A porous bone tissue material structure promoting vascularization

Abstract

The utility model discloses a kind of micro-porous bone tissue material structures of promoting vascularization, it is related to bone tissue engineering and regenerative medicine technical field, including the bone tissue main body made of biomaterial, bone tissue main body includes inner layer cortical bone, outer layer cortical bone and intermediate cancellous bone, intermediate cancellous bone is enclosed between inner layer cortical bone and outer layer cortical bone, micro-porous structure is equipped on inner layer cortical bone and outer layer cortical bone, and micro-porous structure includes the several micropores of multistage pore, solve the problem that current biological bone support cannot be effectively vascularized and osteogenesis when using.

Description

Technical Field

[0001] This utility model relates to the field of bone tissue engineering technology, specifically to a microporous bone tissue material structure that promotes vascularization. Background Technology

[0002] In the field of bone tissue engineering, bio-bone scaffolds are the core materials for repairing bone defects, and their performance directly determines the success or failure of bone repair and regeneration. An ideal bio-bone scaffold not only needs to provide stable physical support for bone tissue growth but also needs to effectively promote vascularization and osteogenic formation, thereby accelerating the bone healing process. However, current bio-bone scaffolds struggle to simultaneously meet these two key functional requirements in practical applications. Utility Model Content

[0003] Based on this, and in response to the above problems, this utility model proposes a microporous bone tissue material structure that promotes vascularization, thus solving the problem that current biological bone scaffolds cannot effectively vascularize and form bone during use.

[0004] The technical solution of this utility model is:

[0005] A microporous bone tissue material structure that promotes vascularization includes a bone tissue body made of biomaterial. The bone tissue body includes an inner cortical bone layer, an outer cortical bone layer, and an intermediate cancellous bone layer. The intermediate cancellous bone layer is wrapped between the inner and outer cortical bone layers. Both the inner and outer cortical bone layers have microporous structures, and the microporous structures include several micropores with multi-level pores.

[0006] Preferably, the shape of the main body of the bone tissue is a cube, cuboid, or sphere.

[0007] Preferably, when the shape of the main body of the bone tissue is a cube or cuboid, the length of the main body of the bone tissue ranges from 3cm to 20cm, the width ranges from 3cm to 20cm, and the thickness ranges from 0.1cm to 5cm.

[0008] Preferably, the cross-section of the micropores is circular or elliptical.

[0009] Preferably, the length of the long axis of the micropore ranges from 100 nm to 500 μm, the length of the short axis ranges from 100 nm to 500 μm, and the depth ranges from 100 nm to 20 mm.

[0010] Preferably, a number of micropores located on the inner cortical bone are vertically or obliquely disposed on the inner cortical bone, and a number of micropores located on the outer cortical bone are vertically or obliquely disposed on the outer cortical bone.

[0011] Preferably, the angle between the pore direction of the micropores located on the inner cortical bone and the inner cortical bone is in the range of 45° to 90°, and the angle between the pore direction of the micropores located on the outer cortical bone and the outer cortical bone is in the range of 45° to 90°.

[0012] Preferably, the total area of ​​the micropores on the inner cortical bone is 1% to 11% of the surface area of ​​the inner cortical bone, and the total area of ​​the micropores on the outer cortical bone is 1% to 11% of the surface area of ​​the outer cortical bone.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This invention utilizes biomaterials to create a bone tissue matrix whose structure mimics the three-layered structure of natural bone. Simultaneously, numerous micropores with multi-level voids are incorporated into the inner and outer cortical bone layers, constructing a three-dimensional channel network that promotes angiogenesis and osteogenic differentiation. This solves the problem of current biological bone scaffolds failing to effectively vascularize and form osteogenic structures during use. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of a microporous bone tissue material that promotes vascularization, as described in an embodiment of this utility model.

[0017] Figure 2 This is a partial cross-sectional view of a microporous bone tissue material structure that promotes vascularization, as described in this embodiment of the invention. Figure 1 ;

[0018] Figure 3 This is a partial cross-sectional view of a microporous bone tissue material structure that promotes vascularization, as described in this embodiment of the invention. Figure 2 ;

[0019] Explanation of reference numerals in the attached figures:

[0020] 1-Main bone tissue, 10-Inner cortical bone, 11-Outer cortical bone, 12-Intermediate cancellous bone, 13-Microporous structure, 100-Micropores. Detailed Implementation

[0021] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0022] In the description of the embodiments of this utility model, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model.

[0023] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0024] In this embodiment of the invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention according to the specific circumstances.

[0025] In this embodiment of the invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0026] The following disclosure provides many different implementations or examples for different structures of the embodiments of the present invention. To simplify the disclosure of the embodiments of the present invention, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the embodiments of the present invention. Furthermore, reference numerals and / or reference letters may be repeated in different examples of the embodiments of the present invention; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various implementations and / or arrangements discussed.

[0027] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0028] Example:

[0029] like Figures 1 to 3 As shown, this embodiment discloses a microporous bone tissue material structure that promotes vascularization, including a bone tissue body 1 made of biomaterial. The bone tissue body 1 includes an inner cortical bone 10, an outer cortical bone 11, and an intermediate cancellous bone 12. The intermediate cancellous bone 12 is wrapped between the inner cortical bone 10 and the outer cortical bone 11. Both the inner cortical bone 10 and the outer cortical bone 11 are provided with microporous structures 13, and the microporous structures 13 include a plurality of micropores 100 with multi-level pores.

[0030] This invention utilizes biomaterials to create a bone tissue body 1, the structure of which mimics the three-layer structure of natural bone. Simultaneously, a plurality of micropores 100 with multi-level porosity are formed on the inner cortical bone layer 10 and the outer cortical bone layer 11, constructing a three-dimensional channel network through these micropores, thereby promoting blood vessel ingrowth and osteogenic differentiation. This solves the problem that current biological bone scaffolds cannot effectively achieve vascularization and osteogenic differentiation during use.

[0031] Among them, the multi-level pores 100 refer to several micropores 100 of different sizes and depths.

[0032] It should be noted that the biomaterials used to construct the inner cortical bone 10 and the outer cortical bone 11 can be any biomaterials currently used to construct cortical bone, including but not limited to biometallic materials, bioceramic materials, biopolymer materials, and biocomposite materials made of two or more of the above materials, such as PLGA / β-TCP composite scaffolds and collagen-bioglass nanocomposite materials. The biomaterials used to construct the intermediate cancellous bone 12 can be any biomaterials currently used to construct cancellous bone, including but not limited to bioceramic materials, biopolymer materials, and biocomposite materials made of two or more of the above materials, such as ceramic-polymer composite scaffolds and nano-hydroxyapatite-collagen.

[0033] Among them, Figures 1 to 3Since the micro-orifice 100 is small and difficult to illustrate, only the same magnified aperture is used to indicate the existence of the micro-orifice 100. Figure 2 and Figure 3 The figure only shows the angle of the micropore 100; the size and shape of the micropore 100 are not shown in the figure. Figure 2 The diagram shown is a cross-sectional view of the structure when the micropores 100 are tilted. Figure 3 The diagram shows a cross-sectional view of the structure when the micropores 100 are vertically arranged.

[0034] To facilitate use in different parts, this embodiment is an improvement on the above embodiment. The difference from the above embodiment is that the shape of the bone tissue body 1 is a cube, cuboid, or sphere.

[0035] When the length of the bone tissue body 1 is equal to the width of the bone tissue body 1, the bone tissue body 1 is cubic in shape; when the length of the bone tissue body 1 is not equal to the width of the bone tissue body 1, the bone tissue body 1 is cuboid in shape.

[0036] When the shape of the bone tissue body 1 is a cube or cuboid, the length of the bone tissue body 1 ranges from 3cm to 20cm, the width ranges from 3cm to 20cm, and the thickness ranges from 0.1cm to 5cm.

[0037] The standardized size design facilitates industrial production and clinical operation. Different sizes of bone tissue body 1 can be selected according to the actual location to facilitate use in different locations.

[0038] To facilitate the promotion of vascularization and bone formation, this embodiment is an improvement on the above embodiment. The difference from the above embodiment is that the cross-section of the plurality of micropores 100 is circular or elliptical.

[0039] When the length of the major axis is equal to the length of the minor axis, the cross-section of several micro-holes 100 is circular; when the length of the major axis is not equal to the length of the minor axis, the cross-section of several micro-holes 100 is elliptical.

[0040] The length of the long axis of the micropore 100 ranges from 100nm to 500um, the length of the short axis ranges from 100nm to 500um, and the depth ranges from 100nm to 20mm.

[0041] A number of micropores 100 located on the inner cortical bone 10 are vertically or obliquely disposed on the inner cortical bone 10, and a number of micropores 100 located on the outer cortical bone 11 are vertically or obliquely disposed on the outer cortical bone 11.

[0042] The angle between the pore direction of the micropores 100 located on the inner cortical bone 10 and the inner cortical bone 10 ranges from 45° to 90°. The angle between the pore direction of the micropores 100 located on the outer cortical bone 11 and the outer cortical bone 11 ranges from 45° to 90°.

[0043] The total area of ​​the micropores 100 on the inner cortical bone 10 is 1% to 11% of the surface area of ​​the inner cortical bone 10, and the total area of ​​the micropores 100 on the outer cortical bone 11 is 1% to 11% of the surface area of ​​the outer cortical bone 11.

[0044] Micropore structure 13 is used to activate the HIF-1α signaling pathway, induce vascular endothelial growth factor (VEGF) secretion, and promote directional migration of endothelial cells and self-assembly of vascular lumens. Among them, micropore 100 with both long axis length and short axis length greater than 100 μm is used to allow vascular shoot extension, and micropore 100 with both long axis length and short axis length less than 10 μm is used to enhance capillary permeability and accelerate the diffusion of oxygen / nutrients.

[0045] By incorporating multiple micropores 100 with varying levels of porosity, vascularization and osteogenic processes can be facilitated. Micropores 100 with both long and short axes greater than 100 μm allow for vascular shoot extension, providing physical space for this extension and supporting the construction of a three-dimensional vascular network. Micropores 100 with both long and short axes less than 10 μm enhance protein adsorption and cell adhesion, promoting capillary permeability and substance exchange. The angular design of the micropores 100 optimizes cell migration pathways, promotes vascular branching, and simultaneously improves the material's mechanical properties.

[0046] The number of micropores 100 is calculated using the following formula:

[0047] N = (S * X) / M

[0048] Where N is the number of micropores 100, S is the surface area of ​​the inner cortical bone 10 or the outer cortical bone 11, X is the total area ratio of the micropores 100, and M is the area of ​​the micropores 100.

[0049] When the cross-section of micropore 100 is circular, the formula for the number of micropores 100 is as follows:

[0050]

[0051] Where d is the diameter of the micropore 100.

[0052] When the cross-section of micropore 100 is elliptical, the formula for the number of micropores 100 is as follows:

[0053] N = (S*X) / (x*a*b) where a is half the length of the major axis of the micro-orifice 100 and b is half the length of the minor axis of the micro-orifice 100.

[0054] To facilitate sterilization and maintain the pore morphology and bioactivity of the material, this embodiment is an improvement on the above embodiment. The difference from the above embodiment is that the bone tissue body 1 is sterilized by cobalt-60 gamma ray radiation with an absorbed dose of 25 kGy.

[0055] Sterilization by cobalt-60 gamma ray radiation can effectively kill microorganisms while avoiding the damage to the structure of biomaterials caused by high-temperature sterilization, thus maintaining the pore morphology and biological activity of the materials.

[0056] Working principle of this utility model:

[0057] This invention uses biomaterials to create a bone tissue body 1, the structure of which simulates the three-layer structure of natural bone. Simultaneously, a plurality of micropores 100 with multi-level pores are formed on the inner cortical bone 10 and the outer cortical bone 11, constructing a three-dimensional channel network through these micropores, thereby promoting blood vessel ingrowth and osteogenic differentiation.

[0058] Although preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the present invention.

[0059] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that any modifications, equivalent substitutions and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A microporous bone tissue material structure that promotes vascularization, comprising a bone tissue body (1) made of biomaterial, characterized in that, The main body of bone tissue (1) includes an inner cortical bone (10), an outer cortical bone (11) and an intermediate cancellous bone (12). The intermediate cancellous bone (12) is wrapped between the inner cortical bone (10) and the outer cortical bone (11). Both the inner cortical bone (10) and the outer cortical bone (11) are provided with microporous structures (13). The microporous structures (13) include several micropores (100) with multi-level pores.

2. The microporous bone tissue material structure for promoting vascularization according to claim 1, characterized in that, The main body of the bone tissue (1) is shaped like a cube, cuboid, or sphere.

3. The microporous bone tissue material structure for promoting vascularization according to claim 2, characterized in that, When the shape of the bone tissue body (1) is a cube or cuboid, the length of the bone tissue body (1) ranges from 3cm to 20cm, the width ranges from 3cm to 20cm, and the thickness ranges from 0.1cm to 5cm.

4. The microporous bone tissue material structure for promoting vascularization according to claim 3, characterized in that, The cross-section of several micropores (100) is circular or elliptical.

5. The microporous bone tissue material structure for promoting vascularization according to claim 4, characterized in that, The length range of the long axis of the micropore (100) is 100nm~500um, the length range of the short axis is 100nm~500um, and the depth range is 100nm~20mm.

6. The microporous bone tissue material structure for promoting vascularization according to claim 5, characterized in that, Several micropores (100) located on the inner cortical bone (10) are vertically or obliquely arranged on the inner cortical bone (10), and several micropores (100) located on the outer cortical bone (11) are vertically or obliquely arranged on the outer cortical bone (11).

7. The microporous bone tissue material structure for promoting vascularization according to claim 6, characterized in that, The angle between the pore direction of the micropores (100) on the inner cortical bone (10) and the inner cortical bone (10) is in the range of 45° to 90°. The angle between the pore direction of the micropores (100) on the outer cortical bone (11) and the outer cortical bone (11) is in the range of 45° to 90°.

8. The microporous bone tissue material structure for promoting vascularization according to claim 7, characterized in that, The total area of ​​several micropores (100) on the inner cortical bone (10) is 1% to 11% of the surface area of ​​the inner cortical bone (10), and the total area of ​​several micropores (100) on the outer cortical bone (11) is 1% to 11% of the surface area of ​​the outer cortical bone (11).

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