Magnetic stand with locking structure

Abstract

The application relates to the field of flow fluorescence detection technology and discloses a magnetic stand with a locking structure, which is used for accommodating a multi-well enzyme-coated plate and comprises a square frame, an adjusting sheet, a first locking strap and a second locking strap. A plurality of limiting grooves are arranged in the square frame, and a first opening, a second opening, a third opening and a fourth opening are sequentially arranged at the four corners of the top of the square frame. The adjusting sheet is arranged in the square frame, and two horizontal sliding grooves are arranged on the adjusting sheet. One end of the first locking strap penetrates through the first opening and is fixed in the interior of the square frame, and the other end penetrates through the second opening and is slidably connected with the horizontal sliding groove. One end of the second locking strap penetrates through the third opening and is fixed in the interior of the square frame, and the other end penetrates through the fourth opening and is slidably connected with the other horizontal sliding groove. When the first locking strap and the second locking strap slide to be close to the middle part of the square frame, the first locking strap and the second locking strap are used for binding the multi-well enzyme-coated plate.

Description

Technical Field

[0001] This application relates to the field of flow cytometry detection technology, and in particular to a magnetic rack with a locking structure. Background Technology

[0002] Flow cytometry is a high-throughput immunoassay method that combines flow cytometry detection technology with fluorescent labeling technology. It is widely used in biomedical research, clinical diagnosis, drug development and other fields.

[0003] Flow cytometry is commonly used for parallel detection of multiple indicators (such as proteins, nucleic acids, and small molecules). A single experiment may require processing dozens to hundreds of samples, with each sample needing to be tested for multiple indicators. Therefore, multi-well microplates are often used for simultaneous reactions. During the sample incubation phase, the mixture needs to be washed 1-2 times. During the washing process, the magnetic rack containing the multi-well microplate needs to be inverted to discard the supernatant. However, existing magnetic racks cannot stably lock the microplate strips on the multi-well microplate, which can easily cause the strips to protrude, making the magnetic attraction unstable or even causing the entire strip to detach and lose the sample.

[0004] Therefore, improving the stability of multi-well microplates on a magnetic rack has become an urgent technical problem to be solved. Utility Model Content

[0005] The technical problem to be solved by this application is: how to improve the stability of multi-well microplates on a magnetic rack.

[0006] To address the aforementioned technical problems, this application provides a magnetic frame with a locking structure for accommodating multi-well microplates. The magnetic frame includes: a square frame with several limiting grooves inside, each groove having a magnetic element at its bottom; a first opening, a second opening, a third opening, and a fourth opening at the four upper corners of the square frame, the first opening, the second opening, the third opening, and the fourth opening being sequentially adjacent and all being strip-shaped holes; an adjustment plate placed inside the square frame near one end of the first opening and the fourth opening, the adjustment plate having two horizontal grooves corresponding to the first opening and the fourth opening respectively; a first locking strap, one end of which passes through the first opening and is fixed inside the square frame, the other end of which passes through the second opening and is slidably connected to the horizontal groove; a second locking strap, one end of which passes through the third opening and is fixed inside the square frame, the other end of which passes through the fourth opening and is slidably connected to the other horizontal groove; when the first locking strap and the second locking strap slide to near the middle of the square frame, the first locking strap and the second locking strap are used to bind the multi-well microplate.

[0007] In one embodiment, the magnetic frame further includes a knob, a vertical groove is provided in the square frame, the adjustment plate is slidably connected to the square frame through the vertical groove, and a vertical slot is provided on the end face of the square frame near the first opening and the fourth opening, the knob passes through the vertical slot and is threadedly connected to the adjustment plate.

[0008] In one embodiment, the knob is a hand-tightening nut.

[0009] In one embodiment, the side of the adjustment piece near the vertical slot is provided with an anti-slip texture.

[0010] In one embodiment, the square frame is further provided with another adjustment piece at one end near the second and third openings, and the two adjustment pieces have the same structure.

[0011] In one embodiment, the surface of the magnetic element is coated with an anti-corrosion coating, which is an epoxy resin or pyrene coating, and the thickness of the anti-corrosion coating is 5 to 20 micrometers.

[0012] In one embodiment, the width of the first opening, the second opening, the third opening, and the fourth opening is 2-5 mm, and the length is 10-20 mm.

[0013] In one embodiment, the first locking strap or the second locking strap is detachably connected to the adjustment tab.

[0014] Compared with the prior art, the magnetic frame with a locking structure described in this application has the following advantages:

[0015] The magnetic frame of this application embodiment, through the locking structure formed by the first and second locking straps combined with the horizontal sliding groove on the adjusting plate, can bring many significant beneficial effects.

[0016] On the one hand, this locking structure can accurately and stably bind the multi-well microplate. When locking is required, the first and second locking straps can slide in the horizontal groove to near the center of the square frame, firmly fixing the multi-well microplate and preventing unstable magnetic attraction or detachment during operations such as cleaning and inverting, thus ensuring sample safety and experimental stability and solving the problem of insufficient locking of existing magnetic frames.

[0017] On the other hand, because the first and second locking straps can slide within their respective horizontal grooves, the locking structure can flexibly switch between restrained and unrestrained states. When placing or removing the microplate, the locking straps can be slid away from the center to release the restraint, facilitating quick and easy plate placement and removal. Conversely, when locking is required, they can be quickly slid back to the restrained position, making the operation simple and efficient. This switchable feature ensures stability during locking while enhancing ease of use, making experimental operations smoother, adapting to the high-throughput detection requirements of flow cytometry, and improving overall experimental efficiency. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of a magnetic rack with a locking structure, as exemplarily shown in an embodiment of this application.

[0019] Figure 2 This is a cross-sectional schematic diagram of a magnetic rack with a locking structure, as exemplarily shown in an embodiment of this application.

[0020] Figure 3 This is a top view schematic diagram of a magnetic rack with a locking structure, as exemplarily shown in an embodiment of this application.

[0021] Figure label:

[0022] 1. Magnetic frame; 2. Multi-well microplate; 11. Square frame; 111. First opening; 112. Second opening; 113. Third opening; 114. Fourth opening; 115. Limiting groove; 12. Adjusting piece; 121. Horizontal slide; 122. Threaded connection hole; 13. First locking strap; 14. Second locking strap; 15. Knob; 116. Vertical slide; 117. Vertical strip hole. Detailed Implementation

[0023] The specific embodiments of this application will be described in further detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this application, but are not intended to limit the scope of this application.

[0024] In the description of this application, it should be understood that the terms "first," "second," etc., in the specification, claims, and accompanying drawings are intended to distinguish similar objects and are not used to describe a specific structure. It should be understood that such terms are interchangeable where appropriate so that embodiments of this application can be implemented in structures other than those illustrated or described. Furthermore, "comprising," "having," and any variations thereof are intended to cover non-exclusive inclusion. For example, a product or device comprising a series of components or units is not necessarily limited to those explicitly listed, but may also include other components or units not explicitly listed but inherent to these products or devices.

[0025] Flow cytometry, a product of the deep integration of flow cytometry detection and fluorescence labeling technologies, is a highly advantageous high-throughput immunoassay method in the life sciences. With its ability to simultaneously and accurately detect multiple target substances, this technology occupies an irreplaceable position in many key areas such as basic biomedical research, clinical disease diagnosis, and innovative drug development, providing researchers and healthcare professionals with efficient and comprehensive analytical data.

[0026] In practical applications, flow cytometry often needs to handle scenarios requiring the parallel detection of multiple indicators. Whether it's proteins, nucleic acids, or various small molecules, this technology can enable simultaneous analysis. A single experiment often processes dozens or even hundreds of samples, and each sample may require the detection of multiple indicators, which places extremely high demands on the high-throughput processing capabilities of the experimental equipment.

[0027] To meet the demands of such large-scale simultaneous reactions, multi-well microplates have become the core carrier in experiments. Their standardized well design can simultaneously accommodate large amounts of sample reaction systems, ensuring efficient experimental progress. In the sample incubation stage of flow cytometry reactions, to remove unbound impurities and improve detection sensitivity, the mixture typically requires 1-2 washing operations. A key step in the washing process is placing the multi-well microplate containing the sample on a magnetic rack, then inverting the rack to discard the supernatant.

[0028] However, existing magnetic holders have significant design flaws, failing to provide a stable locking effect for the ELISA strips on multi-well ELISA plates. During the inverted dispensing of supernatant, the ELISA strips are prone to displacement and protrusion. This not only causes instability in the magnetic attraction of the beads, affecting the washing effect, but more seriously, the entire ELISA strip may detach directly from the plate, resulting in the loss of valuable samples and adversely impacting the accuracy and reliability of experimental results.

[0029] Therefore, how to improve the existing magnetic rack to enhance the stability of multi-well microplates on the magnetic rack has become a key technical problem that urgently needs to be solved in current flow cytometry experiments.

[0030] Based on this, such as Figure 1 3D image Figure 2 Cross-sectional view and Figure 3 As shown in the top view, a preferred embodiment of this application provides a magnetic rack 1 with a locking structure. The magnetic rack 1 is used to accommodate a multi-well microplate 2. The magnetic rack 1 may include: a square frame 11, an adjustment piece 12, a first locking strap 13, and a second locking strap 14.

[0031] The square frame 11 has several limiting grooves 115, each with a magnetic element at its bottom. At the four upper corners of the square frame 11, there are four openings: a first opening 111, a second opening 112, a third opening 113, and a fourth opening 114. These openings are sequentially adjacent and are all strip-shaped holes. An adjusting piece 12 is placed inside the square frame 11 near one end of the first opening 111 and the fourth opening 114. The adjusting piece 12 has two horizontal sliding grooves 121 corresponding to the first opening 111 and the fourth opening 114, respectively. One end of a first locking strap 13 passes through the first opening 111 and is fixed inside the square frame 11. The other end of the first locking strap 13 passes through the second opening 112 and is slidably connected to the horizontal sliding groove 121. One end of the second locking strap 14 passes through the third opening 113 and is fixed inside the square frame 11. The other end of the second locking strap 14 passes through the fourth opening 114 and is slidably connected to another horizontal groove 121.

[0032] When the first locking tether 13 and the second locking tether 14 slide to the middle of the square frame 11, the first locking tether 13 and the second locking tether 14 are used to bind the multi-well microplate 2.

[0033] In the structure of the magnetic frame 1 described above, the locking structure formed by the first and second locking straps 14 combined with the horizontal slide groove 121 on the adjusting piece 12 can bring about many significant beneficial effects.

[0034] On the one hand, this locking structure can accurately and stably bind the multi-well microplate 2. When locking is required, the first and second locking straps 14 can slide in the horizontal groove 121 to near the middle of the square frame 11, firmly fixing the multi-well microplate 2 and avoiding unstable magnetic attraction or detachment during cleaning and inverting operations, thus ensuring sample safety and experimental stability and solving the problem of insufficient locking of the existing magnetic frame 1.

[0035] On the other hand, since the first and second locking straps 14 can slide within their respective horizontal grooves 121, the locking structure can flexibly switch between binding and unbinding. When placing or removing the microplate, the locking straps can be slid away from the center to release the binding, facilitating quick and easy plate placement and removal. When locking is required, they can be quickly slid back to the binding position, making the operation simple and efficient. This switchable feature ensures stability during locking while improving ease of use, making experimental operations smoother, adapting to the high-throughput detection requirements of flow cytometry, and improving overall experimental efficiency.

[0036] In one embodiment, the magnetic frame 1 further includes a knob 15, a vertical groove 116 is provided in the square frame 11, the adjusting plate 12 is slidably connected to the square frame 11 through the vertical groove 116, a vertical slot 117 is provided on the end face of the square frame 11 near the first opening 111 and the fourth opening 114, and the knob 15 is threadedly connected to the adjusting plate 12 through the vertical slot 117.

[0037] By adding a knob 15, a vertical slide 116, and a vertical slot 117 to the magnetic frame 1, the practicality and stability of the overall structure are further improved, bringing about many beneficial effects.

[0038] First, the adjustment plate 12 is slidably connected to the square frame 11 via the vertical slide groove 116, which can drive the locking strap to achieve vertical position adjustment. Combined with the horizontal adjustment function of the horizontal slide groove 121, it can adapt to enzyme-labeled plates and enzyme-labeled strips of different thicknesses, ensuring that the locking strap and enzyme-labeled strip are tightly attached. Figure 2 As shown, the knob 15 passes through the vertical strip hole 117 and connects to the threaded connection hole 122 on the adjustment piece 12. After tightening, the adjustment piece 12 can be firmly fixed in a specific position of the vertical slide groove 116, preventing the adjustment piece 12 from shifting due to vibration or tilting during the locking process. This ensures that the binding force of the locking strap remains stable, further reinforcing the locking effect on the enzyme-labeled plate and enzyme-labeled strip from the vertical direction.

[0039] Secondly, the vertical slide groove 116 provides a stable sliding trajectory for the adjusting plate 12. Combined with the vertical slot 117 limiting the knob 15, the experimenter can easily control the lifting height of the adjusting plate 12 via the knob 15, achieving fine adjustment of the locking strap in the vertical direction. This design not only meets the personalized needs for locking height in different experimental scenarios but also reduces the difficulty of operation, allowing even beginners to quickly master the adjustment techniques and ensure optimal locking every time.

[0040] Meanwhile, the threaded connection between knob 15 and adjustment plate 12 has excellent self-locking performance, preventing loosening even after long-term use and extending the equipment's lifespan. The vertical slot 117 is positioned to avoid the core reaction area, ensuring it does not affect the placement of the ELISA plate or the magnetic adsorption effect, while also providing ample space for knob 15 operation. This achieves an optimized balance between functionality and spatial layout, better adapting to the high-frequency cleaning and locking requirements of flow cytometry reactions.

[0041] In one embodiment, knob 15 is a hand-tightening nut. The hand-tightening nut requires no tools; experimenters can tighten or loosen it directly by hand, making operation more convenient and faster. This meets the needs of high-frequency adjustment of the locking structure in flow cytometry reactions, improving experimental efficiency.

[0042] In one embodiment, the adjusting plate 12 has an anti-slip texture on the side near the vertical slot 117. The anti-slip texture increases the friction between the hand and the adjusting plate 12, preventing the hand from slipping when adjusting the position of the adjusting plate 12 or tightening the knob 15, making the operation more stable and precise, and avoiding problems such as incomplete adjustment or insecure locking caused by slipping.

[0043] In one embodiment, the square frame 11 is further provided with another adjusting piece 12 near the second opening 112 and the third opening 113. The two adjusting pieces 12 have the same structure. The two adjusting pieces 12 support and adjust the locking strap from both sides, which can further enhance the binding force of the locking strap on the ELISA plate and the ELISA strip, making the locking more uniform and stable. At the same time, it improves the symmetry and balance of the locking structure, and adapts to the locking requirements of ELISA plates of different specifications.

[0044] In one embodiment, the surface of the magnetic element is coated with an anti-corrosion coating, which is an epoxy resin or pyrene coating, and the thickness of the anti-corrosion coating is 5 to 20 micrometers.

[0045] The surface of the magnetic element is coated with an anti-corrosion coating, which is an epoxy resin or pyrene coating, with a thickness of 5 to 20 micrometers. The anti-corrosion coating can effectively isolate the magnetic element from corrosive substances such as acid and alkali solutions and reagents that may come into contact with it in the experiment, delay the corrosion and aging of the magnetic element, extend its service life, ensure the long-term stability of the magnetic attraction force, and ensure the normal progress of the cleaning step in the flow cytometry reaction.

[0046] In one embodiment, the width of the first opening 111, the second opening 112, the third opening 113, and the fourth opening 114 is 2-5 mm, and the length is 10-20 mm.

[0047] The opening size range provides ample space for the locking strap to slide while preventing it from wobbling due to excessive size. It also prevents too much debris from entering the frame, ensuring stable operation of the locking structure and adapting to the size and activity requirements of the locking strap.

[0048] In one embodiment, the first locking strap 13 or the second locking strap 14 is detachably connected to the adjusting plate 12. When the locking strap is worn or aged, it can be easily removed from the adjusting plate 12 for replacement without replacing the entire adjusting plate 12 or the magnetic frame 1, thus reducing maintenance costs and extending the overall service life of the magnetic frame 1.

[0049] The working process of this application is as follows: In use, first place the multi-well ELISA plate 2 in the limiting groove 115 of the square frame 11. According to the specifications of the ELISA plate and ELISA strip, adjust the height of the locking system by sliding the adjusting piece 12 in the vertical slide groove 116. Then, use the horizontal slide groove 121 to adjust the horizontal position of the first locking strap 13 and the second locking strap 14 to ensure they adhere to the ELISA strip. Next, tighten the knob 15 (or hand-tighten the nut if it is a hand-tightening nut) to fix the adjusting piece 12, thus locking the ELISA plate and ELISA strip. After cleaning, loosen the knob 15 and slide the locking straps away from the ELISA plate to remove it. When the locking straps wear out, they can be replaced using the detachable connection structure. During long-term use, the magnetic components maintain stable magnetic attraction due to the anti-corrosion coating.

[0050] In summary, the embodiments of this application provide a magnetic rack 1 with a locking structure, which achieves stable fixation of the multi-well microplate 2 and the microplate strip through a multi-dimensional adjustable locking system. The designs of each embodiment optimize the performance of the magnetic rack 1 in terms of ease of operation, locking stability, structural durability, and ease of maintenance, thereby ensuring the safety of samples during the flow cytometry reaction cleaning process, improving experimental efficiency and result accuracy, reducing experimental costs, and meeting the needs of high-throughput detection.

[0051] The above description is only a preferred embodiment of this application. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of this application, and these improvements and substitutions should also be considered within the scope of protection of this application.

Claims

1. A magnetic stand having a locking structure, characterized by, The magnetic rack (1) is used to accommodate the multi-well microplate (2), and the magnetic rack (1) includes: A square frame (11) is provided with a plurality of limiting grooves (115) inside the square frame (11). The bottom of each of the plurality of limiting grooves (115) is provided with a magnetic element. A first opening (111), a second opening (112), a third opening (113) and a fourth opening (114) are respectively opened at the four corners of the upper part of the square frame (11). The first opening (111), the second opening (112), the third opening (113) and the fourth opening (114) are adjacent to each other and are all strip holes. Adjustment piece (12), the adjustment piece (12) is placed inside the square frame (11) at one end near the first opening (111) and the fourth opening (114), the adjustment piece (12) is provided with two horizontal sliding grooves (121) corresponding to the first opening (111) and the fourth opening (114) respectively; A first locking strap (13) has one end passing through the first opening (111) and fixed inside the square frame (11), and the other end passing through the second opening (112) and slidably connected to the horizontal groove (121). The second locking strap (14) has one end passing through the third opening (113) and fixed inside the square frame (11), and the other end passing through the fourth opening (114) and slidably connected to another horizontal groove (121). When the first locking tether (13) and the second locking tether (14) slide to the middle of the square frame (11), the first locking tether (13) and the second locking tether (14) are used to bind the multi-well microplate (2).

2. The magnetic stand of claim 1, wherein, The magnetic frame (1) also includes a knob (15). The square frame (11) is provided with a vertical groove (116). The adjusting plate (12) is slidably connected to the square frame (11) through the vertical groove (116). The square frame (11) has a vertical slot (117) on its end face near the first opening (111) and the fourth opening (114). The knob (15) passes through the vertical slot (117) and is threadedly connected to the adjusting plate (12).

3. The magnetic stand of claim 2, wherein, The knob (15) is a hand-tightening nut.

4. The magnetic stand of claim 2, wherein, The adjusting piece (12) has an anti-slip texture on the side near the vertical strip hole (117).

5. The magnetic stand of claim 1, wherein, The square frame (11) is provided with another adjustment piece (12) at one end near the second opening (112) and the third opening (113), and the two adjustment pieces (12) have the same structure.

6. The magnetic stand of claim 1, wherein, The surface of the magnetic element is covered with an anti-corrosion coating, which is an epoxy resin or pyrene coating, and the thickness of the anti-corrosion coating is 5 to 20 micrometers.

7. The magnetic stand of claim 1, wherein, The width of the first opening (111), the second opening (112), the third opening (113), and the fourth opening (114) is 2-5 mm, and the length is 10-20 mm.

8. The magnetic stand of claim 1, wherein, The first locking strap (13) or the second locking strap (14) is detachably connected to the adjusting piece (12).

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