A protein purification filter device for efficiently separating impurities

Abstract

The utility model relates to a kind of protein purification filtering devices of high efficiency separation impurity, belong to protein purification technical field.The protein purification filtering device of high efficiency separation impurity, comprising: purification mechanism, purification mechanism includes workbench, purification bucket body, bucket cover and centrifugal component, and purification bucket body is fixedly arranged on the upper surface of workbench;Cleaning mechanism, cleaning mechanism includes connecting rod and lifting assembly;Filtering mechanism, filtering mechanism includes filter bottom cover, outer filter membrane, flow guide net, middle filter membrane and inner filter membrane;By rotatingly setting filter bottom cover in the inside of purification bucket body, and setting outer filter membrane on the surface of filter bottom cover, setting middle filter membrane and inner filter membrane in the inside of outer filter membrane, and the aperture of outer filter membrane, middle filter membrane and inner filter membrane is gradiently decreased from inside to outside, centrifugal component drives filter bottom cover to rotate, to realize full-scale efficient separation to impurity, avoid filter membrane hole to be blocked or the purity of protein solution after filtration is insufficient, and then improve the filtering efficiency of protein purification.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, and in particular to a protein purification filtration device that efficiently separates impurities. Background Technology

[0002] In biopharmaceuticals, enzyme engineering, and scientific research, protein purification is a crucial step in obtaining high-purity target proteins. Filtration, as the core separation method in the protein purification process, directly determines the quality, activity, and production cost of the subsequent protein products. Currently, protein purification filtration devices widely used in industry and laboratories mostly use single-pore-size filter membranes as the core separation unit.

[0003] As shown in the reference case "A Filtration Device for Protein Purification and Separation" (publication number CN221788450U), the operator pours the protein into the filter cartridge. Then, the operator starts the second motor to drive the transmission rod to rotate along the inside of the sealed shaft. When the transmission rod rotates, it drives the third sprocket to rotate. When the third sprocket rotates, it drives the fourth sprocket to rotate through the second chain. When the fourth sprocket rotates, it drives the rotating column to rotate along the shaft seat. When the rotating column rotates, it drives the filter cartridge to rotate, thereby filtering the protein through centrifugal force. According to the above references, the aforementioned device uses a single-pore size filter membrane as the core separation unit. Because the crude protein extract contains impurities of various sizes, if a filter membrane with a smaller pore size is selected, large impurities in the crude protein extract will quickly accumulate on the membrane surface, causing blockage of the membrane pores. If a filter membrane with a larger pore size is selected, because the pore size is much larger than the impurity size, it cannot effectively retain the impurities, resulting in insufficient purity of the filtered protein solution. Furthermore, because the retention range of a single-pore size filter membrane is fixed, it cannot achieve efficient separation of impurities of all sizes, requiring multiple subsequent filtrations, leading to low filtration efficiency. Utility Model Content

[0004] Therefore, it is necessary to provide a protein purification filtration device that can efficiently separate impurities across all scales, addressing the problem that the fixed retention range of single-pore size filter membranes cannot achieve efficient separation of impurities.

[0005] A protein purification and filtration device for high-efficiency separation of impurities includes: a purification mechanism, wherein the purification mechanism includes a workbench, a purification barrel, a barrel cover and a centrifugation assembly, and the purification barrel is fixedly disposed on the upper surface of the workbench; A cleaning mechanism, comprising a connecting rod and a lifting assembly for driving the connecting rod to rise and fall; The filtration mechanism includes a filter bottom cover, an outer filter membrane, a flow guide net, a middle filter membrane, and an inner filter membrane. The filter bottom cover is rotatably disposed inside the purification tank. The outer filter membrane, the middle filter membrane, and the inner filter membrane are all fixedly disposed on the upper surface of the filter bottom cover. The flow guide net is disposed between the outer filter membrane, the middle filter membrane, and the inner filter membrane.

[0006] In one embodiment, the upper surface of the filter cover is provided with an inner bottom plate, a middle bottom plate and an outer bottom plate, the inner bottom plate, the middle bottom plate and the outer bottom plate do not contact each other, the inner bottom plate is located inside the inner filter membrane, the middle bottom plate is located between the middle filter membrane and the inner filter membrane, and the outer bottom plate is located between the outer filter membrane and the middle filter membrane.

[0007] In one embodiment, the flow guide net is provided in two sets, which are respectively located between the outer filter membrane and the middle filter membrane and between the middle filter membrane and the inner filter membrane, and are respectively fixedly connected to the middle bottom plate and the outer bottom plate.

[0008] In one embodiment, the inner filter membrane is located inside the middle filter membrane, the middle filter membrane is located inside the outer filter membrane, and the pore sizes of the outer filter membrane, the middle filter membrane, and the inner filter membrane decrease in a gradient from the inside to the outside.

[0009] In one embodiment, the upper surfaces of the outer filter membrane and the middle filter membrane are provided with filter top covers, and the filter top covers are fixedly disposed on the upper surface of the two sets of guide nets.

[0010] In one embodiment, the filter top cover has a liquid inlet on its surface, and a liquid blocking plate corresponding to the liquid inlet is snapped into the inner side of the filter top cover, and the liquid blocking plate is slidably disposed on the outer arc surface of the connecting rod.

[0011] In one embodiment, the lifting assembly includes a mounting frame, a motor, a connecting block, and a threaded rod. The mounting frame is fixedly disposed on the upper surface of the worktable, the motor is bolted to the top of the mounting frame, and the threaded rod is rotatably disposed inside the mounting frame.

[0012] In one embodiment, the connecting block is threaded onto the outer arc surface of the threaded rod, and one end of the connecting rod is rotatably connected to the connecting block, while the other end of the connecting rod is connected to the guide net and the inner bottom plate respectively.

[0013] Beneficial effects 1. The above-mentioned protein purification filtration device, by rotating the filter bottom cover inside the purification tank, and setting an outer filter membrane on the surface of the filter bottom cover, and setting a middle filter membrane and an inner filter membrane inside the outer filter membrane, with the pore size of the outer filter membrane, middle filter membrane and inner filter membrane decreasing in a gradient from the inside to the outside, and the centrifugation component driving the filter bottom cover to rotate, can achieve efficient separation of impurities at all scales, avoid the filter membrane pores being blocked or the purity of the filtered protein solution being insufficient, thereby improving the filtration efficiency of protein purification.

[0014] 2. The above-mentioned protein purification filtration device, by setting a flow guide net between the outer filter membrane and the middle filter membrane, and between the middle filter membrane and the inner filter membrane, and setting the flow guide net to be honeycomb, avoids the filter membrane from being pressed and stuck together, thereby preventing channel blockage. At the same time, it guides the fluid to be evenly distributed, reducing protein shear denaturation caused by excessively high local flow rates. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the external filter membrane lifting structure of this utility model; Figure 3 This is an exploded view of the overall structure of this utility model; Figure 4 This is a cross-sectional view of the purification tank structure of this utility model; Figure 5 This utility model Figure 4 Enlarged view of point A in the middle; Figure 6 This utility model Figure 4 Front view.

[0017] Reference numerals: 100, Purification mechanism; 200, Cleaning mechanism; 300, Filtration mechanism; 101, Workbench; 102, Purification tank body; 103, Tank lid; 104, Centrifuge assembly; 201, Mounting frame; 202, Motor; 203, Connecting block; 204, Connecting rod; 205, Threaded rod; 301, Filter top cover; 302, Filter bottom cover; 303, External filter membrane; 304, Flow guide net; 305, Middle filter membrane; 306, Internal filter membrane; 307, Inner bottom plate; 308, Middle bottom plate; 309, External bottom plate. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0019] The following is combined withFigures 1-6 This invention describes a highly efficient protein purification and filtration device for separating impurities.

[0020] In one embodiment, a protein purification and filtration device for efficient separation of impurities includes: a purification mechanism 100, which includes a workbench 101, a purification tank 102, a tank cover 103, and a centrifugation assembly 104, wherein the purification tank 102 is fixedly disposed on the upper surface of the workbench 101; and a filtration mechanism 300, which includes a filter bottom cover 302, an outer filter membrane 303, a flow guide net 304, a middle filter membrane 305, and an inner filter membrane 306. 302 is rotatably installed inside the purification tank 102. The outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306 are all fixedly installed on the upper surface of the filter bottom cover 302. The guide net 304 is installed between the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306. The inner filter membrane 306 is located inside the middle filter membrane 305, and the middle filter membrane 305 is located inside the outer filter membrane 303. The pore size of the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306 decreases in a gradient from the inside to the outside. In this embodiment, at the start of filtration, the lid 103 is bolted to the upper surface of the purification tank 102. Then, the centrifuge assembly 104 drives the rotating column through the purification tank 102 and rotates the filter bottom cover 302. The rotating column is sealed through the purification tank 102 to prevent liquid leakage through the interface. The filter bottom cover 302 drives the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306 on its surface to rotate. The inner filter membrane 306 traps cell debris and large-sized colloids, the middle filter membrane 305 traps impurities, and the outer filter membrane 303 is an ultrafiltration membrane to further trap small molecule impurities. The edges of each filter membrane are sealed by ultrasonic welding to form a stepped impurity trapping channel, thereby achieving efficient separation of impurities at all scales, avoiding clogging of the filter membrane pores or insufficient purity of the filtered protein solution, and thus improving the filtration efficiency of protein purification.

[0021] like Figure 3 , Figure 4 and Figure 5 As shown, there are two sets of flow guide nets 304, which are located between the outer filter membrane 303 and the middle filter membrane 305, and between the middle filter membrane 305 and the inner filter membrane 306, respectively. The two sets of flow guide nets 304 are fixedly connected to the middle bottom plate 308 and the outer bottom plate 309, respectively. The upper surfaces of the outer filter membrane 303 and the middle filter membrane 305 are provided with filter top covers 301, and the filter top covers 301 are fixedly installed on the upper surfaces of the two sets of flow guide nets 304. The surface of the filter top cover 301 is provided with a liquid inlet, and the inner side of the filter top cover 301 is fitted with a liquid blocking plate corresponding to the liquid inlet, and the liquid blocking plate is slidably installed on the outer arc surface of the connecting rod 204. In this embodiment, an inlet is provided to ensure that liquid can be injected into the inner filter membrane 306. Then, a liquid blocking plate is detachably installed inside the filter top cover 301 to block the inlet and prevent liquid from being thrown out of the inlet during centrifugation. By setting a flow guide net 304 between the outer filter membrane 303 and the middle filter membrane 305, and between the middle filter membrane 305 and the inner filter membrane 306, and setting the flow guide net 304 to be honeycomb, the filter membrane is prevented from being pressed and stuck together, which would cause the channel to be blocked. At the same time, the fluid is guided to be evenly distributed, reducing protein shear denaturation caused by excessive local flow rate.

[0022] like Figure 1 , Figure 2 and Figure 3 As shown, the cleaning mechanism 200 includes a connecting rod 204 and a lifting assembly for driving the connecting rod 204 to rise and fall; the upper surface of the filter bottom cover 302 is provided with an inner bottom plate 307, a middle bottom plate 308, and an outer bottom plate 309. The inner bottom plate 307, the middle bottom plate 308, and the outer bottom plate 309 do not contact each other, and the inner bottom plate 307 is located inside the inner filter membrane 306, the middle bottom plate 308 is located between the middle filter membrane 305 and the inner filter membrane 306, and the outer bottom plate 309 is located between the outer filter membrane 303 and the middle filter membrane 306. Between 05 and 05; the lifting assembly includes a mounting frame 201, a motor 202, a connecting block 203, and a threaded rod 205. The mounting frame 201 is fixedly installed on the upper surface of the workbench 101, the motor 202 is bolted to the top of the mounting frame 201, and the threaded rod 205 is rotatably installed inside the mounting frame 201; the connecting block 203 is threadedly sleeved on the outer arc surface of the threaded rod 205, and one end of the connecting rod 204 is rotatably connected to the connecting block 203, and the other end of the connecting rod 204 is connected to the guide net 304 and the inner bottom plate 307 respectively; In this embodiment, when it is necessary to clean the impurities inside the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306, the motor 202 is started to drive the threaded rod 205 to rotate. The threaded rod 205 drives the connecting block 203 to rise through the thread. One side of the connecting block 203 is set as a convex block and is locked into the mounting frame 201, thereby ensuring the stability of the connecting block 203 during movement. Then, the connecting rod 204 is driven to rise through the connecting block 203, and the inner bottom plate 307 is driven to rise through the connecting rod 204. Then, the middle bottom plate 308 and the outer bottom plate 309 are driven to rise through the guide net 304 and moved out from inside the purification tank 102, thereby facilitating the cleaning of impurities inside the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306 by the staff.

[0023] Working principle: Liquid is introduced into the inner filter membrane 306 through the inlet, and then the blocking plate and the barrel cover 103 are sequentially covered. Then, the centrifugal assembly 104 drives the filter bottom cover 302 to rotate inside the purification barrel 102. The filter bottom cover 302 drives the outer filter membrane 303, the middle filter membrane 305, and the inner filter membrane 306 on the surface to rotate synchronously. Because the connecting rod 204 is rotatably connected to the connecting block 203, the inner bottom plate 307, the middle bottom plate 308, and the outer bottom plate 309, the outer filter membrane 306 is kept in place. 3. The stability of the middle filter membrane 305 and the inner filter membrane 306 during rotation is ensured. Cell debris and large-sized colloids are then retained by the inner filter membrane 306, while impurities and proteins are retained by the middle filter membrane 305. The outer filter membrane 303 is an ultrafiltration membrane, which further retains small molecule impurities. The edges of each filter membrane are sealed by ultrasonic welding to form a stepped impurity retention channel, thereby achieving efficient separation of impurities across the entire scale. This avoids clogging of the filter membrane pores or insufficient purity of the filtered protein solution, thus improving the filtration efficiency of protein purification.

[0024] It should be noted that the motor 202 and other components mentioned above are all devices with relatively mature existing technologies. The specific model can be selected according to actual needs. At the same time, the motor 202 can be powered by an internal power supply or by AC power. The specific power supply method should be selected according to the situation, which will not be elaborated here.

[0025] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A highly efficient protein purification and filtration device for separating impurities, characterized in that, include: Purification mechanism (100) includes a workbench (101), a purification barrel (102), a barrel cover (103), and a centrifugation assembly (104). The purification barrel (102) is fixedly disposed on the upper surface of the workbench (101). Cleaning mechanism (200), the cleaning mechanism (200) includes a connecting rod (204) and a lifting assembly for driving the connecting rod (204) to rise and fall; The filtration mechanism (300) includes a filter bottom cover (302), an outer filter membrane (303), a guide net (304), a middle filter membrane (305), and an inner filter membrane (306). The filter bottom cover (302) is rotatably disposed inside the purification tank (102). The outer filter membrane (303), the middle filter membrane (305), and the inner filter membrane (306) are all fixedly disposed on the upper surface of the filter bottom cover (302). The guide net (304) is disposed between the outer filter membrane (303), the middle filter membrane (305), and the inner filter membrane (306).

2. The protein purification and filtration device for high-efficiency separation of impurities according to claim 1, characterized in that, The filter bottom cover (302) is provided with an inner bottom plate (307), a middle bottom plate (308) and an outer bottom plate (309) on its upper surface. The inner bottom plate (307), the middle bottom plate (308) and the outer bottom plate (309) do not contact each other. The inner bottom plate (307) is located inside the inner filter membrane (306), the middle bottom plate (308) is located between the middle filter membrane (305) and the inner filter membrane (306), and the outer bottom plate (309) is located between the outer filter membrane (303) and the middle filter membrane (305).

3. The protein purification and filtration device for high-efficiency separation of impurities according to claim 2, characterized in that, The flow guide net (304) is provided in two sets. The two sets of flow guide nets (304) are respectively located between the outer filter membrane (303) and the middle filter membrane (305) and between the middle filter membrane (305) and the inner filter membrane (306). The two sets of flow guide nets (304) are respectively fixedly connected to the middle bottom plate (308) and the outer bottom plate (309).

4. The protein purification and filtration device for high-efficiency separation of impurities according to claim 1, characterized in that, The inner filter membrane (306) is located inside the middle filter membrane (305), the middle filter membrane (305) is located inside the outer filter membrane (303), and the pore size of the outer filter membrane (303), the middle filter membrane (305) and the inner filter membrane (306) decreases in a gradient from the inside to the outside.

5. The protein purification and filtration device for high-efficiency separation of impurities according to claim 1, characterized in that, The upper surfaces of the outer filter membrane (303) and the middle filter membrane (305) are provided with filter top covers (301), and the filter top covers (301) are fixedly disposed on the upper surfaces of the two sets of guide nets (304).

6. The protein purification and filtration device for high-efficiency separation of impurities according to claim 5, characterized in that, The filter top cover (301) has an inlet port on its surface, and a blocking plate corresponding to the inlet port is snapped into the inner side of the filter top cover (301), and the blocking plate is slidably disposed on the outer arc surface of the connecting rod (204).

7. The protein purification and filtration device for high-efficiency separation of impurities according to claim 2, characterized in that, The lifting assembly includes a mounting frame (201), a motor (202), a connecting block (203), and a threaded rod (205). The mounting frame (201) is fixedly mounted on the upper surface of the workbench (101), the motor (202) is bolted to the top of the mounting frame (201), and the threaded rod (205) is rotatably mounted inside the mounting frame (201).

8. The protein purification and filtration device for high-efficiency separation of impurities according to claim 7, characterized in that, The connecting block (203) is threaded onto the outer arc surface of the threaded rod (205), and one end of the connecting rod (204) is rotatably connected to the connecting block (203), while the other end of the connecting rod (204) is connected to the guide net (304) and the inner bottom plate (307) respectively.

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