Solid phase extraction device

Abstract

The utility model relates to solid phase extraction technical field especially for solid phase extraction device, the top of base is fixedly connected with vacuum air chamber, the top of vacuum air chamber is installed with extraction mechanism, the inside of vacuum air chamber is provided with test tube support frame, the outside of vacuum air chamber is installed with adjustable air pump, the bottom of vacuum air chamber is provided with liquid discharge mechanism, the extraction mechanism includes sealed upper cover, the top of sealed upper cover is clamped with flow guide pipe, in the utility model, through the horizontal gap of 6-10cm between two rows of flow guide pipes, sufficient operation space is reserved for the operator to install / dismount extraction pipe, add sample, avoid the collision interference when many tubes are handled in parallel, the liquid storage tank of the bottom wall in the vacuum air chamber adopts slope edge design, can guide the quick convergence of waste liquid, cooperate with the liquid guide pipe of the liquid discharge adjusting pipe and telescopic flexible hose material outside the vacuum air chamber, can realize the directional discharge of waste liquid without extra placing waste liquid box.

Description

Technical Field

[0001] This utility model relates to the field of solid phase extraction technology, specifically to a solid phase extraction device. Background Technology

[0002] Solid phase extraction is a commonly used sample pretreatment technique, widely applied in fields such as environmental monitoring, food testing, and pharmaceutical analysis. As one of the core devices of this technique, the ease of use and processing efficiency of the manual solid phase extraction device directly affect the accuracy of experimental results and the experimental process. Currently, traditional manual solid-phase extraction devices have the following shortcomings. First, waste liquid collection is inconvenient, usually requiring an additional waste liquid container to collect the waste liquid generated during the extraction process. This not only increases the number of experimental steps but also makes it easy for waste liquid to leak due to improper placement of the waste liquid container, polluting the experimental environment. Second, the shell volume is small, and the gap design between the two rows of extraction columns is unreasonable, with too small a spacing. When multiple samples need to be processed simultaneously, operators are prone to collisions during the installation, disassembly of extraction columns, and sample addition, making operation inconvenient and seriously affecting the efficiency of processing multiple samples simultaneously. Furthermore, there is a lack of independent flow rate control components. A uniform flow control method is usually used, which cannot accurately control the flow rate according to the actual situation of the samples in different extraction columns, resulting in poor extraction effect for some samples and affecting the accuracy of experimental data. Therefore, a solid-phase extraction device is proposed to address the above problems. Utility Model Content

[0003] The purpose of this invention is to provide a solid-phase extraction apparatus to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: A solid-phase extraction apparatus includes a base, a vacuum chamber fixedly connected to the top of the base, an extraction mechanism installed on the top of the vacuum chamber, a test tube support frame installed inside the vacuum chamber, an adjustable air pump installed outside the vacuum chamber, and a draining mechanism installed at the bottom of the vacuum chamber. The extraction mechanism includes a sealed top cover, a guide tube is snapped onto the top of the sealed top cover, a flow regulating valve is fixedly connected to the top of the guide tube, and an extraction tube is snapped onto the top of the flow regulating valve. The draining mechanism includes a liquid storage tank, a drain regulating pipe is fixedly connected to the outside of the vacuum chamber, and a liquid guide pipe is fixedly connected to the outside of the drain regulating pipe.

[0005] As a further optimization of this utility model, the sealing cover is installed on the top of the vacuum chamber, and the guide tubes are evenly distributed inside the sealing cover.

[0006] As a further optimization of this utility model, the horizontal gap between the two rows of guide tubes is set to 6-10cm, and the test tube support frame is divided into upper and lower layers, which are connected by columns.

[0007] As a further optimization of this utility model, the bottom of the guide tube is located inside the vacuum chamber, the inside of the test tube support frame is provided with a snap-fit ​​groove, and the bottom of the guide tube is located directly above the snap-fit ​​groove inside the test tube support frame.

[0008] As a further optimization of this utility model, the adjustable air pump's extraction end is fixedly connected to the inside of the vacuum chamber, and a pressure gauge is provided on the outside of the extraction end. A liquid collection bottle is provided inside the adjustable air pump, and the liquid collection bottle is connected to the adjustable air pump through a filter tube.

[0009] As a further optimization of this utility model, the liquid storage tank is located in the inner bottom wall of the vacuum chamber, and the edge of the liquid storage tank is in a sloping state.

[0010] As a further optimization of this utility model, the inlet end of the drain regulating pipe is fixedly connected to the inside of the storage tank, and the liquid guide pipe is made of a telescopic flexible hose.

[0011] Compared with the prior art, the beneficial effects of this utility model are: In this invention, a horizontal gap of 6-10cm between the two rows of guide tubes provides ample operating space for operators to install / remove extraction tubes and add samples, avoiding collisions and interference when processing multiple tubes in parallel. The liquid storage tank on the bottom wall of the vacuum chamber adopts a sloping edge design, which can guide the waste liquid to quickly converge. Combined with the drain regulating pipe and the guide tube made of telescopic flexible hose on the outside of the vacuum chamber, the waste liquid can be discharged in a directional manner without the need for an additional waste liquid box. Moreover, the telescopic characteristics of the guide tube make it easy to adapt to external collection containers of different heights, making the operation flexible and avoiding waste liquid leakage and pollution, thus reducing the complexity of experimental operations. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the internal structure of the vacuum chamber of this utility model; Figure 3 This is a schematic diagram of the external structure of the flow regulating valve of this utility model; Figure 4 This utility model Figure 2 Enlarged view of the structure at point A in the middle;

[0013] In the diagram: 1. Base; 2. Vacuum chamber; 3. Extraction mechanism; 31. Sealed top cover; 32. Guide tube; 33. Flow regulating valve; 34. Extraction tube; 4. Test tube support frame; 5. Adjustable air pump; 6. Drainage mechanism; 61. Storage tank; 62. Drainage regulating tube; 63. Guide tube. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0016] Please see Figures 1-4 This utility model provides a technical solution: A solid-phase extraction device includes a base 1, a vacuum chamber 2 fixedly connected to the top of the base 1, an extraction mechanism 3 installed on the top of the vacuum chamber 2, a test tube support frame 4 installed inside the vacuum chamber 2, an adjustable air pump 5 installed on the outside of the vacuum chamber 2, and a draining mechanism 6 installed at the bottom of the vacuum chamber 2. The extraction mechanism 3 includes a sealing cover 31, a guide tube 32 is snapped onto the top of the sealing cover 31, a flow regulating valve 33 is fixedly connected to the top of the guide tube 32, and an extraction tube 34 is snapped onto the top of the flow regulating valve 33. The draining mechanism 6 includes a liquid storage tank 61, a drain regulating pipe 62 fixedly connected to the outside of the vacuum chamber 2, and a liquid guide pipe 63 fixedly connected to the outside of the drain regulating pipe 62.

[0017] It should be noted that: the sealing cover 31 is installed on the top of the vacuum chamber 2, the guide tubes 32 are evenly distributed inside the sealing cover 31, the horizontal gap between the two rows of guide tubes 32 is set to 6-10cm, the test tube support frame 4 is divided into upper and lower layers, and the two are connected by a column.

[0018] Furthermore: the bottom of the guide tube 32 is located inside the vacuum chamber 2, the test tube support frame 4 has a snap-fit ​​groove inside, the bottom of the guide tube 32 is located directly above the snap-fit ​​groove inside the test tube support frame 4, the extraction end of the adjustable air pump 5 is fixedly connected to the inside of the vacuum chamber 2, and a pressure gauge is provided on the outside of the extraction end. The adjustable air pump 5 has a liquid collection bottle inside, and the liquid collection bottle is connected to the adjustable air pump 5 through a filter tube.

[0019] Specifically: the liquid storage tank 61 is located in the inner bottom wall of the vacuum chamber 2, and the edge of the liquid storage tank 61 is in a sloping state. The liquid inlet end of the drain regulating pipe 62 is fixedly connected to the inside of the liquid storage tank 61. The liquid guide pipe 63 is made of a telescopic flexible hose. The volume of the vacuum chamber 2 is 30%-50% larger than that of the shell of the traditional manual solid phase extraction device to meet the needs of processing multiple samples at the same time.

[0020] As a further implementation of this scheme, after all samples have flowed through the extraction tube 34, the target component has been adsorbed onto the adsorbent. At this point, a rinsing operation is required to remove impurities adsorbed on the packing surface. The adjustable air pump 5 is turned off, the negative pressure supply is paused, and rinsing reagent is added from the top of the extraction tube 34. The air pump is restarted, and the negative pressure is adjusted to 0.02 MPa. The flow regulating valve 33 is slowly opened to control the rinsing reagent flow rate at 1-2 mL / min. The rinsing reagent flows from top to bottom through the adsorbent packing, interacting with the impurities on the packing surface and eluting them off. The target component, due to its stronger binding force with the adsorbent, remains in the packing. The waste liquid containing impurities generated during rinsing enters the storage tank 61 through the guide tube 32, merging with the previous sample impurity waste liquid. This process must ensure that the rinsing reagent completely flows through the entire packing layer to avoid impurity residue affecting the purity of the target component in subsequent applications.

[0021] Workflow: Before performing solid-phase extraction, the basic assembly and status check of the device must be completed: Place the base 1 stably on the experimental table. The vacuum chamber 2 fixed on top of it provides a closed negative pressure environment for the entire extraction process. It is necessary to ensure that there is no air leakage at the connection between the vacuum chamber 2 and the base 1. The test tube support frame 4 inside the vacuum chamber 2 is fixed by the column. The upper and lower locking slots must be kept horizontally aligned, and each locking slot is located directly below the guide tube 32 of the upper sealing cover 31 to ensure accurate subsequent liquid dripping. The extraction end of the adjustable air pump 5 has been connected to the vacuum chamber 2. It is necessary to check the sealing of the air pump and the air chamber connection pipe, and at the same time confirm that the liquid collection bottle inside the air pump is empty and the filter tube is not blocked. The liquid storage tank 61 in the draining mechanism 6 is in a dry and clean state. The valve on the drain regulating pipe 62 is closed, and the end of the guide tube 63 is aligned with the external waste liquid collection container. The assembly of the extraction mechanism 3 must be carried out in a specific order: First, the sealing cover 31 is tightly fastened to the top of the vacuum chamber 2, and the chamber is sealed by the sealing ring; then, the guide tube 32 is vertically inserted from the snap-fit ​​hole at the top of the sealing cover 31 until the bottom of the guide tube 32 extends into the vacuum chamber 2 and is about 2-3 cm away from the upper snap-fit ​​groove of the test tube support frame 4; then, the flow regulating valve 33 is fixed at the top of each guide tube 32 and the valve is adjusted to the fully closed state; finally, the extraction tube 34 containing the adsorbent packing is snapped onto the top of the flow regulating valve 33 to ensure that the extraction tube 34 is tightly connected to the valve and there is no risk of liquid leakage. Depending on the properties of the sample to be processed, such as water quality, food extract, or pharmaceutical solution, pretreatment is performed in advance, including filtration to remove suspended solids and pH adjustment. The pretreated sample is then aliquoted into clean containers for later use. Simultaneously, the extraction tube 34 is activated: the adjustable air pump 5 is turned on, and the pressure inside the vacuum chamber 2 is adjusted to a negative pressure of 0.02-0.03 MPa using a pressure gauge. Activating reagents, such as methanol or acetonitrile, are slowly added from the top of the extraction tube 34. The corresponding flow control valve 33 is slowly opened, controlling the reagent flow rate to 1-2 mL / min. Under negative pressure, the reagent flows from top to bottom through the adsorbent packing material inside the extraction tube 34, fully wetting the packing pores and removing air from the packing material, preparing for subsequent sample adsorption. The waste liquid generated during the activation process drips through the guide tube 32 into the receiving test tube in the upper slot of the test tube support frame 4. If the activation waste liquid needs to be collected separately, it can drip directly into the bottom storage tank 61 of the vacuum chamber 2. If the activation waste liquid can be combined with the subsequent extraction waste liquid, it is necessary to choose whether to place a receiving container on the test tube support frame 4 according to the experimental requirements.

[0022] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A solid-phase extraction apparatus, comprising a base (1), characterized in that: A vacuum chamber (2) is fixedly connected to the top of the base (1), an extraction mechanism (3) is installed on the top of the vacuum chamber (2), a test tube support frame (4) is provided inside the vacuum chamber (2), an adjustable air pump (5) is installed on the outside of the vacuum chamber (2), and a draining mechanism (6) is provided at the bottom of the vacuum chamber (2). The extraction mechanism (3) includes a sealing cover (31), a guide tube (32) is snapped onto the top of the sealing cover (31), a flow regulating valve (33) is fixedly connected to the top of the guide tube (32), and an extraction tube (34) is snapped onto the top of the flow regulating valve (33). The draining mechanism (6) includes a storage tank (61), a drain regulating pipe (62) is fixedly connected to the outside of the vacuum chamber (2), and a guide pipe (63) is fixedly connected to the outside of the drain regulating pipe (62).

2. The solid-phase extraction apparatus according to claim 1, characterized in that: The sealing cover (31) is installed on the top of the vacuum chamber (2), and the guide tubes (32) are evenly distributed inside the sealing cover (31).

3. The solid-phase extraction apparatus according to claim 1, characterized in that: The horizontal gap between the two rows of guide tubes (32) is set to 6-10cm. The test tube support frame (4) is divided into upper and lower layers, and the two are connected by a column.

4. The solid-phase extraction apparatus according to claim 1, characterized in that: The bottom of the guide tube (32) is located inside the vacuum chamber (2), and the inside of the test tube support frame (4) is provided with a snap-fit ​​groove. The bottom of the guide tube (32) is located directly above the snap-fit ​​groove inside the test tube support frame (4).

5. The solid-phase extraction apparatus according to claim 1, characterized in that: The extraction end of the adjustable air pump (5) is fixedly connected to the inside of the vacuum chamber (2), and a pressure gauge is provided on the outside of the extraction end. A liquid collection bottle is provided inside the adjustable air pump (5), and the liquid collection bottle is connected to the adjustable air pump (5) through a filter tube.

6. The solid-phase extraction apparatus according to claim 1, characterized in that: The liquid storage tank (61) is located in the inner bottom wall of the vacuum chamber (2), and the edge of the liquid storage tank (61) is in a sloping state.

7. The solid-phase extraction apparatus according to claim 1, characterized in that: The inlet end of the drain regulating pipe (62) is fixedly connected to the inside of the storage tank (61), and the liquid guide pipe (63) is made of a telescopic flexible hose.

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