A multi-directional cradle

Abstract

The utility model discloses a kind of multidirectional shaking table, it is related to cell ball preparation equipment technical field, for preparing mesenchymal stem cell ball, including base, motion platform and tray, motion platform is installed on base and can reciprocating swing movement;Tray is installed on the motion platform for carrying the container of culture cell ball, it can be translated relative to the upper surface of the motion platform;When preparing mesenchymal stem cell ball, the swing of the motion platform and the plane motion of tray relative to the upper surface of motion platform are coupled, and the container for culture cell ball generates compound motion track.The utility model generates complex compound motion track by the coupling of two kinds of motion, so as to effectively promote cell aggregation, improve the forming quality and uniformity of cell ball.

Description

Technical Field

[0001] This utility model relates to the field of cell sphere preparation equipment technology, specifically a multi-directional shaker. Background Technology

[0002] Three-dimensional culture technology for mesenchymal stem cell spheres has shown great potential in drug toxicology research, disease model construction, and regenerative medicine. Compared with two-dimensional culture, three-dimensional cell spheres can better simulate the real state of cells in vivo in terms of tissue structure, intercellular communication, and differentiation function.

[0003] Among numerous methods for preparing cell spheres, the dynamic suspension culture method based on a shaker has become the mainstream due to its ability to mass-produce cells and its good sphere uniformity. The core of this method lies in using the physical motion generated by the shaker to keep the cells suspended and aggregate in the culture medium.

[0004] In the existing technology, ordinary horizontal shakers or circular shakers (which provide single-dimensional motion) have been widely used, but they have obvious drawbacks: their single motion trajectory is difficult to effectively prevent cells from adhering to the wall in the early stage of culture, and the resulting cell spheres are of uneven size. Utility Model Content

[0005] The purpose of this invention is to provide a multi-directional shaking bed to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A multidirectional shaker for preparing mesenchymal stem cell spheres includes a base, a motion platform, and a tray. The motion platform is mounted on the base and can perform reciprocating oscillating motion. The tray is mounted on the motion platform to hold the cultured cell spheres and can translate relative to the upper surface of the motion platform. During the preparation of mesenchymal stem cell spheres, the oscillation of the motion platform and the planar motion of the tray relative to the upper surface of the motion platform are coupled, generating a composite motion trajectory for the cultured cell spheres.

[0008] Preferably, the middle part of the motion platform is rotatably connected to the base via a rotating shaft, and when the motion platform swings, it reciprocates around the rotating shaft.

[0009] Preferably, the trajectory of the pallet's translational motion relative to the upper surface of the motion platform is circular.

[0010] Compared with the prior art, the beneficial effects of this utility model are:

[0011] This invention couples two motions to create a complex composite motion trajectory in the cell culture container, thereby effectively promoting cell aggregation and improving the forming quality and uniformity of cell spheres.

[0012] This invention uses a combination of circular motion and crank oscillation to achieve a gentler shaking and reversing motion, which reduces the damage to cell spheres caused by the shear force generated by sudden reversals in traditional shakers. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of a multi-directional shaking table.

[0014] Figure 2 This is a schematic diagram of the structure at the bottom of the motion platform in a multi-directional shaker.

[0015] Figure 3 This is a schematic diagram of the base structure in a multi-directional shaking bed.

[0016] Figure 4 This is a schematic diagram of the structure of the top of the motion platform in a multi-directional shaking bed.

[0017] Figure 5 This is a schematic diagram of the crankshaft structure in a multi-directional shaking machine. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1-5 In this embodiment of the present invention, a multi-directional shaker for preparing mesenchymal stem cell spheres includes: a base 1; a motion platform 2, mounted on the base 1 and capable of reciprocating oscillation; and a tray 3, mounted on the motion platform 2 and used to hold the cultured cell spheres, capable of translational motion relative to the upper surface of the motion platform 2. During the preparation of mesenchymal stem cell spheres, the oscillation of the motion platform 2 and the planar motion of the tray 3 relative to the upper surface of the motion platform 2 are coupled, resulting in a composite motion trajectory for the container used to culture the cell spheres.

[0020] This invention couples two motions to create a complex composite motion trajectory in the cell culture container, thereby effectively promoting cell aggregation and improving the forming quality and uniformity of cell spheres.

[0021] Specifically, the middle part of the motion platform 2 is rotatably connected to the base 1 via a rotating shaft 4. When the motion platform 2 swings, it reciprocates around the rotating shaft 4.

[0022] In order to drive the swing of the motion platform 2, a first drive mechanism is installed on the base 1 to drive the motion platform 2 to reciprocate around the rotating shaft 4.

[0023] Specifically, the first drive mechanism includes a drive disk 5 that is mounted on the base 1 and can rotate around its own axis, and a connecting rod 6 that connects the drive disk 5 and one end of the motion platform 2. One end of the connecting rod 6 is hinged to one end of the motion platform 2, and the other end is hinged to the drive disk 5 at a position off-center. When the drive disk 5 rotates, it drives the motion platform 2 to reciprocate around the rotating shaft 4 through the connecting rod 6.

[0024] Furthermore, the drive disk 5 is rotatably mounted on the base 1 via a bracket and is driven to rotate by a first drive motor fixed on the base 1.

[0025] The trajectory of the pallet 3 in its translational motion relative to the upper surface of the motion platform 2 is circular.

[0026] In order to drive the translational movement of the tray 3, a second drive mechanism is installed on the motion platform 2 for driving the tray 3 to translate relative to the upper surface of the motion platform 2.

[0027] Specifically, the second drive mechanism includes a double crank structure mounted on the motion platform 2 and a second drive motor (not shown in the figure) for driving the double crank structure to rotate. The power output end of the double crank structure is connected to the tray 3, thereby driving the tray 3 to perform translational motion on the upper surface of the motion platform 2.

[0028] The double crank structure includes two crank shafts 7 of the same shape. The crank shafts 7 are right-angled Z-shaped crank shafts. One end of the crank shaft 7 is rotatably connected to the motion platform 2, and the other end is rotatably connected to the tray 3.

[0029] The right-angle Z-shaped crankshaft 7 includes two connecting sections that connect the motion platform 2 and the tray 3 respectively, as well as a transition section connecting the two connecting sections. The transition sections of the two crankshafts 7 are arranged parallel to each other.

[0030] It should be noted that the overall dimensions of the shaker in this application are approximately 300*200*170. When selecting the first drive motor and the second drive motor, a DC geared motor can be used. Attention should also be paid to the size of the motor. In this application, both the first drive motor and the second drive motor can be selected from the YS-37GB520 geared motor of Dongguan Yongsheng Motor Co., Ltd. The electrical components in this shaker are powered by the municipal power supply system.

[0031] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention. Furthermore, the contents not described in detail in this specification are all prior art known to those skilled in the art.

Claims

1. A multi-directional shaker for preparing mesenchymal stem cell spheroids, characterized by, include: Base (1); The motion platform (2) is mounted on the base (1) and can perform reciprocating swing motion; The tray (3), a container mounted on the motion platform (2) for carrying cultured cell spheres, can translate relative to the upper surface of the motion platform (2); In the preparation of mesenchymal stem cell spheres, the swing of the motion platform (2) and the planar motion of the tray (3) relative to the upper surface of the motion platform (2) are coupled to generate a compound motion trajectory for the container used to culture the cell spheres.

2. A multi-directional cradle as claimed in claim 1, wherein, The middle part of the motion platform (2) is rotatably connected to the base (1) through a rotating shaft (4). When the motion platform (2) swings, it swings back and forth around the rotating shaft (4).

3. A multi-directional cradle as claimed in claim 2, wherein, The base (1) is equipped with a first drive mechanism for driving the motion platform (2) to reciprocate around the rotating shaft (4).

4. A multi-directional cradle as claimed in claim 3, wherein, The first drive mechanism includes a drive disk (5) that is mounted on the base (1) and can rotate around its own axis, and a connecting rod (6) for connecting the drive disk (5) and one end of the motion platform (2). One end of the connecting rod (6) is hinged to one end of the motion platform (2), and the other end is hinged to a position of the drive disk (5) that is off-center. When the drive disk (5) rotates, the connecting rod (6) drives the motion platform (2) to reciprocate around the rotating shaft (4).

5. A multi-directional cradle as claimed in claim 4, wherein, The drive disk (5) is rotatably mounted on the base (1) via a bracket and is driven to rotate by a first drive motor fixed on the base (1).

6. A multi-directional cradle as defined in claim 1, wherein, The trajectory of the tray (3) in translational motion relative to the upper surface of the motion platform (2) is circular.

7. A multi-directional cradle as claimed in claim 1 or 6, wherein, The motion platform (2) is equipped with a second drive mechanism for driving the tray (3) to translate relative to the upper surface of the motion platform (2).

8. A multi-directional cradle as claimed in claim 7, wherein, The second drive mechanism includes a double crank structure mounted on the motion platform (2) and a second drive motor for driving the double crank structure to rotate. The power output end of the double crank structure is connected to the tray (3), thereby driving the tray (3) to perform translational motion on the upper surface of the motion platform (2).

9. A multi-directional cradle as claimed in claim 8, wherein, The double crank structure includes two crank shafts (7) of the same shape. The crank shafts (7) are right-angled Z-shaped crank shafts. One end of the crank shaft (7) is rotatably connected to the motion platform (2), and the other end is rotatably connected to the tray (3).

10. A multi-directional cradle as claimed in claim 9, wherein, The right-angle Z-shaped crankshaft (7) includes two connecting sections that connect the motion platform (2) and the tray (3) respectively, and a transition section connecting the two connecting sections. The transition sections of the two crankshafts (7) are arranged parallel to each other.

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