High-efficiency in-vitro hatching device for crayfish

CN224775818UActive Publication Date: 2026-09-22HUNAN NORMAL UNIVERSITY +1
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Patent Information

Application Number
CN202522346720.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-11-05
Publication Date
2026-09-22
Estimated Expiration
2035-11-05

AI Technical Summary

Technical Problem

[0004]然而,市面上常见的离体孵化装置效率较低,一个孵化器或孵化盒搭配一套除菌设备,导致孵化成本高

Benefits of technology

1、本实用新型的小龙虾高效离体孵化装置,采用水体杀菌组件,可以杀灭水体中的细菌等有害物质,有利于虾卵的孵化。

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Abstract

The utility model discloses a kind of crawfish efficient in-vitro incubator, including incubator, first water tank and second water tank, the incubator is connected first water tank by a overflow water pipe, the first water tank is connected by a nanometer bubble machine for increasing oxygen content in water body and the second water tank, the second water tank is connected by water inlet pipe and the incubator, water body sterilization assembly is equipped in the first water tank.The crawfish efficient in-vitro incubator of the utility model can solve the problems of high cost, low efficiency and bacterial contamination of conventional in-vitro incubator, resulting in low survival rate of egg grains.
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Description

Technical Field

[0001] This utility model belongs to the field of aquaculture technology, and in particular relates to a crayfish cultivation device. Background Technology

[0002] Crayfish, scientifically known as *Procambarus clarkii*, also called red swamp crayfish or swamp crayfish, is a widely farmed freshwater crustacean. Crayfish are oviparous; after mating, the female lays fertilized eggs on her abdominal appendages for incubation. During incubation, the female provides oxygen by moving her abdominal appendages.

[0003] Under natural conditions, the hatching rate of crayfish can be affected by various factors, such as ambient temperature, water quality, and maternal health. In vitro hatching can improve the hatching rate by optimizing hatching conditions, thereby increasing both the hatching rate and survival rate. In vitro hatching can be carried out in a sterile or low-bacterial environment, reducing the risk of pathogen infection to embryos and larvae, and helping to improve larval survival. During in vitro hatching, specific fertilized eggs can be selected for hatching to facilitate genetic improvement and the breeding of superior varieties, thus improving aquaculture efficiency.

[0004] However, commercially available in vitro incubation devices are inefficient, requiring a sterilization system for each incubator or hatching box, resulting in high incubation costs. Furthermore, many incubation devices use a "series" connection method for their incubation water source, leading to incomplete sterilization and rapid spread of pathogens among the eggs. In addition, most incubation devices simulate the incubation process of shrimp eggs in the mother's body through mechanical shaking, causing mechanical collisions and resulting in egg death. Utility Model Content

[0005] The technical problem to be solved by this utility model is to overcome the deficiencies and defects mentioned in the background art above, and to provide a high-efficiency in vitro hatching device for crayfish that is low in cost, thoroughly sterilized, and has a high hatching rate.

[0006] To solve the above-mentioned technical problems, the technical solution proposed by this utility model is as follows: A high-efficiency in vitro hatching device for crayfish includes an incubator, a first water tank, and a second water tank. The incubator is connected to the first water tank via an overflow pipe. The first water tank is connected to the second water tank via a nanobubble generator for increasing the oxygen content in the water. The second water tank is connected to the incubator via an inlet pipe. The first water tank is equipped with a water sterilization component.

[0007] In the aforementioned high-efficiency in vitro crayfish incubation device, preferably, multiple incubators are arranged side-by-side, and the water inlet pipe is correspondingly provided with multiple branch pipes, each branch pipe being equipped with a valve. By arranging multiple incubators side-by-side, the device efficiency can be improved, and one sterilization component can act on multiple incubators simultaneously, thus reducing device costs. Each branch pipe supplies water and oxygen to the crayfish eggs in the incubator, and each branch pipe has an inherent on / off valve to control the water inflow (the valve can adjust the water flow rate).

[0008] Preferably, the above-mentioned high-efficiency in vitro incubation device for crayfish also includes a water collection slide, and the overflow outlet pipes of multiple incubators are connected to the water collection slide. The water collection slide is connected to the first water tank through a water pipe.

[0009] In the aforementioned high-efficiency in vitro crayfish hatching device, preferably, the first water tank is equipped with a mesh screen, and the outlet of the drain pipe is located within the mesh screen. The mesh screen can be used to separate debris, dead crayfish eggs, etc., from the water.

[0010] In the aforementioned high-efficiency in vitro crayfish incubation device, preferably, the outlet of the branch pipe extends downwards to the lower part of the incubator. The location of the branch pipe's outlet in the lower part of the incubator facilitates the circulation of water within the incubator.

[0011] In the aforementioned high-efficiency in vitro incubation device for crayfish, preferably, the incubator includes a main body cylinder and an upper trough. The upper trough surrounds the top of the main body cylinder, the top of the main body cylinder and the top of the upper trough are flush, the bottom of the main body cylinder is arc-shaped, and the overflow pipe is located at the bottom of the upper trough. This structure facilitates the overflow of dead eggs from the top of the main body cylinder and their collection in the upper trough.

[0012] In the above-mentioned high-efficiency in vitro incubation device for crayfish, preferably, the water sterilization component includes an ultraviolet lamp installed in the first water tank.

[0013] In the aforementioned high-efficiency in vitro crayfish hatching device, preferably, the water sterilization component includes an ozone generator for timed ozone supply, the outlet of which is connected to the inlet of the nanobubble generator. The outlet of the nanobubble generator may include a diversion pipe, allowing water to be evenly distributed into the second water tank. The ozone generator primarily adds ozone continuously to the water, killing bacteria and diseases, ensuring a favorable hatching environment, and providing a good guarantee for a high hatching rate. The ozone generator can be equipped with a timer switch, allowing for continuous adjustment of the on / off time.

[0014] In this invention, the sterilization component includes an ultraviolet lamp and an ozone generator. Using the ultraviolet lamp for conventional disinfection, followed by combined sterilization with a nanobubble generator and ozone generator, can significantly improve the sterilization efficiency of the water and thus increase the hatching rate. The ultraviolet lamp, ozone generator, and nanobubble generator can all be existing conventional equipment.

[0015] In the aforementioned high-efficiency in vitro crayfish hatching device, preferably, a siphon connecting pipe is provided between the first and second water tanks, and a degassing device is connected to the siphon connecting pipe. The siphon connecting pipe ensures water level balance between the first and second water tanks, and the degassing device prevents siphon connecting pipe failure. The aforementioned degassing device can be an existing conventional device.

[0016] Preferably, the above-mentioned high-efficiency in vitro incubation device for crayfish also includes a frame, with the incubator located above the frame and the first water tank and the second water tank located below the frame.

[0017] In this utility model of a high-efficiency in vitro hatching device for crayfish, the water temperature is controlled at 24-30℃, the dissolved oxygen content is maintained above 8mg / L, the pH is in the range of 7.0-8.5, the total hardness is 50-100mg / L, and the salinity is around 5%.

[0018] In this invention, the incubator, in conjunction with the inlet pipe flow rate, can control the uniform flow of shrimp eggs, simulating the incubation process of eggs in the mother's foot. Furthermore, the shrimp eggs do not stick together in the incubation tank. Based on density differences, if dead eggs occur during incubation, they will float to the upper layer of the incubator and flow with the water to the outlet pipe, where they can be easily removed manually. In traditional in vitro incubation processes, live and dead shrimp eggs easily mix, and mold growth from the dead eggs can spread to the live eggs, leading to their death. This invention effectively avoids the difficulty of separating live and dead eggs.

[0019] This utility model relates to a highly efficient in vitro incubation device for crayfish, which solves the problems of inefficiency and bacterial contamination in conventional in vitro incubators, resulting in low egg survival rates. The device uses a water pump to evenly supply water into the incubator, causing the eggs to be agitated and mimicking the incubation process within the mother crayfish. A nano-bubble generator further increases the dissolved oxygen level in the water. Adjusting the water inlet allows for precise control of the incubator's water flow, ensuring the eggs float without being washed out. Simultaneously, the incubator is connected to a water collection channel. The water flows into a first tank for temporary storage and is then sterilized by a combination of ultraviolet lamps, an ozone generator, and a nano-bubble generator before flowing into a second tank. The second tank is powered by a water pump connected to the incubator's inlet, completing the integrated sterilization and oxygenation system.

[0020] This novel high-efficiency in vitro incubation device for crayfish ensures that eggs hatch under optimal conditions. Furthermore, by increasing the number of incubators on the support frame, the device can be utilized more efficiently, effectively overcoming the inefficiency of traditional incubators. Simultaneously, it employs conventional ultraviolet sterilization, combined with a nano-bubble generator and ozone generator for more thorough sterilization, overcoming the problem of mold growth in the water of conventional incubators. In addition, a gentle water flow is used to agitate the eggs, simulating the incubation process on the crayfish's abdomen, thus preventing the eggs from dying due to mechanical impact.

[0021] Compared with the prior art, the advantages of this utility model are: 1. The crayfish high-efficiency in vitro hatching device of this utility model adopts a water sterilization component, which can kill bacteria and other harmful substances in the water, which is conducive to the hatching of crayfish eggs.

[0022] 2. The crayfish high-efficiency in vitro incubation device of this utility model adopts a nano bubble machine. The nano bubble machine can evenly spray air into the water, thereby allowing oxygen to diffuse into the water and providing a continuous supply of oxygen, which is conducive to the hatching of crayfish eggs.

[0023] 3. This utility model's high-efficiency in vitro incubation device for crayfish can simulate the natural incubation process, removing fertilized eggs from the mother for incubation under more controlled conditions. This device improves survival rates by detaching and disinfecting the eggs from the crayfish's abdomen and placing them in the incubator. This ensures the eggs are evenly and subtly exposed to water, providing ample oxygen for development at all times. Furthermore, during development, the device can separate dead and live eggs, effectively reducing the rate of fungal infection. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the structure of the high-efficiency in vitro incubation device for crayfish, as shown in the embodiment.

[0026] Figure 2 This is a schematic diagram of the incubator in an embodiment.

[0027] Legend 1. Drain pipe; 2. Water collection chute; 3. Ultraviolet lamp; 4. Ozone generator; 5. Mesh screen; 6. First water tank; 7. Degassing instrument; 8. Nano bubble machine; 9. Water inlet pipe; 901. Branch pipe; 902. Valve; 10. Frame; 11. Second water tank; 12. Incubator; 1201. Main cylinder; 1202. Upper edge trough; 14. Overflow outlet pipe. Detailed Implementation

[0028] To facilitate understanding of this utility model, it will be described more comprehensively and in detail below with reference to the accompanying drawings and preferred embodiments. However, the scope of protection of this utility model is not limited to the following specific embodiments.

[0029] It should be noted that when a component is described as being "fixed to, attached to, connected to or connected to" another component, it can be directly fixed to, attached to, connected to or connected to the other component, or it can be indirectly fixed to, attached to, connected to or connected to the other component through other intermediate connectors.

[0030] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of protection of this invention.

[0031] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0032] Example: like Figure 1 As shown, the high-efficiency in vitro crayfish hatching device of this embodiment includes an incubator 12, a first water tank 6, and a second water tank 11. The incubator 12 is connected to the first water tank 6 via an overflow outlet pipe 14. The first water tank 6 is connected to the second water tank 11 via a nanobubble generator 8 for increasing the oxygen content in the water. The second water tank 11 is connected to the incubator 12 via an inlet pipe 9. The first water tank 6 is equipped with a water sterilization component. The inlet of the nanobubble generator 8 is connected to the first water tank 6, and the outlet of the nanobubble generator 8 is connected to the second water tank 11.

[0033] In this embodiment, multiple incubators 12 are arranged side by side (such as...). Figure 1 (As shown in the figure, four are shown). The water inlet pipe 9 is provided with multiple branch pipes 901, and each branch pipe 901 is equipped with a valve 902.

[0034] In this embodiment, a water collection slide 2 is also included. The overflow outlet pipes 14 of multiple incubators 12 are connected to the water collection slide 2. The water collection slide 2 is connected to the first water tank 6 through a water pipe 1.

[0035] In this embodiment, the first water tank 6 is equipped with a screen 5, and the outlet of the drain pipe 1 is located in the screen 5.

[0036] In this embodiment, the outlet of the branch pipe 901 extends downward to the lower part of the incubator 12.

[0037] like Figure 2 As shown, in this embodiment, the incubator 12 includes a main body cylinder 1201 and an upper edge groove 1202. The upper edge groove 1202 surrounds the upper part of the main body cylinder 1201. The top of the main body cylinder 1201 and the top of the upper edge groove 1202 are flush. The bottom of the main body cylinder 1201 is arc-shaped. The overflow water pipe 14 is located at the bottom of the upper edge groove 1202.

[0038] In this embodiment, the water sterilization component includes an ultraviolet lamp 3 installed in the first water tank 6, and a switch can be installed on the ultraviolet lamp 3.

[0039] In this embodiment, the water sterilization component includes an ozone generator 4 for providing ozone at regular intervals. The outlet of the ozone generator 4 is connected to the inlet of the nanobubble generator 8. The ozone generator 4 can be equipped with an ozone sensor to prevent ozone levels from exceeding the standard.

[0040] In this embodiment, the rated power of the ultraviolet lamp 3 is 5W, the power of the nano bubble machine 8 is 650W, and the flow rate is 400L / h.

[0041] In this embodiment, a siphon connecting pipe is provided between the first water tank 6 and the second water tank 11, and a degassing instrument 7 is connected to the siphon connecting pipe.

[0042] In this embodiment, a frame 10 is also included, an incubator 12 is located above the frame 10, and a first water tank 6 and a second water tank 11 are located below the frame 10.

[0043] In this embodiment of the high-efficiency in vitro incubation device for crayfish, several eggs to be incubated are placed in the incubator 12 during use. The incubator 12 has an inlet pipe 9 to provide water flow, and there is an overflow outlet pipe 14 on the side of the incubator 12. The overflow outlet pipe 14 is connected to the water collection slide 2 to discharge excess water into the water collection slide 2. The water collection slide 2 is connected to the first water tank 6. The first water tank 6 and the second water tank 11 are connected through a nano bubble machine 8. The nano bubble machine 8 is connected to an ozone generator 4. A water pump is placed in the second water tank 11, and the inlet pipe 9 is connected to the outlet of the water pump.

[0044] After the device is connected, add water to the first water tank 6 and the second water tank 11 to about 50% of their volume before starting the trial. Note that when using the ozone generator 4 with the nano-bubble machine 8 for sterilization, be sure to open the windows for ventilation to prevent long-term poisoning in the ozone environment. After putting in the shrimp eggs, adjust the water flow rate of the inlet pipe 9 so that the eggs neither escape from the incubator 12 nor settle at the bottom.

[0045] After the equipment is debugged, hatching can begin normally. During the hatching period, dead eggs should be removed regularly. After hatching, the hatching rate (hatched juveniles / total eggs × 100%) should be calculated. After the juveniles can swim freely (12 days later), the hatching rate (free-swimming juveniles / total eggs × 100%) should be calculated.

[0046] In this embodiment, 230 eggs were introduced on November 20, 2024, and hatching ended on December 18, 2024. A total of 169 eggs hatched, and 160 eggs hatched. The egg hatching rate was 73.47%, and the hatching rate was 69.57%. Compared with the natural conventional hatching rate of about 20% and hatching rate of about 10%, the hatching rate and hatching rate of this embodiment are significantly higher.

Claims

1. A highly efficient in vitro incubation device for crayfish, characterized in that, The device includes an incubator (12), a first water tank (6), and a second water tank (11). The incubator (12) is connected to the first water tank (6) via an overflow pipe (14). The first water tank (6) is connected to the second water tank (11) via a nanobubble machine (8) for increasing the oxygen content in the water. The second water tank (11) is connected to the incubator (12) via an inlet pipe (9). The first water tank (6) is equipped with a water sterilization component.

2. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, The incubators (12) are arranged in a row, and the water inlet pipe (9) is provided with a number of branch pipes (901), and each branch pipe (901) is equipped with a valve (902).

3. The high-efficiency in vitro incubation device for crayfish according to claim 2, characterized in that, It also includes a water collection slide (2), and the overflow outlet pipes (14) of the plurality of incubators (12) are connected to the water collection slide (2), and the water collection slide (2) is connected to the first water tank (6) through a water pipe (1).

4. The high-efficiency in vitro incubation device for crayfish according to claim 3, characterized in that, The first water tank (6) is equipped with a screen (5), and the outlet of the drain pipe (1) is located in the screen (5).

5. The high-efficiency in vitro incubation device for crayfish according to claim 2, characterized in that, The outlet of the branch pipe (901) extends downward to the lower part of the incubator (12).

6. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, The incubator (12) includes a main body cylinder (1201) and an upper edge groove (1202). The upper edge groove (1202) surrounds the upper body cylinder (1201). The top of the main body cylinder (1201) and the top of the upper edge groove (1202) are flush. The bottom of the main body cylinder (1201) is arc-shaped. The overflow outlet pipe (14) is located at the bottom of the upper edge groove (1202).

7. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, The water sterilization component includes an ultraviolet lamp (3) installed in the first water tank (6).

8. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, The water sterilization component includes an ozone generator (4) for providing ozone at regular intervals, the outlet of the ozone generator (4) being connected to the inlet of the nanobubble machine (8).

9. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, A siphon connecting pipe is provided between the first water tank (6) and the second water tank (11), and a degassing instrument (7) is connected to the siphon connecting pipe.

10. The high-efficiency in vitro incubation device for crayfish according to claim 1, characterized in that, It also includes a frame (10), the incubator (12) is located above the frame (10), and the first water tank (6) and the second water tank (11) are located below the frame (10).