limiting their application in more scenarios. Therefore, there is an urgent need for a new type of tissue culture device that can achieve lightweight design, simplify operation procedures, and reduce the requirements for the experimental environment while ensuring culture results, making it suitable for tissue culture scenarios of different sizes, including laboratories and even home environments. Utility Model Content

[0003] To address the problems mentioned in the background section, this application provides an intermittent immersion culture apparatus.

[0004] This application provides an intermittent immersion culture device, which adopts the following technical solution: an intermittent immersion culture device includes a coupling component, a first culture dish is threadedly sleeved on one side of the coupling component, and a second culture dish is threadedly sleeved on the other side of the coupling component; The coupling assembly includes a partition for separating a first culture dish and a second culture dish, a mesh opening through the center of the partition, and a sleeve for threaded connection of the first culture dish and the second culture dish. The top surface of the partition is provided with a replaceable filter layer, and a self-tapping screw positioning hole is provided in the center of the mesh opening.

[0005] The above scheme achieves controllable liquid permeation and isolation through a multi-layer functional structure design. The replaceable filter layer can adapt to different experimental needs, improving the reusability and compatibility of the device.

[0006] Optionally, the top and bottom edges of the partition are provided with annular sealing rings, the outer surface of the sleeve is provided with an anti-slip structure, and the inner wall of the sleeve is provided with a threaded structure that mates with the first and second culture dishes.

[0007] The above-mentioned solution effectively prevents liquid leakage through the double sealing ring design, and the anti-slip structure combined with the precision threaded interface enhances operational stability and ensures the sealing reliability of the device during dynamic immersion.

[0008] Optionally, the replaceable filter layer includes a flat filter screen fixed to the top of the partition by adhesive bonding, wherein the mesh size of the flat filter screen is in the range of 50-300 mesh.

[0009] The above solution utilizes an adhesive planar filter for rapid replacement, and different mesh sizes can be selected to meet the needs of cell or microbial culture, optimizing liquid exchange efficiency and substance interception effect.

[0010] Optionally, the anti-slip structure includes raised textures extending along the sleeve axial direction, the height of the raised textures being 0.5-2mm and the spacing between adjacent raised textures being 1-3mm.

[0011] The above solution improves hand grip friction through a reasonable layout of axial raised textures, reduces the risk of slippage during operation, and maintains structural simplicity and ease of processing.

[0012] Optionally, the material of the annular sealing ring is selected from any one of silicone, rubber or thermoplastic elastomer, and its cross-sectional shape is circular or trapezoidal.

[0013] The above solution utilizes elastic materials and cross-sectional shape design to achieve adaptive sealing, maintaining deformation recovery capability during repeated disassembly and reassembly, and extending the service life of the sealing components.

[0014] Optionally, the partition is a circular or polygonal structure, and its diameter or diagonal length is 1-5 mm larger than the opening diameter of the first culture dish and the second culture dish.

[0015] The above solution uses a partition slightly larger than the opening of the culture dish to form an edge limiting structure, which avoids assembly misalignment and ensures that the sealing ring is evenly compressed, thereby improving the overall assembly accuracy.

[0016] Optionally, at least three auxiliary through holes are provided around the mesh, and the auxiliary through holes are distributed in a ring array with the mesh as the center.

[0017] The above scheme utilizes the synergistic effect of auxiliary through holes and central mesh to optimize the distribution of liquid flow paths, reduce local pressure differences, and ensure the uniformity and controllability of the immersion process.

[0018] Optionally, the opening ends of the first and second culture dishes are provided with threaded interfaces that mate with the sleeves, wherein the thread pitch of the threaded interfaces is 1-3 mm and the thread depth is 0.5-1.5 mm.

[0019] The above solution enables modular expansion capabilities through standardized threaded interface design, allowing for rapid docking with petri dishes of different sizes, and reducing system construction complexity and maintenance costs.

[0020] In summary, this application includes the following beneficial technical effects: Lightweight and Simplified Operation: Compared to the bulky traditional devices that rely on pumps to connect various culture flasks, this application uses a threaded coupling assembly to connect the first and second culture dishes, resulting in a more compact structure and a significantly reduced overall size. Simultaneously, the simplified connection method greatly simplifies the operation process, eliminating the need for complex piping connections. During sterilization, it also eliminates the need for autoclaving complex piping systems as in traditional devices, reducing operational difficulty and time costs. It also lessens the requirements for experimental sites, making it suitable for small laboratories and even home tissue culture scenarios. Convenient installation and disassembly, and high durability: The threaded connection method for connecting the culture dish makes installation and disassembly much easier compared to snap-fit ​​or complex connection structures. This not only saves time and effort but also effectively prevents damage to the culture dish or connecting parts due to improper operation. Furthermore, the annular sealing ring is made of elastic materials such as silicone, rubber, or thermoplastic elastomers, with a circular or trapezoidal cross-sectional shape. This ensures good deformation recovery during repeated disassembly and reassembly, extending the service life of the sealing component and guaranteeing the durability of the device. Precise material exchange and effective separation: The partition in the coupling component is equipped with mesh and auxiliary through holes. The mesh is used to separate the first culture dish and the second culture dish, and the auxiliary through holes are distributed in a ring array with the mesh as the center. The two work together to optimize the distribution of liquid flow path, reduce local pressure differences, and enable precise material exchange between culture dishes. At the same time, they effectively separate different culture environments, create a specific environment suitable for intermittent immersion culture, and thus improve the culture effect. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the device in the embodiments of this application; Figure 2 This is a schematic diagram of a partial structure of the device in an embodiment of this application; Figure 3 This is a schematic diagram of a partial structure of the coupling component in an embodiment of this application; Figure 4 This is a schematic diagram of the partial structure installation of the coupling component in an embodiment of this application; Figure 5 This is a schematic diagram of the self-tapping screw installation position in an embodiment of this application; Reference numerals: 1. First culture dish; 2. Coupling assembly; 201. Partition; 202. Sleeve; 203. Mesh; 204. Sealing ring; 205. Self-tapping screw positioning hole; 3. Second culture dish. Detailed Implementation

[0022] The following is in conjunction with the appendix Figure 1-4 This application will be described in further detail.

[0023] This application discloses an intermittent immersion culture device.

[0024] Please see Figure 1 An intermittent immersion culture device includes a coupling component 2, with a first culture dish 1 threadedly connected to one side of the coupling component 2 and a second culture dish 3 threadedly connected to the other side of the coupling component 2. Please see Figures 2 to 4 The coupling component 2 includes a partition 201 for separating the first culture dish 1 and the second culture dish 3, a mesh 203 through which the mesh is opened in the center of the partition 201, and a sleeve 202 for threadedly connecting the first culture dish 1 and the second culture dish 3. The top surface of the partition 201 is provided with a replaceable filter layer.

[0025] The top and bottom edges of the partition 201 are provided with annular sealing rings 204, the outer surface of the sleeve 202 is provided with an anti-slip structure, and the inner wall of the sleeve 202 is provided with a threaded structure that mates with the first culture dish 1 and the second culture dish 3.

[0026] The partition 201 is a circular or polygonal structure, and its diameter or diagonal length is 1-5 mm larger than the opening diameter of the first culture dish 1 and the second culture dish 3.

[0027] The opening ends of the first culture dish 1 and the second culture dish 3 are provided with threaded interfaces that mate with the sleeve 202. The thread pitch of the threaded interface is 1-3mm and the thread depth is 0.5-1.5mm.

[0028] The replaceable filter layer includes a flat filter screen that is fixed to the top of the partition 201 by adhesive bonding. The mesh size of the flat filter screen ranges from 50 to 300 mesh.

[0029] The anti-slip structure includes raised textures extending along the axial direction of the sleeve 202. The height of the raised textures is 0.5-2mm, and the spacing between adjacent raised textures is 1-3mm.

[0030] The material of the annular sealing ring 204 is selected from any one of silicone, rubber or thermoplastic elastomer, and its cross-sectional shape is circular or trapezoidal.

[0031] At least three auxiliary through holes are provided around the mesh 203, and the auxiliary through holes are distributed in a ring array with the mesh 203 as the center.

[0032] Further explanation is needed: Coupling component 2 plays a crucial role in the intermittent immersion culture device. The partition 201 separates the first culture dish 1 and the second culture dish 3, forming relatively independent culture spaces. At the same time, the mesh 203 and auxiliary through holes on it allow for the exchange and flow of substances and gases between the two culture dishes while maintaining separation, meeting specific needs during the culture process. The sleeve 202 is tightly fitted to the first culture dish 1 and the second culture dish 3 through a threaded structure, firmly connecting them into a whole, ensuring the structural stability of the device, facilitating operation and movement. The anti-slip structure on its outer surface enhances the friction when the operator holds the device, preventing it from slipping during operation. The replaceable filter layer on the top of the partition 201 can filter the exchanged substances, blocking impurities, ensuring that the substances entering the culture dishes meet the culture requirements. The annular sealing ring 204 at the edge of the partition 201 can effectively prevent liquid leakage and gas leakage from the culture dishes, maintaining the sealing and stability of the culture environment.

[0033] The implementation principle of the intermittent immersion culture device in this application embodiment is as follows: First, in the culture preparation stage, the first culture dish 1 and the second culture dish 3 are tightly connected by the coupling component 2 through the threaded structure of the sleeve 202. The sealing ring 204 on the edge of the partition 201 ensures that a sealed space is formed between the two culture dishes. A replaceable filter layer is installed on the top of the partition 201. A filter with appropriate filtration precision is selected according to the culture requirements. Then, culture medium and culture are added to the two culture dishes respectively, laying the foundation for the culture process.

[0034] Secondly, regarding spatial separation and material exchange, the partition 201 separates the first culture dish 1 and the second culture dish 3 into independent areas. However, the mesh 203 in the center and the surrounding auxiliary through holes allow the two areas to maintain the flow of liquids, gases and other substances while being physically separated. The filter layer screens the flowing substances, blocking impurities from entering the target culture area and ensuring a pure culture environment.

[0035] Next, intermittent immersion is achieved. When the culture needs to be immersed, external operations such as tilting or lifting devices are used to allow the culture solution in the first culture dish 1 to seep into the second culture dish 3 through the mesh 203 and filter layer, thus immersing the culture. After the set time is reached, the liquid is returned by reverse operation, realizing the periodic immersion and exposure of the culture, simulating the intermittent nutrient supply in the natural growth environment.

[0036] Next, structural stability and operational assurance are ensured. The anti-slip structure on the outer surface of the sleeve 202 provides operators with a stable grip, facilitating precise control of the device's angle or position during liquid exchange. The threaded connection and sealing ring 204 work together to prevent liquid leakage or external contamination, maintaining the sealing and stability of the culture environment and avoiding cross-contamination or environmental interference.

[0037] Finally, after the culture is completed and the culture cycle is finished, the two culture dishes can be easily disassembled through the threaded structure of the sleeve 202, the filter layer on the partition 201 can be replaced and cleaned, and all components can be cleaned and disinfected to prepare for the next culture. Throughout the process, the separation, connection, sealing and filtration functions of the coupling component 2 work together to achieve efficient and controllable intermittent immersion culture.

[0038] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. An intermittently submerged culture device comprising a coupling assembly (2), characterized in that: The first culture dish (1) is threaded onto one side of the coupling component (2), and the second culture dish (3) is threaded onto the other side of the coupling component (2). The coupling component (2) includes a partition (201) for separating the first culture dish (1) and the second culture dish (3), a mesh (203) through the center of the partition (201), and a sleeve (202) for threaded connection of the first culture dish (1) and the second culture dish (3). The top surface of the partition (201) is provided with a replaceable filter layer, and a self-tapping screw positioning hole (205) is provided in the center of the mesh (203).

2. The device for culturing cells according to claim 1, wherein: The top and bottom edges of the partition (201) are provided with annular sealing rings (204), the outer surface of the sleeve (202) is provided with an anti-slip structure, and the inner wall of the sleeve (202) is provided with a threaded structure that cooperates with the first culture dish (1) and the second culture dish (3).

3. The device for culturing cells according to claim 1, wherein: The replaceable filter layer includes a flat filter screen that is fixed to the top of the partition (201) by adhesive bonding, wherein the mesh size of the flat filter screen is in the range of 50-300 mesh.

4. The device for culturing cells according to claim 2, wherein: The anti-slip structure includes raised textures extending along the axial direction of the sleeve (202), the height of the raised textures being 0.5-2mm, and the spacing between adjacent raised textures being 1-3mm.

5. The device for culturing cells according to claim 2, wherein: The material of the annular sealing ring (204) is selected from any one of silicone, rubber or thermoplastic elastomer, and its cross-sectional shape is circular or trapezoidal.

6. The device of claim 1, wherein: The partition (201) is a circular or polygonal structure, and its diameter or diagonal length is 1-5 mm larger than the opening diameter of the first culture dish (1) and the second culture dish (3).

7. The device of claim 1, wherein: At least three auxiliary through holes are provided around the mesh (203), and the auxiliary through holes are distributed in a ring array with the mesh (203) as the center.

8. The device of claim 1, wherein: The first culture dish (1) and the second culture dish (3) have threaded interfaces at their open ends that mate with the sleeve (202). The thread pitch of the threaded interface is 1-3 mm and the thread depth is 0.5-1.5 mm.

Images