Portable cell culture bag

Abstract

The utility model discloses a portable cell culture bag, solve the problem that traditional passive culture bag liquid supplementing and exhaust share single channel leak easily, it is difficult to operate, exhaust is slow, and the pollution risk is high. It includes the bag body that breathable water -tight and edge is equipped with heat seal sealing part, bag body opening, export; inlet wear sets up composite pipeline, and the flexible separation membrane of waterproof and breathable in the pipe separates independent liquid inlet cavity way and exhaust cavity way, and liquid inlet cavity way supplies liquid supplementing, and exhaust cavity way prevents back flow, and separation membrane bends and seals liquid inlet when meeting liquid, and prevents liquid leakage and reduces the operation threshold. The inner end of composite pipeline bag is connected with the flow guide box, and the flat liquid guide port on the box faces the bag wall and reduces liquid impact on cells, and the bottom exhaust port borrows the gravity and improves the exhaust efficiency, and shortens the time -consuming.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, and in particular to a convenient cell culture bag. Background Technology

[0002] Cell culture bags are the core devices for in vitro cell culture, and can be divided into two categories according to the gas exchange method: active and passive. Active cell culture bags require specialized equipment for gas regulation, which is complex to operate and limited in application scenarios. Passive cell culture bags rely on their own breathable and water-resistant membrane material to achieve bidirectional exchange of oxygen and carbon dioxide, without the need for additional equipment. They have the advantages of convenient operation and strong adaptability to various scenarios, and are widely used in routine cell culture in laboratories, small-scale biopharmaceutical trials, and field research sample processing.

[0003] The structure of a conventional passive cell culture bag mainly includes: a bag body made of breathable but waterproof membrane material, a heat-sealed part at the edge of the body, an inlet for replenishing fluid and venting air, and an outlet for sampling and draining fluid. The inlet and outlet are usually connected to a single-channel pipe.

[0004] However, in use, existing cell culture bags share the same single-channel interface for replenishment and venting. When manually squeezing the bag to vent, the force needs to be precisely controlled to avoid the culture medium overflowing with the gas. This makes the operation difficult, and novices are prone to leakage. Moreover, each venting operation is time-consuming and inefficient. Furthermore, the interface needs to be exposed multiple times during the repeated adjustment of the squeezing force, which increases the risk of microbial contamination and affects the stability and sterility of cell culture. Utility Model Content

[0005] The main purpose of this invention is to provide a convenient cell culture bag, which aims to solve the problems of traditional passive cell culture bags, such as easy leakage due to the single channel shared by replenishment and venting, high operation threshold, long venting time, and high risk of microbial contamination.

[0006] To achieve the above objectives, this utility model proposes a convenient cell culture bag, comprising a bag body made of an air-permeable but water-impermeable membrane material, with a heat-sealing part at its edge, and an inlet and an outlet on the bag body; further comprising:

[0007] A composite pipe, one end of which is fixed to the inlet, is used to allow liquid to enter the bag and simultaneously allow air inside the bag to be discharged to the outside.

[0008] A flexible separator membrane, which is waterproof and breathable, extends along the length of the composite pipe and divides the internal space of the composite pipe into independent liquid inlet and exhaust channels.

[0009] The liquid inlet channel is a through structure with open ends. One end is located on the outside of the bag body to form a liquid replenishment port, and the other end extends into the bag body to form a first connecting port. The exhaust channel is a non-through structure with one end closed and the other end open. The closed end is located on the outside of the bag body, and the open end is located in the bag body to form a second connecting port.

[0010] A flow guide box is fixedly connected to one end of the composite pipe located inside the bag. The flow guide box is provided with a liquid guide port and an exhaust port. The liquid guide port is connected to a first connecting port, and the exhaust port is connected to a second connecting port.

[0011] In one possible implementation, the two ends of the flexible separator are respectively connected to the two ends of the composite pipe; when liquid enters the exhaust cavity, the flexible separator can bend towards the liquid inlet cavity and fit against the inner wall of the liquid inlet cavity to seal the first connection port.

[0012] In one possible implementation, the liquid guide port is flat and is located on the side of the guide box facing the inner wall of the bag body. The first connecting port of the liquid inlet channel is connected to the liquid guide port through the cavity of the guide box.

[0013] In one possible implementation, a plurality of vents are provided at the bottom of the flow guide box, and the vents are located below the liquid guide port.

[0014] In one possible implementation, the bottom surface of the flow guide box is provided with a plurality of evenly distributed conical protrusions, the plurality of conical protrusions are arranged on the same side as the exhaust port, and the plurality of conical protrusions and the plurality of exhaust ports are staggered.

[0015] In one possible implementation, a return pipe is provided between the composite pipe and the bag body, the return pipe connecting the exhaust chamber and the bottom area inside the bag body.

[0016] In one possible implementation, the flow guide box is provided with a support plate located at the liquid guide port. The support plate is inclined and is used to support a section of flexible separator membrane located at the liquid guide port.

[0017] In one possible implementation, the support plate is provided with a plurality of through holes.

[0018] In one possible implementation, a drain pipe is fixedly installed at the outlet of the bag, with a Luer sealing connector at one of its outer ends.

[0019] Compared with the prior art, the beneficial effects of this application are as follows:

[0020] This portable cell culture bag, through its integrated design of composite tubing, a flexible separator membrane, and a flow guide box, effectively solves the core problems of operational safety and efficiency inherent in traditional passive cell culture bags. The flexible separator membrane within the composite tubing extends along its length to form independent inlet and outlet channels. This physical isolation structure fundamentally changes the drawback of traditional single-channel designs where inlet and outlet channels share a common interface, ensuring that inlet and outlet processes do not interfere with each other and completely avoiding culture medium overflow caused by improper force control during manual outlet expulsion. The flexible separator membrane is made of waterproof and breathable material. When liquid accidentally enters the outlet channel, the membrane can bend towards the inlet channel and conform to the inner wall, automatically sealing the first connection point of the inlet channel. This self-adaptive sealing mechanism, requiring no additional mechanical parts, lowers the technical threshold for novice operators and enhances the safety of the culture process.

[0021] The flow guide box and composite pipeline are fixedly connected at their ends within the bag body, and the differentiated layout of their liquid inlet and vent further optimizes the liquid-gas separation efficiency. The liquid inlet faces the inner wall of the bag and is flat, reducing the impact of liquid on the bag body during replenishment; the vent is located at the bottom of the flow guide box and below the liquid inlet, allowing gas to be preferentially discharged by gravity, significantly shortening the single venting time. Simultaneously, the integrated design of the flow guide box and composite pipeline reduces the number of interfaces, and combined with the dynamic sealing function of the flexible separator membrane, reduces the risk of microbial contamination caused by repeated exposure of interfaces during operation, better meeting the stringent requirements of aseptic culture. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0023] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0024] Figure 2 This is a cross-sectional view of the present invention;

[0025] Figure 3 This is a structural diagram of the composite pipeline of this utility model;

[0026] Figure 4 for Figure 3 Enlarged view at point A;

[0027] Figure 5 This is a cross-sectional view of the composite pipe of this utility model;

[0028] Figure 6 This is a structural diagram of the support plate of this utility model;

[0029] Explanation of icon numbers:

[0030] 1. Bag body; 2. Heat-sealed part; 3. Inlet; 4. Outlet; 5. Composite pipe; 6. Flexible separator membrane; 7. Liquid inlet channel; 8. Exhaust channel; 9. First connecting port; 10. Second connecting port; 11. Flow guide box; 12. Liquid guide port; 13. Exhaust port; 14. Conical protrusion; 15. Return pipe; 16. Support plate; 17. Through hole; 18. Luer sealing joint; 19. Drainage conduit.

[0031] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0033] Reference Figures 1-6 This utility model proposes a portable cell culture bag with the bag body 1 as the core load-bearing structure. The bag body 1 is made of a breathable but waterproof membrane material. The principle is that the membrane material has a special microporous structure, which allows only the bidirectional permeation of oxygen and carbon dioxide required for cell metabolism, while completely blocking the leakage of culture medium. This satisfies the cell's respiration needs and avoids liquid leakage. The heat-sealing part 2 at the edge of the bag body 1 is formed by melting the edge of the membrane material at high temperature and cooling it to solidify. The thermoplasticity of the membrane material forms an integrated seal, which effectively prevents leakage or invasion of external microorganisms during the culture process and ensures a sterile environment inside the bag.

[0034] like Figure 1-2 As shown, the inlet 3 and outlet 4 on the bag body 1 serve the functions of replenishing liquid and venting gas, and draining liquid and sampling, respectively, providing a basic channel for fluid flow. A fixed composite pipe 5 is installed at the inlet 3 of the bag body 1, which is used to realize liquid transportation and gas discharge.

[0035] like Figure 5 As shown, a flexible separator 6 extends along the length of the pipe. The separator is made of waterproof and breathable material. It physically separates the inside of the pipe into an independent liquid inlet channel 7 and an exhaust channel 8, preventing liquid from flowing into the exhaust channel 8 and preventing gas from entering the liquid inlet channel 7.

[0036] The liquid inlet channel 7 is a through structure with open ends. The outer end forms a liquid replenishment port for connecting to an external liquid replenishment device, and the inner end forms a first connecting port 9. The through structure controls the unidirectional transport of liquid from the outside to the inside. The exhaust channel 8 is a non-through structure with one end closed and the other end open. The outer end is closed to prevent backflow of outside air, and the inner end forms a second connecting port 10 to ensure that the air inside the bag can only be discharged in one direction, further ensuring the stability of the environment inside the bag.

[0037] like Figure 3-4 As shown, at one end of the composite pipe 5 located inside the bag body 1, a flow guide box 11 is fixedly connected. Its core function is to optimize the efficiency of liquid guidance and gas discharge, and to avoid the fluid directly impacting the cells or blocking the channels.

[0038] The liquid guide port 12 on the flow guide box 11 is connected to the first connecting port 9 of the liquid inlet channel 7, and the vent port 13 is connected to the second connecting port 10 of the vent channel 8, forming a complete "liquid replenishment-flow guidance-venting" path. Among them, the liquid guide port 12 is designed to be flat and facing the inner wall of the bag body 1. Through the flat liquid guide port 12, the flow rate is slowed down by increasing the cross-sectional area of ​​the liquid outflow, and the liquid is guided to flow towards the wall surface, so that the culture medium flows smoothly into the bottom of the bag along the wall surface, avoiding direct impact on suspended or adherent cells and reducing mechanical damage to cells.

[0039] Multiple exhaust ports 13 are provided and located at the bottom of the flow guide box 11 and below the liquid guide port 12. By utilizing the physical property that the density of gas is less than that of liquid, the compressed air inside the bag naturally gathers in the flow guide box 11 and enters the exhaust channel 8 from the bottom exhaust port 13. Multiple exhaust ports 13 can also increase the exhaust area, significantly shorten the exhaust time, and solve the problem of long exhaust time in traditional culture bags.

[0040] Meanwhile, several conical protrusions 14 on the bottom surface of the flow guide box 11 are on the same side as the exhaust port 13 and are staggered. The principle is that the protrusions support the space between the flow guide box 11 and the bag body 1, preventing the bag body 1 from sticking to the bottom surface and blocking the exhaust port 13. At the same time, they disperse the liquid to prevent it from accumulating and covering the exhaust port 13, ensuring that the exhaust is continuous and smooth.

[0041] In addition, the flexible separator membrane 6 is fixedly connected to both ends of the composite pipe 5. When liquid enters the exhaust channel 8, the liquid pressure will cause the membrane to elastically deform towards the liquid inlet channel 7 until it adheres to the inner wall of the liquid inlet channel 7. By physically blocking and sealing the first connecting port 9, the effect is to automatically prevent the liquid from continuing to flow out, avoiding leakage caused by improper force control in traditional operation. Even novices can reduce the error rate. After the pressure is restored, the membrane can be elastically reset without affecting subsequent operations. At the same time, it also allows users to squeeze and discharge the gas in the bag 1 more quickly.

[0042] Furthermore, the return pipe 15 between the composite pipe 5 and the bag body 1 is located at the upper end of the exhaust chamber 8. When the user squeezes the bag body 1 to expel air, there is no need to precisely control the force as with traditional culture bags. Even if the force is slightly too great and some liquid enters the exhaust chamber 8, the return pipe 15 can quickly guide this liquid back to the bottom of the bag body 1, allowing the user to squeeze with confidence to quickly expel the air. At the same time, the liquid forms a circulation flow between the exhaust chamber 8 and the bottom of the bag body 1 through the return pipe 15. During the flow, the tiny bubbles in the liquid will gather with the circulation and rise to the exhaust chamber 8, and finally be discharged with the exhaust, effectively reducing the bubble content in the culture medium and preventing bubbles from affecting cell attachment or metabolism. In addition, if pressure is generated in the exhaust chamber 8 due to temporary accumulation of liquid, the return pipe 15 can serve as a pressure release channel, allowing the liquid to flow back in time, preventing the pressure in the chamber from being too high and squeezing the flexible separator 6, preventing the separator from being damaged or deformed due to excessive force, and ensuring the long-term stability of the liquid-gas isolation function.

[0043] In addition, such as Figure 6 As shown, the inclined support plate 16 at the liquid inlet 12 inside the flow box 11 supports the flexible separator membrane 6 by tilting, which allows the liquid in the liquid inlet 7 to flow smoothly out of the liquid inlet 12, avoiding residue and preventing excessive deformation of the membrane material caused by liquid impact during replenishment, thus preventing the liquid from flowing out smoothly.

[0044] The through-hole 17 on the support plate 16 ensures that during venting, the liquid inside the bag 1 will first pass through the vent hole and through-hole 17 to squeeze the separator membrane, thereby deforming the separator membrane to seal the liquid guide port 12.

[0045] The drain tube 19 at outlet 4 of bag body 1 has a Luer sealing connector 18 at the outer end with a tapered interface and threaded locking structure. The principle is to achieve a sealed connection by tightly fitting the interface and fixing it with threads. This can prevent leakage and microbial invasion during drainage or sampling, and meets the requirements of aseptic operation.

[0046] When using the device, the user first performs the fluid replenishment operation: connect the external sterile fluid replenishment device to the fluid replenishment port of the composite pipeline 5. After starting the fluid replenishment, the culture medium flows along the inlet channel 7, enters the guide box 11 through the first connecting port 9, flows from the flat guide port 12 to the inner wall of the bag body 1, and then flows smoothly into the bottom of the bag body 1 along the wall. During this process, the exhaust channel 8 is not involved in the gas flow because the outer end is closed and the second connecting port 10 on the inner end does not participate in the gas flow. It is only used as a backup channel. Moreover, the orientation and shape design of the guide port 12 can prevent the culture medium from directly impacting the pre-cultured cells in the bag and reduce mechanical damage to the cells.

[0047] After the fluid replenishment is complete, the user performs the venting operation: gently squeeze the bag 1 by hand. The reduced space inside the bag creates pressure, causing the air inside the bag to gather in the lower pressure area of ​​the guide box 11. The air then enters the second connecting port 10 of the venting channel 8 through multiple vent ports 13 at the bottom of the guide box 11, and then exits from the outside of the bag 1 along the venting channel 8. Since the return pipe 15 connects the venting channel 8 to the bottom of the bag 1, the user does not need to precisely control the squeezing force. Even if the squeezing force is slightly too strong and some culture medium enters the venting channel 8, this liquid will be returned through the return pipe. The return flow from pipe 15 to the bottom of bag 1 avoids liquid waste and allows users to safely squeeze the bag to quickly expel air. At the same time, the circulation of liquid between the exhaust channel 8 and the bottom of bag 1 carries away tiny air bubbles dissolved in the culture medium. These air bubbles are discharged from the exhaust channel 8 along with the air, reducing the impact of air bubbles inside the bag on cell adhesion and nutrient absorption. If liquid temporarily accumulates in the exhaust channel 8, causing the pressure to rise, the return pipe 15 can also release the pressure in time, preventing excessive pressure from damaging the flexible separator membrane 6 and ensuring the stability of the liquid-gas isolation function.

[0048] In the accompanying drawings of this embodiment, the same or similar reference numerals correspond to the same or similar components. In the description of this application, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the accompanying drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0049] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A portable cell culture bag, comprising a bag body (1), the bag body (1) being made of an air-permeable but water-impermeable membrane material, having a heat-sealing part (2) at its edge, and having an inlet (3) and an outlet (4) on the bag body (1); characterized in that, Also includes: A composite pipe (5) is provided at one end and fixed to the inlet (3). The composite pipe (5) is used to allow liquid to enter the bag body (1) and at the same time to allow air in the bag body (1) to be discharged to the outside. A flexible separator (6) is made of waterproof and breathable membrane. The flexible separator (6) extends along the length of the composite pipe (5) and divides the internal space of the composite pipe (5) into an independent liquid inlet channel (7) and an exhaust channel (8). Among them, the liquid inlet channel (7) is a through structure with open ends. One end is located outside the body of the bag (1) to form a liquid replenishment port, and the other end extends into the body of the bag (1) to form a first connecting port (9); the exhaust channel (8) is a non-through structure with one end closed and the other end open. The closed end is located outside the body of the bag (1), and the open end is located inside the body of the bag (1) to form a second connecting port (10); A flow guide box (11) is fixedly connected to one end of the composite pipe located inside the bag body (1). The flow guide box (11) is provided with a liquid guide port (12) and an exhaust port (13). The liquid guide port (12) is connected to the first connecting port (9), and the exhaust port (13) is connected to the second connecting port (10).

2. The portable cell culture bag according to claim 1, characterized in that, The two ends of the flexible separator (6) are respectively connected to the two ends of the composite pipe (5); when liquid enters the exhaust cavity (8), the flexible separator (6) can bend towards the liquid inlet cavity (7) and fit against the inner wall of the liquid inlet cavity (7) to seal the first communication port (9).

3. The portable cell culture bag according to claim 1, characterized in that, The liquid guide port (12) is flat and is located on the side of the guide box (11) facing the inner wall of the bag body (1). The first connecting port (9) of the liquid inlet channel (7) is connected to the liquid guide port (12) through the cavity of the guide box (11).

4. A convenient cell culture bag according to claim 2, characterized in that, Several exhaust ports (13) are provided, which are located at the bottom of the flow guide box (11) and the exhaust ports (13) are located below the liquid guide port (12).

5. A convenient cell culture bag according to claim 4, characterized in that, The bottom surface of the flow guide box (11) is provided with a number of evenly distributed conical protrusions (14), and the number of conical protrusions (14) are arranged on the same side as the exhaust port (13), and the number of conical protrusions (14) and the number of exhaust ports (13) are staggered.

6. A convenient cell culture bag according to claim 1, characterized in that, A return pipe (15) is provided between the composite pipe (5) and the bag body (1), and the return pipe (15) connects the exhaust chamber (8) and the bottom area inside the bag body (1).

7. A convenient cell culture bag according to claim 4, characterized in that, The flow guide box (11) is provided with a support plate (16) located at the liquid guide port (12). The support plate (16) is inclined and is used to support a section of flexible separator membrane (6) located at the liquid guide port (12).

8. A convenient cell culture bag according to claim 7, characterized in that, The support plate (16) is provided with several through holes (17).

9. A convenient cell culture bag according to claim 1, characterized in that, A drain pipe (19) is fixedly installed at the outlet (4) of the bag (1), and a Luer sealing connector (18) is provided at the outer end of the pipe.

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