Intelligent concentrating equipment for stem cell exosome

Abstract

This utility model discloses an intelligent concentration device for stem cell exosomes, belonging to the field of exosome filtration technology. It includes a frame, with an equipment box installed inside the frame. A linear module is located on the front side of the equipment box, and a pumping device is installed on one side of the linear module's movable seat. In this utility model, the pumping device can be laterally displaced via the rear linear module, which facilitates adapting to the arrangement of multiple tanks on the front side. Furthermore, the peristaltic pump can meet the peristaltic pumping needs at different lateral positions, facilitating the processing of samples of different volumes and concentrations, improving media transport and replacement efficiency. The buffer component ensures that the supply pipe remains partially bent when connected to tanks at multiple lateral positions, maintaining media transport stability and avoiding the impact of the coiled and bent supply pipes in traditional technologies on concentration and filtration efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of exosome filtration technology, and in particular relates to an intelligent concentration device for stem cell exosomes. Background Technology

[0002] Stem cell exosomes are natural nanoparticles with a variety of biological activities and are widely used in medicine, pharmaceuticals and other fields. The process of concentrating exosomes usually involves increasing the concentration of a large number of exosome media from a low level to the required level for subsequent research and application.

[0003] In existing technologies, membrane filtration is commonly used for filtration and concentration. Although it can effectively separate exosomes, the concentration efficiency is low due to the limitations of the pore size and filtration principle of the filter membrane. Furthermore, the exosome media are mostly stored in tanks and connected to the pump body through pipes. When multiple tanks are installed, the liquid supply pipes need to be moved and assembled horizontally on the top of multiple tanks. When the liquid supply pipes are connected to tanks located on both sides, the pipe extension is long, which leads to the process of media suction. In addition, the liquid supply pipes are prone to bends when they are led out. In the traditional field, peristaltic pumps are used to drive the liquid to flow through the pipes, but due to pipe bends or fluid dynamic limitations, the stability of the liquid supply is often affected, reducing the concentration efficiency of exosomes. There is room for improvement. Utility Model Content

[0004] The purpose of this invention is to solve the problems that when connecting to tanks located on both sides, the long extension of the pipeline leads to the process of media suction, which easily affects the pumping efficiency of the medium, and the bending of the liquid supply pipeline at the time of exiting the pipeline affects the fluid throughput. Therefore, an intelligent concentration device for stem cell exosomes is proposed.

[0005] To achieve the above objectives, this utility model adopts the following technical solution: an intelligent concentration device for stem cell exosomes, comprising a frame, an equipment box installed inside the frame, a linear module provided on the front side of the equipment box, and a pumping device installed on one side of the linear module's movable seat, and further comprising:

[0006] Multiple tanks, with storage tanks installed on the outside of the tanks, and the storage tanks connected to the frame;

[0007] A filter module assembly is connected to the front side of the equipment box, and the filter module assembly is used to perform tangential flow filtration and concentration of the exosome media inside and outside the tank;

[0008] A liquid supply pipe is coiled around the outside of the pumping equipment drive component. One end of the liquid supply pipe extends to the top of one side of the tank, and the other end of the liquid supply pipe extends to the liquid inlet of the filter module assembly.

[0009] A sterile liquid storage assembly is connected to the front side of the equipment box. The sterile liquid storage assembly is connected to the filtrate outlet of the filtration module assembly through a filtrate pipeline.

[0010] An extension base is connected to one side of the pumping equipment. The extension base is equipped with a buffer component to buffer the liquid supply pipe led out by the pumping equipment. The extension base has through grooves at the positions corresponding to the inlet and outlet of the liquid supply pipe to accommodate the liquid supply pipe, so as to prevent the liquid supply pipe from extending and bending and affecting the sample throughput.

[0011] As a further description of the above technical solution:

[0012] The buffer component includes:

[0013] The first buffer roller is slidably connected to the inner cavity of the expansion seat on the side near the pumping equipment outlet;

[0014] The second buffer roller is located on the side of the first buffer roller. It contacts the upward-extending liquid supply pipe through the second buffer roller. The compression and folding of the medium flow channel are reduced by the support of the first and second buffer rollers and the liquid supply pipe.

[0015] As a further description of the above technical solution:

[0016] Also includes:

[0017] Two sliding rods are connected opposite each other to the top of the first buffer roller. The sliding rods are slidably connected to the sliding holes opened on the top side of the expansion seat. A plate is connected between the two sliding rods at their ends outside the expansion seat.

[0018] The second spring is sleeved on the outside of the slide rod, and its two ends are respectively connected to the corresponding positions on one side of the plate and the extension seat.

[0019] As a further description of the above technical solution:

[0020] It also includes: a collar, which is connected to one side of the second buffer roller via a rod. Two collars are respectively fitted onto the slide rod at corresponding positions. A first spring is connected to the bottom of the collar, and the other end of the first spring is connected to the top of the first buffer roller. The first spring is fitted onto the slide rod. The relative height of the second buffer roller is controlled by the sliding of the collar outside the slide rod to adapt to the contact strength of the liquid supply pipeline.

[0021] As a further description of the above technical solution:

[0022] It also includes: a bearing seat sleeved outside the second buffer roller, the bearing seat being connected to one side of the collar, and bearing seats being connected to both ends of the second buffer roller, the bearing seats being slidably connected to the stroke grooves opened on both sides of the bearing seat.

[0023] As a further description of the above technical solution:

[0024] Also includes:

[0025] A movable block is slidably connected to the travel groove and in contact with the bearing seat. A telescopic rod is connected to one side of the movable block, and the other end of the telescopic rod is connected to one side of the inner cavity of the travel groove. A fourth spring is sleeved on the telescopic rod. The two ends of the fourth spring are respectively connected to the outside of the movable block and the telescopic rod. A flexible limiting liquid supply pipe is slidably limited in the travel groove through the second buffer roller.

[0026] As a further description of the above technical solution:

[0027] It also includes: a sleeve, which is slidably connected to the liquid supply pipe outlet groove at the top of the expansion base. The liquid supply pipe passes through the sleeve. A third spring is connected to both sides of the sleeve. The other end of the third spring is connected to one side of the inner cavity of the outlet groove of the expansion base.

[0028] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0029] 1. In this utility model, the pumping equipment can be laterally displaced through the rear linear module, which is beneficial for adapting to the arrangement position of multiple tanks on the front side through the movable pumping equipment. Furthermore, the peristaltic pump can meet the peristaltic pumping needs in different lateral positions, making it convenient to process samples of different volumes and concentrations, improving the efficiency of media transportation and replacement. The buffer component can ensure that the liquid supply pipeline always maintains a partial bend when the tanks are connected in multiple lateral positions, maintaining the stability of media transportation and avoiding the impact of the coiled and bent liquid supply pipeline on the concentration and filtration efficiency in traditional technology.

[0030] 2. In this utility model, by designing a first buffer roller and a second buffer roller, when the liquid supply pipe extends from the pipe cavity of the peristaltic pump of the pumping equipment to the extension seat, it can extend upward along the inner cavity of the extension seat into the filter module assembly. The first buffer roller can fully limit the liquid supply pipe by utilizing the elastic force of the second spring and absorb the bending stress of the liquid supply pipe. The first buffer roller can cooperate with the lateral second buffer roller to increase the bending radius of the liquid supply pipe, which helps to avoid excessive bending of the liquid supply pipe from affecting the medium transportation, ensuring the smooth flow of liquid in the pipe, reducing disturbance to the sample, and improving the stability of the medium transportation in the tank. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the overall structure of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0032] Figure 2 This is a schematic diagram of the transverse structure of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0033] Figure 3 The present utility model proposes Figure 2 Enlarged structural diagram of part A in the middle;

[0034] Figure 4 This is a schematic diagram of the buffer component structure of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0035] Figure 5 This is a schematic diagram of the second buffer roller structure of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0036] Figure 6 This is a schematic diagram of the side assembly structure of the equipment box of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0037] Figure 7 This is a schematic diagram of the expansion base assembly structure of an intelligent concentration device for stem cell exosomes proposed in this utility model;

[0038] Figure 8 This is a schematic diagram of the disassembled structure of the expansion seat of an intelligent concentration device for stem cell exosomes proposed in this utility model.

[0039] Legend: 1. Frame; 2. Storage tank; 3. Tank body; 4. Pumping equipment; 5. Extension seat; 6. Buffer assembly; 601. First buffer roller; 602. Slide rod; 603. First spring; 604. Collar; 605. Second buffer roller; 606. Second spring; 607. Tube sleeve; 608. Third spring; 609. Moving block; 610. Telescopic rod; 611. Fourth spring; 612. Shaft seat; 7. Linear module; 8. Filter module assembly; 9. Aseptic liquid storage assembly; 10. Drive motor; 11. Equipment box; 12. Liquid supply pipeline; 13. Return pipe; 14. Filtrate pipeline. Detailed Implementation

[0040] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0041] Please see Figures 1-8 This utility model provides a technical solution: an intelligent concentration device for stem cell exosomes, including a frame 1, an equipment box 11 installed inside the frame 1, a linear module 7 on the front side of the equipment box 11, and a pumping device 4 installed on one side of the movable seat of the linear module 7. A drive motor 10 is installed on one side of the linear module 7, and the drive motor 10 drives the transmission screw to move the movable seat to drive the pumping device 4. This part is a mature technology in related fields and will not be described further.

[0042] It also includes: multiple tanks 3, with storage tanks 2 installed on the outside of the tanks 3, and the storage tanks 2 connected to the frame 1;

[0043] The filter module assembly 8 is connected to the front side of the equipment box 11. The filter module assembly 8 performs tangential flow filtration and concentration of the secretory media inside and outside the tank 3.

[0044] The liquid supply pipe 12 is coiled around the outside of the pumping device 4 drive component. One end of the liquid supply pipe 12 extends to the top of one side of the tank 3, and the other end of the liquid supply pipe 12 extends to the liquid inlet of the filter module assembly 8.

[0045] The sterile liquid storage component 9 is connected to the front side of the equipment box 11. The sterile liquid storage component 9 is connected to the filtrate outlet of the filter module component 8 through the filtrate pipe 14.

[0046] The expansion seat 5 is connected to one side of the pumping device 4. The expansion seat 5 is equipped with a buffer component 6, which buffers the liquid supply pipe 12 led out from the pumping device 4. The expansion seat 5 is provided with through grooves to accommodate the liquid supply pipe 12 at the positions corresponding to the inlet and outlet of the liquid supply pipe 12, so as to avoid the liquid supply pipe 12 from extending and bending and affecting the sample throughput.

[0047] In this embodiment, the pumping device 4 is a peristaltic pump, which rotates to make the medium in the pipeline flow to the filter module assembly 8.

[0048] The filter module component 8 extends to the top reflux port of the tank 3 via the reflux pipe 13, which is used to return unfiltered exosome media to the tank 3. The filter module component 8 improves the separation and concentration ratio of exosomes without losing the sample through the tangential flow filtration mechanism. The unfiltered sample is returned to the tank 3 in a timely manner through the reflux pipe 13, reducing sample loss.

[0049] The filter module component 8 has a membrane pack. After the sample in the tank 3 is pumped to the membrane pack by the pumping device 4, the membrane pack filters the tangential filtrate from the outlet into the storage container of the sterile storage component 9. The unfiltered sample is returned to the tank 3 through the return pipe 13. The membrane pack can be quickly replaced by the insertion port reserved in the filter module component. Furthermore, the membrane pack in the filter component is a well-known technology in the field. It can be tubular or modular, and can be replaced with microfiltration or ultrafiltration membrane packs according to process requirements.

[0050] Furthermore, the liquid storage container in the sterile liquid storage assembly 9 is connected to the end of the pipeline via a quick-connect interface, which facilitates the retrieval and placement of the exosomes after concentration. The quick-connect interface can be a corresponding bayonet or threaded part, which is not limited here. This part is a well-known technology in the field and will not be described further.

[0051] The buffer component 6 includes:

[0052] The first buffer roller 601 is slidably connected to the inner cavity of the expansion seat 5 on the side near the outlet of the pumping device 4;

[0053] The second buffer roller 605 is disposed on the side of the first buffer roller 601. The second buffer roller 605 contacts the upwardly extending liquid supply pipe 12, and the compression of the medium flow channel is reduced by the support of the first buffer roller 601 and the second buffer roller 605 and the liquid supply pipe 12.

[0054] Also includes:

[0055] Two slide rods 602 are connected to the top of the first buffer roller 601, and the slide rods 602 are slidably connected in the sliding hole opened on the top side of the extension base 5. A plate is connected between the two slide rods 602 at one end outside the extension base 5.

[0056] The second spring 606 is sleeved on the outside of the slide rod 602, and the two ends of the second spring 606 are respectively connected to the corresponding positions on one side of the plate and the extension seat 5;

[0057] A collar 604 is connected to one side of the second buffer roller 605 via a rod. Two collars 604 are respectively sleeved on the outside of the corresponding slide rod 602. A first spring 603 is connected to the bottom of the collar 604. The other end of the first spring 603 is connected to the top of the first buffer roller 601. The first spring 603 is sleeved on the outside of the slide rod 602. The relative height of the second buffer roller 605 is controlled by the sliding of the collar 604 on the outside of the slide rod 602 to adapt to the contact strength of the liquid supply pipe 12.

[0058] Specifically: By designing the first buffer roller 601 and the second buffer roller 605, when the liquid supply pipe 12 extends to the extension seat 5 through the pipe cavity of the peristaltic pump of the pumping device 4, it can extend upward along the inner cavity of the extension seat 5 into the filter module assembly 8. At this time, the first buffer roller 601 can fully limit the liquid supply pipe 12 by utilizing the elastic force of the second spring 606 and absorb the bending stress of the liquid supply pipe 12. Furthermore, the first buffer roller 601 can cooperate with the lateral second buffer roller 605 to increase the bending radius of the liquid supply pipe 12, which helps to avoid excessive bending of the liquid supply pipe 12 from affecting the medium transportation and improves the stability of the medium transportation in the tank 3.

[0059] Furthermore, the pumping device 4 can be laterally displaced through the rear linear module 7, which is beneficial for adapting to the separate arrangement of multiple tanks 3 on the front side through the movable pumping device 4, improving the efficiency of medium transportation and replacement. In addition, the buffer component 6 can keep the liquid supply pipe 12 partially bent when connected to multiple lateral tanks 3, maintain the stability of medium transportation, and avoid the impact of the coiled and bent liquid supply pipe 12 on the concentration and filtration efficiency in the traditional technology.

[0060] Furthermore, through the design of the second buffer roller 605, the second buffer roller 605 can slide outside the slide rod 602 via the rear collar 604. The sliding of the second buffer roller 605 can adapt to the flexible energy absorption of the liquid supply pipe 12 with different hardness, and avoid excessive compression that would cause the liquid supply pipe 12 to be compressed and affect the internal medium flow.

[0061] It also includes: a bearing seat 612 sleeved on the outside of the second buffer roller 605, the bearing seat 612 being connected to one side of the collar 604, and bearing seats being connected to both ends of the second buffer roller 605, the bearing seats being slidably connected to the stroke grooves opened on both sides of the bearing seat 612;

[0062] The movable block 609 is slidably connected to the travel groove and in contact with the bearing seat. A telescopic rod 610 is connected to one side of the movable block 609, and the other end of the telescopic rod 610 is connected to one side of the inner cavity of the travel groove. A fourth spring 611 is sleeved on the telescopic rod 610. The two ends of the fourth spring 611 are respectively connected to the outer side of the movable block 609 and the telescopic rod 610. The sliding flexible limiting liquid supply pipe 12 is located in the travel groove through the second buffer roller 605.

[0063] Specifically: By sliding the second buffer roller 605 within the bearing seat 612, the second buffer roller 605 can collapse and avoid excessive compression by sliding the bearing seats on both sides within the bearing seat 612. This helps to further reduce the flexible compression on the liquid supply pipe 12, improve the limit adaptability, and the collar 604 that moves outside the slide rod 602 can pull the first spring 603. The first spring 603 can absorb the swaying and offset of the second buffer roller 605 with its own elasticity, reduce the impact of the medium flowing in the liquid supply pipe 12 on the placement position of the liquid supply pipe 12, and improve the placement stability.

[0064] In another embodiment, it also includes: a sleeve 607, which is slidably connected to the outlet groove of the liquid supply pipe 12 at the top of the expansion base 5. The liquid supply pipe 12 passes through the sleeve 607. A third spring 608 is connected to both sides of the sleeve 607. The other end of the third spring 608 is connected to one side of the inner cavity of the outlet groove of the expansion base 5.

[0065] The third spring 608 and the sleeve 607 are designed to maintain the stability of the liquid supply pipe 12 as it moves through the sleeve 607 in the outlet groove.

[0066] In this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance; the term "multiple" refers to two or more unless otherwise explicitly defined. The terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; "linking" can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0067] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A smart concentration device for stem cell exosomes, comprising a frame (1), an equipment box (11) installed inside the frame (1), a linear module (7) provided on the front side of the equipment box (11), and a pumping device (4) installed on one side of the movable seat of the linear module (7), characterized in that, Also includes: Multiple tanks (3), with storage tanks (2) installed on the outside of the tanks (3), and the storage tanks (2) are connected to the frame (1); The filter module assembly (8) is connected to the front side of the equipment box (11) and the filter module assembly (8) performs tangential flow filtration and concentration of the secretory media inside and outside the tank (3); The liquid supply pipe (12) is coiled around the outside of the pumping device (4) drive component. One end of the liquid supply pipe (12) extends to the top of one side of the tank (3), and the other end of the liquid supply pipe (12) extends to the liquid inlet of the filter module assembly (8). The sterile liquid storage assembly (9) is connected to the front side of the equipment box (11). The sterile liquid storage assembly (9) is connected to the filtrate outlet of the filter module assembly (8) through the filtrate pipe (14). An extension base (5) is connected to one side of the pumping device (4). A buffer assembly (6) is provided inside the extension base (5) to buffer the liquid supply pipe (12) led out from the pumping device (4). The extension base (5) is provided with a through groove to accommodate the liquid supply pipe (12) at the position corresponding to the entry and exit of the liquid supply pipe (12), so as to avoid the liquid supply pipe (12) from extending and bending and affecting the sample throughput.

2. The intelligent concentration device for stem cell exosomes according to claim 1, characterized in that, The buffer component (6) includes: The first buffer roller (601) is slidably connected to the side of the inner cavity of the expansion seat (5) near the outlet of the pumping device (4); The second buffer roller (605) is located on the side of the first buffer roller (601). The second buffer roller (605) contacts the upwardly extending liquid supply pipe (12). The compression of the medium flow channel is reduced by the support of the first buffer roller (601) and the second buffer roller (605) and the liquid supply pipe (12).

3. The intelligent concentration device for stem cell exosomes according to claim 2, characterized in that, Also includes: Slide rod (602), two slide rods (602) are connected to the top of the first buffer roller (601) opposite each other. The slide rods (602) are slidably connected to the sliding hole opened on the top side of the extension seat (5). A plate is connected between the two slide rods (602) at one end outside the extension seat (5). The second spring (606) is sleeved on the outside of the slide rod (602), and the two ends of the second spring (606) are respectively connected to the corresponding positions on one side of the plate and the extension seat (5).

4. The intelligent concentration device for stem cell exosomes according to claim 3, characterized in that, Also includes: A collar (604) is connected to one side of the second buffer roller (605) via a rod. Two collars (604) are respectively sleeved on the outside of the corresponding slide rod (602). A first spring (603) is connected to the bottom of the collar (604). The other end of the first spring (603) is connected to the top of the first buffer roller (601). The first spring (603) is sleeved on the outside of the slide rod (602). The relative height of the second buffer roller (605) is controlled by the sliding of the collar (604) outside the slide rod (602) to adapt to the contact strength of the liquid supply pipe (12).

5. The intelligent concentration device for stem cell exosomes according to claim 3, characterized in that, Also includes: A bearing seat (612) is sleeved on the outside of the second buffer roller (605). The bearing seat (612) is connected to one side of the collar (604). Both ends of the second buffer roller (605) are connected to bearing seats, which are slidably connected to the stroke grooves opened on both sides of the bearing seat (612).

6. The intelligent concentration device for stem cell exosomes according to claim 5, characterized in that, Also includes: A movable block (609) is slidably connected in the stroke groove and in contact with the bearing seat. A telescopic rod (610) is connected to one side of the movable block (609), and the other end of the telescopic rod (610) is connected to one side of the inner cavity of the stroke groove. A fourth spring (611) is sleeved on the telescopic rod (610). The two ends of the fourth spring (611) are respectively connected to the outer side of the movable block (609) and the telescopic rod (610). A sliding flexible limiting liquid supply pipe (12) is connected in the stroke groove through the second buffer roller (605).

7. The intelligent concentration device for stem cell exosomes according to claim 1, characterized in that, Also includes: The sleeve (607) is slidably connected to the outlet groove of the liquid supply pipe (12) at the top of the expansion base (5). The liquid supply pipe (12) passes through the sleeve (607). A third spring (608) is connected to both sides of the sleeve (607). The other end of the third spring (608) is connected to one side of the outlet groove of the expansion base (5).

8. The intelligent concentration device for stem cell exosomes according to claim 1, characterized in that, Also includes: The return port of the filter module assembly (8) extends through the return pipe (13) to the return port at the top of the tank (3) for liquid discharge and return.

Images