Automated plasmid purification device

Abstract

The utility model relates to an automatic plasmid purification device, install big and small draw mould group, inject liquid module group, lift drive module group, translation drive module group to control system, the upper part of big and small draw mould group is big and small draw box, is equipped with negative pressure suction system in big and small draw box, big and small draw box detachably installs plasmid purification board, plasmid purification board is uniformly distributed with several columns of hole, each column has N hole, and each hole is connected with negative pressure suction system, the lower part of big and small draw mould group is equipped with incoming and outgoing drive arrangement, inject liquid module group is equipped with M groups of inject liquid module, and each inject liquid module is independent, and the inside of inject liquid module is equipped with passageway, and the equivalent equal division of reagent solution is N, and is exported through N inject liquid mouth, inject liquid module group installs on lift drive module group, and lift drive module group is installed on translation drive module group with inject liquid module group. The utility model can realize automatic operation, thereby reduce the work load, improve work efficiency.

Description

Technical Field

[0001] This utility model relates to an apparatus for the automatic purification of plasmids in the field of life sciences, and in particular to an operating apparatus that requires the addition of multiple reagents for purification, belonging to the field of life science automation equipment. Background Technology

[0002] In the life sciences, different reagents are often used to purify plasmids. Traditional purification processes require manual operation by operators, followed by reagent separation using centrifuges. However, plasmid purification in the life sciences is now very labor-intensive. If manual operation is still relied upon, a large number of operators are needed, which is labor-intensive and prone to errors.

[0003] Therefore, it is necessary to design an automated plasmid purification device to reduce the labor intensity of operators while improving work efficiency and quality. Utility Model Content

[0004] The purpose of this invention is to provide an automated plasmid purification device that enables automated liquid injection and separation, and to automatically and efficiently complete plasmid purification operations.

[0005] To achieve the above-mentioned utility model objectives, this utility model provides an automatic plasmid purification device, which is equipped with a frame and includes a large and small suction module, a liquid injection module, a lifting drive module, a translation drive module, and a control system.

[0006] The upper part of the large and small suction modules is the large and small suction boxes, which are equipped with a negative pressure suction system. The negative pressure suction system is connected to the external negative pressure pump and waste liquid tank. The upper part of the large and small suction boxes has a mounting base, on which a plasmid purification plate is detachably installed. The plasmid purification plate has several rows of holes evenly distributed, with N holes in each row, and each hole is connected to the negative pressure suction system.

[0007] The lower part of the large and small drawer units is equipped with an inlet / outlet drive device, which is a servo screw drive device. The moving nut of the servo screw drive device is connected to the bottom of the large and small drawer units.

[0008] The injection module has M injection modules, each independent of the others. Each injection module has a liquid inlet, which is connected to an injection pump and a reagent solution cylinder via a pipeline. There are M injection pumps and reagent solution cylinders, where M is greater than or equal to 1. The injection module has internal channels that divide the reagent solution into N equal portions, which are then output through N injection nozzles. The N injection nozzles are evenly distributed at the bottom of the injection module, and the spacing between the injection nozzles is equal to the spacing between the holes in each row of the plasmid purification plate.

[0009] The liquid injection module is installed on the lifting drive module, and the lifting drive module together with the liquid injection module is installed on the translation drive module;

[0010] The lifting drive module is a servo screw drive device. The liquid injection module fixing plate is installed on the moving nut of the servo screw drive device, and the liquid injection module is installed on the liquid injection module fixing plate.

[0011] The translation drive module is a servo screw drive device. The motion nut of the servo screw drive device is equipped with a translation fixing plate, and the lifting drive module is mounted on the translation fixing plate.

[0012] As a further improvement of this utility model, the control system includes a power supply module, a control module, and a drive module;

[0013] The power supply module is connected to the control module and the drive module; the control module is connected to the sensor and the drive module.

[0014] The driver module has three components;

[0015] The servo screw drive devices for the in-and-out drive unit, the lifting drive module, and the translation drive module are each connected to a drive module.

[0016] As a further improvement of this utility model, a cable chain device is provided; one end of the cable chain device is fixed on the frame, and the other end of the cable chain device is connected to the lifting drive module.

[0017] The pipeline connected to the liquid injection module and the drive detection wire connected to the lifting drive module are installed inside the cable chain device.

[0018] As a further improvement of this utility model, all three servo screw drive devices include a screw and a servo motor.

[0019] A motion nut is fitted onto the lead screw, and one end of the lead screw is connected to a servo motor.

[0020] Furthermore, a limit detection device is provided on the side of the servo screw drive device;

[0021] A limit indicator plate is installed on the side of the moving nut;

[0022] Several limit sensors are provided along the axis of the lead screw of the servo lead screw drive device;

[0023] The limit indicator plate is matched with the limit sensor.

[0024] As a further improvement of this utility model, the top four corners of the large and small drawers are provided with limiting corner codes, and the internal space dimensions formed by the limiting corner codes match the external dimensions of the plasmid purification plate.

[0025] Furthermore, positioning sensors are installed on both the large and small sampling boxes, and these sensors are aligned with the plasmid purification plates.

[0026] As a further improvement of this utility model, an anti-crystallization groove is provided outside the large and small drawing modules, and the anti-crystallization groove is located on the translational movement path of the liquid injection module.

[0027] The anti-crystallization tank is equipped with M independent liquid tanks, and the interval between the liquid tanks is the same as the interval between the liquid injection modules of the liquid injection module.

[0028] All the injection nozzles of each injection module in the injection unit can be inserted into the same liquid tank at the same time;

[0029] The injection nozzles of all M injection modules of the injection module can be simultaneously inserted into the liquid tank of the anti-crystallization tank.

[0030] Furthermore, the bottom of the anti-crystallization tank is conical, with inlet and outlet channels at the bottom. Inlet and outlet interfaces are located at the bottom of these channels and are connected to external solution and waste liquid cylinders via pipelines.

[0031] As a further improvement of this utility model, a linear guide device is provided inside the translation drive module;

[0032] The linear guide device includes a linear guide rail that is fixed along the axis of the lead screw, and a linear slider that is mounted on the back of the translation fixed plate. The linear slider is mounted on the linear guide rail.

[0033] The automatic plasmid purification device of this invention has three sets of servo screw drive devices inside, forming an XYZ cross orthogonal coordinate system. Among them, the servo screw drive device of the translation drive module is the X-axis, with the initial position at point 0, and the translation towards the plasmid purification plate is the positive direction; the servo screw drive device of the large and small extraction module is the Y-axis, with the internal position at point 0, and the outward output is the positive direction; the servo screw drive device of the lifting drive module is the Z-axis, with the initial position at point 0, and the downward direction is the positive direction.

[0034] When the automatic plasmid purification device of this invention is working, the servo screw drive of the large and small extraction modules moves to the waiting position in the positive Y-axis direction, places the plasmid purification plate on it, and then the infeed servo motor starts, driving the plasmid purification plate to the zero position of the Y-axis, waiting for the operation to begin; the M groups of injection modules of the injection module are respectively connected to the injection pumps and reagent solution cylinders of the different reagents needed; when the automatic plasmid extraction program is started, the servo screw drive of the translation drive module starts, moving the injection module along the positive X-axis direction to above the plasmid purification plate, so that the injection module containing the first reagent in the injection module is aligned with the starting row of holes on the plasmid purification plate; then, as needed, the servo screw drive of the lifting drive module starts, moving the injection module along the positive Z-axis direction, and aligning the... The injection nozzle is lowered to a suitable height directly above the well to be injected. The injection pump connected to the injection module for the first reagent is then activated to precisely inject the reagent into one row of wells on the plasmid purification plate. After one row of wells is injected, the lifting drive module moves the injection module along the negative Z-axis, raising the injection nozzle appropriately. Then, the servo screw drive of the translation drive module moves precisely a short distance, positioning the injection nozzle directly above the next row of wells to be injected. The servo screw drive of the lifting drive module is then activated again to lower the injection nozzle to a suitable height. The injection pump connected to the injection module is then activated again to precisely inject the reagent into the next row of wells on the plasmid purification plate. This cycle continues until the first reagent is injected.

[0035] After the first reagent injection is completed, the servo screw drive of the translation drive module reverses its direction, moving the injection module to the anti-crystallization tank. Then, the servo screw drive of the lifting drive module is activated to lower the injection module, allowing the injection nozzles to be inserted into their respective anti-crystallization tanks to prevent reagent crystallization. During this process, the plasmid purification plate begins to react after reagent injection. A reaction waiting time is set, and after the time is up, the negative pressure suction system in the large and small suction modules is activated. According to business needs, the negative pressure working time is set to aspirate the reagent from the plasmid purification plate.

[0036] After the first reagent reaction is complete and the residual liquid has been aspirated, the lifting and translating drive modules are restarted to move the injection nozzles of the next injection module to the starting cavity of the plasmid purification plate. Once the injection nozzles are at the correct height from the cavities, the injection pump of the second reagent injection module (connected to the next injection module) is activated to continue precise injection into the cavities of the plasmid purification plate. This process continues until the second reagent injection is complete. Then, the lifting and translating drive modules return the injection modules to the anti-crystallization tank position, and the injection nozzles are inserted back into their respective anti-crystallization tanks. After the second reagent reaction is complete in the plasmid purification plate, the negative pressure suction system in the large and small suction modules is activated to aspirate the residual liquid from the second reagent reaction in the plasmid purification plate. After the residual liquid from the second reagent reaction is aspirated, the injection, reaction, and aspiration operations for a third reagent, or even more reagents, can be performed as needed.

[0037] Once everything is complete, the operator can remove the plasmid purification plate, replace it with a new one, and continue the operation. Alternatively, an automated robotic arm can be installed in the automatic plasmid purification device of this invention to automatically pick up and replace the plasmid purification plate, further automating the plasmid purification process.

[0038] This invention relates to an automated plasmid purification device, which enables automated operation, thereby reducing workload. One operator can simultaneously monitor multiple devices, improving work efficiency. The automated liquid injection system eliminates the need for manual operation, resulting in more precise and consistent injection and eliminating potential errors or mistakes caused by manual operation. The device can also perform automated aspiration operations, allowing for more precise time control and improved product quality. Furthermore, during operation, operators can remain away from reagents, eliminating the health and safety hazards posed by the volatilization of irritating reagents. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the overall structural effect of the automatic plasmid purification device of this utility model;

[0040] Figure 2 This is a schematic diagram of the internal structure of the automatic plasmid purification device of this utility model;

[0041] Figure 3 This is a schematic diagram of the control system structure of the automatic plasmid purification device of this utility model;

[0042] Figure 4 This is a schematic diagram of the overall structure of the large and small drawing modules;

[0043] Figure 5 This is a schematic diagram of the plasmid purification plate installation.

[0044] Figure 6 This is a schematic diagram of the overall structure of the injection system;

[0045] Figure 7 A schematic diagram of the internal structure of the crystallization tank;

[0046] Figure 8 A cross-sectional view of the crystallization tank;

[0047] Figure 9 This is a schematic diagram of the overall structure of the liquid injection module;

[0048] Figure 10 This is a schematic diagram of the overall structure of the liquid injection module;

[0049] Figure 11 This is a schematic diagram of the overall structure of the lifting drive module;

[0050] Figure 12 This is a schematic diagram of the overall structure of the translation drive module;

[0051] Figure 13 This is a schematic diagram of the overall structure of the translation drive module. Detailed Implementation

[0052] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings.

[0053] The automatic plasmid purification device of this invention has the following overall structure: Figure 1 As shown, each component is installed inside or on the frame 1. The internal structure after opening the protective housing is as follows: Figure 2 , Figure 3 As shown, it mainly includes a plasmid purification plate 2, a large and small extraction module 3, an anti-crystallization tank 4, a liquid injection module 5, a lifting drive module 6, and a translation drive module 7. In order to achieve reliable large-range translational movement, a drag chain device 8 is also installed on the lifting drive module 6.

[0054] The rear of the rack 1 is equipped with a control system, including a power supply module 12, a control module 13, and a drive module 14. The power supply module 12 provides centralized voltage transformation, stabilization, and power supply; the control module 13 performs detection and control; and the drive module 14 provides precise drive control for the drive motors in the system. The modular design facilitates system construction and maintenance.

[0055] Plasmid purification plate 2 is set on the large and small extraction module group 3, and the specific structure is as follows: Figure 4 , Figure 5 As shown, plasmid purification plate 2 is generally a standard plate. In this embodiment, an 8×12 96-well plate is used, which is connected to the negative pressure suction system in the large and small suction module group 3.

[0056] The bottom of the large and small drawer module 3 is the inlet / outlet drive device, which is a servo screw drive device, including an inlet / outlet screw 33 and an inlet / outlet servo motor 34. An inlet / outlet nut 32 is sleeved on the inlet / outlet screw 33, and the large and small drawer boxes 31 are installed above the inlet / outlet nut 32. One end of the inlet / outlet screw 33 is connected to the inlet / outlet servo motor 34. The inlet / outlet servo motor 34 is controlled by the drive module 14 to rotate, thereby driving the inlet / outlet screw 33 to rotate. Finally, the rotation is converted into linear motion through the inlet / outlet nut 32, which drives the large and small drawer boxes 31 and the plasmid purification plate 2 to achieve inlet / outlet movement.

[0057] To prevent the servo screw drive from moving beyond its range and affecting equipment safety, a limit detection device is also installed. This includes an entry / exit limit indicator plate 35 installed at the bottom of the large and small drawers 31, and entry / exit limit sensors 36, typically infrared sensors, installed at both ends along the axis of the entry / exit screw 33 of the servo screw drive. These sensors work in conjunction with the entry / exit limit indicator plate 35. When the entry / exit limit indicator plate 35 moves with the large and small drawers 31 to the entry / exit limit sensor 36 at one end, it blocks the light from the infrared sensor, indicating that the movement has reached the limit position and the entry / exit servo motor 34 needs to be stopped.

[0058] The large and small drawer boxes 31 are equipped with a negative pressure suction system, which is connected to an external negative pressure suction pump and waste liquid tank through an interface.

[0059] Limiting corner brackets 38 are provided at the top four corners of the large and small drawer boxes 31. The internal space dimensions formed by the limiting corner brackets 38 match the external dimensions of the plasmid purification plate 2, thereby facilitating the rapid positioning and installation of the plasmid purification plate 2 on the large and small drawer boxes 31.

[0060] Furthermore, the large and small sampling boxes 31 are also equipped with installation position sensors 37 to detect the plasmid purification plate 2. When the plasmid purification plate 2 is installed in place, the installation position sensor 37 will send a signal indicating that it is in place. Otherwise, if the installation position sensor 37 sends a signal indicating that it is not in place, subsequent operations cannot be started.

[0061] The injection module 5 is mounted on the cross-drive module, which includes a lifting drive module 6 and a translation drive module 7. An anti-crystallization tank 4 is also provided near the initial position. Figure 6 As shown.

[0062] As needed, the liquid injection module 5 of this utility model is provided with three sets of independent liquid injection modules, and the corresponding anti-crystallization tank 4 is provided with three independent liquid tanks 41, such as... Figure 7 , Figure 8 As shown; the bottom of the liquid tank 41 is a conical bottom 42, which makes it easy to drain the solution in the liquid tank 41. Furthermore, an inlet / outlet channel 43 is provided at the bottom of the conical bottom 42 of the liquid tank 41, and an inlet / outlet interface 44 is provided on the outside of the bottom to facilitate connection with external solution cylinders and waste liquid cylinders.

[0063] The specific structure of the liquid injection module 5 is as follows: Figure 9 , Figure 10 As shown, three sets of injection modules are provided. Each injection module has an inlet port 52, which is connected to the injection pump and solution cylinder via a pipeline. The injection module has internal channels, and based on the cavity design of the plasmid purification plate 2, eight parallel injection nozzles 53 are provided, corresponding to a row of eight cavities on the plasmid purification plate 2. This means that a single injection module can simultaneously inject liquid into a row of eight cavities on the plasmid purification plate 2. Due to the uniform and equal-volume design within the injection module, equal-volume injection from the eight injection nozzles 53 is achieved, thus ensuring equal-volume injection into the row of eight cavities on the plasmid purification plate 2. The three sets of injection modules are arranged side-by-side, forming an 8×3 array of injection nozzles 53 at the bottom of the injection module 5.

[0064] The injection module 5 is fixedly mounted on the injection module mounting plate 61 of the lifting drive module 6; the specific structure of the lifting drive module 6 is as follows: Figure 11 As shown, this is also a servo screw drive device, including a lifting screw 62 and a lifting servo motor 64. A lifting nut 63 is sleeved on the lifting screw 62, and the liquid injection module fixing plate 61 is fixedly installed on the lifting nut 63, thereby fixing the liquid injection module 5. One end of the lifting screw 62 is connected to the lifting servo motor 64. The lifting servo motor 64 is controlled by the drive module 14 to rotate, thereby driving the lifting screw 62 to rotate. Finally, the rotation is converted into linear motion through the lifting nut 63, which drives the liquid injection module fixing plate 61 and the liquid injection module 5 on it to achieve lifting and lowering motion.

[0065] To prevent the servo screw drive from moving beyond its range, a limit detection device is also installed. This includes a lifting limit indicator plate 67 installed on the side of the lifting nut 63, and lifting limit sensors 68 installed along the axis of the lifting screw 62 of the servo screw drive to limit the lifting height range of the injection module 5. The lifting limit sensors 68 are generally infrared sensors, which work in conjunction with the lifting limit indicator plate 67. When the lifting limit indicator plate 67 moves with the lifting nut 63 and the injection module 5 to the lifting limit sensor 68 at one end in the height direction, it blocks the light from the infrared sensor, indicating that the movement has reached the limit position or the specified height position. At this time, the lifting servo motor 64 needs to be stopped to prevent the equipment from being bumped or damaged.

[0066] The lifting drive module 6 has a lifting base plate 60 on the back, which allows the servo screw drive device and the limit detection device to be installed together to form a module, making it easy to disassemble and replace.

[0067] The lifting drive module 6, together with the liquid injection module 5, is fixedly mounted on the translation fixing plate 71 of the translation drive module 7; that is, the lifting base plate 60 is directly fixedly mounted on the translation fixing plate 71. The specific structure of the translation drive module 7 is as follows: Figure 12 , Figure 13 As shown, this is also a servo screw drive device, including a translation screw 72 and a translation servo motor 74. A translation nut 73 is sleeved on the translation screw 72, and the translation fixing plate 71 is fixedly installed on the translation nut 73, thereby fixing the lifting drive module 6. One end of the translation screw 72 is connected to the translation servo motor 74. The translation servo motor 74 is controlled by the drive module 14 to rotate, thereby driving the translation screw 72 to rotate, and finally converting it into linear motion through the translation nut 73, driving the lifting drive module 6 and the liquid injection module 5 to achieve translational motion.

[0068] Because the lifting drive module 6 and the liquid injection module 5 are relatively heavy, in order to improve the reliability of the translational movement, a linear guide device is also provided along the axial direction of the translation screw 72. This includes a linear guide rail 75 that is mounted and fixed on the base plate along the axial direction of the translation screw, and a linear slider 76 that is mounted on the back of the translation fixed plate 71. The linear slider 76 is mounted on the linear guide rail 75 to form a set of linear guide devices. More preferably, linear guide devices are provided at both the top and bottom of the translation screw 72, so as to reliably support the translation fixed plate 71 and guide its linear movement.

[0069] The translation drive module 7 is also equipped with a limit detection device, including a translation limit indicator plate 77 installed on the side of the translation nut 73 or the translation fixing plate 71, and a translation limit sensor 78 is provided along the axis of the translation screw 72 of the servo screw drive device to limit or detect the translation range or points on the translation movement of the liquid injection module 5; that is, as Figure 13 As shown, a set of translation limit sensors 78 is provided at the top to detect the limit of the liquid injection module 5 moving to the end position of the plasmid purification plate 2, and a set of translation limit sensors 78 is also provided at the bottom to detect the positioning of the liquid injection module 5 moving to the position of the anti-crystallization tank 4.

[0070] The automatic plasmid purification device of this invention is equipped with three sets of servo screw drive devices, such as... Figure 2 As shown, an orthogonal XYZ coordinate system is formed; among them, the servo screw drive device of the translation drive module 7 is the X-axis, with the initial position at point 0, and the translation towards the plasmid purification plate 2 is the positive direction; the servo screw drive device of the large and small extraction module 3 is the Y-axis, with the internal position at point 0, and the output direction is the positive direction; the servo screw drive device of the lifting drive module 6 is the Z-axis, with the initial position at point 0, and the downward direction is the positive direction.

[0071] When the automatic plasmid purification device of this utility model is working, the servo screw drive of the large and small extraction module 3 moves to the waiting position in the positive direction of the Y-axis, places the plasmid purification plate 2 on it, and then the infeed servo motor 34 starts, driving the plasmid purification plate 2 to the zero position of the Y-axis, waiting for the work to begin; the three injection modules of the injection module 5 are respectively connected to the injection pump and reagent solution cylinder of the different reagents to be used; when the automatic plasmid extraction program is started, the servo screw drive of the translation drive module 7 starts, moving the injection module 5 along the positive direction of the X-axis, moving it above the plasmid purification plate 2, so that the injection module of the first reagent in the injection module 5 is aligned with the starting row hole on the plasmid purification plate 2; then, as needed, the servo screw drive of the lifting drive module 6 starts, moving the injection module 5 along the positive direction of the Z-axis, and placing the injection nozzle 5 ready for injection. 3. Lower the nozzle to a suitable height directly above the well to be injected. Start the injection pump connected to the injection module of the first reagent in injection module 5 to precisely inject the first reagent into one row of wells on plasmid purification plate 2 for plasmid purification reaction. After one row of wells is injected, the lifting drive module 6 drives the injection module 5 to move along the negative Z-axis, raising the injection nozzle 53 appropriately. Then, the servo screw drive device of the translation drive module 7 moves precisely a distance again, so that the injection nozzle 53 of the injection module 5 is positioned directly above the next row of wells to be injected. Then, the servo screw drive device of the lifting drive module 6 is started again to lower the injection nozzle 53 to a suitable height. The injection pump connected to the injection module of injection module 5 is started again to precisely inject the first reagent into the next row of wells on plasmid purification plate 2. This cycle continues until the first reagent is injected.

[0072] After the first reagent injection is completed, the servo screw drive of the translation drive module 7 reverses its direction, moving the injection module 5 to the anti-crystallization tank 4. Then, the servo screw drive of the lifting drive module 6 is activated to lower the injection module 5, allowing the injection nozzles 53 to be inserted into their respective anti-crystallization tanks 41, preventing reagent crystallization within the injection nozzles 53. During this process, the plasmid purification plate begins to react after the first reagent is injected. A reaction waiting time is set, and after the time is up, the negative pressure suction system in the large and small suction modules 3 is activated. Based on business needs, the negative pressure working time is set to suction out the reagent in the plasmid purification plate 2.

[0073] After the first reagent reaction is complete and the residual liquid after the reaction has been absorbed, the lifting drive module 6 and the translation drive module 7 are restarted to move the injection nozzle 53 of the next injection module of the injection module 5 to the starting row of holes on the plasmid purification plate 2. After the injection nozzle 53 is at the appropriate height with the hole, the injection pump of the second reagent injection module is started to continue to accurately inject liquid into the holes of the plasmid purification plate 2. This continues until the second reagent injection is completed. Then, the lifting drive module 6 and the translation drive module 7 drive the injection module 5 back to the anti-crystallization tank 4 position and insert the injection nozzle 53 into the liquid tank 41 of its respective anti-crystallization tank 4. At the same time, wait for the second reagent to complete the reaction in the plasmid purification plate 2, and then start the negative pressure suction system in the large and small suction modules 3 to absorb the residual liquid of the second reagent reaction in the plasmid purification plate 2.

[0074] After the residual liquid from the second reagent reaction is completely absorbed, the third reagent can be added, reacted, and absorbed again as needed.

[0075] Once everything is complete, the operator can remove plasmid purification plate 2, replace it with a new plasmid purification plate 2, and continue the operation. Alternatively, an automatic robotic arm can be installed in the automatic plasmid purification device of this invention to automatically pick up and replace the plasmid purification plate 2, further automating the plasmid purification process.

[0076] In the embodiment of the automatic plasmid purification device of this utility model, the liquid injection module 5 is equipped with three sets of independently operating liquid injection modules, and the corresponding anti-crystallization tank 4 is also equipped with three independent liquid tanks 41, so as to meet the automated liquid injection operation of three different reagents; of course, more sets of parallel liquid injection modules and liquid tanks 41 can also be set as needed, such as four sets, five sets, etc., so as to meet the needs of more complex plasmid purification operations.

[0077] This invention relates to an automated plasmid purification device, which enables automated operation, thereby reducing workload. One operator can simultaneously monitor multiple devices, improving work efficiency. The automated liquid injection system eliminates the need for manual operation, resulting in more precise and consistent injection and eliminating potential errors or mistakes caused by manual operation. The device can also perform automated aspiration operations, allowing for more precise time control and improved product quality. Furthermore, during operation, operators can remain away from reagents, eliminating the health and safety hazards posed by the volatilization of irritating reagents.

[0078] The preferred embodiments of this utility model have been described in detail above, but this utility model is not limited to the embodiments described. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of this utility model, and these equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. An automated plasmid purification device, characterized in that, It is equipped with a frame, which houses large and small suction modules, liquid injection modules, lifting drive modules, translation drive modules, and a control system; The upper part of the large and small suction modules is the large and small suction boxes, which are equipped with a negative pressure suction system. The negative pressure suction system is connected to the external negative pressure pump and waste liquid tank. The upper part of the large and small suction boxes has a mounting base, on which a plasmid purification plate is detachably installed. The plasmid purification plate has several rows of holes evenly distributed, with N holes in each row, and each hole is connected to the negative pressure suction system. The lower part of the large and small drawer units is equipped with an inlet / outlet drive device, which is a servo screw drive device. The moving nut of the servo screw drive device is connected to the bottom of the large and small drawer units. The injection module has M injection modules, each independent of the others. Each injection module has a liquid inlet, which is connected to an injection pump and a reagent solution cylinder via a pipeline. There are M injection pumps and reagent solution cylinders, where M is greater than or equal to 1. The injection module has internal channels that divide the reagent solution into N equal portions, which are then output through N injection nozzles. The N injection nozzles are evenly distributed at the bottom of the injection module, and the spacing between the injection nozzles is equal to the spacing between the holes in each row of the plasmid purification plate. The liquid injection module is installed on the lifting drive module, and the lifting drive module together with the liquid injection module is installed on the translation drive module; The lifting drive module is a servo screw drive device. The liquid injection module fixing plate is installed on the moving nut of the servo screw drive device, and the liquid injection module is installed on the liquid injection module fixing plate. The translation drive module is a servo screw drive device. The motion nut of the servo screw drive device is equipped with a translation fixing plate, and the lifting drive module is mounted on the translation fixing plate.

2. The automated plasmid purification apparatus of claim 1, wherein, The control system includes a power module, a control module, and a drive module; The power supply module is connected to the control module and the drive module; the control module is connected to the sensor and the drive module. The driver module has three components; The servo screw drive devices for the in-and-out drive unit, the lifting drive module, and the translation drive module are each connected to a drive module.

3. The automated plasmid purification apparatus of claim 1, wherein It is equipped with a cable chain device; one end of the cable chain device is fixed on the frame, and the other end of the cable chain device is connected to the lifting drive module. The pipeline connected to the liquid injection module and the drive detection wire connected to the lifting drive module are installed inside the cable chain device.

4. The automated plasmid purification apparatus of claim 1, wherein All three servo screw drive devices include a screw and a servo motor; A motion nut is fitted onto the lead screw, and one end of the lead screw is connected to a servo motor.

5. The automated plasmid purification apparatus of claim 4, wherein The servo screw drive is equipped with a limit detection device. A limit indicator plate is installed on the side of the moving nut; Several limit sensors are provided along the axis of the lead screw of the servo lead screw drive device; The limit indicator plate is matched with the limit sensor.

6. The automated plasmid purification apparatus of claim 1, wherein The top four corners of the large and small boxes are equipped with limiting corner brackets, and the internal space dimensions formed by the limiting corner brackets match the external dimensions of the plasmid purification plate.

7. The automated plasmid purification apparatus of claim 1 or 6, wherein The large and small boxes are equipped with positioning sensors, which are aligned with the plasmid purification plates.

8. The automated plasmid purification apparatus of claim 1, wherein, An anti-crystallization groove is provided outside the large and small drawing modules, and the anti-crystallization groove is located on the translational movement path of the liquid injection module. The anti-crystallization tank is equipped with M independent liquid tanks, and the interval between the liquid tanks is the same as the interval between the liquid injection modules of the liquid injection module. All the injection nozzles of each injection module in the injection unit can be inserted into the same liquid tank at the same time; The injection nozzles of all M injection modules of the injection module can be simultaneously inserted into the liquid tank of the anti-crystallization tank.

9. The automated plasmid purification apparatus of claim 8, wherein, The bottom of the anti-crystallization tank is conical, and the bottom of the conical bottom is provided with inlet and outlet channels. The bottom of the inlet and outlet channels is provided with inlet and outlet interfaces, which are connected to the external solution cylinder and waste liquid cylinder through pipelines.

10. The automated plasmid purification apparatus of claim 1, wherein, The translation drive module is equipped with a linear guide device; The linear guide device includes a linear guide rail that is fixed along the axis of the lead screw, and a linear slider that is mounted on the back of the translation fixed plate. The linear slider is mounted on the linear guide rail.

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