A novel suspension cell culture flask with internal regulation culture system

Abstract

The utility model provides a novel can interiorly adjust the culture system's suspension cell culture bottle, including culture bottle, the inside left side of culture bottle is installed with first baffle, the middle part of first baffle vertical end left side is installed with second baffle, and first baffle and second baffle are fixedly connected with the end of two support columns respectively, and the first area is formed between the left side of first baffle vertical end and the right side of second baffle vertical end, and the second area is formed between the lower side of first baffle horizontal end and culture bottle, and the third area is formed between the lower side of second baffle horizontal end, the upper side of first baffle horizontal end and the left inner wall of culture bottle, and the design solves the problem of needing to replace the larger culture medium bottle to culture in the later period. However, in the process of culture, too many operations can increase the risk of cell contamination, the utility model structure is reasonable, under the premise of not changing experimental operation steps, can simplify the operation steps of cell, improve operation efficiency, can also reduce the risk of cell contamination, make the culture process more smoothly.

Description

Technical Field

[0001] This invention relates to a novel suspension cell culture flask with an internally adjustable culture system, belonging to the field of cell culture flask technology. Background Technology

[0002] When culturing suspension cells such as NK or DC-CIK, PBMCs need to be extracted from blood to activate and expand the culture to reach the desired quantity. During the culture process, the culture medium needs to be continuously replenished to meet the nutritional requirements for cell growth, depending on the cell proliferation. Initially, due to the small amount of culture medium, only T25 or T75 culture flasks can be used for inoculation. Later, as the amount of culture medium increases, T25 or T75 culture flasks are no longer sufficient for the later culture conditions, and the culture medium needs to be transferred to T175 culture flasks for further culture.

[0003] This type of operation has the following drawbacks: 1. At the beginning of inoculation, the number of PBMCs extracted from blood is limited. Inoculating at the recommended cell density results in a limited amount of culture medium. If an excessively large culture flask is used, the medium may not cover the entire bottom, hindering cell growth. 2. Suspension cells are density-dependent, thriving at high densities and deteriorating at low densities. Excessive inoculation medium can also impede cell growth. During culture, as cells proliferate, their numbers increase, requiring continuous replenishment of culture medium to meet their nutritional needs. Therefore, larger culture flasks are needed later in the culture process. However, excessive manipulation during culture increases the risk of cell contamination. Therefore, a novel suspension cell culture flask with an internally adjustable culture system is urgently needed to address these problems. Utility Model Content

[0004] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a novel suspension cell culture flask with an internally adjustable culture system to solve the problems mentioned in the background technology. This utility model has a reasonable structure and good practicality. Without changing the experimental operation steps, it can simplify the cell operation steps, improve the operation efficiency, reduce the risk of cell contamination, and make the culture process smoother.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a novel suspension cell culture flask with an internally adjustable culture system, comprising a culture flask, wherein support columns are respectively installed on the left and right sides inside the culture flask, a first baffle is installed on the left side inside the culture flask, a second baffle is installed on the middle of the left side of the vertical end of the first baffle, the first baffle and the second baffle are respectively fixed to the ends of the two support columns, a first region is formed between the left side of the vertical end of the first baffle and the right side of the vertical end of the second baffle, a second region is formed between the lower part of the horizontal end of the first baffle and the culture flask, a third region is formed between the lower part of the horizontal end of the second baffle, the upper part of the horizontal end of the first baffle and the left inner wall of the culture flask, the width of the first baffle is greater than the width of the second baffle, the upper surface of the first baffle and the upper surface of the second baffle are coplanar, and an inclined surface is provided at the upper rear side of the culture flask.

[0006] Furthermore, a culture medium exchange port is formed between the left side of the vertical end of the second baffle and the left side of the upper end and the left inner wall of the culture bottle.

[0007] Furthermore, a liquid outlet is formed between the right side of the vertical end of the first baffle, the right side of the upper end, and the right inner wall of the culture bottle.

[0008] Furthermore, an external threaded cylinder is installed at the upper center of the culture flask, and the external threaded cylinder is in communication with the inside of the culture flask.

[0009] Furthermore, the outer thread of the external threaded cylinder is connected to a rotating cap.

[0010] Furthermore, the culture flask is made of cyclic olefin copolymer material.

[0011] The beneficial effects of this utility model: This utility model provides a novel suspension cell culture flask with an internally adjustable culture system. Because this utility model adds a culture flask, a rotating cap, an external threaded cylinder, a support column, a first baffle, and a second baffle, our design improvements and actual use have shown that this device can simplify the cell operation steps and improve the operation efficiency without changing the experimental operation steps, while also reducing the risk of cell contamination and making the culture process smoother. Attached Figure Description

[0012] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0013] Figure 1 This is a three-dimensional schematic diagram of the overall structure of a suspension cell culture flask with a novel internally adjustable culture system according to the present invention.

[0014] Figure 2 This is a three-dimensional schematic diagram of the culture flask structure of a novel internally adjustable culture system for suspended cell culture flasks according to the present invention.

[0015] Figure 3 This is a three-dimensional cross-sectional view of a novel internally adjustable culture system for a suspended cell culture flask according to the present invention.

[0016] Figure 4 This is a three-dimensional cross-sectional view of a suspension cell culture flask with a novel internally adjustable culture system according to the present invention.

[0017] Figure 5 This is a three-dimensional structural diagram of a suspension cell culture flask with a novel internally adjustable culture system according to this utility model, when placed for culture work.

[0018] In the diagram: 1-Cultivation flask, 2-Cultivation medium exchange port, 3-Second region, 4-Outlet, 5-Third region, 6-Rotating cap, 7-External threaded cylinder, 8-Support column, 9-First baffle, 10-Second baffle, 11-First region. Detailed Implementation

[0019] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0020] Please see Figures 1-5 This utility model provides a technical solution: a novel suspension cell culture flask with an internally adjustable culture system, comprising a culture flask 1, with support columns 8 installed on the left and right sides inside the culture flask 1, a first baffle 9 installed on the left side inside the culture flask 1, and a second baffle 10 installed at the middle of the left side of the vertical end of the first baffle 9. The first baffle 9 and the second baffle 10 are respectively fixed to the ends of the two support columns 8. A first region 11 is formed between the left side of the vertical end of the first baffle 9 and the right side of the vertical end of the second baffle 10. A second region 3 is formed between the lower horizontal end of the first baffle 9 and the culture flask 1. A third region 5 is formed between the lower horizontal end of the second baffle 10, the upper horizontal end of the first baffle 9, and the left inner wall of the culture flask 1. The width of the first baffle 9 is greater than the width of the second baffle 10. The upper surface of the first baffle 9 and the upper surface of the second baffle 1 are coplanar. The upper rear side of the culture flask 1 has a slope. This design solves the problem of needing to replace the culture flask with a larger culture medium flask for later culture. However, excessive operation during the culture process increases the risk of cell contamination.

[0021] In the first embodiment of this utility model: a culture medium exchange port 2 is formed between the left side of the vertical end of the second baffle 10, the left side of the upper end, and the inner left wall of the culture flask 1, facilitating the exchange of culture medium. An outlet port 4 is formed between the right side of the vertical end of the first baffle 9, the right side of the upper end, and the inner right wall of the culture flask 1, facilitating the discharge of culture medium. An externally threaded cylinder 7 is installed in the middle of the upper part of the culture flask 1, communicating with the interior of the culture flask 1, facilitating the addition of culture medium to the culture flask 1. A rotating cap 6 is threaded onto the outer side of the externally threaded cylinder 7, facilitating the sealing of the externally threaded cylinder 7. The culture flask 1 is made of cyclic olefin copolymer material. The use of cyclic olefin copolymer material for the culture flask 1 is due to its good light transmittance, which is beneficial for observation under a microscope.

[0022] As a second embodiment of this invention: During inoculation, PBMCs are extracted from the blood and counted. The required amount of culture medium is calculated based on the recommended cell density. The cells are resuspended in culture medium, and other relevant factors or reagents are added. The cap 6 is then unscrewed, and the culture medium is added to the first region 11 using an external pipette. The culture flask 1 must always be kept horizontal to prevent the culture medium from flowing into the third region 5. The cap 6 is then reset and the flask is placed in a CO2 incubator for culture. When it is necessary to change regions for culture, as the number of cells proliferates, more culture medium is needed to provide the nutrients required for cell growth. When the first region 11 can no longer meet the required amount of culture medium, the culture medium in the first region 11 is transferred to the third region 5 through the culture medium exchange port 2 using a pipette. When the capacity of the third region 5 is insufficient for the culture medium system, cells can be cultured simultaneously in the third region 5 and the first region 11. When neither the third region 5 nor the first region 11 can meet the requirements of the culture system, the culture flask is slightly upright to allow the culture medium to enter the second region 3. At this time, cell culture of a larger system is carried out simultaneously in the three regions. When collecting cells, the culture flask is flipped to the right and the culture medium is poured out from the outlet for the next step.

[0023] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It will be apparent to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0024] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A novel suspension cell culture flask with internal regulation of culture system, comprising a culture flask (1), characterized in that: The culture bottle (1) is equipped with support columns (8) on the left and right sides respectively. The culture bottle (1) is equipped with a first baffle (9) on the left side. The first baffle (9) is equipped with a second baffle (10) on the middle left side of the vertical end of the first baffle (9). The first baffle (9) and the second baffle (10) are respectively fixed to the ends of the two support columns (8). A first region (11) is formed between the left side of the vertical end of the first baffle (9) and the right side of the vertical end of the second baffle (10). A second region (3) is formed between the lower part of the horizontal end of the first baffle (9) and the culture bottle (1). A third region (5) is formed between the lower part of the horizontal end of the second baffle (10), the upper part of the horizontal end of the first baffle (9), and the left inner wall of the culture bottle (1). The width of the first baffle (9) is greater than the width of the second baffle (10). The upper surface of the first baffle (9) and the upper surface of the second baffle (10) are coplanar. The upper rear side of the culture bottle (1) is provided with an inclined surface.

2. The novel suspension cell culture flask with internal regulation culture system according to claim 1, characterized in that: The second baffle (10) forms a culture medium exchange port (2) between the left side of the vertical end and the left side of the upper end and the left inner wall of the culture bottle (1).

3. The novel suspension cell culture flask with internal regulation culture system according to claim 1, characterized in that: The first baffle (9) forms an outlet (4) between the right side of the vertical end and the right side of the upper end and the right inner wall of the culture bottle (1).

4. The novel suspension cell culture flask with internal regulation culture system according to claim 1, characterized in that: An external threaded cylinder (7) is installed in the middle of the upper part of the culture bottle (1), and the external threaded cylinder (7) is connected to the inside of the culture bottle (1).

5. The novel suspension cell culture flask with internal regulation culture system according to claim 4, characterized in that: The external threaded cylinder (7) is threaded with a rotating cap (6) on its outer side.

6. The novel suspension cell culture flask with internal regulation culture system according to claim 1, characterized in that: The culture flask (1) is made of cyclic olefin copolymer material.

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