A multi-process integrated device for obtaining adipose-derived regenerative cell paste

Abstract

A multi-step integrated device for obtaining adipose-derived regenerated cell gel is currently lacking high-precision auxiliary tools to assist in the standardized and refined processing of adipose-derived regenerated cell gel due to the multiple centrifugation operations involved in the extraction process. In this invention, when the main syringe is detachably connected to the top of the opposing support clamp assembly, the inlet and outlet of the push-pull assist syringe are coaxially connected to the inlet and outlet of the main syringe via a first double-ended connector. The main syringe and the push-pull assist syringe, in conjunction with a first pressure sensor, a second pressure sensor, and a drive control assembly, perform a reciprocating cyclic processing of adipose tissue. When the main syringe is detachably connected to the top of the central support clamp processing assembly, the main syringe performs a first-stage layered extraction process via the drive control assembly. When the main syringe is detachably connected to the top of the final support clamp assembly, the main syringe performs a second-stage layered extraction process via the drive control assembly.

Description

Technical Field

[0001] This utility model is specifically a multi-process integrated device for obtaining adipose-derived regenerated cell gel. Background Technology

[0002] The extraction process of adipose-derived regenerative cell gel (adipose stem cell gel, SVF-GEL) is complex primarily due to its multi-step physical processing and precise centrifugation and layering. The multi-step physical processing presents a dual challenge of destruction and purification. During the fat disruption and emulsification process, a fat disruptor is needed to thoroughly break down the mature adipocyte membrane structure, transforming fat particles into an emulsifiable concentrate. This process requires careful control of intensity and time to avoid over-disruption leading to the loss of active ingredients, or under-disruption affecting subsequent purification. The centrifugation layering requires two centrifugations at different speeds. The first centrifugation removes swelling fluid and damaged cells, while the second centrifugation precisely separates the upper oil droplet layer, the middle gel layer, and the lower liquid layer. The middle gel layer comprises only about 10% of the total volume and requires highly precise operation to collect completely, especially during the precise centrifugation and layering process, which demands micron-level accuracy. After the second centrifugation, the SVF-GEL remains in the middle layer, only about 1-2 mm thick. Further purification using a 0.6 mm pore size filter is necessary to remove residual oil droplets and impurities, with a minimum residue level of 5%. Any operational deviation may result in oil droplet contamination or loss of active ingredients. The core components of fatty gum include 1.2-2.1 × 10⁻⁶ per milliliter. 6 Adipose-derived stem cells, collagen, elastin, and various growth factors are involved in the extraction process. Ensuring the activity and concentration of these components during extraction requires extremely precise control over parameters such as temperature and centrifugation speed. Currently, due to the multiple centrifugation operations involved in the extraction process, there are no corresponding high-precision auxiliary tools to standardize the processing of adipose-derived regenerative cell gels in conjunction with a centrifuge. Utility Model Content

[0003] Based on the aforementioned problems, this utility model proposes a multi-step integrated device for obtaining adipose-derived regenerated cell gel. The multi-step integrated device for obtaining adipose-derived regenerated cell gel includes a main syringe, a push-pull assist syringe, a terminal collection syringe, a drive control assembly, a dark chamber, opposing clamping assemblies, a central clamping processing assembly, a terminal clamping assembly, a first double-headed connector, and a sensing system. The opposing clamping assembly, the central clamping processing assembly, the terminal clamping assembly, and the sensing system are arranged vertically side-by-side within the dark chamber. The drive control assembly is connected to the opposing clamping assembly, the central clamping processing assembly, and the terminal clamping assembly. The push-pull assist syringe is located at the bottom of the opposing clamping assembly, and the main syringe is detachably connected to the top of the opposing clamping assembly, the central clamping processing assembly, and the terminal clamping assembly. The sensing system includes a first pressure sensor and a second pressure sensor, both of which are located on the drive control assembly. When the main syringe is detachably connected to the top of the opposing support clamp assembly, the inlet and outlet of the push-pull assist syringe are coaxially connected to the inlet and outlet of the main syringe via a first double-ended connector. The main syringe and the push-pull assist syringe perform a reciprocating cyclic processing of adipose tissue through the cooperation of the first pressure sensor, the second pressure sensor, and the drive control assembly. When the main syringe is detachably connected to the top of the central support clamp processing assembly, the main syringe performs a first-stage layered extraction process through the drive control assembly. When the main syringe is detachably connected to the top of the final support clamp assembly, the main syringe performs a second-stage layered extraction process through the drive control assembly.

[0004] As a preferred embodiment: the drive control assembly includes a first drive component, a second drive component, a third drive component, and a fourth drive component, which are respectively configured to cooperate with the main syringe; the second drive component is configured to cooperate with the push-pull assisted syringe, and the first drive component, the second drive component, the third drive component, and the fourth drive component have the same structure; The first drive assembly includes a first drive motor, a first lead screw, a first lifting plate, a first outer protruding plate, and a plurality of first guide posts. The first drive motor is disposed on the top of the opposing support clamp assembly. The first lead screw and the plurality of first guide posts are vertically arranged side by side on the opposing support clamp assembly. The upper end of the first lead screw is connected to the power output shaft of the first drive motor. The first lifting plate is fitted onto the first lead screw and the plurality of first guide posts. The first lifting plate slides back and forth along the length direction of each first guide post under the drive of the first lead screw. The first outer protruding plate is fixedly connected to the first lifting plate. A first limiting part that cooperates with the piston rod in the main syringe is provided on the bottom surface of the first outer protruding plate. The first pressure sensor is disposed in the first limiting part. The second drive assembly is configured in cooperation with the second pressure sensor.

[0005] As a preferred embodiment: an inner support frame is provided between the opposing support clamp assembly, the middle support clamp processing assembly, and the end support clamp assembly. The inner support frame includes two horizontal supports and three longitudinal support columns. The inner support frame is abutted against the inner wall of the darkroom. The two horizontal supports are horizontally arranged from top to bottom on the inner wall of the darkroom, and the three longitudinal support columns are vertically arranged side by side between the two horizontal supports.

[0006] As a preferred embodiment: the opposing support clamp assembly includes an upper support plate, a middle support plate, a lower support plate, a first clamping member, a second clamping member, and a first positioning clamp. The upper support plate and the middle support plate are arranged horizontally side by side from top to bottom on two transverse supports. The lower support plate is located at the bottom of the dark box. The second drive assembly is located between the middle support plate and the lower support plate. The first drive assembly is located between the upper support plate and the middle support plate. A longitudinal support column is provided between the upper support plate and the middle support plate. The first clamping member and the second clamping member are located on the longitudinal support column between the upper support plate and the middle support plate. The first clamping member and the second clamping member are detachably connected to the main syringe and the push-pull assist syringe, respectively. The first positioning clamp is located on the upper support plate. One end of the first positioning clamp is elastically hinged to the upper support plate, and the other end of the first positioning clamp presses the main syringe onto the upper support plate.

[0007] As a preferred embodiment: the central support clamping assembly includes a first support plate, a third clamping member, a second positioning clamp, and a storage and processing container. The first support plate is horizontally connected to the transverse support, and one end of the first support plate is on the same horizontal plane as its adjacent upper support plate. The second positioning clamp is disposed on the first support plate and is detachably connected to the main syringe. The third clamping member and the storage and processing container are disposed sequentially from top to bottom on the second longitudinal support column among the three longitudinal support columns. The third clamping member is detachably connected to the main syringe, and the inlet and outlet of the main syringe are oriented towards the storage and processing container.

[0008] As a preferred embodiment: the end-positioned support clamp assembly includes a second support plate, a fourth clamping member, a third positioning clamp, and a fifth clamping clamp. The second support plate is horizontally connected to the transverse support, and one end of the second support plate is on the same horizontal plane as the adjacent first support plate. The third positioning clamp is disposed on the second support plate and is detachably connected to the main syringe. The fourth clamping member and the fifth clamping clamp are disposed sequentially from top to bottom on the third longitudinal support column among the three longitudinal support columns. The fourth clamping member is detachably connected to the main syringe, and the fifth clamping clamp is detachably connected to the end-collecting syringe. The inlet and outlet of the main syringe and the inlet and outlet of the end-collecting syringe are coaxially connected.

[0009] As a preferred embodiment: a first recognition camera and a second recognition camera are fitted together on the middle support clamping assembly and the end support clamping assembly. The first recognition camera is slidably connected to the longitudinal support column nearby, and the lens of the first recognition camera is set towards the main syringe. The second recognition camera is slidably connected to the longitudinal support column nearby, and the lens of the second recognition camera is set towards the main syringe.

[0010] As a preferred option, it also includes multiple disinfection tubes arranged from top to bottom inside the darkroom. A door is provided on one side of the darkroom, and an adapter layer for the darkroom is provided on the inner wall of the door.

[0011] As a preferred embodiment: the sensing system further includes a main controller, a first photosensitive signal emitting sensor and a first photosensitive signal receiving sensor. The first photosensitive signal emitting sensor is provided on the side of the first lifting plate facing the dark box. A second photosensitive signal emitting sensor that cooperates with the first lifting plate is provided on the dark box. The first photosensitive signal emitting sensor, the first photosensitive signal receiving sensor, the first pressure sensor and the second pressure sensor are respectively electrically connected to the main controller.

[0012] Compared with the prior art, the present invention has the following beneficial effects: This invention relates to a specialized device for the precise extraction of adipose-derived regenerated cell gel using multiple alternating centrifugation operations. The device comprises a main syringe, a push-pull assist syringe, a terminal collection syringe, a drive control assembly, a dark chamber, opposing clamping assemblies, a central clamping processing assembly, and a terminal clamping assembly. These components work together to sequentially process the stock solution through adipose tissue reciprocating circulation, a single centrifugation, a single layered extraction, a second centrifugation, and a second layered extraction, ultimately forming a refined adipose-derived regenerated cell gel. This allows for precise, on-demand processing of the adipose-derived regenerated cell gel, facilitating standardized extraction and enabling the entire process to be completed without changing tools, with the main syringe serving as the sole contact point for the adipose-derived regenerated cell gel. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the rear view structure of this utility model; Figure 2 This is a schematic diagram of the main structure of this utility model; Figure 3 This is a first three-dimensional structural diagram of the present invention; Figure 4 This is a second three-dimensional structural diagram of the present invention. Only the back plate of the dark box is shown in the figure, and other parts of the dark box are omitted. Figure 5 This is a schematic diagram of the third three-dimensional structure of the present invention; Figure 6 This is a schematic diagram of the fourth three-dimensional structure of the present invention; Figure 7 This is a schematic diagram of the fifth three-dimensional structure of the present utility model; Figure 8 A three-dimensional structural diagram of the structure corresponding to the repeated cyclic processing of adipose tissue; Figure 9 This is a three-dimensional structural diagram of the structure corresponding to a single layer extraction process. Figure 10 This is a three-dimensional structural diagram of the structure corresponding to the secondary layer extraction process.

[0014] In the diagram, 1-main syringe; 2-push-pull assist syringe; 3-end collection syringe; 4-drive control assembly; 4-1-first drive motor; 4-2-first lead screw; 4-3-first lifting plate; 4-4-first outward protruding plate; 4-5-first guide post; 4-6-first limiting part; 5-dark box; 6-opposing support clamp assembly; 6-1-upper support plate; 6-2-middle support plate; 6-3-lower support plate; 6-4-first clamping member; 6-5-second clamping member; 6-6-first positioning clamp; 7-middle support clamp processing assembly; 7-1-first support plate; 7-2-third clamping member; 7-3-second positioning clamp; 7-4-storage processing container; 8-end support clamp assembly; 8-1-second support plate; 8-2-Fourth clamping component; 8-3-Third positioning clamp; 8-4-Fifth clamping clamp; 9-Inner support frame; 9-1-Horizontal bracket; 9-2-Longitudinal support column; 10-First recognition camera; 11-First double-headed connector; 12-Pressure sensor; 13-Disinfection tube; 14-Fan door; 15-Adaptor layer for darkroom; 16-Support plate for motor; 17-Second recognition camera; 18-Second double-headed connector; 19-First limit switch; 20-Second limit switch; 21-First push head; 22-Third limit switch; 23-Second push head; 24-First photosensitive receiving signal sensor; 40-First drive assembly; 41-Second drive assembly; 42-Third drive assembly; 43-Fourth drive assembly. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Specific implementation method one: Combining Figures 1 to 10 This embodiment describes a multi-step integrated device for obtaining adipose-derived regenerated cell gel, comprising a main syringe 1, a push-pull assist syringe 2, a terminal collection syringe 3, a drive control assembly 4, a dark chamber 5, opposing clamping assemblies 6, a central clamping processing assembly 7, a terminal clamping assembly 8, a first double-headed connector 11, and a sensing system. The opposing clamping assembly 6, the central clamping processing assembly 7, the terminal clamping assembly 8, and the sensing system are arranged vertically side-by-side within the dark chamber 5. The drive control assembly 4 is connected to the opposing clamping assembly 6, the central clamping processing assembly 7, and the terminal clamping assembly 8. The push-pull assist syringe 2 is located at the bottom of the opposing clamping assembly 6, and the main syringe 1 is detachably connected to the top of the opposing clamping assembly 6, the central clamping processing assembly 7, and the terminal clamping assembly 8. The sensing system includes a first pressure sensor 12 and a second pressure sensor, both of which are located on the drive control assembly 4. When the main injector 1 is detachably connected to the top of the opposing support clamp assembly 6, the inlet and outlet of the push-pull assist injector 2 are coaxially connected to the inlet and outlet of the main injector 1 through the first double-headed connector 11. The main injector 1 and the push-pull assist injector 2 perform a reciprocating cyclic processing of adipose tissue through the cooperation of the first pressure sensor 12, the second pressure sensor, and the drive control assembly 4. When the main injector 1 is detachably connected to the top of the central support clamp processing assembly 7, the main injector 1 performs a first-stage layered extraction process through the drive control assembly 4. When the main injector 1 is detachably connected to the top of the final support clamp assembly 8, the main injector 1 performs a second-stage layered extraction process through the drive control assembly 4.

[0017] The main syringe 1 of this invention contains adipose tissue stock solution, which is the source of adipose-derived regenerated cell gel extraction. The main syringe 1 and the push-pull assist syringe 2 cooperate with the drive control assembly 4 and the opposing support clamp assembly 6 to complete the first treatment of the stock solution, forming a first-processed liquid. The main syringe 1, in cooperation with the drive control assembly 4 and the central support clamp processing assembly 7, completes the second treatment of the first-processed liquid to form a second-processed liquid. The main syringe 1, in cooperation with the drive control assembly 4 and the final support clamp assembly 8, completes the third treatment of the second-processed liquid to form a third-processed liquid. The third-processed liquid is the extracted adipose-derived regenerated cell gel.

[0018] Specific Implementation Method Two: This implementation method is a further limitation of Specific Implementation Method One. In this implementation method, the drive control assembly 4 includes a first drive component 40, a second drive component 41, a third drive component 42, and a fourth drive component 43. The first drive component 40, the third drive component 42, and the fourth drive component 43 are respectively configured to cooperate with the main injector 1; the second drive component 41 is configured to cooperate with the push-pull assist injector 2. The first drive component 40, the second drive component 41, the third drive component 42, and the fourth drive component 43 have the same structure. The first drive assembly 40 includes a first drive motor 4-1, a first lead screw 4-2, a first lifting plate 4-3, a first external convex plate 4-4, and a plurality of first guide posts 4-5. The first drive motor 4-1 is disposed on the top of the opposing support clamp assembly 6. The first lead screw 4-2 and the plurality of first guide posts 4-5 are vertically arranged side by side on the opposing support clamp assembly 6. The upper end of the first lead screw 4-2 is connected to the power output shaft of the first drive motor 4-1. The first lifting plate 4-3 is fitted onto the first lead screw 4-2 and the plurality of first guide posts 4-5. The first lifting plate 4-3 slides back and forth along the length direction of each first guide post 4-5 under the drive of the first lead screw 4-2. The first external convex plate 4-4 is fixedly connected to the first lifting plate 4-3. A first limiting part 4-6 is provided on the bottom surface of the first external convex plate 4-4 to cooperate with the piston rod in the main syringe 1. The first pressure sensor 12 is disposed in the first limiting part 4-6. The second drive assembly 41 is configured to cooperate with the second pressure sensor.

[0019] In this embodiment, the first drive motor 4-1 is positioned and fitted with a motor support plate 16, which is a horizontally arranged plate. The plate structure is used to fix the position of each drive motor in the first drive assembly 40, the third drive assembly 42 and the fourth drive assembly 43.

[0020] The sensing system described in this embodiment also includes a main controller, a first photosensitive signal emitting sensor and a first photosensitive signal receiving sensor 24. The first photosensitive signal emitting sensor is provided on the side of the first lifting plate 4-3 facing the dark box 5. The dark box 5 is provided with a second photosensitive signal emitting sensor that cooperates with the first lifting plate 4-3. The first photosensitive signal emitting sensor, the first photosensitive signal receiving sensor 24, the first pressure sensor 12 and the second pressure sensor are electrically connected to the main controller.

[0021] The working principle of the first photosensitive signal emitting sensor and the first photosensitive signal receiving sensor 24 in the sensing system is as follows: the position of the first photosensitive signal emitting sensor indicates the moving position of the first lifting plate 4-3. When the first photosensitive signal emitting sensor moves to the opposite side of the first photosensitive signal receiving sensor 24, the first photosensitive signal emitting sensor sends a signal to the first photosensitive signal receiving sensor 24. The first photosensitive signal receiving sensor 24 transmits the signal to the main controller, indicating that the first lifting plate 4-3 has reached the predetermined limit position. The main controller then controls the first drive motor 4-1 to stop running.

[0022] The working principle of the first pressure sensor 12 and the second pressure sensor in the sensing system is as follows: the first lifting plate 4-3 moves down to the main syringe 1 and presses down the piston rod of the main syringe 1 with a certain pressure. When the pressure threshold is reached, the first pressure sensor 12 transmits a signal to the main controller, and the main controller controls the first drive motor 4-1 to stop running.

[0023] Specific Implementation Method 3: This implementation method is a further limitation of Specific Implementation Method 1 or 2. In this implementation method, an inner support frame 9 is provided between the opposing support clamp assembly 6, the central support clamp processing assembly 7, and the final support clamp assembly 8. The inner support frame 9 includes two horizontal supports 9-1 and three longitudinal support columns 9-2. The inner support frame 9 is abutted against the inner wall of the dark box 5. The two horizontal supports 9-1 are arranged horizontally from top to bottom on the inner wall of the dark box 5. The three longitudinal support columns 9-2 are arranged vertically side by side between the two horizontal supports 9-1. The opposing support clamp assembly 6 includes an upper support plate 6-1, a central support plate 6-2, a lower support plate 6-3, a first clamping member 6-4, a second clamping member 6-5, and a first positioning clamp 6-6. The upper support plate 6-1 and the central support plate 6-2 are arranged horizontally side by side from top to bottom on the two horizontal supports 9-1. -1 On the bottom of the dark box 5, the lower support plate 6-3 is set on the bottom of the dark box 5. The second drive assembly 41 is set between the middle support plate 6-2 and the lower support plate 6-3. The first drive assembly 40 is set between the upper support plate 6-1 and the middle support plate 6-2. A longitudinal support column 9-2 is set between the upper support plate 6-1 and the middle support plate 6-2. The first clamping member 6-4 and the second clamping member 6-5 are set on the longitudinal support column 9-2 between the upper support plate 6-1 and the middle support plate 6-2. The first clamping member 6-4 and the second clamping member 6-5 are detachably connected to the main syringe 1 and the push-pull assist syringe 2, respectively. The first positioning clamp 6-6 is set on the upper support plate 6-1. One end of the first positioning clamp 6-6 is elastically hinged to the upper support plate 6-1. The other end of the first positioning clamp 6-6 presses the main syringe 1 on the upper support plate 6-1. The central support clamping assembly 7 includes a first support plate 7-1, a third clamping member 7-2, a second positioning clamp 7-3, and a storage and processing container 7-4. The first support plate 7-1 is horizontally connected to the transverse support 9-1. One end of the first support plate 7-1 is on the same horizontal plane as its adjacent upper support plate 6-1. The second positioning clamp 7-3 is disposed on the first support plate 7-1 and is detachably connected to the main syringe 1. The third clamping member 7-2 and the storage and processing container 7-4 are disposed sequentially from top to bottom on the second longitudinal support column 9-2 among the three longitudinal support columns 9-2. The third clamping member 7-2 is detachably connected to the main syringe 1, and the inlet and outlet of the main syringe 1 are oriented towards the storage and processing container 7-4. The end-positioned support clamp assembly 8 includes a second support plate 8-1, a fourth clamping member 8-2, a third positioning clamp 8-3, and a fifth clamping clamp 8-4. The second support plate 8-1 is horizontally connected to the transverse support 9-1. One end of the second support plate 8-1 is on the same horizontal plane as the adjacent first support plate 7-1. The third positioning clamp 8-3 is disposed on the second support plate 8-1 and is detachably connected to the main syringe 1. The fourth clamping member 8-2 and the fifth clamping clamp 8-4 are disposed sequentially from top to bottom on the third longitudinal support column 9-2 among the three longitudinal support columns 9-2. The fourth clamping member 8-2 is detachably connected to the main syringe 1, and the fifth clamping clamp 8-4 is detachably connected to the end-collecting syringe 3. The inlet and outlet of the main syringe 1 and the inlet and outlet of the end-collecting syringe 3 are coaxially connected.

[0024] Specific Implementation Method Four: This implementation method is a further limitation of Specific Implementation Method One, Two or Three. In this implementation method, the inlet and outlet of the push-pull assist syringe 2 are coaxially connected to the inlet and outlet of the main syringe 1 through the first double-headed connector 11.

[0025] Specific Implementation Method 5: This implementation method is a further limitation of Specific Implementation Methods 1, 2, 3 or 4. In this implementation method, the middle support clamp processing component 7 and the end support clamp component 8 are equipped with a first identification camera 10 and a second identification camera 17. The first identification camera 10 is slidably connected to the longitudinal support column 9-2 nearby, and the lens of the first identification camera 10 is set towards the main injector 1. The second identification camera 17 is slidably connected to the longitudinal support column 9-2 nearby, and the lens of the second identification camera 17 is set towards the main injector 1.

[0026] Specific implementation method six: This implementation method is a further limitation of specific implementation methods one, two, three, four or five. A pressure sensor 12 is provided on the bottom surface of the first outer convex plate 4-4.

[0027] Specific Implementation Method Seven: This implementation method is a further limitation of Specific Implementation Method One, Two, Three, Four, Five or Six, and also includes multiple disinfection tubes 13, which are arranged from top to bottom inside the dark box 5.

[0028] Specific implementation method eight: This implementation method is a further limitation of specific implementation methods one, two, three, four, five, six or seven. A fan door 14 is provided on one side of the dark box 5, and an adapter layer 15 for the dark room is provided on the inner wall of the fan door 14.

[0029] This device is equipped with a control system, which is an existing system, used to control each drive motor and each pressure sensor 12 in this device.

[0030] Specific Implementation Method Nine: This implementation method is a further limitation of Specific Implementation Methods One, Two, Three, Four, Five, Six, Seven, or Eight. In this implementation method, the support frame 9 is provided with a first limit switch 19, a second limit switch 20, a third limit switch 22, and a fourth limit switch that cooperate with the first drive component 40, the second drive component 41, the third drive component 42, and the fourth drive component 43 in the drive control assembly 4. The first limit switch 19, the second limit switch 20, the third limit switch 22, and the fourth limit switch are all photosensitive sensors. The first limit switch 10, the second limit switch 20, the third limit switch 22, and the fourth limit switch are respectively located on the rear side of the vertical position of each processing step of the main injector 1. The photosensitive sensors realize the quantitative acquisition of the path height of each lifting plate through the obstruction of each lifting plate.

[0031] Specific Implementation Method 10: This implementation method is a further limitation of Specific Implementation Methods 1, 2, 3, 4, 5, 6, 7, 8 or 9. In this implementation method, after centrifugation, the piston rod of the main syringe 1 can be replaced with a structure of a first push head 21 and a second push head 23 with a relatively shorter length. When the first push head 21 or the second push head 23 is used, the lifting plates of the corresponding third drive assembly 42 and the fourth drive assembly 43 are respectively provided with connectors that engage with the top of the first push head 21 or the second push head 23. The connectors can be cross-shaped snap-fit ​​heads or other detachable connection structures.

[0032] The specific operation process of this utility model is as follows: First, the initial position calibration process of the first drive component 40 is performed. The first drive component 40 is started. The first drive motor 4-1 drives the first lifting plate 4-3 to move up and down through the first lead screw 4-2 and simultaneously pushes the piston rod of the main syringe 1 to move. When the pressure sensor 12 on the first limit part detects that the pressure value exceeds the threshold, the sensor sends a signal to the control system. The control system controls the first drive motor 4-1 to stop rotating. At this time, the horizontal position of the first lifting plate 4-3 is the sample loading positioning position. The stock solution containing adipose tissue is injected into the main syringe 1. The first double-ended connector 11 is connected to the inlet and outlet of the main syringe 1. The main syringe 1 with the first double-ended connector 11 is placed into the first clamping member 6-4. The push-pull assist syringe 2 is placed into the second clamping member 6-5 and fixed by the first positioning clamp 6-6. The first drive assembly 40 and the second drive assembly 41 are activated. The piston rod of the main syringe 1 is pushed by the first lifting plate 4-3 in the first drive assembly 40, reducing the internal empty stroke of the main syringe 1 until the pressure sensor 12 on the bottom surface of the first outer protrusion plate 4-4 reaches the predetermined pressure value and then the pushing stops. At this time, the position of the first lifting plate 4-3 is the injection start position, thereby completing the emptying preparation process of the main syringe 1. At the same time, and in the same way as the above operation, after the push-pull assist syringe 2 completes the emptying preparation process under the control of the second drive assembly 41, the inlet and outlet of the main syringe 1 are connected to the inlet and outlet of the push-pull assist syringe 2 through the first double-headed connector 11. According to preset parameters, the drive control assembly controls the first drive motor 4-1 to rotate at a set speed. The first lead screw 4-2 drives the first lifting plate 4-3 to push the main syringe 1 at a predetermined speed, squeezing the original liquid containing adipose tissue from the main syringe 1 into the push-pull assist syringe 2. After the main syringe 1 reaches its maximum stroke, the first drive motor 4-1 reverses, and the first lifting plate 4-3 moves in the opposite direction. The movement of the first lifting plate 4-3 synchronously pushes the push rod of the push-pull assist syringe 2, pushing the original liquid containing adipose tissue back into the main syringe 1, completing one push-pull cycle. The push-pull cycle is performed a predetermined number of times according to predetermined requirements, thereby completing the reciprocating cycle processing of adipose tissue. After the reciprocating cycle processing of adipose tissue, the original liquid containing adipose tissue in the main syringe 1 is in an emulsified state.

[0033] After the above-mentioned fat emulsification and injection process is completed, the first drive component 40 and the second drive component 41 are reset respectively. After the operator checks the emulsification completion status and the display reaches the predetermined requirements, the main syringe 1 is removed, and the emulsified fat tissue in the main syringe 1 is transferred to a centrifuge for centrifugation. The above-mentioned centrifugation process is the same as the working principle of existing centrifugation processes.

[0034] After centrifugation, the main syringe 1 completes a layered extraction process through the cooperation of the central support clamping assembly 7 and the third drive assembly 42 in the drive control assembly 4. After the main syringe 1 is clamped and positioned by the third clamping member 7-2 and the second positioning clamp 7-3, the first recognition camera 10 is activated to take pictures of the liquid remaining in the main syringe 1. The image of the first recognition camera 10 is analyzed and processed by the image recognition algorithm to accurately identify the layered interface between blood and fat-derived regenerated cell glue, calculate the total volume of blood in the main syringe 1, calculate and obtain the descent distance of the piston rod in the main syringe 1 in the third drive assembly 42 based on the blood content data, and activate the third drive assembly 42 after initial position calibration to drive the piston rod of the main syringe 1 to move down. When the pressure sensor on the third drive assembly 42 detects that the pressure exceeds the threshold, it sends a signal to the control system to stop the third drive assembly 42 from rotating. The blood discharged from the inlet and outlet of the main syringe 1 enters the collection and processing container 7-4, completing a layered extraction process of blood discharge. After the formation of adipose-derived regenerated cell gel and refined blood-water separation is completed, the operator checks the completion status of blood-water separation through the human-machine interface again. If the completion status meets the predetermined requirements, the third drive component 42 is reset, the main syringe 1 that has completed blood-water separation is removed and transferred into the centrifuge for secondary centrifugation. The above centrifugation process is the same as the working principle of the existing centrifugation process.

[0035] The fourth driving component 43 undergoes an initial position calibration process, which is similar to the initial position calibration process of the first driving component 40. After initial position calibration, the fourth driving component 43, in conjunction with the main syringe 1 and the terminal collection syringe 3, completes the secondary layered extraction process. After the operator centrifuges the main syringe 1 twice and drains the residual blood again, they coaxially connect it to the end collection syringe 3 via the second double-ended connector 18. The main syringe 1 and the end collection syringe 3 are then installed on the end support clamp assembly 8. The fan door 15 is closed to ensure the dark chamber 5 is in a closed, light-proof state. The second recognition camera 17 is activated to capture real-time images of the material inside the main syringe 1. The image recognition algorithm analyzes and processes the images captured by the second recognition camera 17 to accurately identify the layering interface between the adipose-derived regenerated cell glue and the upper oil layer in the main syringe 1, and calculates the adipose-derived regenerated cell glue within the main syringe 1. The total volume of the regenerated cell gel is determined. The control system issues a command, and the fourth drive component 43 drives the main syringe 1 to move downward. The control system calculates the predetermined descent distance of the main syringe 1 based on the content data of the fat-derived regenerated cell gel and issues a control command to the fourth drive component 43. The fourth drive component 43 pushes the material in the main syringe 1 through the second double-headed connector 18 into the end collection syringe 3. The substance in the end collection syringe 3 is the obtained fat-derived regenerated cell gel. At this time, the transfer of the fat-derived regenerated cell gel is completed, and the upper oil is retained in the main syringe 1. At this time, the upper oil is waste. End of reset: After the transfer of adipose-derived regenerated cell gel is completed, the operator can check the transfer completion signal through the human-machine interface. Once the signal reaches the predetermined requirement, the control system will issue a command to control the fourth drive component 43 to reset, and the end collection syringe 3 containing the adipose-derived regenerated cell gel can be removed.

[0036] This embodiment also includes a disinfection process where the used dark box 5 is replenished through multiple disinfection tubes 13. Specifically, after closing the fan door 14, multiple disinfection tubes 13 are activated for disinfection. The disinfection time and intensity are determined according to specific requirements. The method in this embodiment can only be implemented using this acquisition device. Structures and connections not mentioned in this acquisition device are the same as in specific embodiments one, two, three, four, five, six, seven, eight, nine, or ten.

Claims

1. A multi-process integrated device for obtaining adipose-derived regenerative cell gel, characterized by: The device includes a main syringe (1), a push-pull assist syringe (2), an end collection syringe (3), a drive control assembly (4), a dark box (5), an opposing support clamp assembly (6), a central support clamp processing assembly (7), an end support clamp assembly (8), a first double-headed connector (11), and a sensing system. The opposing support clamp assembly (6), the central support clamp processing assembly (7), the end support clamp assembly (8), and the sensing system are arranged vertically side by side in the dark box (5). The drive control assembly (4) is connected to the opposing support clamp assembly (6), the central support clamp processing assembly (7), and the end support clamp assembly (8) respectively. The push-pull assist syringe (2) is located at the bottom of the opposing support clamp assembly (6). The main syringe (1) is detachably connected to the top of the opposing support clamp assembly (6), the central support clamp processing assembly (7), and the end support clamp assembly (8) respectively. The sensing system includes a first pressure sensor (12) and a second pressure sensor. Both the first pressure sensor (12) and the second pressure sensor are on the drive control assembly (4). When the main syringe (1) is detachably connected to the top of the opposing support clamp assembly (6), the inlet and outlet of the push-pull assist syringe (2) are coaxially connected to the inlet and outlet of the main syringe (1) through the first double-headed connector (11). The main syringe (1) and the push-pull assist syringe (2) perform a reciprocating cyclic processing of adipose tissue through the cooperation of the first pressure sensor (12), the second pressure sensor and the drive control assembly (4). When the main syringe (1) is detachably connected to the top of the central support clamp processing assembly (7), the main syringe (1) performs a first layered extraction process through the drive control assembly (4). When the main syringe (1) is detachably connected to the top of the final support clamp assembly (8), the main syringe (1) performs a second layered extraction process through the drive control assembly (4).

2. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 1, wherein: The drive control assembly (4) includes a first drive component (40), a second drive component (41), a third drive component (42), and a fourth drive component (43). The first drive component (40), the third drive component (42), and the fourth drive component (43) are respectively configured to cooperate with the main syringe (1); the second drive component (41) is configured to cooperate with the push-pull assist syringe (2). The first drive component (40), the second drive component (41), the third drive component (42), and the fourth drive component (43) have the same structure. The first drive assembly (40) includes a first drive motor (4-1), a first lead screw (4-2), a first lifting plate (4-3), a first external convex plate (4-4), and a plurality of first guide posts (4-5). The first drive motor (4-1) is mounted on the top of the opposing support clamp assembly (6). The first lead screw (4-2) and the plurality of first guide posts (4-5) are vertically arranged side by side on the opposing support clamp assembly (6). The upper end of the first lead screw (4-2) is connected to the power output shaft of the first drive motor (4-1). The first lifting plate (4-3) is fitted onto the first lead screw. On the lever (4-2) and multiple first guide posts (4-5), the first lifting plate (4-3) slides back and forth along the length direction of each first guide post (4-5) under the drive of the first lead screw (4-2). The first outer protruding plate (4-4) is fixedly connected to the first lifting plate (4-3). The bottom surface of the first outer protruding plate (4-4) is provided with a first limiting part (4-6) that cooperates with the piston rod in the main syringe (1). The first pressure sensor (12) is provided in the first limiting part (4-6). The second driving assembly (41) is configured in cooperation with the second pressure sensor.

3. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 1, wherein: The sensing system also includes a main controller, a first photosensitive signal emitting sensor and a first photosensitive signal receiving sensor (24). The first photosensitive signal emitting sensor is provided on the side of the first lifting plate (4-3) facing the dark box (5). The dark box (5) is provided with a second photosensitive signal emitting sensor that cooperates with the first lifting plate (4-3). The first photosensitive signal emitting sensor, the first photosensitive signal receiving sensor (24), the first pressure sensor (12) and the second pressure sensor are electrically connected to the main controller.

4. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 3, wherein: An inner support frame (9) is provided between the opposing support clamp assembly (6), the middle support clamp processing assembly (7), and the end support clamp assembly (8). The inner support frame (9) includes two horizontal supports (9-1) and three longitudinal support columns (9-2). The inner support frame (9) is abutted against the inner wall of the dark box (5). The two horizontal supports (9-1) are horizontally arranged from top to bottom on the inner wall of the dark box (5). The three longitudinal support columns (9-2) are vertically arranged side by side between the two horizontal supports (9-1).

5. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 4, wherein: The opposing support clamp assembly (6) includes an upper support plate (6-1), a middle support plate (6-2), a lower support plate (6-3), a first clamping member (6-4), a second clamping member (6-5), and a first positioning clamp (6-6). The upper support plate (6-1) and the middle support plate (6-2) are arranged horizontally side by side from top to bottom on two transverse supports (9-1). The lower support plate (6-3) is located on the bottom of the dark box (5). The second drive assembly (41) is located between the middle support plate (6-2) and the lower support plate (6-3). The first drive assembly (40) is located between the upper support plate (6-1) and the middle support plate (6-2). The upper support plate (6-1) is positioned between the middle support plate (6-2) and the lower support plate (6-3). 1) A longitudinal support column (9-2) is provided between the upper support plate (6-1) and the central support plate (6-2). The first clamping member (6-4) and the second clamping member (6-5) are provided on the longitudinal support column (9-2) between the upper support plate (6-1) and the central support plate (6-2). The first clamping member (6-4) and the second clamping member (6-5) are detachably connected to the main syringe (1) and the push-pull assist syringe (2) respectively. The first positioning clamp (6-6) is provided on the upper support plate (6-1). One end of the first positioning clamp (6-6) is elastically hinged to the upper support plate (6-1), and the other end of the first positioning clamp (6-6) presses the main syringe (1) onto the upper support plate (6-1).

6. The multi-process integrated apparatus for obtaining adipose-derived regenerated cell gel according to claim 3, 4 or 5, characterized in that: The central support clamp processing assembly (7) includes a first support plate (7-1), a third clamping member (7-2), a second positioning clamp (7-3), and a storage processing container (7-4). The first support plate (7-1) is horizontally connected to the transverse support (9-1). One end of the first support plate (7-1) is on the same horizontal plane as its adjacent upper support plate (6-1). The second positioning clamp (7-3) is set on the first support plate (7-1) and is detachably connected to the main syringe (1). The third clamping member (7-2) and the storage processing container (7-4) are set from top to bottom on the second longitudinal support column (9-2) of the three longitudinal support columns (9-2). The third clamping member (7-2) is detachably connected to the main syringe (1). The inlet and outlet of the main syringe (1) are set towards the storage processing container (7-4).

7. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 6, wherein: The end-positioned support clamp assembly (8) includes a second support plate (8-1), a fourth clamping member (8-2), a third positioning clamp (8-3), and a fifth clamping clamp (8-4). The second support plate (8-1) is horizontally connected to the transverse support (9-1). One end of the second support plate (8-1) is on the same horizontal plane as the adjacent first support plate (7-1). The third positioning clamp (8-3) is set on the second support plate (8-1) and is detachably connected to the main syringe (1). The fourth clamping member (8-2) and the fifth clamping clamp (8-4) are set from top to bottom on the third longitudinal support column (9-2) of the three longitudinal support columns (9-2). The fourth clamping member (8-2) is detachably connected to the main syringe (1), and the fifth clamping clamp (8-4) is detachably connected to the end-collecting syringe (3). The inlet and outlet of the main syringe (1) are coaxially connected to the inlet and outlet of the end-collecting syringe (3).

8. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 1, wherein: A first identification camera (10) and a second identification camera (17) are provided on the middle support clamp processing component (7) and the end support clamp component (8). The first identification camera (10) is slidably connected to the longitudinal support column (9-2) near it, and the lens of the first identification camera (10) is set towards the main syringe (1). The second identification camera (17) is slidably connected to the longitudinal support column (9-2) near it, and the lens of the second identification camera (17) is set towards the main syringe (1).

9. The multi-process integrated device for obtaining adipose-derived regenerative cell paste according to claim 1, wherein: It also includes multiple disinfection tubes (13), which are arranged from top to bottom inside the dark box (5). A door (14) is provided on one side of the dark box (5), and an adapter layer (15) for the dark room is provided on the inner wall of the door (14).

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