A rapid detection kit for tetracycline resistance

Abstract

The application provides a tetracycline drug resistance rapid detection kit, relates to the technical field of kits, and comprises a kit main body, which contains an inner cavity. Through the mechanical linkage and constraint structure, dynamic self-adaptive fixation of the test tube is realized. When the device is interfered by external force, the internal transmission assembly utilizes inertia to drive the clamping components distributed around to be synchronously folded inward, a flexible radial constraint force is applied through the contact surface with buffering characteristics, and lateral movement of the test tube is effectively prevented. In cooperation with the inner wall guide structure with stepped changes, the design can limit the displacement amplitude of the test tube in the vertical direction, and multi-directional stable protection is formed. Through the joint action of the series of actions, the anti-interference capability of the test tube during operation is significantly improved, and reliable guarantee is provided for smooth development of the detection process.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, and more specifically, to a rapid detection kit for tetracycline resistance. Background Technology

[0002] Current rapid detection methods for tetracycline resistance generally employ immunochromatography. The core principle is the preparation of monoclonal antibodies that specifically bind to tetracycline resistance proteins (such as the Tet efflux pump protein), and the labeling of these antibodies onto tracers such as colloidal gold or colored microspheres. During detection, the lysate of the bacteria to be tested is added to the sample application area of ​​the test strip, and the sample is chromatographically separated along a nitrocellulose membrane by capillary action. If the sample contains the target resistance protein, it will form a complex with the labeled antibody, which will then be intercepted and enriched by another capturing antibody at the detection line, resulting in color development and thus visual interpretation of the resistance. Although this method significantly speeds up the detection compared to traditional drug susceptibility testing, it is essentially still a qualitative or semi-quantitative offline detection technique.

[0003] To fully lyse bacteria and release intracellular proteins, samples need to be pretreated in test tubes containing specific lysis buffers. However, commonly used reagent tubes or test tube racks lack effective fixation structures. When the work surface is slightly bumped or uneven, open reagent tubes are easily tipped over or slipped due to gravity. This movement in the direction of gravity can not only lead to spillage and contamination of precious samples, affecting the accuracy of testing, but also increase operational complexity and biosafety risks in the fast-paced clinical testing environment, thus hindering further improvements in testing efficiency.

[0004] Therefore, we have made improvements to this and proposed a rapid detection kit for tetracycline resistance. Utility Model Content

[0005] To achieve the above-mentioned objectives, this invention provides a rapid detection kit for tetracycline resistance, thereby addressing the aforementioned problems.

[0006] The application is as follows:

[0007] include:

[0008] The reagent kit body includes an inner cavity, and the inner cavity of the reagent kit body has a plurality of spaced-apart limiting members;

[0009] The limiting component includes: a base connected to the bottom surface of the inner cavity, a plurality of support rods arranged around the circumference of the support surface, a pressing block rotatably arranged on the support rod to form a gap for the test tube to pass through, and an inclined surface formed by the gradual narrowing of the channel on the contact surface with the test tube, and a recessed area on the outer ring of the pressing block.

[0010] The suppressing member is spaced apart between adjacent limiting members and has a limiting groove to provide a gravity direction limiting channel;

[0011] A ring-shaped component is disposed around the periphery of the limiting component and connected to the inner cavity of the reagent kit body, and has a pressing component that pulls the ring-shaped component to move along the limiting channel path;

[0012] When the main body of the reagent kit is shaken, the ring device moves toward the tightening pressing block.

[0013] Preferably, the ring component includes:

[0014] A ring sleeve is provided around the periphery of the recess, and the pressing block is provided with an anti-detachment piece to restrict the ring sleeve at the recess.

[0015] A connecting rope connects the adjacent ring sleeves and the ring sleeves at both ends to the inner cavity of the reagent kit body;

[0016] The pressure member is mounted on the connecting rope.

[0017] Preferably, the limiting groove includes an upper section and a lower section, the cross-sectional area of ​​the lower section is larger than the cross-sectional area of ​​the upper section, and a step is formed at the junction of the upper section and the lower section.

[0018] Preferably, there is a gap between the bottom surface of the pressing block and the base.

[0019] Preferably, the contact surface between the pressing block and the test tube is provided with a buffer pad.

[0020] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0021] In the scheme of this application:

[0022] To address the problems in existing technologies, this application achieves dynamic adaptive fixation of the test tube through a mechanical linkage and constraint structure. When the device is subjected to external interference, its internal transmission components utilize inertia to drive the surrounding clamping parts to synchronously retract inward. A flexible radial constraint force is applied through the buffered contact surface, effectively preventing lateral movement of the test tube. Combined with a stepped inner wall guide structure, this design limits the vertical displacement of the test tube, forming multi-directional stability protection. This series of actions works together to significantly improve the test tube's anti-interference capability during operation, providing a reliable guarantee for the smooth implementation of the testing process. Attached Figure Description

[0023] Figure 1 A front view of a rapid detection kit for tetracycline resistance provided in this application;

[0024] Figure 2A schematic diagram of the internal structure of a rapid detection kit for tetracycline resistance provided in this application;

[0025] Figure 3 Exploded view of the limiting components of a rapid detection kit for tetracycline resistance provided in this application;

[0026] Figure 4 A cross-sectional view of the inhibition component of a rapid detection kit for tetracycline resistance provided in this application.

[0027] The image shows:

[0028] 1. Reagent kit body; 2. Restricting component; 21. Base; 22. Support rod; 23. Pressing block; 24. Inclined surface; 25. Recess; 26. Buffer pad; 27. Anti-detachment tablet; 3. Inhibiting component; 30. Restricting groove; 31. Upper section; 32. Lower section; 4. Ring component; 41. Ring sleeve; 42. Connecting rope; 43. Pressing component. Detailed Implementation

[0029] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of the present invention.

[0030] For an example, please refer to... Figure 1 , Figure 2 , Figure 3 and Figure 4 A rapid test kit for tetracycline resistance, comprising:

[0031] The reagent kit body 1 includes an inner cavity, and the inner cavity of the reagent kit body 1 has a plurality of spaced-apart limiting members 2;

[0032] The limiting component 2 includes: a base 21 connected to the bottom surface of the inner cavity, a plurality of support rods 22 arranged around the circumference of the support surface, a pressing block 23 rotatably arranged on the support rod 22 to form a gap for the test tube to pass through, and a slope 24 formed by the gradual narrowing of the channel on the contact surface with the test tube, and a recess 25 is provided on the outer ring of the pressing block 23.

[0033] The suppressing member 3 is spaced between adjacent limiting members 2 and has a limiting groove 30 to provide a gravity direction limiting channel;

[0034] The ring member 4 is arranged around the periphery of the limiting member 2 and connected to the inner cavity of the reagent kit body 1, and has a pressing member 43 on it to pull the ring member 4 to move along the restricted channel path.

[0035] When the reagent kit body 1 shakes, the ring part 4 moves toward the tightening pressing block 23;

[0036] When the equipment is in use, the test tube needs to be passed through the space enclosed by the pressing block 23 so that it can be fixed. During this process, due to the presence of the inclined surface 24, the test tube will gradually come into contact with the pressing block 23 during the pressing process and push the pressing block 23 to rotate, causing it to rotate around the support rod 22. At this time, as the inclined surface 24 gradually adheres to the outer wall of the test tube, the pressing block 23 rotates and gradually opens downwards and moves in the direction of tightening upwards. The gradually tightening part will also have a certain limiting effect on the vertical movement of the test tube.

[0037] according to Figure 2 and Figure 3 It can be observed that under normal conditions, the annular component 4 surrounds the periphery of the pressing block 23. Furthermore, it should be noted that the annular component 4 surrounds the portion of the pressing block 23 that tightens inward during the aforementioned action. Due to the restriction of the anti-detachment piece 27 and the drop in the recess 25, the annular component 4 cannot easily separate from the pressing block 23. Therefore, when the pressing block 23 moves inward, a gradually increasing gap will inevitably form between the pressing block 23 and the annular component 4, causing the pressing component 43 to move downward in the direction of gravity and enter the lower section 32 of the limiting groove 30. This can be determined based on... Figure 4 The pressing member 43 can be a sphere or cube with high-density support. When the pressing member 43 leaves the narrow upper section 31 and enters the wider lower section 32, the pressing member 43 has the ability to swing to both sides. The annular sleeve 41 moves in the direction of narrowing and extending at both ends under the traction of the connecting rope 42. At this time, the annular sleeve 41 achieves the ability to adaptively restrict the test tube.

[0038] In the application scenario of the transfer kit body 1, since the pressing component 43 has the ability to move in the horizontal plane, the pressing component 43 always moves within the space of the lower section 32 during the transfer process. Under the action of lateral acceleration and inertia, the pressing component 43 will move laterally in the lower section 32 to overcome inertia. At this time, the pressing component 43 will further pull the ring sleeve 41 to tighten, so that the pressing block 23 has a pre-tightening force in the direction of squeezing the test tube, which further weakens the shaking ability of the test tube. Moreover, the lateral acceleration and the pre-tightening force are positively correlated. That is, the greater the lateral acceleration, the faster the lateral movement speed, and the stronger the pre-tightening force, which makes the fixation effect on the test tube better.

[0039] In some embodiments, the annular portion of the ring sleeve 41 has a hollow cavity, and the connecting rope 42 passes through the cavity of the ring sleeve 41 and connects to both ends of the inner cavity of the reagent kit body 1. In this state, the tearing force in the downward direction caused by the direct fixed connection of the connecting rope 42 to the ring sleeve 41 can be reduced, which would cause the structure of the ring sleeve 41 to be damaged under long-term use, thus extending the service life of the device.

[0040] Ring component 4 includes:

[0041] A ring sleeve 41 is provided around the periphery of the recess 25, and a pressing block 23 is provided at the recess 25 with an anti-detachment piece 27 that restricts the ring sleeve 41.

[0042] The connecting rope 42 connects to the adjacent ring sleeve 41 and the inner cavity of the ring sleeve 41 at both ends and the main body 1 of the reagent kit;

[0043] The pressure member 43 is mounted on the connecting rope 42;

[0044] Multiple ring sleeves 41 are connected in series by connecting ropes 42, and pressure members 43 are set at multiple ends. When the reagent kit is shaken, the inertial displacement of the pressure members 43 is synchronously transmitted to all ring sleeves 41 through the connecting ropes 42, so that the restrictors 2 distributed in different positions can act simultaneously and tighten uniformly towards the center, thereby achieving a uniform and synchronous fixation effect on multiple test tubes.

[0045] The limiting groove 30 includes an upper section 31 and a lower section 32. The cross-sectional area of ​​the lower section 32 is larger than that of the upper section 31. The upper section 31 and the lower section 32 form a step at their junction. The step structure at their junction can effectively limit and block the flow of energy, prevent the test tube from moving excessively in the direction of gravity or even falling out, and prevent the pressing component 43 from entering the upper section 31.

[0046] There is a gap between the bottom surface of the pressing block 23 and the base 21, which provides space for the pressing block 23 to rotate.

[0047] A buffer pad 26 is provided on the contact surface between the pressing block 23 and the test tube to prevent the contact surface of the test tube from breaking due to the pre-tightening force.

[0048] The technical scope of this utility model is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this utility model, and all such modifications and variations should fall within the protection scope of this utility model.

Claims

1. A rapid detection kit for tetracycline resistance, characterized in that, include: The reagent kit body (1) includes an inner cavity, and the inner cavity of the reagent kit body (1) has a plurality of spaced-apart limiting members (2). The limiting member (2) includes: a base (21) connected to the bottom surface of the inner cavity, the base (21) having a plurality of support rods (22) arranged around the circumference of the support surface, a pressing block (23) rotatably arranged on the support rod (22) to form a gap for the test tube to pass through, the contact surface between the pressing block (22) and the test tube having a slope (24) formed by the channel gradually narrowing, and the outer ring of the pressing block (23) having a recess (25). The suppressing member (3) is spaced between adjacent restricting members (2) and has a restricting groove (30) to provide a gravity direction restricting channel; The ring (4) is arranged around the periphery of the limiting member (2) and connected to the inner cavity of the reagent kit body (1), and has a pressing member (43) that pulls the ring (4) to move along the limiting channel path. When the reagent kit body (1) shakes, the ring part (4) moves toward the tightening pressing block (23).

2. The rapid detection kit for tetracycline resistance according to claim 1, characterized in that, The ring element (4) includes: A ring sleeve (41) is provided around the periphery of the recess (25), and the pressing block (23) is provided with an anti-detachment piece (27) to restrict the ring sleeve (41) at the recess (25). A connecting rope (42) is connected between the adjacent ring sleeves (41) and the inner cavity of the ring sleeves (41) at both ends and the body of the reagent kit (1); The pressing component (43) is mounted on the connecting rope (42).

3. The tetracycline resistance rapid detection kit according to claim 2, characterized in that, The limiting groove (30) includes an upper section (31) and a lower section (32), the cross-sectional area of ​​the lower section (32) is larger than the cross-sectional area of ​​the upper section (31), and a step is formed at the junction of the upper section (31) and the lower section (32).

4. The rapid detection kit for tetracycline resistance according to claim 3, characterized in that, There is a gap between the bottom surface of the pressing block (23) and the base (21).

5. The rapid detection kit for tetracycline resistance according to claim 4, characterized in that, The pressing block (23) is provided with a buffer pad (26) on the contact surface with the test tube.

Images