Embryo freezing console

Abstract

The utility model discloses an embryo freezing operation box belongs to biomedical equipment technical field, including the freezing box for providing stable embryo freezing environment, the side surface intercommunication of freezing box has the pressure relief pipe for high pressure gas circulation, the one end fixed mounting of pressure relief pipe is away from freezing box has the ring column, the inside of ring column is provided with the multiple circle guide groove for limiting displacement direction, the inside connection of multiple circle guide groove has a plurality of springs for providing the spring of tension, a plurality of the end of spring is connected with multiple circle moving disc, multiple circle moving disc slidingly arranged in the inside of multiple circle guide groove, this embryo freezing operation box realizes efficient pressure relief through mechanical linkage, avoids the situation that the overpressure in the box is difficult to discharge, prevents the damage of the sealing element of the box body, avoids the overpressure interference air current and temperature stability in freezing box simultaneously, reduces the risk of embryo stress, guarantees the operation safety and embryo activity.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical equipment technology, and in particular to an embryo cryopreservation operation box. Background Technology

[0002] Embryo freezing is a crucial step in assisted reproductive technology, aiming to preserve embryos for subsequent thawing and transfer. Embryos are extremely sensitive to environmental factors such as temperature, humidity, cleanliness, and external vibrations. Instability in any environmental factor during embryo handling before freezing, the addition of freezing solution, and the transfer of embryos to the freezing carrier can affect embryo quality and viability. Weak one-way pressure relief can lead to difficulty in releasing overpressure within the incubator, potentially damaging the incubator's seals, interfering with airflow and temperature stability, increasing the risk of embryo stress, and affecting operational safety and embryo viability.

[0003] A search of Chinese patent documents (authorization announcement number CN222815149U) reveals that this utility model provides an embryo cryopreservation operation box, comprising a box body assembled together, a metal inner liner disposed within the box body, and a top cover installed on the upper part of the box body. The box body includes a bottom mold base and a top module, with several sets of intermediate modules stackable between the bottom mold base and the top module to adjust the height of the box body according to the metal inner liner. A lighting component is provided on the top of the box body, which is detachably installed on the top module via a magnetic connecting post. Personnel can adjust the height and angle of the lamp holder through an adjusting component to allow the light source to illuminate the metal inner liner, facilitating observation and operation. This device can meet basic usage requirements; however, its weak one-way pressure relief capacity can lead to difficulty in releasing overpressure inside the box, potentially damaging the box body seals, interfering with airflow and temperature stability, increasing the risk of embryo stress, and affecting operational safety and embryo viability. Utility Model Content

[0004] The purpose of this invention is to provide an embryo freezing operation box to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an embryo freezing operation box, comprising a freezing box for providing a stable embryo freezing environment, wherein a pressure relief pipe for high-pressure gas flow is connected to the side of the freezing box, and an annular column is fixedly installed at the end of the pressure relief pipe away from the freezing box; The annular column has a multi-circular guide groove inside to limit the direction of displacement. Multiple springs for providing tension are connected inside the multi-circular guide groove. The ends of the multiple springs are connected to a multi-circular movable disk. The multi-circular movable disk is slidably disposed inside the multi-circular guide groove. A connecting column for support is connected to the end of the multi-circular movable disk away from the spring. A connecting frame for providing installation space is fixedly connected to the end of the connecting column away from the multi-circular movable disk. A conical plug for preventing external gas from entering the pressure relief pipe is installed on the side of the connecting frame near the pressure relief pipe.

[0006] Preferably, the multi-circular guide groove is provided with multiple guide holes on the side near the connecting frame to limit the displacement of the connecting column, and the connecting column completely penetrates the interior of the guide holes.

[0007] Preferably, a conical gas guide block is fixedly installed inside the annular column to cooperate with the conical plug to prevent external gas from entering the pressure relief pipe, and multiple magnetic columns for attracting the conical plug are installed inside the conical gas guide block.

[0008] Preferably, the pressure relief pipe has an exhaust port at one end near the freezer, and a protective grille is installed inside the exhaust port to prevent impurities from entering the pressure relief pipe.

[0009] Preferably, the end of the annular column away from the pressure relief pipe is connected to an exhaust pipe for gas discharge, and the end of the exhaust pipe is connected to a connecting pipe for connection with an external purification mechanism.

[0010] Preferably, the top of the freezer is equipped with a sealed top cover for heat preservation, and an observation mirror for observing the inside of the freezer is installed inside the sealed top cover. Multiple temperature control tubes are installed on the vertical inner wall of the freezer.

[0011] Preferably, the inner bottom wall of the freezer is equipped with a refrigeration unit for providing refrigeration, and the interior of the freezer is equipped with a placement box for easy handling of frozen embryos.

[0012] Compared with the prior art, the technical effects and advantages of this utility model are as follows: This embryo cryopreservation chamber utilizes a highly efficient pressure relief and protection structure comprised of components such as a pressure relief pipe, annular column, conical plug, conical air guide block, spring, and magnetic column, effectively addressing the issue of weak one-way pressure relief capability. When the internal pressure of the cryopreservation chamber abnormally increases, high-pressure gas enters the annular column through the exhaust port of the pressure relief pipe (protective grilles block impurities), impacting the conical plug. When the gas pressure exceeds the sum of the attraction force of the magnetic column and the tension force of the spring, the conical plug separates from the conical air guide block, and the gas quickly escapes through the gap. After the pressure drops to a safe range, the spring and magnetic column together pull the conical plug back to its original sealing position. This structure achieves efficient pressure relief through mechanical linkage, avoiding situations where overpressure within the chamber is difficult to expel, preventing damage to chamber seals (such as the sealing structure of the top cover), and preventing overpressure from interfering with airflow and temperature stability within the cryopreservation chamber (ensuring the temperature control effect of the temperature control pipe and refrigeration unit), reducing the risk of embryo stress, and ensuring operational safety and embryo viability.

[0013] This embryo freezing chamber, thanks to its stable temperature control and operational protection structure comprised of a freezing chamber, sealed top cover, observation mirror, cooling unit, temperature control tubes, and placement box, provides an excellent environment for embryo freezing. The sealed top cover enhances insulation, reduces heat loss, and, combined with the observation mirror, allows real-time monitoring of the internal conditions, preventing disruption of the temperature environment by opening the top cover. The cooling unit rapidly lowers the temperature, and multiple evenly distributed temperature control tubes precisely regulate the temperature, ensuring a stable low temperature within the freezing chamber. The placement box provides an independent area for embryos and operating tools, facilitating organized operation. This structure continuously maintains the stable low-temperature environment required by the embryos, reducing interference from external factors, while allowing operators to monitor the operation progress without disrupting the internal environment, improving the safety and convenience of embryo handling, and further protecting embryo viability. Attached Figure Description

[0014] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a cross-sectional view of the present invention; Figure 3 This is a schematic diagram of the internal structure of this utility model; Figure 4 This utility model Figure 2 Enlarged view of point A in the middle; Figure 5 This utility model Figure 3 Enlarged view of point B in the middle.

[0016] Explanation of reference numerals in the attached figures: In the diagram: 1. Freezer; 101. Sealed top cover; 102. Observation mirror; 103. Refrigeration unit; 104. Placement box; 105. Temperature control tube; 2. Pressure relief pipe; 201. Exhaust port; 202. Protective grille; 203. Conical air guide block; 204. Magnetic column; 3. Annular column; 301. Multi-circle guide groove; 302. Spring; 303. Multi-circle moving plate; 304. Connecting column; 305. Connecting frame; 306. Conical block; 307. Guide hole; 4. Exhaust pipe; 401. Connecting pipe. Detailed Implementation

[0017] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.

[0018] The connection method can be any existing method, such as bonding, welding, or bolting, depending on the actual needs.

[0019] like Figures 1 to 5 An embryo freezing operation box is shown, including a freezing box 1 for providing a stable embryo freezing environment. A pressure relief pipe 2 for high-pressure gas flow is connected to the side of the freezing box 1. An annular column 3 is fixedly installed at the end of the pressure relief pipe 2 away from the freezing box 1. The annular column 3 has a multi-circular guide groove 301 inside to limit the direction of displacement. Multiple springs 302 for providing tension are connected inside the multi-circular guide groove 301. The ends of the multiple springs 302 are connected to multi-circular moving disks 303. The multi-circular moving disks 303 are slidably disposed inside the multi-circular guide groove 301. A connecting column 304 for support is connected to the end of the multi-circular moving disk 303 away from the springs 302. A connecting bracket 305 for providing installation space is fixedly connected to the end of the connecting column 304 away from the multi-circular moving disk 303. A conical plug 306 for preventing external gas from entering the pressure relief pipe 2 is installed on the side of the connecting bracket 305 near the pressure relief pipe 2.

[0020] The multi-circle guide groove 301 is provided with multiple guide holes 307 on the side near the connecting frame 305 to limit the displacement of the connecting column 304. The connecting column 304 completely penetrates the interior of the guide hole 307. The annular column 3 is fixedly installed with a conical air guide block 203 to cooperate with the conical block 306 to prevent external gas from entering the pressure relief pipe 2. The conical air guide block 203 is installed with multiple magnetic columns 204 to attract the conical block 306. High-pressure gas flows along the pressure relief pipe 2 to the annular column 3 and impacts the conical block 306 inside the annular column 3. When the gas pressure exceeds the sum of the attraction force of the magnetic column 204 on the conical block 306 and the tension of the spring 302, the conical block 306 separates from the conical air guide block 203. The gas enters the side of the annular column 3 away from the pressure relief pipe 2 through the gap between the conical air guide block 203 and the conical block 306. During this process, the conical block 306 drives the connecting column 304 to move through the connecting frame 305. The connecting column 304 slides along the guide hole 307 in the multi-circle guide groove 301. The multi-circle moving disk 303 slides with the connecting column 304 in the multi-circle guide groove 301 and stretches multiple springs 302. When the internal air pressure of the freezer 1 drops to a safe range, the tension of the spring 302 and the attraction of the magnetic column 204 on the conical block 306 work together to pull the conical block 306 back to its original position, re-fitting with the conical air guide block 203, closing the gas flow channel and preventing external gas from entering the pressure relief pipe 2.

[0021] The pressure relief pipe 2 is provided with an exhaust port 201 at one end near the freezer 1. The exhaust port 201 is equipped with a protective grid 202 to prevent impurities from entering the pressure relief pipe 2. When the internal air pressure of the freezer 1 rises abnormally, the high-pressure gas enters the pressure relief pipe 2 through the exhaust port 201 at the end near the freezer 1. The protective grid 202 inside the exhaust port 201 can block impurities in the freezer 1 from entering the pressure relief pipe 2, preventing pipe blockage. The end of the annular column 3 away from the pressure relief pipe 2 is connected to an exhaust pipe 4 for gas discharge. The end of the exhaust pipe 4 is connected to a connecting pipe 401 for connection with an external purification mechanism. During the air pressure regulation process, the gas passing through the gap between the conical air guide block 203 and the conical block 306 enters the end of the annular column 3 away from the pressure relief pipe 2, flows along the exhaust pipe 4 connected to the annular column 3, and is finally discharged through the connecting pipe 401 at the end of the exhaust pipe 4. The connecting pipe 401 can be connected to an external purification mechanism, so that the exhaust gas is purified before entering the external environment, avoiding the spread of pollutants that may be carried in the gas; at the same time, the multi-circle guide groove 301 of the annular column 3 restricts the displacement direction of the multi-circle moving disk 303, ensuring that the connecting column 304 drives the conical block 306 to move stably, preventing sealing failure or poor pressure relief caused by component misalignment, ensuring the reliability of the entire air pressure regulation process, and thus providing a stable and safe operating environment for the embryos inside the freezer 1.

[0022] The top of the freezer 1 is equipped with a sealed top cover 101 for heat preservation. Inside the sealed top cover 101 is an observation mirror 102 for observing the inside of the freezer 1. Multiple temperature control tubes 105 are installed on the vertical inner wall of the freezer 1. A cooling unit 103 for providing cooling is installed on the inner bottom wall of the freezer 1. Inside the freezer 1 is a placement box 104 for easy handling of frozen embryos. The freezer 1 provides a core operating space for embryo freezing. The sealed top cover 101 enhances the heat preservation effect and prevents heat loss inside the box. The operator can observe the inside of the freezer 1 in real time through the observation mirror 102 inside the sealed top cover 101 and monitor the progress of embryo handling without opening the top cover. After the cooling unit 103 on the bottom wall of the freezer 1 is activated, it can quickly reduce the temperature inside the freezer. At the same time, multiple temperature control tubes 105 on the vertical inner wall work together to achieve precise temperature control inside the freezer through the evenly distributed temperature control tubes 105, ensuring that the freezer 1 maintains the stable low temperature environment required for embryo freezing. Embryos and related operating tools are placed in the placement box 104 inside the freezer 1. The placement box 104 provides an independent operating area for embryos, which facilitates the operation of adding freezing solution and transferring embryos in an orderly manner, avoiding external interference to the embryos. The cooling unit 103, as the core cooling component of the freezer 1, is mainly composed of a semiconductor cooling chip, a cooling fan, a thermally conductive aluminum block, and an insulation shell. The semiconductor cooling chip is the core cooling element. It achieves heat transfer through the Peltier effect of the semiconductor material after being energized. Its cold end is attached to the heat-conducting aluminum block to quickly conduct the cold energy into the freezer 1. The cooling fan is installed at the hot end of the semiconductor cooling chip and works with the heat sink to exhaust the generated heat to the outside of the freezer 1, so as to avoid the hot end temperature from being too high and affecting the cooling efficiency. The heat insulation shell is made of polyurethane insulation material and is wrapped around the outside of the cooling part 103 to reduce the loss of cold energy and ensure stable cooling efficiency. The temperature control tube 105 is used to assist in temperature control and temperature uniformity. It consists of a metal heat pipe, a temperature sensor, and a heating wire (spare). The copper-based heat pipes are evenly distributed along the vertical inner wall of the freezer 1, rapidly conducting the cooling energy generated by the refrigeration unit 103 to ensure uniform temperature distribution within the freezer. A temperature sensor is embedded inside the temperature control tube 105, collecting the tube temperature in real time and transmitting it to the equipment control system for comparison with the set temperature. A spare heating wire is wound around the outside of the heat pipes. When the local temperature inside the freezer is too low, the control system can activate the heating wire to fine-tune the temperature, preventing excessive temperature fluctuations. During operation, after the semiconductor cooling chip of the refrigeration unit 103 is energized, the cold end releases cooling energy into the freezer 1 through the heat-conducting aluminum block, while the cooling fan simultaneously starts to dissipate heat from the hot end. The temperature sensor on the temperature control tube 105 monitors the temperature inside the freezer in real time. If the temperature is higher than the set value, the control system increases the cooling power of the refrigeration unit 103. If the local temperature is uneven, the temperature control tube 105 rapidly conducts cooling energy through the heat pipes to balance the temperature inside the freezer. If the temperature is lower than the threshold, the spare heating wire is activated for fine-tuning, ultimately stabilizing the temperature inside the freezer 1 within the range required for embryo freezing, ensuring precise and controllable environmental temperature for the embryos.

[0023] To ensure the stable functioning of the embryo cryopreservation chamber, it is necessary to address the impact of various factors on normal operation from six core dimensions: environmental adaptation, maintenance, sealing and insulation, material selection, component compatibility, and personnel operation. Specific measures are as follows: Regarding environmental adaptation, a stable external environment must be provided for the equipment to avoid interference from environmental factors. First, place the cryopreservation chamber 1 in a well-ventilated area of ​​the laboratory, away from direct sunlight and heat sources (such as air conditioning vents and heating equipment) to prevent external high temperatures from affecting the cooling efficiency of the refrigeration unit 103 and the temperature control accuracy of the temperature control tube 105, ensuring that the temperature inside the cryopreservation chamber 1 remains stable within the required range for embryos. Second, avoid placing the equipment in environments with frequent vibrations (such as near centrifuges or vacuum pumps) to prevent long-term vibration from increasing the clearance between the multi-circular guide groove 301 and the multi-circular moving disk 303, or loosening the connection between the pressure relief pipe 2 and the annular column 3, affecting the sealing performance and pressure relief efficiency of the pressure relief structure. At the same time, the humidity of the laboratory environment should be controlled between 40% and 60% to avoid high humidity causing the magnetic column 204 to rust and fail, or causing mold to grow on the surface of the protective grid 202 and block the exhaust port 201, so as to ensure the normal operation of all components of the equipment. In terms of maintenance, a regular maintenance mechanism needs to be established to extend component life and prevent malfunctions. For the pressure relief structure, the connection between the exhaust pipe 4 and the connecting pipe 401 should be removed every 3 months, and the dust and impurities inside the exhaust pipe 4 should be cleaned to prevent pipe blockage from affecting gas discharge; at the same time, the elasticity of the spring 302 should be checked. If it is found that the spring 302 cannot return to its original position after being stretched, it should be replaced in time to ensure that it can provide sufficient pulling force for the reset of the conical block 306. For temperature control and sealing components, the surface of the observation mirror 102 should be wiped with 75% medical alcohol every month to remove stains and ensure clear observation to avoid affecting operation due to blurred vision; the sealing strip of the sealing top cover 101 should be checked every 6 months. If it is aged and cracked, it should be replaced in time to enhance the heat preservation effect and prevent temperature loss in the freezer 1; at the same time, the linkage accuracy between the temperature control tube 105 and the refrigeration unit 103 should be calibrated to ensure that the temperature fluctuation does not exceed ±0.5℃. In addition, the annular column 3 is disassembled every 12 months to clean the grease residue inside the multi-circle guide groove 301 and guide hole 307, and medical-grade grease is reapplied to reduce frictional wear when the multi-circle moving disk 303 and connecting column 304 slide, ensuring smooth component fit. Regarding sealing and insulation, the sealing design and insulation measures of key components need to be strengthened. For freezer 1, in addition to ensuring the integrity of the sealing strip of the sealing top cover 101, a magnetic strip is added to the contact edge between the sealing top cover 101 and freezer 1 to enhance the sealing performance after closure and reduce cold air leakage. At the same time, a layer of insulation cotton with a thickness of not less than 5mm is wrapped around the outer wall of freezer 1 to further reduce the impact of external temperature on the internal environment and reduce the workload of the refrigeration unit 103. For the pressure relief structure, sealing tape is wrapped around the connection between the pressure relief pipe 2 and freezer 1, and an O-ring is embedded in the threaded connection between the annular column 3 and the pressure relief pipe 2 to prevent high-pressure gas from leaking during the flow process and ensure pressure relief efficiency. At the same time, a wear-resistant silicone pad is pasted on the sealing surface of the conical block 306 to enhance its sealing performance when it is in contact with the conical air guide block 203, preventing external gas from entering the pressure relief pipe 2 through the gap and contaminating the internal environment of freezer 1. In terms of material selection, priority should be given to materials that are suitable for biomedical applications and have stable performance. The inner liner and placement box 104 of the freezer 1 are made of medical-grade 304 stainless steel. This material is corrosion-resistant and easy to clean, preventing contamination of the embryos due to rust or release of harmful substances during embryo handling. The multi-circular moving tray 303 and connecting column 304 are made of high-strength polyetheretherketone (PEEK) material, which has excellent wear resistance and can reduce wear when the multi-circular guide groove 301 and connecting column 304 slide, extending the service life of the components. The magnetic column 204 uses neodymium iron boron strong magnetic material and is chrome-plated for rust prevention, ensuring that it has sufficient attraction for a long time and preventing the conical block 306 from failing to fit tightly against the conical air guide block 203 due to weakened magnetism. The protective grid 202 is made of 316L stainless steel, which has better corrosion resistance than 304 stainless steel and can prevent the grid from rusting and clogging the exhaust port 201 due to refrigerant residue, ensuring smooth gas flow. For component fit dimensions, the structural design needs to be optimized to ensure that all components work together. The machining accuracy of the inner wall of the multi-circle guide groove 301 is controlled within ±0.02mm, and the fit clearance with the multi-circle moving disk 303 is maintained between 0.05-0.1mm. This ensures that the multi-circle moving disk 303 can slide flexibly while avoiding excessive clearance that could lead to gas leakage. At the same time, the deviation between the axis of the guide hole 307 and the axis of the connecting column 304 is controlled within 0.03mm to prevent the connecting column 304 from getting stuck when sliding, which would affect the accurate repositioning of the conical block 306. For the temperature control system, the temperature control tubes 105 are evenly distributed on the vertical inner wall of the freezer 1, with the distance between adjacent temperature control tubes 105 not exceeding 15cm. This ensures uniform temperature inside the freezer and avoids localized excessively high or low temperatures that could affect embryo viability. At the same time, the heat dissipation vents of the refrigeration unit 103 are oriented away from the freezer 1 to prevent heat generated during the cooling process from flowing back into the freezer 1 and interfering with the temperature control effect. Regarding personnel operation, standardized operating procedures are necessary to avoid human error. Before starting the equipment, operators must check whether the connecting pipe 401 is correctly connected to the external purification mechanism to prevent unpurified gas from being directly emitted and causing pollution. During operation, when reaching into the freezing chamber 1 to process embryos, avoid forcefully bumping the placement box 104 or the temperature control tube 105 to prevent component displacement and affect equipment stability. When adjusting the sealing top cover 101, ensure that the top cover is completely closed before starting the refrigeration unit 103 to avoid temperature loss due to starting the equipment with the cover open. When dealing with abnormal pressure relief, the refrigeration unit 103 must be shut down first. After the gas pressure inside the freezing chamber 1 drops to the normal range, check the pressure relief structure. Do not disassemble the annular column 3 or the exhaust pipe 4 under high pressure to prevent injury or damage to components caused by high-pressure gas impact. At the same time, operators should be trained regularly to familiarize themselves with the functions of each component and operating precautions, reduce equipment failures caused by improper operation, and ensure the continuous and stable operation of the embryo freezing chamber.

[0024] Working principle In use, the embryo freezing chamber 1 provides the core operating space. Its sealed top cover 101 enhances insulation, preventing heat loss. Operators can monitor the internal conditions in real-time through an observation mirror 102 inside the sealed top cover 101, allowing them to track embryo handling progress without opening the cover. The cooling unit 103 on the bottom wall of the freezing chamber 1 activates for rapid cooling. Multiple temperature control tubes 105 on the vertical inner wall work together, achieving precise temperature control through even distribution, maintaining a stable low-temperature environment required for embryo freezing. Embryos and operating tools are placed in the internal placement box 104, providing an independent operating area for embryos, facilitating orderly addition of freezing solution, embryo transfer, and other operations, preventing embryo interference. When the internal pressure of the freezing chamber 1 abnormally increases, high-pressure gas enters the pressure relief pipe 2 through the exhaust port 201 near one end of the freezing chamber 1. A protective grille 202 inside the exhaust port 201 prevents impurities from entering and causing blockages. High-pressure gas flows along the pressure relief pipe 2 to the annular column 3 and impacts the internal conical block 306. When the gas pressure exceeds the sum of the attraction force of the magnetic column 204 on the conical block 306 and the tension of the spring 302, the conical block 306 separates from the conical gas guide block 203. Gas enters the annular column 3 away from the pressure relief pipe 2 through the gap between them. At the same time, the conical block 306 drives the connecting column 304 to slide along the guide hole 307 in the multi-circle guide groove 301 via the connecting bracket 305. The multi-circle moving disk 303 slides with the connecting column 304 and stretches the spring 302. When the gas pressure inside the freezer 1 drops to a safe range, the tension of the spring 302 and the attraction force of the magnetic column 204 work together to reset the conical block 306, closing the gas passage and preventing external gas from entering. The gas passing through the gap flows along the exhaust pipe 4 connected to the annular column 3 and is discharged through the connecting pipe 401 at the end of the exhaust pipe 4. The connecting pipe 401 can be connected to an external purification mechanism to purify the gas before discharge, thus preventing the spread of pollutants. At the same time, the multi-circle guide groove 301 of the annular column 3 restricts the displacement direction of the multi-circle moving disk 303, ensuring the stable movement of the conical block 306, ensuring reliable gas pressure regulation, and providing a stable and safe environment for the embryo.

[0025] It should be noted that in this article, relational terms such as one and two are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations.

[0026] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An embryo cryopreservation chamber, comprising a cryochamber (1) for providing a stable embryo cryopreservation environment, characterized in that: The side of the freezer (1) is connected to a pressure relief pipe (2) for high-pressure gas flow, and an annular column (3) is fixedly installed at the end of the pressure relief pipe (2) away from the freezer (1). The annular column (3) is provided with a multi-circular guide groove (301) for limiting the displacement direction. Multiple springs (302) for providing tension are connected inside the multi-circular guide groove (301). The ends of the multiple springs (302) are connected to a multi-circular moving disk (303). The multi-circular moving disk (303) is slidably disposed inside the multi-circular guide groove (301). A connecting column (304) for support is connected to the end of the multi-circular moving disk (303) away from the springs (302). A connecting frame (305) for providing installation space is fixedly connected to the end of the connecting column (304) away from the multi-circular moving disk (303). A conical plug (306) for preventing external gas from entering the pressure relief pipe (2) is installed on the side of the connecting frame (305) near the pressure relief pipe (2).

2. The embryo freezing operation box according to claim 1, characterized in that: The multi-circle guide groove (301) is provided with a plurality of guide holes (307) on the side near the connecting frame (305) for limiting the displacement of the connecting column (304), and the connecting column (304) completely penetrates the interior of the guide hole (307).

3. The embryo freezing operation box according to claim 1, characterized in that: The annular column (3) is fixedly installed with a conical gas guide block (203) for cooperating with the conical block (306) to prevent external gas from entering the pressure relief pipe (2). The conical gas guide block (203) is installed with multiple magnetic columns (204) for attracting the conical block (306).

4. The embryo freezing operation box according to claim 1, characterized in that: The pressure relief pipe (2) is provided with an exhaust port (201) at one end near the freezer (1), and a protective grille (202) is installed inside the exhaust port (201) to prevent impurities from entering the pressure relief pipe (2).

5. The embryo freezing operation box according to claim 1, characterized in that: The end of the annular column (3) away from the pressure relief pipe (2) is connected to an exhaust pipe (4) for gas discharge, and the end of the exhaust pipe (4) is connected to a connecting pipe (401) for connection with an external purification mechanism.

6. The embryo freezing operation box according to claim 1, characterized in that: The freezer (1) is equipped with a sealed top cover (101) for heat preservation. An observation mirror (102) for observing the inside of the freezer (1) is installed inside the sealed top cover (101). Multiple temperature control tubes (105) are installed on the vertical inner wall of the freezer (1).

7. An embryo freezing operation box according to claim 6, characterized in that: The inner bottom wall of the freezer (1) is equipped with a refrigeration unit (103) for providing refrigeration, and the interior of the freezer (1) is equipped with a placement box (104) for facilitating the handling of frozen embryos.

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