An apparatus for cell vibration frequency detection

Abstract

The application discloses a device for cell vibration frequency detection and belongs to the technical field of biological medicine. The device for cell vibration frequency detection comprises a detection box body, a clamp is arranged in the detection box body, a containing container is fixed on the clamp, the containing container comprises a loading plate and a cover plate made of quartz, a cavity is arranged between the loading plate and the cover plate, an ITO conductive film is arranged at the bottom of the loading plate, a copper foil is arranged on the side of the loading plate deviating from the cavity, the copper foil is electrically connected with the ITO conductive film, the detection box body is provided with a spring needle, the spring needle is in abutment with the copper foil and is connected with a grounding wire, a microscope is arranged above the containing container, and an ultrasonic wave transmitter is arranged below the containing container. When the loading plate and the cover plate are rubbed and electrostatic, the electrostatic can be discharged, the stacking of cells with points is avoided, and the cell vibration detection precision is affected.

Description

Technical Field

[0001] This application relates to the field of biomedical technology, and more specifically, to a device for detecting cell vibrational frequencies. Background Technology

[0002] Cells are the basic structural and functional units of living organisms, and their physiological state directly reflects the health of the body. Recent studies have discovered that cells generate extremely weak mechanical vibrations during their life activities. These vibrations are closely related to factors such as cellular metabolic activity, membrane potential changes, and cytoskeleton dynamics. Normal cells and diseased cells differ significantly in their physiological structure and function, resulting in different vibrational frequency characteristics.

[0003] A search revealed that Chinese patent CN117554346A discloses a method for detecting the resonance frequency of cell nuclei. This method involves obtaining tumor cells from tumor tissue obtained during surgery, transfecting the tumor cells with lentivirus to label the cell nuclei, enabling them to emit bright fluorescence, and then exciting the labeled cells with ultrasound of different frequencies to find the intrinsic frequency of the tumor cell nuclei. This method can more accurately identify the resonance frequency of tumor cells, providing a guarantee for subsequent killing of tumor cells.

[0004] Cells are typically placed on glass plates and coverslips. During ultrasonic stimulation, friction occurs between the glass plates and coverslips, causing the cells to become charged and stack, affecting detection accuracy. Therefore, we propose a device for detecting cell vibrational frequencies. Utility Model Content

[0005] 1. The technical problems to be solved.

[0006] The purpose of this application is to provide a device for detecting cell vibrational frequency, in order to solve the problems mentioned in the background art.

[0007] 2. Technical solution.

[0008] This application is achieved through the following technical solution.

[0009] A device for detecting cell vibrational frequencies includes a detection chamber with a clamp inside. A container is fixed on the clamp. The container includes a carrier plate and a cover plate made of quartz material, with a cavity between the carrier plate and the cover plate. An ITO conductive film is provided at the bottom of the carrier plate, and a copper foil is provided on the side of the carrier plate away from the cavity. The copper foil is electrically connected to the ITO conductive film. The detection chamber has a spring needle that abuts against the copper foil and is connected to a grounding wire. A microscope is provided above the container, and an ultrasonic transmitter is provided below it.

[0010] As an optional solution to the technical solution in this application, the inner surface of the detection box is covered with sound-absorbing cotton.

[0011] As an optional solution to the technical solution of this application, a sealing shaft is rotatably connected to one side of the detection box. One side of the sealing shaft is located inside the detection box, and the other side is located outside the detection box. A through groove is provided on the sealing shaft in the radial direction. A control rod is slidably inserted into the through groove, and the control rod is connected and fixed to the clamp.

[0012] As an optional solution to the technical solution of this application, the detection box has an opening at the top, the clamp includes two symmetrically arranged clamping arms, the clamping arms are connected and fixed to the control rod, there is a gap between the two clamping arms, and slots are opened on the opposite side of the two clamping arms.

[0013] As an optional embodiment of the technical solution in this application, the slot is located above the control lever.

[0014] As an optional solution to the technical solution in this application, the inner wall of the slot is provided with an insulating layer.

[0015] As an optional solution to the technical solution of this application, the detection box is provided with a limiting structure inside, which is used to fix the position of the clamp between the microscope and the ultrasonic transmitter.

[0016] As an optional solution to the technical solution in this application, the spring pin is fixed on the limiting structure.

[0017] 3. Beneficial effects.

[0018] Compared with the prior art, the beneficial effects of this application are as follows.

[0019] 1) By setting an ITO conductive film and a spring pin, this application can achieve grounding of the carrier plate. When static electricity is generated due to friction between the carrier plate and the cover plate, the static electricity can be discharged to avoid cell charge stacking, which would affect the accuracy of cell vibration detection.

[0020] 2) By setting a sealing shaft and a control rod, this application allows users to adjust the position of the clamp from the outside of the testing chamber, preventing staff from putting their hands into the testing chamber, which could lead to contamination inside the testing chamber and affect the normal use of the optical equipment inside the testing chamber. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of a device used for detecting cell vibrational frequencies.

[0022] Figure 2 This is a schematic diagram of a fixture structure for a device used to detect cell vibration frequencies.

[0023] Figure 3 This is a schematic diagram of a carrier plate structure for a device used to detect cell vibration frequencies.

[0024] In the diagram: 1. Detection chamber; 101. Opening; 2. Fixture; 201. Clamping arm; 202. Slot; 3. Container; 301. Carrier plate; 302. Cover plate; 3011. ITO conductive film; 3012. Copper foil; 4. Microscope; 5. Ultrasonic transmitter; 6. Sealing shaft; 7. Control rod; 8. Limiting structure; 9. Spring pin. Detailed Implementation

[0025] The technical solution of this application will now be clearly and completely described in conjunction with the accompanying drawings.

[0026] Please see Figures 1 to 3 This application provides a device for detecting cell vibration frequency, including a detection chamber 1, a clamp 2 inside the detection chamber 1, a container 3 fixed on the clamp 2, the container 3 including a carrier plate 301 and a cover plate 302 made of quartz material, a cavity between the carrier plate 301 and the cover plate 302, an ITO conductive film 3011 at the bottom of the carrier plate 301, a copper foil 3012 on the side of the carrier plate 301 away from the cavity, the copper foil 3012 being electrically connected to the ITO conductive film 3011, a spring needle 9 in the detection chamber 1 abutting against the copper foil 3012 and connected to a grounding wire, a microscope 4 above the container 3 and an ultrasonic transmitter 5 below it.

[0027] In this technical solution, the output frequency of the ultrasonic transmitter 5 is 1kHz-10MHz. Fluorescently labeled cells are placed in the cavity between the carrier plate 301 and the cover plate 302. The container 3 is then fixed to the clamp 2. By adjusting the position of the clamp 2, the container 3 is placed between the microscope 4 and the transmitter 5, with the spring needle 9 contacting the copper foil 3012. The ultrasonic transmitter 5 emits continuously adjustable ultrasonic waves to apply ultrasonic excitation to the cells, and the vibration response of the fluorescently labeled cells is observed through the microscope 4, thus detecting the cell vibration frequency. During the detection process, the static electricity generated by the friction between the carrier plate 301 and the cover plate 302 can be discharged through the spring needle 9, preventing cell stacking from causing a shift in the detected cell vibration frequency. Simultaneously, the ITO conductive film 3011 shields the electric field generated by the ultrasonic transmitter 5 in the cavity, further increasing the accuracy of cell vibration frequency detection. Preferably, the thickness of both the carrier plate 301 and the cover plate 302 is 0.15 mm, and the thickness of the ITO conductive film 3011 is 145 nm, so that the light transmittance of the container 3 can meet the usage requirements.

[0028] As a preferred embodiment of this application, the inner surface of the test chamber 1 is covered with sound-absorbing cotton to absorb the ultrasonic waves generated by the ultrasonic transmitter 5, reduce the reflection of ultrasonic waves in the chamber 1, and weaken the interference to the test accuracy.

[0029] like Figure 2 As shown, a sealing shaft 6 is rotatably connected to one side of the testing chamber 1. One side of the sealing shaft 6 is located inside the testing chamber 1, and the other side is located outside the testing chamber 1. A through groove is formed radially on the sealing shaft 6, and a control rod 7 is slidably inserted into the through groove. The control rod 7 is connected and fixed to the clamp 2. The user can adjust the position of the clamp 2 from outside the testing chamber 1 using the control rod 7, so that the clamp 2 can be placed in an accurate position.

[0030] In a preferred embodiment of this application, the upper part of the testing box 1 is provided with an opening 101, and the clamp 2 includes two symmetrically arranged clamping arms 201. The clamping arms 201 are connected and fixed to the control rod 7, and a gap is provided between the two clamping arms 201. A slot 202 is provided on the opposite side of each of the two clamping arms 201. Preferably, the inner wall of the slot 202 is provided with an insulating layer to achieve insulation between the container 3 and the clamp 2; preferably, the slot 202 is located above the control rod 7. In this embodiment, the user can adjust the horizontal and vertical positions of the clamp 2 by using the control rod 7. When the control rod 7 is rotated upward, the slot 202 part of the clamp 2 can extend out of the opening 101 to the outside of the opening 101, so that the user can install the container 3 into the slot 202.

[0031] like Figure 3 As shown, the dimensions of the carrier plate 301 and the cover plate 302 are 25.0 ±0.1 mm × 8.0 ±0.1 mm × 0.15 ±0.02 mm. A 2 mm bare area is left between the ITO conductive film 3011 and the short side of the carrier plate 301 for insertion into the slot 202.

[0032] As a preferred embodiment of this application, the detection chamber 1 is provided with a limiting structure 8, which is used to fix the position of the clamp 2 between the microscope 4 and the ultrasonic transmitter 5, and the spring pin 9 is fixed on the limiting structure 8.

Claims

1. An apparatus for cell vibration frequency detection, characterized by: The device includes a detection box (1), inside which is a clamp (2), on which a container (3) is fixed. The container (3) includes a carrier plate (301) and a cover plate (302) made of quartz material. A cavity is provided between the carrier plate (301) and the cover plate (302). An ITO conductive film (3011) is provided at the bottom of the carrier plate (301). A copper foil (3012) is provided on the side of the carrier plate (301) away from the cavity. The copper foil (3012) is electrically connected to the ITO conductive film (3011). The detection box (1) is provided with a spring pin (9). The spring pin (9) abuts against the copper foil (3012) and is connected to a grounding wire. A microscope (4) is provided above the container (3), and an ultrasonic transmitter (5) is provided below it.

2. The device for cell vibration frequency detection according to claim 1, characterized in that: The inner surface of the testing box (1) is covered with sound-absorbing cotton.

3. The device for cell vibration frequency detection according to claim 1, characterized in that: A sealing shaft (6) is rotatably connected to one side of the detection box (1). One side of the sealing shaft (6) is located inside the detection box (1), and the other side is located outside the detection box (1). A through groove is provided in the radial direction of the sealing shaft (6), and a control rod (7) is slidably inserted in the through groove. The control rod (7) is connected and fixed to the clamp (2).

4. The device for cell vibration frequency detection according to claim 3, characterized in that: The detection box (1) has an opening (101) on the upper part. The clamp (2) includes two clamping arms (201) arranged symmetrically. The clamping arms (201) are connected and fixed to the control rod (7). There is a gap between the two clamping arms (201). Slots (202) are opened on the opposite side of the two clamping arms (201).

5. A device for cell vibration frequency detection according to claim 4, characterized in that: The slot (202) is located above the control lever (7).

6. The device for cell vibration frequency detection according to claim 4, characterized in that: The inner wall of the slot (202) is provided with an insulating layer.

7. The device for cell vibration frequency detection according to claim 1, characterized in that: The detection box (1) is provided with a limiting structure (8) inside, which is used to fix the position of the clamp (2) between the microscope (4) and the ultrasonic transmitter (5).

8. A device for cell vibration frequency detection according to claim 7, characterized in that: The spring pin (9) is fixed on the limiting structure (8).

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