A breast milk oligosaccharide fermenter

Abstract

This utility model relates to the field of bio-fermentation equipment technology, and discloses a human milk oligosaccharide fermentation tank, including a fermentation tank with several support legs fixedly connected to the bottom end of the fermentation tank and a feed valve pipe fixedly connected to the bottom end of the fermentation tank. A top cover is installed on the top end of the fermentation tank, and a feed pipe is fixedly connected to the top end of the top cover. A rotating component is provided on the top end of the top cover, and a dispersing component is provided between the top end and the bottom end of the top cover. A stirring component is provided on the side end of the dispersing component. This utility model allows the dispersing component to rotate through the rotating component. After the feed liquid enters the fermentation tank from the dispersing component, it can be evenly dispersed in the fermentation tank and mixed evenly with other fermentation liquids and feed liquids, avoiding local accumulation of feed liquids after they are poured in all at once, improving the mixing effect and avoiding affecting the uniformity of subsequent fermentation. The setting of the stirring component facilitates the even mixing of fermentation liquids and feed liquids, further improving the mixing effect of fermentation liquids and feed liquids required for human milk oligosaccharide fermentation.

Description

Technical Field

[0001] This utility model relates to the field of biological fermentation equipment technology, and in particular to a human milk oligosaccharide fermentation tank. Background Technology

[0002] Human milk oligosaccharides (HMOs) are a class of complex carbohydrates naturally found in breast milk, playing important physiological roles such as regulating infant gut microbiota and enhancing immunity. Due to the high cost and limited yield of naturally extracted HMOs, HMO fermenters are specialized bioreactors designed to synthesize HMOs through microbial fermentation technology. By simulating the growth and metabolic environment of microorganisms and controlling key parameters such as temperature, pH, oxygen content, and nutrient supply during fermentation, engineered bacteria (such as recombinant E. coli and yeast) can efficiently synthesize target HMOs, ultimately achieving industrial-scale production.

[0003] Existing fermenters use a top-feed single-point system. High-concentration liquids tend to accumulate locally due to gravity and other factors. Conventional stirring devices cannot quickly eliminate the concentration gradient, resulting in uneven cell metabolism and affecting the uniformity of subsequent fermentation. Therefore, a human milk oligosaccharide fermenter is needed to solve these problems. Utility Model Content

[0004] The purpose of this invention is to provide a human milk oligosaccharide fermentation tank, which solves the problem in the prior art where, during the use of a single-point top feeding tank, high-concentration liquids accumulate locally due to gravity and other factors, and conventional stirring devices cannot quickly eliminate the concentration gradient, resulting in uneven cell metabolism and affecting subsequent fermentation.

[0005] This utility model provides the following technical solution: a human milk oligosaccharide fermentation tank, comprising a fermentation tank, a plurality of support legs fixedly connected to the bottom end of the fermentation tank, a feed valve pipe fixedly connected to the bottom end of the fermentation tank, a top cover installed on the top end of the fermentation tank, a feed pipe fixedly connected to the top end of the top cover, a rotating component provided on the top end of the top cover, a dispersing component provided between the top end and the bottom end of the top cover, and a stirring component provided on the side end of the dispersing component.

[0006] As a preferred embodiment of the above technical solution, two round rods are fixedly connected to the top of the top cover, and a fixing plate is fixedly connected between the top ends of the two round rods.

[0007] As a preferred embodiment of the above technical solution, the rotating assembly includes a motor, which is fixedly mounted on the bottom end of the fixed plate. A rotating rod is installed at the output end of the motor, and the bottom end of the rotating rod is rotatably mounted on the top end of the top cover. A gear is fixedly connected to the outer end of the rotating rod.

[0008] As a preferred embodiment of the above technical solution, the dispersing component includes a connecting pipe, which is rotatably disposed between the top end of the top cover and the top end of the fixing plate. Several through holes are opened on the side end of the connecting pipe, and a gear two is fixedly connected to the outer end of the connecting pipe. The gear one meshes with the side end of the gear two, and a sealed bearing is installed at the top end of the connecting pipe.

[0009] As a preferred embodiment of the above technical solution, the stirring assembly includes several connecting plates, each of which is fixedly connected to the side end of a connecting pipe. A scraper is fixedly connected between the side ends of two adjacent connecting plates, and several stirring plates are fixedly connected to the side end of the scraper.

[0010] As a preferred embodiment of the above technical solution, the scraper is sloped at the side end, and each of the stirring plates has several dispersion holes at the side end.

[0011] As a preferred embodiment of the above technical solution, a fixing rod is fixedly connected to the bottom end of the connecting pipe, and several inclined plates are fixedly connected to the side end of the fixing rod.

[0012] Compared with the prior art, the beneficial effects of this utility model are: This invention uses a rotating component to drive the dispersing component to rotate. After the feed liquid enters the fermentation tank from the dispersing component, it can be evenly dispersed in the fermentation tank and mixed evenly with other fermentation liquids and feed liquids. This avoids the local accumulation of feed liquids after they are poured in all at once, which would form a concentration gradient, thus improving the mixing effect and avoiding affecting the uniformity of subsequent fermentation. The stirring component facilitates the even mixing of fermentation liquids and feed liquids, further improving the mixing effect required for the fermentation of human milk oligosaccharides. Attached Figure Description

[0013] Figure 1 A schematic diagram of the three-dimensional structure of a human milk oligosaccharide fermentation tank. Figure 1 ; Figure 2 A three-dimensional structural diagram of the rotating component of a human milk oligosaccharide fermentation tank; Figure 3 A bottom view of a human milk oligosaccharide fermentation tank; Figure 4 This is a schematic diagram of the overall structure of a human milk oligosaccharide fermentation tank.

[0014] In the diagram: 1. Fermentation tank; 101. Support leg; 102. Feed valve pipe; 2. Top cover; 201. Feed pipe; 202. Round rod; 203. Fixing plate; 3. Rotating assembly; 301. Motor; 302. Rotating rod; 303. Gear one; 4. Dispersion assembly; 401. Connecting pipe; 402. Through hole; 403. Gear two; 404. Sealed bearing; 5. Stirring assembly; 501. Connecting plate; 502. Scraper; 503. Stirring plate; 504. Dispersion hole; 6. Fixing rod; 601. Inclined plate. Detailed Implementation

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0016] like Figures 1-4 As shown, this utility model provides a technical solution: a human milk oligosaccharide fermentation tank, including a fermentation tank 1, a plurality of support legs 101 fixedly connected to the bottom end of the fermentation tank 1, a feed valve pipe 102 fixedly connected to the bottom end of the fermentation tank 1, a top cover 2 installed on the top end of the fermentation tank 1, a feed pipe 201 fixedly connected to the top end of the top cover 2, two round rods 202 fixedly connected to the top end of the top cover 2, a fixing plate 203 fixedly connected between the top ends of the two round rods 202, a rotating assembly 3 provided at the top end of the top cover 2, and a distribution plate 3 between the top end and the bottom end of the top cover 2. The dispersing component 4 has a stirring component 5 on its side. The dispersing component 4 can be rotated by rotating the component 3. After the feed liquid enters the fermentation tank 1 from the dispersing component 4, it can be evenly dispersed in the fermentation tank 1 and mixed evenly with other fermentation liquids and feed liquids. This avoids the feed liquid from being poured in all at once and accumulating in one place, thus improving the mixing effect and avoiding affecting the uniformity of subsequent fermentation. The stirring component 5 facilitates the even mixing of the fermentation liquid and feed liquid, further improving the mixing effect of the fermentation liquid and feed liquid required for the fermentation of human milk oligosaccharides.

[0017] As one implementation method in this embodiment, such as Figure 1 , Figure 2 and Figure 3As shown, the rotating assembly 3 includes a motor 301, which is fixedly mounted on the bottom end of the fixed plate 203. A rotating rod 302 is mounted on the output end of the motor 301. The bottom end of the rotating rod 302 is rotatably mounted on the top end of the top cover 2. A gear 303 is fixedly connected to the outer end of the rotating rod 302. The dispersing assembly 4 includes a connecting pipe 401, which is rotatably mounted between the top end of the top cover 2 and the top end of the fixed plate 203. Several through holes 402 are opened on the side end of the connecting pipe 401. A gear 403 is fixedly connected to the outer end of the connecting pipe 401. The gear 303 meshes with the side end of the gear 403. A sealed bearing is mounted on the top end of the connecting pipe 401. 404, the function of the sealed bearing 404 is to facilitate the connection between the connecting pipe 401 and the external pipe without affecting the normal rotation of the connecting pipe 401. In practice, when the feed liquid enters the connecting pipe 401 through the external pipe, the starting motor 301 drives the rotating rod 302 and gear 1 303 to rotate. At this time, gear 1 303 meshes with gear 2 403 to rotate and drive the connecting pipe 401 to rotate. At this time, the feed liquid can be evenly dispersed during the rotation of the connecting pipe 401 and mixed evenly with the other fermentation liquid and feed liquid in the fermenter 1. This avoids the local accumulation of feed liquid after it is poured in all at once, forming a concentration gradient, improving the mixing effect, and avoiding affecting the uniformity of subsequent fermentation.

[0018] As one implementation method in this embodiment, such as Figure 1 , Figure 2 and Figure 3 As shown, the stirring assembly 5 includes several connecting plates 501, all of which are fixedly connected to the side ends of the connecting pipe 401. A scraper 502 is fixedly connected between the side ends of two adjacent connecting plates 501. Several stirring plates 503 are fixedly connected to the side ends of the scraper 502. The side ends of the scraper 502 are sloped. Several dispersion holes 504 are provided on the side ends of each stirring plate 503. A fixing rod 6 is fixedly connected to the bottom end of the connecting pipe 401. Several inclined plates 601 are fixedly connected to the side ends of the fixing rod 6. During the process, after the connecting plate 501, scraper 502, stirring plate 503 and inclined plate 601 rotate, the scraper 502 scrapes the inner wall of the fermentation tank 1, which can prevent unmixed fermentation liquid and feed liquid from adhering to the inner wall. The stirring plate 503 can stir and mix the fermentation liquid and feed liquid. The dispersion hole 504 disperses the stress of the feed liquid and improves the mixing effect. The inclined plate 601 can move the fermentation liquid and feed liquid during the rotation, further improving the mixing effect of the fermentation liquid and feed liquid required for the fermentation of human milk oligosaccharides.

[0019] Working principle: When the feed liquid enters the connecting pipe 401 through the external pipe, the starting motor 301 drives the rotating rod 302 and gear 303 to rotate. At this time, gear 303 meshes with gear 403, causing the connecting pipe 401, connecting plate 501, scraper 502, stirring plate 503, and inclined plate 601 to rotate. During the rotation of the connecting pipe 401, the feed liquid is evenly dispersed and mixed with other fermentation liquids and feed liquids in fermenter 1. Meanwhile, scraper 502 scrapes against the inner wall of fermenter 1. This design prevents unmixed fermentation broth and feed liquid from adhering to the inner wall. The stirring plate 503 can stir and mix the fermentation broth and feed liquid, while the dispersion hole 504 disperses the stress in the feed liquid, improving the mixing effect. The inclined plate 601 can move the fermentation broth and feed liquid during rotation, further improving the mixing effect of the fermentation broth and feed liquid required for the fermentation of human milk oligosaccharides. This prevents the feed liquid from piling up after being poured in all at once, which would cause local accumulation and form a concentration gradient, thus improving the mixing effect and avoiding affecting the uniformity of subsequent fermentation.

[0020] The above embodiments are only used to illustrate the technical solution of this utility model, and are not intended to limit it.

Claims

1. A human milk oligosaccharide fermentation tank, comprising a fermentation tank (1), wherein a plurality of support legs (101) are fixedly connected to the bottom end of the fermentation tank (1), a discharge valve pipe (102) is fixedly connected to the bottom end of the fermentation tank (1), and a top cover (2) is installed on the top end of the fermentation tank (1), wherein a feed pipe (201) is fixedly connected to the top end of the top cover (2), characterized in that: The top of the top cover (2) is provided with a rotating component (3), and a dispersing component (4) is provided between the top and bottom of the top cover (2). A stirring component (5) is provided on the side of the dispersing component (4).

2. The human milk oligosaccharide fermenter of claim 1, wherein: The top of the top cover (2) is fixedly connected to two round rods (202), and a fixing plate (203) is fixedly connected between the top ends of the two round rods (202).

3. The human milk oligosaccharide fermenter of claim 2, wherein: The rotating assembly (3) includes a motor (301), which is fixedly installed at the bottom of the fixed plate (203). A rotating rod (302) is installed at the output end of the motor (301). The bottom end of the rotating rod (302) is rotatably set at the top of the top cover (2). A gear (303) is fixedly connected to the outer end of the rotating rod (302).

4. The human milk oligosaccharide fermenter of claim 3, wherein: The dispersion component (4) includes a connecting pipe (401), which is rotatably disposed between the top end of the top cover (2) and the top end of the fixing plate (203). The side end of the connecting pipe (401) is provided with several through holes (402). The outer end of the connecting pipe (401) is fixedly connected to a gear two (403). The gear one (303) meshes with the side end of the gear two (403). A sealed bearing (404) is installed at the top end of the connecting pipe (401).

5. The human milk oligosaccharide fermentation tank of claim 4, wherein: The stirring assembly (5) includes several connecting plates (501), which are fixedly connected to the side end of the connecting pipe (401). A scraper (502) is fixedly connected between the side ends of two adjacent connecting plates (501), and several stirring plates (503) are fixedly connected to the side end of the scraper (502).

6. The human milk oligosaccharide fermentation tank of claim 5, wherein: The scraper (502) has a sloping side, and each of the stirring plates (503) has several dispersion holes (504) on its side.

7. The human milk oligosaccharide fermentation tank of claim 6, wherein: The bottom end of the connecting pipe (401) is fixedly connected to a fixing rod (6), and the side end of the fixing rod (6) is fixedly connected to several inclined plates (601).

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