A pour back device for viral inactivation solution processing

Abstract

The utility model discloses a kind of for virus inactivation liquid processing's pour tank device, comprising: virus liquid receiving barrel and the barrel cover located on the upper end of the virus liquid receiving barrel, connecting port is equipped on the barrel cover;The connecting port includes first connecting port, second connecting port, third connecting port, the virus liquid receiving barrel is connected by the first connecting port with compressed air or vacuum source;The virus liquid receiving barrel is respectively connected with beta-propiolactone glass bottle, inactivation virus liquid receiving barrel by the second connecting port;The virus liquid receiving barrel is connected with virus liquid original bottle by the third connecting port. By the above mode, the utility model changes the traditional inactivation and pour tank mode, effectively avoids the pollution risk possibly caused due to virus liquid inactivation not thoroughly in pour tank process, greatly improves the security of production process.

Description

Technical Field

[0001] This utility model relates to the field of biopharmaceutical equipment technology, and in particular to a tank-switching device for treating virus inactivation solution. Background Technology

[0002] In the production of viral vaccine bulk solutions, hemorrhagic fever virus fluid is typically collected in stainless steel containers. To inactivate the virus harvest fluid, a common method involves connecting a sterile respirator to the container opening and using the negative pressure created by the vacuum to draw the inactivating agent β-propiolactone into the stainless steel container. Specifically, the silicone tubing connecting the β-propiolactone aspiration tube must extend to the bottom of the virus harvest fluid container to ensure sufficient contact between the inactivating agent and the virus harvest fluid. However, because the silicone tubing extends to the bottom of the container, the virus harvest fluid can easily contaminate the container walls or top, making it impossible to guarantee complete inactivation of the virus in these areas. This poses a potential risk to the production process; if this incompletely inactivated virus bulk solution leaks or spreads, it will threaten production safety and personnel health. Utility Model Content

[0003] The purpose of this invention is to provide a simple and easy-to-operate inverting device for effectively inactivating virus harvesting liquid, preventing the risk of incomplete virus inactivation caused by contamination of the tank wall or top of the virus harvesting liquid, and ensuring the safety of the production process and product quality.

[0004] To achieve the above objectives, the present invention provides the following technical solution: A transfer device for treating virus inactivation solution includes: a virus collection tank and a lid located at the upper end of the virus collection tank, with a connection port provided on the lid; The connection ports include a first connection port, a second connection port, and a third connection port. The virus collection container is connected to a compressed air or vacuum source through the first connection port. The virus collection container is connected to the β-propiolactone glass bottle and the inactivated virus collection container respectively through the second connection port; The virus collection container is connected to the original virus liquid bottle via the third connection port.

[0005] Preferably, one end of the first connecting tube is inserted into the virus collection tank through the first connection port, and the other end extends to the outside of the virus collection tank and is connected to compressed air or a vacuum source through a No. 1 silicone tube. An air filter element for filtering compressed gas is provided on the first silicone tube.

[0006] Preferably, one end of the second connecting tube is inserted through the second connection port into the bottom of the virus collection tank, and the other end extends to the outside of the virus collection tank and is connected to the second silicone tube. The second silicone tube is then connected to the β-propiolactone glass bottle, the inactivated virus collection tank, and the sampling bottle, respectively.

[0007] Preferably, the second silicone tube is divided into a first passage and a second passage by a three-way connector; The first access port connects to the β-propiolactone glass bottle and the inactivated virus collection container via the No. 4 silicone tube, respectively. The second access port is connected to the sampling bottle via a No. 5 silicone tube, and a control valve is provided between the second access port and the sampling bottle.

[0008] Preferably, the first access port divides the fourth silicone tube into a third access port and a fourth access port through a three-way connector; The third access port is connected to the β-propiolactone glass bottle via a No. 6 silicone tube, and a control valve is provided between the two connections; the fourth access port is connected to the inactivated virus collection tank via a No. 7 silicone tube, and a control valve is provided between the two connections.

[0009] Preferably, one end of the third connecting tube is inserted into the virus collection tank through the third connecting port, and the other end extends to the outside of the virus collection tank and is connected to the original virus liquid bottle through the No. 3 silicone tube.

[0010] Preferably, the upper end of the sampling bottle is provided with a sampling cover to prevent the sampling bottle from being contaminated.

[0011] Preferably, the first connection port, the second connection port, and the third connection port are all connected to the bucket lid by a stainless steel quick chuck.

[0012] Preferably, the pressure adjustment range of the compressor is positive pressure 0.05~0.09MPa and negative pressure -0.08~-0.09MPa.

[0013] The beneficial effects of this utility model are: This utility model discloses a transfer device for treating virus inactivation solution, comprising a virus collection tank and a lid located at the top of the virus collection tank. The lid has a first connection port, a second connection port, and a third connection port. The virus collection tank is connected to a compressed air or vacuum source through the first connection port. The virus collection tank is connected to a β-propiolactone glass bottle and an inactivated virus collection tank through the second connection port. The virus collection tank is connected to the original virus solution bottle through the third connection port. Under the positive / negative pressure of the compressor, the collection tank can perform inactivation by adding the extinguishing agent β-propiolactone, sampling the inactivated virus solution, and transferring the inactivated virus solution. All these operations are completed in the pipeline channel, reducing human operation and exposure of the virus solution to the environment. This effectively prevents the risk of incomplete inactivation caused by virus solution contamination on the tank wall or top of the collection tank, ensuring the safety of the production process and product quality. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of a preferred embodiment of a tank-turning device for treating virus inactivation solution according to the present invention; The components in the attached diagram are labeled as follows: 1. Virus collection container; 1-1. Container lid; 2. Inactivated virus collection container; 3. Original virus solution bottle; 4. Sampling bottle; 5. β-propiolactone glass bottle; 6. First connection port; 7. Second connection port; 8. Third connection port; 9. First connecting tube; 10. Second connecting tube; 11. Third connecting tube; 12. Silicone tube No. 1; 13. Silicone tube No. 2; 13-1. First access port; 13-2. Second access port; 14. Silicone tube No. 3; 15. T-connector; 16. Silicone tube No. 4; 16-1. Third access port; 16-2. Fourth access port; 17. Silicone tube No. 5; 18. Silicone tube No. 6; 19. Silicone tube No. 7; 20. Sampling hood; 21. Air filter. Detailed Implementation

[0015] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the scope of protection of the present invention.

[0016] Example: This embodiment describes the structure of a transfer device for treating virus inactivation solution.

[0017] Combined with appendix Figure 1 As shown, Figure 1 This is a schematic diagram of a preferred embodiment of a tank-turning device for treating virus inactivation solution according to the present invention. The tank-turning device for treating virus inactivation solution includes: a virus collection tank 1 and a tank cover 1-1 located at the upper end of the virus collection tank 1, with a connection port provided on the tank cover 1-1; The connection ports include a first connection port 6, a second connection port 7, and a third connection port 8. All three ports are connected to the lid 1-1 with a stainless steel quick chuck.

[0018] Connect the virus collection container 1 to a compressed air or vacuum source via the first connection port 6. The virus collection container 1 is connected to the β-propiolactone glass bottle 5 and the inactivated virus collection container 2 respectively through the second connection port 7. Connect the virus collection container 1 to the original virus liquid bottle 3 via the third connection port 8.

[0019] In this embodiment, the virus collection tank, the tank lid, and the inactivated virus collection tank are all made of 316 stainless steel.

[0020] In this embodiment, the first, second, and third connection ports are each connected to a connecting pipe to form three large passageways with different functions. Specifically: One end of the first connecting tube 9 is inserted into the virus collection tank 1 through the first connecting port 6, and the other end extends to the outside of the virus collection tank 1 and is connected to compressed air or a vacuum source through the first silicone tube 12.

[0021] The virus collection tank is connected to a compressed air system. During the transfer process, positive pressure forces the inactivated virus from the virus collection tank into the inactivated virus collection tank 2. The virus collection tank is connected to a vacuum source, with a vacuum system providing the vacuum. Negative pressure draws in the original virus solution or the inactivating agent β-propiolactone. Additionally, pressure adjustment before transfer can also be achieved by connecting compressed air or a vacuum system. If pressure balancing is required during or after transfer, a vacuum source can be connected to help release pressure and vent the tank.

[0022] In a preferred embodiment, an air filter element 21 is also provided on the first silicone tube 12, the function of which is to filter impurities in the compressed gas.

[0023] Furthermore, one end of the second connecting tube 10 is inserted through the second connecting port 7 to the bottom of the virus collection tank 1, and the other end extends to the outside of the virus collection tank 1 and connects to the second silicone tube 13. The second silicone tube 13 is then connected to the β-propiolactone glass bottle 5, the inactivated virus collection tank 2, and the sampling bottle 4, respectively. Through the above connections, a passage is formed between the pipelines.

[0024] The function of connecting the second silicone tube 13 to the β-propiolactone glass bottle 5 is: to connect the virus collection tank to the vacuum source and draw the β-propiolactone prepared in the β-propiolactone glass bottle into the virus collection tank 1 under the action of vacuum negative pressure. The function of connecting the No. 2 silicone tube 13 to the inactivated virus collection tank 2 is: the virus collection tank is connected to compressed air, and the virus solution in the virus collection tank is inactivated and then transferred to the inactivated virus collection tank 2 through the No. 2 silicone tube. The purpose of connecting the second silicone tube 13 to the sampling bottle 4 is to sample the virus solution in the virus collection tank during the production process for subsequent sampling and testing.

[0025] One end of the third connecting tube 11 is inserted into the virus collection tank 1 through the third connecting port 8, and the other end extends to the outside of the virus collection tank 1 and is connected to the original virus solution bottle 3 through the third silicone tube 14. This connection forms a passage between the pipes. The function of the third silicone tube 14 connected to the original virus solution bottle 3 is to draw the virus solution from the original bottle into the virus collection tank 1 under vacuum negative pressure. A control valve is installed between the third silicone tube 14 and the original virus solution bottle 3 to control the drawing of the virus solution from the original bottle into the virus collection tank.

[0026] In the preferred embodiment, based on the function of the pathway formed by the No. 2 silicone tube, the connections between the No. 2 silicone tube and the β-propiolactone glass bottle 5, the inactivated virus collection container 2, and the sampling bottle 4 are refined to achieve small pathways between the No. 2 silicone tube and the β-propiolactone glass bottle, between the No. 2 silicone tube and the inactivated virus collection container, and between the No. 2 silicone tube and the sampling bottle, which can satisfy each function without interfering with each other. In this utility model, a three-way connector 15 is set to divide the No. 2 silicone tube 13 into different passage ports, namely the first passage port 13-1 and the second passage port 13-2. The first passage port 13-1 is connected to the β-propiolactone glass bottle 5 and the inactivated virus collection container 2 through the No. 4 silicone tube 16; the second passage port 13-2 is connected to the sampling bottle 4 through the No. 5 silicone tube 17, and a control valve is set between the second passage port 13-2 and the sampling bottle 4 to close or open the pathway formed between the second passage port and the sampling bottle when needed.

[0027] Furthermore, the connection between silicone tube 2 (No. 2), the β-propiolactone glass bottle 5, and the inactivated virus collection container 2 are further divided to ensure the function of each pathway. Based on the foregoing, the first pathway 13-1 is also divided into a third pathway 16-1 and a fourth pathway 16-2 via a T-joint 15. The third pathway 16-1 is connected to the β-propiolactone glass bottle 5 via silicone tube 18 (No. 6), and the fourth pathway 16-2 is connected to the inactivated virus collection container 2 via silicone tube 19 (No. 7). To ensure that the silicone tube 2, the β-propiolactone glass bottle, and the inactivated virus collection container are interconnected but do not interfere with each other, control valves are installed between silicone tube 18 (No. 6) and the β-propiolactone glass bottle 5, and between silicone tube 18 (No. 6) and the inactivated virus collection container 2. The control valve can be closed or opened in the passageway formed between the No. 6 silicone tube, the β-propiolactone glass bottle, and the inactivated virus collection container.

[0028] In this embodiment, the control valve mentioned in the above scheme is illustrated using hemostatic forceps.

[0029] The tee connector is used for liquid / gas in diversion or merging pipelines. It is made of polypropylene (PP). In the inverted container device of this utility model, the tee connector is used to connect the silicone tube to realize multi-channel control of inactivator addition, inverted container and sampling.

[0030] In this embodiment, a sampling cover 20 is provided at the upper end of the sampling bottle 4. The dispensing cover covers the sampling bottle to prevent the sampling bottle from being contaminated and to ensure sterile sampling. The sampling cover 20 is made of 316 stainless steel.

[0031] Working principle: Preparation of Virus Inactivation Solution: Prepare Virus Collection Tank 1. Close the control valves on silicone tubes 16 (No. 4) and 17 (No. 5). Connect silicone tube 12 (No. 1) to a vacuum source with a vacuum pressure range of -0.08 to -0.09 MPa. At this time, silicone tube 14 (No. 3) is connected to the original virus solution bottle 3. Using the negative pressure generated by the vacuum, the virus solution in the original bottle is drawn into the stainless steel collection tank 1. Next, close the control valve between silicone tube 14 (No. 3) and the original virus solution bottle 3, open the control valve on silicone tube 16 (No. 4), and close the control valve on silicone tube 19 (No. 7). Using the negative pressure generated by the vacuum, the prepared inactivating agent β-propiolactone in glass bottle 5 is drawn into the virus collection tank 1. After the aspiration is complete, place the collection tank on a portable magnetic stirrer and turn on the magnetic stirrer at a speed of 600 rpm to thoroughly stir the liquid in the tank, ensuring that the virus solution and β-propiolactone are evenly mixed to form the virus inactivation solution.

[0032] In this process, this invention uses vacuum negative pressure to draw the virus liquid into the virus collection tank. Each virus liquid bottle originally contains 400ml of virus liquid. Using the above method, 10 bottles of virus liquid can be drawn in one minute, which is fast and sterile. In actual operation, because a few operations need to collect 150 virus liquid bottles, the traditional method of collecting liquid involves manually pouring liquid from each bottle into the virus collection tank. This operation is cumbersome, and the virus liquid is exposed to air multiple times, posing a significant risk to sterility and causing great safety hazards to personnel and the environment. Therefore, the operation method of this invention is simpler, reduces manual intervention, and ensures the sterility of the virus harvested liquid.

[0033] Transferring the inactivated virus solution: After stirring, connect virus collection tank 1 and inactivated virus collection tank 2 via silicone tubing. Close the control valve on silicone tubing 18 (number 6) and open the control valve on silicone tubing 19 (number 7). Connect silicone tubing 12 to compressed air and open the air compressor system valve. Use positive pressure to force the inactivated virus solution into inactivated virus collection tank 2. The air compressor system should be set to 0.05-0.09 MPa. During the transfer process, closely monitor the liquid flow and pressure changes to ensure a smooth transfer.

[0034] Sampling: Close the control valves on silicone tube 16 (No. 4) and silicone tube 14 (No. 3). Connect silicone tube 12 to the compressed air system. Set the compressed air system pressure range to 0.05-0.09 MPa. Compressed air enters the virus collection tank 1, and the virus inactivation solution is forced into the sampling bottle 4 through silicone tube 17 (No. 5) for sampling. This method allows for accurate acquisition of representative samples without affecting the overall environment inside the tank, facilitating various sampling and testing processes and ensuring product quality meets standards.

[0035] By adopting the above technical solutions, the following effects have already been achieved in actual production and use: (1) Avoiding the risk of contamination: The virus liquid is collected and inactivated by this transfer device, which changes the traditional inactivation and transfer method. It effectively avoids the risk of contamination caused by incomplete inactivation of the virus liquid during the transfer process, and greatly improves the safety of the production process.

[0036] (2) Easy to operate: The design of this device makes it easier for personnel to operate. They only need to connect the corresponding pipelines according to the established process and control the entry of compressed air or vacuum to complete the inactivation and transfer process, which reduces the difficulty of operation and the probability of error for operators and improves the efficiency of operation.

[0037] (3) Ensure sterile production: The design of the entire device fully considers the requirements of sterile control. The various connection ports and pipelines are set up reasonably. While ensuring the smooth operation of the production process, it minimizes the possibility of external factors contaminating the liquid in the tank, thus effectively ensuring sterile control. Compared with the existing operation method, this greatly reduces the safety risks to personnel and the environment during operation.

[0038] (4) Complete inactivation of the virus: Using the above-mentioned scheme of this utility model, the hemorrhagic fever virus harvest liquid is added to β-propiolactone and stirred evenly before being transferred to another container, which can ensure that the virus is completely inactivated. After being transferred, the empty container of harvest liquid is transferred out of the clean area after being inactivated by moist heat at 100°C. After actual production verification, after using this transfer device, samples of each container of virus inactivation liquid were taken to confirm the inactivation effect, and the test results were all negative, which fully ensured the safety of the production process.

[0039] This utility model device has an ingenious overall design, simple structure, and easy operation, which reduces production costs while improving production efficiency.

[0040] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A transfer device for treating virus inactivation solution, characterized in that, include: The virus collection tank (1) and the lid (1-1) located at the upper end of the virus collection tank (1) are provided with a connection port on the lid (1-1); The connection ports include a first connection port (6), a second connection port (7), and a third connection port (8). The virus collection tank (1) is connected to a compressed air or vacuum source through the first connection port (6); The virus collection container (1) is connected to the β-propiolactone glass bottle (5) and the inactivated virus collection container (2) respectively through the second connection port (7). The virus collection tank (1) is connected to the virus liquid original bottle (3) through the third connection port (8).

2. The transfer device for treating virus inactivation solution according to claim 1, characterized in that: One end of the first connecting tube (9) is fed into the virus collection tank (1) through the first connecting port (6), and the other end extends to the outside of the virus collection tank (1) and is connected to compressed air and vacuum source through the first silicone tube (12). An air filter (21) for filtering compressed gas is provided on the first silicone tube (12).

3. The transfer device for treating virus inactivation solution according to claim 1, characterized in that: One end of the second connecting tube (10) is inserted through the second connecting port (7) to the bottom of the virus collection tank (1), and the other end extends to the outside of the virus collection tank (1) and is connected to the second silicone tube (13). The second silicone tube (13) is then connected to the β-propiolactone glass bottle (5), the inactivated virus collection tank (2), and the sampling bottle (4) respectively.

4. A transfer device for treating virus inactivation solution according to claim 3, characterized in that: The second silicone tube (13) is divided into a first access port (13-1) and a second access port (13-2) by a three-way connector (15). The first access port (13-1) is connected to the β-propiolactone glass bottle (5) and the inactivated virus collection container (2) respectively through the No. 4 silicone tube (16); The second access port (13-2) is connected to the sampling bottle (4) via a No. 5 silicone tube (17), and a control valve is provided between the second access port (13-2) and the sampling bottle (4).

5. A transfer device for treating virus inactivation solution according to claim 4, characterized in that: The first access port (13-1) divides the fourth silicone tube (16) into a third access port (16-1) and a fourth access port (16-2) through a three-way connector (15). The three-way port (16-1) is connected to the β-propiolactone glass bottle (5) via a No. 6 silicone tube (18), and a control valve is provided between the connections; the fourth-way port (16-2) is connected to the inactivated virus collection tank (2) via a No. 7 silicone tube (19), and a control valve is provided between the connections.

6. A transfer device for treating virus inactivation solution according to claim 1, characterized in that: One end of the third connecting tube (11) is inserted into the virus collection tank (1) through the third connecting port (8), and the other end extends to the outside of the virus collection tank (1) and is connected to the original virus liquid bottle (3) through the No. 3 silicone tube (14).

7. A transfer device for treating virus inactivation solution according to claim 3, characterized in that: The upper end of the sampling bottle (4) is provided with a sampling cover (20) to prevent the sampling bottle from being contaminated.

8. A transfer device for treating virus inactivation solution according to claim 1, characterized in that: The first connection port (6), the second connection port (7), and the third connection port (8) are all sealed to the barrel lid (1-1) using stainless steel quick chucks.

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