A magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection

Abstract

This invention discloses a magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection, comprising: a substrate, on which a mixing chamber, a reaction chamber, and a luminescence detection chamber are sequentially arranged; a feeding chamber is also provided on the substrate, and a feeding channel is provided between the feeding chamber and the mixing chamber; a sample loading plate, which is movably mounted on the substrate, and has a feeding port that corresponds one-to-one with the feeding chamber; a liquid injection port on the sample loading plate, which corresponds to the luminescence detection chamber; and a flexible sheet, which is located between the substrate and the sample loading plate and is mounted on the sample loading plate. This invention reduces manual intervention, enables rapid detection of whole blood samples, and facilitates subsequent cleaning after detection.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, specifically to a magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection. Background Technology

[0002] Whole blood sample testing is widely used in clinical diagnosis, offering advantages such as rapid and accurate disease diagnosis. However, traditional whole blood testing methods suffer from problems such as complex operation, long testing time, and low automation. Magnetic microparticle chemiluminescence microfluidic chip technology combines microfluidic technology and chemiluminescence detection, representing a novel testing method in the field of whole blood testing. Nevertheless, existing magnetic microparticle chemiluminescence microfluidic chips have drawbacks such as inconvenience in cleaning after use. Utility Model Content

[0003] To address the aforementioned technical shortcomings, the purpose of this invention is to provide a magnetic microparticle chemiluminescence microfluidic chip for whole blood sample testing, which is easy to clean after use.

[0004] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: The present invention provides a magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection, comprising: a substrate, on which a mixing chamber, a reaction chamber, and a luminescence detection chamber are sequentially arranged; a feeding chamber is also arranged on the substrate, and a feeding channel is arranged between the feeding chamber and the mixing chamber; a sample loading plate, which is movably disposed on the substrate; a feeding port is provided on the sample loading plate, which is arranged in a one-to-one correspondence with the feeding chamber; a liquid injection port is also provided on the sample loading plate, which corresponds to the luminescence detection chamber; and a flexible sheet, which is located between the substrate and the sample loading plate and is disposed on the sample loading plate; wherein, the substrate is provided with two slots, one of which is located between the mixing chamber and the reaction chamber, and is connected to the mixing chamber by a first channel and to the reaction chamber by a second channel; the other slot is located between the reaction chamber and the luminescence detection chamber, and is connected to the reaction chamber and the luminescence detection chamber through a third channel and a fourth channel, respectively.

[0005] Furthermore, it also includes trapezoidal protrusions. Two trapezoidal protrusions are provided on the flexible sheet, and the two trapezoidal protrusions are fitted together in two slots.

[0006] Furthermore, the template is provided with two receiving cavities, which correspond to two trapezoidal protrusions.

[0007] Furthermore, the sample plate has two connection ports, which are connected to two receiving cavities respectively, and are used to connect to an external air pump.

[0008] Furthermore, the flexible sheet is provided with a first through hole, which corresponds to the feed inlet. The flexible sheet is also provided with a second through hole, which corresponds to the liquid injection port.

[0009] Furthermore, a fixing seat is provided at one end of the substrate, and a rotating shaft is rotatably mounted on the fixing seat. The end of the sample plate is fixedly sleeved on the outside of the rotating shaft.

[0010] Furthermore, magnetic particle-labeled ligands are placed inside the reaction chamber.

[0011] Furthermore, two iron sheets are provided on the substrate, and two magnetic blocks are provided on the sample plate, with the two magnetic blocks and the two iron sheets arranged in a one-to-one correspondence.

[0012] Furthermore, a feed pipe is provided at the feed inlet.

[0013] Furthermore, an injection tube is provided on the injection port.

[0014] The beneficial effects of this utility model are as follows: This utility model uses a trapezoidal protrusion and a slot to cooperate and an external air pump to facilitate the control of liquid flow into the reaction chamber and the light-emitting detection chamber; at the same time, due to the setting of a rotating shaft, the sample plate can be rotated and the sample plate can be fixed by magnetic adsorption of iron sheet. After use, the sample plate can be easily cleaned by rotating it. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0017] Figure 2 This is a schematic diagram of the overall cross-sectional structure of this utility model.

[0018] Figure 3 This is a schematic diagram of the substrate structure of this utility model.

[0019] Figure 4 for Figure 3 Enlarged schematic diagram of region A in the middle.

[0020] Figure 5 This is a schematic diagram of the flexible sheet structure of this utility model.

[0021] Figure 6 This is a schematic diagram showing the state of the sample plate being rotated and the substrate being separated according to this utility model.

[0022] Explanation of reference numerals in the attached drawings: 1. Substrate; 11. Feeding chamber; 111. Feeding channel; 12. Mixing chamber; 13. Reaction chamber; 14. Light emission detection chamber; 15. First channel; 16. Second channel; 17. Third channel; 18. Fourth channel; 19. Slot; 2. Sample loading plate; 21. Feed inlet; 22. Liquid injection port; 23. Connection port; 24. Receiving cavity; 3. Flexible sheet; 31. Trapezoidal protrusion; 32. First through hole; 33. Second through hole; 4. Fixing base; 41. Rotating shaft; 5. Feeding pipe; 6. Liquid injection pipe; 7. Magnetic block; 8. Iron sheet. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-5 This invention provides a magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection, comprising a substrate 1, on which a mixing chamber 12, a reaction chamber 13, and a light-emitting detection chamber 14 are sequentially arranged. The substrate 1 also has two feeding chambers 11 located on one side of the mixing chamber 12, and each feeding chamber 11 is provided with a feeding channel 111 between the mixing chamber 12 and the mixing chamber 12. A slot 19 is provided between the mixing chamber 12 and the reaction chamber 13. A first channel 15 is connected between the slot 19 and the mixing chamber 12, and a second channel 16 is connected between the slot 19 and the reaction chamber 13.

[0025] A slot 19 is also provided between the reaction chamber 13 and the light emission detection chamber 14. The slot 19 is connected to the reaction chamber 13 through the third channel 17 and to the light emission detection chamber 14 through the fourth channel 18.

[0026] Two feed ports 21 are provided on the substrate 1, and the two feed ports 21 correspond to the two mixing chambers 12 respectively; the substrate 1 is also provided with a liquid injection port 22, and the liquid injection port 22 is arranged in a one-to-one correspondence with the light emission detection chamber 14.

[0027] The chip also includes a sample plate 2, and a flexible sheet 3 is disposed between the sample plate 2 and the substrate 1. The flexible sheet 3 is made of an elastic material, such as silicone, to give it good elasticity. The elasticity of the flexible sheet 3 helps to improve the sealing effect between the flexible sheet 3 and the substrate 1. Two first through holes 32 and one second through hole 33 are disposed through the flexible sheet 3. The two first through holes 32 correspond to the two feed ports 21 respectively, and the second through hole 33 corresponds to the liquid injection port 22. The flexible sheet 3 is disposed on the sample plate 2. Specifically, the edges of the flexible sheet 3 and the edges of the first through holes 32 and the second through holes 33 can be fixed to the sample plate 2 by means of adhesive or the like.

[0028] A slot 19 is also provided between the mixing chamber 12 and the reflective detection chamber. The slot 19 is connected to the reaction chamber 13 by a third channel 17, and the slot 19 is connected to the light-emitting detection chamber 14 by a fourth channel 18. Two trapezoidal protrusions 31 are integrally formed on the flexible sheet 3. The trapezoidal protrusions 31 are made of the same material as the flexible sheet 3. The two trapezoidal protrusions 31 are respectively fitted into the two slots 19. In addition, two receiving cavities 24 are provided on the sample plate 2. The two receiving cavities 24 correspond to the two trapezoidal protrusions 31 respectively, and the two receiving cavities 24 extend to the side of the sample plate 2 adjacent to the flexible sheet 3.

[0029] Meanwhile, two connection ports 23 are also provided on the sample plate 2. The two connection ports 23 correspond to two receiving cavities 24 respectively, and each connection port 23 is connected to the corresponding receiving cavity 24. The connection port 23 is used to connect to an external air pump through a conduit. By drawing air through the external air pump, the part of the flexible sheet 3 corresponding to the receiving cavity 24 is caused to be recessed into the receiving cavity 24, thereby causing the bottom of the trapezoidal protrusion 31 to disengage from the slot 19.

[0030] A magnetic particle-labeled ligand is fixedly disposed inside the reaction chamber 13. The magnetic particles used in the magnetic particle-labeled ligand are composed of iron, cobalt, or nickel compounds, and the magnetic particle-labeled ligand is obtained using existing technology.

[0031] In this embodiment, whole blood samples and diluents are injected through two inlets 21, respectively. The whole blood samples and diluents enter the feed chamber 11, and then flow into the mixing chamber 12 through the feed channel 111 for mixing. The mixing can be promoted by manually shaking the entire chip. Afterward, an external air pump is connected through the connection port 23 corresponding to the trapezoidal protrusion 31 between the reaction chamber 13 and the mixing chamber 12. The external air pump draws air to cause the flexible sheet 3 to deform and cause the bottom of the trapezoidal protrusion 31 to disengage from the corresponding slot 19. At this time, the mixed liquid... The liquid flows into the reaction chamber 13 through the first channel 15, the corresponding slot 19, and the second channel 16 for reaction. After the reaction is completed for a suitable time, an external air pump is connected to another connection port 23 to pump air and cause the bottom of the other trapezoidal protrusion 31 to detach from the other slot 19, thereby allowing the reacted liquid to flow into the light emission detection chamber 14. At the same time, light emission excitation liquid is injected into the light emission detection chamber 14 through the liquid injection port 22. The sample can be analyzed by detecting the light emission signal with an external instrument. Of course, the substrate 1, the sample plate 2, and the flexible sheet 3 are all transparent.

[0032] Example 2 Please see Figures 1-6 Based on Embodiment 1, this embodiment has a fixed base 4 at one end of the substrate 1, a rotating shaft 41 on the fixed base 4, and the two ends of the rotating shaft 41 are movably mounted on the fixed base 4 through bearings. One end of the sample plate 2 is fixedly sleeved on the outside of the rotating shaft 41. By setting the rotating shaft 41, the sample plate 2 can be rotated.

[0033] Meanwhile, two iron sheets 8 are embedded on the substrate 1, and two magnetic blocks 7 are fixed on the sample plate 2. The two magnetic blocks 7 correspond to the two iron sheets 8 respectively. That is, the two magnetic blocks 7 attract the two iron sheets 8 respectively, thereby fixing the sample plate 2 and the flexible sheet 3. When the sample plate 2 is rotated and the attraction force between the magnetic blocks 7 and the iron sheets 8 is overcome, the sample plate 2 and the flexible sheet 3 can be flipped, which makes it easy to clean after use.

[0034] Example 3 See again Figure 1 , Figure 2 and Figure 6 Based on Example 2, this embodiment has a feed tube 5 fixedly installed on each feed port 21, through which whole blood samples and diluents can be easily injected or added; in addition, an injection tube 6 is installed on the injection port 22, through which luminescent excitation liquid can be easily injected or added. Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection, characterized in that, include: The substrate (1) has a mixing chamber (12), a reaction chamber (13) and a light emission detection chamber (14) arranged sequentially on the substrate (1). The substrate (1) also has a feeding chamber (11) and a feeding channel (111) is provided between the feeding chamber (11) and the mixing chamber (12). Sample plate (2) is movably set on substrate (1). Sample plate (2) is provided with inlet (21). Inlet (21) is provided with inlet (11) and inlet (21) is provided with inlet (11). Sample plate (2) is also provided with liquid injection port (22). Liquid injection port (22) corresponds to light emission detection cavity (14). Flexible sheet (3) is located between substrate (1) and template (2), and flexible sheet (3) is disposed on template (2); The substrate (1) is provided with two slots (19), one of which is located between the mixing chamber (12) and the reaction chamber (13). The slot (19) is connected to the mixing chamber (12) by a first channel (15), and the slot (19) is connected to the reaction chamber (13) by a second channel (16). Another slot (19) is located between the reaction chamber (13) and the light emission detection chamber (14), and the other slot (19) is connected to the reaction chamber (13) and the light emission detection chamber (14) through the third channel (17) and the fourth channel (18), respectively.

2. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, It also includes trapezoidal protrusions (31), and two trapezoidal protrusions (31) are provided on the flexible sheet (3), and the two trapezoidal protrusions (31) are respectively arranged in two slots (19).

3. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 2, characterized in that, The template (2) has two receiving cavities (24), and the two receiving cavities (24) correspond to two trapezoidal protrusions (31).

4. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 3, characterized in that, The sample plate (2) has two connection ports (23), which are connected to two receiving cavities (24) respectively. The connection ports (23) are used to connect to an external air pump.

5. The magnetic particle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, The flexible sheet (3) is provided with a first through hole (32), which corresponds to the feed inlet (21). The flexible sheet (3) is also provided with a second through hole (33), which corresponds to the liquid injection port (22).

6. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, A fixing seat (4) is provided at one end of the substrate (1), and a rotating shaft (41) is rotatably provided on the fixing seat (4). One end of the sample plate (2) is fixedly sleeved on the outside of the rotating shaft (41).

7. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, The reaction chamber (13) is equipped with magnetic particle-labeled ligands.

8. The magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, Two iron sheets (8) are provided on the substrate (1), and two magnetic blocks (7) are provided on the template (2). The two magnetic blocks (7) and the two iron sheets (8) are arranged in a one-to-one correspondence.

9. A magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, A feed pipe (5) is provided on the feed inlet (21).

10. A magnetic microparticle chemiluminescence microfluidic chip for whole blood sample detection as described in claim 1, characterized in that, An injection tube (6) is provided on the injection port (22).

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