A cell dispensing apparatus

Abstract

The utility model relates to cell culture equipment technical field, concretely is a kind of cell subpackaging equipment, including box, mix uniform area, subpackaging area, liquid path control module, refrigeration system and air duct;The mix uniform area is located in left upper portion in box and is equipped with mix uniform subassembly, the liquid path control module is located in left lower portion in box, the subpackaging area is located in right side in box and is equipped with at least one group subpackaging subassembly, the box area where the mix uniform area is located is equipped with several air inlets to make the cold air of refrigeration system enter, one end of air duct is communicated with one of the air inlets, another end is located in alignment subpackaging area upper portion and is equipped with several air outlets.The subpackaging equipment makes mix uniform area and subpackaging area can realize low-temperature environment, is favorable to guarantee cell sustained good state when subpackaging.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment technology, specifically a cell dispensing device. Background Technology

[0002] Cell dispensing equipment is an automated or semi-automated device specifically designed for the accurate and rapid dispensing of cell samples, culture media, or other relevant solutions according to preset quantities and conditions in biological laboratories. This type of equipment typically features high precision, high efficiency, and aseptic operation to meet the accuracy and safety requirements of cell experiments.

[0003] In cell processing, cells need to be centrifuged, mixed with cryopreservation solution, and aliquoted for subsequent use. Cell aliquoting equipment requires low-temperature storage of cells during aliquoting to minimize toxicity, typically maintained at 15-25°C. Temperature-sensitive cells such as stem cells and nerve cells require even lower temperatures, such as 0-4°C. Traditional cell aliquoting equipment only cools the mixing zone, neglecting the low-temperature requirements of the aliquoting zone. Since numerous aliquot bags are placed in the aliquoting zone during aliquoting, it is crucial to maintain a temperature as consistent as possible with the mixing zone to ensure stable cell metabolic rates and prevent cell stress caused by temperature fluctuations. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a cell dispensing device with a rational structure and an integrated design of the mixing and dispensing zones, enabling both regions to achieve the low-temperature environment required for cell dispensing. The technical solution adopted by this invention is as follows: A cell dispensing device includes a housing, a mixing zone, a dispensing zone, a liquid path control module, a refrigeration system, and an air duct. The mixing zone is located on the upper left side of the housing and is equipped with a mixing component. The liquid path control module is located on the lower left side of the housing. The dispensing zone is located on the right side of the housing and is equipped with at least one dispensing component. The housing area where the mixing zone is located has several air inlets to allow cold air from the refrigeration system to enter. One end of the air duct is connected to one of the air inlets, and the other end is located above the dispensing zone and is equipped with several air outlets.

[0005] The cell dispensing equipment described above includes a clamping tube module arranged vertically and a dispensing rack for placing dispensing bags. The dispensing rack includes a bottom plate, a back plate, and several spaced vertical plates. The bottom plate and the vertical plates are provided with several through holes on their surfaces.

[0006] The cell dispensing equipment described above is equipped with a temperature sensor or thermometer in the dispensing area.

[0007] The beneficial effects of this invention are: this dispensing equipment enables both the mixing zone and the dispensing zone to achieve a low-temperature environment, which helps to ensure that the cells remain in good condition during dispensing. Attached Figure Description

[0008] Figure 1 and Figure 2 This is a schematic diagram of the structure of an embodiment of the present utility model; Figure 3 This is a schematic diagram of the air duct installation according to an embodiment of the present utility model; Figure 4 This is a schematic diagram of the inner shell of the box body according to an embodiment of the present utility model (the external profile frame is not shown). Figure 5 This is a schematic diagram of the packaging rack according to an embodiment of the present utility model; Figure 6 This is a schematic diagram of the refrigeration principle of an embodiment of the present invention. In the diagram: 1 is the mixing zone, 2 is the liquid circuit control module, 3 is the clamping tube module, 4 is the dispensing rack, 5 is the evaporator assembly, 6 is the air duct, 7 is the temperature sensor, 41 is the base plate, 42 is the vertical plate, 43 is the rear plate, 51 is the upper air outlet, 52 is the lower air outlet, and 61 is the air outlet. Detailed Implementation

[0009] The technical solution of this utility model will be described in detail below with reference to the accompanying drawings. Unless otherwise specified, the electrical components, control modules, etc., required for the cell dispensing equipment, as well as their installation or usage methods, are all commercially available products or conventional technical methods.

[0010] This embodiment is a cell dispensing device, including a housing, a mixing zone 1, a dispensing zone, a liquid circuit control module 2, and a refrigeration system.

[0011] like Figures 1 to 4 As shown, the enclosure includes a sheet metal inner shell and a profile frame for supporting the inner shell. The main components of the dispensing equipment are all mounted on the inner shell. The left side of the enclosure has an upper air vent 51 and a lower air vent 52, distributed vertically, to allow cold air from the refrigeration system to enter and maintain a low-temperature environment inside the enclosure. The inner shell is integrally formed from sheet metal, providing a certain degree of airtightness to prevent cold air loss. Its outer surface can be covered with insulation cotton to block heat transfer.

[0012] The mixing zone 1 is located on the upper left side of the inner shell of the box and is equipped with a mixing component. The mixing component can be a commercially available product or a product developed by the company itself, as can be seen in the utility model patent applications with application number 2024216641245 or application number 2025224299288.

[0013] The liquid circuit control module 2 is located on the lower left side of the inner shell of the box. It includes a peristaltic pump (commercially available product with its own motor), a bubble sensor, several clamp valves and other necessary components. These are all commercially available products, and their use and installation are conventional.

[0014] The dispensing area is located on the right side of the inner shell of the container, and features two interconnected sets of "clamping modules 3 + dispensing racks 4" structures arranged vertically, both mounted on the inner shell. Clamping modules 3 are equipped with bubble sensors and several clamping valves, all commercially available products. Clamping modules 3 are used to clamp the infusion tubing to match the dispensing bags on the dispensing racks 4. The dispensing racks 4 are located below the clamping modules 3 and are used to hold the dispensing bags, such as... Figure 1 , Figure 2 and Figure 5 As shown, the assembly includes a base plate 41, a rear plate 43, and several spaced upright plates 42. The upright plates 42 create compartments within the dispensing rack 4, allowing for more even and orderly placement of the dispensing bags and preventing them from crowding together. Both the base plate 41 and the upright plates 42 have several through holes on their surfaces to facilitate vertical and horizontal airflow.

[0015] The refrigeration system adopts a conventional design, including an evaporator assembly 5 (such as a plate-fin evaporator with a built-in fan) for cooling air, a condenser, and a compressor, all of which are commercially available products. Their structure and refrigeration principle are the same as those of household air conditioners. Figure 6 As shown, the control module controls the compressor to compress the refrigerant into a high-temperature, high-pressure gas, which is then cooled by the condenser to become a high-temperature, high-pressure liquid. After being regulated by the expansion valve, it is reduced to a low-temperature, low-pressure liquid. Finally, heat exchange occurs in the evaporator assembly 5, which generates cold air and blows it into the housing. The evaporator assembly 5 is installed on the left side of the housing exterior. Cold air is mainly blown into the packaging area inside the housing through the upper air inlet 51 and the air guide duct 6, and mainly into the mixing area 1 inside the housing through the lower air inlet 52. One end of the air guide duct 6 is connected to the upper air inlet 51, and the other end is located above the packaging area and has several air outlets 61. The air outlets 61 on the air guide duct 6 blow cold air into the packaging area. The two sets of "clamping modules 3 + packaging racks 4" are vertically connected, allowing cold air to also flow downwards. Figures 1 to 4 The mixing zone 1 and the dispensing zone are not separated and are connected. In fact, some of the cold air blown into the mixing zone 1 will also enter the dispensing zone from the side.

[0016] like Figure 3As shown, one end of the air duct 6 covers most of the area connected to the upper air vent 51. The remaining small area of ​​the upper air vent 51 and the entire lower air vent 52 mainly directly supply cold air to the mixing zone 1. This results in a larger volume of cold air in the mixing zone 1, keeping the cell liquid at a relatively low temperature. After mixing, it is transported to the dispensing zone via the liquid path control module 2, thus requiring less cold air. In this embodiment, a thermometer or temperature sensor 7 (specifically, it can be located between the two "clamping modules 3 + dispensing rack 4") can be installed in the dispensing zone to measure the temperature inside the equipment in real time. If a thermometer is used, the start and stop of the refrigeration system can be manually controlled. If the temperature sensor 7 is used, it can be automatically controlled. That is, the temperature sensor 7 feeds back the temperature value to the control module. If the temperature is higher or lower than the set value, the control module will adjust the work of the refrigeration system until the temperature is maintained at the set value. This control process uses conventional principles and methods.

[0017] like Figures 1 to 3 As shown, the condenser and compressor are conventionally installed outside the casing, which can be located at the rear of the inner shell. Similar to household air conditioners, the evaporator assembly 5 typically produces condensate, which can be collected and drained into an external container or a suitable location such as a drain outlet via piping; these are all standard practices.

Claims

1. A cell dispensing device, comprising a housing, a mixing zone (1), a dispensing zone, a liquid path control module (2), and a refrigeration system, characterized in that: It also includes air ducts (6); The mixing zone (1) is located on the upper left side of the box and is equipped with a mixing component. The liquid circuit control module (2) is located on the lower left side of the box. The dispensing zone is located on the right side of the box and is equipped with at least one dispensing component. The box area where the mixing zone (1) is located is equipped with several air inlets to allow the cold air of the refrigeration system to enter. One end of the air duct (6) is connected to one of the air inlets, and the other end is located above the dispensing zone and is equipped with several air outlets (61).

2. The cell dispensing device according to claim 1, characterized in that: The packaging assembly includes a clamping tube module (3) arranged vertically and a packaging rack (4) for placing the packaging bags. The packaging rack (4) includes a bottom plate (41), a back plate (43), and several spaced upright plates (42). The bottom plate (41) and the upright plates (42) are provided with several through holes on their surfaces.

3. The cell dispensing device according to claim 1, characterized in that: The dispensing area is equipped with a temperature sensor (7) or a thermometer.

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