A laboratory small solid fermentation device for biocontrol fungi

Abstract

The utility model discloses a kind of biocontrol fungi laboratory small solid fermentation devices, belong to fungi experimental fermentation technical field, this one kind of biocontrol fungi laboratory small solid fermentation device includes experimental fermentation box, the biocontrol fungi solid placing mechanism is installed in experimental fermentation box one side, experimental fermentation box one end is installed with oxygen supply mechanism, the biocontrol fungi solid placing mechanism includes mounting plate, the mounting plate is installed in experimental fermentation box one side, motor is installed in mounting plate one side. The device is contacted more evenly by biocontrol fungi solid placing mechanism with material and gas, ensure that mycelium grows evenly, so that fermentation test is more accurate, by oxygen supply mechanism, while oxygen supply, the gas that needs to be discharged can be carried out multi-level and comprehensive purification work, prevent gas damage health of operator and prevent laboratory environment cross-contamination.

Description

Technical Field

[0001] This utility model relates to the field of experimental fermentation of fungi, and more specifically, to a small-scale solid fermentation device for biocontrol fungi in the laboratory. Background Technology

[0002] Small-scale solid-state fermentation devices for biocontrol fungi are specialized equipment used in laboratories for small-scale cultivation of biocontrol fungi (such as Beauveria bassiana, Metarhizium anisopliae, Trichoderma, etc.). They are primarily used to optimize fermentation processes and produce small quantities of active spores (or mycelium) to provide materials for subsequent experiments such as biocontrol efficacy testing, pathogenicity studies, and fermentation parameter screening. Existing small-scale solid-state fermentation devices for biocontrol fungi have certain shortcomings. Most of these devices employ static cultivation methods, where the fungal solid material remains in a static state for extended periods. This leads to significant differences in internal permeability and uneven distribution of oxygen and nutrients, resulting in inconsistent mycelial growth and fluctuating spore production, thus reducing the accuracy of experimental results. Furthermore, as aerobic microorganisms, biocontrol fungi require continuous oxygen supply and timely removal of mixed gases containing fungal spores and metabolic waste during fermentation. Existing small-scale solid-state fermentation devices often directly release these gases into the laboratory, potentially harming the health of operators and causing cross-contamination of the laboratory environment. Addressing these issues has become a pressing problem for those skilled in the art. Utility Model Content

[0003] To overcome the above shortcomings, this utility model provides a small-scale solid-state fermentation device for biocontrol fungi in laboratories. The aim is to address certain deficiencies in existing small-scale solid-state fermentation devices for biocontrol fungi. Existing such devices mostly employ static cultivation methods, where the fungal solid material remains in a static state for extended periods. This leads to significant differences in internal permeability and uneven distribution of oxygen and nutrients, resulting in inconsistent mycelial growth and fluctuating spore production, thus reducing the accuracy of experimental results. Furthermore, as aerobic microorganisms, biocontrol fungi require continuous oxygen supply and timely removal of mixed gases containing fungal spores and metabolic waste during fermentation. Existing small-scale solid-state fermentation devices for biocontrol fungi often directly release the gases produced during operation into the laboratory, potentially harming the health of operators and easily causing cross-contamination of the laboratory environment.

[0004] This invention is implemented as follows: a small solid-state fermentation device for biocontrol fungi in the laboratory, comprising an experimental fermentation chamber, a solid-state placement mechanism for biocontrol fungi installed on one side of the experimental fermentation chamber, and an oxygen supply mechanism installed at one end of the experimental fermentation chamber.

[0005] In a preferred embodiment of this invention, the solid placement mechanism for biocontrol fungi includes a mounting plate mounted on one side of the experimental fermentation chamber. A motor is mounted on one side of the mounting plate, and a rotating rod is rotatably mounted on the other side of the mounting plate. The rotating rod is driven by the motor. A rotating plate is mounted at one end of the rotating rod, and a connecting rod is hinged to a non-central position on one side of the rotating plate. A movable rod is hinged to one end of the connecting rod, and the movable rod slides and seals through the experimental fermentation chamber. A movable seat is mounted at one end of the movable rod, and a placement box is detachably mounted on the upper end of the movable seat. A protruding strip is provided inside the placement box to hold the material to be fermented. Solid fungi, such as Beauveria bassiana, Metarhizium anisopliae, and Trichoderma, are placed in a placement box. The placement box is detachably mounted on a movable base for easy removal and placement from the fermentation chamber. A motor drives a rotating rod, which in turn rotates a rotating plate, causing a connecting rod to move a movable rod. Simultaneously, the movable rod moves laterally in conjunction with the fermentation chamber, causing the material in the placement box to be agitated by protruding strips during this lateral movement. This prevents the material from remaining in a uniform state within the fermentation chamber, which could lead to uneven contact between the material and gas and affect the accuracy of the fermentation experiment, thus ensuring uniform mycelial growth.

[0006] In a preferred embodiment of this utility model, a connecting strip is installed at the bottom of the electric placement box, and a connecting groove is provided at the upper end of the movable seat in conjunction with the connecting strip. A plate is installed at one end of the connecting strip, and threaded grooves are provided on one side of the plate and the movable seat and are fixed by bolts. The placement box is initially connected by the connecting strip and the connecting groove, and then fixed by the plate and the bolts. The bolts are made of anti-oxidation material.

[0007] In a preferred embodiment of this utility model, a guide block is installed at the bottom of the movable seat, and a guide groove is provided at the inner end of the experimental fermentation chamber, with the guide block slidably connected to the guide groove.

[0008] In a preferred embodiment of this utility model, a second external support is installed on one side of the placement box, and the protruding strip is set in an arc shape.

[0009] In a preferred embodiment of this utility model, the oxygen supply mechanism includes a pipe body. One end of the experimental fermentation chamber is connected to the pipe body and a connecting pipe. The experimental fermentation chamber is connected to an external oxygen supply device through the pipe body. A purification chamber is installed at the upper end of the experimental fermentation chamber. The purification chamber and the connecting pipe are connected. The inner wall of the purification chamber is equipped with a HEPA filter membrane purification plate, an activated carbon composite purification plate, and a ceramic filter purification plate. An exhaust pipe is connected to the upper end of the purification chamber. Biocontrol fungi are aerobic microorganisms; their growth and spore production require a continuous oxygen supply, and they also need to exhaust some gases that require purification, such as CO2 produced during metabolism. To protect the health of operators and prevent cross-contamination of the laboratory environment, the fungal spores and bacteria and molds produced during fermentation are purified through multiple layers of purification using HEPA filter membranes, activated carbon composite purification plates, and ceramic filter purification plates. During operation, an external oxygen supply device is connected through a pipe to supply oxygen to the fungal solid material in the experimental fermentation chamber. To ensure the air pressure inside the experimental fermentation chamber and to remove some gases that need purification, such as CO2 produced during metabolism, the gases enter the purification chamber through a connecting pipe and are purified by the HEPA filter membranes, activated carbon composite purification plates, and ceramic filter purification plates. After purification, the gases are discharged through an exhaust pipe.

[0010] In a preferred embodiment of this utility model, the pipe body is provided with a solenoid valve, and the connecting pipe is provided with a second solenoid valve.

[0011] In a preferred embodiment of this utility model, the experimental fermentation chamber has an opening on one side, a door is sealed and hinged on one side of the experimental fermentation chamber, an external support is installed on one side of the door, and a heater is provided inside the experimental fermentation chamber.

[0012] The beneficial effects of this utility model are as follows: This utility model provides a small-scale solid-state fermentation device for biocontrol fungi in a laboratory. During use, the solid fungi to be fermented are placed in a placement box. The box is initially connected using connecting strips and connecting grooves, and then fixed using bolts. The door is then closed. When oxygen supply is needed, an external oxygen supply device is connected through a pipe to supply oxygen to the solid fungi in the fermentation chamber. When gas needs to be discharged, the gas enters the purification chamber through a connecting pipe and undergoes multi-level and comprehensive purification by HEPA filter membrane purification plates, activated carbon composite purification plates, and ceramic filter purification plates. Simultaneously, a motor drives a rotating rod, causing the rotating plate to rotate, which in turn causes the connecting rod to move the moving rod. The moving rod, in conjunction with the fermentation chamber, moves laterally, causing the material in the placement box to be agitated by the protruding strips during this lateral movement. This ensures more uniform contact between the material and gas, guaranteeing uniform mycelial growth. This device uses a solid placement mechanism for biocontrol fungi to ensure more uniform contact between materials and gases, guaranteeing uniform mycelial growth and making fermentation experiments more accurate. The oxygen supply mechanism can simultaneously supply oxygen and perform multi-level and comprehensive purification of the gases to be discharged, preventing gas from harming the health of operators and preventing cross-contamination of the laboratory environment. Attached Figure Description

[0013] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of a small-scale solid-state fermentation device for biocontrol fungi in a laboratory, provided by an embodiment of this utility model.

[0015] Figure 2 Another structural schematic diagram provided for an embodiment of this utility model;

[0016] Figure 3 A schematic diagram of the internal structure provided for an embodiment of this utility model;

[0017] Figure 4 Another internal structure diagram provided for an embodiment of this utility model.

[0018] In the diagram: 100-Experimental fermentation chamber; 101-Guide groove; 110-Heater; 120-Door; 121-External support; 200-Solid placement mechanism for biocontrol fungi; 210-Mounting plate; 220-Motor; 230-Rotating rod; 240-Rotating plate; 250-Connecting rod; 260-Moving rod; 270-Moving seat; 271-Guide block; 280-Placement box; 281-Connecting strip; 282-Plate body; 283-Bolt; 284-Second external support; 290-Protruding strip; 300-Oxygen supply mechanism; 310-Pipe body; 311-Solenoid valve; 320-Connecting pipe; 321-Second solenoid valve; 330-Purification chamber; 340-HEPA filter membrane purification plate; 350-Activated carbon composite purification plate; 360-Ceramic filter purification plate; 370-Exhaust pipe. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0020] Please see Figures 1-4 The present invention provides a technical solution: a small solid fermentation device for biocontrol fungi in laboratory, including an experimental fermentation box 100, a solid placement mechanism for biocontrol fungi 200 installed on one side of the experimental fermentation box 100, and an oxygen supply mechanism 300 installed at one end of the experimental fermentation box 100.

[0021] In some specific implementation schemes, the biocontrol fungi solid placement mechanism 200 includes a mounting plate 210, which is installed on one side of the experimental fermentation chamber 100. A motor 220 is installed on one side of the mounting plate 210, and a rotating rod 230 is rotatably installed on the other side of the mounting plate 210. The rotating rod 230 is driven by the motor 220. A rotating plate 240 is installed at one end of the rotating rod 230. A connecting rod 250 is hinged to a non-central position on one side of the rotating plate 240. A moving rod 260 is hinged to one end of the connecting rod 250. The moving rod 260 slides and seals through the experimental fermentation chamber 100. A moving seat 270 is installed at one end of the moving rod 260. A placement box 280 is detachably installed on the upper end of the moving seat 270. A protruding strip 290 is provided inside the placement box 280 to hold the fungal solids to be fermented, such as... Solid Beauveria bassiana, Metarhizium anisopliae, and Trichoderma are placed in placement box 280, which is detachably mounted on movable seat 270 for easy removal and placement from the experimental fermentation chamber 100. This facilitates material removal and placement. Motor 220 drives rotating rod 230, which in turn rotates rotating plate 240, causing connecting rod 250 to move moving rod 260. Simultaneously, moving rod 260 moves laterally in conjunction with the experimental fermentation chamber 100, causing the material in placement box 280 to be agitated by protruding strip 290 during this lateral movement. This prevents the material from remaining in a uniform state within the fermentation chamber 100, which could lead to uneven contact between material and gas and affect the accuracy of the fermentation experiment, thus ensuring uniform mycelial growth.

[0022] In some specific implementation schemes, a connecting strip 281 is installed at the bottom of the electric placement box 280, and a connecting groove is provided at the upper end of the movable seat 270 to cooperate with the connecting strip 281. A plate 282 is installed at one end of the connecting strip 281. The plate 282 and the movable seat 270 are provided with threaded grooves on one side and are fixed by bolts 283. The placement box 280 is initially connected by the connecting strip 281 and the connecting groove, and then fixed by the plate 282 and the bolts 283. The bolts 283 are made of anti-oxidation material.

[0023] In some specific implementation schemes, a guide block 271 is installed at the bottom of the movable seat 270, and a guide groove 101 is provided at the inner end of the experimental fermentation box 100, with the guide block 271 slidably connected to the guide groove 101.

[0024] In some specific implementations, a second external support 284 is installed on one side of the placement box 280, and the protruding strip 290 is set in an arc shape.

[0025] In some specific implementation schemes, the oxygen supply mechanism 300 includes a pipe body 310. One end of the experimental fermentation chamber 100 is connected to the pipe body 310 and a connecting pipe 320. The experimental fermentation chamber 100 is connected to an external oxygen supply device through the pipe body 310. A purification chamber 330 is installed at the top of the experimental fermentation chamber 100, and the purification chamber 330 is connected to the connecting pipe 320. The inner wall of the purification chamber 330 is equipped with a HEPA filter membrane purification plate 340, an activated carbon composite purification plate 350, and a ceramic filter purification plate 360. An exhaust pipe 370 is connected to the top of the purification chamber 330. Biocontrol fungi are aerobic microorganisms; their growth and spore production require a continuous oxygen supply, and they also need to expel some gases that require purification, such as CO2 produced during metabolism, as well as fungal spores. To protect the health of operators and prevent cross-contamination of the laboratory environment, bacteria and mold generated during fermentation are purified through multiple layers of purification using HEPA filter membrane purification plate 340, activated carbon composite purification plate 350, and ceramic filter purification plate 360. During operation, oxygen is supplied to the fungal solid material in the experimental fermentation chamber 100 through the external oxygen supply equipment connected to the pipe 310. To ensure the air pressure in the experimental fermentation chamber 100 and to remove some gases that need purification, such as CO2 produced by metabolism, the gas enters the purification chamber 330 through the connecting pipe 320 and is purified by the HEPA filter membrane purification plate 340, activated carbon composite purification plate 350, and ceramic filter purification plate 360. After purification, the gas is discharged through the exhaust pipe 370.

[0026] In some specific implementation schemes, the pipe body 310 is equipped with a solenoid valve 311, and the connecting pipe 320 is equipped with a second solenoid valve 321.

[0027] In some specific implementation schemes, the experimental fermentation chamber 100 has an opening on one side, a door 120 is hinged to the door 120 on one side, an external support 121 is installed on one side of the door 120, and a heater 110 is provided inside the experimental fermentation chamber 100 to control the temperature of the experimental fermentation chamber 100.

[0028] Working principle: During use, the fungal solid to be fermented is placed in the placement box 280. The placement box 280 is initially connected by the connecting strip 281 and the connecting groove, and then fixed by the plate 282 and bolts 283. The door 120 is then closed. When oxygen supply is needed, the fungal solid material in the experimental fermentation chamber 100 is supplied with oxygen through the pipe 310 connected to an external oxygen supply device. When gas needs to be discharged, the gas enters the purification chamber 330 through the connecting pipe 320 and is purified by the HEPA filter membrane plate 340. The activated carbon composite purification plate 350 and the ceramic filter purification plate 360 ​​perform multi-level and comprehensive purification. At the same time, the motor 220 drives the rotating rod 230, which in turn drives the rotating plate 240 to rotate. This causes the connecting rod 250 to move the moving rod 260. The moving rod 260 moves laterally in conjunction with the experimental fermentation chamber 100, causing the material in the placement box 280 to be turned over by the protruding strip 290 during the lateral movement. This makes the material and gas contact more uniform, ensuring uniform mycelial growth.

[0029] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A biocontrol fungi laboratory small-scale solid fermentation device, characterized in that, The experimental fermentation chamber includes a solid placement mechanism for biocontrol fungi installed on one side and an oxygen supply mechanism installed at one end. The solid placement mechanism for biocontrol fungi includes a mounting plate, which is installed on one side of the experimental fermentation chamber. A motor is installed on one side of the mounting plate, and a rotating rod is rotatably installed on the other side of the mounting plate. The rotating rod is driven by the motor. A rotating plate is installed at one end of the rotating rod. A connecting rod is hinged to a non-central position on one side of the rotating plate. A moving rod is hinged to one end of the connecting rod. The moving rod slides and seals through the experimental fermentation chamber. A moving seat is installed at one end of the moving rod. A placement box is detachably installed on the upper end of the moving seat. A protruding strip is provided inside the placement box.

2. The biocontrol fungi laboratory small-scale solid fermentation device according to claim 1, characterized in that, A connecting strip is installed at the bottom of the electric placement box, and a connecting groove is provided at the upper end of the movable seat in conjunction with the connecting strip. A plate is installed at one end of the connecting strip, and threaded grooves are provided on one side of the plate and the movable seat and are fixed by bolts.

3. The laboratory scale solid state fermentation device for biocontrol fungi according to claim 1, wherein, A guide block is installed at the bottom of the movable seat, and a guide groove is provided at the inner end of the experimental fermentation box. The guide block is slidably connected in the guide groove.

4. A small-scale solid-state fermentation device for biocontrol fungi in a laboratory according to claim 1, characterized in that, A second external support is installed on one side of the placement box, and the protruding strip is set in an arc shape.

5. A small-scale solid-state fermentation device for biocontrol fungi in a laboratory according to claim 1, characterized in that, The oxygen supply mechanism includes a pipe body. One end of the experimental fermentation chamber is connected to the pipe body and a connecting pipe. The experimental fermentation chamber is connected to an external oxygen supply device through the pipe body. A purification chamber is installed at the upper end of the experimental fermentation chamber. The purification chamber and the connecting pipe are connected. The inner wall of the purification chamber is equipped with a HEPA filter membrane purification plate, an activated carbon composite purification plate, and a ceramic filter purification plate. An exhaust pipe is connected to the upper end of the purification chamber.

6. A small-scale solid-state fermentation device for biocontrol fungi in a laboratory according to claim 5, characterized in that, The pipe body is equipped with a solenoid valve, and the connecting pipe is equipped with a second solenoid valve.

7. A small-scale solid-state fermentation device for biocontrol fungi in a laboratory according to claim 1, characterized in that, The experimental fermentation chamber has an opening on one side, and a door is hinged to the side of the experimental fermentation chamber. An external support is installed on one side of the door, and a heater is installed inside the experimental fermentation chamber.

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